KR20110126520A - Cosmetic composition comprising extract of ginseng fermented by phellinus linteus or ganoderma lucidum - Google Patents

Abstract

PURPOSE: A cosmetic composition containing extract of fermented Phellinus linteus and Ganoderma lucidum is provided to ensure antioxidation and collagen synthesis. CONSTITUTION: A cosmetic composition contains extract of fermented Phellinus linteus or Ganoderma lucidum or mixture thereof as an active ingredient. The extract is contained in 0.0001-30 wt% based on total weight. The cosmetic composition is manufactured in the form of an external use skin ointment, lotion, softener, nutrition cream, massage cream, essence, pack, emulsion, or oil gel. The cosmetic composition further contains oil, water, surfactant, moisturizing agent, low alcohol of 1-4 carbon atoms, chelating agent, pigment, antiseptic, or perfume.

Description

Cosmetic composition comprising an extract of ginseng fermented with a situation mushroom or Ganoderma lucidum mushroom {{Cosmetic composition comprising extract of ginseng fermented by Phellinus linteus or Ganoderma lucidum}

The present invention relates to a cosmetic composition comprising an extract of ginseng as an active ingredient.

Skin is an important organ that plays a variety of physiological functions such as barrier function, temperature control, and excretion that protect the human body from the external environment and prevent internal moisture and useful components from leaking out. However, due to the following reasons, the activity of the skin cells is reduced, changes in the appearance of blemishes, freckles and blotch, etc., and the aging phenomenon that the skin becomes dull and rough.

First, collagen present in the dermal layer of the skin plays an important role in the components of the skin, such as mechanical firmness of the skin, resistance of connective tissue, adhesion of tissue, support of cell adhesion. Such collagen is reduced by photoaging due to stress, aging or ultraviolet irradiation, resulting in loosening of skin tissue and loss of elasticity.

Second, inflammatory reactions may be caused by external factors such as stress or ultraviolet irradiation, or the activity of immune cells may be reduced by aging, resulting in improper regulation of skin immune function, thereby promoting skin aging.

Third, the cell function of the epidermis is reduced, metabolic action is not smooth, the exfoliation does not occur well, the ultraviolet rays in the state of excessive accumulation of keratin decreases the elasticity and causes the occurrence of wrinkles.

Fourth, when the sebaceous film is not formed on the epidermis, moisture and sebum secretion are reduced, and the skin is dried and wrinkles are formed.

Fifth, the oxygen radicals generated in subcutaneous fat destroy cell membranes and cell nuclei, thereby lowering blood circulation, resulting in lack of skin nutrition and moisture, thereby promoting skin aging.

Various studies are being conducted to improve the wrinkles and delay the skin aging as described above. As the aging society enters the consumer's desire to have young and elastic skin, and the Cosmetics Act of July 2000 was implemented. Accordingly, research and development of functional cosmetics for improving skin wrinkles or delaying skin aging have become more active. Such functional cosmetics may use natural substances such as herbal medicines in order not to cause toxicity or irritation to the skin. For example, liquorice, golden, salvia, etc. Aloe, horse chestnut, and the like which have UV-protective functions, such as cordyceps, plantain, aloe, sewage, etc., which have skin regeneration effects, are known.

Among the natural materials, ginseng has been frequently used as a cosmetic by improving the skin wrinkles and exerting a whitening effect as well as physiological activity, as well as immune and anti-cancer effects, but its effects are insignificant and do not last long, so its use is limited.

The technical problem to be achieved by the present invention is to provide a cosmetic composition excellent in antioxidant effect, skin wrinkle improvement effect and whitening effect and long-lasting effect compared to the conventional cosmetic composition.

In order to achieve the above object of the present invention, the present invention provides a cosmetic composition comprising an extract of ginseng fermented with a situation mushroom, an extract of ginseng fermented with Ganoderma lucidum mushroom or a mixture thereof as an active ingredient .

