KR20190012337A - Cosmetic Composition Containing Fermented Soy Bean Milk for Antioxidant and for Improvement of atopy - Google Patents

Abstract

The present invention relates to a cosmetic composition having an antioxidant effect and an effect of improving itching caused by skin dryness, which comprises fermented soybean milk as an active ingredient obtained by fermenting soybean milk with a strain of Bacillus amyloliquefaciens. The cosmetic composition comprising the fermented soybean milk produced according to the present invention has excellent antioxidant and moisturizing effects, and thus can be used as cosmetics for antioxidation, anti-aging, wrinkle amelioration and improvement of atopic dermatitis.

Description

[0001] The present invention relates to a cosmetic composition for improving antioxidative and atopic sensation containing soybean oil fermented product,

The present invention relates to a cosmetic composition comprising a soybean fermented product as an active ingredient, and more particularly to a cosmetic composition comprising soybean milk fermented product obtained by fermenting soybean milk with a Bacillus amyloliquefaciens strain, The present invention relates to a cosmetic composition having an atopy improving effect.

Soybean is a food material containing abundant vegetable protein. Soybean milk is an oil beverage obtained by eluting proteins and other components from soybeans by removing water and so forth from soybeans, and is a health drink of low cholesterol and high protein. have. Soy milk contains active ingredients such as isoflavin and has been known to have skin improving effect and lightening effect and has been used for skin for a long time (Korean Patent Registration No. 10-0505322).

In recent years, cosmetics using a fermentation broth of a natural strain such as yeast have been developed, and natural fermentation materials such as yeast and the like have been developed. There is an increasing interest in.

It is known that there are various effects when bio-converting a herbal cosmetic material with a microorganism or an enzyme. First, since the size of many substances contained in a cosmetic material is small and the particle size of a general cosmetic material is a um unit size, While the particle size of the fermented cosmetics is 30 to 70 nm, which increases the absorption capacity. In addition, microorganisms and enzymes remove sugars from the glycosides present in plants and turn them into substances with greatly improved functions. Microorganism fermentation produces beneficial nutrients such as vitamins and organic acids.

As a technique for using a fermented soybean milk product as a cosmetic, microorganisms of the genus Rhizopus are used (JP-A No. 1-10211) or lactic acid bacteria (JP-A No. 3-127715). Also disclosed is an extract of a fermented soybean fermentation extract which is effective for improving rough skin and wrinkles by extracting a fermentation product obtained by reacting a microorganism belonging to the genus Bifidobacterium with a soybean extract solution with an organic solvent (Korean Patent No. 10 -0577914). Korean Patent Laid-Open Publication No. 2003-61447 discloses an external preparation for skin which contains a soybean milk fermentation product and vitamin A or a derivative thereof to prevent roughness and keratinization of the skin.

However, it is possible to efficiently produce useful peptides, amino acids and antioxidants by easily using or decomposing soybean milk protein contained in soybean milk, selecting strains that can be easily proliferated, and providing fermented products having excellent functions such as skin soothing, antioxidation, Production methods have not yet been established.

Accordingly, the present inventors have made intensive efforts to develop a cosmetic composition containing soybean milk fermented product having excellent antioxidation and atopy improving function. As a result, the present inventors have found that soybean milk fermented by soybean milk fermented with Bacillus sp. Isolated from protein- The inventors of the present invention have found that the cosmetic composition containing the antioxidant and the antioxidant is excellent in the antioxidant activity and the itching improving activity by drying.

An object of the present invention is to provide a cosmetic composition containing a soybean fermented product excellent in antioxidant and anti-aging function.

In order to achieve the above object, the present invention provides a cosmetic composition comprising soybean milk fermented product as an active ingredient, characterized in that soy milk is fermented with a Bacillus amyloliquefaciens strain.

The cosmetic composition containing the soymilk fermented product produced according to the present invention has excellent antioxidative and moisturizing effects and can be used as a functional cosmetic for whitening, antiaging and wrinkle improvement.

