CN109568202B

CN109568202B - Tea oil essence capable of moistening face

Info

Publication number: CN109568202B
Application number: CN201811534763.9A
Authority: CN
Inventors: 黄立超; 刘卉; 余益
Original assignee: Jiangxi Green Sea Oil Fat Co ltd
Current assignee: Jiangxi Green Sea Oil Fat Co ltd
Priority date: 2018-12-14
Filing date: 2018-12-14
Publication date: 2021-09-21
Anticipated expiration: 2038-12-14
Also published as: CN109568202A

Abstract

The invention provides tea oil essence for moistening face, which mainly comprises oat (AVENA SATIVA) peptide and wild soybean (GLYCINE SOJA) seed extract. The oat (AVENA SATIVA) peptide is prepared by carrying out enzymolysis on oat by acid protease and then carrying out enzymolysis by trypsin; the semen GLYCINEs (Glycine SOJA) seed extract is obtained by defatting semen GLYCINEs (Glycine SOJA) seed with lipase; the skin-moistening and skin-protecting cream has good effects of moistening and protecting the skin, can effectively improve symptoms of dry, rough and wrinkles of the skin, has the effects of softening the skin, clearing DPPH free radicals, preserving moisture, dredging pores, balancing skin grease secretion, providing nutrition for the skin and promoting subcutaneous blood circulation, thereby accelerating skin metabolism, and enabling the skin to be smooth, tender, moist and elastic.

Description

Tea oil essence capable of moistening face

Technical Field

The invention relates to the field of cosmetics, and particularly relates to tea oil essence for moistening the face.

Background

The skin is the largest organ of human body organs and has the functions of protecting, excreting, regulating body temperature, sensing external stimulation and the like. The skin is directly contacted with the external environment, and is easily damaged by various factors such as oxidation, dryness, ultraviolet rays and the like, such as the weather is too dry, body fluids of human bodies are easily damaged, at the moment, if the skin is careless for maintenance, the horny layer has the symptom of water shortage, and the skin becomes dry, rough and even has wrinkles with water shortage. For example, modern life rhythm is busy and dynamic, people need to keep vigorous energy anytime and anywhere due to long-term fatigue and running, sweat and grease are covered on the surface layer of skin thickly, the burden of the skin on the surface is increased, pores are completely blocked, in order to discharge more grease, the pores are gradually large and obvious, the skin is gradually rough, and the skin is unsmooth and uneven.

In order to protect the skin from damage and glow, care must be taken on the daily basis. The skin care comprises deep skin care and surface skin care, and the skin care products have different effects, wherein the main function of the skin care product is to provide nutrition for the skin, and the main function of the skin care product is to add a protective film for the skin to prevent the skin from being damaged by external adverse environment. The skin care is really realized, the skin is firstly softened, so that the cuticle can absorb more water, the penetration and absorption capacity is enhanced, the nutrient substances are contacted with epidermal cells at zero distance and absorbed deeply, and the purposes of activating the skin, smoothing and healthy the skin and filling vitality are achieved.

Modern research finds that oat peptide extracted from oat has a series of skin care effects: 1. moisturizing and softening: the small molecular peptide enters the skin to play a role of a moisturizing factor, can soften the skin, locks the skin moisture and enables the skin to be moisturized; 2. the function of delaying senility: has effects in removing wrinkles, and promoting cell proliferation and metabolism; 3. skin tendering: reducing the roughness of the skin and activating the skin; 4. antioxidation: the function of scavenging free radicals, so that the skin is kept in a young state; 5. inhibition of MMP-1 activity: increasing collagen content in skin, and increasing skin elasticity. The oat peptide can be applied to cosmetics such as moisturizing, anti-wrinkle and aging delaying.

The wild soybean contains precious medicinal components such as soybean isoflavone, soyasaponin, soybean oligosaccharide, soyabean lecithin and the like, and medicinal components such as isoflavone, saponin and the like extracted from the wild soybean have special medicinal effects on human cancer resistance, cardiovascular and cerebrovascular disease treatment, human immunity enhancement, climacteric disease treatment, aging resistance and the like, and have high market development potential.

Disclosure of Invention

Based on the above, the invention aims to provide the tea oil skin-moistening essence with the deep skin-care effect.

