CN103349637B - Compound traditional Chinese medicine extract with effects of whitening and moisturizing, preparation method and application thereof - Google Patents
Compound traditional Chinese medicine extract with effects of whitening and moisturizing, preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the field of traditional Chinese medicines, and discloses a compound traditional Chinese medicine extract with effects of whitening and moisturizing, a preparation method and an application thereof. The compound traditional Chinese medicine extract is prepared from the following traditional Chinese medicine raw materials, by mass: 10-50 parts of common bletilla tuber, 10-50 parts of szechwan lovage rhizome, 10-50 parts of oriental waterplantain tuber, 10-35 parts of heartleaf houttuynia herb, 10-35 parts of glossy privet fruit, and 10-35 parts of nutgrass galingale rhizome. According to the present invention, a supercritical extraction technology and ZLUPD high pressure difference continuous low-temperature efficient extraction are adopted to extract active components of the Chinese herbals, and advantages of high quality controllability, high extraction efficiency, high utilization rate, low running cost, low environmental pollution and the like are provided.
Description
Technical field
The invention belongs to the field of Chinese medicines, particularly a kind of have Chinese medicinal compound extract of whitening skin and preserving moisture effect and preparation method thereof and application.
Background technology
A lot of whitening skin and preserving moisture product many employings synthetic is to take stopgap measures on the market at present, though effective in cure fast advantage, toxic and side effects is obvious, and curative effect is very unstable.Chinese medicine whitening skin and preserving moisture is a very traditional natural method, and exquisite is, and conditioning is main, is improved the quality of skin, safety non-toxic, stable curative effect by the metabolism of regulation of skin and microcirculation.
Summary of the invention
In order to solve above-mentioned the deficiencies in the prior art part, primary and foremost purpose of the present invention is to provide a kind of Chinese medicinal compound extract with whitening skin and preserving moisture effect.
Another object of the present invention is to provide the above-mentioned preparation method with the Chinese medicinal compound extract of whitening skin and preserving moisture effect.
Another object of the present invention is to provide the above-mentioned application with the Chinese medicinal compound extract of whitening skin and preserving moisture effect.
Object of the present invention is achieved through the following technical solutions: a kind of Chinese medicinal compound extract with whitening skin and preserving moisture effect, and this Chinese medicinal compound extract is prepared from by following raw material of Chinese medicine according to the mass fraction:
Pseudobulbus Bletillae (Rhizoma Bletillae) 10 ~ 50 parts
Rhizoma Chuanxiong 10 ~ 50 parts
Rhizoma Alismatis 10 ~ 50 parts
Herba Houttuyniae 10 ~ 35 parts
Fructus Ligustri Lucidi 10 ~ 35 parts
Rhizoma Cyperi 10 ~ 35 parts.
Preferably, this Chinese medicinal compound extract is prepared from by following raw material of Chinese medicine according to the mass fraction:
Pseudobulbus Bletillae (Rhizoma Bletillae) 35 ~ 45 parts
Rhizoma Chuanxiong 35 ~ 45 parts
Rhizoma Alismatis 20 ~ 30 parts
Herba Houttuyniae 15 ~ 25 parts
Fructus Ligustri Lucidi 10 ~ 20 parts
Rhizoma Cyperi 10 ~ 20 parts.
The above-mentioned preparation method with the Chinese medicinal compound extract of whitening skin and preserving moisture effect, comprises following operating procedure:
(1) by 10 ~ 50 mass parts Rhizoma Chuanxiongs, 10 ~ 35 mass parts Herba Houttuyniae and 10 ~ 35 mass parts Rhizoma Cyperis respectively dry, pulverize and sieve, obtain coarse powder; Gained coarse powder is adopted CO
2supercritical fluid extraction, separated and collected volatile effective component and fat-soluble effective ingredient; Described CO
2the extraction temperature of supercritical fluid extraction 35 ~ 55 DEG C, extracting pressure 20 ~ 50Mpa, extraction time is 1 ~ 3h, CO
2flow velocity is 10 ~ 25L/h; Described separation adopts temperature 35 ~ 55 DEG C, and pressure is 3 ~ 10Mpa;
(2) by 10 ~ 50 mass parts Pseudobulbus Bletillae (Rhizoma Bletillae), 10 ~ 50 mass parts Rhizoma Alismatis and 10 ~ 35 mass parts Fructus Ligustri Lucidi respectively dry, pulverize and sieve, obtain coarse powder; By gained coarse powder and step (1) through CO
2medicinal residues after supercritical fluid extraction are placed in pressure reduction extractor; Described pressure reduction extractor adopts condensed water temperature at 2 ~ 7 DEG C;
(3) evacuation: in 20 ~ 50 seconds, pressure reduction extractor is evacuated to-50MPa ~-100MPa, keep 2 ~ 5 minutes, the aqueous solution spraying into 15 DEG C extracts;
(4) vacuum is subtracted: pass into the compressed air through purification, in 1 ~ 3 minute, make pressure in pressure reduction extractor rise to 0MPa;
(5) pressurize: continue to pass into the compressed air through purification, make the pressure in pressure reduction extractor reach 3MPa, keep 2 ~ 5 minutes;
(6) reduce pressure: stop pressurization, evacuation makes pressure in pressure reduction extractor be down to 0Mpa again;
(7) by step (3) ~ (6) cycling;
(8) be separated, concentrated: to carry out solid-liquid separation by centrifugal, centrifugal liquid is filtered, and filtrate concentrates, and obtains concentrated solution;
(9) step (2) gained volatile effective component and fat-soluble effective ingredient are mixed homogeneously with step (8) gained concentrated solution, obtain the compound Chinese medicine extract for whitening skin and preserving moisture.