FIG. 1 shows the results of screening of Bacillus amyloliquefaciens K3 isolated in the present invention.
Figure 2 is a schematic representation of a process for preparing a fermented soybean milk according to the present invention.
FIG. 3 shows the results of analysis of the antioxidative effect of the soybean milk fermented product according to the present invention.
FIG. 4 shows the results of dermal moisture change after using the cosmetic product containing the soybean milk fermented product according to the present invention.
FIG. 5 is a graph showing changes in a * value indicating redness of skin after use of a cosmetic product containing soybean milk fermented according to the present invention.

When biomaterials are biotransformed or fermented using microorganisms or enzymes, the microorganisms or enzymes decompose the proteins and glycoside materials present in the biomaterials and convert them into substances with improved functions. By fermenting microorganisms, antioxidant peptides, Antioxidant amino acids, vitamins, and organic acids. In the present invention, in order to isolate a strain having a high protease activity for fermenting soybean milk rich in protein and containing an active ingredient such as isoflavin and fermenting soybean milk with the strain, soybean oil fermented product And the cosmetic composition containing the soybean milk fermented product showed antioxidative and skin irritation-improving effects.

Accordingly, the present invention relates to a cosmetic composition comprising a soybean milk fermented product as an active ingredient, characterized in that soybean milk is fermented with a strain of Bacillus amyloliquefaciens .

In the present invention, the cosmetic composition may be characterized by having a function selected from the group consisting of whitening, anti-aging, wrinkle improvement, and atopic dermatitis improvement.

In the present invention, a GRAS microorganism capable of efficiently decomposing a large amount of proteins contained in soymilk and capable of being converted into a functional substance was selected and it was confirmed that the Bacillus strain isolated from Kimchi using a canary broth having a high proteolytic activity, It was isolated and identified as Bacillus amyloliquefaciens K3. Bacillus amyloliquefaciens K3 was identified as Bacillus amyloliquefaciens K3.

Therefore, in the present invention, the strain may be characterized by being Bacillus amyloliquefaciens K3.

The method for producing the soybean milk fermented product of the present invention can be carried out through the following steps.

(a) immersing in water to pulverize the so-called soybeans;

(b) heat treating the pulverized soybeans, separating the beans and obtaining soybean milk; And

(c) Inoculating the soy milk with Bacillus amyloliquefaciens K3 to obtain a soybean fermentation broth.

In the present invention, the fermentation may be carried out for 4 to 48 hours, preferably 4 to 24 hours, more preferably 4 to 10 hours, at 37 ° C .

The obtained soybean fermentation broth can be used as a soybean fermentation product by lyophilization.

The soy milk to be used in the present invention may be any one obtained by a conventional method, or a commercially available soy milk may be used. For example, it is more preferable to use a liquid obtained by growing or pulverizing soybean curd soaked in soybeans or soybean swollen by swelling in water, and using the liquid from which the soybeans are removed. In addition, a solution obtained by dissolving whole soybean powder, defatted soybean powder or the like may be used. In the JAS standard, soybean solids of 8.0% or more are defined as soybean milk, crude soybean milk is 6.0% or more, and soybean milk drink is 4.0% or more, but soybean solid content is not particularly limited.

The method of fermenting soybean milk with lactic acid bacteria can be carried out on the basis of a generally known method of producing yogurt or lactic acid bacteria beverage. For example, the method of fermenting soy milk with lactic acid bacteria may be carried out by mixing the bacillus amyloliquefaciens with 1 10 ^{4 to} 1 10 ⁸ cfu / mL , More preferably 1 to 10 ^{5 to} 1 to 10 ⁷ cfu / mL, and the fermentation form is not particularly limited.

The fermented soybean milk prepared by the method of the present invention showed that the essential amino acids such as amino acids and Lys, Trp and Phe were increased and the aromatic amino acid, which is a precursor of the physiologically active substance, was increased as compared with soybean milk.

The present invention is characterized in that the content of the soybean fermented product is 0.001 to 30.0% by weight based on the total weight of the cosmetic composition, and when the content of the extract is less than 0.001% by weight, %, The effect of increasing the content is insignificant, which is not economical.

The present invention relates to a soybean milk complex fermented extract which is characterized in that the cosmetic composition is selected from the group consisting of lotion, gel, water-soluble liquid, cream, essence, oil (O / W) As an active ingredient.