The specific technical scheme of the invention is as follows:

the tea oil essence for moistening the face mainly comprises the following components in percentage by mass: 1-5% of camellia seed oil, 5-10% of glycerol, 1-5% of butanediol, 0.1-2% of oat (AVENA SATIVA) peptide, 261-3% of glycerol polyether, 0.1-1% of soluble collagen, 0.05-0.5% of beta-glucan, 0.1-0.5% of acryloyl dimethyl taurate/VP copolymer, 0.01-0.1% of sodium hyaluronate, 0.1-5% of wild soybean (Glycine SOJA) seed extract, 0.01-0.05% of dipotassium glycyrrhizinate, 0.3-0.5% of phenoxyethanol, 0.1-0.3% of methyl hydroxybenzoate, 0.1-0.2% of ethylparaben, 0.05-0.15% of ethylhexyl glycerol, 0.05-0.15% of PEG-50 hydrogenated castor oil, 0.1-3% of nonylphenol polyether-140.01, 0.1-3% of propylene glycol and 70-90% of water.

The invention also provides a preparation method of the oat (AVENA SATIVA) peptide, which comprises the following specific steps:

(1) weighing dried oat, pulverizing, sieving to obtain powder, adding water, homogenizing, adjusting pH to 2.5-3.5, adding acidic protease, and performing enzymolysis;

(2) adjusting the pH value to 7.5-8 after enzymolysis, and adding trypsin for enzymolysis to obtain an enzymolysis solution;

(3) centrifuging the enzymolysis liquid obtained in the step (2), taking supernatant, adding activated carbon for adsorption, and then filtering; ultrafiltering the filtrate with hollow fiber membrane; collecting the permeate to obtain oat peptide.

In some of these embodiments, the sieving of step (1) is through a 10-40 mesh sieve; the amount of the added water is 5-10 times of the weight of the oat powder; the amount of the added acid protease is 0.8-1.5% of the oat by mass, the activity of the protease is 5-10 ten thousand U/g, and the enzymolysis condition is that enzymolysis is carried out for 1-2 hours at 35-40 ℃.

In some of the embodiments, the trypsin is added in the step (2) in an amount of 0.6-1.0% of the oat by mass, and the activity of the trypsin is 5-10 ten thousand U/g; the enzymolysis condition is enzymolysis at 35-40 deg.C for 2.5-3.5 h.

In some embodiments, the centrifugation in step (3) is 4000-; the amount of the added active carbon is 0.3 to 0.5 percent of the mass of the supernatant fluid, and the active carbon is adsorbed for 2 to 4 hours; the hollow fiber membrane is a hollow fiber membrane with the interception relative molecular mass of 3 KD.

The invention also provides a preparation method of the wild soybean (GLYCINE SOJA) seed extract, which comprises the following specific steps:

1) weighing dried semen glycines, pulverizing, and sieving to obtain semen glycines powder; soaking wild soybean seed powder in water, adjusting pH to 5.5-7, adding lipase for enzymolysis to obtain enzymolysis solution;

2) carrying out reflux extraction on the enzymolysis liquid by using an ethanol solution, filtering and collecting filtrate;

3) evaporating the filtrate under reduced pressure, and recovering ethanol to obtain semen GLYCINEs (Glycine SOJA) extract.

In some of these embodiments, the sieving in step 1) is through a 10-40 mesh sieve; the amount of the added water is 3 to 5 times of the weight of the wild soybean seed powder, and the wild soybean seed powder is soaked for 2 to 4 hours.

In some embodiments, the added lipase in step 1) may be porcine pancreatic lipase L3621 or candida lipase LS20, the activity of the lipase in the solution after the addition of the lipase is 1.5-2.5U/ml, and the enzymolysis condition is 35-40 ℃ for 24-36 h.

In some of the examples, the ethanol in step 2) is 95% (V/V), and the reflux extraction conditions are 50-60 ℃ for 2-3 times, each for 1-2 hours.