The consumption of step (3) described aqueous solution is 5 times of raw material of Chinese medicine quality.
The number of times of step (7) described circulation is 5 ~ 7 times; Step (8) described filtration adopts to be filtered by hollow fiber filter, and described concentrated solution is 1.02 the relative density of 25 DEG C.
Above-mentioned a kind of Chinese medicinal compound extract with whitening skin and preserving moisture effect is preparing the application in whitening skin and preserving moisture skin care item.
The described mass percentage of Chinese medicinal compound extract in whitening skin and preserving moisture skin care item with whitening skin and preserving moisture effect is 0.1 ~ 20%, and preferred mass percentage composition is 1 ~ 8%.
Whitening agent, wetting agent, antioxidant, surfactant, alcohol compound or water can also be contained in described whitening skin and preserving moisture skin care item.
Described whitening skin and preserving moisture skin care item are solution, suspension, emulsion, cream, paste, gel, milk, skin care liquid, powder, fancy soap, oil, dry powder, foundation emulsion, wet-milling or spray.
The pharmacologic effect of various raw material of Chinese medicine is as follows:
Pseudobulbus Bletillae (Rhizoma Bletillae) in compound Chinese medicine extract of the present invention is the dry tuber of orchid Pseudobulbus Bletillae (Rhizoma Bletillae).Be rich in the number of chemical composition such as Pseudobulbus Bletillae (Rhizoma Bletillae) colloid, starch, glucose, volatile oil, phlegmatic temperament and anthraquinone derivative, blestrine B, have effect of whitening and speckle dispelling, astringing to arrest bleeding, detumescence and promoting granulation, wound hemorrhage, carbuncle, scald, furuncle etc. are controlled in external application; External is embrocated, and can eliminate the vestige under on the face as acne, active balance skin, keeps skin water profit.Be used for Head And Face beauty therapeutic and aesthetic health care, epithelial cell reparation can be promoted, participate in reparation and the metabolic process of damaged tissues and cell directly.Especially contained Pseudobulbus Bletillae (Rhizoma Bletillae) colloid and Pseudobulbus Bletillae (Rhizoma Bletillae) glucosan are considered to the stimulus object non-undetermined in immune system for a long time; usually macrophage activation can be caused; and macrophage activation can cause a series of reaction; first fibroblast and Keratinocytic migration can be caused; thus generation epithelical cell growth factor (ECGF) and angiogenesis factor (AF) carry out the skin wrinkling to defying age; promote the generation of collagen protein and elastin laminin simultaneously, thus improve skin appearance, smooth away wrinkles.
The pungent temperature of Rhizoma Chuanxiong is fragrant dry, has blood-activating and qi-promoting, the effect of wind-expelling pain-stopping.Main chemical composition has ligustilide, ligustrazine, ferulic acid, folic acid, vitamin A etc., is usually used in department of dermatologry illness.The object of activating skin cells and delay skin aging is reached by improving cutaneous circulation and suppress the normal thing of Senescence Metabolism in skin tissue cell, vitamin A contained by it has certain nourishing effect, also have vitamin antagonist E to lack and suppress the effect of TYR enzyme, skin of face is brightened, lubricates gloss, the generation of acne and various pigmented spots can be prevented.The ferulic acid contained in Rhizoma Chuanxiong is the cosmetic factor that scientific circles generally acknowledge, it can prevent the mottle that formed because of Exposure to Sunlight and freckle, can also suppress the excessive generation of free radical, improve skin quality, endocrine regulation, makes the fine and smooth gloss of skin, high resilience.Its mechanism of action is by suppressing chromatophorous tyrosine Enzyme (Tyrosinase) and copper ion to react, reducing melanic formation and precipitation.
Rhizoma Alismatis sweet in the mouth, light, cold, can promoting diuresis to eliminate damp pathogen, clearing away damp-heat, damp face of preserving youthful looks.Main component fatty acids, aminoacid, ovum lipoid, saccharide and various trace elements, can nutrition and moist skin, and make skin smooth tender, wrinkle is unfolded.Also containing Alisol A, B, C and acetas etc., have and strengthen immunity, antiinflammatory, antiviral, antibacterial effect, the blood circulation of cortex can be accelerated, have check melanin formation effect, there is effect that is soft and smooth, moist and conditioning skin.Modern department of dermatologry is usually used in treating various dermatopathy, has obvious curative effect to eczema, struvite and coarse skin.
Herba Houttuyniae is a kind of perennial herbaceous plant, energy heat-clearing and toxic substances removing; Removing pus and relieving carbuncle.Herb is containing volatile oil, including antibiotic effective ingredient is decanoylacetaldehyde (i.e. houttuynine sodium bisulfite), lauryl aldehyde, a-pinene and linalool etc., there is antibacterial, antiviral effect, the flavone compounds such as contained Quercetin, quercitrin and Isoquercitrin also have remarkable antiinflammatory action, and the energy blood capillary that significantly inflammation-inhibiting is early stage is hyperfunction.Also there is the effect of radiation resistance and enhancing human body immunity function simultaneously, and without any side effects,
Fructus Ligustri Lucidi is the dry mature fruit of oleaceae plant, and sweet in the mouth, hardship are cool in nature, returns Liver and kidney two warp.There is nourishing the liver and kidney, merit that tonifying YIN nourishes blood.Oleanolic acid, ursolic acid, mannitol, phospholipid and saccharide is contained as Fructus Ligustri Lucidi polysaccharide, arabinose, glucose etc. in Fructus Ligustri Lucidi, also containing 15 seed amino acids, 11 kinds of trace element and volatilization wet goods.Modern medicine study proves that Fructus Ligustri Lucidi has blood sugar lowering and blood fat reducing and has study of anti-atherogenic effect, antibacterial, antivirus action in addition, Fructus Ligustri Lucidi decoct all has inhibitory action to staphylococcus aureus, shigella flexneri, Bacillus typhi, bacillus pyocyaneus and escherichia coli, and there is significant immunological enhancement, scavenging free radicals thus defying age.
