JP3382146B2 - External preparation for skin - Google Patents

Description

Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an effect of synergistically enhancing moisturizing action and dermal fibroblast activating action, and preventing dermal fibroblasts from being damaged by ultraviolet rays. The present invention relates to a skin external preparation which is effective for preventing or improving skin aging symptoms such as generation of wrinkles and decreased skin elasticity, and also has an anti-inflammatory action and a wound healing promoting action. For more information,
The present invention relates to a skin external preparation comprising a mixture of a fruiting body extract of Agaricus blazei Murill and a sugar isomerized by the action of an alkali hydroxide solution (isomerized sugar mixture). [0002] Skin aging symptoms such as wrinkles, blemishes, and reduced skin elasticity caused by aging and external stresses such as ultraviolet rays are accompanied by a decrease in the function of fibroblasts in the skin dermis, a decrease in matrix fibers, or the like. Decomposition is an important factor.
Therefore, in order to obtain an external preparation for preventing aging that has the effect of preventing and improving skin aging, search and blending of components having the activity of activating or promoting proliferation of fibroblasts have been attempted. [0003] For example, loquat extract (Japanese Patent Publication No. 5-1720)
No. 6), α-hydroxyacetic acid (Japanese Unexamined Patent Publication No.
22, sterol esters of α-hydroxy acids (JP-A-8-104632), 6-benzylaminopurine (JP-A-7-2333037), specific ribonucleases (JP-A-7-309778), L-lysyl-L-glycyl-L-histidine (JP-A-7-31619)
No. 2), milk-derived fibroblast growth factor (Japanese Unexamined Patent Publication No.
No. 119867), oxidized coenzyme A (JP-A-8-175961) and the like. [0004] However, some of the above-mentioned components having an effect of activating dermal fibroblasts have insufficient action effects or poor stability, and may be contained in a base for external preparation for skin. In some cases, significant amounts had to be included in order to obtain an effective effect. In addition, those which have undesirable side effects or irritation, which adversely affect the stability of the preparation, those which are difficult to mix with external preparations in terms of odor or color, difficult to maintain a certain action and quality There were many things. Accordingly, in the present invention, the moisturizing action and the dermal fibroblast activating action are synergistically enhanced, and aging of the skin such as generation of wrinkles and spots and reduction of skin elasticity is achieved. An object of the present invention was to obtain an external preparation for skin that exerts an excellent effect in preventing or improving symptoms. Means for Solving the Problems [0006] The present inventors have developed a fruiting body extract of Agaricus blazei Murill,
It has been found that a high dermal fibroblast activating action can be obtained (Japanese Patent Application No. 9-267936). This time, it was found that the combined use of this Agaricus mushroom fruit body extract and the isomerized saccharide mixture activates the skin physiological function and synergistically enhances the moisturizing effect and the fibroblast activating effect. By adding the composition to the external preparation for skin, it is possible to obtain an external preparation for skin which can exhibit an excellent effect of preventing or improving aging symptoms of the skin such as generation of wrinkles and spots and reduction of skin elasticity. Came to a solution. An embodiment of the present invention will be described. The agaricus mushroom ( Agaricu) used in the present invention
s blazei Murill) is a kind of agaric genus of the basidiomycete agaricaceae agaricaceae, and is also referred to as kawariharatake or himematsutake, and is deposited with the National Institute of Advanced Industrial Science and Technology, National Institute of Advanced Industrial Science and Technology. In the present invention, when an extract is obtained from the fruit body of Agaricus mushroom, either a raw fruit body or a dried fruit body may be used. As the extraction solvent from the fruiting body, a polar solvent is preferably used. For example, water, alcohols such as ethanol, methanol, isopropanol, isobutanol, n-hexanol, methylamyl alcohol, 2-ethylbutanol, n-octyl alcohol, glycerin, ethylene glycol, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether Solvent or polyhydric alcohol such as propylene glycol, propylene glycol monomethyl ether, propylene glycol monoethyl ether, triethylene glycol, 1,3-butylene glycol, hexylene glycol or derivatives thereof, or two or more solvents Can be used. Further, an inorganic salt, a surfactant and the like may be added to a polar solvent. Among these polar solvents, one solvent selected from ethanol, methanol, 1,3-butylene glycol and water or a mixed solvent of two or more thereof, and a solvent obtained by adding an inorganic salt or a surfactant to these solvents Is preferably used. [0010] Further, as the extraction method, a method of extracting by impregnation at room temperature while being cooled or heated, a method of extraction using a distillation method such as steam distillation, and a method of directly pressing raw Agaricus mushroom fruit bodies. Examples of the squeezing method to obtain the extract,