The invention has the following advantages and beneficial effects:

(1) the tea oil skin-moistening essence is obtained by a large number of experiments and researches of an inventor, and is prepared by selecting tea-oil camellia seed oil, glycerol, oat (AVENA SATIVA) peptide, soluble collagen, beta-glucan, sodium hyaluronate, a wild soybean (GLYCINE SOJA) seed extract and hydrogenated castor oil to be compatible according to a specific proportion, and obtaining an optimal formula composition of the tea-oil skin-moistening essence. The oil tea seed oil has effects of cleaning, diminishing inflammation, protecting and moistening wound surface, softening crust, moistening skin mucosa, and protecting skin; the glycerin and the sodium hyaluronate can improve the hydration degree of the skin cuticle and the water content of the skin; the soluble collagen as an active substance can be diffused to the deep layer of the skin, so that the activity of the collagen in the skin is enhanced, the integrity of the moisture and the fiber structure of the stratum corneum is maintained, the metabolism of skin tissues is promoted, and good moistening, moisturizing, wrinkle removing and beautifying effects are generated on the skin; semen GLYCINEs (Glycine SOJA) extract contains abundant soybean isoflavone, and has effects in promoting fibroblast growth, and activating skin and resisting aging; the small molecular peptide in the oat (AVENA SATIVA) peptide enters the skin to play a role of a moisturizing factor, can soften and remove cutin, lock skin moisture, moisten the skin, and also has the functions of removing wrinkles and accelerating cell proliferation and metabolism.

(2) The oat (AVENA SATIVA) peptide is prepared by carrying out enzymolysis on oat by acid protease and then carrying out enzymolysis by trypsin, the technological parameters of the preparation method of the oat (AVENA SATIVA) peptide are subjected to a large number of experiments and experiences of an inventor to obtain an optimal technological combination, and the oat (AVENA SATIVA) peptide prepared by the method has good small molecular peptide permeability, enters the interior of skin, plays a role of a moisturizing factor, can soften the skin, lock skin moisture and moisten the skin, and also has the effects of removing wrinkles, reducing skin roughness, activating the skin, accelerating cell proliferation and metabolism, keeping the skin in a young state, increasing the collagen content in the skin, increasing the skin elasticity and having a good moisturizing effect.

(3) The wild soybean (Glycine SOJA) seed extract is obtained by extracting wild soybean (Glycine SOJA) seeds after defatting by lipase, and has the functions of activating skin and removing free radicals; contains rich soybean isoflavone and other components, can promote the growth of fiber bud cell, especially has the bidirectional regulating effect of soybean isoflavone on female estrogen, and has good effect of delaying female skin aging.

(4) Practice proves that the tea oil essence can soften skin, remove DPPH free radicals, preserve moisture, dredge pores, balance skin grease secretion, provide nutrition for skin and promote subcutaneous blood circulation, so that skin metabolism is accelerated, aging is delayed, and the skin is smooth, tender, moist and elastic.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in further detail below. However, the embodiments of the present invention are not limited thereto, and the process parameters or conditions not particularly mentioned may be performed by referring to the conventional techniques.

The brief introduction of part of the raw materials involved in the invention is as follows:

the acid protease of the present invention may be "acid protease" available from Henan Ji beauty products Ltd, or "acid protease" available from Zheng Zhou kang Source Industrial products Ltd.

The trypsin of the present invention may be "trypsin" available from Shijiazhuanchunxin Biotechnology Limited or "trypsin" available from Zhengzhou Xuquan Polymer materials Ltd.

The porcine pancreatic lipase L3621 (the enzyme activity is more than or equal to 30000U/g) is purchased from Ruibio company of Germany; the candida lipase LS20 (the enzyme activity is more than or equal to 200000U/g) is purchased from Beijing Kaitai New century biotechnology limited.

The camellia seed oil is derived from mature seeds of camellia oleifera or camellia minutissima of the family Theaceae, and the fatty oil obtained by oil extraction technical methods such as a squeezing method, a leaching method and the like contains rich unsaturated fatty oil, mainly contains unsaturated fatty acid mainly comprising oleic acid and linoleic acid, and also contains vitamin E and antioxidant components, and is recorded in the compendium of materia Medica: the camellia seed oil is cold and cool in nature and sweet and flat in taste, and has the effects of relaxing bowel, clearing heat and eliminating dampness, moistening lung and eliminating phlegm, and benefiting head and eyes. Suitable camellia seed oil can be raw materials provided by own camellia base of green sea oil and fat limited company in Jiangxi province.

