KR20190017420A - Cosmetic compositions for improving skin moisturizing - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin moisturization, including a specific protein mixture and a spirulina extract. The present invention is capable of making continuous moisturizing performance long-lasting without irritating skin.

Description

COSMETIC COMPOSITIONS FOR IMPROVING SKIN MOISTURIZING [0002]

The present invention relates to a moisturizing cosmetic composition comprising a mixture of specific proteins and Spirulina extract.

The skin is largely divided into three layers of epidermis, dermis and subcutaneous tissue in order from the outside. It protects the internal organs from external stimuli such as temperature, humidity change, ultraviolet rays, air pollutants and chemicals. Skin, in particular, plays an important role in controlling the evaporation of about 70% of the human body's water. Moisture transports various physiologically active substances necessary for the human body, and helps to keep the skin soft and moist.

The epidermis is divided into the stratum corneum, the granular layer, the superficial layer and the basal layer in the order from the outside. The cells of the stratum corneum act like bricks, and the intercellular lipids between the keratinocytes function as the mortar to form the skin barrier Invest., Dermatol 80 (Suppl.), 44-49, 1983). Generally, keratinocytes distributed in human epidermis have high concentration of natural moisturizing factor and help to retain moisture of skin. However, due to such factors as the living environment, stress, environmental pollution, aging, etc., the moisture of the stratum corneum decreases and the skin becomes dry and the surface of the skin becomes rough. do.

Conventionally, the moisturizing agent has been used in a manner of absorbing moisture or preventing evaporation of moisture, thereby increasing water retention in the stratum corneum. However, the moisturizing agent can not maintain a long-lasting moisturizing performance, come.

On the other hand, the incidence of various environmental skin diseases such as atopic dermatitis and contact dermatitis is recently increasing rapidly. Atopic dermatitis is a syndrome of dermatophytosis that manifests as a complex manifestation of symptoms such as strong cannibalism, dryness, severe keratinization, skin cracking, and erythema of unknown origin. It usually starts in infancy and appears in many infants, children and adolescents. Skin having an environmental skin disease such as atopic dermatitis not only needs to continuously moisturize, but also heals skin and improves skin. In Korean Patent No. 10-0404072, a method of applying a lipid complex of ceramides for restoring moisture and skin barrier or applying a steroid preparation has been used, and its use is limited due to problems such as lowering of skin safety or side effects.

Therefore, there is a need for research and development of a moisturizing cosmetic composition capable of imparting effective healing and skin improving performance to environmental skin diseases such as atopic dermatitis, which is rapidly increasing with the improvement of the moisturizing performance to the skin.

Korean Patent Publication No. 10-0404072 (Oct. 21, 2003)

It is an object of the present invention to provide a cosmetic composition for moisturizing which can feel a sense of sheen from the skin and is excellent in feeling of use, has no irritation to the skin, and can realize long-lasting moisturizing effect.

It is another object of the present invention to provide a moisturizing cosmetic composition useful for skin improvement such as skin anti-allergy, anti-inflammation, skin barrier function enhancement, and at the same time, in addition to the above-described moisturizing function, healing of environmental skin diseases such as atopic dermatitis.

According to an aspect of the present invention,

The present invention provides a moisturizing cosmetic composition comprising a protein mixture containing an immunoglobulin, an epidermal growth factor receptor (EGFR) and a lactoferrin, and a Spirulina extract.

In the cosmetic composition for moisturizing according to an embodiment of the present invention, the protein mixture may contain 20 to 50 wt%, epidermal growth factor receptor 10 to 40 wt%, and lactoferrin 20 to 50 wt%.

In the cosmetic composition for moisturizing according to an embodiment of the present invention, the immunoglobulin may include any one or more selected from the group consisting of IgG, SIgA and IgM.

In the cosmetic composition for moisturizing according to an embodiment of the present invention, the immunoglobulin may include 1,000 to 6,000 g / ml of IgG, 500 to 1,500 g / ml of SIgA and 500 to 1,500 g / ml of IgM.

In the cosmetic composition for moisturizing according to an embodiment of the present invention, the protein mixture may be any one selected from among insulin-like growth factor (IGF-1), vascular endothelial growth factor (VEGF) Or more of the above growth factors.

In the cosmetic composition for moisturizing according to an embodiment of the present invention, the spirulina extract may contain 10 to 200 parts by weight of the solid content contained therein, based on 1 part by weight of the protein mixture.