These methods are used alone or in combination of two or more. The ratio of the Agaricus mushroom fruit body to the solvent at the time of extraction is not particularly limited, but the solvent is 0.5 to 1000 times the weight of Agaricus mushroom fruit body 1, especially in terms of extraction operation and efficiency. Is preferably 0.5 to 100 times by weight. The extraction temperature is conveniently in the range of 5 ° C. to the boiling point of the solvent at normal pressure, and the extraction time varies depending on the extraction temperature and the like, but is preferably in the range of 2 hours to 2 weeks. The extract of Agaricus mushroom fruit body obtained as described above can be used as it is, but it can be used for fractionation, deodorization, decolorization, concentration, etc. within a range that does not impair the effects of the present invention. A purification operation may be added for use. These extracts, their fractions, and purified products can be dried by removing the solvent therefrom, and can be further dissolved or suspended in a solvent such as purified water or an emulsion. It can be added to a skin external preparation in a form. The amount of the Agaricus mushroom fruit body extract to be added to the external preparation for skin is desirably in the concentration range of 0.0001 to 5% by weight in view of its effect, odor and color tone when added. The saccharide mixture isomerized by the action of an alkali hydroxide solution (isomerized saccharide mixture) used in the present invention is obtained by isomerizing the saccharide with an alkali hydroxide solution such as sodium hydroxide or potassium hydroxide. The reaction product mixture is preferably used, in particular, a product obtained by isomerizing glucose or lactose, or a mixture thereof.
These can be produced by the method described in JP-B-48-1504 and the like. The content in the skin external preparation is suitably about 0.0001 to 3.0% by weight. The external preparation for skin according to the present invention includes oils and fats, waxes, hydrocarbons, fatty acids, lower alcohols, higher alcohols, polyhydric alcohols, esters, and the like which are usually used as the base for external preparation. Surfactants, water-soluble polymers and the like can be contained. Further, other skin cell activators, anti-inflammatory agents, active oxygen scavengers, whitening agents, humectants, ultraviolet absorbers, preservatives and fungicides, fragrances and the like can be contained. The external preparation for skin according to the present invention can be provided in the form of lotions, emulsions, gels, creams, ointments and the like. Also, skin cosmetics such as lotions, emulsions, creams, beauty essences, massage agents, packs, etc., makeup cosmetics such as makeup base lotions, makeup base creams, liquid or creamy foundations, hand creams, etc. It can also be provided as a body cosmetic such as leg cream and body lotion. EXAMPLES Examples of the production of the Agaricus mushroom fruit body extract and the isomerized saccharide mixture used in the Examples of the present invention are shown below. [Preparation Example 1] Agaricus mushroom fruit body extract The dried Agaricus mushroom fruit body was pulverized, and 10% by weight of 50% by weight ethanol was added as an extraction solvent, immersed at room temperature for 3 days, and filtered. The filtrate was used as an Agaricus mushroom fruit body extract. [Production Example 2] 1 kg of the isomerized saccharide mixture D-glucose was dissolved in 2,000 ml of purified water.
10% (w / v)% sodium hydroxide solution 1 while stirring
Add 0 ml and leave sealed at 21-23 ° C. Thereafter, 10 ml of 10% (w / v) sodium hydroxide solution is added so as to always maintain pH 9 or more, and a total of 6% is added.
After 0 ml was added, lactic acid was added to adjust the pH to 6, the reaction was terminated, and the solution was designated as solution A. At this stage, the weight ratio of L-glucose to the converted glucose (mainly fructose) is about 6: 4. On the other hand, 1 kg of lactose was similarly dissolved in 2,000 ml of purified water, and
10 ml of (w / v)% sodium hydroxide solution are added,
Leave tightly sealed at 21-23 ° C. Then, as in the case of solution A, p
A 10 (w / v)% sodium hydroxide solution is added in 10 ml portions so as to maintain H9 or more, and after adding a total of 140 ml, lactic acid is added to adjust the pH to 6, thereby terminating the reaction to obtain a solution B. At this stage, the weight ratio of the isomerized lactose to the reduced lactose (mainly galactose) is 5: 5. The liquid A and the liquid B are mixed at a weight ratio of 19: 1 to obtain an isomerized saccharide mixture. Table 1 shows the composition of this mixture. [Table 1] In the present invention, the synergistic activation of fibroblasts obtained by using the Agaricus mushroom fruit body extract in combination with the isomerized saccharide mixture will be described below. [Activity of dermal fibroblast metabolism activation] Human-derived dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well, and shown in Table 2 after 24 hours. 37 ° C. in Dulbecco's minimum essential medium supplemented with 1.0% by volume fetal calf serum containing each of the production examples
For 48 hours. Then, 2- (4,5-dimethyl-2-thiazolyl) -3,5-diphenyltetrazolium bromide (MT
T) was replaced with the above-mentioned medium containing 0.4 mg / ml.