The wild soybean of the invention belongs to leguminous and belongs to annual winding herbaceous plants. Wild soybeans are named as follows: green soy bean, epimedium herb, hungry horsebean, faggot bean, wild soybean, physalis pubescens, horsetail bean. Latin learning name: et zucc, the extraction site is a seed. The wild soybean can be extracted with water or ethanol. The wild soybean mainly contains soybean isoflavone, soyasaponin, soybean oligosaccharide, soybean phospholipid and other valuable medicinal components. The cosmetic of the wild soybean seed extract prepared by the invention is named as wild soybean (Glycine SOJA) seed extract.

The oat of the invention is a gramineous avena plant called brome and wild wheat in the compendium of materia medica. Latin is called Avena sativa L, and the oat is not easy to peel, so the oat is called as peel oat, the extraction part is a seed, and the oat can be extracted by using water or alcohol and the like as a solvent according to a conventional method. The main chemical components of oat are oat protein, oat peptide, oat beta glucan, oat oil and the like. The oat peptide prepared by the invention is named as oat (AVENA SATIVA) peptide in the cosmetic.

Example 1

An oat (AVENA SATIVA) peptide is prepared by the following steps:

(1) weighing 1000g of dried oat, crushing, sieving with a 10-mesh sieve to obtain powder, adding 10000g of water, homogenizing, adjusting the pH to 2.5, adding 15g of acid protease, and carrying out enzymolysis at 35 ℃ for 2 hours, wherein the activity of the acid protease is 5 ten thousand U/g;

(2) adjusting the pH value of the enzymolysis liquid to 8 after enzymolysis, adding 6g of trypsin, and carrying out enzymolysis for 3.5h at 35 ℃ to obtain enzymolysis liquid, wherein the activity of the trypsin is 5 ten thousand U/g;

(3) centrifuging the enzymolysis liquid obtained in the step (2) at 4500r/min for 10min, taking supernate, adding active carbon with the mass of 0.3% of that of the supernate, adsorbing for 2 h, and then filtering; ultrafiltering the filtrate with hollow fiber membrane with relative molecular mass of 3 KD; collecting the permeate to obtain oat peptide.

A semen GLYCINEs (Glycine SOJA) extract is prepared by the following steps:

1) weighing 1000g of dried wild soybean seeds, crushing and sieving with a 40-mesh sieve to obtain wild soybean seed powder; adding 3000g of water, soaking for 2 hours, adjusting the pH value to 7, adding 15g of porcine pancreatic lipase L3621, and carrying out enzymolysis at 40 ℃ for 24 hours to obtain an enzymolysis liquid, wherein the activity of the lipase in the solution after the lipase is added is 2.5U/ml;

2) reflux-extracting the enzymolysis solution with 95% (V/V) ethanol solution at 50 deg.C for 3 times, each for 1 hr, filtering, and collecting filtrate;

Example 2

An oat (AVENA SATIVA) peptide is prepared by the following steps:

(1) weighing 1000g of dried oat, crushing, sieving with a 40-mesh sieve to obtain powder, adding 5000g of water, homogenizing, adjusting the pH to 3.5, adding 8g of acid protease, and carrying out enzymolysis at 40 ℃ for 1 hour, wherein the activity of the acid protease is 10 ten thousand U/g;

(2) adjusting the pH value of an enzymolysis solution to 7.5 after enzymolysis, adding 10g of trypsin, and carrying out enzymolysis for 2.5h at 40 ℃ to obtain an enzymolysis solution, wherein the activity of the trypsin is 10 ten thousand U/g;

(3) centrifuging the enzymatic hydrolysate obtained in the step (2) at 4000r/min for 15min, taking the supernatant, adding active carbon with the mass of 0.5% of that of the supernatant for adsorption for 4 hours, and then filtering; ultrafiltering the filtrate with hollow fiber membrane with relative molecular mass of 3 KD; collecting the permeate to obtain oat peptide.