The cosmetic composition containing the composition of the present invention as an active ingredient can be easily produced in any form according to a conventional production method. For example, in the preparation of a cream type coating agent, the natural composition of the present invention is contained in a cream base of a general water type (O / W) or water type (W / O), and a flavor, a chelating agent, a colorant, an antioxidant, Etc. may be used as needed, and synthetic or natural materials such as proteins, minerals, and vitamins may be used in combination for the purpose of improving physical properties. In addition, the cosmetic composition of the present invention can be formulated into a cosmetic, a gel, a water-soluble powder, a fat-soluble powder, a water-soluble liquid, a cream or an essence by a conventional method of manufacturing a cosmetic by adding a pH adjusting agent, a flavoring agent, an emulsifier, .

A cosmetic composition is formed with the useful substance. Materials useful for cosmetics include, but are not limited to, antioxidants, binders, bulking agents, chelating agents, pigments, softeners, emulsion stabilizers, film formers, fillers, directional ingredients, gelling agents, hair conditioners, hair fixatives, , Preservatives, skin conditioners, solvents, sunscreen agents, surfactants, ultraviolet absorbers, viscosity modifiers, waxes, and the like. A number of cosmetically useful materials are described in the CTFS Cosmetic Ingredient Handbook, J.M. Nikitakis, Ed., 1st Edition, p. 51-101 (1988), all of which are hereby incorporated herein by reference.

The cosmetic composition of the present invention may comprise a composition comprising a wax.

The soybean fermented product produced by the present invention relates to a cosmetically acceptable carrier. The carrier may be an aqueous solution such as water or a polar organic solvent, an alcohol such as ethanol or other polar solvent, a natural or synthetic oil; Oil-in-water emulsion; Water-in-oil emulsion; Or wax. The carrier is clearly non-toxic. A preferred carrier for the composition of the present invention is an aqueous solution.

Depending on the function, the cosmetic composition of the present invention can be used in a variety of forms such as solutions (lotion-like compositions), concentrated solutions, gels, ointments, emulsions (creams, emulsions), vesicular dispersions, powders, dense powders, pastes, And the like. More specifically, the cosmetic composition of the present invention can be used as a cosmetic composition for oral use such as ball puff, cream (facial cream, hand cream, moisturizing cream, sunscreen cream), cream powder, eyeliner, eye shade, eyebrow pencil, foundation, lotion, Emulsions, ointments, pomades, lozes, and the like, but are not limited thereto. They may be packaged in a squeeze pack containing a propellant that can be applied in the form of a foam or spray.

The soybean milk fermentations described herein can be added to various types of cosmetic compositions. These include, for example, pharmaceutical compositions that protect epidermis, hair, and mucous membranes; Makeup compositions for skin and surface area body creams; A tooth whitening agent and the like, or an ophthalmic composition such as a colgria.

The soybean fermented product of the present invention may contain a cosmetically effective amount and, if used in a pharmaceutical composition, will contain a therapeutically effective amount as described herein. Suitably they are present in the compositions of the present invention in an amount of from about 0.01 to about 50%, more preferably from about 0.02 to about 10%, and most preferably from about 0.05 to about 2% of the total composition weight.

When the cosmetic composition of the present invention is used for hair protection, they may be shampoos, lotions or gels; May be provided in the form of a composition before or after rinsing or shampooing, before or after dyeing or discoloring, during wave medicine or straight treatment, before and after the composition. They may also be provided in the form of a hair styling or treating lotion or gel, a gel for lotion or blow dyeing or hair setting, a hair spray, a composition for wave or straight parmenans, or a hair dyeing or decolorizing composition.

When the composition of the present invention is used as an eyelash, eyebrow, or makeup product for skin, they may be provided in the form of a foundation, a lipstick, an eye shadow, an eyeliner, a mascara or a skin cream.

When the composition of the present invention is used as a pharmaceutical composition such as an antimicrobial composition, a composition for removing wrinkles and facial hair lines, a moisturizer, etc., they are provided in the form of emulsion, gel, lotion, ointment, And may contain a pharmaceutically active ingredient.

