KR20150137692A - Cosmetic composition comtaining Lethariella cladonioides extracts - Google Patents
Cosmetic composition comtaining Lethariella cladonioides extracts Download PDFInfo
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- KR20150137692A KR20150137692A KR1020140065996A KR20140065996A KR20150137692A KR 20150137692 A KR20150137692 A KR 20150137692A KR 1020140065996 A KR1020140065996 A KR 1020140065996A KR 20140065996 A KR20140065996 A KR 20140065996A KR 20150137692 A KR20150137692 A KR 20150137692A
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- mushroom
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- extract
- skin
- fermented
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/10—Bryophyta (mosses)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Birds (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Dermatology (AREA)
- Chemical & Material Sciences (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Cosmetics (AREA)
Abstract
Description
본 발명은 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물을 유효성분으로 포함하는 화장료 조성물 및/또는 피부외용제에 관한 것이다.
The present invention relates to a cosmetic composition and / or an external preparation for skin comprising as an active ingredient, an extract of Hongyuyong tea, a fermented Hongdong tea extract or a mixture thereof.
피부는 외부 환경으로부터 인체를 보호하고, 내부의 수분 및 유용 성분이 밖으로 유출되는 것을 막아주는 장벽 기능, 체온조절, 배설 등 다양한 생리적 기능을 담당하고 있는 중요한 기관이다. 그러나, 다음과 같은 이유 등에 의해 피부 세포의 활성이 저하되고 피부에 기미, 주근깨 및 검버섯 등의 변화가 생기며 피부가 칙칙하고 거칠게 변하는 노화 현상이 일어나게 된다. Skin is an important body that is responsible for various physiological functions such as barrier function, body temperature control, excretion, etc., which protects the human body from the external environment and prevents the internal moisture and useful components from flowing out. However, due to the following reasons, the activity of the skin cells is lowered, and the skin is changed to spots, freckles and black blotches, and the skin becomes dull and rough.
첫째, 피부의 진피층에 존재하는 콜라겐은 피부의 기계적 견고성, 결합조직의 저항력, 조직의 결합력, 세포접착 의 지탱 등으로 피부의 구성성분에 중요한 기능을 한다. 이러한 콜라겐은 스트레스, 고령화 또는 자외선 조사에 의한 광 노화에 의하여 감소되어, 피부 조직이 느슨해지며 탄력을 상실하게 된다. First, the collagen in the dermal layer of the skin plays an important role in the constituents of the skin due to the mechanical rigidity of the skin, the resistance of the connective tissue, the binding force of the tissue, and the support of the cell adhesion. Such collagen is reduced by photoaging due to stress, aging or ultraviolet irradiation, and the skin tissue is loosened and the elasticity is lost.
둘째, 스트레스 또는 자외선 조사와 같은 외부 요인에 의해 염증 반응이 일어나거나 노화에 의해 면역 세포들의 활성이 감소하여 피부의 면역 기능 조절이 정상적이지 못하여 피부 노화가 촉진된다. Second, inflammation reaction occurs by external factors such as stress or ultraviolet ray irradiation, or activation of immune cells is reduced by aging, and skin aging is promoted because immune function regulation is not normal.
셋째, 표피의 세포 기능이 저하되어 신진대사 작용이 원활하지 못하여, 각질 탈락이 잘 일어나지 않아 각질이 과하게 축적된 상태에서 자외선을 받으면 탄력이 저하되어 주름의 발생을 초래하게 된다. Third, the cellular function of the epidermis is deteriorated, the metabolism is not smooth, and the exfoliation does not occur well, and when the ultraviolet light is received in the state where the keratin is overly accumulated, the elasticity is decreased and wrinkles are generated.
넷째, 표피에 피지막이 형성되지 않을 경우 수분과 피지 분비가 감소하고 피부가 건조해 지면서 주름이 형성된다. Fourth, if no epidermis is formed on the epidermis, moisture and sebum secretion decrease, and the skin becomes dry and wrinkles are formed.
다섯째, 피하지방에서 생성된 산소 라디칼이 세포막과 세포핵을 파괴하여 혈액순환을 저하시킴으로써 피부의 영양과 수분이 부족하게 되어 피부 노화가 촉진된다. Fifth, oxygen radicals generated from subcutaneous fat break down cell membranes and nuclei to lower blood circulation, and skin nutrition and moisture become insufficient and skin aging is promoted.
상기와 같은 피부 주름을 개선시키고 피부 노화를 지연시키기 위하여 다양한 연구가 진행되고 있으며, 고령화 사회로의 진입에 따라 젊고 탄력 있는 피부를 갖고자 하는 소비자의 욕구가 증대되고 2000 년 7 월 화장품법의 시행됨에 따라 피부 노화를 지연시키고 피부 보습을 개선시키는 화장품에 대한 연구 및 개발이 더욱 활발해졌다. 이러한 기능의 화장품들은, 피부에 독성이나 자극을 주지 않기 위하여 천연 물질을 사용하기도 한다. Various researches have been conducted to improve the wrinkles of the skin and delay the aging of the skin. As the aging society has entered into the aging society, the desire of consumers who want to have young and elastic skin has increased, and the Cosmetic Act of July 2000 Research and development of cosmetics that delay skin aging and improve skin moisturization have become more active. Cosmetics of this nature also use natural materials to avoid toxicity or irritation to the skin.
그 중 고산지대에서 주로 자생하는 이끼류인 홍설차는 홍설차(Snow tea, Lethariella Cladonioides)는 금사차라고도 불리우며, 비타민과 미네랄, 아미노산 등의 성분을 함유하고 있고, 현재까지 고혈압, 비만 효과 등이 알려져 있으며(비특허문헌 1), 피부 개선과 피부 미용을 목적으로 사용한 전례는 없다. Snow tea (Lethariella Cladonioides), also known as gold tea, contains vitamins, minerals and amino acids, and has been known for its high blood pressure and obesity effects (Non-Patent Document 1), there are no cases in which skin is used for skin improvement and skin care.
이에, 본 발명자들은 종래의 화장료 조성물에 비해 항산화 효과, 피부 보습 및 주름 개선 효과가 뛰어나며, 그 효과가 오래 지속되는 생약재를 선별하던 중 홍설차 추출물이 피부에 도포할 경우 항산화 효과뿐만 아니라 피부 보습, 주름 개선 효과가 개선됨을 확인함으로써 본 발명을 완성하게 되었다.Accordingly, the present inventors have found that when extracting the herbal medicine which is excellent in antioxidative effect, skin moisturizing and wrinkle reducing effect compared with the conventional cosmetic composition and selecting the long-lasting medicinal herbs, And the wrinkle-improving effect is improved, thereby completing the present invention.
따라서, 본 발명의 목적은 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물을 유효성분으로 포함하는 화장료 조성물을 제공하는데 있다.Accordingly, an object of the present invention is to provide a cosmetic composition comprising as an active ingredient, an extract of Hongkong family, a fermented Hongkong family extract or a mixture thereof.
또한, 본 발명의 다른 목적은 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물을 유효성분으로 포함하는 피부외용제를 제공하는데 있다.
It is another object of the present invention to provide an external preparation for skin comprising as an active ingredient an extract of Siberian thunbergii, a fermented Siberian tusk extract or a mixture thereof.
상기 과제를 해결하기 위한 수단으로서, 본 발명은 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물을 유효성분으로 포함하는 화장료 조성물을 제공한다.
As means for solving the above-mentioned problems, the present invention provides a cosmetic composition comprising as an active ingredient, an extract of Saururus thunbergii, a fermented Saururus thunbergii extract or a mixture thereof.
상기 과제를 해결하기 위한 다른 수단으로서, 본 발명은 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물을 유효성분으로 포함하는 피부외용제를 제공한다.
As another means for solving the above-mentioned problems, the present invention provides an external preparation for skin comprising as an active ingredient an extract of Siberian thunbergii, a fermented Siberian larva extract or a mixture thereof.
본 발명에 따른 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물은 항산화, 보습 및/또는 주름 개선 효과가 우수하면서도 피부에 알러지 반응이나 자극을 유발하지 않아 사람의 피부에 안전하므로, 의약품 또는 화장료에 사용할 수 있다.
According to the present invention, there is provided an antioxidant, a moisturizing and / or wrinkle-improving effect of the fermented green tea extract, a fermented green tea extract or a mixture thereof, Can be used.