A semen GLYCINEs (Glycine SOJA) extract is prepared by the following steps:

1) weighing 1000g of dried wild soybean seeds, crushing and sieving with a 10-mesh sieve to obtain wild soybean seed powder; adding 5000g of water, soaking for 4 hours, adjusting the pH value to 5.5, adding 20g of candida lipase LS20, and carrying out enzymolysis at 35 ℃ for 36 hours to obtain an enzymolysis liquid, wherein the activity of the lipase in the solution after the lipase is added is 1.5U/ml;

2) reflux-extracting the enzymolysis solution with 95% (V/V) ethanol solution at 60 deg.C for 2 times (each for 2 hr), filtering, and collecting filtrate;

Example 3

An oat (AVENA SATIVA) peptide is prepared by the following steps:

(1) weighing 1000g of dried oat, crushing, sieving with a 20-mesh sieve to obtain powder, adding 7500g of water, homogenizing, adjusting the pH to 3, adding 12g of acid protease, and carrying out enzymolysis at 37 ℃ for 1.5 hours, wherein the activity of the acid protease is 7.5 ten thousand U/g;

(2) adjusting the pH value of an enzymolysis solution to 7.8 after enzymolysis, adding 8g of trypsin, and carrying out enzymolysis for 3h at 38 ℃ to obtain an enzymolysis solution, wherein the activity of the trypsin is 7.5 ten thousand U/g;

(3) centrifuging the enzymatic hydrolysate 4300r/min obtained in the step (2) for 12min, taking the supernatant, adding active carbon with the mass of 0.4% of that of the supernatant for adsorption for 3 hours, and then filtering; ultrafiltering the filtrate with hollow fiber membrane with relative molecular mass of 3 KD; collecting the permeate to obtain oat peptide.

A semen GLYCINEs (Glycine SOJA) extract is prepared by the following steps:

1) weighing 1000g of dried wild soybean seeds, crushing and sieving with a 20-mesh sieve to obtain wild soybean seed powder; adding 4000g of water to soak for 3 hours, adjusting the pH value to 6.5, adding 18g of porcine pancreatic lipase L3621, and performing enzymolysis at 37 ℃ for 30 hours to obtain an enzymolysis liquid, wherein the activity of the lipase in the solution after the lipase is added is 2.0U/ml;

2) reflux-extracting the enzymolysis solution with 95% (V/V) ethanol solution at 55 deg.C for 2 times, each for 1.5 hr, filtering, and collecting filtrate;

Experiment 1

Test for moisture retention

The moisturizing performance of the oat (AVENA SATIVA) peptide samples prepared in examples 1-3 was evaluated experimentally, and a cosmetic liquid containing 20% butylene glycol was used as a blank sample. The test subjects were 48 adult males with dry skin, and were randomly divided into 4 groups of 12 persons each, and the test site was the inner anterior carpal side (20mm × 40mm) of the left and right hands. The external coating method is to coat for 15 days three times a day (morning, noon and evening). The water content of the horny layer was measured in a thermostatic chamber (room temperature 25 ℃ C., humidity 58%) using SKICON200& corneometer under the condition that no external coating sample was present at the external coating site on the last day of external coating (SKICON200 usually only can measure the water content about 20 μm below the skin epidermis; corneometer can measure the water content about 100 μm; the water content from the skin epidermis to the inside can be measured by using both instruments) to confirm the effect of deep water supplement, and the experimental results are shown in Table 1.

Table 1 moisturizing test results table

From the data in the above table, it can be seen that the oat (AVENA SATIVA) peptide samples prepared in examples 1-3 of the present invention have significantly improved skin moisturizing effect after 15 days of continuous use: after the coating is carried out for 30 minutes, the water content is improved by more than 82 percent, and after the coating is carried out for 8 hours, the water content is improved by more than 53 percent; compared with 20% of butanediol beauty lotion, the moisturizing and water locking effects are better.

Experiment 2

DPPH free radical scavenging experiment

Accurately transferring 3.9mL of DPPH solution with the mass concentration of 25.61mg/L, adding 0.1mL of ethanol solution with the volume fraction of 70%, uniformly mixing, and measuring the absorbance Ac at the wavelength of 517 nm. The extract samples of the wild soybean (Glycine SoJA) seeds prepared in examples 1-3 were prepared into different mass concentrations of 2.0, 4.0, and 8.0mg/mL using n-hexane as a solvent, 0.1mL of each extract sample solution of the wild soybean (Glycine SoJA) seeds with different concentrations was accurately transferred, 3.9mL of the PPH solution (mass concentration of 25.61mg/L) was added, mixed well, reacted in the dark at room temperature for 30min, and then the absorbance Ai at wavelength of 517nm was measured. And simultaneously measuring the absorbance Aj of 0.1mL of each sample solution with different concentrations in 3.9mL of ethanol solution with the volume fraction of 70% so as to eliminate the interference of the color of the sample. The results of the experiment are shown in table 2.

