WO2006101119A1

WO2006101119A1 - Collagen synthesis promoter

Info

Publication number: WO2006101119A1
Application number: PCT/JP2006/305693
Authority: WO
Inventors: Masami Tachikawa; Fumito Karakida; Zenji Kawakami; Daisuke Matsuura; Hirotoshi Kanatani
Original assignee: Tsumura & Co.
Priority date: 2005-03-22
Filing date: 2006-03-22
Publication date: 2006-09-28
Also published as: JP2006265120A

Abstract

A collagen synthesis promoter capable of exhibiting a satisfactory collagen synthesis promoting activity and a high skin conditioning potency. There is provided a collagen synthesis promoter comprising as an active ingredient an extract from the fruit of myrobalan, and provided a collagen synthesis promoter comprising as an active ingredient one or two or more members selected from the group consisting of chebulagic acid, chebulinic acid, gallic acid and corilagin. The provided collagen synthesis promoter is suitable for use as a skin external preparation or skin quality improver; and can impart moistness and suppleness to the skin, can effect texture conditioning and can sustain the water retaining power of superficial skin.

Description

Specification

Collagen synthesis promoter

Technical field

TECHNICAL FIELD [0001] The present invention relates to a collagen synthesis promoter, and more specifically, a collagen synthesis promoter aimed at promoting collagen synthesis in fibroblasts, imparting freshness and firmness to the skin, and maintaining the water retaining ability of the epidermis. About.

Background art

[0002] Collagen is the main protein that forms the binding thread and weave of animals. Collagen occupies nearly 30% of the total protein of the human body. In the skin, collagen has a function of imparting freshness, thickness and thickness to the skin, retaining the water retaining ability of the epidermis, and retaining the elasticity of the skin. However, due to internal factors such as aging and external factors such as ultraviolet rays and active oxygen, the softness and moisturizing power of the skin decline, leading to aging phenomena such as wrinkles and sagging.

[0003] These are caused by a decrease in the number of cells that produce the extracellular matrix of the dermis, a decrease in cell functions such as a decrease in the cell division rate, a decrease and degeneration of collagen fibers, a decrease in subcutaneous adipose tissue, and the like. Occurs due to relaxation and loss of elasticity.

[0004] In particular, collagen is known to play an important role in the suppression and recovery of skin aging phenomenon. For the purpose of improving skin aging phenomenon, a composition containing collagen or the synthesis of collagen is known. A method of applying a composition containing a substance that promotes skin to the skin has been performed.

[0005] Ascorbic acid phosphate, retinoic acid, and the like are known as substances that promote collagen synthesis. Ascorbic acid phosphate is a type of ascorbic acid derivative that is converted to vitamin C by transdermal absorption. Vitamin C is one of the cell growth factors that induces the formation of cells by maturing the intercellular substances (extracellular matrix) with a collagen skeleton that only activates collagen synthesis. It has been clarified by an experiment of cell culture using ascorbic acid-2-phosphate Mg salt (APM) (see Non-Patent Document 1).

[0006] Retinoic acid is a derivative of vitamin A, and its physiological activity is about 300 times that of vitamin A. It is the body of biological activity of vitamin A in the body itself. In the United States, it has been approved by the FDA (Food and Drug Administration) as a therapeutic drug for skin and acne.

[0007] Further, Non-Patent Document 2 reports that an alcohol extract of leaves of milobaran (Terminalia chebula) has an effect on wound healing. In an in vivo test using rats, It significantly promoted epithelialization, and a significant increase in protein, DNA and collagen components in the granulation and weaving was observed. It has been reported that the leaf power of milobaran has been isolated from keblagic acid, kebric acid, corilagin, and gallic acid (see Non-Patent Document 3). Non-patent literature l: Eur. J. Biochem, 174, p231 (1988)

Non-Patent Document 2: Phytother.Res, 16, p227 (2002)

Non-Patent Document 3: Bull.Central Leather Res.Inst., 8, p230 (1961)

Disclosure of the invention

Problems to be solved by the invention

[0008] The method of applying collagen to the skin surface to supply collagen to the skin does not essentially improve skin function because collagen has a high molecular structure and is not absorbed by the barrier function of the epidermis. I helped. Further, collagen synthesis promoting substances such as retinoic acid are desired to be further improved rather than being sufficiently satisfactory in terms of effects.