In the cosmetic composition for moisturizing according to an embodiment of the present invention, the spirulina extract may be obtained by mixing the spirulina powder with a solvent, heating and extracting the mixture, followed by filtration and concentration under reduced pressure.

In the cosmetic composition for moisturizing according to an embodiment of the present invention, the spirulina may be at least one selected from the group consisting of spirulina apatensis and spirulina maxima.

The moisturizing cosmetic composition according to an embodiment of the present invention may further include at least one selected from the group consisting of ascorbic acid, kojic acid, arbutin, niacinamide, and N-acetylglucosamine.

The moisturizing cosmetic composition according to one embodiment of the present invention can be prepared into any one form selected from the group consisting of lotion, lotion, cream, essence, ointment, cleanser, spray, gel and patch.

The cosmetic composition for moisturizing according to an embodiment of the present invention may be one for improving atopic skin.

The cosmetic composition for moisturizing according to the present invention has the effect of providing long-lasting moisturizing performance without irritation to the skin. In addition, since the user does not feel a sense of shearing from the skin, it has an excellent feeling of use.

In addition to the moisturizing function, the cosmetic composition for moisturizing the present invention can be used in areas having skin troubles or areas with skin diseases. In addition to the healing of environmental skin diseases such as atopic dermatitis and skin allergy, It has an advantageous effect on skin improvement such as strengthening or skin regeneration.

Hereinafter, the cosmetic composition for moisturizing the present invention will be described in detail with reference to the accompanying drawings. The invention can be better understood by the following examples. The following examples are for illustrative purposes only and are not intended to limit the scope of protection defined by the appended claims. The technical terms and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention belongs, unless otherwise defined.

The inventors of the present invention have been studying new materials for a cosmetic composition for protecting the skin by providing a cosmetic composition comprising a combination of components of a specific protein containing immunoglobulin, epidermal growth factor receptor and lactoferrin and Spirulina extract It has been confirmed that it is not only irritating to the skin but also feels no sense of heterogeneity from the skin as well as being able to impart sustained moisturization to the skin for a long time. Furthermore, the cosmetic composition to be provided in the present invention is effective for improving long-term moisturizing performance, as well as for healing environmental skin diseases such as atopic dermatitis, enhancing skin barrier function and improving skin such as anti- Thereby completing the present invention.

One aspect of the present invention relates to a moisturizing cosmetic composition comprising a protein mixture containing an immunoglobulin, an epidermal growth factor receptor (EGFR) and a lactoferrin, and a Spirulina extract.

The moisturizing cosmetic composition according to the present invention not only keeps the moisturizing effect on the skin for a long period of time by including Spirulina extract at the same time as the specific protein components but also is effective for skin sedation and healing against environmental skin diseases such as atopic dermatitis , And has a property to enhance the skin barrier function or to improve the skin.

In the present invention, the protein mixture is characterized by containing an immunoglobulin, epidermal growth factor receptor and lactoferrin. The combination of the above protein components is advantageous not only in improving the moisturizing performance but also in calming and healing the skin diseases and improving the skin. Compared to the low feeling due to the heterogeneity of the conventional moisturizer, the skin component does not irritate the skin, This excellent performance can be achieved.

Specifically, among the protein mixtures of the present invention, the immunoglobulin enhances the moisturizing function of the skin itself as well as the skin moisturizing function. That is, it plays a more effective role in maintaining long-term moisturizing performance of the skin. It has a characteristic of exhibiting a long-term moisturizing performance and a remarkable synergistic effect of skin cell activity in combination with a epidermal growth factor receptor that helps skin cell activity in a protein mixture. However, when the immunoglobulin is not contained in the protein mixture, it is difficult to maintain the long-term moisturizing performance and the function of the epidermal growth factor receptor can not be expected to be improved.

The immunoglobulin is characterized in that it comprises any one or more selected from the group consisting of IgG, SIgA and IgM. In particular, the combination of epidermal growth factor receptor and lactoferrin is effective in preventing skin diseases or accelerating the recovery of damaged skin, and can be used for sedating and healing skin diseases, skin regeneration It is possible to realize a remarkable effect on skin improvement.

