CN102258442B - Compound traditional Chinese medicine extract and application thereof to whitening, moisturizing and anti-aging skin care product - Google Patents
Compound traditional Chinese medicine extract and application thereof to whitening, moisturizing and anti-aging skin care product Download PDFInfo
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Abstract
The invention discloses a compound traditional Chinese medicine extract and application thereof to a whitening, moisturizing and anti-aging skin care product. The compound traditional Chinese medicine extract is prepared from the following raw material medicines in parts by mass: 10-50 parts of selaginella, 10-50 parts of peach blossom, 10-50 parts of bletilla and 10-50 parts of pseudo-ginseng. The compound traditional Chinese medicine extract which is prepared from the raw materials has an effect of suppressing tyrosinase and proliferation of melanocyte, has a superior whitening effect, has an effect of proliferating and promoting fibroblast and has an excellent effect of promoting generation of ceramide. The whitening, moisturizing and anti-aging skin care product which is obtained by applying the compound traditional Chinese medicine extract to preparing the whitening, moisturizing and anti-aging skin care product has a durable natural whitening effect and can be used for improving metabolism and moisturizing effects of skin.
Description
Technical field
The invention belongs to the field of Chinese medicines and cosmetics technical field, be specifically related to a kind of herbal mixture extract and the application in whitening skin and preserving moisture defying age skin care item thereof.
Background technology
In recent years, domestic whitening skin and preserving moisture, speckle dispelling, defying age series products have become one of main product in the skin care item.Many chemical toilets, like chemical toilet mercurous, the hydroquinones preparation, this type of cosmetics strong toxicity, potential safety hazard is high.Extensive use now vitamin C, kojic acid and derivant thereof etc. are arranged, but character is unstable, chance light, hot destructible.Chinese medicine is from natural plant amedica; Have different activities compositions such as many active ingredient alkaloids, saponin, flavonoid, organic acid; And different Chinese medicines have different efficacies; What have has a clearing heat, releasing toxin, removing swelling effect, and what have has a function of promoting blood circulation to disperse blood clots, having of having moisten whiten, YIN nourishing dampness effect etc.The pure natural property of Chinese medicine and distinctive effect thereof make itself and extract thereof have unique and significant advantage than synthetic at beautifying face, preserving moisture aspect sun-proof, have characteristics such as nontoxic, harmless, not carcinogenic.
Summary of the invention
Primary and foremost purpose of the present invention is to overcome the defective and the weak point of prior art, and a kind of herbal mixture extract is provided.
Another object of the present invention is to provide the application of described herbal mixture extract.
The object of the invention is realized through following technical proposals: a kind of herbal mixture extract is prepared from following crude drug by mass parts:
Described herbal mixture extract more preferably is prepared from following crude drug by mass parts:
The method for distilling of above-mentioned herbal mixture extract, leaching process are all unqualified.For example can adopt solvent extraction method, solvent is water, ultrasonic extraction, supercritical extraction etc.;
The method for distilling of described herbal mixture extract is more preferably:
(1) with 10～50 mass parts Herba Selaginellaes, 10～50 mass parts Flos persicaes, 10～50 mass parts Pseudobulbus Bletillae (Rhizoma Bletillae)s and 10～50 mass parts notoginseng dryings, crushing screening, obtains coarse powder;
(2) in extraction pot, put into coarse powder and water, leave standstill after the mixing, let medical material fully soak;
(3) heating extraction 60～120min takes out extracting solution, filters, and obtains filtrating;
(4) water is added in the extraction pot, repeating step (3) obtains filtrating again;
(5) filtrating that step (3) and (4) is obtained merges, and concentrates, and is cooled to below 40 ℃; Then, under stirring, add ethanol, make that concentration of alcohol is 60～65% in the mixed liquor that finally obtains;
(6) airtight being placed to precipitated fully;
(7) remove deposition, the supernatant decompression recycling ethanol that obtains obtains concentrated solution;
