JPH05146272A - Plant antioxidant composition - Google Patents

Abstract

PURPOSE:To obtain the subject composition capable of orally taking and suppressing an active enzyme which was excessively produced, excellent in maintenance and promotion of health and useful for prevention and treatment of disease by adding a crushed material of oil seed to a specified fermented and crushed raw material and granulating the resultant blend. CONSTITUTION:The objective composition is obtained by adding crushed material of oil seed such as sesame and crushed material of a plant body selected from tea leaves, green leaves and Chinese lemon containing vitamin C or a vitamin C derivative to fermented and crushed raw material obtained by blending powders obtained by each independently roasting, pulverizing, cooking, fermenting and drying plant seeds selected from plural kinds of soybeans, adlay, wheat and rice and embryo adlay or saccharide of plant seed granulating the blend.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a plant antioxidant composition, and more particularly to a plant antioxidant composition used for maintaining and promoting human health and preventing and treating diseases.

[0002]

2. Description of the Related Art Recent causes of so-called adult diseases such as arteriosclerosis, cancer, heart disease and diabetes have been studied in fields such as medical science and biochemistry. It is said that it is caused by "oxygen"), and its research is progressing. This active oxygen has both merit and detriment effects in the living body. That is, active oxygen is responsible for the defense mechanism against infection of the living body, but uncontrolled excess active oxygen is generated by the action of nucleic acid or protein denaturation, lipid peroxidation, etc. It is a cause of diseases such as intractable diseases, and has become a serious problem as an oxidative effect of the living body. An enzyme that effectively suppresses such so-called oxygen toxicity is produced in the body, and one of them is superoxide dismutase (Superoxide dismutase).
mutase) (hereinafter abbreviated as SOD). Although a method for treating a disease to which this SOD is administered is also taken, it is only an injection drug and has a duration of about 10 minutes as a medicinal effect, and its effect as an internal medicine has not been proved.
Moreover, of the four types of active oxygen (there are also six types of active oxygen involved in the peroxidation reaction), it is effective and limited only to lesions caused by SOD deficiency.

For example, JP-A-61-2224791 discloses that
An active oxygen-suppressing composition obtained by roasting plant seeds or germs thereof, then adding a microorganism to ferment, and adding vegetable oil obtained from the roasted plant thereto, or adding vitamin C to this composition Is proposed. Moreover, green tea powder is mixed with brown rice powder and yellow powder roasted and ground in Japanese Patent Publication No. 61-29711, and a small amount of koji mold is added to the mixture to make a powder raw material. It describes a method for producing a plant-based nutritional supplement in which raw materials are immersed for about 4 days to extract the active ingredient into mixed oil.

Although the active oxygen depressants disclosed in these publications exert a certain effect, the present situation is that there is a demand for a composition having a highly effective biological antioxidant effect which can be taken orally. is there.

[0005]

In view of such circumstances, an object of the present invention is to provide a biological antioxidant composition which can be orally ingested and has an excellent ingestion effect.

[0006]

Means for Solving the Problems As a result of intensive studies, the inventors of the present invention have independently conducted a process of roasting plural kinds of plant seeds and germs or bran of plant seeds, followed by a crushing process, and subsequent steaming. The roasted ground product of oil seeds and the ground product of vitamin C or a derivative containing a vitamin C derivative are added to a fermentation ground material in which all powders that have undergone the process, the subsequent fermentation process, and the subsequent drying process are mixed. Then, it was found that the plant composition obtained by granulating this has a high biological antioxidant action (eliminating action of surplus active oxygen and action of inhibiting peroxidation reaction of lipid), and complete the present invention. I was able to.

Examples of plant seeds used in the biological antioxidant composition of the present invention include rice, wheat, soybean, corn, pigeon barley, red beans and the like. Of these, soybean and pigeon barley are preferably used. As the germ, germs of these plant seeds are exemplified, but wheat germ, rice bran and the like are preferably used. In addition, it is preferable to use a plurality of these.
Suitable combinations of individual plant seeds include soybeans, pigeons,
Wheat germ and rice bran are mentioned, and the amount of each used is exemplified by a mixing ratio of 2: 1: 1: 2 by weight. It is preferable that these raw materials are as high in quality and fresh as possible. In particular, since the germ, especially the bran, is easily oxidized, it is preferable to use the one immediately after rice polishing.

The roasting in the roasting step of the production of the present invention means, as described in the above-mentioned JP-A-61-224791, that the active ingredient in raw materials such as plant seeds is depolymerized to reduce roasting. It refers to giving the heat necessary for molecularization under conditions that do not burn. Each roasting temperature varies depending on the type of plant seeds and heating time, but it is 5
Roast carefully for ~ 6 hours. As the heating method, it is necessary to adopt a means capable of heating uniformly so that the temperature difference between the inner layer and outer layer of particles such as seeds and germs becomes small. For this purpose, heating with far infrared rays, uniform heating with a constant temperature bath, heating with a fluidized bed, etc. are adopted, but it is preferable to use a pot or container such as stone or pottery.

