CN102274138A - Dendrobe extract and preparation method and application thereof - Google Patents
Dendrobe extract and preparation method and application thereof Download PDFInfo
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- CN102274138A CN102274138A CN2010101988034A CN201010198803A CN102274138A CN 102274138 A CN102274138 A CN 102274138A CN 2010101988034 A CN2010101988034 A CN 2010101988034A CN 201010198803 A CN201010198803 A CN 201010198803A CN 102274138 A CN102274138 A CN 102274138A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8984—Dendrobium
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
Abstract
The invention discloses a preparation method of a dendrobe extract. The dendrobe extract prepared by adopting the method has the function of preventing keratocyte from dehydrating and can be used for preserving moisture of skin and can also be used as a cosmetic additive. The invention also discloses the application of the dendrobe extract to preparing cosmetics for preserving moisture of the skin.
Description
Technical field
The present invention relates to Natural Medicine Chemistry field and cosmetic field, be specifically related to a kind of Chinese herbal medicine Herba Dendrobii that is used to preserve moisture in the cosmetics.
Background technology
Skin is made of epidermis, corium and subcutaneus adipose tissue, and every layer all contains moisture.Moisture content of skin how much be tender, plentiful, the glossy and whether soft key of skin.Wrinkle dry, flat, the desquamation that withers takes place even because water content is lower so seem in the infant moisture content of skin higher position delicate gloss that seems, old people's skin.Preserving moisture is the essential condition that guarantees skin health, slow down aging, and the wetting agent of developmental research excellent performance is the target that people seek assiduously.
On the one hand with age, skin moisture-keeping mechanism sustains damage, and skin tissue cell and intercellular moisture reduce, and when horny layer moisture drops to 10% when following, that skin will seem is dry, follow the string, wrinkling, acceleration skin aging.Skin suffers infringements such as sweltering heat, cold, wind, cleaning agent, ultraviolet radiation on the other hand, and it is dry more that the top layer can become.Therefore how to keep the moisture of skin, it is very important to reduce moisture loss.Skin moisturizer mainly comprises sealer, hygroscopic agent, hydrophilic substrate, preventing light agent and some special adding ingredients in the market.
Traditional medicine thinks that xerosis cutis is the imbalance of lung renal function, water liquid metabolic imbalance, and the not flourish skin of body fluid, so the road of skin moistening is invigorating the lung and the kidney, nourishing body fluid.The record of skin-moisturizing, skin is also arranged in the Chinese medicine document, and compiled Chinese medicine beauty treatment prescription, all be described from theory into action.Some modern studies are set forth to some extent to the effect of polysaccharide component in recent years, the height water absorption of polysaccharide and good film property are perfectly in conjunction with reaching good moistening effect, vegetable polysaccharides also is applied to skin moisture-keeping just gradually, and the main component that polysaccharide is most Chinese herbal medicine to be contained, all skin moisturizer has indicated direction in order to seek safely and effectively for these.
Summary of the invention
The object of the invention is to provide a kind of preparation method of Herba Dendrobii extract, and described method comprises:
(1) solvent extraction, described solvent are selected from water, methanol, ethanol, isopropyl alcohol, n-butyl alcohol, ethylene glycol, 1,2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination.
Preferably, wherein the extraction of step (1) is selected from immersion, decoction, backflow, percolation or its combination.
Preferably, described preparation method also comprises:
(2) separation and Extraction liquid and residue obtain extracting solution;
(3) optional solvent recovery;
(4) optionally dissolving or dilution.
Preferably, wherein used solvent is selected from water, ethanol, isopropyl alcohol, n-butyl alcohol, ethylene glycol, 1 in the step (4), 2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination.
Another purpose of the present invention is to provide the preparation method of another kind of Herba Dendrobii extract, and described method comprises:
(1) solvent extraction, described solvent are selected from methanol, ethanol, isopropyl alcohol, n-butyl alcohol, ethylene glycol, 1,2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination;
(2) separation and Extraction liquid and residue obtain residue;
(3) residue of water extraction step (2).
Preferably, wherein the extraction of step (1) and (3) is independently selected from immersion, decoction, backflow, percolation or its combination.