(8) concentrated solution is filtered, obtain the herbal mixture extract;
Extraction pot described in the step (2) is preferably the TQ-3.0 extraction pot;
The consumption of the water described in the step (2) is preferably 8～12 times that are equivalent to the coarse powder quality, more preferably 10 times by Mass Calculation;
The time of the immersion described in the step (2) is preferably 30min;
The time of heating extraction described in the step (3) is 90min more preferably;
The consumption of the water described in the step (4) is preferably 4～8 times that are equivalent to the coarse powder quality, more preferably 6 times by Mass Calculation;
The time of heating extraction described in the step (4) is 60min more preferably;
The relative density that the concentrated concentrating under reduced pressure that is preferably described in the step (5), spissated degree are preferably filtrating is 1.04～1.06;
Ethanol described in the step (5) is the ethanol of percent by volume 95%;
The time of the airtight placement described in the step (6) is preferably 12h;
Filtration described in the step (8) preferably uses 500 order filter clothes to filter;
The application of described herbal mixture extract in whitening skin and preserving moisture defying age skin care item;
A kind of whitening skin and preserving moisture defying age skin care item contain above-mentioned herbal mixture extract;
The cooperation consumption of above-mentioned herbal mixture extract is 0.05～20% (w/w) in the whitening skin and preserving moisture defying age skin care item of the present invention, and preferable amount is 0.1～5% (w/w);
In whitening skin and preserving moisture defying age skin care item of the present invention, except that above-mentioned essential composition, can suitably cooperate the composition that in cosmetics, uses usually as required.For example, matrix components such as other whitening agent, wetting agent, antioxidant, surfactant, alcohols, water;
The dosage form of whitening skin and preserving moisture defying age skin care item of the present invention is not special to be limited, and can be solution, suspension, emulsion, cream, paste, gel, milk, skin care liquid, powder, fancy soap, oil, dry powder, foundation emulsion, wet-milling or spray.
Principle of the present invention:
Herba Selaginellae is a Herba Selaginellae, and lipoxidase is had inhibitory action.Lipoxidase is the enzyme with the unsaturated fatty acids acid oxidase; And unsaturated fatty acid oil is the main component that constitutes cell membrane; Therefore the inhibition to lipoxidase also is an aspect of skin anti-aging, and Herba Selaginellae extract has the elimination effect to superoxide radical, can be used for the aging resistance of skin; During the Herba Selaginellae extract low concentration melanocyte activity there is powerful inhibition, can be used to remove the cosmetics of skin splash; Its performance of keeping humidity is superior to Aloe.
Flos persicae sweet in the mouth, suffering, slightly warm in nature is gone into the heart, lung, large intestine channel, effects such as the happy skin of invigorating blood circulation, drastic purgation diuresis, blood-activating analgetic.Modern medicine study shows that Flos persicae has beautification function preferably.The beautification function of Flos persicae, mainly be come from contain that kaempferol, perfume (or spice), bean are smart in spending, nutrient substance such as trifolin and vitamin A, B, C.These materials ability blood vessel dilating, promotion of blood circulation, moist skin; Improve blood circulation, promote skin-nourishing and oxygen supply, the plain quickening of the lipofuscin that promotes human senility drained; Prevent melanin chronic deposition in skin, thereby can effectively prevent the aminoacid that also is rich in vegetable protein in chloasma, freckle, black speck, the flowering peach and is free state, easily by skin absorbs; To preventing and treating xerosis cutis, coarse and wrinkle etc. effectively; Also the resistance against diseases of skin be can strengthen, thereby dermatosis, seborrheic dermatitis, suppurative dermatitis, vitamin C deficiency etc. prevented and treated, of great advantage to skin.
Contain abundant raw materials such as starch, glucose, volatile oil and phlegmatic temperament in the Pseudobulbus Bletillae (Rhizoma Bletillae); The function of Pear Power speckle dispelling, astringing to arrest bleeding, detumescence and promoting granulation and effect have the saying of " control skin ulcer on the face, make us flesh sliding " on " property of medicine opinion "; Its " it is black to wash one's face, speckle dispelling " also described on the Compendium of Material Medica.