The inventors of the present invention while trying to solve the problem that the skin wrinkle improvement effect and the whitening effect is insignificant when applying the conventional cosmetic composition comprising ginseng or ginseng extract on the skin, the extract of ginseng fermented with the situation mushroom in the cosmetic composition , When the extract of ginseng fermented with Ganoderma lucidum mushrooms or a mixture thereof in a certain amount to confirm that not only effectively improve the skin wrinkles but also excellent skin whitening effect was completed the present invention.

Therefore, when the cosmetic composition of the present invention is applied to the skin, it exhibits a better skin wrinkle improvement effect than the conventional single ginseng extract, situation mushroom extract, or ganoderma lucidum extract, and significantly inhibits melanin synthesis, thus excellent skin whitening effect. .

The extract of ginseng fermented with a situation mushroom of the present invention, the extract of ginseng fermented with Ganoderma lucidum mushroom or a mixture thereof is included in 0.0001 to 30% by weight relative to the total weight of the cosmetic composition, 0.005 to relative to the total weight of the cosmetic composition based on the embodiment It is preferably included in 10% by weight, more preferably contained in 0.01 to 5% by weight. If the content is less than 0.0001% by weight it is difficult to expect a substantial skin wrinkle improvement effect, if the content exceeds 30% by weight may cause a problem that the manufacturing cost is too high compared to the cosmetic effect.

Ginseng (Ginseng Radix) according to the present invention is the root of perennial herbaceous ginseng (Panax ginseng) belonging to the family Arboraceae, Phellinus linteus according to the present invention is a mushroom belonging to the basidiomycetes mud mushroom family, Ganoderma lucidum (Ganoderma lucidum) according to the present invention is a mushroom belonging to the genus Fungi (Basidiomycetes).

Ginseng fermented with ginseng or Ganoderma lucidum fermented with a situation mushroom according to the present invention means that after the culture of ginseng inoculated with mycelium of situation mushroom or Ganoderma lucidum mushroom, preferably the situation mushroom or Ganoderma lucidum fermented to the inside of ginseng do. As ginseng, whole ginseng, shredded ginseng or powdered ginseng may be used, and the present invention is not limited to the form of ginseng illustrated above. The fermentation is preferably made for 20 to 40 days at 10 to 35 ℃, the time required for the fermentation may vary depending on the fermentation temperature. In addition, since the fermentation conditions may vary depending on the state of ginseng, situation mushroom or Ganoderma lucidum used in the present invention, the content of the present invention is not limited to the fermentation temperature and fermentation time illustrated above.

In addition, the extract of the ginseng fermented with the situation mushroom of the present invention or the extract of ginseng fermented with Ganoderma lucidum means a substance that is dissolved in a solvent to dissolve the ginseng fermented with ginseng or Ganoderma lucidum in the solvent. Water may be used as a solvent at this time; Lower alcohols having 1 to 4 carbon atoms such as methanol, ethanol, propyl alcohol, and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether and dichloromethane may be used alone or in combination of two or more thereof.

The formulation of the cosmetic composition of the present invention is not particularly limited and may be appropriately selected as desired. For example, the cosmetic composition of the present invention may be prepared in the form of external skin ointment, lotion, supple cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, pack, emulsion, oil gel and the like.

The skin external ointment is 55-97% by weight of petrolatum and 0.1-5% by weight of polyoxyethylene oleyl-ether phosphate in addition to the extract of ginseng fermented with the situation mushroom of the present invention, the extract of ginseng fermented with Ganoderma lucidum or a mixture thereof It can be manufactured so that it contains.

The flexible softener is 1 ~ 10% by weight of polyhydric alcohols such as propylene glycol, glycerin and polyoleyl ether, polyoxy in addition to the extract of ginseng fermented with the situation mushroom of the present invention, the extract of ginseng fermented with Ganoderma lucidum or a mixture thereof It can be prepared to contain 0.05 to 2% by weight of the surface active agent such as ethylene hardened castor oil.