본 발명은 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물을 유효성분으로 포함하는 화장료 조성물 또는 피부외용제에 관한 것이다.The present invention relates to a cosmetic composition or an external preparation for skin comprising as an active ingredient an extract of Siberian ginseng, a fermented Siberian ginseng extract or a mixture thereof.
상기 홍설차 추출물 또는 발효된 홍설차 추출물은 홍설차 또는 발효된 홍설차를 용매에 추출한 것으로, 이때 사용할 수 있는 용매로는 물; 메탄올, 에탄올, 프로필알코올, 부틸알코올 등의 탄소수 1 내지 4 의 저급 알코올; 글리세린, 부틸렌글리콜, 프로필렌글리콜 등의 다가 알코올; 및 메틸아세테이트, 에틸아세테이트, 아세톤, 벤젠, 헥산, 디에틸에테르, 디클로로메탄 등의 탄화수소계 용매를 단독으로 사용하거나 2 종 이상 혼합하여 사용할 수 있다.The extracts of the present invention or the fermented extracts of the present invention are obtained by extracting the fermented red liquor or fermented red liquor into a solvent. Lower alcohols having 1 to 4 carbon atoms such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether and dichloromethane may be used alone or in combination of two or more.
상기 발효된 홍설차 추출물은 홍설차를 버섯, 유산균, 젖산균 및 효모로 이루어진 군에서 선택된 1종 이상으로 발효하여 얻은 추출물일 수 있다. 상기 버섯은 송이버섯, 치마버섯, 차가버섯, 잎새버섯 또는 이의 혼합된 균주인 것이 바람직하다. 상기 송이버섯(Tricholoma matsutake)은 담자균류(Basidiomycetes) 송이과에 속한 버섯이고, 치마버섯(Schizophyllum commune)은 담자균류(Basidiomycetes) 치마버섯과의 버섯이고, 차가버섯(Inonotus Obliquus)은 담자균류(Basidiomycetes) 소나무비늘버섯과이고, 잎새버섯(Grifola frondosa)은 담자균류(Basidiomycetes) 구멍장이버섯과의 버섯이다.The fermented Hongdong tea extract may be an extract obtained by fermenting Hongdong tea with at least one selected from the group consisting of mushroom, lactic acid bacteria, lactic acid bacteria, and yeast. Preferably, the mushroom is matsutake mushroom, skirt mushroom, chaga mushroom, mushroom mushroom or a mixed strain thereof. Tricholoma matsutake is a mushroom belonging to Basidiomycetes and Schizophyllum commune is Basidiomycetes mushroom mushroom and Inonotus Obliquus is basidiomycetes. Grifola frondosa is a mushroom of Basidiomycetes orifice and mushroom.
상기 발효된 홍설차는 송이버섯 균사, 치마버섯 균사, 차가버섯 균사, 잎새버섯 균사 또는 이들이 혼합된 균사가 접종된 홍설차가 배양된 후, 바람직하게 홍설차의 내부까지 버섯이 발효된 것을 의미한다. 상기 홍설차로서 통홍설차, 세절된 홍설차 또는 분말화된 홍설차 등을 사용할 수 있으며, 본 발명의 내용이 상기 예시된 홍설차의 형태에 제한되는 것은 아니다. 상기 발효는 10 ℃ 내지 35 ℃에서 5일 내지 40일 또는 5일 내지 30일 동안 이루어지는 것이 바람직하며, 상기 발효에 걸리는 시간은 발효 온도에 따라 달라질 수 있다. 또한, 본 발명에 사용되는 홍설차와 버섯 균사의 상태에 따라 상기 발효 조건은 달라질 수 있으므로, 본 발명의 내용이 상기 예시된 발효 온도 및 발효 시간에 제한되는 것은 아니다. The fermented mushroom is preferably fermented to the inside of the pycnometer after the cultivated mushroom mycelia, mycelia of mycelia, mycelium of mushroom, mycelia of mycelia of leaves or hyphae mixed with mycelia. As the above, there may be used, for example, a tong hong tong, a sphygmomanometer or a powdered sphygmomanometer, and the contents of the present invention are not limited to the above-described examples. The fermentation is preferably performed at 10 ° C to 35 ° C for 5 days to 40 days or 5 days to 30 days. The fermentation time may vary depending on the fermentation temperature. In addition, the fermentation conditions may vary depending on the conditions of the fermentation broth and the mushroom mycelia used in the present invention, so that the content of the present invention is not limited to the fermentation temperature and fermentation time exemplified above.
본 발명은 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물을 유효성분으로 포함하는 주름 개선, 항산화, 보습용 화장료의 조성물을 제공할 수 있다.The present invention can provide a composition of a cosmetic composition for improving wrinkles, antioxidation, and moisturizing, which comprises an extract of Hongyosuyong tea, fermented Hongdong tea extract or a mixture thereof as an active ingredient.
본 발명에 있어서, '주름 개선 효과'라 함은 피부에 주름이 생성되는 것을 억제 또는 저해하거나, 이미 생성된 주름을 완화시키는 것을 말한다. In the present invention, the term " wrinkle-reducing effect " refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating already-generated wrinkles.
본 발명에 있어서, '항산화 효과'라 함은 세포내 대사 또는 자외선의 영향으로 인한 산화적 스트레스에 따라 반응성이 높은 자유 라디칼(free radical) 또는 활성산소종(reactive oxygen species;ROS)에 의한 세포의 산화를 억제하는 것을 말하며, 자유 라디칼 또는 활성산소종을 제거하여 이로 인한 세포의 손상이 감소되는 것을 포함한다.In the present invention, the term 'antioxidative effect' refers to the action of free radicals or reactive oxygen species (ROS), which are highly reactive according to oxidative stress caused by intracellular metabolism or ultraviolet rays, Refers to inhibition of oxidation, and includes removal of free radical or reactive oxygen species, thereby reducing damage to the cells.
본 발명의 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물의 유효성분은 화장료 조성물 총 중량 대비 0.0001 중량% 내지 30 중량%로 포함되며, 화장료 조성물 총 중량 대비 0.005 중량% 내지 10 중량%로 포함되는 것이 보다 바람직하며, 0.01 중량% 내지 5 중량%로 포함되는 것이 더욱 바람직하다. 그 함량이 0.0001 중량% 미만일 경우에는 실질적인 피부 항산화 효과와 피부 보습 효과를 기대하기 어려우며, 30 중량%를 초과할 경우에는 화장학적 효과 대비 제조 비용이 너무 상승하는 문제점이 발생할 수 있다.The effective ingredient of the present invention is 0.0001 to 30% by weight based on the total weight of the cosmetic composition, and is contained in an amount of 0.005 to 10% by weight based on the total weight of the cosmetic composition. , And more preferably 0.01 wt% to 5 wt%. When the content is less than 0.0001% by weight, substantial skin antioxidative effect and skin moisturizing effect are difficult to expect. If the content is more than 30% by weight, the manufacturing cost may be too high compared to the cosmetic effect.
홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물을 화장품으로 사용하는 경우, 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물을 유효성분으로 함유하여 제조되는 화장품은 일반적인 유화 제형 및 가용화 제형의 형태로 제조할 수 있다. 예컨대, 유연 화장수 또는 영양 화장수 등과 같은 화장수, 훼이셜 로션, 바디로션 등과 같은 유액, 영양 크림, 수분 크림, 아이 크림, 마사지 크림 등과 같은 크림, 에센스, 세럼, 화장연고, 스프레이, 오일젤, 젤, 팩, 선 스크린, 메이크업 베이스, 액체 타입, 고체 타입 또는 스프레이 타입 등의 파운데이션, 파우더, 클렌징 크림, 클렌징 로션, 클렌징 오일과 같은 메이크업 제거제, 클렌징 폼, 비누, 바디 워쉬 등과 같은 세정제 등의 제형을 가질 수 있다. When the extract is used as a cosmetic product, the cosmetic product prepared by containing the extract of the present invention, the fermented product of the present invention or a mixture thereof as an active ingredient may be in the form of a general emulsified formulation and a solubilized formulation Can be manufactured. For example, creams, essences, serums, cosmetic creams, sprays, oil gels, gels, packs such as lotions such as lotion, facial lotion, body lotion and the like such as flexible lotion or nutrition lotion, nutrition cream, Such as powders, cleansing lotions, make-up removers such as cleansing oils, cleansing foams, soaps, bodywashes, and the like, such as sunscreen, makeup base, liquid type, solid type or spray type have.