Preferably, described preparation method also comprises:
(4) separate aqueous extract and residue, obtain aqueous extract;
(5) concentrate, the ethanol precipitate with ethanol separates obtaining precipitation;
(6) alternatively, the resolution of precipitate with step (5) is mixed with solution.
Preferably, wherein used solvent is selected from ethanol, isopropyl alcohol, n-butyl alcohol, ethylene glycol, 1 in the step (6), 2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination.
Another purpose of the present invention provides the Herba Dendrobii extract according to the preparation method acquisition of the invention described above.
Preferably, the form of described Herba Dendrobii extract is selected from solution, powder, ointment, membrane or cream frost.
The Herba Dendrobii extract that the method according to this invention obtains can be directly used in skin moisture-keeping, also can be used as functional additive and joins in the cosmetics and use.
Further object of the present invention provides the purposes of Herba Dendrobii extract in the cosmetics that preparation is preserved moisture.
Preferably, described cosmetics reveal or essence from beautifying and skin-protecting cream, eye cream, facial film, cleansing milk, toner/smoothing toner, profit body.
Preferably, described Herba Dendrobii extract is the Herba Dendrobii extract of above-mentioned prepared in accordance with the method for the present invention.
Further object of the present invention provides a kind of cosmetics of preserving moisture, and wherein contains Herba Dendrobii extract.
Preferably, described cosmetics beautifying and skin-protecting cream, eye cream, facial film, cleansing milk, toner/smoothing toner, profit body reveal or essence.
Preferably, described Herba Dendrobii extract is the Herba Dendrobii extract of above-mentioned prepared in accordance with the method for the present invention.
In the present invention, Herba Dendrobii (Caulis Dendrobii) is meant fresh or dry this medicinal part of stem of plant Herba Dendrobii, Herba Dendrobii is the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family Dendrobium, mainly comprises the fresh or dry stem of Dendrobium nobile Dendrobium nobile, Herba Dendrobii Dendrobium candidum, Herba Dendrobii oculati Dendrobium fimbriatum var.oculatum, dendrobium loddigesii Rolfe Dendrobium loddigesii, HERBA DENDROBII Dendrobium Chrysanthum and allied species thereof.Mainly be distributed in Yunnan Province of China, Sichuan, Guizhou and torrid areas, Asia.All can take the whole year, to gather autumn to good.Dry or dry, cutting is given birth to and is used.
Herba Dendrobii: sweet cold, return the stomach kidney channel, reinforcing stomach reg fluid, nourishing YIN and clearing away heat, the lung moistening kidney tonifying is used for cloudy impairment of body fluid and loses the xerostomia excessive thirst; Shennong's Herbal: " tonifying five ZANG-organs asthenia weakness and emaciation is made light of one's life by commiting suicide and prolonged life "; " Mingyi Bielu ": " Herba Dendrobii longue meat is by skin pathogenic heat miliaria gas "; " book on Chinese herbal medicine " cloud " the thin person of Herba Dendrobii circle is last, and calls its beneficial smart reinforcing YIN-essence, strong muscle tonify deficiency, and strong foot knee joint drives cold numbness, but palpitation with fear, centering will."; Xu studies carefully core: " clearing stomach of Herba Dendrobii function is promoted the production of body fluid, and the stomach hot person that suffers from a deficiency of the kidney is the most suitable.As desire clearing stomach and rescue Tianjin, agile from non-sweet taste with Herba Dendrobii is not merit.”。Modern pharmacological research shows that the Herba Dendrobii effective ingredient contains compositions such as alkaloid, polysaccharide, trace element and aminoacid, and its extract has outstanding water holding effect, can increase the blood flow of blood capillary; And can significantly improve superoxide dismutase (SOD) level, thereby play the effect that reduces lipid peroxide (LPO); Also from the adjusting angle of brain monoamines neurotransmitter level, as the inhibitor of similar monoamine oxidase, MAO (MAO) and play anti-aging effects.
Herba Dendrobii extract that method of the present invention makes and cosmetics can help skin to resist drying under dry environment, prevent moisture loss, and the skin Caring of preserving moisture makes skin honorable moist, and non-stimulated to skin.