The nutritious longevity or nourishing cream is an extract of ginseng fermented with the situation mushroom of the present invention, in addition to the extract of ginseng fermented with Ganoderma lucidum mushrooms or mixtures thereof, oils such as squalane, petrolatum, octyldodecanol, and 5-20 wt% It may be prepared to contain 3 to 15% by weight of waxes such as tanol, stearyl alcohol and beeswax.

The massage cream contains 30 to 70% by weight of an oil such as liquid paraffin, petrolatum, isononyl isononanoate in addition to the extract of ginseng fermented with the situation mushroom of the present invention, the extract of ginseng fermented with Ganoderma lucidum mushroom, or a mixture thereof Can be prepared.

The essence may be prepared to contain 5 to 30% by weight of polyhydric alcohols such as propylene glycol, glycerin in addition to the extract of ginseng fermented with the situation mushroom of the present invention, the extract of ginseng fermented with Ganoderma lucidum mushrooms or mixtures thereof.

The pack is a peel off pack or general emulsion type containing 5 to 20% by weight of polyvinyl alcohol in addition to the extract of ginseng fermented with the situation mushroom of the present invention, the extract of ginseng fermented with Ganoderma lucidum or a mixture thereof The cosmetic may be prepared in a wash off pack containing 5 to 30% by weight of pigments such as kaolin, talc, zinc oxide and titanium dioxide.

In addition, the cosmetic composition of the present invention, in addition to the components described above in each formulation, oil, water, surfactants, humectants, lower alcohols having 1 to 4 carbon atoms, thickeners, chelating agents, pigments, preservatives or A fragrance etc. can be mix | blended suitably as needed and can be used.

The cosmetic composition of the present invention can be used for antioxidant of the skin, skin wrinkle improvement, skin elasticity improvement or skin whitening improvement.

The cosmetic composition of the present invention is excellent in antioxidant effect, collagen synthesis effect and melanin synthesis inhibitory effect, while significantly improving skin wrinkles and whitening of the skin, while not causing allergic reactions or irritation to the skin, and thus it is safe for human skin. Therefore, the cosmetic composition of the present invention can be prepared in various forms of skin ointment, lotion, supple cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, pack, emulsion, oil gel and the like.

Hereinafter, examples and the like will be described in detail to help understand the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art.

Example 1 Extract of Ginseng Fermented with Situary Mushroom

Phellinus linteus as a situation mushroom strain was incubated in a PDA (potato dextrose agar) solid medium at a temperature of 25 ℃. The cultured solid bacteria were inoculated in PDB (potato dextrose broth) liquid medium and shaken at 25 ° C. and 130 rpm until the spore concentration became 10 ⁸ CFU / mL. Then, 150 μl of the cultured spawn was injected into ginseng with a 1 mL disposable syringe, and then protected at 24 ° C. and 85 to 90% humidity for one week, and 20 ° C. and 85 to 90% humidity until fruiting body was completed. Stored at. The mycelium of the situation mushroom was penetrated to the inside of ginseng to carry out the fermentation for 20 to 40 days while maintaining the temperature of 10 ~ 35 ℃ to ferment to the inside of the ginseng as a whole to prepare the ginseng fermented with the situation mushroom.

After drying the ginseng fermented with the prepared situation mushrooms and powdered by 200 g of powder, the extract solvent mixed with distilled water and ethanol 3: 7 was extracted and filtered and dried under reduced pressure of 70% ethanol extract. It was. As a result, 37 g of ginseng extract fermented with the situation mushroom was obtained.

Example 2 Extract of Ginseng Fermented with Ganoderma Lucidum Mushroom

Using the same method as in Example 1, using the Ganoderma lucidum instead of situation mushrooms (using Ganoderma lucidum as Ganoderma lucidum strain) to obtain 34 g of the extract of ginseng fermented with Ganoderma lucidum.

<Comparative Example 1>: situation mushroom extract

After powdering 200 g of mushrooms and powdering them, the mixture was extracted and mixed with distilled water and ethanol 3: 7, and 70% ethanol extract obtained by filtration was dried under reduced pressure to obtain 23 g of mushroom mushrooms.

