JP3280903B2 - External preparation for skin - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention is characterized by containing sericin and its hydrolyzate, and yeast extract, yeast hydrolyzate, and extract of yeast hydrolyzate (hereinafter collectively referred to as yeast extract). More specifically, a skin external preparation has an effect of promoting the production of collagen in dermal fibroblasts, and prevents or ameliorates aging symptoms of the skin such as generation of skin wrinkles and decreased elasticity, rough skin, dullness, and sagging. It is intended to provide a skin external preparation which is effective for skin and can also exert a moisturizing effect.

[0002]

2. Description of the Related Art One of the skin aging phenomena is the occurrence of wrinkles and a decrease in elasticity. This is thought to be caused by the disappearance of collagen from the dermis with aging and the thinning of the dermis. Accordingly, many cosmetics containing collagen and cosmetics containing a collagen production promoting substance have been developed.

[0003] Collagen in the dermis is produced by dermal fibroblasts and degraded by collagenase produced by dermal fibroblasts. Therefore, cosmetics have been proposed that contain a collagenase inhibitor that inhibits the activity of collagenase, thereby suppressing the degradation of collagen and suppressing the occurrence of wrinkles and a decrease in elasticity.

[0004] However, conventional cosmetics containing collagen have problems in percutaneous absorption and stability, and have not fundamentally improved the amount of collagen in the dermis. In addition, a sufficient anti-aging effect has not been obtained in cosmetics containing a collagen production promoter or a collagenase inhibitor, and more effective anti-aging agents have been desired.

[0005]

DISCLOSURE OF THE INVENTION The present invention exerts an effect of promoting the production of collagen in dermal fibroblasts, and causes aging of the skin such as generation of wrinkles and a decrease in elasticity, rough skin, dullness, and sagging. It is an object of the present invention to obtain an external preparation for skin that is effective in preventing or improving skin and that is excellent in safety.

[0006]

The present applicants have reported that sericin and its hydrolyzate exert a sufficient collagen production promoting effect on dermal fibroblasts, and that a skin external preparation containing the same exhibits wrinkling. In addition, it has been found that it suppresses a decrease in elasticity and elasticity, and is excellent in both safety and stability (Japanese Patent Application No. 9-50896). This time, by combining this sericin and its hydrolyzate with a yeast extract in an external preparation for skin, the production of collagen in dermal fibroblasts is synergistically improved, and the generation of skin wrinkles and elasticity The present inventors have found that it is effective in preventing or improving skin aging symptoms such as reduction, rough skin, dullness, and sagging, and that it is excellent in safety, and has solved the above-mentioned problems.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, sericin and its hydrolyzate are prepared by directly converting sericin contained in silkworm cocoons or raw silk.
Alternatively, those eluted by partial hydrolysis in an alkaline aqueous solution or by enzymatic treatment can be used. Also,
The eluate was adjusted to pH 3 to 5 with an organic or inorganic acid, or mixed with a water-soluble organic solvent such as methanol, ethanol, dioxane, etc. to precipitate a sericin hydrolyzate, filtered, dried and powdered. Those can also be used. Further, sericin and its hydrolyzate used in the present invention have an average molecular weight of about 10,000 to 20,000.
And those containing 20 to 40 mol% of serine are particularly preferable.

[0008] Sericin and its hydrolyzate used in the present invention are easily water-soluble, have excellent miscibility with ordinary skin external preparation components, maintain stable properties as an aqueous solution, and have an irritant to the skin. No sensitization and excellent safety. Such sericin and its hydrolyzate are blended as an anti-aging active ingredient in skin preparations such as pharmaceuticals, quasi-drugs, and cosmetics.

In the present invention, yeasts for obtaining yeast extract include yeasts belonging to the genus Saccharomyces , such as brewer's yeast, sake yeast, wine yeast, and baker's yeast.

In the case of using a decomposition product of yeast by self-digestion, 10 to 100 times by weight of purified water is added to the wet cells after washing, and the cells are added at 35 to 45 ° C. for about 24 to 72 hours. After digestion, if necessary, a substance obtained by filtration, freeze-drying or concentration under reduced pressure can be used.

