KR100981343B1 - The cosmetic composition for the reduction of skin pores - Google Patents

Abstract

The present invention relates to a cosmetic composition for reducing pores, by containing 0.01 to 10.0% by weight of the persimmon leaf extract fermentation broth cultured by inoculating useful microorganisms persimmon leaf extract to the total cosmetic composition to restrain the increase of pores and restore the structure of the enlarged pores By helping to reduce the size of the pores as well as to reduce the amount of sebum secretion can be obtained a cosmetic composition for reducing pores.

Persimmon Leaf, Persimmon Leaf Extract, Persimmon Leaf Extract Fermentation Solution, Pore Reduction, Cosmetics, Composition

Description

Cosmetic composition for reducing pores {The cosmetic composition for the reduction of skin pores}

The present invention relates to a cosmetic composition for shrinking pores, and more specifically, by containing 0.01 to 10.0% by weight of the persimmon leaf extract fermentation broth cultured by inoculating useful microorganisms in persimmon leaf extract and increasing the amount of pores by inhibiting elongation of pores. The present invention relates to a cosmetic composition for reducing pores, which not only helps to reduce the size of pores by restoring the structure of the pores, but also exhibits an excellent effect on reducing sebum secretion.

The skin consists of two parts: the shallow epidermis (epidermis) and the deeper layer below the dermis (dermis), and under the dermis there is a separate layer of connective tissue called subcutaneous tissue. The epidermis and the dermis are clearly distinguished by the basement membrane, but between the dermis and the subcutaneous tissue are all the same connective tissues, leading directly without boundaries. Many of these are characteristic. These skins have skin appendages such as hair follicles, sweat glands, and the like, which are commonly referred to as follicles in which the epidermis, in which the hair grows, is recessed toward the dermis. The pores are the openings where hair grows, and the sebum flows through the pores to the skin surface. The diameter is 0.02 ~ 0.05㎜, different from the pores, there are about 20,000 in the face only.

The reason for the widening of pores is that as the skin ages, the collagen, elastin and structural components that supported the wall of the pores decompose or deteriorate, which reduces the elasticity of the skin, causing the pores to swell or stretch, or the pores are stretched or angled by excessive sebum secretion. The process is not done properly, the pores are blocked by the thickened stratum corneum and inflammation in the hair follicles, which eventually leaves a clear trace. There are many factors that determine the amount of sebum, but hormones are particularly active. The most influential hormone is androgen, a male hormone, which increases the development, synthesis, and size of sebaceous glands.

Such methods for improving pores include pore contraction (Korean Patent No. 1997-70601) and sebum secretion inhibitors (Japanese Patent Application Laid-Open No. Hei 5-294836), but they have only a fractional effect and thus have limited use. Development of material is necessary.

On the other hand, fermentation refers to a process in which microorganisms decompose organic matter using their own enzymes and include alcohol fermentation and lactic acid fermentation. By fermentation of microorganisms and enzymes secreted by microorganisms, the substance is naturally decomposed to less than 80nm on average, so it is easy to absorb the skin. Will have In addition, toxic substances such as heavy metals, residual pesticides, etc., which are harmful to the human body by microorganisms, are completely decomposed and harmless to the human body, and fermentation products are resistant to heat and carbon dioxide and do not deteriorate well, thus increasing the preservation of active ingredients.

Accordingly, an object of the present invention is to provide a cosmetic composition for reducing pores, which suppresses the increase of pores and restores the structure of the enlarged pores, not only helps to reduce the size of the pores, but also shows an excellent effect on reducing the amount of sebum secretion.

In order to achieve the above object as well as other easily expressed object in the present invention by containing the persimmon leaf extract fermentation broth cultured by inoculating useful microorganisms persimmon leaf extract to 0.01 to 10.0% by weight relative to the total cosmetic composition to suppress the pores increase It was possible to obtain a cosmetic composition that not only helps to reduce the size of the pores by restoring the structure of the enlarged pores, but also shows an excellent effect on reducing the amount of sebum secretion.