<Comparative Example 2>: Ganoderma lucidum extract

Using the same method as Comparative Example 1, using the Ganoderma lucidum instead of situation mushrooms to obtain 26g of Ganoderma lucidum extract.

Comparative Example 3 Ginseng Extract

Using the same method as Comparative Example 1, 32 g of ginseng extract was obtained using ginseng instead of situation mushroom.

(Cytotoxicity test)

In order to verify the safety of the extract of ginseng fermented with the situation mushroom of Example 1 and the extract of ginseng fermented with Ganoderma lucidum of Example 2, V79-4 cells (Chinese hamster, lung tissue fiber) for each extract A continuous cell line of blast cells) was cultured to perform cytotoxicity by MTT assay, and the results are shown in Table 1 below. Cytotoxicity test was carried out in Comparative Examples 1 to 3, squalane and sodium lauryl sulfate (SLS) in the same manner.

sample IC ₅₀ (%, w / v) Example 1 (extract of ginseng fermented with the situation mushroom) 2.0 Example 2 (extract of ginseng fermented with Ganoderma lucidum mushroom) 2.5 Comparative Example 1 (Situation Mushroom Extract) 0.5 Comparative Example 2 (Ganoderma lucidum extract) 0.5 Comparative Example 3 (Ginseng Extract) 1.0 Squalane 0.05 Sodium Lauryl Sulfate 0.005 IC ₅₀ (Inhibitory Concentration 50)-50% cell killing concentration

From Table 1, the extract used in the cosmetic composition (Examples 1 and 2 and Comparative Examples 1 to 3) showed about 20 times lower cytotoxicity than squalane known as an oil-soluble raw material with excellent safety, and is a surfactant sodium About 200 times lower cytotoxicity than lauryl sulfate, it was confirmed that the safety is excellent.

In particular, the extract (Examples 1 to 2) used in the cosmetic composition of the present invention shows a cytotoxicity about 2 times lower than the extracts used in the conventional cosmetic composition (Comparative Examples 1 to 3), the extract of the present invention is It was found to be safer than conventional extracts.

(Allergy assessment (LLNA))

As an experiment for confirming whether the extract of the present invention (Examples 1 to 2) allergens were identified as safe in the cytotoxicity test, an allergic reaction was performed using a mixed solvent in which acetone and olive oil were mixed at 4: 1. Induced local lymph node assay (LLNA) assay was performed (Kimber, J Appl Toxicol 10, 173-180, 1990).

First, 20%, 40%, and 60% of the extract was applied to both ears of rats (Balb / c) for 50 days for 3 days, and then auricular lymph nodes were isolated from the mice. The isolated lymph nodes were pulverized into a single cell state, incubated for 24 hours with the addition of radioisotope [ ³ H] -methyl thymidine, and then beta -scintillation counter (Beckman LS 6000 TA, USA). Dpm (disintegraion per minute) and amplification degree of cell proliferation were measured using Table 3 and the results are shown in Table 2 below. The mixed solvent was used as a control.

division density(%) Lymph node count
(Cell / mL * 10 ⁷ ) dpm ¹ (average) SI ² (amplitude) Acetone: Olive Oil (4: 1) 1.70 2870 Transportation Example 1
(Fermented with situation mushroom
Extract of ginseng) 20 1.71 2296 0.8 40 2.04 3157 1.1 60 3.23 4592 1.5 Example 2
(Fermented with Ganoderma lucidum mushroom
Extract of ginseng) 20 1.72 2880 1.0 40 2.00 3017 1.1 60 3.12 4489 1.6 1. dpm-the amount of [ ³ H] -methylthymidine inserted into the cell
2. SI (stimulation index)-the average dpm of the sample divided by the average dpm of the control

The allergy evaluation result indicates the degree of lymphocyte amplification of the sample group with respect to the control group, and if the amplification degree (SI) of the sample is 3 or more at any concentration and shows a tendency to increase in a concentration-dependent manner, it is regarded as an allergen. As shown, the extract used in the cosmetic composition of the present invention was shown to have an amplification degree (SI) of 2 or less, which is not allergenic.

