KR20230111926A - A cosmetic composition comprising vegetable collagens using natural microneedle, and a manufacturing process thereof - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing and improving wrinkles or loss of elasticity, including natural fine needle powder and a vegetable collagen component, and a manufacturing method thereof. According to the composition of the present invention, the active ingredient can effectively penetrate into the skin to improve wrinkles or provide elasticity to the skin, thereby maintaining skin health.

Description

A cosmetic composition comprising vegetable collagens using natural microneedle, and a manufacturing process thereof}

The present invention relates to a cosmetic composition for preventing and improving wrinkles or elasticity reduction, including natural fine needles and vegetable collagen, and a method for manufacturing the same, wherein active ingredients including vegetable collagen effectively penetrate into the skin to improve wrinkles or provide elasticity to the skin, thereby maintaining skin health.

Due to the deterioration of environmental factors such as fine dust and abnormal high temperatures, the skin is more damaged than before, and skin diseases are increasing, as well as increasing income levels and entering an aging society, increasing interest in improving skin aging. Therefore, a lot of research related to skin diseases and aging has been made, and the function of collagen for the skin has been revealed. Among them, when collagen metabolism is activated by promoting collagen synthesis, it has been found that the dermis component is increased to exhibit effects such as wrinkle improvement, elasticity enhancement, skin strengthening and wound healing, and accordingly, attention has been paid to the effective delivery of collagen to the skin. In accordance with such technical needs, many products in which vegetable collagen such as chlorella extract as well as animal collagen have been applied to skin-strengthening cosmetic compositions have been released, but they have not received much attention among consumers. In addition, many products prepared as a cosmetic composition for skin protection by directly blending collagen have been released.

On the other hand, Geumhwagyu (also referred to as Hibiseu Smanihot L., or Hibiscus Manihot), which is similar to vegetable collagen, which is a component of the present invention, is known to have an excellent effect of promoting collagen production in skin fibroblasts, and a number of compositions using it are disclosed. For example, Patent Document 1 discloses a cosmetic composition for improving skin wrinkles containing an extract of Geumhwagyu. On the other hand, Patent Document 2 discloses a cosmetic composition for wound healing containing an extract of Geumhwagyu. In addition, Patent Document 3 discloses a cosmetic composition containing a goldenrod extract and a hibiscus fruit extract.

However, cosmetics applied to the skin surface are difficult to fully expect effects such as wrinkle improvement, elasticity enhancement, skin strengthening and wound healing as well as moisturizing action of the skin due to the difficulty of transdermal absorption of collagen, which is a polymer.

Patent Document 1: Patent Application Publication No. 10-2021-0080180 Patent Document 2: Patent Application Publication No. 10-2021-0033422 Patent Document 3: Patent Application Publication No. 10-2020-0090629

As described above, the present invention overcomes the limitations of the prior art that collagen is difficult to absorb percutaneously when applied to the skin, and it is an object of the present invention to provide a cosmetic composition for preventing and improving wrinkles or elasticity that can effectively deliver collagen to the dermis by using natural fine needles as an active ingredient.

Furthermore, it is an object to provide a method for preparing the cosmetic composition.

To achieve the above object, the present invention discloses the following.

In one embodiment of the present invention, the present invention relates to a cosmetic composition for preventing and improving wrinkles or loss of elasticity comprising natural fine needle powder and a vegetable collagen component.

Further, in another embodiment of the present invention, the present invention relates to a cosmetic composition further comprising a fermented product of bifida in the cosmetic composition.

Further, in another embodiment of the present invention, the present invention relates to a cosmetic composition further comprising a plant extract or vegetable oil in the cosmetic composition.

Furthermore, in another embodiment of the present invention, the present invention comprises the steps of obtaining a natural fine needle raw material powder; and a method for producing a cosmetic composition comprising mixing the obtained natural fine needle raw material powder with a composition containing vegetable collagen powder to prepare the cosmetic composition as described above.

According to the cosmetic composition of the present invention, by effectively delivering an active ingredient derived from vegetable collagen as an active ingredient to the dermis using natural microneedles, it is possible to prevent and improve wrinkles or elasticity loss by strengthening moisturizing and anti-aging effects in the skin.