Rhizoma Cyperi, mainly containing volatile oil composition, in addition, also has saccharide, alkaloid, glycoside, flavonoid, phenols and triterpenoid compound etc.Containing multiple monoterpene, sesquiterpene and oxide thereof in Rhizoma Cyperi volatile oil.Modern pharmacology experiment shows, Rhizoma Cyperi volatile oil has slight estrogenic activity, can make the complete keratinization of epithelial cell, in volatile oil component, the strongest with the effect of Rhizoma Cyperi alkene.The flavones ingredient contained in Rhizoma Cyperi is a kind of effective free radical scavenger, has antioxidation, also fairly obvious to the functions of removing chloasma effect of human body.
Principle of the present invention is:
Compound Chinese medicine extract of the present invention utilizes ZLUPD High Pressure Difference continuous low-temperature high-efficient extraction technology to extract to be prepared from.When extracting under employing High Pressure Difference particularly cryogenic conditions, effectively can prevent the change of medical material complicated ingredient, extracting solution carries out physical separation by Using Multistage Membranes and concentrates, effectively can remove the insoluble part of solvent, effectively can intercept and can cause the impurity such as macromolecular substances, dust such as the tannin of Skin Cell clogging of pores.The method of High Pressure Difference extract at low temperature not only increases the utilization rate of effective ingredient, and most solvent reusable edible, and quality controllable degree is high, and power consumption is few.
CO
2supercritical fluid extraction (Supeporitical Fluid Extraction, SFE) technology is the new technique that developed recently gets up.Fluid has the dual characteristics of gas-liquid two-phase concurrently in the supercritical state, has both had the high diffusivity coefficient suitable with gas and low viscosity, has again the density close with liquid and the solvability good to material, therefore can be used as solvent and extract.To the extraction of traditional medicine volatile oil, traditional method is steam distillation, but it has many drawbacks, and as effective ingredient is easily destroyed, extraction time is long.SFE technology compare with it its major advantage just like: volatile oil yield obviously, extraction time shortens, not have the organic solvent etc. that remains.
The present invention has following advantage and beneficial effect relative to prior art: the present invention uses supercritical extraction technology and ZLUPD High Pressure Difference continuous low-temperature high efficiency extraction to extract the effective ingredient in Chinese herbal medicine, has that quality controllability is high, extraction efficiency is high, utilization rate is high, operating cost is low and the advantage such as environmental pollution is low.Compound Chinese medicine extract of the present invention, modern pharmacological research finds that these medicines can enhance SOD activity, scavenging free radicals, anti peroxidation of lipid, reduces plasma fibrinogen concentration, promotes cell depolymerization, reduce blood viscosity, blood vessel dilating, improves blood flow state, endocrine regulation, increases facial nutrition, effectively can remove aging cutin, improve the metabolism of skin, increase skin activity, blood circulation promoting, reduce wrinkle; Improve the soft and moist degree of skin.The effect of the defying age had, whitening, moisturizing; Simultaneously because of the plant component of pure natural, safe, nontoxic, little to skin irritation, stable in properties.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
(1) by 10 mass parts Rhizoma Chuanxiongs, 35 mass parts Herba Houttuyniae and 35 mass parts Rhizoma Cyperis respectively dry, pulverize and sieve, obtain coarse powder; Gained coarse powder is put into extraction kettle, adopts CO
2supercritical fluid extraction, separated and collected volatile effective component and fat-soluble effective ingredient; Described CO
2the extraction temperature of supercritical fluid extraction 45 DEG C, extracting pressure 35Mpa, extraction time is 150min, CO
2flow velocity is 20L/h, and described separation adopts temperature 45 C, and pressure is 7Mpa;
(2) by 10 mass parts Pseudobulbus Bletillae (Rhizoma Bletillae), 10 mass parts Rhizoma Alismatis and 35 mass parts Fructus Ligustri Lucidi respectively dry, pulverize and sieve, obtain coarse powder; By gained coarse powder and step (1) through CO
2medicinal residues after supercritical fluid extraction are placed in pressure reduction extractor; Described pressure reduction extractor adopts condensed water temperature at 4 DEG C;
(3) evacuation: in 30 seconds, utilizes vacuum station evacuation, makes to form vacuum in pressure reduction extractor, vacuum degree control is at-50MPa ~-100MPa, keep 3 minutes, spray into the aqueous solution of 15 DEG C, its quality be equivalent to step (1) and (2) use 5 times of raw material of Chinese medicine quality;
(4) vacuum is subtracted: pass into the compressed air through purification, make pressure in pressure reduction extractor rise to 0Mpa gradually, take 2 minutes;
(5) pressurize: continue to pass into the compressed air through purification, make the pressure in pressure reduction extractor reach 3MPa, keep 3 minutes; ;
(6) reduce pressure: stop pressurization, start evacuation, make pressure in pressure reduction extractor be down to 0Mpa;
(7) circulation is extracted: continue step (3) ~ (6), circulate 6 times;
(8) be separated, concentrated: carry out solid-liquid separation by centrifugal, the hollow fiber filter that centrifugal liquid is 1.0 μm by aperture is filtered to clarification; Filtrate concentrated, being concentrated into relative density is 1.02(25 DEG C of measurement, with the density of water for benchmark);
(9) step (2) gained volatile effective component and fat-soluble effective ingredient are mixed homogeneously with step (8) gained concentrated solution, obtain the compound Chinese medicine extract I for whitening skin and preserving moisture.