(Free radical scavenging ability)

Free radical scavenging ability of the extracts of Examples 1 to 2 and Comparative Examples 1 to 3 was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method (Blois, Nature 181, 1190, 1958). . DPPH is a relatively stable free radical which, when present in the radical state, exhibits maximum absorption at 517 nm and loses its absorbance when radicals are scavenged. DPPH was used by Sigma Co., Ltd. and dissolved in methyl alcohol at a concentration of 0.15 mM.

First, 100 μl of the extract and vitamin C were placed in each well of a 96-well plate. 100 μl each of the DPPH solution was added thereto, followed by standing at room temperature for 30 minutes, and the absorbance at 517 nm was measured using a Micro plate reader (BioTek EL-340). At this time, methyl alcohol was used as a control.

The concentration of the extract when the absorbance of the sample was half the absorbance of the control group was expressed as IC ₅₀ (Inhibitory Concentration 50), and the results are shown in Table 3 below.

sample IC ₅₀ (%, w / v) Example 1 (extract of ginseng fermented with the situation mushroom) 0.0007 Example 2 (extract of ginseng fermented with Ganoderma lucidum mushroom) 0.0006 Comparative Example 1 (Situation Mushroom Extract) 0.0025 Comparative Example 2 (Ganoderma lucidum extract) 0.002 Comparative Example 3 (Ginseng Extract) 0.001 Vitamin c 0.0005 Control group 0.0025

As shown in Table 3, the extract used in the cosmetic composition of the present invention showed a free radical scavenging ability to a similar extent as vitamin C known as a free radical scavenger, indicating that the free radical scavenging ability was very strong.

In particular, it can be seen that the extract (Examples 1 to 2) used in the cosmetic composition of the present invention was significantly superior in free radical scavenging ability than the extracts (Comparative Examples 1 to 3) used in the conventional cosmetic composition.

Collagen synthesis ability evaluation

Experiments were performed to evaluate the collagen synthesis ability of the extracts of Examples 1 to 2 and Comparative Examples 1 to 3. Biosynthetic collagen measurements were quantified using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay KIT).

First, the extract is added to the culture medium of human-derived fibroblasts so that the final concentration is 10 ㎍ / mL, vitamin C is added so that the final concentration is 52.8 ㎍ / mL, each cultured for 2 days, and then the culture solution The collagen biosynthesis was analyzed at each level using the PICP EIA Kit. No addition culture was used as a control. Collagen synthesis capacity was calculated as a percentage of collagen synthesis relative to the non-added culture, and the results are shown in Table 4 below.

sample Collagen synthesis rate (%) Example 1 (extract of ginseng fermented with the situation mushroom) 139 Example 2 (extract of ginseng fermented with Ganoderma lucidum mushroom) 130 Comparative Example 1 (Situation Mushroom Extract) 110 Comparative Example 2 (Ganoderma lucidum extract) 107 Comparative Example 3 (Ginseng Extract) 112 Vitamin c 125 Control group -

From the results of Table 4, the extract used in the cosmetic composition of the present invention (Examples 1 to 2) for the cultured human-derived fibroblasts was superior to the collagen synthesis ability than vitamin C known as an essential nutrient for collagen synthesis, In particular, it can be seen that it has a significantly superior collagen synthesis ability compared to the extract used in the conventional cosmetic composition (Comparative Examples 1 to 3).

(Skin irritation evaluation)

Patch test using a guinea pig (Guinea Pig) for the skin irritation test of the extracts of Examples 1 to 2 [Reference: ① Draize, J.H. (1959), Dermal toxicity. Assoc. Food and Drug officials, US. Appraisal of the safety of chemicals in Food, Drugs and Cosmetics., 46-59, Texas State Dept of Health, Austin, Texas; ② Federal Register (1973), Method of testing primary irritant substances 38 (187), 1500-1541].