After roasting each plant seed raw material,
In the crushing process, make powder by type. In this case, the powder is not necessarily made into a fine powder because it is pulverized so as to be mixed well with the seed koji in the koji making process. After the crushing process, the roasted powder is steamed in a steaming process. Steaming can be done by the usual method, but be careful not to cook too much. The steaming is for facilitating the next fermentation. However, if the steaming is excessively steamed, the fermentation will proceed excessively, and thus the powder may be slightly moistened.

After the steaming step, the seed koji is seeded on the steamed powder for fermentation. Here, fermentation means decomposing an organic substance by a microorganism, and does not indicate only when a metabolite becomes a simple compound. For example, mild decomposition by koji mold or yeast is also included and is rather preferable. In the fermentation process, the enzymatic action of amylase, protease and the like contained in koji mold promotes the reduction of the molecular weight of proteins and the like, which facilitates the penetration into the living body and enhances the antioxidant action.

In this koji making process, seeding is performed for each type of steamed powder. The seed koji is preferably powdered, and it is preferable to prepare about three kinds and to thoroughly mix different seed koji for each kind of powder. The seeded powder is transferred to a container (ceramic or plastic) and fermented. The aging temperature is 35 to 36 ° C. and it takes at least 3 days to 2 weeks, but the number of days varies depending on the temperature and humidity, so temperature control must be carefully considered. Antioxidants are produced during such aging.

After the koji-making step, each of the koji starting materials after fermentation is immediately dried in a drying step to stop the fermentation. The drying is carried out using an ordinary dryer, but the drying temperature is 100 ° C. or lower. The dried koji raw materials are crushed, and the resulting powders are mixed in the total amount. The pulverization method is not limited, and it is preferable that the powder is a fine powder having a Tyler standard sieve of about 400 mesh. The fine powder also improves the absorption of the components in the body of these living bodies. In this way, the fermented and ground raw material as the first mixture is obtained.

On the other hand, in addition to the fermented and ground raw materials obtained by mixing, a slurry-like ground product obtained by roasting and crushing oil seeds, and a dry ground product of a plant containing vitamin C or a vitamin C derivative. , And are mixed and stirred to prepare a second mixture. Here, examples of the oil seed include sesame, soybean, corn, rapeseed and the like, and sesame is most suitable. This is because sesame contains a lot of vitamin E that suppresses lipid peroxidation. These oil seeds must go through the roasting process described above.

Examples of plants containing vitamin C or a derivative of vitamin C include, but are not limited to, tea leaves, young leaves of radish, juices of lemon and citron, spinach and the like (hereinafter abbreviated as tea leaves). .. Of these, tea leaves are the most preferred, and therefore, daikon radish leaves and yuzu juice are used. This prevents oil seed oxidation. These plants are preferably dried and crushed before use. Especially for tea leaves, it is dried by roasting with a normal heating method to make powder, but Tyler standard sieve 50
It is preferable to use it in the form of fine powder so that the whole amount of it passes through 0 mesh.

As a typical example, the use ratio of each of the oil seed slurry and the crushed plant material containing vitamin C or a vitamin C derivative is 85 parts by weight of the fermentation crushed raw material which is the first mixture. On the other hand, 10 parts by weight of tea leaves,
5 parts by weight of oil seeds, or 5 parts by weight of tea leaves and 5 parts by weight of oil seeds are used for 90 parts by weight of the fermented and pulverized raw material. Next, the above-mentioned mixed solution of tea leaves, sesame seeds, young leaves of radish, and yuzu juice is mixed with the separately prepared first mixture of the above-mentioned fermentation pulverized raw material, and the mixture is sufficiently stirred. The mixture after this mixing step becomes the raw material powder.

Since the raw material powder contains about 12% of water, there is a risk of re-fermentation as it is, and the raw material powder is granulated (in order to improve the transportability in the chemical reaction of the product). granulation) process. Granulation is carried out by using a fluidized granulation dryer while drying in a vacuum. Only then is the plant antioxidant composition completed. The plant antioxidant composition of the present invention has a function of eliminating excess active oxygen generated in the body. Production of active oxygen in excess of the amount required for the defense mechanism of biological tissues causes cerebrovascular disorders, cardiovascular diseases, eye diseases, diabetes, carcinogenesis, etc., and is said to affect aging and inflammation. ing. In other words, various adult diseases and intractable diseases (malignant joint rheumatism, Behcet's disease, Crohn's disease, ulcerative colitis, etc.) as well as burns, trauma, fatigue, sickness,
It is excellent in the prevention and treatment of constipation, etc., and is particularly effective in maintaining health.

[0017]

EXAMPLES The present invention will be described in more detail below with reference to examples, but the present invention is not limited thereto. (Preparation of plant antioxidant composition of this Example) Fresh soybean 30
Parts by weight, 15 parts by weight of wheat germ, 15 parts by weight of pigeon barley and 30 parts by weight of rice bran immediately after rice polishing were individually roasted. That is, each of soybean, wheat germ, pigeon barley and bran was uniformly heated in a constant temperature bath at a temperature of 80 ° C. for 5 hours. Thereafter, these seeds were crushed into powder.

