CN106620295B - Chinese herbal medicine composition - Google Patents
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- CN106620295B CN106620295B CN201510429056.3A CN201510429056A CN106620295B CN 106620295 B CN106620295 B CN 106620295B CN 201510429056 A CN201510429056 A CN 201510429056A CN 106620295 B CN106620295 B CN 106620295B
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Abstract
The invention discloses a Chinese herbal medicine composition, which is extracted from the following raw material medicines in parts by weight: 2-4 parts of scutellaria baicalensis; 1-3 parts of radix ophiopogonis; 1-3 parts of lophatherum gracile; 6-8 parts of ginseng. The invention also relates to the application of the Chinese herbal medicine composition in cosmetics.
Description
Technical Field
The invention relates to the field of natural medicinal chemistry and cosmetics, in particular to a Chinese herbal medicine composition with an oil secretion regulating effect, an antioxidant effect, an anti-inflammatory effect and a moisturizing effect. After the Chinese herbal medicine composition is applied to the skin, the skin can be helped to achieve a skin-balancing state of refreshing.
Background
The sebaceous membrane is a lipid membrane formed on the surface of skin by the emulsification of endogenous sebum secreted by sebaceous glands and exogenous sebum produced by the disintegration of epidermal cells, moisture and sweat, and plays an important role in keeping the moisture on the surface of the skin, regulating the pH value of the skin, keeping the epidermis intact, promoting the absorption of topical drugs and cosmetics, and the like. There are many factors that affect sebaceous gland secretion, including sex, age, hormone level, diet, physiological cycle, season, and environment, among others. Sebum secretion is excessive, so that the skin appearance is dirty, oily and rough, pores are large, and skin diseases such as acne and inflammation are easily caused; when sebum secretion is too small, various problems such as dry skin and roughness may occur. Therefore, effective regulation and repair of the sebaceous gland's lipid secretion function is the first step in establishing the skin barrier function.
When the sebaceous glands are overactive, in addition to the appearance of a coarse pore, purely oily skin, there are also other common skin problems, such as acne and seborrheic dermatitis. Therefore, effective regulation of sebaceous gland secretion, and controlled discharge of oil and fat are important for maintaining healthy neutral skin conditions. Most of numerical control oil daily chemical products in the market at present only simply clean grease, emphasize water replenishing and moisture keeping, shrink pores through electrolytes, can only relieve oily skin to a certain extent, but cannot enable the skin to get rid of oily light really.
Oxidation is the biggest threat to skin aging and is the root of all skin problems. When skin cells are oxidized, free radicals are generated to invade and destroy collagen of the skin, so that the skin loses elasticity and luster, and the skin has the skin problems of dark yellow, water shortage, wrinkle and the like. There are many factors causing skin oxidation, and besides the internal factors of the body, many external factors such as computer radiation, ultraviolet rays, pressure, environmental pollution and the like are easy to generate excessive free radicals, so that the cell vitality and the metabolic function are reduced, and the phenomena of skin oxidation and aging are caused. Therefore, oxidation resistance is the essence of beauty and skin care, and is the basis for keeping skin moisture and whitening.
At present, the removal of excess free radicals is often achieved by antioxidants, which are roughly divided into two categories: one class is enzyme antioxidants such as superoxide dismutase (SOD), glutathione peroxidase, catalase, coenzyme Q, etc.; and the other is non-enzyme antioxidant, such as vitamin E, vitamin C, selenium and compounds thereof. Some natural antioxidants such as green tea, grape juice, pomegranate and other plant extracts, and rutin, baicalin and other natural flavonoids also have significant antioxidant effects. At present, the antioxidant activity is mainly studied by DPPH method, superoxide anion radical method, hydroxyl radical method, thiobarbituric acid method (TBARS), ABTS+Among them, the DPPH method is commonly used for the research and evaluation of the antioxidant activity of test samples because it comprehensively reflects the strength of the antioxidant activity of a substance.
Inflammation is a defense reaction of active tissues in the body against and eliminating injury factors, and meanwhile, the occurrence of various diseases is closely related to inflammation, such as skin inflammation, atherosclerosis, diabetes, arthritis, malignant tumor and the like. Inflammation of the skin can cause a certain degree of damage to the skin, thereby causing various inflammatory skin diseases, and the most common skin inflammation is acne, namely 'whelk'. Research shows that various skin diseases are caused by bacterial infection, inflammation and the like, so that the antibacterial and anti-inflammatory tests become important pharmacodynamic indexes for researching and developing beauty and health care products.
The action mechanism of the anti-inflammatory drug mainly comprises the regulation of an endocrine system, the inhibition of the release of cyclooxygenase COX-2, the regulation of the function of an immune system, the elimination of oxygen radicals in vivo and the like. Researches find that Chinese herbal medicines with the effects of clearing heat, removing toxicity, clearing heat and drying dampness, such as coptis chinensis, scutellaria baicalensis, cortex moutan, honeysuckle and the like, can play the anti-inflammatory activity of the Chinese herbal medicines through the action mechanism.
