A kind of skin care item containing Chinese spun gold extract
[technical field]
The invention belongs to cosmetics technical field, be specifically related to a kind of skin care item containing Chinese spun gold extract.
[background technology]
Everybody has a love of beauty.Aspect antidotal skin care item, people always give and suitable attention.According to human senility free radical theory, superfluous free radical can cause body injury, and unsaturated fatty acid is oxidized to superoxides, forms lipofuscin.Oxygen-derived free radicals too much also can destroy cell membrane and other important component, makes protein and enzyme denaturation.Can make like this that human body skin shows as that skin is nonelastic, matt, senile plaque and xerosis cutis, desquamation, wrinkle etc.
Lichens is the homobium being made up of certain mushroom and algae, is a group plant that occupies special status on botany.The metabolite of lichens, so-called lichens composition is different from the composition of other multiple high or rudimentary plants completely, belongs to a kind of particular category chemically.Concrete condition is by carried out classifying (towards Binet, Chai Tianzhu " chemistry of lichens composition ", 1948 years) towards people such as Binets.
The one that discloses Chinese patent 92102210.7 contains safe, and the skin care item of the stable material can check melanin generating, and these skin care item contain the material that one or two or more kinds the check melanin that extracts from lichens cell culture generates; Wherein the lichens plant before cell culture belongs to Parmeliaceae, Usneaceae, Graphidaceae, Lecideaceae, Umbilicariaceae, Lecanoraceae.This patent Introduction by lichens extract for the skin care item of whitening.
Scientific literature: Wang LS, Narui T, Harada H, Culberson WL2001:Ethnic uses of lichens in Yunnan, China, The Bryologist104 (3): 345-349 has introduced lichens China's spun gold and spun gold silk ball is applied for a long time on the ground such as Yunnan, Tibet of China as health tea, the medical value that tool is high and edibility.But lichens extract, for antidotal skin care item, be there is not yet to report.
[summary of the invention]
The object of the present invention is to provide a kind of anti-aging skin care product containing Chinese spun gold extract.
Object of the present invention is achieved through the following technical solutions: a kind of skin care item containing Chinese spun gold extract, and described skin care item comprise substrate and active component, and its active component comprises lichens extract, and described lichens is Chinese spun gold.
Preferably, described lichens also comprises spun gold silk ball.
Another optimal way, described active component also comprises Radix Angelicae Sinensis, the Radix Astragali, Radix Ginseng, Semen Vitis viniferae, Radix Glycyrrhizae and Herb Gynostemmae Pentaphylli.
An optimal way again, described substrate comprises one or more in deionized water, glycerol, carbomer, dexpanthenol, the different pelargonate of cetearyl alcohol, triglyceride, titanium dioxide, squalane, triethanolamine, glyceryl stearate, PSI, EDETATE SODIUM, hyaluronate sodium, lanoline, Cera Flava, vaseline, Pulvis Talci, titanium dioxide, calcium carbonate, starch, ethanol, ethyl acetate, mink oil, palm wax, candelilla wax, Jojoba wax polyvinylpyrrolidone.
One is preferred again, contains the Chinese spun gold extract of 0.01~20% mass fraction in described skin care item total amount, is more preferably 2~5%.
Skin care item containing Chinese spun gold extract of the present invention, the Chinese spun gold extract of described lichens is made by following methods:
(1) described lichens is pulverized, added ethanol water to mix;
(2) above-mentioned material liquid mixture is extracted under ul-trasonic irradiation, hyperacoustic work efficiency is 18~25KHz, extracts 30~50 DEG C of temperature, extraction time 30~50min; Extracting liquid filtering is centrifugal, obtains filtrate;
(3) will in filtering residue, add ethanol water, repeating step (2), filter centrifugal, collect filtrate;
(4) combining step (2) and step (3) gained filtrate, concentrating under reduced pressure also reclaims ethanol;
(5) in the concentrated solution of step (4) gained, add ethanol, then be adjusted to concentration of alcohol be 85%~90%, 0~2 DEG C leave standstill 24~48 hours;
(6) step (5) gained is left standstill to liquid centrifugalize, collect supernatant, be evaporated to step (5) gained leave standstill liquid long-pending 1/10~1/20, through D152 resin isolation, with 55~65% ethanol elutions, and collect ethanol elution part, and dry, obtain lichens extract.