The roasted powders are heated to temperatures of 110 to 12 respectively.
Boiled at 0 ° C. for 30 minutes only. Next, the koji mold was thoroughly mixed with the steamed powder and seeded. The seeded powder was transferred to a ceramic container and fermented at a temperature of 35 to 36 ° C. for only 2 weeks. Immediately after the koji making, the temperature of the koji ingredients was adjusted to 10
The fermentation was stopped by drying at 0 ° C. The dried koji raw material was pulverized into fine powder having a Tyler standard sieve of about 400 mesh.

All of the obtained powders were mixed to obtain a fermented and ground raw material. Separately from the fermented and ground raw material, roasted sesame was roasted and ground to prepare a fluid slurry. Also, dry the tea leaves while roasting, and use Tyler standard sieve 5
A fine powder of about 00 mesh was prepared. A mixed solution of these sesame slurry and tea leaf powder with young radish leaves and yuzu juice was prepared.

This mixed solution was mixed with a separately prepared fermentation pulverized raw material and sufficiently stirred to prepare a raw material powder. The mixing ratio used was 5 parts by weight of tea leaves and 5 parts by weight of tea leaf powder with respect to 90 parts by weight of the fermented and ground material. The raw material powder was granulated to obtain a plant antioxidant composition. The inhibitory effect on lipid peroxidation of the plant antioxidant composition (hereinafter, abbreviated as AOB) obtained as described above was confirmed by animal experiments.

(Experimental method) All male SD rats (Sprague-Dawley rats) (body weight: 200 g-220)
g) was used. 0.5% CM for AOB in in vitro experiments
Used as C suspension. In addition, lipid peroxidation in rat brain homogenate was used as an index by measuring TBA-reactive substances (TBARS).

In the ex vivo experiment, the rats were housed in individual cages, the control group was given only the powdered basic diet, and AO was used.
For group B, mix 1g or 5g of AOB with the basic food,
It was given as a mixed feed. These rats were bred for 1 or 3 days, and then blood cells were collected and used as ESR method samples. DMPO (5,5-dimethyl-1 as a spin trapping agent
-pyrroline-1-oxide) and ESR spectrometer (JEOL-JES
-FR80: manufactured by JEOL Ltd.) was used to examine the superoxide scavenging action in AOB suspension and blood culture using a superoxide generating system of hypoxanthine-xanthine oxidase system.

As a result, in the ESR spin trapping method, AOB showed a strong superoxide scavenging action in both in vitro and ex vivo experiments. The 50% inhibition of superoxide in the AOB suspension was 42 μg / ml. The blood concentration of the control group diluted 12 times was 0.152 ± 0.017 U / ml in terms of SOD activity, but AOB was lg / day.
0.233 ± 0.01U after feeding for 1 day or 3 days with / rat
/ ml, 0.280 ± 0.042 U / ml activity was shown. Similarly 5g / day /
0.233 ± 0.016U / ml, 0.280 ± 0.042U for blood cultured in rats
It showed an activity of / ml. Furthermore, AOB strongly suppressed the lipid peroxidation of rat brain homogenate, and its 50% inhibition rate was 8 μg / ml.

AOB containing flavonoids, tannins, tocopherols, ascorbic acid and the like has a very strong antioxidant action. In particular, its inhibitory effect on lipid peroxidation was stronger than that of α-tocopherol, which has a strong antioxidant effect. It is considered that the strong antioxidant effect of this AOB appears due to the additive synergistic effect of the components contained therein. From these results, it was suggested that AOB has a very strong antioxidant effect.

[0025]

EFFECTS OF THE INVENTION According to the present invention, a plurality of types of plant seeds and germs or bran of plant seeds are independently roasted, then crushed, then steamed, then malted, and then dried powder. The plant antioxidant composition obtained by adding the crushed product of oil seeds and the crushed product of a plant containing vitamin C or a vitamin C derivative to a fermented crushed raw material obtained by mixing In addition, the active oxygen produced excessively in vivo can be effectively suppressed.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁵ Identification code Office reference number FI technical display location A61K 35/78 ADD J 7180-4C // A23L 1/302

Claims (2)

[Claims] 1. A plurality of types of plant seeds and germs or bran of plant seeds are independently roasted, then crushed, then steamed, then koji-made, and then all the dried powders are mixed. A plant antioxidant composition obtained by adding and mixing a crushed product of oil seeds and a crushed product of a plant containing vitamin C or a vitamin C derivative to a fermented crushed raw material and granulating the mixture. 2. The plant seed is selected from soybean, pigeon barley, wheat and rice, the germ is selected from rice or wheat germ and bran, and the oil seed is sesame seed, The plant antioxidant composition according to claim 1, wherein the plant body containing vitamin C and a vitamin C derivative is selected from tea leaves, green leaves and citron.