The cosmetic composition may contain natural or artificial wax. Natural waxes include animal and carnauba such as lanolin, beeswax, spermaceti or lanolin alcohol, hardened or acetylated lanolin, lanolin derivatives such as lanolin fatty acids or acetylated lanolin alcohols, carnauba, candelilla, There are vegetable such as kapok, rice, hardened jojoba, alfa or yaguan wax or cork fiber, sugar cane wax, cocoa butter and the like. Alternatively, the inorganic wax may include paraffin, montan, lignite, petrolatum, petrolatum wax or microcrystalline wax, ceresin, or ozokerite. Examples of synthetic waxes used include linear esters such as myristyl myristate, a reaction product of a polyethylene wax obtained by Fischer-Tropsch synthesis and a saturated C10-C40 carboxylic acid and a saturated C10-C40 alcohol. Other waxes used include calcium lanolate or stearate or hardened coconut oil and the like.

The cosmetic composition may include modified or unmodified oils of vegetable or animal oils. For example, sweet almond oil, avocado oil, castor oil olive oil, jojoba oil, sunflower oil, turmeric oil, sesame oil, peanut oil, grape seed oil, soy milk, safflower oil, coconut oil, maze oil, hazelnut oil, Butter, palm oil, apricot seed oil, calf pill oil or perhydro-squalene. Furthermore, the oil phase may be an inorganic oil such as liquid paraffin, liquid petrolatum, and the like. The oil may be selected from the group consisting of fatty acid esters such as isopropyl myristate, isopropyl palmitate, 2-ethylhexyl palmitate, penicillin oil (stearyloctonate), unsaturated fatty acids such as oleate, palmitate, stearate, behen, linoleic, Volatile or non-volatile isoparaffins such as fatty acids or C8-C16 isoparaffins.

Moreover, the oil may be C12-C18 fatty alcohols such as oleyl alcohol, cetyl alcohol and stearyl alcohol.

If provided as an emulsion, the emulsified composition of the present invention comprises an oil phase and an aqueous phase. The oil phase is preferably provided in a range of about 1 to about 75 wt%, more preferably about 5 to about 60 wt%, and most preferably about 40 to about 60 wt%, based on the total weight of the composition desirable.

The aqueous phase includes aqueous gels and adjuvants commonly used in cosmetic emulsions. The aqueous phase may be provided at from about 0.5 to about 20 weight percent, and may include a lower C2-C6 monoalcohol and / or a polyol such as glycerol, butylene glycol, isoprene glycol, propylene glycol, ethylene glycol, and the like.

Emulsifiers may be used to prepare emulsified cosmetic compositions. Any amount of cosmetically acceptable emulsifier may be used as long as it exhibits the desired emulsion effect. Emulsifiers are selected from known salts and surfactants. The emulsifier is preferably selected from stearic acid, sorbitan sesquioleate, polyethylene glycol (PEG-30), dipolyhydroxystearate, lecithin, magnesium stearate, and derivatives and mixtures thereof. The emulsifier is preferably present in the range of about 0.5 to about 30 wt.%, More preferably about 1 to about 12 wt.%, And more preferably about 4 to about 8 wt.%, Based on the total weight of the composition desirable.

The present invention can also use a thickener. It is possible to use any symptom that is normally used in cosmetics. Examples include hectorite, crosslinked polyacrylic acid and guar gum modified with modified magnesium silicate (Bentoncel VS38, Rheox), distearyldimethylammonium chloride (Bentone 38 CE, Rheox) and modified or unmodified cellulose And the like.

The composition of the present invention may comprise a film forming composition. The film-forming composition may be, for example, an aqueous dispersion of an aqueous dispersion of an acrylic, polyester and / or polyurethane polymer, for example an aqueous dispersion of a partially neutralized vinyl acetate / vinyl p-tert-butyl / benzoate / Can be selected in an aqueous dispersion of the same polymer

The composition of the present invention may contain a coloring agent. Such a colorant may be an inorganic or organic pigment insoluble in an aqueous solution or an organic solvent, and may be an aqueous solution or a dye soluble in an organic material.