The preparation method of the described skin care item containing Chinese spun gold extract, comprises the following steps:
Active component using lichens China spun gold extract as skin care item, chooses substrate according to the dosage form of skin care item, obtains the described skin care item containing Chinese spun gold extract after composite.
The preparation method of the described preferred skin care item containing Chinese spun gold extract, comprises following steps:
(1) by lichens, Radix Angelicae Sinensis, the Radix Astragali, Radix Ginseng, Semen Vitis viniferae, Radix Glycyrrhizae, Herb Gynostemmae Pentaphylli is pulverized, and adds ethanol water to mix;
(2) above-mentioned material liquid mixture is extracted under ul-trasonic irradiation, hyperacoustic work efficiency is 18~25KHz, extracts 30~50 DEG C of temperature, extraction time 30~50min; Extracting liquid filtering is centrifugal, obtains filtrate;
(3) will in filtering residue, add ethanol water, repeating step (2), filter centrifugal, collect filtrate;
(4) combining step (2) and step (3) gained filtrate, concentrating under reduced pressure also reclaims ethanol;
(5) in the concentrated solution of step (4) gained, add dehydrated alcohol, then be adjusted to concentration of alcohol be 85%~90%, 0~2 DEG C leave standstill 24~48 hours;
(6) step (5) gained is left standstill to liquid centrifugalize, collects supernatant, be evaporated to step (5) gained leave standstill liquid long-pending 1/10~1/20, through D152 resin isolation, with 55~65% ethanol elutions, and collect ethanol elution part, dry, obtain extract;
(7) extract step (6) being obtained, as the active component of skin care item, is chosen substrate according to the dosage form of skin care item, obtains the described skin care item containing Chinese spun gold extract after composite.
Described skin care item are solution, suspension, emulsion, cream, paste, gel, milk, skin care liquid, powder, fancy soap, oil, dry powder, foundation emulsion, wet-milling or spray.
The preparation method of the described skin care item containing Chinese spun gold extract, is more preferably:
Ethanol water described in step (1) is the mass fraction ethanol that is 65%~75%; The consumption of described ethanol water is calculated by mass, for the 6-10 that is equivalent to coarse powder quality doubly;
Ethanol described in step (5) is that percent by volume is more than or equal to 95% ethanol;
Centrifugalize rotating speed described in step (6) is preferably 6000~8000r/min.
Described skin care item are active component and substrate, add that the acceptable adjuvant of skin care field is prepared into other external use skin care dosage forms.Except above-mentioned essential composition, can suitably coordinate as required the composition conventionally using in skin care item.For example, the adjunct ingredient such as other whitening agent, wetting agent, antioxidant, surfactant, antiseptic, daily spice, alcohols, water.
The dosage form of the skin care item containing Chinese spun gold extract of the present invention is not particularly limited, and can be solution, suspension, emulsion, cream, paste, gel, milk, skin care liquid, powder, fancy soap, oil, dry powder, foundation emulsion, wet-milling or spray.
In addition,, not weakening in the scope of main effect of the present invention, except adding extract of the present invention, described composite skin care product can also contain with main effect of the present invention synergistic other composition.Described additive can carry out suitable selection and combination according to the preparation of described skin care item and object by those skilled in the art.
For example, in order to strengthen described effect, described composite skin care product can contain skin absorption promoter.
In addition, described composite skin care product can also contain a kind of material selecting in the group that free water soluble vitamins, fat soluble vitamin, polypeptide, polysaccharide, sphingolipid and Sargassum extract form.