Research about the Radix Notoginseng cosmetic result in the modern medicine shows: Radix Notoginseng has the effect that fat eliminating is whitened, and this is that the porous skin bottom is removed unnecessary fat boat because it contains the fat eliminating factor, conditioning arteries and veins gas, and blood circulation promoting lets skin whiten naturally; Radix Notoginseng has the effect of wrinkle removal skin care, and this is because it contains the skin care factor, can remove skin blemishes, shines the skin expression, and conditioning arteries and veins gas makes skin more flawless; Radix Notoginseng also has the speckle effect of going in vain only, and this is because it contains the speckle dispelling factor, can to because of the endocrine disturbance or phenomenons such as the sunburn that causes after shining, blackhead, contamination precipitation specially good effect is arranged; Radix Notoginseng has the skin sore of releiving effect, and this is because it contains the factor of invigorating blood circulation of releiving, and can promote the skin blood circulation, strengthens the thin respiratory skin sore regeneration of releiving simultaneously of skin.
The present invention has following advantage and beneficial effect with respect to prior art: herbal mixture extract of the present invention is inhibited to the propagation of tryrosinase and melanocyte, has excellent whitening effect; Have propagation that facilitation is arranged to the fibrous bud cell, meaning to increase skin activity, reduces wrinkle; In epidermis cell was cultivated, above-mentioned herbal mixture extract had good promotion to the generation of brain amide, showed that it can obviously change sebum and form, thereby reduced the oil of skin, improved the soft and moist degree of skin.Because of not containing the chemicals composition, safe, nontoxic, little to skin irritation, stable in properties is applied to have on the skin lasting natural beauty white effect, can improve skin metabolism and moisture preserving function simultaneously.
The specific embodiment
Below in conjunction with embodiment the present invention is described in further detail, but embodiment of the present invention is not limited thereto.
Embodiment 1
(1) Herba Selaginellae, Flos persicae, the Pseudobulbus Bletillae (Rhizoma Bletillae) and Radix Notoginseng were pressed mass ratio 50: 10: 20: 20 proportionings, dry, crushing screening obtain coarse powder;
(2) the 1kg coarse powder is added in the extraction pot (TQ-3.0), add the 10kg drinking water again, soak 30min;
(3) heating extraction is 90 minutes, takes out extracting solution, filters, and obtains filtrating;
(4) add the 6kg drinking water again, heating extraction 60 minutes is filtered, and obtains filtrating;
(5) filtrating that obtains of combining step (3) and (4) is evaporated to and is about 3kg (relative density is 1.06), is cooled to below 40 ℃; Then, under stirring, slowly add the ethanol of percent by volume 95%, make that concentration of alcohol is 63% in the mixed liquor that finally obtains;
(6) airtight placement is 12 hours, makes deposition fully;
(7) get supernatant, decompression recycling ethanol is concentrated into 2kg; Concentrated solution filters with 500 order filter clothes while hot, promptly gets the herbal mixture extract.
Embodiment 2
(1) Herba Selaginellae, Flos persicae, the Pseudobulbus Bletillae (Rhizoma Bletillae) and Radix Notoginseng were pressed mass ratio 15: 40: 10: 35 proportionings, dry, crushing screening obtain coarse powder;
(2) the 1kg coarse powder is added in the extraction pot (TQ-3.0), add the 10kg drinking water again, soak 30min;
(3) heating extraction is 90 minutes, takes out extracting solution, filters, and obtains filtrating;
(4) add the 6kg drinking water again, heating extraction 60 minutes is filtered, and obtains filtrating;
(5) filtrating that obtains of combining step (3) and (4) is evaporated to and is about 3kg (relative density is 1.04), is cooled to below 40 ℃; Then, under stirring, slowly add the ethanol of percent by volume 95%, make that concentration of alcohol is 60% in the mixed liquor that finally obtains;
(6) airtight placement is 12 hours, makes deposition fully;
(7) get supernatant, decompression recycling ethanol is concentrated into 2kg; Concentrated solution filters with 500 order filter clothes while hot, promptly gets the herbal mixture extract.