In the case of using a yeast hydrolyzate degraded by a protease, 10 to 100 times by weight of purified water is added to the washed wet cells, and the protease is added in an amount of 20 to 100 kg per 1 kg of the wet cells. About 500,000 units are added, and the reaction is carried out at about the optimum temperature of the enzyme for 12 to 48 hours. Next, after inactivating the enzyme, an appropriate amount of a polar solvent may be added, if necessary, followed by centrifugation or the like, followed by filtration, freeze-drying or concentration under reduced pressure.

When an acid hydrolyzate of yeast is used, an acid such as hydrochloric acid is added to the wet cells after washing, and the acid is added to the cells.
Hydrolysis with occasional stirring at 60 ° C. for 3 to 8 hours, neutralization with an alkali such as sodium hydroxide,
After filtration, if necessary, those obtained by freeze-drying or concentration under reduced pressure can be used.

[0013] These yeast digests may be used as a yeast extract as they are, or an extract of the yeast digest obtained by extracting from the yeast digests by the following method may be used as the yeast extract.

In order to obtain an extract from yeast, the cells after culturing are collected, washed with purified water or the like to obtain wet cells, and the wet cells are destroyed as they are or by high frequency. Used.

[0015] Water,
Ethanol, methanol, isopropanol, isobutanol, n-hexanol, methyl amyl alcohol, 2-
Monohydric alcohols such as ethylbutanol and n-octyl alcohol, glycerin, ethylene glycol, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, propylene glycol, propylene glycol monomethyl ether, propylene glycol monoethyl ether, triethylene glycol, 1, 3-
One or more polar solvents such as polyhydric alcohols such as butylene glycol and hexylene glycol or derivatives thereof are selected and added as an extraction solvent to perform extraction. The extraction solvent is not particularly limited as long as it is a polar solvent as described above, but from the viewpoint of safety and stability when blended in a skin external preparation, purified water, ethanol, 1,3-butylene glycol, glycerin, propylene It is preferable to use glycol alone or in combination of two or more.

Examples of the extraction method include a method of immersion extraction at room temperature, in a cooled or heated state, and a method of extraction using a distillation method such as steam distillation. These methods may be used alone or in combination of two or more. Extraction is performed by combining the above.

The ratio of the wet cells to the solvent at the time of extraction is not particularly limited.
~ 1000 times by weight, especially 0.5 ~ in terms of extraction operation and efficiency
100 weight times is preferred. The extraction temperature is 5 at normal pressure.
It is convenient to keep the temperature in the range from about ° C. to the boiling point of the solvent or lower, and the extraction time varies depending on the extraction temperature and the like, but is preferably in the range of 2 hours to 2 weeks.

The extract obtained by using a polar solvent from the yeast cells and the yeast degradation product in this manner can be used as it is, but it can be deodorized, decolorized and concentrated within a range not to lose the effects of the present invention. Or by fractionation using column chromatography or the like. These extracts can be made into a dry product by removing the solvent from them, and further formulated in a form solubilized or suspended in a solvent such as purified water or alcohol, or in the form of an emulsion in an external preparation for skin. can do.

[0019] These sericin and its hydrolyzate,
Effective for preventing or improving yeast aging symptoms of yeast extract
It is desirable that the compounding amount in the external preparation for skin should be in the concentration range of 0.0001 to 5% by weight in consideration of the effect and the scent and color tone when added. 0.00%
If it is less than 01% by weight, a sufficient effect cannot be obtained,
There is no need to mix too much.

In the present invention , the skin senescence containing both the above-mentioned sericin and its hydrolyzate and yeast extract is used.
It provides an external preparation for skin that is effective in preventing or improving aging symptoms.
The preparation may be in the form of a lotion, emulsion, cream, ointment or the like. Furthermore, flexible lotion,
Lotions such as astringent lotion and lotion for washing, creams such as emollient cream, moisture cream, massage cream, cleansing cream, makeup cream, emollient emulsion, moisture emulsion,
Effective in preventing or improving skin aging symptoms in various formulations, such as emulsions such as nourishing emulsions and cleansing emulsions, packs such as jelly packs, peel-off packs, wash-off packs, powder packs, serums and facial cleansers. What
It can also be provided as a cosmetic .

