KR20120107557A - Rice bran fermentation extracts with chlorophyll and the manufacturing method thereof - Google Patents

Abstract

PURPOSE: A fermented rice bran extract containing chlorophyll, and a producing method thereof are provided to use the rice bran extract as a bio-active substance or other additives. CONSTITUTION: A producing method of a fermented rice bran extract containing chlorophyll comprises the following steps: freeze-drying the chlorophyll before crushing into 70-200 meshes, and adding rice bran and distilled water to the chlorophyll to obtain a rice bran mixture having 9-15% of water(S10); firstly fermenting the mixture using enzymes and fermentation microorganisms in the rice bran and the chlorophyll(S20); adding a cocoa husk and candy cane mixture into the firstly fermented material for secondly fermenting(S30); sterilizing and drying the secondly fermented mixture, and adding a natural nutrient, medical herbs, and other ingredients to obtain a mixed medium(S40); injecting other fermentation microorganisms into the mixed medium for thirdly fermenting(S50); and sterilizing and drying the thirdly fermented material, and mixing the material with an alcohol aqueous solution for mixing, stirring and extracting. [Reference numerals] (S10) Mixing chlorophyll, rice bran, and distilled water to obtain a rice bran mixture; (S20) Firstly fermenting the rice bran mixture; (S30) Secondly fermenting the rice bran mixture; (S40) Mixing the secondly fermented mixture with natural nutrient, medical herbs, minerals, algae, grains, beans, additives, and vegetable fat skimmed powder to obtain a mixed medium; (S50) Injecting fermentation microorganisms into the mixed medium, and thirdly fermenting; (S60) Mixing and stirring the thirdly fermented mixture with an alcohol aqueous solution for extracting; (S70) And aging the obtained extract at 5-10 deg. C for 10-100 days

Description

Fermented extract of rice bran containing chlorophyll and its manufacturing method {Rice bran fermentation extracts with chlorophyll and the manufacturing method

The present invention relates to a fermented extract of chlorophyll-containing rice bran and a method for producing the same, and more specifically, pulverized barley sprouts, wheat sprouts, oat sprouts at least one selected from dried and pulverized and distilled water 9-15% It is fermented microorganism present in rice bran and chlorophyll itself. After fermentation, the crushed cocoa shell and sugar cane by-product extracted from raw sugar are fermented secondly. Natural nutrients, nutritional substances, herbal medicines, minerals Chlorophyll, which can be used as animal feed additives, fermented medicinal herbs, raw materials of foods, and other additives by fermentation extracts prepared through the third fermentation process by adding algae, grains and legumes, additives, vegetable fats and fats, and fermentation microorganisms. It relates to a fermented extract of rice bran containing the same and a method for producing the same.

Young barley, young wheat and young oat sprouts are chlorophyll foods, also known as chlorophyll. Green plants are compounds that contain many chloroplasts, elliptical structures, in the cells of their leaves. Chlorophyll appears green because its color is green, so the leaves of plants also appear green. It is contained in the grana of chloroplasts and binds to the proteins that make up the grana. There are several known chlorophylls such as a, b, c, d, e and bacterio chlorophyll a and b. These are all classified and named by the difference in structural formula of the molecule. These chlorophylls are characterized by having one atom of magnesium (Mg) in their molecules. Chlorophyll is also characterized by being insoluble in organic solvents (liquid organic compounds such as ether, benzene and chloroform) without being dissolved in water. When it receives ultraviolet rays, it emits dark red fluorescence.

The most common chlorophylls are a and b. In most plants, a and b are present in a ratio of about 3: 1. Other chlorophylls are present in very small amounts or only in certain plants. Bacterio chlorophyll is a chlorophyll found in the cells of photosynthetic bacteria. Green plants use the sun's light to combine carbon dioxide (CO ₂ ) and water (H ₂ O) to produce carbohydrates such as glucose and starch. This process is photosynthesis, where chlorophyll plays an important role in capturing the sun's light energy and converting it into chemical energy to make carbohydrates. In other words, when chlorophyll is exposed to light, the chlorophyll molecule absorbs the light energy into an excited state.

The excited state is a phenomenon in which electrons in a molecule absorb extra energy and move to a higher energy position. When the molecule is excited, it returns to its original state and releases the energy it absorbed. As the chlorophylls of the excited molecules return to their original state, the released energies gather together to bounce a pair of electrons from a particular chlorophyll molecule. The pair of electrons travel through several electron acceptors and ultimately return to chlorophyll or to a substance called NADP (a coenzyme as a phosphate compound).

The movement path of the electron is a process of transferring from a small substance having a small affinity for electrons to a large substance, in which the energy of the electron is stored in the form of chemical energy in a substance called ATP (Adenosine Triphosphate). The electrons trapped in the NADP also reduce the NADP, enabling the subsequent assimilation of carbon dioxide. In addition, when electrons released from chlorophyll are transferred to NADP, chlorophyll molecules are deficient in electrons, which are supplemented by electrons from photolysis of water molecules. Chlorophyll is the most important substance in photosynthesis because it absorbs light energy in the chloroplasts of green plants and is used to assimilate carbon dioxide into carbohydrates, an organic compound (source-chlorophyll, Naver Encyclopedia).

The effects on the body include activating cells, enhancing organ function, increasing tissue resistance, supplying chlorophyll to nerves and muscles that cannot move due to fatigue, then reactivating contraction, strengthening cardiac contraction, extending diastolic and whole heart pumps. It is said that remarkable activity of peripheral (peripheral blood vessels) expansion has been confirmed. Cardiac arrest after activity due to intoxication and fatigue has also been reported to be effective in normalizing the digestive system.

In 1935, the cell reactivation was experimentally demonstrated by tissue culture and started to be used as a therapeutic drug.