Accordingly, an object of the present invention is to provide a collagen synthesis promoter having a sufficient collagen synthesis promoting action and a high skin conditioning effect.

Means for solving the problem

[0010] As a result of intensive studies to solve the above problems, the present inventor has found that the fruit (coconut) of Milobaran (Terminalia chebula) is high and contains a component having a collagen promoting action! /. The present invention has been completed. That is, the collagen synthesis promoter of the present invention is characterized by comprising an active ingredient of an extract of fruit of milobaran (hereinafter also referred to as “mylobaran fruit extract”). As mentioned above, it is known that the alcoholic extract of milobaran leaves has a wound healing effect and significantly increases the amount of collagen in the granulation tissue. However, the extract of milobaran fruit is effective in promoting collagen. On the other hand, it was not known to have a better effect. In addition, regarding the degree of the collagen synthesis promoting action of myrobalan leaf, the details are unknown and it is difficult to obtain. In addition, Milova None of the substances contained in the orchid leaf extract was known as to which substances contribute more to collagen synthesis.

[0011] The other collagen synthesis promoter of the present invention is one or more selected from the group consisting of chebulagic acid, chebulinic acid, gallic acid and corilagin. It is characterized by containing two or more active ingredients.

[0012] Furthermore, the collagen synthesis promoter of the present invention can be suitably used as an external preparation for skin, and can be suitably used as a skin quality improving agent for use.

The invention's effect

[0013] The collagen synthesis promoter of the present invention can effectively promote the synthesis of collagen. In addition, when the collagen synthesis promoter of the present invention is used as an external preparation for skin, it is possible to give the skin freshness, texture, texture, and to maintain the water retaining ability of the epidermis.

Brief Description of Drawings

[0014] [Fig. 1] (a) A micrograph of the skin when a topical skin preparation containing milobalan fruit cocoon kiss is used, and (b) is a micrograph of the skin when only the base is applied.

BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, preferred embodiments of the present invention will be specifically described. The collagen synthesis promoter of the present invention contains a milobaran fruit extract as an active ingredient, but a milobaran fruit extract can be obtained by a conventional method. For example, the milobaran fruit is soaked or heated under reflux with an extraction solvent. Then, it is filtered, and the resulting extract can be obtained as it is or by concentrating it. In addition, the concentrated or dried extract can be dissolved in a solvent and used again. As the extraction solvent, any solvent that is usually used for extraction can be used, and examples thereof include water and organic solvents such as ethanol, 1,3-butylene glycol, propylene glycol, acetone, methanol, and ethyl acetate. Any one of these forces can be used, or two or more can be used in any combination. The extraction conditions are not particularly limited. For example, for 1 part by weight of milobaran fruit, 2 to 100 parts by weight of solvent, and the temperature and time during extraction are 0 to 10 minutes at a temperature of L00 ° C. ~ 1 week can be. [0016] In addition, the other collagen synthesis promoter of the present invention comprises one or more active ingredients selected from the group consisting of keblagic acid, kebric acid, gallic acid and corilagin, which are components contained in the milobaran fruit extract. Preferably, keblagic acid and kevulinic acid that exhibit a high collagen synthesis promoting action and have a high content are used. Each component can be obtained by extracting and isolating plant power or synthesizing by a known method. The fruit strength of milobaran can be suitably obtained by extracting the milobaran fruit extract and then isolating it by the above method. The isolation method is not particularly limited, but it can be suitably isolated by techniques such as liquid-liquid distribution, various chromatographies, and recrystallization. Each of the components contained in the thus-obtained milobaran fruit extract and milobaran fruit extract has an excellent collagen synthesis promoting action. The collagen synthesis promoter of the present invention can be used in various fields such as pharmaceuticals, quasi drugs, foods and cosmetics.

[0017] When the collagen synthesis promoter of the present invention is used as a skin external preparation, the compounding amount of milobalan fruit extract and the components of kerobic acid, kebric acid, gallic acid and corilagin, which are the components of myrobalan fruit extract, Although it varies depending on the type, dosage form, etc., it is usually preferably 0.0001 to 5% as a dry weight and more preferably 0.001 to 0.1% as a total weight in the total amount of the external preparation. Below this range, the effect of promoting collagen synthesis is hard to be fully exerted.If the content exceeds this range, the effect cannot be expected to increase, and it is not economical. This is preferable because of problems such as dyeing with crude drugs!