In the present invention, further, other anti-aging components, whitening components, moisturizing components, anti-inflammatory agents, ultraviolet absorbers, etc.
Other active ingredients can also be used in combination, and can be provided as cosmetics such as whitening cosmetics, sunscreen cosmetics, skin protection cosmetics and the like, or quasi-drugs.

[0022]

EXAMPLES Examples of the production of sericin hydrolyzate and yeast extract used in Examples of the present invention will be described first.

[Production Example 1] Sericin hydrolyzate A silkworm cocoon is treated with a bacterial alkaline protease derived from Bacillus licheniformis at pH 8.0 and 55 ° C for 1 hour to elute the sericin hydrolyzate. The eluate was concentrated under reduced pressure, and twice the volume of ethanol was added to precipitate a sericin hydrolyzate, which was separated by filtration and dried to obtain a sericin hydrolyzate. The sericin hydrolyzate had an average molecular weight of about 13,000 and contained 35 mol% of serine.

[Production Example 2] Yeast extract 1 Beer yeast is suspended in a 30% by weight ethanol solution, and this suspension is sufficiently homogenized with a homomixer, followed by centrifugation and filtration to collect a filtrate.

[Production Example 3] Yeast extract 2 Sake yeast is suspended in purified water and autolyzed at 45 ° C for 48 hours. Next, this was freeze-dried, and a 30% by weight of 1,3-butylene glycol solution was added thereto and sufficiently stirred and extracted.
Centrifuge and filter, and collect the filtrate.

[Production Example 4] Yeast extract 3 Wine yeast is suspended in purified water, a protease (Actinase A, manufactured by Kaken Pharmaceutical Co., Ltd.) is added, and the mixture is reacted at 37 ° C for 24 hours. Then, after inactivating the enzyme, propylene glycol is added to a final concentration of 30% by weight, centrifuged, filtered, and the filtrate is collected.

[Preparation Example 5] Yeast extract 4 Beer yeast is suspended in 3% hydrochloric acid and hydrolyzed at a temperature of 50 ° C for 6 hours with occasional stirring. After disassembly,
Neutralize with sodium hydroxide, filter with a membrane filter, concentrate under reduced pressure and desalinate.

The effect of promoting the production of collagen when sericin hydrolyzate and yeast extract were used in combination was examined using cultured human dermal fibroblasts. Human dermal fibroblasts were cultured in Dulbecco's minimum essential medium supplemented with fetal calf serum, and after 24 hours, the medium was replaced with the above-mentioned medium supplemented with each of the production examples shown in Table 1, and the culture was continued for 7 days. Procollagen in the culture supernatant can be converted to a commercial kit (Takara Procollag
en Type I C-Peptide EIAKit) and quantified by enzyme immunoassay (ELISA). At that time, the same culture was performed without adding a sericin hydrolyzate and yeast extract as a control, and the procollagen production rate (%) was calculated, with the procollagen production amount in the control being 100%. Procollagen is a precursor of collagen, and is converted into collagen after procollagen is secreted from inside cells.

[0029]

[Table 1]

The results of measuring the production rate of procollagen are shown in Table 1. As is clear from Table 1, the sericin hydrolyzate was obtained by simultaneously adding the yeast extract of Production Examples 2 to 5 having no collagen production promoting effect and sericin hydrolyzate and culturing fibroblasts. The procollagen production-promoting effect of the compound was synergistically improved, and a dramatic increase in procollagen production was observed. No cytotoxicity to fibroblasts was observed in the concentration range in which this experiment was performed.