The healing promoting action promotes granulation formation and has a quick healing effect on the surface drying of the affected area and the affected area. Large-scale clinical trials on more than 1200 patients resulted in foul odor caused by contaminated anaerobic bacteria → deodorization and bactericidal activity, preventing new growth of germs, drying-drying of the affected areas and reduction of secretions, normalizing tissue function , Metabolism promoting action, menstrual metabolism promotion, nitrogen metabolism promotion, hematopoietic action can be absorbed as the raw material of blood composition by the same chemical structure of porpyrine nucleus such as hemoglobin of animal. In fact, the clinical trials of anemia showed that recovery was markedly accelerated, and the number of erythrocytes or hemoglobin was increased by 10% and leukocytes increased by 40%. In addition, bactericidal and bactericidal activity is not directly sterilized by the bactericidal chlorophyll based on cell revival, but revives biological tissues, strengthens resistance and indirectly prevents the growth of bacteria. While commonly used chemical fungicides directly attack pathogens, they also disrupt normal cells, but chlorophyll strengthens natural healing powers, strengthens normal cell function, and improves constitution, thereby fundamentally treating diseases. That is, it is characterized by exhibiting a complex effect. To promote pancreatic function Anti-cancer activity is based on treatment and prevention of cancer, removal of carcinogens and neutralization effect. Studies on the removal of carcinogens by administration of Chlorophyll resulted in three carcinogens resulting from dietary intake: heterocyclic amines, smoked or barbecues, mainly derived from cooked meat. It has been reported to bind and remove polycyclic hydrocarbons from food and aflatoxin from mold generated from food (J. of National Cancer Institute, Jan. 4, 1995).

Anti-ulcer action top. There were duodenal ulcer, hyperacidity, chronic gastritis and pancreatitis, and anti-cholesterol and antiallergic activity. (Pharmaceutical Newspaper. 2001.12.2)

Chlorophyll derivatives, chlorophyllin, sold as an OTC drug called Derifil, can significantly reduce the incidence of liver cancer caused by aflatoxin, a fungal toxin, due to reduced aflatoxin-DNA damage. . Patricia A. Eggner, Ph.D., of Johns Hopkins University, said in a paper scheduled for publication in the December 4 issue of the National Academy of Sciences. Chlorophyllin in healthy adults has resulted in a significant reduction in aflatoxin-DNA damage. High levels of aflatoxin-DNA damage are known to increase the risk of developing liver cancer. Aflatoxins are carcinogens produced by fungi found in beans, peanuts, corn and grains. Currently, 'de refill' (chlorophyllin) is sold as a drug that suppresses body odor and fecal odor in elderly patients. So far, chlorophyllin has been shown to inhibit the action of carcinogens in the liver of rodents such as rats. Eggner's team tested 180 healthy Chinese adults to determine if chlorophyllin had the same effect on humans. We chose China as a research site because of the fact that liver cancer has become one of the most frequent cancers in developing countries. In fact, the Chinese are known to consume large amounts of food contaminated with aflatoxin. Participants were asked to take 100 mg of chlorophyllin or placebo three times daily for four months. Three months later, urine samples were tested to look for aflatoxin-DNA damage. As a result, the aflatoxin-DNA damage was 55% lower in the chlorophyllin group than in the placebo group. This suggests that chlorophyllin may help reduce the incidence of liver cancer. Dr. Egner said that taking chlorophyllin or eating foods rich in chlorophyll may be an effective way to suppress the development of liver cancer and other cancers caused by environmental factors. In this regard, the fact that green chlorophyll, abundant in green plants, reduces the incidence of cancer in animals has been demonstrated in a series of previous studies.

The first person to prove the efficacy of wheat, barley and grain sprouts in experiments was Schnabel, an American chemist in 1928. Popularity in the United States has begun to hit the Hippocrates Health Association, with diabetes, hypertension, obesity, gastritis, gastric ulcers, pancreas, liver disease, asthma, glaucoma, eczema, skin diseases, constipation, hemorrhoids, diverticulitis, colitis, Arthritis, anemia, bad breath, women's diseases, and was known to have been fixed. Dr. Hagiwarayoshihide, a Japanese nutritionist, studied more than 150 species of plants and found that barley sprouts had the most excellent effect in preventing disease. Barley sprout contains more than 55 times of potassium milk, 18 times of spinach, and 11 times of calcium in milk, 5 times more than spinach to prevent anemia, vitamin seeds 3 times more than spinach, 60 times more than apples, adult diseases. Contains a large amount of superoxide dismutase (SOD), an enzyme that breaks down free radicals

Cocoa is rich in antioxidants (polyphenols) that are effective in removing the body's substances that can damage cells and cause cancer, which is good for preventing aging and preventing cancer. Cacaopolyphenols have an immunomodulatory effect that prevents colds and suppresses allergies. Caffeine lightens the central nervous system and brightens the feeling of stagnation, while chocolate sugar softens the nerves, which is good for fatigue, anxiety and nervousness. It is effective in preventing caries because it contains cacaopolyphenol, a substance that prevents tooth decay.

The effect of rice bran is that the rice bran contains amazing nutrients. Nowadays, rice bran is mainly used for livestock feed such as chicken or pig. The eggs laid by chickens that have been fed rice bran are shiny and taste great. The rice bran is rich in various nutrients. Rice bran is a pulverized mixture of skin, seedling and whistle layer from the process of turning from brown rice to white rice.

Thus, rice bran is a by-product of polished rice, but it is an excellent ingredient in terms of nutrition.

Rice bran contains vitamins A, B1, B6, iron, phosphorus and minerals.

The standard chemical composition of rice bran is 13.5% moisture, 18.3% fat, 38.3% sugar, 7.8% fiber, and 8.9% ash.

Vitamin B1 contains 2.5mg of 100g, and vitamin E is also abundant.Eating rice bran will increase the activity of the intestines due to the fiber from plants, which will promote bowel movements, which can solve even the most constipation. In addition, plant fiber also prevents colon cancer because it increases the bifidus bacteria in the intestine and maintains the balance of the bacteria parasitic in the intestine.