Embodiment 3
(1) Herba Selaginellae, Flos persicae, the Pseudobulbus Bletillae (Rhizoma Bletillae) and Radix Notoginseng were pressed mass ratio 10: 50: 15: 25 proportionings, dry, crushing screening obtain coarse powder;
(2) the 1kg coarse powder is added in the extraction pot (TQ-3.0), add the 10kg drinking water again, soak 30min;
(3) heating extraction is 90 minutes, takes out extracting solution, filters, and obtains filtrating;
(4) add the 6kg drinking water again, heating extraction 60 minutes is filtered, and obtains filtrating;
(5) filtrating that obtains of combining step (3) and (4) is evaporated to and is about 3kg (relative density is 1.04), is cooled to below 40 ℃; Then, under stirring, slowly add the ethanol of percent by volume 95%, make that concentration of alcohol is 65% in the mixed liquor that finally obtains;
(6) airtight placement is 12 hours, makes deposition fully;
(7) get supernatant, decompression recycling ethanol is concentrated into 2kg; Concentrated solution filters with 500 order filter clothes while hot, promptly gets the herbal mixture extract.
Embodiment 4
(1) Herba Selaginellae, Flos persicae, the Pseudobulbus Bletillae (Rhizoma Bletillae) and Radix Notoginseng were pressed mass ratio 25: 15: 50: 10 proportionings, dry, crushing screening obtain coarse powder;
(2) the 1kg coarse powder is added in the extraction pot (TQ-3.0), add the 10kg drinking water again, soak 30min;
(3) heating extraction is 90 minutes, takes out extracting solution, filters, and obtains filtrating;
(4) add the 6kg drinking water again, heating extraction 60 minutes is filtered, and obtains filtrating;
(5) filtrating that obtains of combining step (3) and (4) is evaporated to and is about 3kg (relative density is 1.05), is cooled to below 40 ℃; Then, under stirring, slowly add the ethanol of percent by volume 95%, make that concentration of alcohol is 64% in the mixed liquor that finally obtains;
(6) airtight placement is 12 hours, makes deposition fully;
(7) get supernatant, decompression recycling ethanol is concentrated into 2kg; Concentrated solution filters with 500 order filter clothes while hot, promptly gets the herbal mixture extract.
Embodiment 5
(1) Herba Selaginellae, Flos persicae, the Pseudobulbus Bletillae (Rhizoma Bletillae) and Radix Notoginseng were pressed mass ratio 30: 10: 10: 50 proportionings, dry, crushing screening obtain coarse powder;
(2) the 1kg coarse powder is added in the extraction pot (TQ-3.0), add the 10kg drinking water again, soak 30min;
(3) heating extraction is 90 minutes, takes out extracting solution, filters, and obtains filtrating;
(4) add the 6kg drinking water again, heating extraction 60 minutes is filtered, and obtains filtrating;
(5) filtrating that obtains of combining step (3) and (4) is evaporated to and is about 3kg (relative density is 1.05), is cooled to below 40 ℃; Then, under stirring, slowly add the ethanol of percent by volume 95%, make that concentration of alcohol is 62% in the mixed liquor that finally obtains;
(6) airtight placement is 12 hours, makes deposition fully;
(7) get supernatant, decompression recycling ethanol is concentrated into 2kg; Concentrated solution filters with 500 order filter clothes while hot, promptly gets the herbal mixture extract.
Effect embodiment:
One, detection method
Use dimethyl sulfoxide respectively the herbal mixture extract of previous embodiment to be mixed with the active ingredient solution of concentration as mass volume ratio 1%, carry out following experiment.