First, it was adapted to the environment for 24 hours to remove the back hair, which is the sample application site, and to minimize skin irritation. Then, the sample application site was set (1.5 cm × 1.5 cm) and each of the above extracts and gauzes were applied, and then sealed with a foil of solid material to prevent evaporation and loss of the sample, and for 48 hours with an elastic bandage. Fixed. Then, the degree of stimulation immediately after removing the closed patch and 24 hours (48 hours, 72 hours after the patch) was determined, and the stimulation index (PII: primary cutaneous irritation index) and the stimulus degree are shown in Table 5 below. As a control, guinea pigs with purified water and gauze were used.

division density(%) Stimulus Index (PII) Irritation degree Control group 0.50 Microstimulation Example 1
(Extract of Ginseng Fermented with Situation Mushroom) 0.5 0.51 Microstimulation 5.0 0.48 Microstimulation 50.0 0.47 Microstimulation Example 2
(Extract of Ginseng Fermented with Ganoderma Lucidum Mushroom) 0.5 0.52 Microstimulation 5.0 0.47 Microstimulation 50.0 0.48 Microstimulation

As shown in Table 5, the extract used in the cosmetic composition of the present invention was confirmed to be very safe for the skin.

(Melanin Synthesis Inhibition Evaluation)

In order to evaluate the melanin synthesis inhibitory ability of the extracts of Examples 1 and 2 and Comparative Examples 1 to 3, the following test was performed using melanocytes, which are melanocyte synthetic cells.

First, the extract is added to the melanocyte culture solution to a final concentration of 10 μg / mL, and arbutin is added to a final concentration of 1 mg / mL, and then collected by centrifugation at 1,500 g for 5 minutes and washed with PBS buffer. It was. Then extract with 200 μl of extraction buffer (1% Nonidet P-40, 0.01% SDS, 0.1M Tris-HCl pH 7.2, protease inhibitor cocktail) for 1 hour at 4 ° C and centrifuge for 5 minutes at 15,000 rpm. Separated. The precipitate obtained by centrifugation was treated with 1N NaOH to dissolve the melanin and transferred to a 96-well plate, followed by measurement of absorbance at 480 nm using an ELISA reader (PowerWave X340, Bio-tech Instruments, Inc.). The amount was determined. In addition, the total protein amount of each well was quantified by a BCA ^TM (Bicinchoninic acid) assay kit, and the amount of melanin synthesized was corrected by the total protein amount. No addition culture was used as a control.

The test was repeated three times for each sample, and the melanin synthesis inhibitory ability was calculated by calculating the amount of melanin synthesis relative to the control medium without a control as a percentage of synthesis inhibition and the results are shown in Table 6 below.

sample Melanin synthesis inhibition rate (%) Example 1 (extract of ginseng fermented with the situation mushroom) 31.5 Example 2 (extract of ginseng fermented with Ganoderma lucidum mushroom) 28.4 Comparative Example 1 (Situation Mushroom Extract) 19.2 Comparative Example 2 (Ganoderma lucidum extract) 15.9 Comparative Example 3 (Ginseng Extract) 16.7 Arbutin 39.5 Control group -

From the results of Table 6, the extract used in the cosmetic composition of the present invention (Examples 1 to 2) with respect to the cultured melanocytes of the mice, effectively inhibited melanin synthesis, in particular Example 1 to inhibit melanin synthesis It has been shown to be able to inhibit melanin synthesis to a similar degree as arbutin. From these results, it can be seen that the extracts (Examples 1 to 2) used in the cosmetic composition of the present invention have better melanin synthesis inhibiting ability than the extracts (Comparative Examples 1 to 3) used in the conventional cosmetic composition. .