Increasing Bifidobacterium makes the intestines healthy and plays an important role in preventing aging. It is also effective in preventing fatty liver. Rice bran is also a good food for people who are prone to stones because it acts to inhibit the absorption of calcium in the small intestine. Put rice bran in a pouch made of cotton or silk cloth to wipe your face and skin, leaving your face shiny and your body surprisingly clean. The cleaning effect of soap will not be comparable. Also, if you wipe your body with rice bran, you can keep your skin beautiful and shiny. It is also effective in treating skin diseases. In particular, rice bran has been used as a folk remedy for the treatment of chronic skin diseases with itching. Atopic dermatitis is a representative of chronic skin diseases with itching. Sometimes people with this skin condition actually use rice bran pouches instead of soap. Why does rice bran beautify skin and improve skin disease? The reason is that the action of vitamin B1, B6, E, etc. contained in rice bran. Therefore, it is announced that the effect is excellent in terms of health and beauty.

The rice bran contains not only GABA, which has a lot of effects on the brain in our body, but also consists of many soil microorganisms and its own enzymes, and a treasure trove of soil microorganisms, as well as the microorganisms we need to make meju or Cheonggukjang. It contains a lot of microorganisms that are beneficial to humans, and especially penicillium, a microorganism that makes penicillin, which can neutralize powerful antimicrobial substances in cocoa shells. In addition, it is believed that functional substances of secondary metabolites can also be obtained by fermenting and mixing several microorganisms of Bacillus subtilis and rare actinomycetes.

On the other hand, the microorganisms used in the fermentation is an important value in the biomaterials industry because of its high added value is recognized as useful. Many countries around the world are using microorganisms to search for new substances that can be used in various ways, such as antibiotics, anticancer drugs, pesticides, insecticides, herbicides, pesticides and health supplements, and food materials such as enzymes. To date, more than 10,000 new substances have been discovered from microorganisms, and about 100 new substances have already been put into practical use.

Among the microorganisms, Bacillus subtilis to be used in the present invention is an aerobic by-product bacillus and is a very small microorganism of less than 1 μm in diameter. Sugars are the carbon source, and ammonium salts are the nitrogen source when making antibiotics. The fungus is not used to cure illnesses, but rather as a fungicide for odor or canning. Actinomycetes are also recognized as the most important microorganisms in the industry due to the diversity of chemical structures and species abundance, especially in secondary metabolites. About two-thirds of the 10,000 bioactive substances found so far from actinomycetes Derived. Actinomycetes are expected to occupy an important position in the biomaterials industry through the isolation of new strains and gene function.

On the other hand, actinomycetes have been classified as fungi in the past because they form fungus-like forms, but as the physiological and biochemical analysis methods have been developed since the 1970s, they are recognized as protozoa with high GC content and differentiation properties. Prior to the 1960's, Streptomyces sp.

Later, when gentamycin was discovered from actinomycetes of Micromonospora, rare actinomycetes other than the genus Streptomyces began to receive much attention. In 1989 Bergey's Manual of Systematic Bacteriology (1) describes 40 species of rare actinomycetes.

The present invention mixes chlorophyll-rich young barley sprouts, young wheat sprouts and young oat sprouts with rice bran and undergoes primary fermentation with fermentation microorganisms present in rice bran and chlorophyll itself. Fermented by mixing sorghum by-products and tertiary fermentation using various micro-medicinal herbs, natural nutrients, natural vitamins, minerals, algae, grains and soybeans, additives, vegetable fats and fats, fermented with fermented microorganisms, and then animal feed additives. It relates to a fermentation product which can be used as fermented herbal medicine raw materials, food raw materials, cosmetic raw materials, bioactive substances using secondary metabolites and other additives and the like and methods for producing the same.

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The present invention mixes chlorophyll-rich young barley sprouts, young wheat sprouts, and young oat sprouts with rice bran, followed by primary fermentation with fermentation microorganisms present in rice bran and chlorophyll itself, and then crushed cocoa shells and sugarcane by-products extracted from raw sugar. Secondary fermentation and animal feed by fermentation extracts prepared after fermentation using fermented microorganisms by adding natural nutrients, nutrients, herbs, minerals, algae, grains and legumes, additives, vegetable oil and fats It relates to a fermentation extract that can be used as additives, fermented herbal medicine raw materials, raw materials of food, cosmetic raw materials, bioactive substances using secondary metabolites and other additives, and a method of manufacturing the same.

The present invention is a barley, wheat germ, oat sprouts one or more chlorophyll selected from the rapid freeze dried and then pulverized to 70 ~ 200 mesh and mixed with rice bran and distilled water to make a water content of about 9 to 15% step; Primary fermentation of the rice bran mixture with the activity of enzymes and fermentation microorganisms present in rice bran and chlorophyll itself; Secondary fermentation in an environment to sterilize and dry the fermentation products after the primary fermentation, and then crush and mix the crushed cocoa shells and the raw sugar extracted sugar cane to ferment the mixture of microorganisms and carbon sources in the sugar cane. Making a step; Sterilizing and drying the fermented product after the secondary fermentation, and then mixing a natural nutrient, nutritional substance, herbal medicine, minerals, algae, grains and legumes, additives, and vegetable fat or oil to prepare a mixed medium; Tertiary fermentation by adding fermentation microorganisms to the mixed medium; It is characterized in that the fermentation extract of chlorophyll containing a chlorophyll consisting of an extraction step of sterilizing and drying the fermentation product of the third fermentation process and then mixed, stirred and extracted in an aqueous alcohol solution.