Examples 1 to 4 and Comparative Examples 1 to 6
W-Emulsified Essence The sericin hydrolyzate and yeast extract were blended in the amounts shown in Table 2 to prepare an O / W-emulsified serum. (Prescription) (1) Squalane 5.0 (% by weight) (2) White petrolatum 2.0 (3) Beeswax 0.5 (4) Sorbitan sesquioleate 0.8 (5) Polyoxyethylene oleyl ether (20EO) 1.2 (6) Methyl parahydroxybenzoate 0.1 (7) Propylene glycol 5.0 (8) Purified water Amount based on the total amount of 100 (9) 1.0% by weight aqueous solution of carboxyvinyl polymer 20.0 (10) Hydroxidation Potassium 0.1 (11) Sericin hydrolyzate and yeast extract Amount shown in Table 2 (12) Purified water 1.0 Production method: Mix oil phase components (1) to (5) and heat to 75 ° C to dissolve , Make uniform. On the other hand, the aqueous phase components of (6) to (8) are mixed,
Dissolve and heat to 75 ° C. and pre-emulsify by gradually adding the oil phase component. After adding (9), the mixture is emulsified uniformly with a homomixer, and (10) is added to adjust the pH. After cooling 4
Dissolve (11) in (12) at 0 ° C., add, mix and homogenize.

[0032]

[Table 2]

Using the above Examples 1 to 4 and Comparative Examples 1 to 6, use tests were performed. First, a method for evaluating each effect will be described.

[Wrinkle improvement effect] The wrinkle improvement effect was evaluated by an actual use test for 6 months. As panelists, women in their 40s to 60s who had skin symptoms such as remarkable wrinkles were used, and 20 people were included in each group. The use test was conducted by using each of the groups in Examples 1 to 4 and Comparative Examples 1 to 6 with blinds. The skin condition was observed before the use test and after the use test, and the wrinkle improvement was evaluated in three stages of “improvement”, “slight improvement”, and “no change”, and judged according to the following criteria.

(Judgment) ：: The proportion of the subject showing improvement and slight improvement is 80% or more ○: The proportion of the subject showing improvement and slight improvement is 50% or more and less than 80% Δ: The subject is improved and slightly improved : 30% or more and less than 50% ×: The rate of improvement of the subject and slightly improvement of less than 30%

[Effect of Improving Elasticity] Twenty subjects suffering from a decrease in elasticity of the skin were taken as a group, and Examples 1 to 4 and Comparative Examples 1 to 6 were applied to the face twice a day for 3 months. I went to use. The viscoelasticity of the skin before and after use is cu
meter SEM575 (Courage & Kh
azaka, Germany), and the elasticity improvement effect was obtained by dividing the difference between the maximum height of the skin by suction and the minimum height of the skin when released after suction by the maximum height of the skin by suction. Values were scored and evaluated by the change in score before and after use. In addition, all the scores of the test subjects before use were 3 or less. The results are shown in Table 3.

[0037] (Judgment) ：: The percentage of subjects whose scores improved by 1 or more before and after use was 80% or more ：: The percentage of subjects whose scores improved by 1 or more before and after use was 50% or more and less than 80% △: The score was 1 before and after use The proportion of subjects who improved above was 30% or more and less than 50% ×: The proportion of subjects whose scores improved by 1 or more before and after use was less than 30%

[Effect of Improving Skin Roughness] A group of 20 male panels was coated with a 10% by weight aqueous solution of sodium lauryl sulfate as a surfactant for several days on several parts of the forearm.
Induced rough skin. Then, Example 1 was applied to this rough skin site.
~ Example 4 and Comparative Examples 1 to 6 were applied twice a day, and 5 days after the start of application, the state of the skin surface was observed by a replica method, and a portion to which nothing was applied after induction of skin roughness was applied. In comparison, the improvement of the rough skin was evaluated by the following scores. In addition, the score before the application of the Examples or Comparative Examples was all 2 or less at the site where the rough skin was induced.