Lubricants are another optional ingredient. Lubricants generally help the composition to create a soft, smooth feel in the hand. Examples include benzoic acid esters of C12-C15 alcohols, volatile silicones such as cyclomethicone, dimethicone, and derivatives thereof. Silicon containing compounds that may be utilized in the present invention include nonvolatile silicones such as cyclomethicon tetramer and pentamer (obtained as Dow Corning 244 or 245 fluid) or stearyl dimethicone (obtained as Dow Corning 2503 cosmetic wax) Dicetyl polyol, dimethicone polyol, cetyl dimethicone copolyol, phenyl methicone, and derivatives thereof such as phenyl trimethicone. Those skilled in the art will understand that the silicon-containing compound acts as an emulsifier.

The silicon-containing compound is preferably provided in a range of about 1 to about 50 wt%, more preferably about 5 to about 30 wt%, and most preferably about 10 to about 25 wt%, based on the total weight of the composition .

A moisturizing agent may also be added to the cosmetic composition. &Quot; Humectant " refers to a reagent that provides a moisturizing effect to the skin such as a wetting agent.

The moisturizing agent is provided with a moisturizing amount. Examples of the moisturizing agent include glycerin, butylene glycol, propylene glycol, sorbitol, sodium PCA, Glucam E-10, Glucam E-20 and the like. The moisturizing agent is preferably provided in a range of about 0.1 to about 10 wt%, more preferably about 0.1 to about 5 wt%, and most preferably about 1 to about 5 wt%, based on the total weight of the composition Do.

In addition, a softener may be included as needed. It is preferred that at least one softening agent is used in the composition. Softeners are used to give the skin surface a soft, smooth feel. They can be effective without significantly affecting skin hydration levels and / or skin liquefaction barriers. Examples of softeners include organic acid esters such as vegetable triglycerides such as avocado oil, olive oil and sunflower oil, sorbitan oleate, myristyl myristate, isopropyl myristate, glycol oleate, petroleum, glycerin, petrolatum, mineral oil jelly And the like. The softening agent is preferably present in the range of about 1 to about 50%, more preferably about 5 to about 40%, and most preferably about 10 to about 25% by weight of the composition.

In addition, the cosmetic composition of the present invention may contain an antioxidant. Antioxidants may be natural or synthetic. Examples include vitamin E derivatives such as tocopherol, vitamin E linoleate, vitamin E, vitamin E POE succinate, vitamin E acetate, ascorbic acid, ascorbyl palmitate, and ascorbyl PMG. For example, an effective amount is used to neutralize harmful oxides such as a single oxygen species. They are preferably provided in the range of about 0.1 to about 5 wt%, more preferably about 0.5 to about 1 wt%.

The cosmetic composition may contain ingredients that reduce the oily feel of the softener in the composition such as, for example, known creams such as PPG-2-myristyl ether propionate, isopropyl palmitate, and the like. If this component is required, it is preferably provided in an amount of about 1 to about 30%, more preferably about 5 to about 25% by weight, and most preferably about 10 to about 25% by weight.

A pH adjuster is generally used to adjust the acidity of the composition to a desired range, preferably about 6 to 8 pH.

An example is aminomethyl propanol. This pH adjusting agent is added in an effective amount to change the pH to the desired pH range.

It is preferred to use less than 0.4% by weight of the total weight of the composition.

Sunscreens can be included as needed. The expression " sun blocker " means useful for absorbing, blocking or preventing ultraviolet light penetrating the skin. Sunscreen agents are preferably sunscreen agents coated with titanium dioxide, or zinc oxide, more preferably polymeric materials or cosmetically acceptable coatings. Examples of sunscreen agents include microcrystalline titanium dioxide coated with aluminum stearate, polyfluoroalkyl phosphates of C9-C15, polymers coated with zinc oxide, aminobenzoic acid (PABA) and esters thereof, benzophenone-3, octyl Salicylate, menthyl anthranylate, phenylbenzimidazole sulfonic acid, and the like.

More specifically, sunscreen agents provide protection indexes (SPF) of at least 2-12, intermediate 12-30, and up to 30 or greater.