In the present invention, chlorophyll-rich barley sprouts, wheat sprouts and oat sprouts are mixed with rice bran for the first fermentation, and during the second fermentation, cocoa husks and raw sugar cane by-products are mixed and fermented to produce cocoa husks with strong antibacterial properties. After fermentation, natural nutrients and nutrients, herbals, minerals, algae, grains and soybeans, additives, and vegetable fats and fats are added to the secondary fermentation products. By fermentation extract through the third fermentation process, the molecular size of nutrients or functional substances in the fermentation products fermented in the third fermentation is broken down into very small units from kilodaltons (Kd) to daltons (d). Enhancement and formation of secondary metabolites, resulting in animal feed additives, fermented herbal ingredients, food ingredients, fragrance raw materials, bioactive substances using secondary metabolites and other There is an effect that can be provided as a high additive raw material such as additives.

Figure 1 is a simplified illustration of the production step of fermented extract of rice bran containing chlorophyll according to the present invention.

Hereinafter, the preferred embodiment of the present invention will be described in detail. In the following description of the present invention, if it is determined that the detailed description of the related known function or the like may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted.

The present invention comprises the steps of making a rice bran mixture of one or more chlorophyll selected from barley sprouts, wheat sprouts, oat sprouts and then crushed into 70-200 mesh and mixed with rice bran and distilled water to a water content of about 9-15%; Primary fermentation of the rice bran mixture with the activity of enzymes and fermentation microorganisms present in rice bran and chlorophyll itself; Secondary fermentation in an environment to sterilize and dry the fermentation products after the primary fermentation, and then crush and mix the crushed cocoa shells and the raw sugar extracted sugar cane to ferment the mixture of microorganisms and carbon sources in the sugar cane. Making a step; Sterilizing and drying the fermented product after the secondary fermentation, and then mixing a natural nutrient, nutritional substance, herbal medicine, minerals, algae, grains and legumes, additives, and vegetable fat or oil to prepare a mixed medium; Tertiary fermentation by adding fermentation microorganisms to the mixed medium; The fermentation product of the third fermentation process is sterilized and dried, and then mixed with an aqueous alcohol solution, followed by an extraction step of extracting by stirring to prepare a fermentation extract of chlorophyll-containing rice bran.

This will be described in more detail as follows.

One or more chlorophylls selected from barley, wheat and oat sprouts are dried and pulverized and mixed with rice bran and distilled water to form a rice bran mixture with a water content of 9-15%.

Barley, wheat, and oat sprouts are 5 ~ 50㎝ in size when the green is the darkest. Quick freeze-dried and crushed into 70 ~ 200 mesh before use. The young shoots contain 55 times more potassium milk and 18 times more spinach, 11 times more calcium than milk, 5 times more than spinach to prevent anemia, vitamin seeds 3 times more than spinach, 60 times more than apples, and free radicals. It contains a lot of enzyme superoxide dismutase (SOD).

The powdered rice bran mixture is made into koji, and the fermentation is carried out so that white mold covers the whole koji with the activity of enzymes and fermentation microorganisms present in chlorophyll and rice bran itself in an incubation in which humidity is automatically controlled.

In other words, aerobic aerobic mixture of chlorophyll and rice bran in a fermentation temperature of 18 ~ 50 ℃, humidity 50 ~ 85%, pH 5 ~ 6.7 suitable moisture content 9 ~ 15%, an environment in which a large amount of beneficial microorganisms can ferment The fermentation takes place by providing the environment for 2 to 30 days in an intermediate and anaerobic state.

The fermentation product is finished, sterilized, and dried.

That is, the first fermented fermentation product is used to sterilize the sanitary part due to excessive growth in the fermentation process of the microorganisms used in the fermentation and to use the rapid sterilizer (autocraver) to prevent the interaction of other bacteria in the second fermentation operation After sterilization for 10 minutes to 10 hours at 80 ~ 132 ℃ and the drying process so that the moisture content in the dryer 7 ~ 15%.

Crushing and mixing the crushed cocoa shells and sugar cane in the dried primary fermentation product is subjected to secondary fermentation to ferment with microorganisms and sugars and carbohydrates (carbon source, nitrogen source) that are rich in sugar cane.

In other words, fermentation of cocoa bark powder with strong antimicrobial properties is made by mixing sugarcane (raw sugar extracted) powder that can activate its own microorganisms to make a secondary fermentation cozy, and the fermentation temperature in an automatically controlled incubation 18 At ~ 50 ℃, humidity 50 ~ 85%, pH 5 ~ 6.7, moisture content 9 ~ 15%, fermentation is provided for 2-30 days in the middle of aerobic and anaerobic conditions.

The second fermented fermentation product is rapidly sterilized and dried, which means that the fermented product after the first fermentation is sanitary due to overgrowth in the fermentation process of the microorganisms used in the fermentation and other germs during the second fermentation. In order to prevent the conflict, sterilize at 80 ~ 132 ℃ for 10 minutes ~ 10 hours using a rapid sterilizer (autocraver) and dry it so that the moisture content in the dryer is 7 ~ 15%.

Natural nutrients, nutritional substances, herbal medicines, minerals, algae, grains and soybeans, additives, vegetable oil and fat degreasing portion of the dried secondary fermentation products by mixing the secondary fermentation product weight parts 5 to 85 or 85 to 5 medium Make

The natural nutrients; Spirulina extract, chlorella extract, noni extract, palm extract, cocoa extract, high test molasses, refined molasses, fish meal, beer yeast, animal meat extract, yeast extract, potato extract, turmeric extract, mango extract, asparagine extract, casein, skim milk Select one or more of the

Vitamins, dietary fibers, amino acids are used as the nutrients

Vitamins of the nutrients are selected from one or more of vitamins A, B₂, B, B12, C, D₂, D₃, E, K and folic acid,

As dietary fiber among the nutrients, one or more selected from chafer blood, cellulose, hemicellulose, crystalline cellulose, lignin, pectin, alginic acid, guar gum, gum arabic, konjac mannan, inulin, polydextrose, maltodextrin,

Among the nutrients, amino acids are selected from one or more of histidine, isoleucine, leucine, lysine, methionine, valine, cystine, tryptophan, threonine, phenyl, alanine and tyrosine.