(1) mensuration of free radical scavenging effect
Diphenyl picryl phenylhydrazine (DPPH) solution (solvent of this solution is the to be water) 2ml that gets active ingredient solution 2ml and concentration and be 100 μ mol/L successively adds in the same tool plug test tube, shakes up; Leaving standstill under the room temperature 30 minutes, is blank working sample absorbance under the 517nm wavelength with the solvent.
Suppression ratio: K%=[1-(Ai-Aj)/Ac] * 100%;
The absorbance of Ai:2ml DPPH solution+2ml active ingredient solution;
The absorbance of Aj:2ml active ingredient solution+2ml solvent (being dimethyl sulfoxide);
The absorbance of Ac:2ml DPPH solution+2ml solvent (being dimethyl sulfoxide).
(2) anti-ultraviolet property is measured
In 280～400nm wave-length coverage, every separated 10nm scans, and confirms the light transmittance of active ingredient solution under each wavelength, calculates the average absorption rate of wave-length coverage.
(3) suppress tyrosine vitality test in the mouse melanin cell
The B16 mouse melanin cell (Chinese Academy of Sciences's cell bank) that will be in exponential phase is inoculated in 96 orifice plates, and culture medium is the calf serum of RPMI1640+ percent by volume 10%, every hole 100 μ l (10
4Individual cell/well), put 37 ℃, 5%CO
2After cultivating 24h in the incubator, the active ingredient solution 50 μ l after the interpolation filtration sterilization.After continuing to cultivate 3d, abandoning supernatant, the use pH value is 0.15% (w/v) poly butylene succinate flushing twice of 6.8, every hole adds the PBS (pH value 7.4) that 90 μ l contain 1% (volume fraction) Triton X-100.Ultrasonication in the ice bath, every hole add the L-DOPA (L-dopa) of 10 μ l 10mmol/L, hatch 60min for 37 ℃, the colorimetric in the 475nm place, and each hole absorbance is surveyed in the blank well zeroing.
Suppression ratio=(1-drug group mean light absorbency value+matched group mean light absorbency value) * 100%
(4) melanocyte synthetic test in suppressing in the mouse melanin cell
With the B16 mouse black-in tumor cell with 1 * 10
5The density of individual/ml is inoculated in the culture dish that diameter is 9cm, at 37 ℃, 5%CO
2Condition under cultivated 2 days, abandon supernatant, add the active ingredient solution 18-22ml after the filtration sterilization; Then cultivated 3 days, and abandoned supernatant, the PBS washing; Every hole adds the pancreatin solution peptic cell 3min of 0.5ml mass percent 0.2%, and every ware adds 2ml and keeps liquid termination digestion.Behind the piping and druming mixing, every kind of concentration is taken out 0.5ml and is done the cell counting.All the other cell suspension are abandoned supernatant with the centrifugal 5min of 2500r/min, in deposition, add NaOH solution, and heating makes the melanin dissolving, survey absorbance on the enzyme-linked immunosorbent assay instrument of selection 490nm wavelength.
Melanin synthesizes suppression ratio=[1-(medicine hole absorbance+medicine porocyte density)+(control wells absorbance+control wells cell density)] * 100%
(5) detection of moistening effect:
The test of keratodermatitis hydration rate: operation principle is to be instrument probe with the capacitor; Because water is the maximum material of dielectric constant on the skin; When skin moisture content changes; The capacitance of skin also changes, so can be through measuring skin capacitance, analyzing skin surface moisture content.Instrument commonly used is Corneometer CM825.Weigh the variation of horny layer water content through measuring the variation of using product front and back keratodermatitis capacitance; Thereby this kind of moisture-keeping efficacy method of estimating cosmetics is carried out quantification to the moisture of keratodermatitis; Can react the variation of skin moisture content delicately; And favorable reproducibility is one of present moisture-keeping cosmetics effect evaluation method commonly used.
Experimental technique: forearm is bent the square Experimental Area that side marks 3 * 3cm2 size in the candidate left and right sides; With the test zone of left arm as moisturizer; The corresponding symmetrical region of right arm is a blank; Detect the moisture at each test position then with Corneometer CM825 test, repeat respectively 5 times, draw meansigma methods.With formula (1) calculated hydration rate.