DPPH clearance was: DPPH radical scavenging ratio (%) [1- (Ai-Aj)/Ac ]. times.100

TABLE 2 DPPH CLEANING TEST RESULT TABLE

According to the analysis of experimental data, when the concentration of the wild soybean (GLYCINE SOJA) seed extract sample prepared in the examples 1 to 3 is 8.0mg/mL, the DPPH free radical clearance rate of 0.1mL of the sample solution added with 3.9mL of the LDPPH solution (the mass concentration is 25.61mg/L) reaches more than 92%, which shows that the wild soybean (GLYCINE SOJA) seed extract prepared in the examples 1 to 3 of the invention has good DPPH free radical clearance capability, and can be applied to cosmetics for softening and delaying the aging.

Experiment 3 population test

The experimental group 1-3 and the control group 1-3 of the tea oil skin-moistening essence with the compositions shown in the table 3 were prepared, and the experimental group and the control group were composed of the following components in percentage by mass:

TABLE 3 tea oil skin-moistening essence experimental group 1-3 and control group 1-3 component tables

The experimental method comprises the following steps:

1. subject: a total of 180 tested volunteers with dry, rough and wrinkled skin were selected, 67 men and 113 women aged 45 years maximum and 21 years minimum were randomly divided into 6 groups of 30 persons each.

2. Sample preparation: the tea oil skin-moistening essence comprises samples prepared in experimental groups 1-3 and control groups 1-6.

3. The using method comprises the following steps: applying the sample on dry, rough and wrinkle parts of skin once a day, and the dosage is 0.1g/10cm for each time². The using effect is counted after 30 days and 60 days of use.

4. Criteria for therapeutic effect

(1) Remarkably: the symptoms of dry, rough and wrinkle disappear, and the skin becomes smooth, tender, moist and elastic;

(2) the method has the following advantages: eliminating symptoms such as dry skin, roughness, wrinkles and the like;

(3) and (4) invalidation: the symptoms of dry, rough and wrinkled skin are not improved or aggravated.

And recording the data after the test, wherein the days for achieving the obvious effect are less, and the number of people with the obvious effect in the same time is more, so that the medicine effect is better. The results of the trial are shown in Table 4.

TABLE 4 symptom Performance and efficacy profiles of the subjects

5. Analysis of results

(1) Aiming at the symptoms of dry skin, roughness, wrinkles and the like, the experimental group 1-3 tea oil skin moistening essence liquid samples have good skin moistening and protecting effects, and can effectively improve the symptoms of dry skin, roughness, wrinkles and the like.

(2) It is known from the comparison between the experimental group 1-3 and the control group 1-2 that the combination of the extract of the seeds of the wild soybeans (GLYCINE SOJA) and the oat (AVENA SATIVA) peptide has good effects of moistening and caring skin, and compared with the single use of one of the extract of the seeds of the wild soybeans (GLYCINE SOJA) and the oat (AVENA SATIVA) peptide, the combination has better effects of moistening and caring skin for dry, rough and wrinkled skin (the significant effect is 23-26 persons after using the sample of the experimental group 1-3 for 30 days, and the significant effect is 2-4 persons after using the sample of the control group 1-2 for 30 days). After the tea oil skin-moistening essence liquid sample prepared by the experimental groups 1-3 is continuously used for 60 days, the symptoms of the skin dryness, the roughness, the wrinkles and the like of the test user completely disappear, and the skin becomes smooth, tender, moist and elastic.

(3) Compared with the control group 3, the experimental groups 1 to 3 show that when the wild soybean (GLYCINE SOJA) seed extract and the oat (AVENA SATIVA) peptide are absent in the tea oil skin-moistening essence, the skin-moistening effect is not ideal, and after the sample of the control group 3 is used for 60 days, the number of effective fruits is 9, and no case has a remarkable effect.