The herbal medicines, root, brown, licorice, liquor, health, dryness, gumsil, gilyeong, gyeolja, gyeji, cinnamon, red ginseng, grain, bale, gugyang, fruit fruit, lump, gojija, gujeolcho, gupan, guin, chrysanthemum , Silver and gold coins, moth, wild root, noni, green tea, deer antler, dansam, donkey, gingko, daikon, daebokpi, rhubarb, gongyanghyang, daepok, daesan, contrast, deity, poisonous, confectionery, donggyuja, cordyceps, dongchung, mazain, ephedra , Net, man-shaped, malt, ganmun-dong, cotton yarn, quince, seedling, bark skin, neck, cedar, myrrh, peppermint, half, airproof, windproof, white nap, white porcelain, white paper, baekchul, golgol, bokyeong, bokbunja, loquat , Vitamins, samsam, casualties, autographs, hawthorn, cornus, medicinal herbs, sanjoin, sancho, sangsang, baekbaekpi, upper leaves, situation mushrooms, ginger, stone name, stone valley, stone changpo, sesin, joiner, element, songhwabun, horse riding, shiho, new song , God, acaricus, eoseongcho, love, journey room, lotus root, farewell, youngsil, manor, ogapi, ume, schisandra, Song, allies, dew, fingering, turmeric, original paper, lieutenant ship, breast milk, baekbaekpi, frankincense, nutmeg, breeding, yinyangkok, ripe, honeysuckle, ginseng, injinho, jaso, zahager, peony, ghost, scorpion, kill, kill, Bamboo leaf, cloves, phalanges, earthly, fruit room, fat milk, yeolhwang, dermis, tired person, green tea, tea, changja, creation, cheonnamseong, cheongungung, cheonma, astronomical dong, egret, incense, gardenia, coffee, taxa, earthenware, rupture , Green onion, seaweed, pogongyoung, aspirations, shiitake, hagocho, sewage, thawed blood, passerby, hyangbuja, balm, Hyunsam, Hyunho color, hyungae, golden, yellow white, yellow lotus, yellow flower, hwangchi, hop, red ginseng, safflower, black pepper, pepper Choose one or more of, rare, fennel, black bean, and black axis.

The minerals include germanium, selenium, copper, magnesium, manganese, molybdenum, zinc, iodine, iron, calcium, potassium, chromium, uranium, pegmatite, elvan, mica, woodstone, illite, graphite, gold, and silver. Choose above.

In the alga, brown seaweed, gompi, seaweed, snails, kelp, stone starfish, swelling, messinyi, seaweed, spirulina. Choose one or more of Chlorella, Locust, Sprout, Green, Seaweed.

The grains and soybeans may be selected from at least one of rice, glutinous rice, brown rice, nagigi, rice bran, barley, wheat, rye, oats, crude, sorghum, corn, buckwheat, barley, millet.

As the additive, an acidulant, a sweetener, a flavoring agent may be used.

Among the additives, at least one of citric acid, malic acid, tartaric acid, and succinic acid is selected as an acidulant. .

Among the additives, at least one selected from xylitol, sorbitol, maltose, lactose, glucose, fructose, sugar, aspartame and stevio.

Among the additives, the flavoring agent may be selected from at least one of mixed fruit flavor, apple flavor, yogurt flavor, banana flavor, apricot flavor, lemon flavor, rose flavor, and jinjin mugwort flavor.

The vegetable oil skim powder is a by-product degreased from oilseeds, flaxseed skim by-products, mazain skim by-products, olive seed skim by-products, sesame seed skim by-products, cotton seed skim by-products, soybean seed skim by-products, peanut seed skim by-products, rice bran skim by-products Select one or more of the

The fermentation microorganism is added to the mixed medium for tertiary fermentation.

The fermentation microorganism in the present invention is a bacterium used as the fermentation microorganism in the third fermentation step so as to ferment the raw materials with high antimicrobial activity smoothly, Bacillus subtilis genus, Aspergillus genus, Actinomycetes It consists of mixed bacteria cultured by growing in a medium, but the genus Bacillus subtilis is Bacillus niger, Aspergillus genus Aspergillus orizae (Aspergillus oryzae), Aspergillus sperm (Aspergillus sojae), and actinomycetes include Rare actinomycetes, Microbispora, Streptosporangium, Micromonospora, Nocardioforms, and Dactilosporang. One selected microorganism of the genus Bacillus subtilis in Dactylosporangium and one of two microorganisms of the genus Aspergillus One microorganism out of five Rare actinomycetes was selected and mixed, and the cultured strain was added to the fermentation product at a fermentation temperature of 18 to 50, humidity of 50 to 85%, pH of 5 to 6.7, and moisture content of 9 to 15%. It provides an active fermentation condition by providing the environment for 2 to 30 days in the middle of aerobic and anaerobic conditions.

The fermentation product of the third fermentation work is sterilized for 10 minutes to 10 hours at 80 ~ 132 ℃ using a rapid sterilizer (autocraver) and the drying process so that the water content in the dryer 7 ~ 15%. Mixing and stirring the aqueous alcohol solution to the dried tertiary fermentation product to extract under reduced pressure to a pressure of 1 to 10 Kgf / ㎠ at a temperature of 40 to 90 ℃ to form a fermentation extract, the alcohol solution is distilled water 20 to 80 parts by weight, anhydrous ethanol It is formed in parts by weight of 30 to 70.

The fermentation extract may further include a further aging step of aging for 10 to 100 days at a temperature of 5 to 10 ℃.

Fermented extracts made of powders and extracts made by the above process will be manufactured with animal feed additives, fermented herbal medicine raw materials, food raw materials, fragrance raw materials and other additives.