The equilibrium of the composition can be determined by conventional additives such as additives, fillers, organic solvents (alcohol and oil), buffer solutions, perfumes, tanning agents (dihydroxyacetone) and other cosmetically acceptable carriers And a filler. Other conventional additives that can be used in the cosmetic compositions of the present invention include dispersants and preservatives. The amount of conventional additives is typically provided in the range of from about 0 to about 20 weight percent, preferably from about 1 to about 15 weight percent, and more preferably from about 4 to about 10 weight percent, based on the total weight of the composition .

It will be appreciated that the remaining percentage or equilibrium of the composition is water. Water acts as a carrier to disperse the composition into the skin. The water used is preferably deionized water or distilled water, and the amount of water is typically about 10 to 80 wt%, most preferably about 20 to 40 wt%.

In a preferred embodiment, the cosmetic composition of the present invention is a cream or lotion or ointment, most preferably a cream. The carrier is preferably water. The preferred cream composition is formulated as an oil-in-water emulsion containing essential cosmetic ingredients known to those skilled in the art to ensure high quality even with the addition of soy milk fermented ingredients. In preferred embodiments, such ingredients include petrolatum in the range of from about 0.5 to about 1 weight percent of the composition, especially white petrolatum, lanolin alcohol in the range of from about 0.5 to about 1 weight percent of the composition, PPG-2-myristyl ether propionate in the range of about 6 weight percent, petroleum in the range of about 5 to about 10 weight percent of the composition, triethanol amine in the range of about 0.2 to about 0.8 weight percent of the composition, Glycerol in a range of from 2 to about 4 weight percent, particularly distilled glycerol, stearic acid in a range of from about 15 to about 20 weight percent of the composition, isopropyl palmitate in a range of from about 10 to about 15 weight percent of the composition And the balance is water. Cosmetics or pharmaceutical compositions may contain conventional components known to those skilled in the art, as well as fragrances such as antioxidants and coloring agents, perfumed vitamins, as well as those described above, depending on the particular cream form desired.

The cosmetic composition of the present invention comprising the soybean fermented product prepared as described above has several functions. First, they exhibit a remarkable restorative effect of the skin, as described in some hydraulic embodiments of the present invention.

The compositions of the present invention can be applied to the skin daily and, preferably, more than once a day. In order to achieve the stated stable recovery effect, periodic application should be applied for several weeks or even months, even if the initial several times of application show visible effect. The speed and duration of application depends on the concentration of the active ingredient. Those skilled in the art should be able to design suitable application methods to provide the required restoration effect.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

Example 1: High-activity proteolytic enzyme-containing fermentation strain screening for soybean milk fermentation

For the detection of useful microorganisms, microorganisms were isolated from fermented foods such as salted fish, and then microorganisms with high protease activity were searched.

The result was a one K3 microorganisms isolated from sand eel Salted other as compared with the higher microbial activity of the protease, and was identified to be Bacillus amyloliquefaciens it was identified, designated as Bacillus amyloliquefaciens K3 (Fig. 1).

Bacillus amyloliquefaciens K3 was cultured in Trypic soy broth at 37 ℃ for 24 hours and then stored by freeze drying.

Example 2: Preparation of soybean milk fermentation product using fermentation strain

Soybean was soaked to make soymilk, and then immersed in water for 14 hours. Then, water was removed, and the soybean milk was ground using a grinder to remove water. The ground sample was boiled at 100 DEG C for 30 minutes, put into a bag, pressed to separate the beef, and soymilk was obtained.

Bacillus amyloliquefaciens K3 strain, which was lyophilized in the obtained soy milk, was added at 0.1% and cultured at 37 ° C for 6 hours to produce a soybean fermented product.

The prepared liquid soybean milk fermented product was lyophilized to prepare a soy milk fermented product in a powdery state.

Example 3: Analysis of amino acid composition of fermented soybean milk

The amino acid composition of the soybean milk fermented product prepared in Example 2 and the pre - fermented soybean milk were analyzed by HPLC to compare the amino acid composition of the soybean milk fermented product and the soybean milk before fermentation.