Figure 1a shows an optical micrograph (x50 magnification) of natural microneedles among the components of the present invention.
Figure 1b shows an optical micrograph (x200 magnification) of natural microneedles among the components of the present invention.
Figure 1c shows an optical micrograph (x400 magnification) of natural microneedles among the components of the present invention.
FIG. 1D is an image of hydrolyzed sponge powder derived from Spongilla lacustris taken with a scanning microscope, and B is an illustration of various bone fragment structures seen in micro-needles present in hydrolyzed sponge.
2 illustrates and shows microneedles derived from a conventional injection needle delivered to various parts of the skin and the natural microneedle of the present invention selectively delivered to the dermis.
Figure 3 shows the manufacturing process of the natural microneedle component of the present invention.
4a to 4c are photographs showing the process of a human body patch test (closed patchtest) using a fin chamber as performed as part of a skin irritation test with respect to the composition of the present invention.
5 is a graph showing the results of a transdermal infusion test showing the degree to which the natural microneedle-containing composition of the present invention and a control deliver caffeine as a test ingredient as a delivery material over time.
Figure 6a shows contrasting frontal photographs of a male subject in his 40s before and after 4 weeks of use of the composition of the present invention.
Figure 6b shows a contrasting side view of a female subject in her 60s before and after 4 weeks of use of the composition of the present invention.
Figure 6c shows a comparison of frontal photographs of a male subject in his 70s before and after 4 weeks of use of the composition of the present invention.

The cause of wrinkles is a natural aging phenomenon that occurs with age. The most common reason is the degeneration of the dermis due to continuous exposure to ultraviolet rays, which causes the skin to dry out and wrinkles. The main causes of wrinkles are a decrease in elasticity due to aging, a decrease in facial volume, dry skin, and movement of facial expression muscles. Wrinkles occur on the human body, especially on the face, due to genetic predisposition or acquired environmental influences, and lifestyle habits such as eating stimulating favorite foods such as cigarettes and alcohol can also affect wrinkle formation.

In general, collagen, as a major component of the cell matrix, plays an important role in maintaining the tension and flexibility of the skin due to its high strength in binding to moisture as well as the role of structure as an extracellular matrix in the epidermal layer, and it is known to be effective in reducing fine wrinkles appearing on the skin. Therefore, various methods for alleviating skin wrinkle formation by replenishing collagen to skin tissue have been disclosed. Among them, a method of morphologically removing deep wrinkles formed in the dermis by lifting the skin by subcutaneous injection of collagen is generally widely used. However, the injected collagen is quickly absorbed and does not fundamentally affect the wrinkles of the epidermis.

As a method of alleviating wrinkles in the epidermal layer, a method of directly applying collagen to the skin has been introduced, but collagen molecules having a high molecular weight are difficult to penetrate the surface of the stratum corneum. Nevertheless, various methods have been devised to effectively penetrate collagen into the stratum corneum and the granular layer, but the effect is still unsatisfactory enough to leave much room for improvement.

Therefore, according to one aspect of the present invention, the composition of the present invention aims to prevent and improve wrinkles or loss of elasticity by effectively delivering active ingredients such as collagen and protein by physically penetrating the bone fragments under the skin.

One aspect of the present invention relates to a cosmetic composition for preventing and improving wrinkles or elasticity loss comprising natural fine needles and vegetable collagen. In the present invention, 'natural fine needle' can be obtained from the powder of hydrolyzed sponges. Figure 1d A shows an image taken with a scanning microscope of hydrolyzed sponge powder derived from Spongilla lacustris, which is a kind of natural microneedles among the components of the present invention, and Figure 1d B shows various bone fragment structures found in microneedles present in hydrolyzed sponges. That is, the natural microneedle of the present invention acts as a fine needle as it is expressed, and can deliver active ingredients transdermally more accurately and conveniently than when using a conventional general injection needle (see FIG. 2).

The natural microneedle raw material of the present invention includes natural spongy bone fragments and synthetic spongy bone fragments, but is preferably natural spongy bone fragments, particularly Spongilla lacustris L. Spongilla fragilis Leidy , and/or Ephydtia fluviatilis bone fragments from freshwater sponges. Natural fine needles are preferably included in an amount of 0.05% by weight or more and 20% by weight or less, preferably 0.1% by weight or more and 15% by weight or less, more preferably 0.5% by weight or more and 12% by weight or less, particularly preferably 1% by weight or more and 7% by weight or less, most preferably 3% by weight or more and 5% by weight or less, based on the total weight of the composition. If the content of natural microneedles is less than 0.05% by weight relative to the total weight of the composition, transdermal penetration is significantly reduced, and if it exceeds 20% by weight, miscibility with other components in the composition is poor and transdermal delivery is no longer increased.