In the present invention, IgG (Immunoglobulin G) as an immunoglobulin is capable of neutralizing toxic substances or protecting skin damage from bacteria, viruses, fungi, etc., and is effective in calming skin diseases and is advantageous for skin improvement. In addition, it has cell affinity and improves the feeling of use without irritation to the skin as a moisturizing agent, and is effective for long-term skin moisturizing performance.

The secretory immunoglobulin A (sIgA) is a secretory immunoglobulin A that protects against infections such as bacteria and viruses and significantly reduces skin damage or skin irritation due to external environmental changes such as temperature and humidity.

The IgM (Immunoglobulin M) can exterminate bacteria that penetrate the skin, and can further improve the performance of IgG and SIgA in the immunoglobulin. At the same time, it is advantageous in inducing aggregation of protein components to realize the desired effect in the present invention in combination with other components in the composition.

The combination of these immunoglobulin components not only achieves long-lasting skin moisturizing effect, but also enhances skin barrier by promoting activity of skin autologous cells in combination with epidermal growth factor receptor and lactoferrin in protein mixture, However, when any one of these ingredients is not included, it is difficult to expect an improvement in moisturizing performance and a skin improvement effect is insignificant.

The immunoglobulin in the protein mixture is not particularly limited in the composition ratio of the above components. However, in order to realize the above effects in combination with other components in the protein mixture, it is preferable that the IgG is 1,000 to 6,000 占 퐂 / ml, SIgA is 500 to 1,500 Ml and IgM 500 to 1,500 占 퐂 / ml, more preferably 2,000 to 4,000 占 퐂 / ml, SIgA 800 to 1,200 占 퐂 / ml and IgM 800 to 1,200 占 퐂 / ml. When the above-mentioned range is satisfied, it is possible to remarkably reduce irritation caused by skin troubles, to improve skin moisturization and skin protection as well as to promote skin autocellular activity, thereby improving the skin, but this is a non-limiting example and is not limited to the above- .

In the present invention, the epidermal growth factor receptor (EGFR) of the protein mixture promotes the production of new cells and can stimulate wound healing and tissue regeneration. The skin is aged, the cell regeneration ability is delayed, the skin layer is thickened, and the self-moisturizing performance deteriorates. The epidermal growth factor receptor enhances the function of the skin including the skin self-moisturizing performance by the combination of immunoglobulin and lactoferrin in the protein mixture And to stimulate new growth of skin cells. When the epidermal growth factor receptor is not contained in the protein mixture, the moisturizing performance may be deteriorated, and the skin improvement according to the combination with other ingredients can not be expected, in particular, the sedative and healing effect of environmental skin diseases such as atopic dermatitis.

In the present invention, the lactoferrin in the protein mixture can achieve anti-allergy and anti-inflammatory properties of the skin. The lactoferrin promotes the production of secreted substances in the skin itself and promotes the recovery of the skin tissue, thereby helping to cure skin diseases. In particular, the combination of the lactoferrin with other ingredients in the protein mixture strengthens the skin barrier function, And has a characteristic capable of realizing a remarkable synergistic effect of performance. If lactoferrin is not contained in the protein, sedation against skin diseases such as atopic dermatitis can not be expected and the healing effect is insignificant. Also, the effect of improving the moisturizing performance can not be seen.

In the present invention, the protein mixture may be used as an object to be extracted without any limitations as long as it contains a combination of specific components of the immunoglobulin, epidermal growth factor receptor and lactoferrin. That is, those obtained by separating and purifying a substance containing the specific components can be used. The protein mixture containing the combination of the above specific components may preferably be obtained from colostrum but is not necessarily limited thereto. More preferably, those obtained from cow's colostrum within about 3 days immediately after birth can be used.

Specifically, the protein mixture comprises 20 to 50% by weight of immunoglobulin, 10 to 40% by weight of epidermal growth factor receptor and 20 to 50% by weight of lactoferrin, preferably 25 to 45% by weight of immunoglobulin, 15 to 35% By weight and lactoferrin 25 to 45% by weight. When the above range is satisfied, the skin moisturizing performance can be enhanced, and it is more effective in terms of long-lasting moisturizing performance, skin disease healing and skin improvement.

In addition, the protein mixture of the present invention may further comprise one or more growth factors selected from among insulin-like growth factor (IGF-1), vascular endothelial growth factor (VEGF), and metastatic growth factor (TGF-β1).