Description of drawings
Figure 1A and Figure 1B are respectively the cellular morphology figure of cell behind the initial and dry 15min of T of blank group;
Fig. 2 A and Fig. 2 B be respectively extract B among the embodiment 2 (1%, the cellular morphology figure of the cell of v/v) hatching behind the initial and dry 15min of T;
Fig. 3 A and Fig. 3 B be respectively extract D among the embodiment 4 (1%, the cellular morphology figure of the cell of v/v) hatching behind the initial and dry 15min of T;
Fig. 4 A and Fig. 4 B be respectively extract E among the embodiment 5 (1%, the cellular morphology figure of the cell of v/v) hatching behind the initial and dry 15min of T.
The specific embodiment
Embodiment 1
Get HERBA DENDROBII Dendrobium Chrysanthum 100g, adding 20 times of amounts (doubly measures: the ratio that refers to solvent volume and Herba Dendrobii weight, wherein the unit of solvent volume is ml, the unit of Herba Dendrobii weight is g, down with) 75% ethanol (v/v) merceration 48 hours, filter, filtrate recycling ethanol, the extract obtained water dissolution of using is made and is contained the solution that the crude drug amount is 1500mg/ml, obtains extracting solution A.
Embodiment 2
Get dendrobium loddigesii Rolfe Dendrobium loddigesii 100g, add 8 times of amount methanol eddies and extracted 2 hours, filter, filtrate is reclaimed methanol, and the extract obtained water dissolution of using is made and contained the solution that the crude drug amount is 1000mg/ml, obtains extracting solution B.
Embodiment 3
Get Herba Dendrobii Dendrobium candidum 100g, add 10 times of amount deionization water boiling and extraction twice, each 1 hour, filter, the extract obtained water dissolution of using is made and is contained the solution that the crude drug amount is 500mg/ml, obtains extracting solution C.
Embodiment 4
Depletion hairpin Herba Dendrobii Dendrobium nobile 100g adds 6 times of amount alcohol reflux, filters, and 10 times of water gagings of residue reuse decoct and extracted about 2 hours, concentrate, add the doubling dose ethanol precipitation, filter, precipitation is used water dissolution, makes to contain the solution that the crude drug amount is 1000mg/ml, obtains extracting solution D.
Embodiment 5
Get Herba Dendrobii oculati Dendrobium fimbriatum var.oculatum 100g, add 8 times of amount ethanol percolate extraction, percolate reclaims ethanol, and the extract obtained water dissolution of using is made and contained the solution that the crude drug amount is 1000mg/ml, obtains extracting solution E.
Embodiment 6 prevents the testing in vitro of horn cell dehydration
According to preserving moisture Related Mechanism, utilize the in-vitro evaluation method of cell platform, under epidermis cell is in dry environment, investigates the embodiment extract and help cell to resist drying, prevent dehydration, keep the ability of the original form of cell.
Get each embodiment extract 0.1ml, add MEM culture fluid 9.9ml, sucking filtration is mixed with 1% concentration.Same method is prepared 1% glycerol as positive control.Selecting people's epidermal keratinocyte Hacat is experimental cell, with normal MEM liquid cultured cells is the blank group, containing the positive matched group of MEM liquid cultured cells of 1% concentration glycerol, be the dosing group with the MEM liquid cultured cells of the embodiment extract that contains 1% concentration.After hatching 24h, blot every hole liquid successively, place the microscopically observation of cell from normally to the time that dry (state significant change) experienced, and in 15min Taking Pictures recording form.