In addition, the natural fine needles preferably have a particle size of 0.2 mm or less, preferably 0.1 mm or less, more preferably 0.05 mm or less, and most preferably 0.01 mm or less in the composition of the present invention. In addition, it is preferable that the particle size is 0.001 mm or more, preferably 0.005 mm or more. If the particle size exceeds 0.2 mm, the heterogeneity in the composition is excessively increased, and if the particle size is less than 0.005 mm, adsorption between the natural fine needle particles and the active ingredient is poor, resulting in poor transferability of the active ingredient.

In the present invention, 'vegetable collagen' refers to a component similar to animal collagen as containing glycine, proline, and hydroxyproline, and means mainly extracted from mulberry extract (hibiscus). Plant collagen is preferably included in an amount of 0.05% by weight or more and 20% by weight or less, preferably 0.1% by weight or more and 15% by weight or less, more preferably 0.5% by weight or more and 12% by weight or less, particularly preferably 1% by weight or more and 7% by weight or less, most preferably 3% by weight or more and 5% by weight or less, based on the total weight of the composition. If the content of natural microneedles is less than 0.05% by weight of the total composition, the effect of supplying the active ingredient derived from plant collagen is reduced, and if it exceeds 20% by weight, miscibility with other ingredients in the composition is reduced and the transdermal delivery ability is no longer increased.

In another embodiment of the present invention, the cosmetic composition further includes a fermented product of bifida. Here, 'bifida fermented product' is a filtered product of fermented bifidus lactic acid bacteria, which protects the skin from external harmful substances and increases moisturizing power. The fermented bifida used in the present invention includes, more specifically, fermented products of Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium animalis ssp, and Bifidobacterium lactis, and Bifidobacterium longum is preferred in that it is particularly helpful in strengthening the immune system. The fermented bifida product is preferably included in an amount of 0.05% by weight or more and 40% by weight or less, preferably 0.1% by weight or more and 20% by weight or less, most preferably 1% by weight or more and 10% by weight or less, based on the total weight of the composition. If the content of fermented bifida is less than 0.05% by weight relative to the total weight of the composition, the effect expected from the fermented bifida product is reduced, and if it is more than 40% by weight, miscibility with other ingredients in the composition is reduced and the transdermal delivery ability is no longer increased.

In addition, in another embodiment of the present invention, a plant extract or vegetable oil is further included in the cosmetic composition. Here, 'plant extract' refers to a component extracted from a plant and generally refers to a cosmetic composition and a component used in a cosmetic composition, preferably Tremella Fuciformis extract, rice protein extract (Oryza Sativa seed protein extract), green tea extract (Camellia sinensis leaf extract), Centella Asiatica extract, quinoa extract (Chenopodium Quinoa extract), purslane extract (Portulaca oleracea extract) , Portulaca oleracea extract, sea grape extract (Caulerpa Lentillifera Extract), annatto extract (Helianthus Annus Seed Oil), and Houttuynia cordata Extract.

On the other hand, 'vegetable oil' is not particularly limited as long as it is commonly used in cosmetic compositions, and includes, for example, hemp seed oil [Cannabis Sativa (Hemp) seed oil], vitamin tree oil (Hippophae Rhamnoides Oil), camellia oil, rosehip oil, borage oil, St. John's wort oil, shea butter oil, apricot kernel oil, etc. Rhamnoides Oil) is preferred.

The plant extract or vegetable oil is preferably included in an amount of 0.1% by weight or more and 20% by weight or less, preferably 1% by weight or more and 15% by weight or less, most preferably 3% by weight or more and 12% by weight or less, based on the total weight of the composition. If the content of fermented bifida is less than 0.1% by weight relative to the total weight of the composition, it is difficult to expect the effect, and if it exceeds 20% by weight, the miscibility with other ingredients in the composition is poor and the transdermal delivery ability does not increase any more.