That is, the present inventors have completed the present invention by revealing that the persimmon leaf extract fermentation broth exhibits an excellent effect on pore convergence and sebum secretion reduction.

The cosmetic composition for reducing pores according to the present invention has an excellent effect on suppressing the increase of pores and restoring the structure of the increased pores to reduce the size of the pores, as well as reducing sebum secretion.

The cosmetic composition for reducing pores according to the present invention is characterized by containing 0.01 to 10.0% by weight of the persimmon leaf extract fermentation broth cultured by inoculating useful microorganisms in the persimmon leaf extract.

Persimmon leaf used in the present invention is a leaf of Diospyros Kaki L., a deciduous arborescent, 6 to 14 m high, the outer shell of the stem is divided into scales, and has brown hairs on small branches. The leaves are alternate, leathery, oval in the shape of an egg. Leaves are 7 ~ 17cm long, 4 ~ 10cm butterfly, no sawtooth, petioles 5 ~ 15mm long, hairy. The back side of the leaf is green and glossy.

Pharmacological effects and efficacy on persimmon leaves are well documented in many ancient literatures, including Dongbobom and Herbal Medicine. Persimmon leaves are effective in adult diseases such as hypertension, arteriosclerosis, heart disease, and chronic diseases such as gastric ulcer, duodenal ulcer, and diabetes, and are rich in vitamins A, C, D, and chlorophyll, which are known to be effective in preventing cancer. In addition, vitamin B1, pantothenic acid, folic acid, and tannins contain a large amount, and the astringent component tannin is known for detoxification, immune function activation, and free radical scavenging action along with various biological activities. Flavonoids isolated from persimmon leaves are also known to inhibit tumor cell proliferation. In particular, persimmon leaves contain 20 times more vitamin C than lemon, so whitening and antioxidant-related effects are known (Korean Patent Publication No. 1998-0055293). However, research on pore convergence and sebum secretion, etc. of persimmon leaf extract has not been made, and furthermore, there is no fact that persimmon leaf extract fermentation broth through microbial fermentation has superior effect than conventional persimmon leaf extract as in the present invention.

Persimmon leaf extract used in the present invention can be prepared as follows.

The persimmon leaves are washed with water, dried in the shade, placed in an extraction container, and extracted at an appropriate temperature for a predetermined time in an appropriate amount of the extraction solvent. The extract may be filtered and concentrated using a vacuum rotary evaporator or the like.

As the extraction solvent, a polar solvent selected from water (purified water), a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof may be used, and preferably water is used.

The amount of the extraction solvent is 2 to 10 times the dry weight of persimmon leaf, the extraction method may be an extraction method, such as bath extraction, reflux cooling extraction, ultrasonic extraction, preferably 1 to 5 times as an extraction method of bath extraction Can be extracted.

Maintaining the extraction temperature is 30 ~ 70 ℃, more preferably 40 ~ 60 ℃, most preferably about 50 ℃ is most effective in the prevention of decay, discoloration and odor, extraction time is 5 to 20 hours, preferably Is 10 hours.

After the persimmon leaf extract is obtained, the solids are removed using a filter paper, the suspension is centrifuged, and the supernatant can be filtered under reduced pressure.

In addition, the filtered extract may be concentrated under reduced pressure at 20 ~ 100 ℃, preferably 50 ~ 70 ℃ with a vacuum rotary concentrator.

Microorganisms that ferment the extract are species commonly used for fermentation, such as yeast (Saccharomyces), fungi (Aspergillus), lactic acid bacteria (Lactobacillus), and at about 25 to 50 ℃ for about 24 to 120 hours, preferably 48 to 72 Fermentation time.

The fermentation broth was filtered or centrifuged to remove extract powder and microorganisms.

Persimmon leaf extract fermentation broth according to the present invention has a pore convergence effect and sebum secretion inhibitory effect and this fact is specified in the following examples.