In addition, as required, composite skin care product of the present invention can also contain the additive conventionally joining in composite skin care product except containing essential composition.The example of described additive comprises oiliness composition, wetting agent, emollient, surfactant, organic and inorganic pigment, organic dust, UV absorbent, antiseptic, antibacterial, antioxidant, pH controlling agent, alcohol, coloring agent, spice, blood circulation accelerant, freshener, antiperspirant and pure water etc.
Composite skin care product of the present invention can be made preparation, and described preparation is not particularly limited.For example, described preparation can comprise emulsion, cream, skin cream, essence, facial film, gel, powder, lip pomade, skin care item substrate, foundation cream, washing liquid (lotion), ointment, patch, beautifying liquid, decontamination foam, cleaning cream, clean water, soap or spray.
Chinese spun gold of the present invention is the one of lichens, and form is: thallus is thread or hair-like, reaches 250mm, compared with thin and soft, cylindrical or oblate, filamentous surface is netted rib ridge, the dirty white of base portion, upwards reddish brown Huang or orange colour gradually, tarnish, branch body y-bend is without branch, slightly curling, top is point gradually, without sorodium.Its chemical composition: mainly containing atranorine (Atranorin, C
19h
18o
8) and unknown material (TLC).The chemical composition that it contains: atranorine (Atranorin, C
19h
18o
8), belonging to the nascent metabolite of cup fungi, it has the healthy cell of enhancing immunocompetence, improves immunity of organisms, anoxia enduring, constitutional effect, also has certain active anticancer effect simultaneously; Gyrophoric acid belongs to the nascent metabolite of Mycophyta, the effect that possess antioxidation, improves body immunity equally.In China's spun gold, contain abundant fungus polysaccharide, scalable body immunity, human activin cytokine, slow down aging also contains abundant atranorine, Ka Nali element and three archils simultaneously; Its nutritional labeling contains: crude protein, crude fat, total sugar; In aminoacid composition taking glutamic acid and Aspartic Acid as main; In abundant mineral element, iron content is high; Carotenoid species are abundant.
Ferulic acid in Radix Angelicae Sinensis is the effective ingredient of antioxidation; by direct elimination free radical; suppress membrane lipid peroxidization and radical reaction that superoxide radical causes; and with the infringement of the multiple mechanism antagonism radical pair tissues such as biological film phospholipid join protection membrane lipid; gloomy or the raw skin ulcer of complexion due to free radical retardance is swollen, have specially good effect.
The Radix Astragali contains several amino acids, the various trace elements of needed by human and folic acid, so with the Radix Astragali additive as skin care item, there is comprehensive Nutrition, can increase skin-nourishing, prevent skin aging, reduce wrinkle of skin.
Radix Ginseng has tonification and antidotal effect, can improve the activity of superoxide dismutase in body and in epiderm skin cell, strengthen the elimination of active oxidation free radical, stop the generation of harmful substance in body, improve the metabolism of body, reduce or eliminate the lipofuscin that produces and stagnate in body.The leachate of ginsenoside in Radix Ginseng can slowly be absorbed by skin; to skin without any pessimal stimulation; the blood capillary of energy skin expansion, promotes cutaneous circulation, strengthens skin-nourishing; prevent skin dehydration, sclerosis, wrinkling; thereby strengthen the elasticity of skin, made cell obtain new life, bright and clean and moist to protection skin; prevent presenility, played beautification function.
The extract of Semen Vitis viniferae has the new and effective Natural antioxidant material that can not synthesize in a kind of human body.It is antioxidation, the strongest material of removing free radical ability that current occurring in nature is found, its antioxidant activity is 50 times, ascorbic 20 times of the plain E of dimension, it can effectively remove unnecessary free radical in human body, has the effect of superpower slow down aging and enhancing immunity.
Effective ingredient licoflavone in Radix Glycyrrhizae, the effectively generation of check melanin, reaches the effect of whitening.Licoflavone also has the multiple free radical of removing and suppresses lipofuscin generation, promote the several functions such as Antioxidative Defense System, be improved body microcirculation, regulate organism endocrine system, rising RBC number, health invigorating, slow down aging, alleviate the effects such as pigmentation.