Insulin-like Growth Factor-1 (IGF-1) stimulates cell growth and proliferation, and particularly helps to regenerate damaged cells. This can be combined with other ingredients in the protein mixture to achieve the effect of promoting skin regeneration damaged by skin diseases.

The Vascular Endothelial Growth Factor (VEGF) promotes the collagen synthesis of fibroblasts and induces the secretion of new blood vessels or other regeneration promoting factors in skin damaged areas. In particular, And has the property of increasing the melanin pigment, it is more effective in healing atopic dermatitis in combination with other ingredients in the protein mixture.

The transgional growth factor (TGF-β1, Transforming Growth Factor-β1) is a growth factor that regulates physiological activity by promoting collagen biosynthesis, preventing cell deposition and promoting cell proliferation. Regeneration and recovery can be promoted. Thus, by combining with other components in the protein mixture, it is possible to realize a more advantageous effect for enhancing the moisturizing performance and for curing skin diseases.

That is, the protein mixture containing these components can provide long-term continuous moisturizing performance in combination with other ingredients in the moisturizing cosmetic composition, prevent or cure environmental skin diseases such as atopic dermatitis, Restoration, induction of epithelial cell growth, and is more effective for skin improvement. At this time, the above-mentioned components can be used by adjusting the content within a range not to impair the effect of the present invention.

In the present invention, by including a combination of Spirulina extract together with the above-mentioned specific protein mixture, it is possible to overcome the limitations of the conventional humectant, that is, it can not maintain a long-lasting moisturizing performance or gives a skin sensation to the skin, Which not only greatly improves the skin moisturizing performance but also has a characteristic of being more advantageous for maintaining healthy skin through sedation and healing of skin diseases such as atopic dermatitis and skin improvement. In addition, it is possible to effectively inhibit skin damage due to other ingredients used in the formulation of the cosmetic composition according to the present invention, thereby achieving a remarkable synergistic effect depending on the combination with the protein mixture. However, The above effect can not be expected.

In the present invention, the spirulina extract can be obtained by pulverizing the spirulina harvested and extracting it by a conventional method. The spirulina is a blue-green cyanobacterium having a high protein content and is advantageous in terms of skin improvement in addition to the desired skin moisturizing effect in the present invention. In addition, the above-mentioned spirulina is chelated with an amino acid and thus has a high absorption rate, is excellent in compatibility with the protein mixture, and is advantageous in improving the performance of components in the composition.

The spirulina may be any one selected from the group consisting of spirulina aplatensis and spirulina maxima, though it is not limited to its kind.

The spirulina extract may be an extract obtained by mixing spirulina with a solvent, heating and extracting the mixture, followed by filtration and concentration under reduced pressure. The Spirulina extract may be in the form of a solid containing liquid. At this time, the Spirulina extract may contain 1 to 20% by weight, preferably 5 to 15% by weight, of the solid content contained in the liquid phase. The spirulina extract may be filtered and concentrated under reduced pressure, followed by freeze-drying to obtain a powder.

The Spirulina extract is a solvent for obtaining an extract, and may be an alcohol having 1 to 4 carbon atoms such as purified water, methanol, ethanol, propanol, isopropanol, butanol, etc., acetone acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane, and cyclohexane. The solvent is preferably selected from the group consisting of acetone, ether, benzene, chloroform, But are not limited thereto. Ethanol may be preferably used.

The extraction method for obtaining the spirulina extract is not limited to a specific one. However, the extraction method for obtaining the spirulina extract is not limited to a specific one, but may be any one or a combination of two or more selected from a hot water extraction method, a cold extraction extraction method, a reflux cooling extraction method, a solvent extraction method, a steam distillation method, an ultrasonic extraction method, Can be used.

In one embodiment, a mixture obtained by mixing the dried spirulina powder with ethanol at a weight ratio of 1: 5 to 1:20 can be obtained from a step of heat extraction at 50 to 70 캜, preferably 55 to 65 캜, for 6 to 12 hours have. At this time, it is preferable that the mixture of the dried spirulina powder and ethanol is subjected to ultrasonic treatment so that uniform mixing can be performed. It is also more effective to perform ultrasonic treatment after extraction.

The Spirulina extract is prepared by aging the extract obtained at the extraction step at 5 to 20 ° C for 1 to 7 days in order to improve the compatibility with the protein mixture in the composition and to maintain the long-term moisturizing performance It has advantageous characteristics.