Cosmetics prepared from a cosmetic composition having a persimmon leaf extract fermentation broth of the present invention having a pore convergence and sebum secretion effect can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition, the persimmon leaf extract fermentation broth of the present invention can be prepared in the form of adjuvants that can be applied topically or systemically used in the field of dermatology by containing a dermatologically acceptable medium or base in addition to cosmetics.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to the persimmon leaf extract fermentation broth, fatty substances, organic solvents, solubilizers, thickening and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, Commonly used in water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics It may contain adjuvants conventionally used in the cosmetic or dermatological fields, such as any other ingredients used. In addition, the above components may be introduced in an amount generally used in the dermatology field.

Examples of products to which the cosmetic composition of the present invention may be added include, for example, cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, etc. Etc.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

In the present invention, the content of the persimmon leaf extract fermentation broth is effective to 0.01 to 30.0% by weight based on the total cosmetic composition, when the amount of persimmon leaf extract fermentation is less than 0.01% by weight can not be expected pores convergence effect and sebum secretion effect. If the content of burdock extract fermentation broth exceeds 10.0% by weight, it is not economical because there is no apparent increase in effect as the content increases.

The following examples illustrate the invention in more detail, but do not limit the scope of the invention.

Production Example  1: Preparation of Persimmon Leaf Extract

1 kg of persimmon leaves were finely crushed in a crusher, immersed in 100 L of water, and warmed in a 50 ° C. water bath for 10 hours, and the resulting extract was cooled to room temperature, filtered through a filter paper to obtain a filtrate. Prepared in powder form by evaporating water from the filtrate with nitrogen.

Production Example  2: Preparation of Persimmon Leaf Extract Fermentation Solution

10 ml of water was added to 10 g of the powder obtained in Preparation Example 1, heated at 121 ° C. for 20 minutes to eliminate various bacteria, inoculated with lactic acid bacteria, fermented at 40 ° C. for 48 hours, and the culture solution was separated through microfiltration.

Experimental Example  1: protein aggregation effect (pores shrinkage effect) 1

The persimmon leaf extract and persimmon leaf extract fermentation broths prepared in Preparation Examples 1 and 2 were tested for pore contraction effect using hemoglobin as a protein to replace the skin. A solution (0.05 g / 50 mL) was prepared by mixing 0.9% saline phosphate buffer (pH 7.4) with hemoglobin, 2 mL of the sample was added to 2 mL of the hemoglobin solution, followed by shaking mixing for 30 seconds. Next, centrifugation was performed at 3500 rpm for 10 minutes, and 2 ml of purified water was added to 1 ml of the supernatant, and the absorbance was measured at 407 nm. The results are shown in Table 1 below. 2 ml of sample solvent was added instead of the sample as a control.

density Precipitation of hemoglobin (%) Fermentation broth extract Control 0.22 0.21 One 20.14 14.25 2 42.95 24.68 4 89.12 51.26

As can be seen from Table 1, both persimmon leaf extract and persimmon leaf extract fermentation broth increased concentration-dependent precipitation of hemoglobin appeared to be excellent pores shrinkage effect, persimmon leaf extract fermentation broth has a better effect than persimmon leaf extract Could know.

Experimental Example  2: protein aggregation effect (pores shrinkage effect) 2

The effect of pore contraction was tested using albumin as a protein to replace the skin. After dissolving 0.3% albumin in 0.05M citric acid buffer (pH 4.5), 1 ml of each concentration sample was added to 2 ml of 0.3% albumin, and the control group was treated with Experimental Example 1 except that citric acid buffer was added instead of albumin. The absorbance was measured by the same method, and the result is shown in Table 2. The higher the absorbance value, the higher the pore shrinkage effect.

density Absorbance Fermentation broth extract Control 0.008 0.008 One 0.648 0.415 2 1.524 0.857 4 2.657 1.627

As can be seen from Table 2, the absorbance of the persimmon leaf extract fermentation broth and the persimmon leaf extract was higher than the control group, and the absorbance of the persimmon leaf extract fermentation broth was higher than that of the persimmon leaf extract. It can be seen that excellent.