[Example]

Chlorophyll-rich barley, wheat, and oat sprouts are dried to 5-50cm in size, rapidly freeze-dried, crushed into 70-200 mesh, and mixed with rice bran and water content of 9-15%. Making step (S10),

Fermentation temperature 18 ~ 50 ℃, humidity 50 ~ 85%, pH 5 ~ 6.7 suitable for fermentation of a large amount of beneficial microorganisms in an incubation in which the rice bran mixture is made into powdery koji, the humidity is automatically controlled First fermentation for 2-30 days in a semi-aerobic state at a water content of 9-15% (S20),

In order to prevent the hygiene part due to excessive growth in the fermentation process of the microorganisms used in the fermentation of the mixed fermentation product of the first fermentation and other bacteria in the second fermentation operation, a rapid sterilizer (autocraver) is used. Sterilize at 80 ~ 132 ℃ for 10 minutes ~ 10 hours, dry in a drier to reach 7 ~ 15% water content, and then mix the first fermented product with cocoa shell powder and sugarcane by-product powder. In fermentation temperature 18 ~ 50 ℃, humidity 50 ~ 85%, pH 5 ~ 6.7 in aerobic and anaerobic intermediate state at moderate moisture content 9 ~ 15% Secondary fermentation step of solid powder (S30), which provides the environment for 2-30 days

The secondary fermentation product was sterilized at 80 to 132 ° C. for 10 minutes to 10 hours using a rapid sterilizer (autocraver) and dried in a drier to achieve a moisture content of 7 to 15%. Making a medium by mixing the product and natural nutrients and nutritional substances, herbal medicines, minerals, algae, grains and soybeans, additives, vegetable oil and skim flour 5 to 85 or 85 to 5 parts by weight (S40),

The natural nutrients, spirulina extract, chlorella extract, noni extract, palm extract, cocoa extract, high test molasses, refined molasses, fish meal, beer yeast, animal meat extract, yeast extract, potato extract, turmeric extract, mango extract, asparagine Select one or more of X, casein and skim milk,

Vitamins of the nutrients are selected from at least one of vitamins A, B₂, B, B12, C, D₂, D₃, E, K and folic acid,

The dietary fiber of the nutritional substance is selected from at least one of cha cha blood, cellulose, hemicellulose, crystalline cellulose, lignin, pectin, alginic acid, guar gum, gum arabic, konjac mannan, inulin, polydextrose, maltodextrin,

The amino acids in the nutritional substance is at least one selected from histidine, isoleucine, leucine, lysine, methionine, valine, cystine, tryptophan, threonine, phenyl, alanine, tyrosine.

The herbal medicines are brown root, brown flower, licorice, vigor, health, dry rate, gumsil, gilyeong, gyeolja, gyeji, cinnamon, red ginseng, grain, bale, kwakhyang, fruit fruit, lump, gojija, gujeolcho, gupan, guin, chrysanthemum, gold and silver , Lobster, Root, Noni, Green Carving, Deer Antler, Dansam, Angelica, Dangsam, Macaque, Daebokpi, Rhubarb, Conventional Fragrance, Daegu, Daesan, Contrast, Doin, Dokki, Confectionery, Donggyuja, Cordyceps Sinensis Fruit, mantis, malt, malmungdong, cotton yarn, quince, currant, bark skin, neck, cedar, myrrh, peppermint, half, half, airproof, windproof, white nap, white porcelain, white paper, baekchul, golgol, bokyeong, bokbunja, loquat leaf, vitamin Tree, samsam, casualty, autograph, hawthorn, cornus, medicinal herb, sanjoin, sancho, sangsang, sangbaekpi, evergreen, situation mushroom, ginger, stone name, stone valley, stone changpo, seshin, joiner, element, songhwabun, horse riding, shiho, new song, god , Acaricus, eosungcho, love, journey room, lotus root, junior high school, manor room, manor, ogapi, ume, omeza, wa , Alligator, dew, fingering, turmeric, garden, lieutenant, breast milk, milky skin, frankincense, nutmeg, breeding, yin and yang, acquaintances, honeysuckle, ginseng, injinho, jasmine, zahager, peony, ghost, scorpion, kill, death, bamboo leaf , Cloves, phalanges, earthly, fiery, fat, jihwang, dermis, tired, green tea, tea, changja, creation, cheonnamseong, cheongung, cheonma, astronomical dong, egret, incense, gardenia, coffee, taxa, earthenware, rupture, Green onion, green onion, pogongyoung, aspirations, shiitake, hagocho, sesao, thaedipi, passerby, hyangbuja, balm, Hyunsam, Hyunho color, hyungae, golden, yellow white, yellow lotus, yellow flower, hwangchil, hop, red ginseng, safflower, black pepper, pepper, Choose one or more of rare, fennel, black bean, and black axis.

The minerals include germanium, selenium, copper, magnesium, manganese, molybdenum, zinc, iodine, iron, calcium, potassium, chromium, uranium, pegmatite, elvan, mica, woodstone, illite, graphite, gold, and silver. Choose above.

Types of algae in the algae, gompi, seaweed, stalks, kelp, stony, squirting, messinyi, seaweed, spirulina. Choose one or more of Chlorella, Locust, Sprout, Green, Seaweed.

The grains and soybeans are selected from at least one of rice, glutinous rice, brown rice, nagigi, rice bran, barley, wheat, rye, oats, crude, sorghum, corn, buckwheat, yulmu, and millet.

The additive is an acidulant, a sweetener, a flavoring agent, but the acidic agent is selected from citric acid, malic acid, tartaric acid, succinic acid. As a sweetener, one or more of xylitol, sorbitol, maltose, lactose, glucose, fructose, sugar, aspartame and stevio is selected, and the flavoring agent is mixed fruit flavor, apple flavor, yogurt flavor, banana flavor, apricot flavor, lemon flavor, rose flavor Choose one or more of jinjin scent.