상기 유연화장수는 본 발명의 홍설차 추출물 또는 발효된 홍설차 추출물 또는 이의 혼합물 이외에 프로필렌글리콜, 글리세린 등의 다가 알코올 1 ~ 10 중량% 및 폴리에틸렌올레일에테르, 폴리옥시에틸렌 경화피마자유 등의 계면활성제 0.05 ~ 2 중량%를 함유하도록 제조할 수 있다.The softening agent may be selected from the group consisting of 1 to 10% by weight of a polyhydric alcohol such as propylene glycol, glycerin and the like and a surfactant 0.05 such as polyethylene oleyl ether or polyoxyethylene hardened castor oil, To 2% by weight.
상기 영양화장수 또는 영양크림은 본 발명의 홍설차 추출물 또는 발효된 홍설차 추출물 또는 이의 혼합물 이외에 스쿠알란, 바셀린, 옥틸도데칸올 등의 오일 5 ~ 20 중량% 및 세탄올, 스테아릴알코올, 밀납 등의 왁스 3 ~ 15 중량%를 함유하도록 제조할 수 있다.The nutritional lotion or nutritional cream may contain 5 to 20% by weight of an oil such as squalane, petroleum jelly, octyldodecanol, or a mixture of cetanol, stearyl alcohol, beeswax, etc., And 3 to 15% by weight of wax.
상기 마사지크림은 본 발명의 홍설차 추출물 또는 발효된 홍설차 추출물 또는 이의 혼합물 이외에 유동파라핀, 바셀린, 이소노닐이소노나노에이트 등의 오일 30 ~ 70 중량%를 함유하도록 제조할 수 있다. The massage cream may be prepared so as to contain 30 to 70% by weight of an oil such as liquid paraffin, petrolatum, isononylisononanoate and the like in addition to the extract of the present invention or the fermented Hongdong tea extract or a mixture thereof.
상기 에센스는 본 발명의 홍설차 추출물 또는 발효된 홍설차 추출물 또는 이의 혼합물 이외에 프로필렌글리콜, 글리세린 등의 다가 알코올 3 ~ 30 중량%를 함유하도록 제조할 수 있다. The essence may be prepared so as to contain 3 to 30% by weight of a polyhydric alcohol such as propylene glycol or glycerin in addition to the extract of the present invention or the fermented extract of the present invention or a mixture thereof.
상기 팩은 본 발명의 홍설차 추출물 또는 발효된 홍설차 추출물 또는 이의 혼합물 이외에 폴리비닐알코올 5 ~ 20 중량%를 함유하는 필 오프(peel off) 팩 또는 일반유화형 화장료에 카올린, 탈크, 산화아연, 이산화티탄 등의 안료가 5 ~ 30 중량% 함유된 워시 오프(wash off) 팩으로 제조할 수 있다. The pack may be a peel off pack containing 5 to 20% by weight of polyvinyl alcohol or a common emulsifying cosmetic composition containing kaolin, talc, zinc oxide, And a wash off pack containing 5 to 30% by weight of a pigment such as titanium dioxide.
또한 본 발명의 화장료 조성물은 각각의 제형에 상기 기재한 성분들 이외에 일반적으로 화장료 조성물에 배합되는 유분, 물, 계면활성제, 보습제, 탄소수 1 내지 4의 저급 알코올, 증점제, 킬레이트제, 색소, 방부제 또는 향료 등을 필요에 따라 적절히 배합하여 사용할 수 있다.
In addition, the cosmetic composition of the present invention may further contain, in addition to the components described above, the components described above, such as oil, water, a surfactant, a moisturizer, a lower alcohol having 1 to 4 carbon atoms, a thickener, a chelating agent, Perfume and the like can be appropriately mixed and used as needed.
본 발명은 또한 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물을 유효성분으로 포함하는 주름 개선, 항산화, 보습용 피부 외용제를 제공할 수 있다.The present invention can also provide a skin external preparation for wrinkle improvement, antioxidation, and moisturizing, which comprises an extract of Hongyuyong tea, a fermented Hongdong tea extract or a mixture thereof as an active ingredient.
홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물을 피부외용제로 사용하는 경우, 추가로 지방 물질, 유기 용매, 용해제, 농축제 및 겔화제, 연화제, 항산화제, 현탁화제, 안정화제, 발포제(foaming agent), 방향제, 계면활성제, 물, 이온형 또는 비이온형 유화제, 충전제, 금속이온봉쇄제 및 킬레이트화제, 보존제, 비타민, 차단제, 습윤화제, 필수 오일, 염료, 안료, 친수성 또는 친유성 활성제, 지질 소낭 또는 피부용 외용제에 통상적으로 사용되는 임의의 다른 성분과 같은 피부 과학 분야에서 통상적으로 사용되는 보조제를 함유할 수 있다. 또한 상기 성분들은 피부 과학 분야에서 일반적으로 사용되는 양으로 도입될 수 있다. When the extract is used as an external preparation for skin, it may further contain a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent a surfactant, a water, an ionic or nonionic emulsifier, a filler, a sequestering and chelating agent, a preservative, a vitamin, a barrier, a wetting agent, an essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent, Such as lipid vesicles or any other ingredient conventionally used in external preparations for skin. The components can also be introduced in amounts commonly used in the field of dermatology.
홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물이 피부 외용제 제형으로 제공될 경우, 이에 제한되는 것은 아니나, 연고, 패취제, 겔, 크림 또는 분무제와 같은 제형을 가질 수 있다.When the extract is used as an external preparation for skin, it may have a formulation such as, but not limited to, an ointment, a patch, a gel, a cream or a spray.
본 발명의 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물의 유효성분은 피부외용제로 사용시 조성물 총 중량 대비 0.0001 내지 30 중량% 로 포함되며, 총 중량 대비 0.005 내지 10 중량%로 포함되는 것이 보다 바람직하며, 0.01 내지 5 중량%로 포함되는 것이 더욱 바람직하다. 그 함량이 0.0001 중량% 미만일 경우에는 실질적인 피부 항산화 효과와 피부 보습 효과를 기대하기 어려우며, 30 중량%를 초과할 경우에는 의약적 효과 대비 제조 비용이 너무 상승하는 문제점이 발생할 수 있다.The active ingredient of the extract of the present invention, fermented red ginseng extract or a mixture thereof is used as an external preparation for skin, when used in an amount of 0.0001 to 30% by weight based on the total weight of the composition, more preferably 0.005 to 10% by weight By weight, more preferably 0.01 to 5% by weight. When the content is less than 0.0001% by weight, substantial skin antioxidative effect and skin moisturizing effect are difficult to be expected. If the content is more than 30% by weight, the manufacturing cost may be too high compared with the medicinal effect.
본 발명의 피부외용제는 유효량의 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물을 포함할 때 바람직한 주름 개선 효과, 항산화 효과, 보습 효과를 제공할 수 있다. 본 발명에 있어서, '유효량'이라 함은 주름을 개선하거나, 세포의 산화를 억제 또는 완화하거나, 보습 효과를 나타낼 수 있는 화합물의 양을 의미한다. 본 발명의 조성물에 포함되는 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물의 유효량은 조성물이 제품화되는 형태, 상기 화합물이 피부에 적용되는 방법 및 피부에 머무르는 시간 등에 따라 달라질 것이다. 예컨대, 상기 조성물이 의약품으로 제품화되는 경우에는 일상적으로 피부에 적용하게 되는 화장품으로 제품화되는 경우에 비해 높은 농도로 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물을 포함할 수 있을 것이다. 따라서, 일일 투여량은 홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물의 양을 기준으로 0.1 내지 100 ㎎/㎏이고, 바람직하게는 30 내지 80 ㎎/㎏이고, 더욱 바람직하게는 50 내지 60 mg/kg이며, 하루 1~6 회 투여될 수 있다.
The external preparation for skin of the present invention can provide a desirable wrinkle-improving effect, antioxidative effect, and moisturizing effect when the effective amount of the present invention is contained in an effective amount of the extract. In the present invention, the term "effective amount" means the amount of a compound capable of improving wrinkles, inhibiting or alleviating the oxidation of cells, or exhibiting a moisturizing effect. The effective amount of the composition of the present invention, the fermented red ginseng extract, or a mixture thereof, will vary depending on the form in which the composition is produced, how the compound is applied to the skin, and the time on the skin. For example, when the composition is commercialized as a pharmaceutical product, it may contain a high concentration of the extract of the present invention, the extract of the present invention, or a mixture thereof, as compared with the case where the composition is made into a cosmetic product that is routinely applied to the skin. Accordingly, the daily dosage is 0.1 to 100 mg / kg, preferably 30 to 80 mg / kg, more preferably 50 to 60 mg / kg, based on the amount of the extract, fermented red ginseng extract or mixture thereof. / kg, and can be administered 1 to 6 times a day.