Example  And Comparative example

A composition containing persimmon leaf extract fermentation solution (Example), a composition containing persimmon leaf extract (Comparative Example 1), and a composition containing nothing (Comparative Example 2) were prepared in the compositions shown in Table 3 below.

ingredient Example Comparative Example 1 Comparative Example 2 Content (% by weight) Content (% by weight) Content (% by weight) Persimmon leaf extract fermentation broth (manufacturing example 2) 2.0 - - Persimmon Leaf Extract (Preparation Example 1) - 2.0 - A Cetostearyl alcohol 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 Mikey-Crystalline 0.7 0.7 0.7 Squalane 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 Trioctanoine 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 Tocopherol Acetate 0.2 0.2 0.2 Micromethicone 3.0 3.0 3.0 BHT 0.05 0.05 0.05 B glycerin 4.0 4.0 4.0 1,3-butylene glycol 2.0 2.0 2.0 EDTA-2Na 0.05 0.05 0.05 Purified water To 100 To 100 To 100 Incense, preservative Quantity Quantity Quantity

Experimental Example  3: sebum inhibitory effect

In Example, Comparative Example 1 and Comparative Example 2, the sebum inhibitory effect on human skin was confirmed. Forty women aged 20 to 50 years old were divided into four groups, each with a test site on the forehead and applied to each group without coating, Example, Comparative Example 1 and Comparative Example 2 in the morning and evening. Thereafter, the sebum secretion of the skin was measured at 1 week intervals using a skin oil meter (Sebum meter C + K electronic GmbH, Germany). The experiment was carried out at a constant temperature and humidity conditions of 25 ℃, relative humidity 45%. For oily skin, the measured value is 220 µg / cm 2 or more, and normal skin is 100 to 220 µg / cm 2. The average value of each group is shown in Table 4 below.

Week 0 1 week 2 weeks 3 weeks 4 Weeks No coating 240 239 240 246 241 Example 241 223 213 210 207 Comparative Example 1 239 230 228 220 215 Comparative Example 2 240 240 237 243 241

As can be seen from Table 4, the sebum inhibitory effect was excellent in Example and Comparative Example 1, the effect was confirmed to be more excellent in the Example.

The following is an example of the cosmetic formulation of the present invention, but illustrates a flexible lotion, essence, shampoo and body cleanser containing the persimmon leaf extract fermentation solution of the present invention, but the cosmetic formulation of the present invention is not necessarily limited thereto.

Formulation example  1: Flexible Cosmetics

Flexible longevity containing the persimmon leaf extract fermentation broth prepared in Preparation Example 2 was prepared according to the conventional method in the composition of Table 5.

Raw material Content (% by weight) Persimmon Leaf Extract Fermentation Solution 1.00 1,3-butylene glycol 1.00 Disodium ID 0.05 Allantoin 0.10 Dipotassium glycylizate 0.05 Citrix Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 Hydrogenated Castor Oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavor a very small amount Purified water Balance

Formulation example  2: nutrition cream

Nutritional cream containing the persimmon leaf extract fermentation broth prepared in Preparation Example 2 was prepared according to the conventional method in the composition shown in Table 6.

Raw material Content (% by weight) Persimmon Leaf Extract Fermentation Solution 1.0 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extracts 3.2 Magnesium Aluminum Silicate 0.3 PEG-40 Stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Lipophilic glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlylic / Capric Triglycerides 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycylizate a very small amount Allantoin a very small amount Sodium hyaluronate a very small amount Tocopheryl acetate Quantity Triethanolamine Quantity Preservative Quantity Flavor Quantity Purified water Balance

Claims (5)

Persimmon leaf extract extracted by adding a polar solvent selected from water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof is inoculated with at least one strain selected from yeast (Saccharomyces), fungi (Aspergillus) or Lactobacillus. A cosmetic composition for reducing pores, characterized in that it contains a persimmon leaf extract fermentation broth fermented at 25 ~ 50 ℃ for 24 to 120 hours as an active ingredient. delete The cosmetic composition for reducing pores according to claim 1, wherein the persimmon leaf extract fermentation broth contains 0.01 to 30.0 wt% based on the total weight of the composition. delete delete