The vegetable oil skim powder is a by-product degreased from oilseeds, flaxseed skim by-products, mazain skim by-products, olive seed skim by-products, sesame seed skim by-products, cotton seed skim by-products, soybean seed skim by-products, peanut seed skim by-products, rice bran skim by-products Select one or more of the

The fermentation microorganism is added to the mixed medium through the above step to carry out the third fermentation, and the species used as the fermentation microorganism are Bacillus subtilis genus, Aspergillus genus, and actinomycetes grown in a single medium cultured. Bacillus subtilis genus is Bacillus niger, Aspergillus genus Aspergillus oryzae, Aspergillus sojae (Aspergillus sojae), rare as actinomycetes Bacillus among Microbispora, Streptosporangium, Micromonospora, Nocardioforms, Dactylosporangium among Rare actinomycetes one microorganism of the genus subtilis and one of two species of the genus Aspergillus and one of five of the rare actinomycetes. Selected and mixed with water, the cultured strain is added to the fermentation product, and the incubation temperature and humidity is automatically controlled in fermentation temperature 18 ~ 50 ℃, humidity 50 ~ 85%, pH 5 ~ 6.7 aerobic at a suitable moisture content 9 ~ 15% Undergoing the third fermentation process for 2 to 30 days in an anaerobic and anaerobic state (S50),

After the third fermentation, the fermented product is sterilized at 80 to 132 ° C. for 10 minutes to 10 hours using a rapid sterilizer (autocraver), and dried in a drier to obtain a moisture content of 7 to 15%. The aqueous solution of alcohol is mixed with the dried tertiary fermentation product, stirred and extracted under reduced pressure at a pressure of 1 to 10 Kgf / cm 2 at a temperature of 40 to 90 ° C. to extract the fermentation composition. The aqueous alcohol solution is formed of 20 to 80 parts by weight of distilled water, 30 to 70 parts by weight of anhydrous ethanol, extraction step of extracting (S60),

The fermentation composition of the extracted includes a further aging step of aging for 10 to 100 days at a temperature of 5 to 10 ℃. (S70)

While the present invention has been described with reference to the exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments.

S10: step of making rice bran mixture
S20: first fermentation stage
S30: 2nd fermentation stage
S40: step of making mixed medium
S50: 3rd fermentation stage
S60: fermented extract extraction step
S70: ripening step

Claims (12)