[0018] The skin external preparation of the present invention includes, in addition to the above-mentioned myrobalan fruit extract, other components that are usually used in skin external preparations such as cosmetics and pharmaceuticals within the range not impairing the effects of the present invention, for example, Moisturizer, oil component, surfactant, vitamins, proteolytic enzyme, thickener, preservative, powder, antioxidant, ultraviolet absorber, emulsifier, alcohol, color material, aqueous component, water, various A skin nutrient or the like can be appropriately blended as necessary.

[0019] Examples of the humectant include polyhydric alcohols such as glycerin, propylene glycol, polyethylene glycol and 1,3 butylene glycol, sugar alcohols such as sorbit and mannitol, hyaluronate, chondroitin sulfate, chitosan and the like. Water-soluble polymer, urea, milk Examples thereof include sodium acid and pyrrolidone carboxylate.

[0020] Examples of the oil component include natural fats and oils such as soybean oil, nuka oil, apogado oil, almond oil, olive oil, cacao butter, sesame oil, persic oil, castor oil, coconut oil, mink oil, beef tallow, and lard. Hardened oils obtained by hydrogenation of these natural fats and oils, synthetic glycerides such as myristic acid glyceride, 2-ethylhexanoic acid glyceride, fats and oils such as diglyceride, jojoba oil, carnauba wax, whale wax, beeswax, lanolin, etc. Waxes, liquid paraffin, petrolatum, paraffin, microcrystalline wax, hydrocarbons such as ceresin, squalane, pristane, lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, linolenic acid Higher fatty acids such as lanolinic acid and isostearic acid, lauryl alcohol monole, Higher alcohols such as cetinoleanoreconole, stearinoreanoreconole, oleinoleanoreconole, cholesterol, 2-hexyldecanol, jojoba alcohol, cetyl octanoate, myristyl lactate, cetyl lactate, myristic acid Examples include myristyl, octyldodecyl myristate, isopropyl myristate, isopropyl palmitate, isopropyl adipate, butyl stearate, decyl oleate, cholesterol isostearate, essential oils, and silicone oils.

[0021] Examples of the surfactant include glycerin fatty acid ester, propylene glycol fatty acid ester, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, tetraoleic acid polyoxyethylene sorbite, polyoxyethylene alkyl ether, Polyoxyethylene polyoxypropylene alkyl ether, polyoxyethylene alkyl phenyl ether, polyoxyethylene fatty acid ester, polyethylene glycol fatty acid ester, polyoxyethylene castor oil, polyglycerin fatty acid ester, fatty acid monodalide, sucrose fatty acid ester, higher fatty acid al Nonionic surfactants such as strength olamide, sodium a-olefin sulfonate, sodium lauryl sulfate, sodium cetyl sulfate, poly Sodium xylethylene lauryl sulfate, sulfosuccinic acid Sodium lauryl sulfonate, linear alkylbenzene sulfonate, branched alkylbenzene sulfonate, alkyl sulfate, alkenyl sulfate, alkyl ether carboxylic acid with ethylene oxide and Z or propylene oxide Salt or alkenyl tercarboxylate, _a —sulfo fatty acid ester, amino acid type surfactant, phosphoric acid ester Anionic surfactants such as tellurium type surfactants, taurine type surfactants, amide ether sulfate type surfactants, amphoteric surfactants such as carboxybetaine type, aminocarboxylic acid, sulfobetaine type and quaternary ammo -The ability to include cationic surfactants such as um salt Nonionic surfactants are particularly preferred in terms of safety.

[0022] Vitamins include, for example, vitamin A, vitamin B, vitamin C, vitamin D, vitamin E, vitamin F, vitamin, vitamin P, vitamin U, carcin, ferulic acid, γ-oryzanol, lipo Examples include acids, orotic acid and derivatives thereof. Examples of the proteolytic enzyme include pepsin, trypsin, chymotrypsin, cathebcin, papain, bromelain, ficin, and bacteria, yeast, and mold-derived proteases.

[0023] Examples of the thickener include water-soluble polymer compounds such as carboxybule polymer, carboxymethylcellulose, polybulal alcohol, carrageenan, gelatin, xanthan gum, polyacrylate, and sodium chloride and potassium salt. An inorganic salt is mentioned. Examples of the preservative include phenoxyethanol, methyl paraben, ethyl paraben, butyparaben, sodium benzoate and the like. Examples of powders include talc, sericite, my strength, kaolin, silica, bentonite, vermiculite, zinc white, mica, mica titanium, titanium oxide, magnesium oxide, zirconium oxide, barium sulfate, bengara, iron oxide, Examples include ultramarine blue. Other ingredients include fragrances, pigments, fungicides and the like.