Embodiment 2
(1) by 35 mass parts Rhizoma Chuanxiongs, 15 mass parts Herba Houttuyniae and 10 mass parts Rhizoma Cyperis respectively dry, pulverize and sieve, obtain coarse powder; Gained coarse powder is put into extraction kettle, adopts CO
2supercritical fluid extraction, separated and collected volatile effective component and fat-soluble effective ingredient; Described CO
2the extraction temperature of supercritical fluid extraction 45 DEG C, extracting pressure 35Mpa, extraction time is 150min, CO
2flow velocity is 20L/h; Described separation adopts temperature 45 C, and pressure is 7Mpa;
(2) by 35 mass parts Pseudobulbus Bletillae (Rhizoma Bletillae), 20 mass parts Rhizoma Alismatis and 10 mass parts Fructus Ligustri Lucidi respectively dry, pulverize and sieve, obtain coarse powder; By gained coarse powder and step (1) through CO
2medicinal residues after supercritical fluid extraction are placed in pressure reduction extractor; Described pressure reduction extractor adopts condensed water temperature at 4 DEG C;
(3) evacuation: in 30 seconds, utilizes vacuum station evacuation, makes to form vacuum in pressure reduction extractor, vacuum degree control is at-50MPa ~-100MPa, keep 3 minutes, spray into the aqueous solution of 15 DEG C, its quality be equivalent to step (1) and (2) use 5 times of raw material of Chinese medicine quality;
(4) vacuum is subtracted: pass into the compressed air through purification, make pressure in pressure reduction extractor rise to 0Mpa gradually, take 2 minutes;
(5) pressurize: continue to pass into the compressed air through purification, make the pressure in pressure reduction extractor reach 3MPa, keep 3 minutes; ;
(6) reduce pressure: stop pressurization, start evacuation, make pressure in pressure reduction extractor be down to 0Mpa;
(7) circulation is extracted: continue step (3) ~ (6), circulate 6 times;
(8) be separated, concentrated: carry out solid-liquid separation by centrifugal, the hollow fiber filter that centrifugal liquid is 1.0 μm by aperture is filtered to clarification; Filtrate concentrated, being concentrated into relative density is 1.02(25 DEG C of measurement, with the density of water for benchmark);
(9) step (2) gained volatile effective component and fat-soluble effective ingredient are mixed homogeneously with step (8) gained concentrated solution, obtain the compound Chinese medicine extract II for whitening skin and preserving moisture.
Embodiment 3
(1) by 40 mass parts Rhizoma Chuanxiongs, 20 mass parts Herba Houttuyniae and 15 mass parts Rhizoma Cyperis respectively dry, pulverize and sieve, obtain coarse powder; Gained coarse powder is put into extraction kettle, adopts CO
2supercritical fluid extraction, separated and collected volatile effective component and fat-soluble effective ingredient; Described CO
2the extraction temperature of supercritical fluid extraction 45 DEG C, extract pressure 35Mpa, extraction time is 150min, CO
2flow velocity is 20L/h, separation temperature 45 DEG C, collects volatile effective component and fat-soluble effective ingredient under separating pressure 7Mpa;
(2) by 40 mass parts Pseudobulbus Bletillae (Rhizoma Bletillae), 25 mass parts Rhizoma Alismatis and 15 mass parts Fructus Ligustri Lucidi respectively dry, pulverize and sieve, obtain coarse powder; By gained coarse powder and step (1) through CO
2medicinal residues after supercritical fluid extraction are placed in pressure reduction extractor; Described pressure reduction extractor adopts condensed water temperature at 4 DEG C;
(3) evacuation: in 30 seconds, utilizes vacuum station evacuation, makes to form vacuum in pressure reduction extractor, vacuum degree control is at-50MPa ~-100MPa, keep 3 minutes, spray into the aqueous solution of 15 DEG C, its quality be equivalent to step (1) and (2) use 5 times of raw material of Chinese medicine quality;
(4) vacuum is subtracted: pass into the compressed air through purification, make pressure in pressure reduction extractor rise to 0Mpa gradually, take 2 minutes;
(5) pressurize: continue to pass into the compressed air through purification, make the pressure in pressure reduction extractor reach 3MPa, keep 3 minutes; ;
(6) reduce pressure: stop pressurization, start evacuation, make pressure in pressure reduction extractor be down to 0Mpa;
(7) circulation is extracted: continue step (3) ~ (6), circulate 6 times;
(8) be separated, concentrated: carry out solid-liquid separation by centrifugal, the hollow fiber filter that centrifugal liquid is 1.0 μm by aperture is filtered to clarification; Filtrate concentrated, being concentrated into relative density is 1.02(25 DEG C of measurement, with the density of water for benchmark);
(9) step (2) gained volatile effective component and fat-soluble effective ingredient are mixed homogeneously with step (8) gained concentrated solution, obtain the compound Chinese medicine extract III for whitening skin and preserving moisture.