As a result, the essential amino acids such as amino acids and Lys, Trp and Phe were increased, and the aromatic amino acid, which is a precursor of the physiologically active substance, was increased compared to soybean milk (Table 1).

B. Amyloliquefaciens K3 fermentation of soybean milk amino acids soy milk( ug / ml) soy milk Fermentation product ( ug / ml) Essential
Amino acid HIS 5.1 74.5 VAL 10.4 115.7 ILE 1.4 142.5 LEU 1.5 179.2 MET 38.7 99.3 THR 1.4 38.7 LYS 12.9 594.9 TRP 24.1 231.1 PHE 17.2 226.4 Non-essential amino acids
TYR 2.2 135.0 ASN 8.5 24.3 SER 2.4 24.2 PRO 3.8 4.7 GLU 39.1 144.2 GLY 4.4 17.6 ALA 14.1 53.5 ASP 31.0 6.8 GLN 0.8 23.6 CYS 0.0 0.0 ARG 117.4 164.5 TOTAL 336.3 2300.6

Example 4: Antioxidant activity analysis of soybean milk fermented product

A soybean fermented product was prepared from soybean and black soybean as a raw material by the method of Example 2.

The prepared fermented soybean curd soybean milk and fermented soy sauce soybean milk were subjected to HPLC analysis to identify antioxidant substances contained in soy milk fermented milk. Peptide fractions were separated and analyzed for antioxidative and DPPH radical scavenging activity of each fraction. The DPPH radical scavenging activity was measured by using a spectrophotometer to measure the degree of reduction of the DPPH radical by reacting a stable free radical, 1.1-diphenyl-2-picryl hydrazyl (DPPH) with a certain sample solution. , 1 ml of distilled water, and 0.035% DPPH or 100 μl of methanol. The mixture was allowed to stand at room temperature for 10 minutes, and the absorbance at 517 nm was measured to calculate the degree of radical reduction. As a standard sample, ascorbic acid was used.

As a result of DPPH radical scavenging analysis on the samples fractionated by HPLC, three fractions having excellent antioxidative activity were selected as shown in Fig. 3. (fraction of 39, 44, 45)

Example 5: Confirmation of skin irritation of cosmetic composition containing soybean milk fermented product

Cream, lotion and shampoo formulations containing soymilk fermented products were evaluated for human skin irritation to cosmetic compositions.

The human body test was conducted by the Korea Dermatological Institute.

The skin patch test was conducted in 33 creams, lotions and shampoo formulations containing 0.1% soybean milk fermented by the method of Example 2.

Lotion containing soybean fermented product Raw material name Content (% by weight) Cetearyl alcohol 1.0 Chin type glyceryl stearate 1.0 Glyceryl stearate SE 1.5 Phitosqualan 3 Hydrogenated Polydecenes 2 Dimethicone 0.5 Polysorbate 60 One Sorbitan sesquioleate 0.4 Methyl paraben 0.1 Propylparaben 0.05 Butylene glycol 5 Polyacrylate-13 * polyisobutene * polysorbate 20 0.5 Fermented soy milk 1.0 Purified water Balance

Cream containing soy milk fermented product Raw material name Content (% by weight) glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount Fermented soy milk 1.0 Purified water Balance

Shampoo containing soy milk fermented product Raw material name Content (% by weight) Ammonium lauryl sulfate solution 28 Ammonium laureth sulfate solution 20 Phenoxyethanol 0.2 Polyquaternium-10 0.4 Cetyl alcohol One Dimethicone One Citric acid 0.1 Trihydroxystearin 0.3 Fermented soy milk 1.0 Purified water Balance

Thirty - three adult male and female subjects underwent a skin patch test using a Finn chamber. The back of the subject was wiped with 70% ethanol and dried. Then, 20 μL of the test material was dropped on a Finn chamber having a diameter of 8 mm and fixed on the test site. For test material # 3, a filter paper disc was placed in a Finn chamber with a diameter of 8 mm, and then 20 μL of the test material was dropped and fixed on the test site. Test substance # 3 was diluted to 1% concentration in sterilized primary distilled water. Patches were attached for 24 hours. After 30 minutes, 24 hours, and 48 hours after removal of the patches, the dermatologist observed the degree of stimulation according to the criteria of the International Contact Dermatitis Research Group (ICDRG) The mean skin response (Mean score) was calculated according to the judgment table.