(Skin condition score) Score 1: Elimination of skin sulcus and crusts and exfoliation of a wide range of stratum corneum are observed. : Skin ridges are recognized but flat and ridges are indistinct. 4: Skin ridges and ridges are clear. 5: Skin ridges and ridges are clear and regular. (Judgment) ◎: Score 3 80% or more of the subjects evaluated above ○: 50% or more of the subjects evaluated to score 3 or more 80
%: 30% or more of the subjects evaluated as score 3 or more.
Less than 30%: Less than 30% of subjects evaluated as score 3 or more

[Effect of Improving Dullness] Twenty subjects suffering from dullness of the skin were grouped into Examples 1 to 4 and Comparative Example 1.
Comparative Example 6 was applied to the face twice a day for 3 months, and the effect of improving dullness was evaluated according to the following criteria. The results are shown in Table 3.

(Evaluation Criteria) Significant effect: almost no dullness was felt Effective: fairly dullness was slightly sensed Effective: dullness was slightly felt ineffective Invalid: no change was felt (judgment) ：: The percentage of subjects who show significant, effective, and slightly effective is 8
0% or more ：: The proportion of subjects who show significant, effective, and somewhat effective is 5
0% or more and less than 80% △: The proportion of subjects showing significant, effective, and somewhat effective is 3
0% or more and less than 50% ×: The ratio of subjects showing significant, effective, and slightly effective is 3
Less than 0%

[Evaluation of sagging effect] A group consisting of 20 subjects suffering from sagging skin was used in Examples 1 to 4 and Comparative Example 1.
Comparative Example 6 was applied to the face twice a day for three months, and the sag improving effect was evaluated according to the following criteria. The results are shown in Table 3.

(Evaluation criteria) Significant effect: The sag was considerably improved. Effective: The sag was slightly improved. Ineffective: No change was felt. (Judgment) ：: The ratio of the subject showing the significant effect and the effect was 80%. Greater than or equal to Good: The proportion of subjects showing remarkable efficacy and efficacy is 50% or more and 80 or more.
Less than%: The percentage of the subjects showing remarkable and effective effects is 30% or more and 50% or less.
%: Less than 30% of subjects show significant and effective

[Moisturizing Effect] The moisturizing effect was measured on the inner part of the forearm in a constant temperature and humidity room at a temperature of 20 ° C. and a relative humidity of 50%.
0.01 ml / c from Example 4 and Comparative Examples 1 to 6
After applying m ² , the water content of the skin 30 minutes after the application was measured using a high-frequency impedance meter (Skicon 200, manufactured by IBS). As a control, the water content of the epidermis was measured in a portion where the Examples and Comparative Examples were not applied, and the difference was determined based on the following criteria.

(Judgment) ：: Water content increased by 40 μS or more ：: Water content increased by 25 μS or more and less than 40 μS Δ: Water content increased by 10 μS or more and less than 25 μS ×: No increase in water content was observed or increased by less than 10 μS

[0046]

[Table 3]

As shown in Table 3, the combined use of the sericin hydrolyzate and the yeast extract provided an excellent combination of wrinkle improving effect, elasticity improving effect, skin roughness improving effect, dullness improving effect, sagging improving effect and moisturizing effect. A serum was obtained. On the other hand, Comparative Example 1, in which only the sericin hydrolyzate was blended, showed an improvement tendency in wrinkle improvement effect, elasticity improvement effect, and skin roughness improvement effect, but did not show any dullness improvement effect. In Comparative Examples 2 to 5 containing only the yeast extract, wrinkles,
In each of the items of elasticity, rough skin, and sagging, less than 50% of the subjects showed an effective effect.

In the above use tests, none of the groups using any of the examples reported any skin irritation such as pain and itchiness or skin symptoms such as allergic reaction. In addition, deterioration of the emulsified state, sedimentation of the components,
No change in the state of the preparation such as alteration was observed.