Embodiment 4
(1) by 45 mass parts Rhizoma Chuanxiongs, 25 mass parts Herba Houttuyniae and 20 mass parts Rhizoma Cyperis respectively dry, pulverize and sieve, obtain coarse powder; Gained coarse powder is put into extraction kettle, adopts CO
2supercritical fluid extraction, separated and collected volatile effective component and fat-soluble effective ingredient; Described CO
2the extraction temperature of supercritical fluid extraction 35 DEG C, extract pressure 20Mpa, extraction time is 3h min, CO
2flow velocity is 25L/h; Described separation adopts temperature 35 DEG C, and pressure is 3Mpa;
(2) by 45 mass parts Pseudobulbus Bletillae (Rhizoma Bletillae), 30 mass parts Rhizoma Alismatis and 20 mass parts Fructus Ligustri Lucidi respectively dry, pulverize and sieve, obtain coarse powder; By gained coarse powder and step (1) through CO
2medicinal residues after supercritical fluid extraction are placed in pressure reduction extractor; Described pressure reduction extractor adopts condensed water temperature at 2 DEG C;
(3) evacuation: in 20 seconds, utilizes vacuum station evacuation, makes to form vacuum in pressure reduction extractor, vacuum degree control is at-50MPa ~-100MPa, keep 2 minutes, spray into the aqueous solution of 15 DEG C, its quality be equivalent to step (1) and (2) use 5 times of raw material of Chinese medicine quality;
(4) vacuum is subtracted: pass into the compressed air through purification, make pressure in pressure reduction extractor rise to 0Mpa gradually, take 1 minute;
(5) pressurize: continue to pass into the compressed air through purification, make the pressure in pressure reduction extractor reach 3MPa, keep 2 minutes; ;
(6) reduce pressure: stop pressurization, start evacuation, make pressure in pressure reduction extractor be down to 0Mpa;
(7) circulation is extracted: continue step (3) ~ (6), circulate 6 times;
(8) be separated, concentrated: carry out solid-liquid separation by centrifugal, the hollow fiber filter that centrifugal liquid is 1.0 μm by aperture is filtered to clarification; Filtrate concentrated, being concentrated into relative density is 1.02(25 DEG C of measurement, with the density of water for benchmark);
(9) step (2) gained volatile effective component and fat-soluble effective ingredient are mixed homogeneously with step (8) gained concentrated solution, obtain the compound Chinese medicine extract IV for whitening skin and preserving moisture.
Embodiment 5
(1) by 50 mass parts Rhizoma Chuanxiongs, 15 mass parts Herba Houttuyniae and 10 mass parts Rhizoma Cyperis respectively dry, pulverize and sieve, obtain coarse powder; Gained coarse powder is put into extraction kettle, adopts CO
2supercritical fluid extraction, separated and collected volatile effective component and fat-soluble effective ingredient; Described CO
2the extraction temperature of supercritical fluid extraction 55 DEG C, extract pressure 50Mpa, extraction time is 1h, CO
2flow velocity is 10L/h; Described separation adopts temperature 55 DEG C, and pressure is 10Mpa;
(2) by 50 mass parts Pseudobulbus Bletillae (Rhizoma Bletillae), 50 mass parts Rhizoma Alismatis and 10 mass parts Fructus Ligustri Lucidi respectively dry, pulverize and sieve, obtain coarse powder; By gained coarse powder and step (1) through CO
2medicinal residues after supercritical fluid extraction are placed in pressure reduction extractor; Described pressure reduction extractor adopts condensed water temperature at 7 DEG C;
(3) evacuation: in 50 seconds, utilizes vacuum station evacuation, makes to form vacuum in pressure reduction extractor, vacuum degree control is at-50MPa ~-100MPa, keep 5 minutes, spray into the aqueous solution of 15 DEG C, its quality be equivalent to step (1) and (2) use 5 times of raw material of Chinese medicine quality;
(4) vacuum is subtracted: pass into the compressed air through purification, make pressure in pressure reduction extractor rise to 0Mpa gradually, take 3 minutes;
(5) pressurize: continue to pass into the compressed air through purification, make the pressure in pressure reduction extractor reach 3MPa, keep 5 minutes; ;
(6) reduce pressure: stop pressurization, start evacuation, make pressure in pressure reduction extractor be down to 0Mpa;
(7) circulation is extracted: continue step (3) ~ (6), circulate 5 times;
(8) be separated, concentrated: carry out solid-liquid separation by centrifugal, the hollow fiber filter that centrifugal liquid is 1.0 μm by aperture is filtered to clarification; Filtrate concentrated, being concentrated into relative density is 1.02(25 DEG C of measurement, with the density of water for benchmark);
(9) step (2) gained volatile effective component and fat-soluble effective ingredient are mixed homogeneously with step (8) gained concentrated solution, obtain the compound Chinese medicine extract V for whitening skin and preserving moisture.