The spirulina extract may contain 10 to 200 parts by weight, preferably 50 to 100 parts by weight, of the solid content contained in the extract, based on 1 part by weight of the protein mixture. When the above range is satisfied, not only maximization of the moisturizing performance can be realized but also long-term continuous moisturizing performance can be realized due to the synergistic effect according to the combination with the protein mixture, and furthermore, the skin improving effect can be greatly improved. In addition, the cosmetic composition of the present invention has advantageous properties in terms of ensuring the quality stability of the product manufactured using the cosmetic composition of the present invention, but is not limited to the above-described numerical range.

The cosmetic composition for moisturizing according to the present invention may further comprise at least one selected from the group consisting of ascorbic acid, kojic acid, arbutin, niacinamide and N-acetylglucosamine. They are advantageous in terms of moisturizing and improving skin by combination with the above-mentioned protein mixture and Spirulina extract, and are more effective in effectively protecting the skin. At this time, although the content of the components is not limited to a great extent in the cosmetic composition, it is preferable that the composition contains 1 to 20% by weight, preferably 5 to 15% by weight of the total composition, And is not limited to the above numerical range.

The cosmetic composition for moisturizing according to the present invention may further contain at least one selected from the group consisting of ethylene glycol, diethylene glycol, triethylene glycol, butylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol , 1,3-butylene glycol, glycerin, diglycerin, propanediol, 1,2-hexanediol, sodium hyaluronate, and trehalose. But is not limited to.

The moisturizing cosmetic composition according to the present invention may further comprise an active ingredient exhibiting the same or similar function. For example, it can include known skin elasticity improvement, pore shrinkage, antibacterial, acne suppression, sebum control, skin barrier improvement, skin immunity normalization, anti-inflammation, trouble improvement, skin moisturizing and skin texture improving ingredients. This can further enhance the synergistic effect of skin improvement in combination with the above-described composition for moisturizing cosmetic composition according to the present invention.

In addition, the cosmetic composition for moisturizing according to the present invention may further contain ingredients commonly added to cosmetic compositions in addition to the above-mentioned ingredients, such as oil, purified water, surfactant, thickener, antioxidant, pH adjusting agent, neutralizing agent, solubilizing agent, defoamer, Chelating agents, coloring agents, preservatives, and flavoring agents. These contents can be appropriately adjusted as needed.

Examples of the surfactant include polyoxyethylene sorbitan monolaurate, polyethylene glycol, and phage-hydrogencarbonate castor oil, which are added to improve the dispersibility of the cosmetic composition, and preferably polyoxyethylene Tween 20, a sorbitan monolaurate, and a fat-60 hydrogenated castor oil, which is a lipophilic surfactant, may be used in combination, but the present invention is not limited thereto.

The thickener may be at least one selected from the group consisting of carbomer, acrylate, xanthan gum and cellulose gum. In addition, a powder of a known inorganic substance or grain, such as yellow ocher, bentonite, tourmaline, elvan, calcium carbonate, , Wheat, wheat, buckwheat, brown rice, soybean, red bean, mung bean, and corn powder may be used, but the present invention is not limited thereto.

Examples of the antioxidant include benzoic acid, paraoxybenzoic acid ester, imidazolidinyl urea, a mixture of methylchloroisothiazolinone, phenoxyethanol, DMDM hydantoin, methyl paraben, ethyl Parabens, and propylparaben. However, the present invention is not limited thereto.

Examples of the pH regulator include organic acids such as citric acid, succinic acid, tartaric acid, lactic acid, malic acid, fumaric acid, maleic acid and glycolic acid, inorganic acids such as hydrochloric acid, sulfuric acid, phosphoric acid and nitric acid, sodium salts, potassium salts or ammonium salts of these acids, Or a mixture of two or more members selected from the group consisting of anionic and nonionic surfactants.

The cosmetic composition according to the present invention may be applied to cosmetics such as lotion such as soft lotion, facial lotion, body lotion and the like, nutrition cream, moisture cream, eye cream, essence, ointment, , A gel, a patch, and a pack.

The cosmetic composition according to the present invention has an excellent skin soothing effect, healing and improving effect on atopic dermatitis, in addition to improvement in moisturizing performance owing to interactions between ingredients contained therein. That is, the cosmetic composition for moisturizing according to the present invention may be one for improving atopic skin. This is particularly effective in preventing, ameliorating, and treating infants' atopy. Further, the present invention can provide a cosmetic for improving atopic skin comprising the cosmetic composition for improving atopic skin.