이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.
Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.
참조예Reference Example : 재료 구입: Purchasing materials
홍설차와 버섯 균주의 경우 원료업체인 주식회사 래디안을 통해 구입하였다. 래디안에서는 홍설차를 중국에서 직접 구매하였고, 버섯 균주의 경우 4가지 균사체는 모두 생원물 버섯 상태로 구입 혹은 분양받아 각 버섯에서 직접 균사체 분리하여 배양한 후 발효물 제작을 위한 균사체로 사용하였다
In the case of red mulberry and mushroom strains, it was purchased through Radian, a raw material company. In the case of mushroom strain, all four mycelium were purchased or sold in the form of fresh mushroom, and mycelium was directly isolated from each mushroom and used as a mycelium for fermentation
실시예Example 1: One: 홍설차Hong Sung Cha 추출물의 제조 Preparation of extract
홍설차[(주)래디안에서 구입] 200 g을 세말하여 분말화한 후, 여기에 증류수와 에탄올이 3:7 부피비로 혼합된 추출용매를 넣어 실온(20 ~ 25℃)에서 5시간 동안 추출하고 1차 여과를 통해 추출된 70% 에탄올 추출물을 증발과정을 통해 에탄올을 제거한 뒤 다시 원심분리와 2차 여과를 통해 침전물을 제거하였다. 마지막으로 마이크로 여과를 거쳐 홍설차 추출물 23 g을 수득하였다.
(200 g) was powdered and extracted with an extraction solvent mixed with distilled water and ethanol at a ratio of 3: 7 by volume, and the mixture was extracted at room temperature (20 to 25 ° C) for 5 hours The ethanol extract was removed from the 70% ethanol extract through the primary filtration, and then the precipitate was removed by centrifugation and secondary filtration. Finally, 23 g of the extract was obtained by microfiltration.
실시예Example 2: 송이버섯으로 발효된 2: fermented with matsutake mushroom 홍설차Hong Sung Cha 추출물의 제조 Preparation of extract
송이버섯 균주로 트리코로마 마추타케(Tricholoma matsutake) [(주)래디안에서 구입]를 PDA(potato dextrose agar) 고체 배지에서 25℃의 온도 조건으로 배양하였다. 배양된 고체 균을 PDB(potato dextrose broth) 액체 배지에 접종하고 포자 농도가 10 CFU/mL가 될 때까지 25℃, 130 rpm으로 진탕 배양하였다. 그 다음, 상기 배양한 종균을 1 mL의 1회용 주사기로 홍설차[(주)래디안에서 구입]에 150㎕ 주사한 후 24℃, 85 ~ 90% 습도에서 일주일간 보호하고, 자실체가 완성될 때까지 20℃, 85 ~ 90% 습도에서 보관하였다. 송이버섯의 균사가 홍설차의 내부까지 침투하도록 하여 홍설차의 내부까지 전체적으로 발효되도록 25 ~ 35℃의 온도를 유지하면서 5일 동안 발효를 진행하여 송이버섯으로 발효된 홍설차를 제조하였다. Tricholoma matsutake (purchased from Rady) was cultivated in a PDA (potato dextrose agar) solid medium at a temperature of 25 ° C. The cultured solid bacteria were inoculated into a PDB (potato dextrose broth) liquid medium and cultured at 25 ° C with shrimp at 130 rpm until the spore concentration reached 10 CFU / mL. Then, the cultured seedlings were injected with 1 mL of a single-use syringe at 150 rpm in Hongryu tea [purchased in Radia Co., Ltd.] and then protected for one week at 24 ° C and 85 to 90% humidity for one week. When the fruiting body was completed At 20 ° C and 85-90% humidity. The mycelium of pine mushroom was infiltrated to the inner part of the pine tree and fermented for 5 days while maintaining the temperature at 25 ~ 35 ℃ for the entire fermentation to the inner part of pine tree.
상기 제조된 송이버섯 발효된 홍설차를 건조한 후 200 g을 세말하여 분말화한 후, 여기에 증류수와 에탄올이 3:7 부피비로 혼합된 추출용매를 넣어 실온(20 ~ 25℃)에서 5시간 동안 추출하고 1차 여과를 통해 추출된 70% 에탄올 추출물을 증발과정을 통해 에탄올을 제거한 뒤 다시 원심분리와 2차 여과를 통해 침전물을 제거하였다. 마지막으로 마이크로 여과를 거쳐 송이버섯으로 발효된 홍설차 추출물 35 g을 수득하였다.
The dried pine mushroom fermented Hong Suk Cha was dried, and then 200 g of the extract was powdered. The extraction solvent mixed with 3: 7 volume ratio of distilled water and ethanol was added thereto, and the mixture was stirred at room temperature (20 to 25 ° C) for 5 hours The 70% ethanol extracts extracted by primary filtration were removed by evaporation and then the precipitates were removed by centrifugation and secondary filtration. Finally, micro-filtration was conducted to obtain 35 g of an extract of Pseudomonas fluorescens fermented with pine mushroom.
실시예Example 3: 3: 치마버섯으로With a skirt mushroom 발효된 Fermented 홍설차Hong Sung Cha 추출물의 제조 Preparation of extract
상기 실시예 2과 동일한 방법으로 실시하되, 송이버섯 대신 치마버섯을 사용(치마버섯 균주로 Schizophyllum commune[(주)래디안에서 구입] 사용)하여 치마버섯으로 발효된 홍설차 추출물 37 g을 수득하였다.
Except that Schizophyllum commune (purchased from Rady) was used as a skimmed mushroom strain instead of pine mushroom to obtain 37 g of an extract of Siberian myrtle fermented with skimmed mushroom.
실시예Example 4: 4: 차가버섯으로With mushroom 발효된 Fermented 홍설차Hong Sung Cha 추출물의 제조 Preparation of extract
상기 실시예 2과 동일한 방법으로 실시하되, 송이버섯 대신 차가버섯을 사용(차가버섯 균주로 Inonotus Obliquus[(주)래디안에서 구입] 사용)하여 차가버섯으로 발효된 홍설차 추출물 35 g을 수득하였다.
The same procedure as in Example 2 was carried out except that 35 g of an extract of Lycoris sieboldii fermented with mash mushroom was obtained using chrysanthemum mushroom (purchased from Inonotus Obliquus (purchased from Rady) as a mushroom strain instead of mushroom.
실시예Example 5: 5: 잎새버섯으로With mushrooms 발효된 Fermented 홍설차Hong Sung Cha 추출물의 제조 Preparation of extract
상기 실시예 2과 동일한 방법으로 실시하되, 송이버섯 대신 잎새버섯을 사용(잎새버섯 균주로 Grifola frondosa[(주)래디안에서 구입] 사용)하여 잎새버섯으로 발효된 홍설차 추출물 38 g을 수득하였다.
The same procedure as in Example 2 was carried out except that 38 g of an extract of Lycoris sieboldii fermented with a mushroom was obtained by using a leaf mushroom instead of a mushroom (using Grifola frondosa [purchased from Radia Inc.] as a leaf mushroom strain).
실시예Example 6: 혼합 6: Mixed 버섯균주로With mushroom strain 발효된 Fermented 홍설차Hong Sung Cha 추출물의 제조 Preparation of extract
상기 실시예 2과 동일한 방법으로 실시하되, 송이버섯 대신 송이버섯, 치마버섯, 차가버섯 및 잎새버섯의 균주가 각각 1:1:1:1의 질량비로 혼합된 혼합 버섯균주를 사용하여 혼합 균주로 발효된 홍설차 추출물 36 g을 수득하였다.
The same procedure as in Example 2 was carried out except that, in place of the matsutake mushroom, the strain matsutake mushroom, the mushroom mushroom, the mushroom mushroom and the mushroom strain were mixed at a mass ratio of 1: 1: 1: 36 g of a fermented green tea extract was obtained.