Comparative example 1
(1) by 40 mass parts Rhizoma Chuanxiongs and 15 mass parts Rhizoma Cyperis respectively dry, pulverize and sieve, obtain coarse powder; Gained coarse powder is put into extraction kettle, adopts CO
2supercritical fluid extraction, separated and collected volatile effective component and fat-soluble effective ingredient; Described CO
2the extraction temperature of supercritical fluid extraction 45 DEG C, extract pressure 35Mpa, extraction time is 150min, CO
2flow velocity is 20L/h; Described separation adopts temperature 45 C, and pressure is 7Mpa;
(2) by 25 mass parts Rhizoma Alismatis and 15 mass parts Fructus Ligustri Lucidi respectively dry, pulverize and sieve, obtain coarse powder; By gained coarse powder and step (1) through CO
2medicinal residues after supercritical fluid extraction are placed in pressure reduction extractor; Described pressure reduction extractor adopts condensed water temperature at 4 DEG C;
(3) evacuation: in 30 seconds, utilizes vacuum station evacuation, makes to form vacuum in pressure reduction extractor, vacuum degree control is at-50MPa ~-100MPa, keep 3 minutes, spray into the aqueous solution of 15 DEG C, its quality be equivalent to step (1) and (2) use 5 times of raw material of Chinese medicine quality;
(4) vacuum is subtracted: pass into the compressed air through purification, make pressure in pressure reduction extractor rise to 0Mpa gradually, take 2 minutes;
(5) pressurize: continue to pass into the compressed air through purification, make the pressure in pressure reduction extractor reach 3MPa, keep 3 minutes;
(6) reduce pressure: stop pressurization, start evacuation, make pressure in pressure reduction extractor be down to 0Mpa;
(7) circulation is extracted: continue step (3) ~ (6), circulate 7 times;
(8) be separated, concentrated: carry out solid-liquid separation by centrifugal, the hollow fiber filter that centrifugal liquid is 1.0 μm by aperture is filtered to clarification; Filtrate concentrated, being concentrated into relative density is 1.02(25 DEG C of measurement, with the density of water for benchmark);
(9) step (2) gained volatile effective component and fat-soluble effective ingredient are mixed homogeneously with step (8) gained concentrated solution, obtain the compound Chinese medicine extract VI for whitening skin and preserving moisture
Comparative example 2
(1) by 20 mass parts Herba Houttuyniae and 15 mass parts Rhizoma Cyperis respectively dry, pulverize and sieve, obtain coarse powder; Gained coarse powder is put into extraction kettle, adopts CO
2supercritical fluid extraction, separated and collected volatile effective component and fat-soluble effective ingredient; Described CO
2the extraction temperature of supercritical fluid extraction 45 DEG C, extract pressure 35Mpa, extraction time is 150min, CO
2flow velocity is 20L/h; Described separation adopts temperature 45 C, and pressure is 7Mpa;
(2) by 40 mass parts Pseudobulbus Bletillae (Rhizoma Bletillae) and 15 mass parts Fructus Ligustri Lucidi respectively dry, pulverize and sieve, obtain coarse powder; By gained coarse powder and step (1) through CO
2medicinal residues after supercritical fluid extraction are placed in pressure reduction extractor; Described pressure reduction extractor adopts condensed water temperature at 4 DEG C;
(3) evacuation: in 30 seconds, utilizes vacuum station evacuation, makes to form vacuum in pressure reduction extractor, vacuum degree control is at-50MPa ~-100MPa, keep 3 minutes, spray into the aqueous solution of 15 DEG C, its quality be equivalent to step (1) and (2) use 5 times of raw material of Chinese medicine quality;
(4) vacuum is subtracted: pass into the compressed air through purification, make pressure in pressure reduction extractor rise to 0Mpa gradually, take 2 minutes;
(5) pressurize: continue to pass into the compressed air through purification, make the pressure in pressure reduction extractor reach 3MPa, keep 3 minutes; ;
(6) reduce pressure: stop pressurization, start evacuation, make pressure in pressure reduction extractor be down to 0Mpa;
(7) circulation is extracted: continue step (3) ~ (6), circulate 6 times;
(8) be separated, concentrated: carry out solid-liquid separation by centrifugal, the hollow fiber filter that centrifugal liquid is 1.0 μm by aperture is filtered to clarification; Filtrate concentrated, being concentrated into relative density is 1.02(25 DEG C of measurement, with the density of water for benchmark);
(9) step (2) gained volatile effective component and fat-soluble effective ingredient are mixed homogeneously with step (8) gained concentrated solution, obtain the compound Chinese medicine extract VII for whitening skin and preserving moisture
Comparative example 3
(1) by 40 mass parts Rhizoma Chuanxiongs and 20 mass parts Herba Houttuyniae respectively dry, pulverize and sieve, obtain coarse powder; Gained coarse powder is put into extraction kettle, adopts CO
2supercritical fluid extraction, separated and collected volatile effective component and fat-soluble effective ingredient; Described CO
2the extraction temperature of supercritical fluid extraction 45 DEG C, extract pressure 35Mpa, extraction time is 150min, CO
2flow velocity is 20L/h; Described separation adopts temperature 45 C, and pressure is 7Mpa;
(2) by 40 mass parts Pseudobulbus Bletillae (Rhizoma Bletillae) and 25 mass parts Rhizoma Alismatis respectively dry, pulverize and sieve, obtain coarse powder; By gained coarse powder and step (1) through CO
2medicinal residues after supercritical fluid extraction are placed in pressure reduction extractor; Described pressure reduction extractor adopts condensed water temperature at 4 DEG C;
(3) evacuation: in 30 seconds, utilizes vacuum station evacuation, makes to form vacuum in pressure reduction extractor, vacuum degree control is at-50MPa ~-100MPa, keep 3 minutes, spray into the aqueous solution of 15 DEG C, its quality be equivalent to step (1) and (2) use 5 times of raw material of Chinese medicine quality;
(4) vacuum is subtracted: pass into the compressed air through purification, make pressure in pressure reduction extractor rise to 0Mpa gradually, take 2 minutes;
(5) pressurize: continue to pass into the compressed air through purification, make the pressure in pressure reduction extractor reach 3MPa, keep 3 minutes; ;
(6) reduce pressure: stop pressurization, start evacuation, make pressure in pressure reduction extractor be down to 0Mpa;
(7) circulation is extracted: continue step (3) ~ (6), circulate 6 times;
(8) be separated, concentrated: carry out solid-liquid separation by centrifugal, the hollow fiber filter that centrifugal liquid is 1.0 μm by aperture is filtered to clarification; Filtrate concentrated, being concentrated into relative density is 1.02(25 DEG C of measurement, with the density of water for benchmark);
(9) step (2) gained volatile effective component and fat-soluble effective ingredient are mixed homogeneously with step (8) gained concentrated solution, obtain the compound Chinese medicine extract VIII for whitening skin and preserving moisture
Effect example:
One, detection method
Respectively previous embodiment gained compound Chinese medicine extract is mixed with the active ingredient solution that concentration is mass volume ratio 1% with dimethyl sulfoxide, carries out following experiment.