<Examples 3 to 5 and Comparative Example 4> Preparation of oil-in-water emulsion

Oil-in-water emulsion containing the mixture of the extract of Example 1 and the extract of Example 2 in a ratio of 1: 1 (Examples 3 to 5), oil-in-water emulsion containing the extract of Example 1 (Example 6) and an oil-in-water emulsion (Example 7) including the extract of Example 2 were prepared. In addition, an oil-in-water emulsion not containing the extract was prepared as Comparative Example 4. Detailed compositions (% by weight) are shown in Table 7 below.

division Example
3 Example
4 Example
5 Example
6 Example
7 Comparative example
4


Awards Purified water Balance Balance Balance Balance Balance Balance Triethanolamine 0.15 0.15 0.15 0.15 0.15 0.15 Carboxy vinyl polymer ¹ 15 15 15 15 15 15 glycerin 6 6 6 6 6 6 Propylene glycol 4 4 4 4 4 4 Paraoxybenzoic Acid Ester 0.2 0.2 0.2 0.2 0.2 0.2 Extract of Example 1 0.005 0.5 2.5 2.5 - - Extract of Example 2 0.005 0.5 2.5 - 2.5 -



Paid Cetostearyl alcohol One One One One One One Glyceryl Monostearate One One One One One One Sorbitan monosulfate ² One One One One One One POE (20) sorbitan mono
Stearate 1.5 1.5 1.5 1.5 1.5 1.5 Paraoxybenzoic acid propyl 0.1 0.1 0.1 0.1 0.1 0.1 Isocetil Myristate 5 5 5 5 5 5 Isocetyloctanoate ³ 5 5 5 5 5 5 Squalane 5 5 5 5 5 5 Spices Quantity Quantity Quantity Quantity Quantity Quantity 1. Carboxy vinyl polymer-1 wt% aqueous solution of Carbopol 941 from BFGoodrich, USA
2. Monostearate sorbitan-Arlacel 60 between British Isia (ICI)
3. Isocetyl Octanoate-ICEH, Nihon KoKyu Alcohol, Japan

(Wrinkle improvement effect)

The oil-in-water emulsions of Examples 3 to 7 and Comparative Example 4 were measured for the effects of wrinkle improvement on the healthy 35 to 50 year old women as follows.

After dividing 40 women between 35 and 50 years old into 6 groups of 10 people, 5 groups were applied the oil-in-water emulsions of Examples 3 to 7 to the face once a day for 3 months, and the other 1 group was compared. The oil-in-water emulsion of Example 4 was applied to the face part once a day for 3 months.

After 3 months, the improvement of wrinkles was evaluated by questionnaire and image analysis of wrinkles. The subject's questionnaire was judged in three stages of no improvement, slight improvement, and significant improvement in comparison with before use regarding wrinkle improvement and elasticity enhancement, and the results are shown in Table 8 below.

division No improvement Minor improvements A significant improvement Example 3 One 8 One Example 4 2 5 3 Example 5 One 6 3 Example 6 One 7 2 Example 7 One 7 2 Comparative Example 4 8 2 0

As shown in Table 8, it was confirmed that the oil-in-water emulsions of Examples 3 to 7 of the present invention has an excellent wrinkle improvement effect compared to the oil-in-water emulsion of Comparative Example 4.

Example 6 Preparation of Lotion

Extract of ginseng fermented with S. mushrooms, extract of ginseng fermented with Ganoderma lucidum mushrooms, or mixtures thereof 0.1 wt%, glycerin 5.0 wt%, dipropylene glycol 3.0 wt%, hyaluronic acid 0.5 wt%, polyoxyethylene hydrogenated castor oil 0.1 A skin lotion was prepared by mixing a weight%, polyethylene oleyl ether 0.1 weight%, ethanol 5.0 weight%, preservative 0.15 weight%, an appropriate amount of perfume and colorant and the remaining purified water.

Example 7 Preparation of Essence

Extract of ginseng fermented with S. mushrooms, extract of ginseng fermented with Ganoderma lucidum mushrooms, or mixtures thereof 7.0 wt%, cetostearyl alcohol 1.0 wt%, self-emulsifying monostearic acid 1.0 wt%, beeswax 0.5 wt%, squalane 5.0 wt% %, Isocetyloctanoate 3.0 wt%, dimethylsiloxane 0.3 wt%, monostearate sorbitan 0.5 wt%, monostearate polyethylene glycol 8.0 wt%, glycerin 4.0 wt%, propylene glycol 0.2 wt%, carboxypolymer 0.22 wt%, Essence was prepared by mixing 0.25% by weight of triethanolamine, an appropriate amount of preservative, a flavoring agent, and a pigment with a residual amount of purified water.