실험예Experimental Example 1: 세포 독성 시험 1: Cytotoxicity test
상기 실시예 1의 홍설차 추출물, 실시예 2의 송이버섯으로 발효된 홍설차 추출물, 실시예 3의 치마버섯으로 발효된 홍설차 추출물, 실시예 4의 차가버섯으로 발효된 홍설차 추출물, 실시예 5의 잎새버섯으로 발효된 홍설차 추출물, 실시예 6의 버섯 혼합 균주로 발효된 홍설차 추출물의 안전성을 검증하기 위하여, 각각의 추출물에 대하여 V79-4세포(차이니스 햄스터, 폐 조직 섬유아세포의 연속 세포주)를 배양하고 MTT 시험법을 수행하여 세포 독성을 조사하고, 그 결과를 하기 표 1에 나타냈다. 소디움라우릴설페이트(SLS)에 대해서도 같은 방법으로 세포 독성 시험을 실시하였다.The extracts of Example 1, Example 2, Example 1, Example 1, Example 1, Example 2, Example 3, Example 3, Example 3, Example 4, In order to examine the safety of the extracts of fermented Lycoris sieboldii fermented with 5 mushroom and fermented with the mushroom-mixed strain of Example 6, the extracts of V79-4 cells (Chinese hamster, lung fibroblasts Continuous cell line) was cultured and MTT assay was performed to examine cytotoxicity, and the results are shown in Table 1 below. Sodium lauryl sulfate (SLS) was also tested for cytotoxicity in the same manner.
상기 표 1로부터, 피부외용제 또는 화장료 조성물에 사용되는 추출물(실시예 1 ~ 6)은 계면활성제인 소디움라우릴설페이트(SLS)에 비하여 약 200~300배 낮은 세포 독성을 보여 안전성이 우수함을 확인할 수 있었다.
It can be seen from Table 1 that the extracts used in external preparations for skin or cosmetic compositions (Examples 1 to 6) show about 200 to 300 times lower cytotoxicity than sodium lauryl sulfate (SLS), which is a surfactant, there was.
실험예Experimental Example 2: 2: 알러지allergy 평가(H- Evaluation (H- CLATCLAT ))
상기 실험예 1의 세포 독성 시험에서 안전한 것으로 확인된 본 발명의 홍설차 추출물(실시예 1 ~ 6)의 알러지 유발 여부를 확인하기 위한 실험으로서, H-CLAT(Human Cell Line Activation Test) 실험법을 실시하였다. As an experiment for confirming whether or not all of the extracts of the present invention (Examples 1 to 6), which were confirmed to be safe in the cytotoxicity test of the above Experimental Example 1, were caused allergy, H-CLAT (Human Cell Line Activation Test) Respectively.
먼저, 평가 대상 물질의 처리할 농도를 설정하기 위하여 사전 세포독성 실험을 실시하여 CV75(세포의 생존률이 75%인 농도)를 기준으로, CV75*1.2, CV75, CV75/1.23, CV75/1.25 및 수용성의 경우 10,000㎍/ml, 비수용성의 경우 1,000㎍/ml인 HTD(Highest technical dose)의 농도를 기준으로 위 농도까지 독성이 나타나지 않으면 이를 기준으로 처리농도를 설정하였다. 사전 세포독성 실험에서 설정된 처리농도로 THP-1(human monocytic cell line)에 물질을 접촉시키고 세포를 24시간 배양한 뒤 물질과 접촉한 THP-1 세포를 형광물질(FITC)가 표지된 항체로 염색하여, Isotype 대조군, CD54, CD86 검출을 Flow cytomertic 분석으로 확인하고 CD54, CD86의 검출량을 등급화하여 분류하였다. 이때, 알레르기 유발성 판정 기준에 따라 CD54 RFI(Relative fluorescence intensity) ≥ 200 및/또는 CD86 RFI(Relative fluorescence intensity) ≥ 150 이면 양성으로 판정하였다. First, by implementing the pre-cytotoxicity test in order to set the concentration to process the evaluation target substance based on the CV75 (survival rate is 75% of the concentration of cells), CV75 * 1.2, CV75, CV75 / 1.2 3, CV75 / 1.2 5 And the concentration of HTD (Highest technical dose), which is 10,000 μg / ml in the case of water solubility and 1,000 μg / ml in the case of non-water solubility, was set as the reference concentration. The cells were contacted with THP-1 (human monocytic cell line) at a treatment concentration set in the pre-cytotoxicity experiment, and the cells were cultured for 24 hours. Then, the THP-1 cells in contact with the material were stained with a fluorescent antibody (FITC) Isotype control, CD54, and CD86 detection were confirmed by flow cytomatic analysis, and the detection quantities of CD54 and CD86 were classified and classified. At this time, the CD54 RFI (Relative fluorescence intensity) ≥ 200 and / or CD86 RFI (Relative fluorescence intensity) ≥ 150 were judged to be positive according to the criteria for allergy induction.
알러지 평가 결과, 세포생존도 80% 이상에서 모든 농도에서 CD54 RFI ≤ 200, CD86 RFI ≤ 150 이므로 알러지 유발가능성이 없는 것으로 나타났다.
As a result of allergic evaluation, CD54 RFI ≤ 200 and CD86 RFI ≤ 150 were found to be no possibility of allergy at all concentrations at cell concentration more than 80%.
실험예Experimental Example 3: 3: 자유라디칼Free radical 소거능Scatters 확인 Confirm
상기 실시예 1 ~ 6의 홍설차 추출물에 대한 자유라디칼 소거능을 1,1-디페닐-2-피크릴히드라질(DPPH) 방법으로 측정하였다(Blois, Nature 181, 1190, 1958). DPPH는 비교적 안정한 자유라디칼로서 라디칼 상태로 존재 시 517㎚에서 최대 흡광을 보이며 라디칼이 소거되면 흡광성을 잃는다. DPPH는 시그마(Sigma)사의 것을 사용하였으며, 0.15 mM의 농도로 메틸 알코올에 녹여 사용하였다.The free radical scavenging activity of the extracts of Examples 1 to 6 was measured by 1,1-diphenyl-2-picryl hydrazine (DPIH) method (Blois, Nature 181, 1190, 1958). DPPH is a relatively stable free radical, which shows maximum absorption at 517 nm in the presence of radicals and loses its absorbance when radicals are eliminated. DPPH was purchased from Sigma, and dissolved in methyl alcohol at a concentration of 0.15 mM.
먼저, 상기 추출물 및 비타민 C를 96-웰 플레이트의 각각의 웰에 100㎕씩 넣었다. 여기에 DPPH 용액을 100㎕씩 첨가한 다음 상온에서 30분간 방치한 후 Micro plate reader(BioTek EL-340)를 이용하여 517㎚에서의 흡광도를 측정하였다. 이때, 대조군으로는 메틸 알코올을 사용하였다. First, 100 μl of the extract and vitamin C were added to each well of a 96-well plate. 100 μl of DPPH solution was added thereto, and the mixture was allowed to stand at room temperature for 30 minutes. Then, the absorbance at 517 nm was measured using a Microplate reader (BioTek EL-340). At this time, methyl alcohol was used as a control group.
시료를 처리한 것의 흡광도가 대조군의 흡광도의 절반이 될 때의 추출물의 농도를 IC50(Inhibitory Concentration 50)으로 표시하였으며, 그 결과를 하기 표 3에 나타냈다. The concentration of the extract when the absorbance of the sample was half the absorbance of the control group was expressed by IC 50 (Inhibitory Concentration 50), and the results are shown in Table 3 below.
상기 표 3에 나타낸 바와 같이, 본 발명의 홍설차 추출물은, 자유라디칼 제거제로 알려진 비타민 C와 유사한 정도로 자유라디칼 소거능을 나타내어, 자유라디칼 소거능이 매우 강력하였음을 알 수 있었다.
As shown in Table 3, the extract of the present invention showed free radical scavenging ability similar to that of vitamin C, which is known as a free radical scavenger, so that the free radical scavenging ability was very strong.
실험예Experimental Example 4: 피부 자극성 평가 4: Evaluation of skin irritation
본 발명의 홍설차 추출물예 대하여 피부 자극성을 알아보기 위해 건강한 성인 남, 여 50명을 대상으로 등 부위에 각 시료의 일정량(0.2g)을 24시간 첩포한 후, 핀 챔버를 제거하고 4시간 경과한 다음 육안으로 피부 상태 변화를 판독하여, 그 결과를 하기 표 4에 나타내었다.In order to examine the skin irritation of the present invention, a predetermined amount (0.2 g) of each sample was applied to the back part of a healthy adult male and female for 24 hours, and then the pin chamber was removed. Then, changes in skin condition were visually observed, and the results are shown in Table 4 below.