(1) mensuration of free radical scavenging effect
Getting active ingredient solution 2ml and concentration is that diphenyl picryl phenylhydrazine (DPPH) solution (solvent of this solution the is water) 2ml of 100 μm of ol/L successively adds in same tool plug test tube, shakes up; Left at room temperature 30 minutes take solvent as blank working sample absorbance under 517nm wavelength.
Suppression ratio: K%=[1-(Ai-Aj)/Ac] × 100%;
The absorbance of Ai:2ml DPPH solution+2ml active ingredient solution;
The absorbance of Aj:2ml active ingredient solution+2ml solvent (i.e. dimethyl sulfoxide);
The absorbance of Ac:2ml DPPH solution+2ml solvent (i.e. dimethyl sulfoxide).
(2) anti-ultraviolet property measures
In 280 ~ 400nm wave-length coverage, scan every 10nm, determine the light transmittance of active ingredient solution under each wavelength, calculate the average absorption rate of wave-length coverage.
(3) tyrosine vitality test in mouse melanin cell is suppressed
Be inoculated in 96 orifice plates by the B16 mouse melanin cell (Chinese Academy of Sciences's cell bank) being in exponential phase, culture medium is the calf serum of RPMI1640+ percent by volume 10%, every hole 100 μ l(10
4individual cell/well), put 37 DEG C, 5%CO
2after cultivating 24h in incubator, add the active ingredient solution 50 μ l after filtration sterilization.Continue to cultivate after 3d, abandoning supernatant is the 0.15%(w/v of 6.8 with pH value) poly butylene succinate rinses twice, every hole adds 90 μ l containing 1%(volume fraction) the PBS(pH value 7.4 of Triton X-100).Ultrasonication in ice bath, every hole adds the L-3,4 dihydroxyphenylalanine (L-dopa) of 10 μ l10mmol/L, hatches 60min for 37 DEG C, and in 475nm place colorimetric, blank well returns to zero, and surveys each hole absorbance.
Suppression ratio=(1-medicine group mean absorbance values+matched group mean absorbance values) × 100%
(4) B16 cell test in mouse melanin cell is suppressed
By B16 mouse black-in tumor cell with 1 × 10
5the density of individual/ml is inoculated in the culture dish that diameter is 9cm, at 37 DEG C, 5%CO
2condition under cultivate 2 days, abandon supernatant, add the active ingredient solution 18-22ml after filtration sterilization, then cultivate 3 days, abandon supernatant, PBS washs, every hole adds the trypsin solution peptic cell 3min of 0.5ml mass percent 0.2%, and every ware adds 2ml maintenance medium and stops digestion.After piping and druming mixing, often kind of concentration is taken out 0.5ml and is made cell counting.All the other cell suspension, with the centrifugal 5min of 2500r/min, abandon supernatant, in precipitation, add NaOH solution, and heating makes melanin dissolve, and select the enzyme-linked immunosorbent assay instrument of 490nm wavelength surveys absorbance.
B16 cell suppression ratio=[1-(medicine hole absorbance+medicine porocyte density)+(control wells absorbance+control wells cell density)] × 100%
(5) detection of moistening effect:
Keratodermatitis hydration rate is tested: operation principle take capacitor as instrument probe, because water is the material that on skin, dielectric constant is maximum, when skin moisture content changes, the capacitance of skin also changes, so can by measuring skin pricktest capacitance, analyzing skin surface moisture content.Conventional instrument is Corneometer CM825.By measuring the change using the change of keratodermatitis capacitance before and after product to weigh water content of stratum corneum, thus moisture-keeping efficacy this kind of method evaluating cosmetics carries out quantification to the moisture of keratodermatitis, the change of skin moisture content can be reacted delicately, and favorable reproducibility, be one of method that current moisture-keeping cosmetics efficacy assessments is conventional.
Experimental technique: bend side at candidate left and right forearm and mark 3 × 3cm
2the square Experimental Area of size, using left arm as the test zone of moisturizer, the corresponding symmetrical region of right arm is blank, then detects the moisture at each test position with Corneometer CM825 test, repeats 5 times respectively, draw meansigma methods.By formula (1) calculated hydration rate.
Two, experimental result:
(1) mensuration of free radical scavenging effect, result is as shown in table 1:
Table 1 free radical scavenging effect testing result
| Suppression ratio (%) | |
| Embodiment 1 | 87.4 |
| Embodiment 2 | 88.5 |
| Embodiment 3 | 88.2 |
| Embodiment 4 | 88.8 |
| Embodiment 5 | 87.9 |
| Comparative example 1 | 75.7 |
| Comparative example 2 | 65.8 |
| Comparative example 3 | 80.1 |
(2) anti-ultraviolet property measures, and result is as shown in table 2:
Table 2 anti-ultraviolet property measurement result
| Absorbance (%) | |
| Embodiment 1 | 47.6 |
| Embodiment 2 | 51.0 |
| Embodiment 3 | 51.8 |
| Embodiment 4 | 49.9 |
| Embodiment 5 | 48.5 |
| Comparative example 1 | 25.6 |
| Comparative example 2 | 40.2 |
| Comparative example 3 | 43.5 |
(3) suppress tyrosine vitality test in mouse melanin cell, result is as shown in table 3:
Table 3 tyrosine vitality test result
| Suppression ratio (%) | |
| Embodiment 1 | 54.7 |
| Embodiment 2 | 55.9 |
| Embodiment 3 | 56.7 |
| Embodiment 4 | 56.1 |
| Embodiment 5 | 55.7 |
| Comparative example 1 | 43.8 |
| Comparative example 2 | 32.7 |
| Comparative example 3 | 48.2 |
(4) suppress B16 cell test in mouse melanin cell, result is as shown in table 4:
Table 4 B16 cell result of the test
| Suppression ratio (%) | |
| Embodiment 1 | 58.6 |
| Embodiment 2 | 61.1 |
| Embodiment 3 | 62.3 |
| Embodiment 4 | 59.8 |
| Embodiment 5 | 58.8 |
| Comparative example 1 | 51.1 |
| Comparative example 2 | 40.3 |
| Comparative example 3 | 53.9 |
(5) detection of moistening effect, result is as shown in table 5:
Table 5 moistening effect experimental result
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| 1h | 80% | 81% | 83% | 84% | 82% | 58% | 73% | 75% |
| 2h | 73% | 75% | 76% | 73% | 72% | 42% | 66% | 69% |
| 4h | 52% | 53% | 55% | 50% | 52% | 36% | 44% | 48% |
| 6h | 48% | 46% | 48% | 48% | 49% | 29% | 37% | 43% |
| 8h | 43% | 45% | 44% | 45% | 47% | 25% | 33% | 40 |
Above-mentioned experimental result shows, compound Chinese medicine extract of the present invention can effectively restraint of tyrosinase activity, suppression black generate, and has scavenging free radicals and anti-ultraviolet function, whitening skin and preserving moisture Be very effective simultaneously.