In addition, the composition of the present invention may further include a protein or peptide component, for example, copper tripeptide-1, acetyl hexapeptide-8, palmitoyl tetrapeptide-7 (Palmitoyl tetrapeptide-7) and EGF (rh-Oligopeptide-1). The protein or peptide component is preferably included in an amount of 0.1% by weight or more and 20% by weight or less, preferably 1% by weight or more and 15% by weight or less, most preferably 3% by weight or more and 12% by weight or less, based on the total weight of the composition. If the content of fermented bifida is less than 0.1% by weight relative to the total weight of the composition, the expected effect of addition is reduced, and if it exceeds 20% by weight, miscibility with other ingredients in the composition is reduced and transdermal delivery ability does not increase any more.

Furthermore, the composition of the present invention may further include hydrolyzed collagen, snail secretion filtrate, dipotassium glycyrrhizate, and polyquaternium-51 as additional active ingredients. Such additional active ingredients are preferably included in an amount of 0.1% by weight or more and 20% by weight or less, preferably 1% by weight or more and 15% by weight or less, and most preferably 3% by weight or more and 12% by weight or less, based on the total weight of the composition. If the content of fermented bifida is less than 0.1% by weight relative to the total weight of the composition, the expected effect of addition is reduced, and if it exceeds 20% by weight, miscibility with other ingredients in the composition is reduced and transdermal delivery ability does not increase any more.

According to one embodiment of the present invention, as components that can be added during the preparation of the cosmetic composition, it further includes a thickener, a fat component, a moisturizer, a softener, a surfactant, a pigment, an organic powder, a UV absorber, a preservative, a bactericide, an antioxidant, a pH adjuster, alcohol, a pigment, a fragrance, a plant extract, a cooling agent, an antiperspirant, and one or more components selected from purified water.

In addition, the cosmetic composition of the present invention is processed according to a method generally applied in the cosmetic field, and can be formulated into creams, gels, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, cleansing, oils, powder foundations, emulsion foundations, wax foundations, solutions, packs, massage creams, masks and sprays.

Furthermore, another aspect of the present invention relates to a method for producing the cosmetic composition of the present invention. The method includes obtaining natural fine needle raw material powder and mixing the obtained pubic bone raw material powder into a composition containing plant collagen powder. The step of obtaining the raw material powder of the natural microneedle includes: removing impurities by ultrasonication and centrifugation; Treating sponge (sometimes referred to as 'sponge') raw material with a basic agent; neutralizing with an acidic agent; Sterilizing with a sterilizing agent; powdering step; and sifting to obtain natural fine needle raw material powder. The ultrasonic and centrifugal separation steps to further increase the purity of the final product; neutralizing; And it may further include the step of washing once to three times with purified water or distilled water for each step after the sterilizing step.

The basic agent is not particularly limited as long as it is allowed as a usable component in the preparation of the cosmetic composition, and typically includes, for example, sodium hydroxide. The acidic agent is likewise not particularly limited, and typically includes, for example, acetic acid and/or ascorbine. The sterilizing agent is also not particularly limited and typically includes hydrogen peroxide and/or ethanol. Through the treatment of the basic agent and the acidic agent, the object to be treated is hydrolyzed.

Example

Hereinafter, specific examples of the present invention will be described in more detail with reference to examples to aid understanding of the present invention. However, the following examples are merely illustrative of the contents of the present invention, but the scope of the present invention is not limited to the following examples. Spring practice of the invention is provided to more completely explain the present invention to those skilled in the art.

Example 1. Preparation of hydrolyzed sponge powder

In Example 1, as shown in FIG. 3, the natural microneedle component of the present invention was prepared as sponge powder.

First, distilled water was added to 200 g of a natural sponge obtained from freshwater sponges ( Spongilla lacrutris ), and micro impurities and microorganisms attached to natural fine needles were removed by ultrasonication and centrifugation (10.000G, 30 minutes) at room temperature for 15 minutes. Sponges were obtained. The obtained sponge was then washed three times with distilled water.

A natural sponge from which these impurities have been removed. 2 . After treatment with 700 ml of 5 M sodium hydroxide (4% wt./wt.) at 37° C. for 24 hours to remove proteins and pigments, the sample was washed three times with distilled water. Through this, soluble impurities and residual sodium hydroxide solution were removed. Subsequently, 800 ml of a 1:1 mixed solution of 2% acetic acid and 2% L-ascorbic acid solution was added to further remove trace amounts of sodium hydroxide. It was then washed three times with distilled water to remove the acetic acid and L-ascorbic acid solution.