Example 8 Lotion Preparation

Extract of ginseng fermented with S. mushrooms, extract of ginseng fermented with Ganoderma lucidum mushroom, or mixtures thereof 5.0% by weight, cetostearyl alcohol 0.8% by weight, 1.0% by weight of self-emulsifying monostearic acid, 0.5% by weight of beeswax, 0.5% of stearic acid %, Liquid paraffin 7.0 wt%, squalane 5.0 wt%, macadamia oil 3.0 wt%, isocetyloctanoate 2.0 wt%, dimethylsiloxane 0.3 wt%, monostearate sorbitan 0.5 wt%, monostearate polyethylene glycol 1.2 wt% %, Glycerin 4.0 wt%, Propylene glycol 4.0 wt%, Betaine 4.0 wt%, Carboxypolymo 0.12 wt%, Triethanolamine 0.15 wt%, Preservative 0.25 wt%, Proper amount of fragrances, pigments and remaining purified water mixed Was prepared.

Example 9 Cream Preparation

Extract of ginseng fermented with Situary mushroom, extract of ginseng fermented with Ganoderma lucidum mushroom or mixture thereof 7.0 wt%, cetostearyl alcohol 3.0 wt%, self-emulsifying monostearic acid 0.5 wt%, lipophilic monostearic acid 1.5 wt%, 0.5% by weight of beeswax, 8.0% by weight of liquid paraffin, 7.0% by weight of squalane, 4.0% by weight of isocetyloctanoate, 4.0% by weight of purified jojoba oil, 0.3% by weight of dimethylsiloxane, 1.0% by weight of sorbitan monostearate, polyethylene glycol monostearate 1.2% by weight, glycerin 6.0% by weight, propylene glycol 4.0% by weight, betaine 4.0% by weight, xanthan gum 0.06% by weight, triethanolamine 0.10% by weight, preservative 0.25% by weight, a suitable amount of fragrances, pigments and remaining purified water Cream was prepared.

Example 10 Gel Preparation

Extract of ginseng fermented with S. mushrooms, extract of ginseng fermented with Ganoderma lucidum mushrooms, or mixtures thereof 1.0 wt%, glycerin 4.0 wt%, propylene glycol 4.0 wt%, ethanol 10 wt%, polyoxyethylene hydrogenated castor oil 0.1 wt% , 0.30% by weight of a carboxypolymer, 0.30% by weight of triethanolamine, an appropriate amount of preservatives, fragrances and pigments and a residual amount of purified water were mixed to prepare a gel.

Claims (5)

A cosmetic composition comprising an extract of ginseng fermented with a situation mushroom, an extract of ginseng fermented with Ganoderma lucidum mushroom, or a mixture thereof as an active ingredient. The cosmetic composition according to claim 1, wherein the extract of ginseng fermented with the situation mushroom, the extract of ginseng fermented with Ganoderma lucidum mushroom, or a mixture thereof is 0.0001 to 30% by weight based on the total weight of the cosmetic composition. According to claim 1, wherein the cosmetic composition is an external skin ointment, lotion, supple cosmetics, nourishing cosmetics, nourishing cream, massage cream, essence, pack, emulsion and oil gel, characterized in that any one selected from the group consisting of. Cosmetic composition. The method of claim 1, further comprising at least one additive selected from the group consisting of oils, water, surfactants, humectants, lower alcohols having 1 to 4 carbon atoms, thickeners, chelating agents, pigments, preservatives and flavoring agents. Cosmetic composition. The cosmetic composition according to any one of claims 1 to 4, wherein the cosmetic composition is for antioxidant of the skin, for improving skin wrinkles, for improving skin elasticity, or for improving skin whitening.