Application implementation example
(1) preparation of whitening skin and preserving moisture frost
The present invention gets above-mentioned compound Chinese medicine extract and is mixed with whitening skin and preserving moisture frost, formula following (by mass percentage):
The processing technology of whitening skin and preserving moisture frost is as follows:
(1) in oil phase pot, add the Jojoba oil in oil phase A, Butyrospermum parkii fruit fat, Cyclomethicone, polydimethylsiloxane, glyceryl stearate, cetearyl alcohol, sad/triglyceride, PEG-20 methyl sesquistearate, methyl sesquistearate and four-(dibutyl alkyl hydrocinnamic acid) pentaerythritol ester, be heated to 85 DEG C while stirring;
(2) in aqueous phase pot, add deionized water, carbomer, pantothenylol, glycerol and the EDETATE SODIUM in aqueous phase B, be heated to 85 DEG C while stirring;
(3) to emulsifying pot to carry out after evacuation oil pumping respectively mutually and aqueous phase equal to the homogeneous stirring of emulsifying pot;
(4) after temperature is down to 65 DEG C, add C phase triethanolamine homogenizing again, (daily) essence in D phase is added after being cooled to 45 DEG C, Methylisothiazolinone, adds E phase compound Chinese medicine extract (above-described embodiment and comparative example prepare) under stirring;
(5) stop stirring after temperature is down to 36 DEG C, discharging, obtains compound Chinese medicine extract whitening skin and preserving moisture moisturiser.
Whitening skin and preserving moisture frost is respectively by compound natural plant extract I, II, III, IV, V obtained whitening skin and preserving moisture frost I, II, III, IV, V according to the method described above.
The above-mentioned whitening skin and preserving moisture frost prepared is carried out clinic trial observation.
The object of observation: 20 ~ 45 years old age women.Choosing face has women 20 example of pigementation in various degree, the problem skin such as dark yellow, dry.
Observational technique: before sleep every night after cleaning skin, coats above-mentioned whitening skin and preserving moisture frost.Continuous use 28 days is a course for the treatment of.
Criterion of therapeutical effect: (1) is effective: dark yellow the disappearing of pigment reaches more than 90%, skin has obvious whitening to moisten smooth feeling; (2) effective: pigment is dark yellow disappears more than 50%, and skin is moistened smooth compared with whitening; (3) invalid: unchanged before and after treatment.Result is as shown in table 6:
| Effective | Effectively | Invalid | Total effective rate | |
| Whitening skin and preserving moisture frost I | 14 | 3 | 3 | 85% |
| Whitening skin and preserving moisture frost II | 15 | 2 | 3 | 85% |
| Whitening skin and preserving moisture frost III | 16 | 3 | 1 | 95% |
| Whitening skin and preserving moisture frost IV | 17 | 1 | 2 | 90% |
| Whitening skin and preserving moisture frost V | 15 | 3 | 2 | 90% |
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from spirit of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (7)
1. there is the Chinese medicinal compound extract of whitening skin and preserving moisture effect, it is characterized in that: this Chinese medicinal compound extract is prepared from by following raw material of Chinese medicine according to the mass fraction:
2. a kind of Chinese medicinal compound extract with whitening skin and preserving moisture effect according to claim 1, is characterized in that: this Chinese medicinal compound extract is prepared from by following raw material of Chinese medicine according to the mass fraction:
3. a kind of Chinese medicinal compound extract with whitening skin and preserving moisture effect according to claim 1 is preparing the application in whitening skin and preserving moisture skin care item.
4. application according to claim 3, is characterized in that: described in there is whitening skin and preserving moisture effect the mass percentage of Chinese medicinal compound extract in whitening skin and preserving moisture skin care item be 0.1 ~ 20%.
5. application according to claim 3, is characterized in that: described in there is whitening skin and preserving moisture effect the mass percentage of Chinese medicinal compound extract in whitening skin and preserving moisture skin care item be 1 ~ 8%.
6. application according to claim 3, is characterized in that: containing whitening agent, wetting agent, antioxidant, surfactant, alcohol compound or water in described whitening skin and preserving moisture skin care item.
7. application according to claim 3, is characterized in that: described whitening skin and preserving moisture skin care item are solution, suspension, emulsion, cream, paste, gel, milk, skin care liquid, fancy soap, oil, foundation emulsion, wet-milling or spray.
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