(증류수)Negative control group
(Distilled water)
(SLS 0.1%)Positive control group
(SLS 0.1%)
- : 홍반이나 특이한 현상 없음
+- : 주위보다 약간 붉어짐
+ : 주위보다 현저히 붉어짐
++ : 주위보다 심하게 붉어지고 부풀어 오름[week]
-: No erythema or unusual phenomenon
+ -: slightly reddish from the surroundings
+: Significantly reddened than surrounding
++: More reddening and swelling than the surrounding area
상기 표 4에 나타낸 바와 같이, 본 발명의 실시예 1 ~ 6이 음성대조군인 증류수와 비슷한 정도의 낮은 피부 자극도를 나타낸 것으로 보아 피부에 안전하다는 것을 확인할 수 있었다.
As shown in Table 4, Examples 1 to 6 of the present invention showed low skin irritancy similar to that of distilled water as a negative control group.
실시예Example 7 ~ 13 및 7-13 and 비교예Comparative Example 1: 수중유형 1: Underwater type 유화물Emulsion 제조 Produce
상기 실시예 1 ~ 6의 추출물, 및 실시예 1과 실시예 6의 추출물을 1:1의 중량 비율로 혼합한 혼합물을 각각을 포함하는 수중유형 유화물(실시예 7 ~ 13)을 제조하였다. 또한, 상기 추출물을 포함하지 않는 수중유형 유화물을 비교예 1로 제조하였다. 상세한 조성(중량%)은 하기 표 5에 나타냈다.(Examples 7 to 13) each containing a mixture of the extracts of Examples 1 to 6 and the extracts of Example 1 and Example 6 in a weight ratio of 1: 1 were prepared. In addition, the water-in-oil emulsion containing no such extract was prepared as Comparative Example 1. The detailed composition (% by weight) is shown in Table 5 below.
(중량%)division
(weight%)
7Example
7
8Example
8
9Example
9
10Example
10
11Example
11
12Example
12
13Example
13
1Comparative Example
One
1. 카르복시비닐폴리머 - 미국 비. 에프. 굿리치 (B.F.Goodrich) 사의 카보폴 941 (Carbopol 941) 1 중량 % 수용액
2. 모노스테아린산소르비탄 - 영국 아이시아이 (ICI) 사이 아라셀 60(Arlacel 60)
3. 이소세틸옥타노에이트 - 일본 니혼 고쿄 알코올 (Nihon KoKyu Alcohol) 사의 아이시이에이치 (ICEH)[week]
1. Carboxyvinyl polymer - US Ratio. F. A 1% by weight aqueous solution of Carbopol 941 from BF Goodrich Co.
2. Sorbitan monostearate - ICI Sialasel 60 (Arlacel 60)
3. Isocetyl octanoate - Ishihichi (ICEH) from Nihon KoKyu Alcohol Co.,
실험예Experimental Example 5: 주름 개선 효과 5: Wrinkle improvement effect
상기 실시예 7 ~ 13 및 비교예 1의 수중유형 유화물을 건강한 35 ~ 50 세의 여성을 대상으로 하여 다음과 같은 방법으로 주름 개선 효과를 측정하였다.The water-in-oil type emulsions of Examples 7 to 13 and Comparative Example 1 were tested for the effect of improving wrinkles in healthy 35 to 50 year old women by the following method.
35 ~ 50 세의 여성 80 명을 10 명씩 8 개의 군으로 나눈 후, 7 개의 군은 실시예 7 ~ 13의 수중유형 유화물을 안면부에 1일 1 회씩 3 개월간 도포하도록 하였고, 나머지 1개의 군은 비교예 1의 수중유형 유화물을 안면부에 1일 1 회씩 3 개월간 도포하도록 하였다. 80 women aged 35 to 50 years were divided into 8 groups by 10 persons. Seven groups were applied with the emulsions of Examples 7 to 13 for 3 months once a day in the face area, and the remaining groups were compared The water-in-oil emulsion of Example 1 was applied to the facial region once a day for three months.
3 개월 후 주름의 개선 정도를 피시험자의 설문 및 주름의 영상분석을 통해 평가하였다. 피시험자의 설문은 주름 개선 및 탄력 증진에 관하여 사용 전과 비교하여 개선 없음, 약간의 개선, 상당한 개선의 3 단계로 판정하였으며, 그 결과를 하기 표 6에 나타내었다. After 3 months, the improvement of wrinkles was evaluated by image analysis of questionnaires and wrinkles. The questionnaire of the subject was evaluated in three stages of improvement of wrinkles and improvement of elasticity, no improvement, slight improvement and considerable improvement as compared with before use, and the results are shown in Table 6 below.
상기 표 6에 나타낸 바와 같이, 본 발명의 실시예 7 ~ 13의 수중유형 유화물은 비교예 1의 수중유형 유화물에 비해 주름 개선 효과가 우수함을 확인할 수 있었다.
As shown in Table 6, it was confirmed that the underwater type emulsions of Examples 7 to 13 of the present invention had an excellent wrinkle reducing effect as compared with the underwater type emulsion of Comparative Example 1. [
실시예Example 14 ~ 20 및 14 to 20 and 비교예Comparative Example 2: 에센스의 제조 2: Preparation of essence
상기 실시예 1 ~ 6의 추출물 및 실시예 1과 실시예 6의 추출물을 1:1의 중량비율로 혼합한 혼합물을 각각을 포함하는 에센스(실시예 14 ~ 20)를 제조하였다. 또한, 상기 추출물을 포함하지 않는 에센스를 비교예 2로서 제조하였다. 상세한 조성(중량%)은 하기 표 7에 나타냈다. Essences (Examples 14 to 20) each containing a mixture of the extracts of Examples 1 to 6 and the extracts of Example 1 and Example 6 in a weight ratio of 1: 1 were prepared. In addition, an essence not containing the above extract was prepared as Comparative Example 2. The detailed composition (% by weight) is shown in Table 7 below.
(중량%)division
(weight%)
14Example
14
15Example
15
16Example
16
17Example
17
18Example
18
19Example
19
20Example
20
2Comparative Example
2
2. 모노스테아린산소르비탄 -영국 아이시아이 (ICI) 사이 아라셀 60(Arlacel 60)
3. 이소세틸옥타노에이트 -일본 니혼 고쿄 알코올 (Nihon KoKyu Alcohol) 사의 아이시이에이치 (ICEH)[Notes] 1. Carboxyvinyl polymer - United States Ratio. F. A 1% by weight aqueous solution of Carbopol 941 from BF Goodrich Co.
2. Sorbitan monostearate - ICI Sialasel 60 (Arlacel 60)
3. Isocetyl octanoate - Ishihichi (ICEH) from Nihon KoKyu Alcohol Co.,
실험예Experimental Example 6: 수분 6: Moisture 보유능Possession 평가 evaluation
상기 실시예 14 ~ 20 및 비교예 2의 에센스의 피부 보습력을 평가하기 위하여, 상기 실시예 14 ~ 20 및 비교예 2의 에센스를 각각 피부에 정량 도포한 후 각질층의 수분 보유량을 측정하고 이를 평가하는 방법을 사용하였다. 측정 기기로는 피부수분함량 측정기(스킨 설피스 하이그로미터, skin surface hygrometer, 모델명: SKICON-200, 일본 IBS사 제품)를 이용하였다. 구체적으로는, 25℃, 상대습도 45%의 항온 항습실에서 실시예 및 비교예의 에센스를 각각 20명의 피시험자의 팔의 안쪽에 일정량(2 mg/㎠) 도포한 후 잘 문지르고, 평가 전의 수분 보유능을 기준으로 1시간과 3시간 후의 수분 증가율(%)을 측정하여 보습 효과를 평가하였다. 얻어진 결과를 하기 표 8에 나타내었다.In order to evaluate the skin moisturizing power of the essences of Examples 14 to 20 and Comparative Example 2, the essence of each of Examples 14 to 20 and Comparative Example 2 was applied to the skin to quantitatively measure the water retention amount of the stratum corneum, Method. As a measuring device, a skin moisture meter (skin surface hygrometer, model name: SKICON-200, manufactured by IBS Japan) was used. Specifically, a certain amount (2 mg / cm 2) of the essences of Examples and Comparative Examples was applied to the inside of arms of 20 subjects respectively in a constant temperature and humidity room at 25 ° C. and a relative humidity of 45%, and rubbed well. The moisture increase rate (%) after 1 hour and 3 hours was measured to evaluate the moisturizing effect. The obtained results are shown in Table 8 below.