After culturing at 7 ° C. for 2 hours, formazan generated by opening the tetrazolium ring was extracted with 2-propanol and 550
Measured by absorbance in nm. The system cultured in only Dulbecco's minimum essential medium containing 1.0% by volume of fetal bovine serum was used as a control, and the system cultured in Dulbecco's minimum essential medium containing 5.0% by volume of fetal bovine serum was used as a positive control. The results are shown in Table 2 by the activation index in which the absorbance in the control was expressed as 100.0%. [Table 2] As a result, as shown in Table 2, operation example 1
Then, in comparison with the operation example 2 using the production example 1 alone,
When a small amount of Production Example 2 was used in combination, a fibroblast activating action of about twice was observed, indicating that the fibroblast activating action was synergistically improved. Next, examples prepared using the above-mentioned production examples will be shown, and the present invention will be described in more detail. [Example 1, Comparative Examples 1 to 3] O / W emulsifying cosmetic liquid Using each of the production examples shown in Table 3, O / W was formulated according to the following formulation.
A W-emulsion type serum was prepared. (Prescription) (1) Squalane 5.0 (% by weight) (2) White petrolatum 2.0 (3) Beeswax 0.5 (4) Sorbitan sesquioleate 0.8 (5) Polyoxyethylene oleyl ether (20EO) 1.2 (6) Methyl paraoxybenzoate 0.1 (7) Propylene glycol 5.0 (8) Purified water Amount to make total amount 100 (9) 1.0% by weight aqueous solution of carboxyvinyl polymer 20.0 (10) Hydroxidation Potassium 0.1 (11) Ethanol 5.0 (12) Production Example Amount shown in Table 3 (13) Fragrance 0.2 Production method: Mix oil phase components (1) to (5) and heat to 75 ° C Dissolve and homogenize. On the other hand, the aqueous phase components of (6) to (8) are mixed,
Dissolve and heat to 75 ° C., add oil phase components and pre-emulsify. After adding (9), the mixture is emulsified uniformly with a homomixer, and (10) is added to adjust the pH. After cooling, at 40 ° C (1
1) to (13) are added, mixed and homogenized. [Table 3] Using Example 1 and Comparative Examples 1 to 3, the effect of preventing wrinkles caused by ultraviolet rays was evaluated. The effect of preventing wrinkles was determined by grouping five hairless mice as one group, and in each group, the example and the comparative example were each 1 day / day.
And applied to the back of the umbilical cord for 1 J / cm ² / week long wavelength ultraviolet (U
VA) was irradiated for 50 weeks, the occurrence of wrinkles in the hairless mouse was observed, and scored according to the criteria shown in Table 4. At this time, a group to which only purified water was applied was used as a control. The results were calculated as the average value of each group, and are shown in Table 5 in relation to the number of days of UVA irradiation. [Table 4] [Table 5] As shown in Table 5, the control group of Comparative Example 2 containing only the isomerized saccharide mixture of Production Example 2 had the same U-type as the control group of Comparative Example 3 containing no control and Production Example.
The occurrence of slight wrinkles was observed when the number of days of VA irradiation exceeded 40 weeks, and the occurrence of moderate wrinkles was observed after 50 weeks. On the other hand, in the application group of Comparative Example 1 containing only Production Example 1, the generation of wrinkles was remarkably suppressed to the extent that fine wrinkles were observed after 50 weeks. On the other hand, Example 1
In Comparative Example 1, although only a 1/5 amount of the production example of Comparative Example 1 was contained, a wrinkle generation suppressing effect superior to that of Comparative Example 1 was recognized, and a wrinkle generation suppressing effect was not recognized. It was shown that the combined use of a small amount of the compound of Example 1 synergistically improves the effect of suppressing the generation of wrinkles in Production Example 1. Subsequently, Example 1 of the present invention and Comparative Examples 1 to
For Comparative Example 3, the anti-inflammatory effect and the effect of promoting wound healing were evaluated. Five groups of mice each having artificial inflammation or wound formation were used.