The extract of Herb Gynostemmae Pentaphylli is gypenoside, and it has defying age, promotes the physiological actions such as cell metabolism, skin care.Japan Patent report, gypenoside, for the isolated cell of fetus and adult's cell, has obvious cell survival prolongation effect, can effectively prevent and treat skin aging, is applicable to configure defying age cream frost.
The present invention has following advantage and beneficial effect with respect to prior art: the Chinese spun gold extract of lichens of the present invention has good antioxidation, formation that can control volume endoperoxides lipofuscin, the effectively aging of delaying skin, can increase skin activity, reduces wrinkle; In epidermis cell is cultivated, the Chinese spun gold extract of above-mentioned lichens can obviously change sebum composition, thereby reduces the oil of skin, improves the soft and moist degree of skin.The mode that adopts ultrasonic extraction, extracts effective ingredient in raw material quickly and efficiently, and extraction efficiency is high, and extraction time is short, extracts temperature low, extracts medicinal liquid impurity few, and extraction process operating cost is low, and overall economic efficiency is remarkable.The described skin care item containing lichens China spun gold extract are not because containing chemicals composition, and safe, nontoxic, little to skin irritation, stable in properties, can improve skin metabolism, plays antidotal effect.
[detailed description of the invention]
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
Embodiment 1
(1) Chinese spun gold is pulverized, adding mass fraction is 65%, and the ethanol water that lichens volume is 6 times mixes;
(2) above-mentioned material liquid mixture is extracted under ul-trasonic irradiation, hyperacoustic work efficiency is 25KHz, extracts 30 DEG C of temperature, extraction time 50min; Extracting liquid filtering is centrifugal, obtains filtrate;
(3) will in filtering residue, add the ethanol water of 6 times of filtering residue volumes, repeating step (2), filter centrifugal, collect filtrate;
(4) combining step (2) and step (3) gained filtrate, be evaporated to filtrate volume 1/10 and reclaim ethanol;
(5) in the concentrated solution of step (4) gained, add dehydrated alcohol, then be adjusted to concentration of alcohol be 85%, 0 DEG C leave standstill 24 hours;
(6) step (5) gained is left standstill to liquid centrifugalize, collects supernatant, be evaporated to step (5) gained leave standstill liquid long-pending 1/10, through D152 resin isolation, use 65% ethanol elution, and collect ethanol elution part, dry, obtain lichens extract.
(7) active component using Chinese spun gold extract as skin care item, chooses substrate and is, obtains the described skin care item containing Chinese spun gold extract after composite.The mass fraction of the lichens extract containing in described skin care item is 10%.
Embodiment 2
(1) Chinese spun gold is pulverized, adding mass fraction is 60%, and the ethanol water that lichens volume is 6 times mixes;
(2) above-mentioned material liquid mixture is extracted under ul-trasonic irradiation, hyperacoustic work efficiency is 20KHz, extracts temperature 50 C, extraction time 40min; Extracting liquid filtering is centrifugal, obtains filtrate;
(3) will in filtering residue, add ethanol water, repeating step (2), filter centrifugal, collect filtrate;
(4) combining step (2) and step (3) gained filtrate, concentrating under reduced pressure also reclaims ethanol;
(5) in the concentrated solution of step (4) gained, add ethanol, then be adjusted to concentration of alcohol be 90%, 2 DEG C leave standstill 48 hours;
(6) step (5) gained is left standstill to liquid centrifugalize, collects supernatant, be evaporated to step (5) gained leave standstill liquid long-pending 1/20, through D152 resin isolation, use 55% ethanol elution, and collect ethanol elution part, dry, obtain lichens extract.
(7) active component using lichens extract as skin care item, chooses substrate according to the dosage form of skin care item, obtains the described skin care item containing Chinese spun gold extract after composite.The mass fraction of the lichens extract containing in described skin care item is 5%.