14Example
14
15Example
15
16Example
16
17Example
17
18Example
18
19Example
19
20Example
20
2Comparative Example
2
상기 표 8에 나타낸 바와 같이, 본 발명의 실시예의 에센스는 비교예의 에센스에 비해 도포 1시간 후 및 3시간 후에 수분 보유 증가율이 훨씬 높아 보습 효과가 우수함을 알 수 있었다.
As shown in Table 8, it was found that the essence of the example of the present invention was much higher in moisturizing effect than that of the essence of the comparative example, because the rate of increase in moisture retention was much higher after one hour and three hours after application.
제제예Formulation example 1: One: 화장료의Cosmetic 제조 Produce
1. 화장수의 제조 1. Manufacture of lotion
홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물 0.1 중량%, 글리세린 5.0 중량%, 디프로필렌글리콜 3.0 중량%, 히알루론산 0.5 중량%, 폴리옥시에틸렌 경화피마자유 0.1 중량%, 폴리에틸렌 올레일에테르 0.1 중량%, 에탄올 5.0 중량%, 방부제 0.15 중량%, 색소 0.05 중량％, 향료 0.05 중량％ 및 잔량의 정제수를 혼합(전체 조성 100 중량%)하여 화장수를 제조하였다.
0.1% by weight of glycerin, 3.0% by weight of dipropylene glycol, 0.5% by weight of hyaluronic acid, 0.1% by weight of polyoxyethylene hydrogenated castor oil, 0.1% by weight of polyethylene oxide oleate (Total composition: 100% by weight), 5.0% by weight of ethanol, 0.15% by weight of preservative, 0.05% by weight of pigment, 0.05% by weight of perfume and remaining balance.
2. 에센스의 제조2. Manufacture of Essence
홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물 7.0 중량%, 세토스테아릴알코올 1.0 중량%, 자기유화형 모노스테아린산 1.0 중량%, 밀납 0.5 중량%, 스쿠알란 5.0 중량%, 이소세틸옥타노에이트 3.0 중량%, 디메틸실록산 0.3 중량%, 모노스테아린산소르비탄 0.5 중량%, 모노스테아린산폴리에틸렌글리콜 8.0 중량%, 글리세린 4.0 중량%, 프로필렌글리콜 0.2 중량%, 카르복시폴리머 0.22 중량%, 트리에탄올아민 0.25 중량%, 방부제 0.05 중량％, 색소 0.05 중량％, 향료 0.05 중량％ 및 잔량의 정제수를 혼합(전체 조성 100 중량%)하여 에센스를 제조하였다.
7.0 weight% of cetyl alcohol, 1.0 weight% of self-emulsifying monostearate, 0.5 weight% of wax, 5.0 weight% of squalane, 3.0 weight of isocetyl octanoate 0.3 wt% of dimethylsiloxane, 0.5 wt% of monostearic acid sorbitan, 8.0 wt% of monostearic acid polyethylene glycol, 4.0 wt% of glycerin, 0.2 wt% of propylene glycol, 0.22 wt% of carboxy polymer, 0.25 wt% of triethanolamine, 0.05 wt% %, 0.05% by weight of coloring matter, 0.05% by weight of fragrance, and the remaining amount of purified water (total composition: 100% by weight).
3. 로션의 제조 3. Production of Lotion
홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물 5.0 중량%, 세토스테아릴알코올 0.8 중량%, 자기유화형 모노스테아린산 1.0 중량%, 밀납 0.5 중량%, 스테아린산 0.5 중량%, 유동파라핀 7.0 중량%, 스쿠알란 5.0 중량%, 마카데미아오일 3.0 중량%, 이소세틸옥타노에이트 2.0 중량%, 디메틸실록산 0.3 중량%, 모노스테아린산소르비탄 0.5 중량%, 모노스테아린산폴리에틸렌글리콜 1.2 중량%, 글리세린 4.0 중량%, 프로필렌글리콜 4.0 중량%, 베타인 4.0 중량%, 카르복시폴리모 0.12 중량%, 트리에탄올아민 0.15 중량%, 방부제 0.25 중량%, 색소0.05 중량％, 향료 0.05 중량％ 및 잔량의 정제수를 혼합(전체 조성 100 중량%)하여 로션을 제조하였다.
5.0 wt.% Of cetyl alcohol, 0.8 wt.% Of cetostearyl alcohol, 1.0 wt.% Of self emulsifying monostearate, 0.5 wt.% Of wax, 0.5 wt.% Of stearic acid, 7.0 wt.% Of liquid paraffin, 5.0% by weight, macadamia oil 3.0% by weight, isocetyl octanoate 2.0% by weight, dimethylsiloxane 0.3% by weight, monostearic acid sorbitan 0.5% by weight, monostearic acid polyethylene glycol 1.2% by weight, glycerin 4.0% by weight, propylene glycol (100% by weight of total composition) of 4.0% by weight, betaine 4.0% by weight, carboxypolymer 0.12% by weight, triethanolamine 0.15% by weight, preservative 0.25% by weight, coloring matter 0.05% by weight, To prepare a lotion.
4. 크림의 제조 4. Manufacture of cream
홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물 7.0 중량%, 세토스테아릴알코올 3.0 중량%, 자기유화형 모노스테아린산 0.5 중량%, 친유형 모노스테아린산 1.5 중량%, 밀납 0.5 중량%, 유동파라핀 8.0 중량%, 스쿠알란 7.0 중량%, 이소세틸옥타노에이트 4.0 중량%, 정제 호호바유 4.0 중량%, 디메틸실록산 0.3 중량%, 모노스테아린산소르비탄 1.0 중량%, 모노스테아린산폴리에틸렌글리콜 1.2 중량%, 글리세린 6.0 중량%, 프로필렌글리콜 4.0 중량%, 베타인 4.0 중량%, 잔탄검 0.06 중량%, 트리에탄올아민 0.10 중량%, 방부제 0.25 중량%, 향료 0.05 중량％, 색소 0.05 중량％ 및 잔량의 정제수를 혼합(전체 조성 100 중량%)하여 크림을 제조하였다.
7.0 wt.% Of cetostearyl alcohol, 3.0 wt.% Of cetostearyl alcohol, 0.5 wt.% Of self emulsifying monostearate, 1.5 wt.% Of prochymous monostearate, 0.5 wt.% Of wax, 8.0 wt.% Of liquid paraffin 4.0% by weight of squalane, 4.0% by weight of isocetyl octanoate, 4.0% by weight of purified jojoba oil, 0.3% by weight of dimethylsiloxane, 1.0% by weight of monostearic acid sorbitan, 1.2% by weight of monostearic acid polyethylene glycol, (Whole composition: 100 wt%) was mixed with 4.0 wt% of propylene glycol, 4.0 wt% of betaine, 0.06 wt% of xanthan gum, 0.10 wt% of triethanolamine, 0.25 wt% of preservative, 0.05 wt% ) To prepare a cream.
제제예Formulation example 2: 2: 피부외용제의Topical 제조 Produce
1. 겔의 제조1. Preparation of gel
홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물 1.0 중량%, 글리세린 4.0 중량%, 프로필렌글리콜 4.0 중량%, 에탄올 10 중량%, 폴리옥시에틸렌 경화피마자유 0.1 중량%, 카르복시폴리머 0.30 중량%, 트리에탄올아민 0.30 중량%, 방부제 0.05 중량％, 색소 0.05 중량％, 향료 0.05 중량％, 잔량의 정제수를 혼합(전체 조성 100 중량%)하여 젤을 제조하였다.
1.0 wt% of glycerin, 4.0 wt% of glycerin, 4.0 wt% of propylene glycol, 10 wt% of ethanol, 0.1 wt% of polyoxyethylene hardened castor oil, 0.30 wt% of carboxy polymer, 0.30% by weight of a preservative, 0.05% by weight of a preservative, 0.05% by weight of a coloring matter, 0.05% by weight of a perfume, and a remaining amount of purified water (100% by weight of total composition).