Two, experimental result:
(1) mensuration of free radical scavenging effect, the result is as shown in table 1:
Table 1
| Suppression ratio (%) | |
| Embodiment 1 | 86.4 |
| Embodiment 2 | 88.6 |
| Embodiment 3 | 87.3 |
| Embodiment 4 | 87.8 |
| Embodiment 5 | 88.2 |
(2) anti-ultraviolet property is measured, and the result is as shown in table 2:
Table 2
| Absorbance (%) | |
| Embodiment 1 | 47.2 |
| Embodiment 2 | 51.1 |
| Embodiment 3 | 50.8 |
| Embodiment 4 | 48.0 |
| Embodiment 5 | 49.5 |
(3) suppress tyrosine vitality test in the mouse melanin cell, the result is as shown in table 3:
Table 3
| Suppression ratio (%) | |
| Embodiment 1 | 55.2 |
| Embodiment 2 | 53.9 |
| Embodiment 3 | 53.7 |
| Embodiment 4 | 54.9 |
| Embodiment 5 | 55.2 |
(4) melanocyte synthetic test in suppressing in the mouse melanin cell, the result is as shown in table 4:
Table 4
| Suppression ratio | |
| Embodiment 1 | 58.9 |
| Embodiment 2 | 61.8 |
| Embodiment 3 | 62.0 |
| Embodiment 4 | 59.7 |
| Embodiment 5 | 58.8 |
(5) detection of moistening effect, the result is as shown in table 5:
Table 5
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | |
| 1h | 75％ | 78％ | 77％ | 78％ | 76％ |
| 2h | 58％ | 60％ | 60％ | 60％ | 59％ |
| 4h | 39％ | 40％ | 38％ | 39％ | 38％ |
| 6h | 41％ | 40％ | 37％ | 38％ | 38％ |
| 8h | 40％ | 39％ | 39％ | 38％ | 39％ |
Above-mentioned experimental result shows that effectively active, the inhibition black generation of restraint of tyrosinase of herbal mixture extract of the present invention has the free radical of removing and anti-ultraviolet function simultaneously, and moistening effect is remarkable.
Application implementation example 1
The herbal mixture extract of embodiment 1 preparation is applied in the Herba Selaginellae water moisturiser alive; Wherein, Herbal mixture extract 1 mass parts, glyceryl stearate 6 mass parts, triglyceride 5 mass parts, polydimethylsiloxane 4 mass parts, cetearyl alcohol 3 mass parts, glycerol 2 mass parts, carbomer 0.5 mass parts, pantothenylol 5 mass parts, triethanolamine 0.5 mass parts, EDTA disodium 0.1 mass parts, hyaluronate sodium 0.1 mass parts and disodium glycyrrhizinate 0.1 mass parts; Daily essence 0.1 mass parts, DMDM Hydantoin 0.2 mass parts, water is supplemented to 100 mass parts.Wherein: glyceryl stearate, triglyceride, polydimethylsiloxane and cetearyl alcohol are the A component; Water, glycerol, carbomer, pantothenylol and EDTA disodium are the B component; Triethanolamine is the C component; Hyaluronate sodium, disodium glycyrrhizinate, daily essence and DMDM Hydantoin are the D component.The herbal mixture extract is dissolved in the B component, is heated to 85 ℃ respectively with the A component then; The C component is added the B component, stir, the A component is added in aforementioned B component that obtains and the C component mixed phase again, behind the homogenizing, be cooled to 45 ℃ and add the D component, 36 ℃ stop to stir discharging, obtain Herba Selaginellae water moisturiser alive.
The Herba Selaginellae water for preparing moisturiser alive is carried out clinic trial to be observed.
The object of observation: 20～45 years old age middle-aged women.Choosing is facial to have middle aged women 100 examples of pigments such as in various degree chloasma, butterfly spot, senile plaque.