Experimental result:
Under the drying condition that gives in the external world, not adding the blank group cell that the embodiment extract hatches dries out gradually, can resist drying time is 720 seconds, the positive controls that 1% glycerol is hatched is kept with respect to the blank group to be increased drying time to some extent, it is 780 seconds, and the keratinocyte that each embodiment extract is hatched can help Skin Cell to keep moisture, give cell good resist drying ability, can resist drying time is more than 795 seconds, increased more than 10% than blank group, effect surpasses the contrast of 1% glycerol, sees following table for details:
| Average drying is held time (second) | Difference | Percentage ratio | |
| Blank | 720 | ||
| 1% glycerol | 780 | 60 | 8.33％ |
| Embodiment 2 extract B | 795 | 75 | 10.42％ |
| Embodiment 4 extract D | 820 | 100 | 13.89％ |
| Embodiment 5 extract E | 806 | 86 | 11.94％ |
At exsiccant the 15th minute, the serious dehydration of blank group cell shrinkage, index of refraction increases, form obviously changes, and each embodiment extract hatch the group cell keep original form substantially, illustrate that each embodiment extract has good resist drying moisture-keeping function, wherein best with the resist drying moistening effect of embodiment 4 extract D, resisting increases percentage ratio drying time and reaches 13.89%, sees accompanying drawing 1A-4B for details.
Claims (14)
1. the preparation method of a Herba Dendrobii extract comprises:
(1) solvent extraction, described solvent are selected from water, methanol, ethanol, isopropyl alcohol, n-butyl alcohol, ethylene glycol, 1,2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination.
2. preparation method as claimed in claim 1, wherein the extraction of step (1) is selected from immersion, decoction, backflow, percolation or its combination.
3. preparation method as claimed in claim 1 or 2 also comprises:
(2) separation and Extraction liquid and residue obtain extracting solution;
(3) optional solvent recovery;
(4) optionally dissolving or dilution.
4. preparation method as claimed in claim 3, wherein used solvent is selected from water, ethanol, isopropyl alcohol, n-butyl alcohol, ethylene glycol, 1 in the step (4), 2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination.
5. the preparation method of a Herba Dendrobii extract comprises:
(1) solvent extraction, described solvent are selected from methanol, ethanol, isopropyl alcohol, n-butyl alcohol, ethylene glycol, 1,2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination;
(2) separation and Extraction liquid and residue obtain residue;
(3) residue of water extraction step (2).
6. preparation method as claimed in claim 5, wherein the extraction of step (1) and (3) is independently selected from immersion, decoction, backflow, percolation or its combination.
7. as claim 5 or 6 described preparation methoies, also comprise:
(4) separate aqueous extract and residue, obtain aqueous extract;
(5) concentrate, the ethanol precipitate with ethanol separates obtaining precipitation;
(6) alternatively, the resolution of precipitate with step (5) is mixed with solution.
8. preparation method as claimed in claim 7, wherein used solvent is selected from ethanol, isopropyl alcohol, n-butyl alcohol, ethylene glycol, 1 in the step (6), 2-propylene glycol, 1, ammediol, 1,3 butylene glycol, acetone, ethyl acetate or its combination.
9. Herba Dendrobii extract, it makes according to any one described preparation method of claim 1-8.
10. Herba Dendrobii extract as claimed in claim 9, wherein, the form of described Herba Dendrobii extract is selected from solution, powder, ointment, membrane or cream frost.
11. the purposes of Herba Dendrobii extract in the cosmetics that preparation is preserved moisture, described cosmetics are preferably revealed or essence from beautifying and skin-protecting cream, eye cream, facial film, cleansing milk, toner/smoothing toner, profit body.
12. purposes as claimed in claim 11, wherein said Herba Dendrobii extract are claim 9 or 10 described Herba Dendrobii extracts.
13. cosmetics of preserving moisture wherein contain Herba Dendrobii extract, described cosmetics preferably reveal or essence from beautifying and skin-protecting cream, eye cream, facial film, cleansing milk, toner/smoothing toner, profit body.
14. cosmetics as claimed in claim 13, wherein said Herba Dendrobii extract are claim 9 or 10 described Herba Dendrobii extracts.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101988034A CN102274138A (en) | 2010-06-11 | 2010-06-11 | Dendrobe extract and preparation method and application thereof |
| PCT/CN2011/075429 WO2011153937A1 (en) | 2010-06-11 | 2011-06-08 | Herbe dendrobii extract, its preparation method and use thereof |
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| CN2010101988034A CN102274138A (en) | 2010-06-11 | 2010-06-11 | Dendrobe extract and preparation method and application thereof |
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- 2010-06-11 CN CN2010101988034A patent/CN102274138A/en active Pending
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2011
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