As a result, irritation was not observed after 30 minutes, 24 hours, and 48 hours after the removal of the patch. The average skin response was 0.00 and judged to be unstimulated according to the criteria. No stimulation was observed at 30 minutes, 24 hours, and 48 hours after removal of the patch. The average skin response was 0.00 and judged to be unstimulated according to the criteria. No stimulation was observed at 30 minutes, 24 hours, and 48 hours after removal of the patches. The average skin response was 0.00 and judged to be unstimulated according to the criteria.

Therefore, all of the creams, lotions and shampoo formulations are judged to belong to the non-irritating product group as a result of the human body application test for evaluating the skin primary stimulation by the skin patch.

Example 6: Confirmation of skin soothing effect of cosmetic composition containing soybean milk fermented product

The skin soothing effect on the cream containing the soymilk fermented product used in Example 5 was confirmed.

The human body test was conducted by the Korea Dermatological Institute.

The subjects were 22 women who were 12 ~ 60 years old or older who had dry skin and had an average or more dry skin of 60 or less with Corneometer moisture meter.

After washing and washing for 4 weeks during the test period, the same amount of cream, which is a test substance twice a day twice a day, was spread evenly over the facial region and the right forearm region.

The test was conducted in accordance with the Korean Dermatological Institute Standard Work Practice Guide (SOP), and all procedures were checked by a person in charge of reliability assurance.

MoistureMeterD Compact and the results of the test for relieving itching by dryness showed that the dermis moisture was improved by 9.37% after 2 weeks of use and by 10.09% after 4 weeks of use compared to before using the test material (p <.001). (Figure 4)

In addition, as a result of evaluating the degree of improvement of itching relieving by drying using the questionnaire of the subject, results of the behavior and habit state due to itching before and after using the test substance showed that the degree of itching was 2.36 to 2. weeks before use And 1.91 after 4 weeks of use, the frequency of itching was 1.73 before 2 weeks of use and 0.73 after 4 weeks of use. The frequency of itching was 1.18 before use of the test substance and 0.41, The ratio of itching was 0.32 before the test substance, 1.45 after 2 weeks use, 1.18 after 4 weeks use, and the itching degree at 1.77 before the test substance was the lowest (P <.001), respectively, after 1 week of use and 0.82 after 4 weeks of use.

The degree of depression in the psychological state questionnaire of the test subjects using the 10 ㎝ visual analogue scale before and after the use of the test substance was found to be 2.09 before the use of the test substance and 2.09 after the use of the test substance, Before the test substance, it was confirmed that the level of anger was improved from 4.82 before use to 1.95 after 2 weeks use, to 1.05 after 4 weeks use (p <. 001).

The results of skin sedative evaluation using SkinColorCatch and full-screening system JANUS-1 showed that the a * value of redness of skin was 4.83% after 2 weeks use and 5.13% after 4 weeks use compared to before using test substance, (P <.05) (FIG. 5). No skin adverse events were observed from the subject during the test period.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereto will be. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (5)

A cosmetic composition comprising soybean oil fermented product as an active ingredient, characterized in that soy milk is fermented with a strain of Bacillus amyloliquefaciens .
The method according to claim 1, wherein the soybean fermented product is increased in essential amino acids Lys, Trp, Phe and aromatic amino acid.
The cosmetic composition according to claim 1, which has a function selected from the group consisting of antioxidation, anti-aging, wrinkle improvement, itching improvement and atopic dermatitis improvement.
The cosmetic composition according to claim 1, wherein the Bacillus amyloliquefaciens strain is Bacillus amyloliquefaciens K3.

The cosmetic composition according to claim 1, wherein the soybean milk fermented product is obtained through the following steps.
(a) immersing in water to pulverize the so-called soybeans;
(b) heat treating the pulverized soybeans, separating the beans and obtaining soybean milk; And
(c) Inoculating the soy milk with Bacillus amyloliquefaciens K3 to obtain a soybean fermentation broth.

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