[0024] In addition, the dosage form of the external preparation for skin of the present invention is arbitrary. For example, it can be made into a lotion, a milk, a cream, an ointment, a dispersion, a gel, an aerosol, a nose, a bath, and the like. it can.

Example

Hereinafter, the present invention will be described in detail according to examples.

Row 1

Preparation example of mirobalan ラン kiss

To 20 g of myrobalan fruit (dried product), 20 mL of 30% ethanol was added, heated for 2 hours, and allowed to cool. After allowing to cool, filtration was performed, and the filtrate was dried under reduced pressure. As a result, 7.61 g of milobaran fruit extract could be obtained. Separately, the insoluble matter was filtered off from 50.28 g of Myrobalan fruit extract obtained by room temperature extraction with 60% acetone, and then Sephadex LH-20 (Farmasia) was removed. ), MCI gel CHP20P (Mitsubishi Chemical Co., Ltd.), cellulose, and various column chromatography using ODS gel. Gallic acid, kebulinic acid, chebulanin and corilagin were obtained.

[0026] [Table 1]

[0027] Experimental Example 2

Collagen synthesis

Human fibroblasts (Detroit551) were cultured in MEM medium containing 10% FBS, 1% NEAA, lmmol / L sodium pyruvate under conditions of 37 ° C, 5% CO-95% air.

2

It was. Next, cells were collected by trypsin treatment, adjusted to 2 × 10 ⁵ ZmL, and seeded at 100 / zL each in a 96-well microplate. Under conditions of 37 ° C, 5% CO—95% air after sputum culture

2

Replace the sample with 0.5% FBS MEM medium (150 L) containing 37. C, 5% CO—95% a

The cells were cultured for 3 days under 2 ir conditions. In addition, the samples are, as examples, myrobalan fruit extract (Example 1), keblagic acid (Example 2), gallic acid (Example 3), and kerblic acid (Example 3), which are the main components of myrobalan fruit extract. 4) and corilagin (Example 5), as a comparative example, quebrin, as a reference example, L-ascorbic acid 2-phosphate ester Mg salt (APM), retinoic acid, which is known to have a conventional ability to promote collagen synthesis, Each sample was adjusted to a concentration of 25, 50, lOOppm and used.

[0028] 90 μL of the culture supernatant after the culture was transferred to an ELISA plate, and the amount of collagen synthesis was measured by ELISA using an anti-human collagen type 1 antibody. A calibration curve using human collagen type 1 as a standard was used for quantification. The collagen production promotion rate was calculated with the value when no sample was added as 100%, and the results are shown in Table 2 below.

[0029] [Table 2] Ratio of composition amount to control mouth (%)

Sample

Sample concentration (lOOppm) Sample concentration (50ppm) 料 Type material concentration (25ppm)

Myrobalan fruit extract 657.4 ± 77.3 * 353.5 ± 106.1 * 262.4 ± 8.2 "

1

Example

Keblagic acid 755.6 ± 73.9 "517.8 ± 53.8" 263.7 ± 19.9 "*

2

Example

Gallic acid 864.6 ± 54.2 "460.0 ± 27.8" 336.6 ± 12.8 ** 3

Example

Kebric acid 1 160.7 ± 55.0 "521.7 ± 35.8" 495.0 ± 32.0 "4

Example

Collagen 236.8 ± 31.5 '253.1 ± 75.6' 173.1 ± 10.7 * 5

Comparative example

Kebranin 1 1 1.9 ± 9.1 58.1 ± 28.2 140.6 ± 18.4 1

Reference example

AP 307.7 ± 27.7 "436.6 ± 58.4 ** 307.0 ± 24.1" 1

Reference example

Retinoic acid 292.8 ± 22.2 "123.8 ± 36.6 234.1 ± 14.5"

2

π = 5 ** p 0.005 * p 0.05

[0030] From Table 2 above, collagen synthesis was significantly promoted in all of the examples, and the extract of fruit of miloparan (Example 1), keblagic acid (Example 2), gallic acid (Example 3) In addition, it can be seen that kebulinic acid (Example 4) has a higher collagen synthesis promoting effect than the reference example.