Observational technique: behind the cleaning skin, coat above-mentioned Herba Selaginellae water moisturiser alive before sleep every night.Using 28 days continuously is a course of treatment.
Criterion of therapeutical effect: (1) produce effects: melanin disappears and reaches more than 90%, and skin has significantly moist smooth feeling; (2) effective: melanin disappears more than 50%, and skin is moist smooth; (3) invalid: no change before and after the treatment.The result is as shown in table 6:
Table 6
Application implementation example 2
The herbal mixture extract of embodiment 2 preparations is applied in the Herba Selaginellae water moisturiser alive; Wherein, Herbal mixture extract 1 mass parts, glyceryl stearate 6 mass parts, triglyceride 5 mass parts, polydimethylsiloxane 4 mass parts, cetearyl alcohol 3 mass parts, glycerol 2 mass parts, carbomer 0.5 mass parts, pantothenylol 5 mass parts, triethanolamine 0.5 mass parts, EDTA disodium 0.1 mass parts, hyaluronate sodium 0.1 mass parts and disodium glycyrrhizinate 0.1 mass parts; Daily essence 0.1 mass parts, DMDM Hydantoin 0.2 mass parts, water is supplemented to 100 mass parts.Wherein: glyceryl stearate, triglyceride, polydimethylsiloxane and cetearyl alcohol are the A component; Water, glycerol, carbomer, pantothenylol and EDTA disodium are the B component; Triethanolamine is the C component; Hyaluronate sodium, disodium glycyrrhizinate, daily essence and DMDM Hydantoin are the D component.The herbal mixture extract is dissolved in the B component, is heated to 85 ℃ respectively with the A component then; The C component is added the B component, stir, the A component is added in aforementioned B component that obtains and the C component mixed phase again, behind the homogenizing, be cooled to 45 ℃ and add the D component, 36 ℃ stop to stir discharging, obtain Herba Selaginellae water moisturiser alive.
The Herba Selaginellae water for preparing moisturiser alive is carried out clinic trial to be observed.
The object of observation: 20～45 years old age middle-aged women.Choosing is facial to have middle aged women 100 examples of pigments such as in various degree chloasma, butterfly spot, senile plaque.
Observational technique: behind the cleaning skin, coat above-mentioned Herba Selaginellae water moisturiser alive before sleep every night.Using 28 days continuously is a course of treatment.
Criterion of therapeutical effect: (1) produce effects: melanin disappears and reaches more than 90%, and skin has significantly moist smooth feeling; (2) effective: melanin disappears more than 50%, and skin is moist smooth; (3) invalid: no change before and after the treatment.The result is as shown in table 7:
Table 7
The foregoing description is a preferred implementation of the present invention; But embodiment of the present invention is not restricted to the described embodiments; Other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; All should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (7)
1. herbal mixture extract is characterized in that being prepared from following crude drug by mass parts:
10～50 parts of Herba Selaginellaes
10～50 parts in Flos persicae
10～50 parts of the Pseudobulbus Bletillae (Rhizoma Bletillae)s
10～50 parts of Radix Notoginseng;
Concrete steps are:
(1) with 10～50 mass parts Herba Selaginellaes, 10～50 mass parts Flos persicaes, 10～50 mass parts Pseudobulbus Bletillae (Rhizoma Bletillae)s and 10～50 mass parts notoginseng dryings, crushing screening, obtains coarse powder;
(2) in extraction pot, put into coarse powder and water, leave standstill after the mixing, let medical material fully soak;
(3) heating extraction 60～120min takes out extracting solution, filters, and obtains filtrating;
(4) water is added in the extraction pot, heating extraction 60～120min takes out extracting solution, filters, and obtains filtrating again;
(5) filtrating that step (3) and (4) is obtained merges, and concentrates, and is cooled to below 40 ℃; Then, under stirring, add ethanol, make that concentration of alcohol is 60～65% in the mixed liquor that finally obtains;
(6) airtight being placed to precipitated fully;
(7) remove deposition, the supernatant decompression recycling ethanol that obtains obtains concentrated solution;
(8) concentrated solution is filtered, obtain the herbal mixture extract.