Next, the formulation of another embodiment of the present invention will be described.
In Examples 7, 9, 9, 10 and 11, among the examples shown below, the sericin hydrolyzate shown in Production Examples was dissolved in purified water to obtain a 10% by weight aqueous solution. One used. [Example 5] Liquid skin external preparation (1) Glycerin 5.0 (wt%) (2) Propylene glycol 4.0 (3) Ethanol 10.0 (4) Sericin hydrolyzate 0.05 (5) Yeast extract 10.5 (6) Methyl paraoxybenzoate 0.1 (7) Purified water 80.35 Production method: Dissolve (6) in (3), add to (7), and add (1), (2),
(4) and (5) are sequentially added, mixed and homogenized.

Example 6 Lotion (1) 1,3-butylene glycol 3.0 (% by weight) (2) Sorbitol 2.0 (3) Ethanol 10.0 (4) 1% by weight aqueous solution of carboxyvinyl polymer 10.0 (5) Sericin hydrolyzate 0.07 (6) Yeast extract 2 0.5 (7) Methyl parahydroxybenzoate 0.1 (8) Fragrance 0.1 (9) Purified water 74.23 Production method: ( 7) and (8) are dissolved in (3) and added to (9), and (1),
After (2), (5), and (6) are added and mixed sequentially, (4) is added, and the mixture is mixed and homogenized.

Example 7 O / W Emulsion Ointment (1) White Vaseline 25.0 (% by weight) (2) Stearyl Alcohol 15.0 (3) Sodium Lauryl Sulfate 1.0 (4) Paraoxybenzoic Acid Butyl 0.1 (5) Purified water 57.9 (6) Sericin hydrolyzate 10% by weight aqueous solution 0.5 (7) Yeast extract 30.5 Manufacturing method: Mix oil phase components (1) to (4) Then heat to 75 ° C to dissolve and homogenize. The oil phase component is added to (5) heated to 75 ° C. to emulsify, and after cooling, (6) and (7) are sequentially added, mixed and homogenized at 40 ° C.

[Example 8] W / O emulsified cream (1) Beeswax 3.0 (wt%) (2) Adsorbed and purified lanolin 10.0 (3) Squalane 30.0 (4) Solid paraffin 2.0 ( 5) Microcrystalline wax 5.0 (6) Hexyldecyl adipate 10.0 (7) Sorbitan sesquioleate 3.5 (8) Polyoxyethylene (50EO) hydrogenated castor oil 1.0 (9) 1,3- Butylene glycol 5.0 (10) Purified water 29.7 (11) Methyl paraoxybenzoate 0.2 (12) Sericin hydrolyzate 0.1 (13) Yeast extract 40.5 Production method: (1) to (8) ) Is mixed and heated to 75 ° C. to dissolve and homogenize. On the other hand, the aqueous phase components of (9) to (13) are mixed,
Dissolve and heat to 75 ° C., add to the above oil phase components and emulsify uniformly with a homomixer.

Example 9 Makeup Base Cream (1) Stearic acid 12.0 (% by weight) (2) Cetanol 2.0 (3) Glyceryl tri-2-ethylhexanoate 2.5 (4) Self-emulsification Type glyceryl monostearate 2.0 (5) Propylene glycol 10.0 (6) Potassium hydroxide 0.3 (7) Purified water 68.4 (8) Titanium oxide 1.0 (9) Bengala 0.1 ( 10) Yellow iron oxide 0.4 (11) Fragrance 0.1 (12) Sericin hydrolyzate 10% by weight aqueous solution 0.7 (13) Yeast extract 10.5 Production method: oil phase of (1) to (4) The ingredients are mixed and heated to 75 ° C. to homogeneity. On the other hand, the aqueous phase components (5) to (7)
The mixture is heated and dissolved at 5 ° C. to make the mixture uniform, and the pigments (8) to (10) are added thereto and uniformly dispersed by a homomixer. The oil phase component is added to the aqueous phase component, emulsified by a homomixer, cooled, and (11) to (13) are added and mixed at 40 ° C.