Hereinafter, the present invention will be described in detail with reference to examples. However, these are for the purpose of illustrating the present invention in more detail, and the scope of the present invention is not limited by the following examples.

(Example 1) Preparation of protein mixture

1,000 g of colostrum, which is obtained by recovering a liquid from a mammary gland within 24 hours of birth, containing immunoglobulin (IgA), epidermal growth factor receptor (EGFR) and lactoferrin, is degreased followed by delipidization and then selectively separating the proteins. From the protein, a protein mixture was obtained by mixing 40% by weight of immunoglobulin, 20% by weight of epidermal growth factor receptor and 40% by weight of lactoferrin. At this time, the immunoglobulin used was one containing 3,520 占 퐂 / ml of IgG, 1,050 占 퐂 / ml of SIgA and 980 占 퐂 / ml of IgM.

(Example 2) Preparation of protein mixture

In Example 1, a protein mixture was obtained in the same manner as in Example 1, except that 15% by weight of immunoglobulin, 45% by weight of epidermal growth factor receptor (EGFR) and 40% by weight of lactoferrin were mixed.

(Example 3) Preparation of protein mixture

In Example 1, a protein mixture was obtained in the same manner as in Example 1, except that 50% by weight of immunoglobulin, 5% by weight of epidermal growth factor receptor (EGFR) and 45% by weight of lactoferrin were mixed.

(Comparative Example 1) Preparation of protein mixture

In Example 1, a protein mixture was obtained in the same manner as in Example 1, except that 50% by weight of immunoglobulin and 50% by weight of lactoferrin were mixed.

(Comparative Example 2) Preparation of protein mixture

In Example 1, a protein mixture was obtained in the same manner as in Example 1, except that 50% by weight of the epidermal growth factor receptor and 50% by weight of lactoferrin were mixed.

(Example 4) Preparation of Spirulina extract

First, a mixture of 100 g of spirulina platensis in the form of dried powder in a weight ratio of 1: 5 with ethanol was extracted with ultrasonic at 100 kHz at a temperature of 80 캜 for 6 hours to obtain an extract. The obtained spirulina extract was filtered and concentrated under reduced pressure, and then lyophilized to obtain a powdery spirulina extract.

(Example 5)

100 g of a cream-form cosmetic composition was prepared using 10 g of the mixture prepared by mixing the protein mixture prepared in Example 1 and the powdered Spirulina extract obtained in Example 4 in a weight ratio of 1:50. To the cream composition, 7 g of cellulose gum, 12 g of glycerin, 10 g of butylene glycol, 4 g of polysorbate 60, 5 g of sorbitan sesqui-chelate, 16 g of betaine, 4 g of carbomer, 2 g of propylparaben, . Moisturizing and atopic skin tests were conducted on the cream formulations of the cosmetic compositions prepared above.

(Example 6)

Example 5 was carried out in the same manner as in Example 5 except that the protein mixture prepared in Example 2 was used instead of the protein mixture prepared in Example 1. [

(Example 7)

Example 5 was carried out in the same manner as in Example 5, except that the protein mixture prepared in Example 3 was used instead of the protein mixture prepared in Example 1. [

(Comparative Example 3)

Example 5 was carried out in the same manner as in Example 5, except that the protein mixture prepared in Example 2 was used instead of the protein mixture prepared in Comparative Example 1. [

(Comparative Example 4)

Example 5 was carried out in the same manner as in Example 5 except that the protein mixture prepared in Example 2 was used instead of the protein mixture prepared in Comparative Example 2. [

(evaluation)

Experiments were conducted on long-term moisturizing effect of the cream formulations prepared in Examples and Comparative Examples. After applying the cream to the face for 10 minutes, the cream was cleaned and then the moisturizing effect was measured using Corneometer CM820PC (Coourage + Khazaka, Germany) and Tewameter TM 210 (Coourage + Khazaka, Germany). Five healthy women (mean age 35 years) were measured and their mean values were measured. The instrument used the Corneometer to quantify the moisture content of the skin from 0 to 150 with changes in the electrical conductivity according to the skin moisture content, and measured the TEWL, ie, the degree of water loss on the epidermis, using a Tewameter. The test was carried out for one week, and after three days and seven days, it was measured using a device, and the results are shown in Table 1 below.

division term Example 5 Example 6 Example 7 Comparative Example 3 Comparative Example 4 Electrical conductivity 3 days 80 74 65 49 43 7 days 72 63 51 23 18 Water loss amount
(g / m < 2 > / h) 3 days 19 22 26 48 51 7 days 31 37 41 63 89

As shown in Table 1, the cream according to Example 5 including the cosmetic composition for humidification of Example 1 according to the present invention was the most excellent in moisturizing performance. On the other hand, in Comparative Examples 3 and 4, the moisturizing effect was remarkably lowered, and the water loss rapidly decreased.