In addition, for sterilization again, it was treated with 200 ml of 35% hydrogen peroxide solution at 37° C. for 36 hours, washed with 200 ml of 70% ethanol to sterilize, and then washed with purified water three times. Thereafter, it was dried with hot air to obtain pure sponge powder from which purified water was removed.

The obtained sponge powder was then sequentially sieved through a sieve having pores of 1 mm, 0.5 mm, and 0.2 mm, respectively, to finally obtain 170 g of sponge powder having a particle size of 0.2 mm or less.

Example 2. Preparation of compositions of the present invention that supply collagen and protein to the skin

Compositions 1 to 8 (Preparation Examples 1 to 8, respectively) of the present invention for supplying collagen and protein to the skin were prepared using the natural microneedles prepared in Example 1 above. Test compositions were constructed by combining different composition ratios of the active ingredients included in the composition, and the component ratios of the collagen-impregnated compositions prepared using the hydrolyzed sponge powder and active ingredients are shown in [Table 1]. In the content of each composition, the remaining ingredients are purified water and each ingredient is based on weight percent of the total composition.

Ingredients (% by mass) manufacturing example One 2 3 4 5 6 7 8 natural fine needle 15 10 8 7 5 One 0.5 0.1 fermented bifida 40 30 20 15 10 5 One 0.1 plant collagen 20 15 10 8 5 3 One 0.1 plant extract White Thrush Mushroom Extract 15 3 5 Rice Protein Extract 15 5 5 green tea extract 3 Purslane Extract 3 centella asiatica extract 3 Quinoa Extract 5 portulaca extract 5 5 7 Sea Grape Extract 3 Annatto Extract 3 Eoseongcho Extract 3 3 Gosam Extract 3 vegetable oil hempseed oil 5 Vitamin Tree Oil 3 peptide Copper tripeptide-1 2 Acetyl Hexapeptide-8 2 Palmitoyl Tetrapeptide-7 2 EGF (rh-Oligopeptide-1) 0.001 Other Active Ingredients hydrolyzed collagen 5 snail slime extract One Dipotassium glycyrrhizate One Polyquaternium-51 One

Example 3. Skin irritation test of the composition of the present invention

Similar to the composition of Preparation Example 1 of the present invention, Essential Preparation Examples 1 to 8 for a composition containing only natural fine needle powder, vegetable collagen, and 15% fermented bifida as the main components (based on weight%) of the present invention were prepared, and skin irritation was tested through a closed patch test as follows to determine safety to the skin (see FIGS. 4A to 4C). Thirty adults were selected as subjects, and the test site was the forearm.

After making a sample for each concentration, drop 15 μl of the sample on the test patch according to the Fin chamber user guide, place it on the upper arm, and fix it with laboratory tape (250 Finn Chambers on Scanpor ^TM ). After 24 hours, the tape was removed, and after 4 hours, the reaction of the skin to the test site was determined.

The judging criteria are as follows:

- No unusual phenomena

+ Microerythema appears

++ erythema appears

+++ Erythema and pimples appear.

skin irritation - + ++ +++ stimulation Essential Manufacturing Example 1.
(15% natural fine needles, 40% fermented bifida, 20% vegetable collagen and purified water) 10 10 8 2 0.666667 Essential Manufacturing Example 2.
(10% natural fine needles, 30% fermented bifida, 15% vegetable collagen and purified water) 14 8 7 One 0.533333 Essential manufacturing example 3.
(Natural microneedle 8%, bifida fermented product 20%, vegetable collagen 10% and purified water) 22 5 2 One 0.266667 Essential Manufacturing Example 4.
(7% natural fine needles, 15% fermented bifida, 8% vegetable collagen and purified water) 22 5 2 One 0.266667 Essential manufacturing example 5.
(Natural microneedle 5%, bifida fermented product 10%, vegetable collagen 5% and purified water) 29 One 0 0 0.033333 Essential Manufacturing Example 6.
(1% natural fine needles, 5% fermented bifida, 3% vegetable collagen and purified water) 30 0 0 0 0 Essential manufacturing example 7.
(Natural microneedle 0.5% bifida fermented product 1%, vegetable collagen 1% and purified water) 30 0 0 0 0 Essential manufacturing example 8.
(Natural microneedle 0.1% bifida fermented product 0.1%, vegetable collagen 0.1% and purified water) 30 0 0 0 0