5 g was applied twice a day for 7 days, and on the 7th day, the condition of the inflammatory site and the wound site was observed. The anti-inflammatory effect was evaluated in three stages: "effective", "slightly effective", "ineffective", and the effect of promoting wound healing in three stages: "complete healing", "almost healing", and "incomplete healing". Table 6 shows the number of obtained mice. [Table 6] As is clear from Table 6, the anti-inflammatory effect and the effect of promoting wound healing were confirmed to be effective in four or more mice in the group to which the Examples of the present invention were applied. As a result, the same effect as Comparative Example 1 in which the Agaricus mushroom fruit body extract of Production Example 1 was blended in a 5-fold amount alone was exhibited. Next, a practical use test for 6 months was conducted for Example 1 of the present invention and Comparative Examples 1 to 3. As panelists, women in their 40s and 60s who have skin symptoms such as remarkable wrinkles or decreased elasticity, and women in their 20s and 50s who show remarkable skin roughening symptoms, 20 people in each group And In the use test, each group was allowed to use each of the examples and comparative examples in a blind manner, and the skin condition was observed before the start of the use test and after the end of the use test. Each improvement of wrinkles and skin elasticity was evaluated in three stages of “improvement”, “slight improvement”, and “no change”. Table 7 shows the number of panelists who obtained each evaluation. In addition,
About the degree of wrinkles, by photography and replica,
The skin elasticity was measured and evaluated with a cute meter. Regarding the rough skin, the skin condition was scored according to the criteria shown in Table 8, and the results before and after the end of the use test were compared in Table 9 based on the average value of 20 persons. [Table 7] As shown in Table 7, no wrinkles and skin elasticity were not improved in the group using the examples of the present invention, and the improvement tendency was recognized in all the panelists. An improvement effect slightly superior to Comparative Example 1 containing 5 times the fruiting body extract was observed. On the other hand, Comparative Example 2 containing only the isomerized saccharide mixture of Production Example 2
, Wrinkles and elasticity tended to be improved, but there were 5 and 7 panelists who answered that there was no change. [Table 8] [Table 9] As shown in Table 9, in the group using the example of the present invention, improvement tendency of rough skin was observed in all panelists. On the other hand, in Comparative Example 1 containing 5 times the amount of Agaricus mushroom fruit body extract of Production Example 1, although the tendency to improve skin roughness was observed, the combined use with the isomerized saccharide mixture of Production Example 2 improved skin roughness. The effect was shown to be significantly improved. A moisture retention test was performed using Example 1 and Comparative Examples 1 to 3. The moisturizing property was determined by applying the example to the inside of the forearm in a constant temperature and humidity room at a temperature of 20 ° C. and a relative humidity of 50%.
1 ml / cm ^{2 was} applied, and after 30 minutes, the water content of the epidermis was measured using a high-frequency impedance meter (SKINCON 20 manufactured by IBS).
0) and evaluated. As a control, the water content of the skin of the portion where the Examples and Comparative Examples were not applied was measured, and the difference was defined as the increase in the water content of the skin. 10 for each sample
The average value of the name panelists was calculated and the results are shown in Table 10. [Table 10] As a result, in Example 1, 45 parts from the uncoated portion
The water content of the epidermis is increased by μS or more, and is about 2.5 times that of Comparative Example 1 containing only the Agaricus mushroom fruit body extract, and about twice that of Comparative Example 4 that contains only the isomerized sugar mixture. The amount increased, and it was shown that the moisturizing effect was synergistically improved by using the Agaricus mushroom fruit body extract and the isomerized saccharide mixture in combination. In Example 1 of the present invention, precipitation, separation, agglomeration, unpleasant odor,