[0031] Experimental Example 3

Preparation example of external preparation for skin (cream preparation)

Using the milobaran fruit obtained in Experimental Example 1 and following the formulation (% by weight) shown in Table 3 below, in accordance with the production example below, a cream formulation containing 0.5% milobaran fruit extract (hereinafter referred to as “Milobalan formulation”). A cream ", u) was prepared.

[0032] First, liquid paraffin, isopropyl palmitate, stearic acid, cetanol, glyceryl monostearate, dimethylpolysiloxane, and some esters of paraoxybenzoate were mixed and heated. Next, the remaining paraoxybenzoic acid ester, 1,3-butyldaryl, disodium edetate, triethanolamine, and purified water were mixed and heated, and added to the previous mixture to emulsify. After cooling, a cream was prepared by adding phenoxyethanol and myrobalan fruit extract.

[0033] [Table 3] Ingredient name Myrobalan cream combination Base only

Liquid paraffin 10.0 10.0

Isopropyl palmitate 3.0 3.0

Stearic acid 4.5 4.5

Cetanol 3.0 3.0

Glycerol monostearate 3.5 3.5

Dimethylpolysiloxane 1.0 1.0

/ Laoxybenzoic acid ester 0.3 0.3

1,3-butyl glycol 5.0 5.0

Nedarium edetate 0.05 0.05

Triethanolamine 0.8 0.8

Phenoxyethanol 0.2 0.2

Purified water 68.15 68.65

Myrobalan fruit extract 0.5-

100.0 100.0

[0034] Experimental Example 4

Preparation example of human bath

In accordance with the formulation (% by weight) shown in Table 4 below, a powder bath containing milobaran fruit extract was prepared by uniformly mixing all raw materials.

[0035] [Table 4]

[0036] According to the formulation (% by weight) shown in Table 5 below: 1 により, Phase A and Phase B were heated to 70 ° C to completely dissolve, and after Phase A was added to Phase B, cooled to 30 ° C A liquid bath containing a myobalan fruit extract was prepared.

[0037] [Table 5] Raw material name Compounding amount (weight)

(Phase A)

Liquid paraffin 30.0

Comenu oil 10.0

POE Setilje 2.5

Mono / << Sorbitan Luminate 2.5

Phenoxyethanol 0.3

Fragrance 0.5

(Phase B)

Purified water 53.7

Myloparan fruit extract 0.3

Methyl paraoxybenzoate 0.2

100.0

[0038] Experimental Example 5

Preparation example of fossil water

In accordance with the formulation (% by weight) shown in Table 6 below, the alcohol phase is added to the aqueous phase to prepare a stock solution, the stock solution is put into a can, and filled with a gas such as LPG or butane. Aerosol product) was prepared.

[0039] [Table 6]

[0040] Formulation of Table 7 in accordance with (wt ^_0/0), the alcohol phase was添Ka卩the aqueous phase, pH 5 by solubilizing. 5 Mirobaran fruit extract combination weakly acidic I匕粧water (transparent type) Was prepared.

[0041] [Table 7] Raw material name Compounding (wt%)

(Water phase)

1.3-Butylene glycol 5.0

Glycerin 5.0

Sodium pyrrolidonecarboxylate 0.5

Sodium hyaluronate 0.001

Polyvinylpyrrolidone 0.1

Quenic acid

Purified water remaining

(Alcohol phase)

Ethanol 10.0

POE oleyl ether 1.0

Oleil alcohol 0.1

Tokovic oxalate: I: Roll 0.01

Preservative 0.1

Perfume

Myrobalan fruit extract 5.0

100.0

According [0042] Formulation of Table 8 (wt ^_0/0), the alcohol phase was添Ka卩the aqueous phase was prepared more pH 7. 5 Mirobaran fruit extract formulation lotion of (cloudy type) to be emulsified.

[0043] [Table 8]

According to the formulation shown in Table 9 (weight ^_0/0), After Ka溶I匕by adding alcohol phase in an aqueous phase, by adding a powder phase PH6. Mirobaran fruit extract combination of 3-layer type 2 A lotion (separate type) was prepared.