Lyophilizing one or more chlorophylls selected from barley, wheat and oat sprouts, pulverizing to 70-200 mesh, mixing rice bran and distilled water to form a rice bran mixture that is mixed with water content of about 9-15%;
Primary fermentation of the rice bran mixture with the activity of enzymes and fermentation microorganisms present in rice bran and chlorophyll itself;
Secondary fermentation in an environment to sterilize and dry the fermentation products after the primary fermentation, and then crush and mix the crushed cocoa shells and the raw sugar extracted sugar cane to ferment the mixture of microorganisms and carbon sources in the sugar cane. Making a step;
Sterilizing and drying the fermented product after the secondary fermentation, and then mixing a natural nutrient, nutritional substance, herbal medicine, minerals, algae, grains and legumes, additives, and vegetable fat or oil to prepare a mixed medium;
Tertiary fermentation by adding fermentation microorganisms to the mixed medium;
The fermentation extract production method of chlorophyll containing rice bran chlorophyll, characterized in that consisting of; the sterilization and drying the fermentation product of the third fermentation process, followed by mixing and stirring in an aqueous alcohol solution.
The method of claim 1,
In the first fermentation step,
The rice bran mixture is fermented in an incubator at a fermentation temperature of 18 to 50 ° C., a humidity of 50 to 85%, a pH of 5 to 6.7 and a suitable moisture content of 9 to 15% for 2 to 30 days in an aerobic and anaerobic medium. Fermented extract manufacturing method of rice bran containing chlorophyll.
The method of claim 1,
In the second fermentation step,
Sterilize the primary fermentation product at 80 ~ 132 ℃ for 10 minutes ~ 10 hours using sterilizer and dry it to 7 ~ 15% of water content in the dryer, then fermentation temperature 18 ~ 50 ℃, humidity 50 ~ 85%, pH 5 ~ 6.7 Method for producing a fermented extract of chlorophyll containing rice bran, characterized in that the fermentation for 2-30 days in aerobic and anaerobic intermediate state at a suitable moisture content of 9-15%.
The method of claim 1,
In the step of making the mixed medium,
Sterilize the secondary fermentation product at 80 ~ 132 ℃ for 10 minutes ~ 10 hours using sterilizer and dry it to 7 ~ 15% of water content in the dryer, then natural nutrients, nutrients, herbal medicines, minerals, algae, grains and A method for producing fermented extract of chlorophyll containing rice bran, characterized in that by mixing the secondary fermentation product weight parts 5 to 85 or 85 to 5 of the soybeans, additives, vegetable fat and oil degreasing.
The method of claim 4, wherein
The natural nutrients; Spirulina extract, chlorella extract, noni extract, palm extract, cocoa extract, high test molasses, refined molasses, fish meal, beer yeast, animal meat extract, yeast extract, potato extract, turmeric extract, mango extract, asparagine extract, casein, skim milk Select one or more of
The nutritional substance uses vitamins, dietary fibers, amino acids,
The herbal medicines, root, brown, licorice, liquor, health, dryness, gumsil, gilyeong, gyeolja, gyeji, cinnamon, red ginseng, grain, bale, gugyang, fruit fruit, lump, gojija, gujeolcho, gupan, guin, chrysanthemum , Silver and gold coins, moth, wild root, noni, green tea, deer antler, dansam, donkey, gingko, daikon, daebokpi, rhubarb, gongyanghyang, daepok, daesan, contrast, deity, poisonous, confectionery, donggyuja, cordyceps, dongchung, mazain, ephedra , Net, man-shaped, malt, ganmun-dong, cotton yarn, quince, seedling, bark skin, neck, cedar, myrrh, peppermint, half, airproof, windproof, white nap, white porcelain, white paper, baekchul, golgol, bokyeong, bokbunja, loquat , Vitamins, samsam, casualties, autographs, hawthorn, cornus, medicinal herbs, sanjoin, sancho, sangsang, baekbaekpi, upper leaves, situation mushrooms, ginger, stone name, stone valley, stone changpo, sesin, joiner, element, songhwabun, horse riding, shiho, new song , God, acaricus, eoseongcho, love, journey room, lotus root, farewell, youngsil, manor, ogapi, ume, schisandra, Song, allies, dew, fingering, turmeric, original paper, lieutenant ship, breast milk, baekbaekpi, frankincense, nutmeg, breeding, yinyangkok, ripe, honeysuckle, ginseng, injinho, jaso, zahager, peony, ghost, scorpion, kill, kill, Bamboo leaf, cloves, phalanges, earthly, fruit room, fat milk, yeolhwang, dermis, tired person, green tea, tea, changja, creation, cheonnamseong, cheongungung, cheonma, astronomical dong, egret, incense, gardenia, coffee, taxa, earthenware, rupture , Green onion, seaweed, pogongyoung, aspirations, shiitake, hagocho, sewage, thawed blood, passerby, hyangbuja, balm, Hyunsam, Hyunho color, hyungae, golden, yellow white, yellow lotus, yellow flower, hwangchi, hop, red ginseng, safflower, black pepper, pepper Choose one or more of the following: rare, fennel, black bean, black shaft,
The minerals include germanium, selenium, copper, magnesium, manganese, molybdenum, zinc, iodine, iron, calcium, potassium, chromium, uranium, pegmatite, elvan, mica, woodstone, illite, graphite, gold, and silver. Choose more than
In the alga, brown seaweed, gompi, seaweed, snails, kelp, stone starfish, swelling, messinyi, seaweed, spirulina. Choose one or more of chlorella, woodfish, 톳, green, seaweed,
In the cereals and beans, one or more of rice, glutinous rice, brown rice, raggi, rice bran, barley, wheat, rye, oats, crude, sorghum, corn, buckwheat, yulmu, millet,
The additives include acidulants, sweeteners, flavoring agents,
The vegetable oil skim powder is a by-product degreased from oilseeds, flaxseed skim by-products, mazain skim by-products, olive seed skim by-products, sesame seed skim by-products, cotton seed skim by-products, soybean seed skim by-products, peanut seed skim by-products, rice bran skim by-products Fermented extract manufacturing method of rice bran containing chlorophyll, characterized in that at least one selected from.
6. The method of claim 5,
Vitamins of the nutrients are selected from at least one of vitamins A, B₂, B, B12, C, D₂, D₃, E, K and folic acid,
The dietary fiber of the nutritional substance is selected from at least one of cha cha blood, cellulose, hemicellulose, crystalline cellulose, lignin, pectin, alginic acid, guar gum, gum arabic, konjac mannan, inulin, polydextrose, maltodextrin,
Amino acids in the nutritional substance prepared by fermentation extract of chlorophyll containing chlorophyll, characterized in that at least one selected from histidine, isoleucine, leucine, lysine, methionine, valine, cystine, tryptophan, threonine, phenyl, alanine, tyrosine Way.
6. The method of claim 5,
One or more selected from citric acid, malic acid, tartaric acid, succinic acid as the acidulant of the additive,
Among the additives, at least one selected from xylitol, sorbitol, maltose, lactose, glucose, fructose, sugar, aspartame, stevio,
Among the additives, the flavoring agent is a mixed fruit flavor, apple flavor, yogurt flavor, banana flavor, apricot flavor, lemon flavor, rose flavor, jinjin mugwort flavor, characterized in that at least one selected from chlorophyll-containing rice bran fermentation extract manufacturing method.
The method of claim 1,
In the third fermentation step,
Bacillus species used as fermented microorganisms are composed of Bacillus subtilis genus, Aspergillus genus, and actinomycetes grown in one medium.
The genus Bacillus subtilis is Bacillus niger, Aspergillus genus Asgillus oryzae, Aspergillus sojae, and actinomycetes as rare actinomycetes (Rare actinomycetes) One of the genus Bacillus subtilis among Microbispora, Streptosporangium, Micromonospora, Nocardioforms, and Dactylosporangium One microorganism of the selected microorganism and two species of the genus Aspergillus and one microorganism of five rare actinomycetes are selected and mixed, and the cultured strain is added to the fermentation product and the fermentation temperature is 18 to 50 Fermentation of chlorophyll-containing rice bran, characterized in that fermentation is carried out for 2-30 days in an aerobic and anaerobic medium at a humidity of 50 to 85%, a pH of 5 to 6.7, and a water content of 9 to 15%. Chulmul method.
The method of claim 1,
In the extraction step,
The fermented product after the third fermentation is sterilized at 80-132 ℃ for 10 minutes to 10 hours using a sterilizer, and an alcoholic solution is added to the dried tertiary fermented product so that the moisture content is 7-15% in the dryer. Method for producing a fermented extract of chlorophyll containing chlorophyll, characterized in that the extraction under reduced pressure at a pressure of 1 to 10 Kgf / ㎠ at a temperature of 40 to 90 ℃ by mixing and stirring.
The method of claim 9,
The aqueous solution of alcohol is 20 to 80 parts by weight of distilled water, ethanol anhydrous ethanol by weight of 30 to 70 is characterized in that the chlorophyll-containing fermented rice production method.
The method of claim 1,
Method for producing a fermented extract of chlorophyll containing chlorophyll, characterized in that it further comprises the step of ripening the fermentation extract at a temperature of 5 to 10 ℃ for 10 to 100 days.
Fermented extract of chlorophyll containing chlorophyll prepared by any one of claims 1 to 11

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