No change in the state of the preparation such as discoloration was observed. In addition, in each group used in Examples, there was no paneler showing a skin irritating reaction or a skin sensitizing reaction. Next, the formulation of another embodiment of the present invention will be described. Example 2 Skin Lotion (1) Ethanol 10.0 (wt%) (2) Hydroxyethylcellulose 1.0 (3) Agaricus mushroom fruit body extract (Production Example 1) 0.2 (4) Isomerized saccharide mixture (Production Example 2) 0.05 (5) Methyl paraoxybenzoate 0.1 (6) Purified water 88.65 Production method: Mix (1) to (6) to make uniform. Example 3 Skin Emulsion (1) Stearic Acid 0.2 (% by weight) (2) Cetanol 1.5 (3) Vaseline 3.0 (4) Liquid Paraffin 7.0 (5) Polyoxy Ethylene (10EO) monooleate 1.5 (6) Tocopherol acetate 0.5 (7) Glycerin 5.0 (8) Methyl paraoxybenzoate 0.1 (9) Triethanolamine 1.0 (10) Purified water 79.5 (11) Agaricus mushroom fruit body extract (Production Example 1) 0.5 (12) Isomerized sugar mixture (Production Example 2) 0.2 Production method: mixing oil phase components (1) to (6) Dissolve uniformly by heating and keep at 70 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and heated to be uniform, and the temperature is set to 70 ° C. The oil phase component is gradually added to the aqueous phase component while stirring to emulsify, and after cooling, (11) and (12) are added and mixed at 40 ° C. Example 4 Skin Gel (1) Purified Water 88.4 (% by weight) (2) Carboxyvinyl Polymer 0.5 (3) Dipropylene Glycol 10.0 (4) Methyl Paraoxybenzoate 0 0.1 (5) Potassium hydroxide 0.1 (6) Agaricus mushroom fruit body extract (Production Example 1) 0.8 (7) Isomerized saccharide mixture (Production Example 2) 0.1 Production method: (1) After uniformly dissolving (2), (4) is dissolved and added to (3), and then (5) is added to increase the viscosity, (6) and
(7) is added. Example 5 Skin Cream (1) Beeswax 6.0 (% by weight) (2) Cetanol 5.0 (3) Reduced Lanolin 8.0 (4) Squalane 27.5 (5) Glyceryl fatty acid ester 4.0 (6) Lipophilic glyceryl monostearate 2.0 (7) Polyoxyethylene (20EO) sorbitan monolaurate 5.0 (8) Propylene glycol 5.0 (9) Methyl paraoxybenzoate 1 (10) Purified water 36.7 (11) Agaricus mushroom fruit body extract (Production Example 1) 0.5 (12) Isomerized saccharide mixture (Production Example 2) 0.2 Production method: (1) to (7) And dissolve and heat to 75 ° C. On the other hand, the aqueous phase components (8) to (10) are mixed and dissolved and heated to 75 ° C. Next, the oil phase component is added to the water phase component and pre-emulsified, then uniformly emulsified by a homomixer, and after cooling, (11) and (12) are added and mixed at 40 ° C. Example 6 Oil-in-water emulsion ointment (1) White petrolatum 25.0 (% by weight) (2) Stearyl alcohol 25.0 (3) Glycerin 12.0 (4) Sodium lauryl sulfate 1.0 (5) Methyl paraoxybenzoate 0.1 (6) Purified water 35.8 (7) Agaricus mushroom fruit body extract (Production Example 1) 1.0 (8) Isomerized sugar mixture (Production Example 2) 0.1 Production method: The oil phase components (1) to (4) are mixed and dissolved to make uniform, and heated to 75 ° C. On the other hand, dissolve (5) in (6)
Heat to 5 ° C, add the oil phase component to this and emulsify,
After cooling, add (7) and (8) at 40 ° C and mix. Example 7 Toner (1) Ethanol 10.0 (% by weight) (2) 1,3-butylene glycol 5.0 (3) Agaricus mushroom fruit body extract (Production Example 1) 0.1 (4) Isomerized sugar mixture (Production Example 2) 0.05 (5) Fragrance 0.1 (6) Purified water 84.75 Production method: (1) to (5) are sequentially added to (6) to be uniform Mix and dissolve. [Example 8] Emollient cream (water-in-oil type) (1) Liquid paraffin 30.0 (% by weight) (2) Microcrystalline wax 2.0 (3) Vaseline 5.0 (4) Diglyceryl olein Acid ester 5.0 (5) Sodium L-glutamate 1.6 (6) L-serine 0.4 (7) Propylene glycol 3.0 (8) Methyl parahydroxybenzoate 0.1 (9) Purified water 52.55 (10) Fragrance 0.1 (11) Agaricus mushroom fruit body extract (Production Example 1) 0.2 (12) Isomerized sugar mixture (Production Example 2) 0.05 Production method: (5), (6) Dissolve in part of 9) to 50 ° C,
Add slowly to (4) heated to 50 ° C. with stirring.