Example 10 Emulsion Foundation (1) Stearic acid 2.0 (% by weight) (2) Squalane 5.0 (3) Octyldodecyl myristate 5.0 (4) Cetanol 1.0 (5) Decaglyceryl monoisopalmitate 9.0 (6) 1,3-butylene glycol 6.0 (7) Potassium hydroxide 0.1 (8) Methyl parahydroxybenzoate 0.1 (9) Purified water 52.3 ( 10) Titanium oxide 9.0 (11) Talc 7.4 (12) Bengala 0.5 (13) Yellow iron oxide 1.1 (14) Black iron oxide 0.1 (15) Fragrance 0.1 (16) Sericin Hydrolyzate 10% by weight aqueous solution 0.8 (17) Yeast extract 20.5 Production method: The oil phase components (1) to (5) are mixed and heated to 75 ° C. to make uniform. On the other hand, the aqueous phase components (6) to (9)
The mixture is heated to 5 ° C. and dissolved to make the mixture uniform, and the pigments (10) to (14) are added thereto and uniformly dispersed by a homomixer. The oil phase component is added to the aqueous phase component, uniformly emulsified by a homomixer, cooled, and (15) to (17) are sequentially added and mixed at 40 ° C.

Example 11 Hand Cream (1) Cetanol 4.0 (% by weight) (2) Vaseline 2.0 (3) Liquid paraffin 10.0 (4) Glyceryl monostearate 1.5 (5) Polyoxyethylene (60EO) glyceryl isostearate 2.5 (6) Tocopherol acetate 0.2 (7) Glycerin 20.0 (8) Methyl parahydroxybenzoate 0.1 (9) Purified water 58.2 (10) Sericin Hydrolyzate 10% by weight aqueous solution 1.0 (11) Yeast extract 30.5 Production method: Mix and dissolve oil phase components (1) to (6) and heat to 75 ° C. On the other hand, the aqueous phase components (7) to (9) are mixed and dissolved and heated to 75 ° C. Then, the oil phase component is added to the water phase component and pre-emulsified, then uniformly emulsified and cooled with a homomixer, and (10) and (11) are sequentially added and mixed at 40 ° C.

[0056]

As described in detail above, by containing sericin and a sericin hydrolyzate in combination with a yeast extract in the present invention, the collagen production promoting effect is synergistically improved, the wrinkle improving effect and the elasticity are improved. Skin that is effective in preventing or improving aging symptoms of the skin, which is excellent in improving effect, skin roughening effect, dullness improving effect, sagging improving effect and moisturizing effect
A skin preparation was obtained.

──────────────────────────────────────────────────続 き Continuation of front page (56) References JP-A-9-71520 (JP, A) JP-A-6-219936 (JP, A) JP-A-9-124438 (JP, A) JP-A-58-58 26809 (JP, A) JP-A-62-36308 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) A61K 7/00 A61K 7/48 A61K 35/72

Claims (7)

(57) [Claims] Claims: 1. A collagen in dermal fibroblasts containing one or more selected from sericin and its hydrolyzate and an extract obtained from yeast using a polar solvent.
An external preparation for skin for promoting genogen . 2. A dermis containing one or more selected from sericin and its hydrolyzate and a yeast hydrolyzate
An external preparation for skin for promoting collagen production in fibroblasts . 3. A method for treating dermal fibroblasts, comprising one or more selected from sericin and hydrolysates thereof and an extract obtained from a yeast hydrolyzate using a polar solvent.
Skin preparation for promoting collagen production . 4. The yeast digest is a yeast digest obtained by autolyzing yeast.
A dermal fibroblast according to claim 2 or claim 3.
Skin preparation for promoting collagen production . 5. The dermal fiber according to claim 2, wherein the yeast digest is a yeast digest obtained by digesting yeast with a protease.
An external preparation for skin for promoting collagen production in blast cells . 6. The dermal fibroblast according to claim 2, wherein the yeast digest is a digest obtained by hydrolyzing yeast with an acid .
An external preparation for skin for promoting collagen production . 7. The method according to claim 7, wherein the yeast is Saccharomyc.
es ), characterized in that it is a yeast belonging to
Collagen in dermal fibroblasts according to claim 6
An external preparation for skin to promote production .