Embodiment 3
(1) Chinese spun gold is pulverized, added ethanol water to mix;
(2) above-mentioned material liquid mixture is extracted under ul-trasonic irradiation, hyperacoustic work efficiency is 18KHz, extracts 30 DEG C of temperature, extraction time 50min; Extracting liquid filtering is centrifugal, obtains filtrate;
(3) will in filtering residue, add ethanol water, repeating step (2), filter centrifugal, collect filtrate;
(4) combining step (2) and step (3) gained filtrate, concentrating under reduced pressure also reclaims ethanol;
(5) in the concentrated solution of step (4) gained, add ethanol, then be adjusted to concentration of alcohol be 90%, 0 DEG C leave standstill 30 hours;
(6) step (5) gained is left standstill to liquid centrifugalize, collects supernatant, be evaporated to step (5) gained leave standstill liquid long-pending 1/15, through D152 resin isolation, use 65% ethanol elution, and collect ethanol elution part, dry, obtain lichens extract.
(7) active component using lichens extract as skin care item, chooses substrate according to the dosage form of skin care item, obtains the described skin care item containing Chinese spun gold extract after composite.The mass fraction of the lichens extract containing in described skin care item is 2%.
Embodiment 4
(1) by Chinese spun gold, Radix Angelicae Sinensis, the Radix Astragali, Radix Ginseng, Semen Vitis viniferae, Radix Glycyrrhizae, Herb Gynostemmae Pentaphylli is pulverized, and adds ethanol water to mix;
(2) above-mentioned material liquid mixture is extracted under ul-trasonic irradiation, hyperacoustic work efficiency is 18KHz, extracts 30 DEG C of temperature, extraction time 50min; Extracting liquid filtering is centrifugal, obtains filtrate;
(3) will in filtering residue, add ethanol water, repeating step (2), filter centrifugal, collect filtrate;
(4) combining step (2) and step (3) gained filtrate, concentrating under reduced pressure also reclaims ethanol;
(5) in the concentrated solution of step (4) gained, add ethanol, then be adjusted to concentration of alcohol be 90%, 0 DEG C leave standstill 30 hours;
(6) step (5) gained is left standstill to liquid centrifugalize, collects supernatant, be evaporated to step (5) gained leave standstill liquid long-pending 1/15, through D152 resin isolation, use 65% ethanol elution, and collect ethanol elution part, dry, obtain lichens extract.
(7) active component using lichens extract as skin care item, chooses substrate according to the dosage form of skin care item, obtains the described skin care item containing Chinese spun gold extract after composite.The mass fraction of the lichens extract containing in described skin care item is 2%.
Embodiment 5
(1) by Chinese spun gold, Radix Angelicae Sinensis, the Radix Astragali, Radix Ginseng, Semen Vitis viniferae, Radix Glycyrrhizae, Herb Gynostemmae Pentaphylli is pulverized, and adds ethanol water to mix;
(2) above-mentioned material liquid mixture is extracted under ul-trasonic irradiation, hyperacoustic work efficiency is 25KHz, extracts 30 DEG C of temperature, extraction time 50min; Extracting liquid filtering is centrifugal, obtains filtrate;
(3) will in filtering residue, add the ethanol water of 6 times of filtering residue volumes, repeating step (2), filter centrifugal, collect filtrate;
(4) combining step (2) and step (3) gained filtrate, be evaporated to filtrate volume 1/10 and reclaim ethanol;
(5) in the concentrated solution of step (4) gained, add dehydrated alcohol, then be adjusted to concentration of alcohol be 85%, 0 DEG C leave standstill 24 hours;
(6) step (5) gained is left standstill to liquid centrifugalize, collects supernatant, be evaporated to step (5) gained leave standstill liquid long-pending 1/10, through D152 resin isolation, use 65% ethanol elution, and collect ethanol elution part, dry, obtain lichens extract.
(7) active component using lichens extract as skin care item, chooses substrate according to the dosage form of skin care item, obtains the described skin care item containing Chinese spun gold extract after composite.The mass fraction of the lichens extract containing in described skin care item is 10%.