Example 4. Skin irritation test of the composition of the present invention

In order to find out the skin improvement rate of the above essential preparation examples for the composition of the present invention, a patch test on the human body was conducted again. In the Essential Preparation Example, the stimulation was tested for Essential Preparation Examples 5 to 8, which were less than or equal to 0.1 except for stimulation. Thirty adults were selected as subjects, and the test site was the face in the 4-week test.

skin wrinkle improvement rate skin condition improvement Number of people % Essential Manufacturing Example 5 very good 18 60 good night 7 23.4 is average 4 13.3 Yes One 3.3 Sum 30 100 Essential Manufacturing Example 6 very good 11 36.7 good night 9 30 is average 7 23.3 Yes 3 10 Sum 30 100 Essential Manufacturing Example 7 very good 2 6.7 good night 7 23.4 is average 16 53.2 Yes 5 16.7 Sum 30 100 Essential Manufacturing Example 8 very good One 3.4 good night 2 6.6 is average 20 66.7 Yes 7 23.3 Sum 30 100

As a result of the test evaluation, it was found that the case of Essential Preparation Example 5 was superior in skin improvement effect compared to other Essential Preparations.

Example 5. Comparative evaluation of percutaneous absorption of natural sponge-impregnated compositions

In order to reconfirm the transdermal absorption ability of the natural microneedle powder used in the composition of the present invention, a percutaneous absorption evaluation test was conducted in a test institution by referring to the Ministry of Food and Drug Safety Animal Alternative Test Guideline: Guideline for In Vitro Skin Absorption Test Guideline, OECD Guideline for Testing of Chemicals, Section 4, TG 428 "Skin absorption: in vitro Method and Caffeine assay described in USP31-NF26 (page 1590). In this experiment, nude rats Skin (nude rat skin) was used as a test material and the results were derived by HPLC analysis.

More specifically, for the convenience of tracking in Preparation Example 5 of the composition of the present invention prepared in Example 2 for nut rat skin, a composition containing caffeine ('test group') was prepared instead of the vegetable collagen component. In addition, as a control group, a composition having the same components ('control group') was used except that the main component of the composition, natural microneedles, was not included.

After applying 1 ml of each of these two types of compositions once, the accumulated amount of caffeine per area in the dermis was examined over time. 5 is a graph showing the degree to which the test group and the control group transdermally deliver caffeine as a test ingredient over time according to the experimental results.

As shown in the graph, the composition of the test group showed a much higher accumulation per area than the composition of the control group. The above graph is a graph prepared by combining the following [Table 4] (test group) and [Table 5] (control group).

test group sampling time
(hour) average emission
(μg/cm2) standard deviation of emissions
(μg/cm2) 0 0 0 0.5 13.57 13.11 One 26.06 14.75 2 57.56 15.73 4 132.34 14.71 6 224.09 21.52 8 306.11 20.3 10 393.13 25.36 12 479.98 32.72 24 867.58 41.26

control group sampling time
(hour) average emission
(μg/cm2) standard deviation of emissions
(μg/cm2) 0 0 0 0.5 3.97 3.42 One 13.17 8.78 2 38.54 19.29 4 115.13 41.55 6 206.16 57.24 8 278.2 76.65 10 348.95 91.26 12 416.84 104.58 24 614.36 156.11

More specifically, when the sampling time was 0.5 hour, the composition of the test group was 3.4 times larger than the composition of the control group, 2.0 times at 1 hour, and 1.5 times at 2 hours. The amount of accumulation per area was shown.

Since this result is a difference due to only one application, it is thought that the difference in the effect of transdermal delivery of the active ingredient can become larger over time when the composition of the present invention is repeatedly applied to the skin.

Example 6. Verification of wrinkle improvement and skin elasticity enhancement effect (1)

In order to verify the effect of improving wrinkles and enhancing skin elasticity of the composition of the present invention, pictures were taken of the facial area using a high-resolution digital camera (CANON EOS 750D) under conditions of the same brightness of indoor lighting. For standardization, photographing was performed by the same researcher, and the photographing conditions (measurement direction and location) were fixed and measured using specially designed equipment.