2. 연고의 제조2. Manufacture of ointment
홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물 0.5 중량％, 베타-1,3-글루칸 10.0 중량%, 밀납 10.0 중량%, 폴리솔베이트60 5.0 중량%, 피이지 60 경화피마자유 2.0 중량%, 솔비탄세스퀴올레이트 0.5 중량%, 바셀린 5.0 중량%, 유동파라핀 10.0 중량%, 스쿠알란 5.0 중량%, 쉐어버터 3.0 중량%, 카프릴릭/카프릭트리글리세라이드 5.0 중량%, 글리세린 10.0 중량%, 프로필렌글리콜 10.2 중량%, 트리에탄올아민 0.2 중량%, 방부제 0.05 중량％, 색소 0.05 중량％, 향료 0.05 중량％, 적량의 정제수를 혼합(전체 조성 100 중량%)하여 연고를 제조하였다.
0.5 wt% of beta-1, 3-glucan, 10.0 wt% of beeswax, 5.0 wt% of polysorbate 60, 2.0 wt% of hydrogenated castor oil, A composition comprising 0.5% by weight of sorbitan sesquioleate, 5.0% by weight of vaseline, 10.0% by weight of liquid paraffin, 5.0% by weight squalane, 3.0% by weight of shea butter, 5.0% by weight of caprylic / capric triglyceride, 10.0% by weight of glycerin, Ointment was prepared by mixing 10.2% by weight of diethanolamine, 0.2% by weight of triethanolamine, 0.05% by weight of preservative, 0.05% by weight of pigment, 0.05% by weight of perfume and an appropriate amount of purified water.
3. 국소 투여용 약제(패취제)의 제조3. Preparation of topical medicines (patches)
홍설차 추출물, 발효된 홍설차 추출물 또는 이의 혼합물 0.5 중량%, 베타-1,3-글루칸 3.0 중량%, 헥실렌글리콜 20.0 중량%, 디에틸아민 0.7 중량%, 폴리아크릴산(Carbopol 934P) 1.0 중량%, 아황산나트륨 0.1 중량%, 폴리옥시에틸렌라우릴에테르(E.O=9) 1.0 중량%, 폴리히드록시에틸렌세틸스테아릴에테르(Cetomacrogol 1000) 1.0 중량%, 점성의 파라핀 오일 2.5 중량%, 카프릴산에스테르/카프르산에스테르(Cetiol LC) 2.5 중량%, 폴리에틸렌글리콜400 3.0 중량%, 잔량의 탈이온수를 혼합(전체 조성 100 중량%)하여 패취제를 제조하였다.0.5% by weight of beta-1,3-glucan, 20.0% by weight of hexylene glycol, 0.7% by weight of diethylamine, 1.0% by weight of polyacrylic acid (Carbopol 934P) , 0.1% by weight of sodium sulfite, 1.0% by weight of polyoxyethylene lauryl ether (EO = 9), 1.0% by weight of polyhydroxyethylene cetyl stearyl ether (Cetomacrogol 1000), 2.5% by weight of viscous paraffin oil / Capric acid ester (Cetiol LC), 3.0% by weight of polyethylene glycol 400, and deionized water were mixed (100% by weight of the whole composition) to prepare a patch.
Claims (12)
A cosmetic composition characterized by comprising an extract of Hong Sung Tea, a fermented Hongdong tea extract or a mixture thereof as an active ingredient.
상기 발효된 홍설차 추출물은 홍설차를 버섯, 유산균, 젖산균 및 효모로 이루어진 군에서 선택된 1종 이상으로 발효하여 얻은 추출물인 것을 특징으로 하는 화장료 조성물.
The method according to claim 1,
The cosmetic composition according to any one of claims 1 to 3, wherein the fermented extract is obtained by fermentation of at least one selected from the group consisting of mushroom, lactic acid bacteria, lactic acid bacteria and yeast.
상기 버섯은 송이버섯, 치마버섯, 차가버섯, 잎새버섯 또는 이의 혼합된 균주인 것을 특징으로 하는 화장료 조성물.
3. The method of claim 2,
Wherein the mushroom is selected from the group consisting of matsutake mushroom, skirt mushroom, chaga mushroom, mushroom mushroom, or a mixed strain thereof.
상기 발효는 10 ℃ 내지 35 ℃에서 5일 내지 40일 동안 실시하는 것을 특징으로 하는 화장료 조성물.
3. The method of claim 2,
Wherein the fermentation is carried out at 10 占 폚 to 35 占 폚 for 5 days to 40 days.
상기 유효성분은 전체 조성물 총 중량 대비 0.0001 중량% 내지 30 중량%인 것을 특징으로 하는 화장료 조성물.
The method according to claim 1,
Wherein the active ingredient is 0.0001% by weight to 30% by weight based on the total weight of the composition.
항산화, 보습 또는 주름 개선인 것을 특징으로 하는 화장료 조성물.
The method according to claim 1,
Antioxidant, moisturizing, or wrinkle improvement.
Wherein the extract is a fermented red ginseng extract or a mixture thereof as an active ingredient.
상기 발효된 홍설차는 홍설차를 버섯, 유산균, 젖산균 및 효모로 이루어진 군에서 선택된 1종 이상으로 발효하여 얻은 것을 특징으로 하는 피부 외용제.
8. The method of claim 7,
Characterized in that the fermented red tea is obtained by fermenting red ginseng root with at least one member selected from the group consisting of mushroom, lactic acid bacteria, lactic acid bacteria and yeast.
상기 버섯은 송이버섯, 치마버섯, 차가버섯, 잎새버섯 또는 이의 혼합된 균주인 것을 특징으로 하는 피부 외용제.
9. The method of claim 8,
Wherein said mushroom is selected from the group consisting of matsutake mushroom, skirt mushroom, chaga mushroom, mushroom mushroom, or a mixture thereof.
상기 발효는 10 ℃ 내지 35 ℃에서 5 내지 40일 동안 실시하는 것을 특징으로 하는 피부 외용제.
9. The method of claim 8,
Wherein the fermentation is carried out at 10 캜 to 35 캜 for 5 to 40 days.
상기 유효성분은 전체 조성물 총 중량 대비 0.0001 중량% 내지 30 중량%인 것을 특징으로 하는 피부 외용제.
8. The method of claim 7,
Wherein the active ingredient is 0.0001% by weight to 30% by weight based on the total weight of the composition.
항산화, 보습 또는 주름 개선인 것을 특징으로 하는 피부외용제.The method according to claim 1,
Antioxidant, moisturizing, or wrinkle improvement.
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| Application Number | Priority Date | Filing Date | Title |
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| KR1020140065996A KR20150137692A (en) | 2014-05-30 | 2014-05-30 | Cosmetic composition comtaining Lethariella cladonioides extracts |
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| KR1020140065996A KR20150137692A (en) | 2014-05-30 | 2014-05-30 | Cosmetic composition comtaining Lethariella cladonioides extracts |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106074263A (en) * | 2016-07-05 | 2016-11-09 | 上海相宜本草化妆品股份有限公司 | A kind of book on Chinese herbal medicine extract and the application in cosmetics thereof |
| CN107714546A (en) * | 2017-11-02 | 2018-02-23 | 广州丽丰化妆品制造有限公司 | A kind of toothpaste and its production method for suppressing helicobacter pylori in oral cavity |
| CN111067851A (en) * | 2020-01-17 | 2020-04-28 | 云南白药集团股份有限公司 | Preparation method of tricholoma matsutake mask liquid |
-
2014
- 2014-05-30 KR KR1020140065996A patent/KR20150137692A/en not_active Application Discontinuation
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106074263A (en) * | 2016-07-05 | 2016-11-09 | 上海相宜本草化妆品股份有限公司 | A kind of book on Chinese herbal medicine extract and the application in cosmetics thereof |
| CN106074263B (en) * | 2016-07-05 | 2020-08-07 | 上海相宜本草化妆品股份有限公司 | Herbal extract and application thereof in cosmetics |
| CN107714546A (en) * | 2017-11-02 | 2018-02-23 | 广州丽丰化妆品制造有限公司 | A kind of toothpaste and its production method for suppressing helicobacter pylori in oral cavity |
| CN107714546B (en) * | 2017-11-02 | 2020-12-29 | 广州丽丰化妆品制造有限公司 | Toothpaste capable of inhibiting helicobacter pylori in oral cavity and production method thereof |
| CN111067851A (en) * | 2020-01-17 | 2020-04-28 | 云南白药集团股份有限公司 | Preparation method of tricholoma matsutake mask liquid |
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