In addition, the skin elasticity performance test was conducted as an experiment on the skin improvement effect one week after using the cream prepared in Examples and Comparative Examples. The test conditions were 30 healthy women (mean age 35 years) in a constant temperature and humidity room at 22 ℃ and 45% relative humidity. The measuring device was a skin elasticity tester (Cutometer SEM 575, C + KElectronic Co., Germany). In addition, sensation of use was sensually evaluated. The evaluation items were the skin texture, satisfaction with the moisturizing effect, and the degree of skin irritation, respectively, and the average value was described as 5 points satisfaction.

division Example 5 Example 6 Example 7 Comparative Example 3 Comparative Example 4 Skin elasticity 56.4 42.3 41.5 30.2 26.7 Feeling sensory score 4.9 4.4 4.3 3.1 3.0

In addition, experiments on the improvement effect on atopic dermatitis were conducted. 15 patients with atopic diseases from 5 to 20 years of age were treated. After applying the solution to the affected area every day, the degree of itching and the degree of improvement of atopic dermatitis were judged by the examination and visual examination, and sensory evaluation was made. The results were as follows: 1) no itching was observed and 2) atopic dermatitis was significantly improved. As a result, in Example 5 according to the present invention, the skin elasticity and feeling of use as well as the efficacy against atopic dermatitis were extremely excellent. On the other hand, Comparative Examples 3 and 4 showed significantly lower scores than the Examples.

division Example 5 Example 6 Example 7 Comparative Example 3 Comparative Example 4 Itching 4.8 4.4 4.3 2.7 2.4 Atopic dermatitis improvement 4.7 4.3 4.0 2.3 2.1

While the invention has been shown and described with reference to certain preferred embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the above description does not define the scope of the present invention, which is defined by the limitations of the following claims.

Claims (11)

A moisturizing cosmetic composition comprising a protein mixture containing an immunoglobulin, a epidermal growth factor receptor (EGFR) and a lactoferrin, and a Spirulina extract. The method according to claim 1,
Wherein the protein mixture comprises 20 to 50% by weight of immunoglobulin, 10 to 40% by weight of epidermal growth factor receptor, and 20 to 50% by weight of lactoferrin. The method according to claim 1,
Wherein the immunoglobulin comprises any one or more selected from the group consisting of IgG, SIgA, and IgM. The method of claim 3,
Wherein the immunoglobulin comprises IIgG at 1,000 to 6,000 占 퐂 / ml, SIgA at 500 to 1,500 占 퐂 / ml and IgM at 500 to 1,500 占 퐂 / ml. The method according to claim 1,
Wherein the protein mixture further comprises one or more growth factors selected from among insulin-like growth factor (IGF-1), vascular endothelial growth factor (VEGF), and metastatic growth factor (TGF-? 1). The method according to claim 1,
Wherein the spirulina extract comprises 10 to 200 parts by weight of the solid content contained in 1 part by weight of the protein mixture.
The method according to claim 1,
Wherein the spirulina extract is an extract obtained by mixing spirulina powder with a solvent and heating and extracting the mixture, followed by filtration and concentration under reduced pressure. The method according to claim 1,
Wherein the spirulina is at least one selected from the group consisting of spirulina platensis and spirulina maxima. The method according to claim 1,
Wherein the cosmetic composition further comprises at least one selected from the group consisting of ascorbic acid, kojic acid, arbutin, niacinamide, and N-acetylglucosamine. 9. The cosmetic composition according to any one of claims 1 to 9, which is prepared from any one of the formulations selected from the group consisting of lotion, lotion, cream, essence, ointment, cleanser, spray, gel and patch A cosmetic composition for moisturizing. 9. The cosmetic composition for improving atopic skin according to any one of claims 1 to 9.