The revisiting of the subjects using the tea oil skin-moistening essence samples prepared in the experimental groups 1-3 shows that the samples prepared by the method are fresh and pleasant, and have no stimulation to the skin and no toxic or side effect.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention will still fall within the scope of the technical solution of the present invention without departing from the content of the technical solution of the present invention.

Claims

1. The tea oil essence for moistening the face is characterized by comprising the following components in percentage by mass: 1-5% of camellia seed oil, 5-10% of glycerol, 1-5% of butanediol, 0.1-2% of oat (AVENA SATIVA) peptide, 261-3% of glycerol polyether, 0.1-1% of soluble collagen, 0.05-0.5% of beta-glucan, 0.1-0.5% of acryloyl dimethyl taurate/VP copolymer, 0.01-0.1% of sodium hyaluronate, 0.1-5% of wild soybean (Glycine SOJA) seed extract, 0.01-0.05% of dipotassium glycyrrhizinate, 0.3-0.5% of phenoxyethanol, 0.1-0.3% of methyl hydroxybenzoate, 0.1-0.2% of ethylparaben, 0.05-0.15% of ethylhexyl glycerol, 0.05-0.15% of PEG-50 hydrogenated castor oil, 0.1-3% of nonylphenol polyether-140.01, 0.1-3% of propylene glycol and 70-90% of water;

the preparation method of the oat (AVENA SATIVA) peptide comprises the following specific steps: weighing dried oat, crushing, sieving to obtain powder, adding water, homogenizing, adjusting pH to 2.5-3.5, and adding acid protease for enzymolysis; (2) adjusting the pH value to 7.5-8 after enzymolysis in the step (1), and adding trypsin for enzymolysis to obtain an enzymolysis liquid; (3) centrifuging the enzymolysis liquid obtained in the step (2), taking supernate, adding activated carbon for adsorption, and then filtering; ultrafiltering the filtrate with hollow fiber membrane; collecting the permeate to obtain oat peptide;

the preparation method of the wild soybean (Glycine SOJA) seed extract comprises the following specific steps: (I) weighing dried semen glycines, pulverizing, and sieving to obtain semen glycines powder; soaking wild soybean seed powder in water, adjusting pH to 5.5-7, adding lipase for enzymolysis to obtain enzymolysis solution; (II) refluxing and extracting the enzymolysis liquid by using an ethanol solution, filtering and collecting filtrate; (III) evaporating the filtrate under reduced pressure, and recovering ethanol to obtain semen GLYCINEs (Glycine SOJA) extract.

2. The tea oil face-moistening essence according to claim 1, wherein the sieving in step (1) is 10-40 mesh sieving; the amount of the added water is 5-10 times of the weight of the oat powder; the amount of the added acid protease is 0.8-1.5% of the oat by mass, the activity of the protease is 5-10 ten thousand U/g, and the enzymolysis condition is that enzymolysis is carried out for 1-2 hours at 35-40 ℃.

3. The tea oil face-moistening essence according to claim 1, wherein the trypsin is added in the step (2) in an amount of 0.6-1.0% by mass of the oat, and the activity of the trypsin is 5-10 ten thousand U/g; the enzymolysis condition is enzymolysis at 35-40 deg.C for 2.5-3.5 h.

4. The tea oil face-moistening essence liquid as claimed in claim 1, wherein the centrifugation in step (3) is 4000-; the amount of the added active carbon is 0.3 to 0.5 percent of the mass of the supernatant fluid, and the active carbon is adsorbed for 2 to 4 hours; the hollow fiber membrane is a hollow fiber membrane with the interception relative molecular mass of 3 KD.

5. The tea oil face-moistening essence according to claim 1, wherein the sieving in step (I) is a 10-40 mesh sieve; the amount of the added water is 3 to 5 times of the weight of the wild soybean seed powder, and the wild soybean seed powder is soaked for 2 to 4 hours.

6. The tea oil beauty essence according to claim 1, wherein the amount of the added lipase in step (I) is 1.5-2% of the weight of the wild soybean seed powder, the activity of the lipase in the solution after the addition of the lipase is 1.5-2.5U/ml, and the enzymolysis condition is 35-40 ℃ for 24-36 h.

7. The tea oil beauty essence according to claim 1, wherein the ethanol in step (II) is 95% (V/V), and the reflux extraction is performed under 50-60 ℃ for 2-3 times, each for 1-2 hours.