The barrier function of skin to protect internal tissues depends on the hydration and moisturizing ability of skin, and the water storage capacity of skin accounts for 18% -20% of the whole body. The skin moisture is excessively lost due to factors such as temperature change and pressure caused by air conditioning, environmental pollution and season change, skin aging, metabolism slowing and the like, so that the skin is rough and dull, dry lines are formed, and various skin problems are caused. Preventing excessive loss of skin moisture is important to maintain good skin barrier function and skin health, and even the beautiful appearance of skin.
In order to maintain moisture in the skin and reduce excessive moisture loss, a moisturizing ingredient is generally added to the skin preparation for external use. There are many kinds of moisturizers, and common moisturizing ingredients can be classified into two categories, namely closed moisturizers and hygroscopic moisturizers. The occlusive moisturizer has the function of keeping a certain moisture content of the stratum corneum by forming a film on the skin surface to prevent the evaporation of water from the skin, and includes mineral oil and wax such as vaseline, vegetable oil and animal fat such as lanolin, phospholipid, cholesterol, etc. The hygroscopic humectant is similar to a natural moisturizing factor in epidermis, and can be strongly combined with water to achieve the effect of moisturizing the horny layer. Such moisturizing ingredients are: natural moisturizing factor, protein, polysaccharide, ceramide, various vitamins, etc. The moisturizers of animal and mineral sources easily cause problems of skin allergy, skin irritation and the like, and the natural moisturizers searched from plants meet the requirements of people on cosmetics of natural origin.
Ginseng (panaxginng c.a.mey.), also known as shencao and herba hyperici japonici, is the dry root of ginseng of araliaceae, and is first recorded in shennong's herbal medicine, and has the efficacies of tonifying the five internal organs, calming the spirit, calming the soul, stopping palpitation, eliminating pathogenic qi, improving eyesight, and benefiting intelligence, which is the "top grade" of Chinese herbal medicines. The ginseng contains various bioactive substances such as polysaccharide, saponin, volatile oil, polypeptide, amino acid, organic acid, flavone, alkaloid and the like, wherein the ginsenoside is a main active component of the ginseng, related research reports are relatively complete, and the ginseng polysaccharide also has various pharmacological activities. Pharmacological research shows that ginseng has a bidirectional regulating effect on a central nervous system and a cardiovascular system, can obviously enhance the immunity of an organism, regulate an endocrine system and influence the metabolism of substances, and in addition, ginseng also has various pharmacological effects of protecting the liver, resisting tumors and the like, and has extremely high medicinal value. In the aspect of beauty and health care, the ginsenoside in the ginseng has the functions of skin care, oxidation resistance and ultraviolet resistance, and can effectively increase the activity of antioxidant enzyme in human keratinocytes and inhibit lipid peroxidation, thereby resisting skin photoaging, protecting human skin and delaying aging. In addition, the ginseng extract has strong bacteriostatic and anti-inflammatory effects, is a natural additive of high-grade cosmetics, and has obvious effects on freckles, chloasma and senile skin pigmentation.
Scutellaria baicalensis Georgi (Scutellaria baicalensis Georgi) is also used as the root of camellia, is the dry root of Scutellaria baicalensis Georgi of Labiatae, has the effects of clearing heat and drying dampness, purging fire and removing toxicity, stopping bleeding, preventing miscarriage and the like, and has multiple pharmacological actions of resisting bacteria, resisting viruses, resisting inflammation, resisting oxidation, resisting aging, protecting liver, benefiting gallbladder and the like according to modern pharmacological research. The scutellaria contains various components, and the main active component of the scutellaria is a flavonoid compound. It is reported that baicalin has significant anti-photodamage and anti-inflammatory effects, and has protective effects on skin damaged by ultraviolet radiation; baicalin, baicalein, wogonin and the like have obvious effects of resisting inflammation, resisting oxidation and the like.
Radix Ophiopogonis (Ophiopogon japonicus), also known as Ophiopogon japonicus and Oenothera japonica, is root tuber of Ophiopogon japonicus of Liliaceae, is sweet in nature and slightly bitter in taste, has effects of nourishing yin and moistening lung, benefiting stomach and promoting fluid production, clearing heart fire and relieving restlessness, and can be used together with radix asparagi and snow pear to cook soup to have effects of nourishing yin and moistening lung, and moistening skin and reducing weight. The ophiopogon root contains polysaccharide, multiple saponins and flavonoids compounds, the ophiopogon root polysaccharide and the ophiopogon root total saponin have obvious influence on the cardiovascular system and the immune system, the homoisoflavone component contained in the ophiopogon root can effectively remove free radicals so as to play a role in resisting oxidation, and the ophiopogon root polysaccharide also has multiple pharmacological activities of reducing blood sugar, protecting gastric mucosa and the like. Experimental research shows that the radix ophiopogonis aqueous extract has the effects of resisting free radicals, resisting lipid peroxidation, reducing plasma concentration and the like, thereby playing the roles of resisting aging and prolonging life.