This was previously mixed and dissolved by heating at 70 ° C. (1)-
(3) is uniformly dispersed, and (7) and (8) are dissolved in the remainder of (9) and heated to 70 ° C. and added thereto with stirring.
Emulsify with a homomixer. After cooling, at 40 ° C (10) ~
Add (12) and mix. Example 9 Makeup Base Cream (1) Stearic acid 12.0 (% by weight) (2) Cetanol 2.0 (3) Glyceryl tri-2-ethylhexanoate 2.5 (4) Self-emulsification Type glyceryl monostearate 2.0 (5) Propylene glycol 10.0 (6) Potassium hydroxide 0.3 (7) Purified water 69.0 (8) Titanium oxide 1.0 (9) Bengala 0.1 ( 10) Yellow iron oxide 0.4 (11) Fragrance 0.1 (12) Agaricus mushroom fruit body extract (Production Example 1) 0.5 (13) Isomerized sugar mixture (Production Example 2) 0.1 Production method: ( The oil phase components (1) to (4) are mixed and heated to 75 ° C. to be uniform. On the other hand, the aqueous phase components (5) to (7)
The mixture is heated and dissolved at 5 ° C. to make the mixture uniform, and the pigments (8) to (10) are added thereto and uniformly dispersed by a homomixer. The oil phase component is added to the aqueous phase component, emulsified by a homomixer, cooled, and (11) to (13) are added and mixed at 40 ° C. Example 10 Emulsion Foundation (1) Stearic acid 2.0 (% by weight) (2) Squalane 5.0 (3) Octyldodecyl myristate 5.0 (4) Cetanol 1.0 (5) Decaglyceryl monoisopalmitate 9.0 (6) 1,3-butylene glycol 6.0 (7) Potassium hydroxide 0.1 (8) Methyl parahydroxybenzoate 0.1 (9) Purified water 53.4 ( 10) Titanium oxide 9.0 (11) Talc 7.4 (12) Bengala 0.5 (13) Yellow iron oxide 1.1 (14) Black iron oxide 0.1 (15) Fragrance 0.1 (16) Agaricus Mushroom fruit body extract (Production Example 1) 0.15 (17) Isomerized sugar mixture (Production Example 2) 0.05 Production method: mix oil phase components (1) to (5) and heat to 75 ° C And make it uniform. On the other hand, the aqueous phase components (6) to (9)
The mixture is heated to 5 ° C. and dissolved to make the mixture uniform, and the pigments (10) to (14) are added thereto and uniformly dispersed by a homomixer. The oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified by a homomixer, then cooled, and (15) to (17) are added and mixed at 40 ° C. Example 11 Hand cream (1) Cetanol 4.0 (% by weight) (2) Vaseline 2.0 (3) Liquid paraffin 10.0 (4) Glyceryl monostearate 1.5 (5) Polyoxyethylene (60EO) Glyceryl isostearate 2.5 (6) Tocopherol acetate 0.5 (7) Glycerin 20.0 (8) Methyl parahydroxybenzoate 0.1 (9) Purified water 59.1 (10) Agaricus Mushroom fruit body extract (Production Example 1) 0.2 (11) Isomerized sugar mixture (Production Example 2) 0.1 Production method: Mix and dissolve oil phase components (1) to (6) to 75 ° C Heat. On the other hand, the aqueous phase components (7) to (9) are mixed and dissolved and heated to 75 ° C. Next, the oil phase component is added to the water phase component and pre-emulsified, then uniformly emulsified and cooled with a homomixer, and (10) and (11) are added and mixed at 40 ° C. As described in detail above, the external preparation for skin using the Agaricus mushroom fruit body extract in combination with the isomerized saccharide mixture synergistically improves the dermal fibroblast activation and moisturizing effects.
It has the effect of protecting the dermal fibroblasts from damage by ultraviolet rays, is effective in preventing or improving skin aging symptoms such as wrinkles and reduced skin elasticity, and has an anti-inflammatory effect and a promoting effect on wound healing. The stability and safety were also good.

────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁷ identification code FI A61P 17/16 A61P 17/16 (58) Investigated field (Int.Cl. ⁷ , DB name) A61K ^7/ 00-7/50 JICST file (JOIS) CA (STN)

Claims (1)

(57) [Claims] [Claim 1] Agaricus blazei Murill
An external preparation for skin, comprising a mixture of a fruiting body extract and a sugar isomerized by the action of an alkali hydroxide solution.