The human skin wrinkle improvement efficacy test was conducted on 20 female subjects aged 30 years or older, and the experimental group ('Experimental Group 2') of the composition of Preparation Example 5 of Example 2 and the control group ('Control Group 2') of the same composition as the ingredients of Experimental Group 2 except that they did not contain natural fine needles as an active ingredient were applied to the test area of the face twice a day for 8 weeks, morning and evening, respectively, before and after use of the product, respectively. (Visual evaluation of wrinkles according to Danielle's Criteria) for the degree of wrinkles was visually evaluated. Visual evaluation was conducted on the condition of wrinkles around the eyes on both sides, the condition of nasolabial folds, and facial skin elasticity, and two examiners independently evaluated the condition of the subjects. The grade of the wrinkle condition was recorded at each evaluation time point, and the average value of each tester's evaluation data was taken and analyzed.

Rating explanation 0 There are no wrinkles on the skin, and the skin texture is delicate. One Shallow wrinkles begin to appear on the skin. 2 Shallow wrinkles are visible on the skin. 3 There are many shallow wrinkles or normal wrinkles are starting to show. 4 Wrinkles are usually visible on the skin. 5 There are normal wrinkles, but deep wrinkles are not visible. 6 Usually, many wrinkles or deep wrinkles begin to appear. 7 Deep wrinkles are visible on the skin. 8 There are many deep wrinkles on the skin. 9 There are many deep wrinkles on the skin.

As a result, as shown in the evaluation results for wrinkle improvement in Table 7 below, the experimental group 2 applied with the composition of the present invention received a lower wrinkle evaluation grade over the 8 weeks of the experiment compared to the control group 2 that did not contain natural fine needles. Therefore, it was confirmed that there is an effective anti-wrinkle effect.

cosmetic composition Wrinkle grade visual evaluation (mean ± standard error) before use 4 weeks later 8 weeks later experimental group 2 5.221±0.338 5.018±0.332 4.886±0.325 Control 2 5.191±0.330 5.156±0.225 5.092±0.320

Example 7. Verification of wrinkle improvement and skin elasticity enhancement effect (2)

In order to verify the excellent transdermal penetration efficiency of the composition of the present invention, in terms of wrinkles and skin elasticity, a contrast test was performed on the difference in skin before and after 4 weeks of use (application) of the composition of Preparation Example 5 of Example 2. Figure 6a shows a frontal photograph of a male subject in his 40s before and after 4 weeks of using the composition of the present invention, Figure 6b shows a side view of a female subject in his 60s before and after 4 weeks of using the composition of the present invention, and Figure 6c shows a frontal photograph of a male subject in his 70s before and after 4 weeks of using the composition of the present invention.

From the photographs of FIGS. 6A to 6C , it can be seen that when the composition of the present invention is used, the transdermal penetration efficiency of the active ingredient is excellent, and wrinkles are noticeably improved and skin elasticity is generated after 4 weeks of use, even with just one application.

Claims (8)

A cosmetic composition for preventing and improving wrinkles or loss of elasticity, comprising a natural fine needle powder and a vegetable collagen component. The cosmetic composition according to claim 1, further comprising fermented bifida. The cosmetic composition according to claim 1, further comprising a plant extract or vegetable oil. The cosmetic composition according to claim 1, wherein the natural fine needles are included in an amount of 0.05% to 20% by weight based on the total weight of the composition, and the vegetable collagen is included in an amount of 0.05% to 20% by weight based on the total weight of the composition. The cosmetic composition according to claim 1, wherein the natural fine needles are freshwater sponges having a particle size of 0.2 mm or less. The cosmetic composition according to claim 1, further comprising at least one component selected from a thickener, a fat component, a moisturizer, a softener, a surfactant, a pigment, an organic powder, an ultraviolet absorber, a preservative, a bactericide, an antioxidant, a pH adjuster, an alcohol, a pigment, a fragrance, a plant extract, a cooling agent, an antiperspirant, and purified water. The cosmetic composition of claim 1, wherein the composition is a formulation selected from cream, gel, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing, oil, powder foundation, emulsion foundation, wax foundation, solution, pack, massage cream, mask and spray. Obtaining a natural fine needle raw material powder; and
A method for producing a cosmetic composition comprising preparing the cosmetic composition of claims 1 to 7 by mixing the natural fine needle raw material powder obtained above with a composition containing vegetable collagen powder.