Effect embodiment:
One, detection method
Respectively the lichens extract of previous embodiment is mixed with to the active ingredient solution that concentration is mass volume ratio 1% with dimethyl sulfoxide, carries out following experiment.
(1) mensuration of free radical scavenging effect
Diphenyl picryl phenylhydrazine (DPPH) solution (solvent of this solution is the to be water) 2ml that gets active ingredient solution 2ml and concentration and be 100 μ mol/L successively adds in same tool plug test tube, shakes up; Under room temperature, leave standstill 30 minutes, taking solvent as blank working sample absorbance under 517nm wavelength.
Suppression ratio: K%=[1-(Ai-Aj)/Ac] × 100%;
The absorbance of Ai:2ml DPPH solution+2ml active ingredient solution;
The absorbance of Aj:2ml active ingredient solution+2ml solvent (being dimethyl sulfoxide);
The absorbance of Ac:2ml DPPH solution+2ml solvent (being dimethyl sulfoxide).
(2) anti-ultraviolet property is measured
In 280-400nm wave-length coverage, scan every 10nm, determine the light transmittance of active ingredient solution under each wavelength, calculate the average absorption rate of wave-length coverage.
(3) suppress tyrosine vitality test in mouse melanin cell
To be inoculated in 96 orifice plates in the B16 of exponential phase mouse melanin cell (Chinese Academy of Sciences's cell bank), culture medium is the calf serum of RPMI1640+ percent by volume 10%, every hole 100 μ l (10
4individual cell/well), put 37 DEG C, 5%CO
2in incubator, cultivate after 24h, add the active ingredient solution 50 μ l after filtration sterilization.Continue to cultivate after 3d, 0.15% (w/v) poly butylene succinate that abandoning supernatant is 6.8 with pH value rinses twice, and every hole adds the PBS (pH value 7.4) of 90 μ l containing 1% (volume fraction) Triton X-100.Ultrasonication in ice bath, every hole adds the L-3,4 dihydroxyphenylalanine (L-dopa) of 10 μ l10mmol/L, hatches 60min for 37 DEG C, and in 475nm place colorimetric, blank well zeroing, surveys each hole absorbance.
Suppression ratio=(1-medicine group mean light absorbency value+matched group mean light absorbency value) × 100%
(4) suppress melanocyte synthetic test in mouse melanin cell
By B16 mouse black-in tumor cell with 1 × 10
5the density of individual/ml is inoculated in the culture dish that diameter is 9cm, at 37 DEG C, and 5%CO
2condition under cultivate 2 days, abandon supernatant, add the active ingredient solution 18-22ml after filtration sterilization, then cultivate 3 days, abandon supernatant, PBS washing, every hole adds the pancreatin solution peptic cell 3min of 0.5ml mass percent 0.2%, and every ware adds 2ml maintenance medium and stops digestion.After piping and druming mixes, every kind of concentration is taken out 0.5ml and is made cell counting.All the other cell suspension, with the centrifugal 5min of 2500r/min, are abandoned supernatant, in precipitation, add NaOH solution, and heating is dissolved melanin, on the enzyme-linked immunosorbent assay instrument of selection 490nm wavelength, survey absorbance.