2. herbal mixture extract according to claim 1 is characterized in that being prepared from following crude drug by mass parts:
15～50 parts of Herba Selaginellaes
10～40 parts in Flos persicae
10～20 parts of the Pseudobulbus Bletillae (Rhizoma Bletillae)s
20～35 parts of Radix Notoginseng.
3. the described herbal mixture preparation method of extract of claim 1 is characterized in that comprising following steps:
(1) with 10～50 mass parts Herba Selaginellaes, 10～50 mass parts Flos persicaes, 10～50 mass parts Pseudobulbus Bletillae (Rhizoma Bletillae)s and 10～50 mass parts notoginseng dryings, crushing screening, obtains coarse powder;
(2) in extraction pot, put into coarse powder and water, leave standstill after the mixing, let medical material fully soak;
(3) heating extraction 60～120min takes out extracting solution, filters, and obtains filtrating;
(4) water is added in the extraction pot, heating extraction 60～120min takes out extracting solution, filters, and obtains filtrating again;
(5) filtrating that step (3) and (4) is obtained merges, and concentrates, and is cooled to below 40 ℃; Then, under stirring, add ethanol, make that concentration of alcohol is 60～65% in the mixed liquor that finally obtains;
(6) airtight being placed to precipitated fully;
(7) remove deposition, the supernatant decompression recycling ethanol that obtains obtains concentrated solution;
(8) concentrated solution is filtered, obtain the herbal mixture extract.
4. herbal mixture preparation method of extract according to claim 3 is characterized in that:
Extraction pot described in the step (2) is the TQ-3.0 extraction pot;
The consumption of the water described in the step (2) is by Mass Calculation, for being equivalent to 8～12 times of coarse powder quality;
The time of the immersion described in the step (2) is 30min;
The time of heating extraction described in the step (3) is 90min;
Ethanol described in the step (5) is the ethanol of percent by volume 95%;
The consumption of the water described in the step (4) is by Mass Calculation, for being equivalent to 4～8 times of coarse powder quality;
The time of heating extraction described in the step (4) is 60min;
Simmer down to concentrating under reduced pressure described in the step (5);
The time of the airtight placement described in the step (6) is 12h;
Being filtered into described in the step (8) uses 500 order filter clothes to filter.
5. claim 1 or the 2 described herbal mixture extracts application in preparation whitening skin and preserving moisture defying age skin care item.
6. the application of herbal mixture extract according to claim 5 in preparation whitening skin and preserving moisture defying age skin care item, it is characterized in that: the concentration of described herbal mixture extract in whitening skin and preserving moisture defying age skin care item is mass percent 0.05～20%.
7. the application of herbal mixture extract according to claim 6 in preparation whitening skin and preserving moisture defying age skin care item, it is characterized in that: the concentration of described herbal mixture extract in whitening skin and preserving moisture defying age skin care item is mass percent 0.1～5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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| CN102525852B (en) * | 2011-12-23 | 2013-06-05 | 广州市娇兰化妆品有限公司 | Natural plant extract with sunscreen function and preparation method and application thereof |
| CN102793642B (en) * | 2012-08-28 | 2014-01-15 | 广州丹奇日用化工厂有限公司 | Skin care product composition |
| CN103622866B (en) * | 2013-06-30 | 2015-03-04 | 金玛瑙香水（明光）有限公司 | Perfume capable of whitening and preparation method thereof |
| CN103349637B (en) * | 2013-07-31 | 2014-12-24 | 广州市娇兰化妆品有限公司 | Compound traditional Chinese medicine extract with effects of whitening and moisturizing, preparation method and application thereof |
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| CN1099971A (en) * | 1993-09-10 | 1995-03-15 | 许都君 | Plant cosmetic powder |
| CN101366690B (en) * | 2008-09-28 | 2010-11-10 | 李静 | Composition for face pack for removing speckle and wrinkle, and preparation method thereof |
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