[0045] [Table 9] Raw material name Compounding amount

(Water phase)

1.2-Hexanediol 3.0

1.3-Butylene glycol 5.0

Multi! ^ One 0.1

Sodium hyaluronate 0.05

Xanthan gum 0.01

Malic acid

Purified water remaining 5

(Alcohol phase)

Ethanol 20.0

POE sorbitan monolaurate 0.1

Skullan 3.0

Hohono oil 0.5

Menthol 0.02

Camphor 0.005

Preservative 0.1

Fragrance

Myrobalan fruit extract 1.0

(Powder phase)

Iron oxide (Bengara) 0.02

Zinc oxide 0.2

Kaolin 1.0

Talc 2.0

100.0

[0046] Experiment 6

Example of cream preparation

According to the formulation (% by weight) shown in Table 10 below, the oil phase and the aqueous phase were each heated to 70 ° C for complete dissolution. The oil phase was added to the aqueous phase to emulsify, and after cooling, the added phase was added to prepare a body cream containing a myloparan fruit extract with pH 6.1.

[0047] [Table 10]

Raw material name Compounding amount (weight)

(Oil phase)

Cetanol 0.5

Vaseline 2.0

Skulun 10.0

Self-emulsifying glyceryl monostearate 2.5

POE sorbitan monostearate 1.5

Jojoba oil 1.0

Pantoyl ether ether 0.05

Preservative 0.1

(Water phase)

Propylene glycol 1.0

1,2-Hexanediol 1.0

Glycerin 0.5

Montmorillonite 2.0

Bentonite 0.5

Carboxymethyl cell mouthpiece 0.1

Preservative 0.2

Purified water remaining

(Additional phase)

Perfume

Tartaric acid 0.1

Myloparan fruit extract 0.0001

100.0

[0048] According to the formulation (% by weight) shown in Table 11 below, the oil phase and the aqueous phase were each heated to 70 ° C for complete dissolution. An oil phase was added to the aqueous phase to emulsify, and after cooling, an additional phase was added to prepare a face cream with a myobalan fruit extract having a pH of 7.5.

[0049] [Table 11]

Raw material name Compounding (weight%)

(Oil phase)

Stearic acid 2.0

Stearyl alcohol 3.0

Deodorized Lanolin 2.0

Olive oil 15.0

Glycerol monostearate2.0

POE oleyl ether 2.0

Tri (cabril 'cabric acid) glycerin 5.0

Tokov acetate: c-roll 0.05

Retinol palmitate 0.05

Preservative 0.1

卜 Coferre 0.05

(Water phase)

1,3-Butylene glycol 3.0

Sorbi! Le 1.0

Diglycerin 1.0

Xanthan gum 0.25

Carboxyvinyl polymer 0.1

Potassium hydroxide 0.1

Preservative 0.2

Purified water remaining

(Additional phase)

Perfume

Myloparan fruit extract 0.001

100.0

[0050] According to the formulation (% by weight) shown in Table 12 below, the oil phase and the aqueous phase were each heated to 70 ° C to be completely dissolved. An oil phase was added to the aqueous phase to emulsify, and after cooling, a finely pulverized powder phase and an additional phase were added to prepare a sunscreen tarm containing SPF20 milobaran fruit extract.

[0051] [Table 12]

Raw material name Compounding amount (heavy

(Oil phase)

Stearic acid 1.0

Setanoir 3.0

Shea fat 4.0

Olive oil 10.0

Monostearic acid POE glycerin 2.0

POE oleyl ether 1.0

2-Ethylhexanoic acid cetyl 5.0

Tocopherol acetate 0.05

Retinol palmitate 0.05

Preservative 0.1

Natural vitamin E 0.05

2-Methoxyhexyl p-methoxycinnamate 1.0

Oxybenzone 0.5

(Water phase)

1,2-pentanediol 3.0

Sorbi! ^ One 2.0

Glycerin 0.5

Xanthan gum 0.8

Preservative 0.2

Purified water remaining

(Powder phase)

Titanium oxide 12.0

Zinc oxide 0.5

Bengala 0.001

Titanium oxide · Keyed anhydride composite 2.0

(Additional phase)

Perfume

Myrobalan fruit extract 0.01

100.0 Experimental Example 7

Example of smoked tonic

In accordance with the formulation shown in Table 13 below ( ^zero weight), first, dl-a tocopherol acetate, isopropylmethylphenol, pantothel alcohol, glycyrrhetinic acid, ethanol (99.5%), octyldodecyl myristate, 2-ethylhensanic acid Octyl, fragrance, and menthol were mixed uniformly. Next, sempri extract (extract), carrot extract (extract), 1,3-butylene glycol, dipropylene glycol, sodium ascorbate phosphate, sodium edetate, polyoxyethylene hydrogenated castor oil ( 50E.O), polyoxyethylene hydrogenated castor oil (20E.O), myrobalan fruit extract, and purified water were mixed uniformly. Finally, both were mixed, filtered and filled to prepare a liquid type tonic containing milobaran fruit extract. [0053] [Table 13]