Melanin synthesizes suppression ratio=[1-(medicine hole absorbance+medicine porocyte density)+(control wells absorbance+control wells cell density)] × 100%
Two, experimental result
(1) mensuration of free radical scavenging effect, result is as shown in table 1:
Table 1
(2) anti-ultraviolet property is measured, and result is as shown in table 2:
Table 2
(3) suppress tyrosine vitality test in mouse melanin cell, result is as shown in table 3:
Table 3
(4) suppress melanocyte synthetic test in mouse melanin cell, result is as shown: as shown in the of 4:
Table 4
Application Example 1
Lichens extract prepared by embodiment 1 is applied in moisturiser, wherein, lichens extract 2 mass parts, glyceryl stearate 6 mass parts, triglyceride 5 mass parts, PSI 4 mass parts, different pelargonate 3 mass parts of cetearyl alcohol, glycerol 2 mass parts, carbomer 0.5 mass parts, dexpanthenol dexpanthenol 5 mass parts, triethanolamine 0.5 mass parts, EDETATE SODIUM 0.1 mass parts, hyaluronate sodium 0.1 mass parts and disodium glycyrrhizinate 0.1 mass parts, daily essence 0.1 mass parts, DMDM Hydantoin 0.2 mass parts, water is supplemented to 100 mass parts.Wherein: the different pelargonate of glyceryl stearate, triglyceride, PSI and cetearyl alcohol is A component; Water, glycerol, carbomer, dexpanthenol and EDETATE SODIUM are B component; Triethanolamine is C component; Hyaluronate sodium, disodium glycyrrhizinate, daily essence and DMDM Hydantoin are D component.Lichens extract is dissolved in to B component, is then heated to respectively 85 DEG C with A component; C component is added to B component, stir, then A component is added in the aforementioned B component obtaining and C component mixed phase, after homogenizing, be cooled to 45 DEG C and add D component, 36 DEG C stop stirring discharging, obtain moisturiser.
The moisturiser preparing is carried out to clinic trial observation.
The object of observation: 20-45 age, middle-aged women year.Choosing face has female middle-aged 100 examples of the pigments such as chloasma in various degree, butterfly spot, senile plaque.
Observational technique: after cleaning skin, coat above-mentioned moisturiser before sleep every night.Using continuously 28 days is a course for the treatment of.
Criterion of therapeutical effect: (1) is effective: melanin disappears and reaches more than 90%, skin has significantly moist smooth feeling; (2) effective: melanin disappears more than 50%, skin is moist smooth; (3) invalid: unchanged before and after treatment.Result is as shown in table 5:
Table 5
Application Example 2
Lichens extract prepared by embodiment 2 is applied in emulsion, wherein, lichens extract 4 mass parts, glyceryl stearate 6 mass parts, triglyceride 5 mass parts, PSI 4 mass parts, different pelargonate 3 mass parts of cetearyl alcohol, glycerol 2 mass parts, carbomer 0.5 mass parts, dexpanthenol 4 mass parts, triethanolamine 0.5 mass parts, EDETATE SODIUM 0.1 mass parts, hyaluronate sodium 0.1 mass parts and disodium glycyrrhizinate 0.1 mass parts, daily essence 0.1 mass parts, DMDM Hydantoin 0.2 mass parts, water is supplemented to 100 mass parts.Wherein: the different pelargonate of glyceryl stearate, triglyceride, PSI and cetearyl alcohol is A component; Water, glycerol, carbomer, dexpanthenol dexpanthenol and EDETATE SODIUM are B component; Triethanolamine is C component; Hyaluronate sodium, disodium glycyrrhizinate, daily essence and DMDM Hydantoin are D component.Lichens extract is dissolved in to B component, is then heated to respectively 85 DEG C with A component; C component is added to B component, stir, then A component is added in the aforementioned B component obtaining and C component mixed phase, after homogenizing, be cooled to 45 DEG C and add D component, 36 DEG C stop stirring discharging, obtain emulsion.
The emulsion preparing is carried out to clinic trial observation.
The object of observation: 20-45 age, middle-aged women year.Choosing face has female middle-aged 100 examples of the pigments such as chloasma in various degree, butterfly spot, senile plaque.
Observational technique: after cleaning skin, coat above-mentioned emulsion before sleep every night.Using continuously 28 days is a course for the treatment of.
Criterion of therapeutical effect: (1) is effective: melanin disappears and reaches more than 90%, skin has significantly moist smooth feeling; (2) effective: melanin disappears more than 50%, skin is moist smooth; (3) invalid: unchanged before and after treatment.Result is as shown in table 6:
Table 6
Above-described embodiment is preferably embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spirit of the present invention and principle, substitutes, combination, simplify; all should be equivalent substitute mode, within being included in protection scope of the present invention.