[0054] According to the formulation (% by weight) shown in Table 14 below, first, dl-a tocolol acetate, isopropylmethylphenol, pantol alcohol, ethanol (99.5%), flavor, and menthol were mixed uniformly. did. Next, sempri extract (extract), carrot extract (extract), dipropylene glycol, 3-methyl-1,3-butanediol, asconolevic acid ester sodium phosphate, edetate tetrasodium, polyoxyethylene polyoxypropylene Glycol, decaglyceryl monolaurate, myrobalan fruit extract, and purified water were mixed uniformly. Next, both were mixed and filtered to obtain a stock solution. Finally, the stock solution and propellant were matched to the filling formulation and filled into cans to prepare an aerosol-type tonic containing milobaran fruit extract.

[0055] [Table 14] Raw material name Compounding amount

(Undiluted solution)

Succinic acid d 卜 a-卜 Cofero ^ (Le 0.1

Assembly extract (extract) 1.0

Isopropylmethylphenol 0.1

Pantothenyl alcohol 0.5

Carrot extract (extract) 1.0

Ethanol (99.5%) 10.0

Dipropylene glycol 3.0

3-methyl-1.3-butanediol 5.0

Ascorbic acid sodium phosphate 0.1

Edetic acid 4sodium 0.1

Polyoxyethylene polyoxypropylene glycol 3.0

Decaglyceryl monolaurate 0.5

Fragrance 0.3

Menthol 0.3

Myrobalan fruit extract 0.5

Purified water remaining

100.0

(Propellant)

DME 70.0

PG 30.0

100.0

(Filling prescription)

Stock solution 80.0

Propellant 20.0

100.0

[0056] Experimental Example 8

As a topical skin preparation; commercial example

Milobaran fruit extract obtained in Experimental Example 3 0.5% cream formulation and as a comparative example, only the base was applied to the left and right hand (back of hand) of a 30-year-old woman three times a day, about 30% In addition, the skin gloss, firmness, softness, crispness, dullness, texture and wrinkle before and after application for 1 month were evaluated according to the following evaluation criteria.

[0057] (Evaluation criteria)

Shiny, firm, flexible: “No (1), not very much (2), neither can be said (3), some (4)

There is (5) "

A feeling of rustling: “Crispy (1), Slightly rustling (2), Both, Ena (3), Slightly moist (4), Moist (5)”

Dullness: “There is (1), some (2), both! Fearless, (3), Much! /, (4), ¾ ヽ (5) J Kime: “Ah ヽ (1), Ah, Ah, (2), both of them, Ena, (3), Slightly fine!

Shio: “Essential (1), Slightly outstanding (2), both are Ena (3), Less prominent (4), Inconspicuous (5)”

The evaluation results are shown in Table 15 below.

[Table 15]

[0059] From Table 15 above, it is clear that the extract of milobaran fruit is glossy, firm, soft, crisp, dull, textured, and wrinkled It was very effective to show good effects in all items.

[0060] Fig. 1 shows the results of micrographs after application for 1 month. (A) shows the case where a topical skin preparation containing milobalan fruit juice is applied, and (b) shows the case where only the base is applied. From the photograph, it can be seen that the texture is better when using a topical skin preparation containing a milobaran fruit extract compared to the base alone. In addition, moisturizing power and elasticity improved, and dullness disappeared.

Claims

The scope of the claims

[1] A collagen synthesis promoter characterized by comprising an extract of myrobalan fruit as an active ingredient.

[2] The collagen synthesis promoter according to claim 1, which is an external preparation for skin.

[3] The collagen synthesis promoter according to claim 1, which is a skin quality improving agent.

[4] A collagen synthesis promoter characterized by comprising one or more selected active ingredients as keblagic acid, kebric acid, gallic acid, and collilaginity.

[5] The collagen synthesis promoter according to claim 4, which is an external preparation for skin.

[6] The collagen synthesis promoter according to claim 4, which is a skin quality improving agent.