Lophatherum gracile (Lophatherum gracile), also used as Lophatherum gracile Ophiopogon japonicus and Jinji rice, is a dry stem and leaf of Lophatherum gracile of Gramineae, has cold nature and sweet and light taste, and has the effects of clearing heat and purging fire, relieving restlessness and quenching thirst, and inducing diuresis and treating stranguria. The lophatherum gracile leaves contain various active ingredients, wherein the flavonoid ingredients have various pharmacological activities of bacteriostasis, antioxidation, vasoconstriction, liver protection, antivirus, myocardial protection and the like, and the polysaccharide has obvious effect of clearing various in-vitro free radicals. The volatile components in the lophatherum gracile leaves enable the lophatherum gracile leaves to have unique fresh scent, and can be used for research and development in the fields of food additives and cosmetics. In addition, the lophatherum gracile leaves also contain rich substances such as tea polyphenol, chlorophyll and the like, so that the lophatherum gracile has remarkable antioxidant activity. In addition, researches show that the lophatherum gracile extract has better tyrosinase inhibition capability, so that the formation of melanin is reduced, and the skin whitening effect is achieved.
Analyzing the traditional Chinese medicine theory background: ginseng: it is a monarch drug to help the skin to clear qi and ascend hair, and also to promote the production of body fluid, remove heat and discharge turbidity, and condition oily skin. Scutellaria baicalensis: is bitter and cold in nature and good at clearing away heat from the upper energizer skin, and is used as a ministerial drug for eliminating dampness and dissolving turbidity and purifying turbidity and greasiness. The book Ben Cao Zheng (herbal medicine) carries the root of large-flowered skullcap: it is especially effective in dispelling heat from the skin surface. Radix ophiopogonis: the product for promoting the production of body fluid and moistening dryness is an adjuvant drug for moistening and repairing the damaged skin barrier. Herba lophatheri: it is a guiding drug that can clear and disperse the pathogenic wind, help the skin clear qi and ascend, direct the whole formula to the body surface, dispel heat and clean the skin. The effects of ascending clear and descending turbid are achieved, so that the skin is dredged and smoothened, the self-cleaning function is exerted orderly, and the effects of permeability, cleanness and balance are achieved.
However, the literature does not mention the compound Chinese herbal medicine composition prepared by the scutellaria baicalensis, the ophiopogon root, the lophatherum gracile and/or the ginseng. Therefore, the invention provides a Chinese herbal medicine composition for the first time, and the Chinese herbal medicine composition has the comprehensive effects of regulating the secretion of grease, resisting oxidation, resisting inflammation and preserving moisture. The Chinese herbal medicine composition is used as an active additive to be applied to cosmetics, and can help to achieve a skin balancing state of refreshing. The balance skin state of the refreshing is just the result of the comprehensive effects of the Chinese herbal medicine composition such as oil secretion regulation, oxidation resistance, inflammation resistance, moisture retention and the like.
Disclosure of Invention
The invention is based on the theoretical background of 'gas' and the need of controlling oil at the same time, and several Chinese herbal medicines are used to form a compound Chinese herbal medicine composition according to the monarch, minister, assistant and guide. The Chinese herbal medicine composition can be used as an active additive to be applied to products to help achieve a skin-balancing state of refreshing.
Therefore, in one aspect of the present invention, a Chinese herbal medicine composition is provided, which is extracted from the following raw material herbs:
2-4 parts of scutellaria baicalensis
1-3 parts of dwarf lilyturf tuber
1-3 parts of lophatherum gracile
In a preferred embodiment, the herbal composition of the present invention further comprises 6 to 8 parts by weight of ginseng.
In one embodiment, the extraction of the herbal composition of the present invention comprises the steps of:
a) soaking radix Scutellariae, radix Ophiopogonis, and folium Bambusae, and extracting to obtain extract A;
b) soaking radix Ginseng, and extracting to obtain extract B;
c) combining the extract A and the extract B to obtain the Chinese herbal medicine composition.
In some embodiments, said step a) or b) comprises vehicle soaking. In some embodiments, the vehicle is selected from: water, ethanol solutions, polyol solutions, and other solvents of similar polarity.
In some embodiments, the extracting of step a) or b) comprises: decocting, reflux extracting, soaking or percolating.
In a specific embodiment, the extraction of the herbal composition of the present invention comprises the steps of:
a) soaking Scutellariae radix, radix Ophiopogonis, and folium Bambusae in suitable solvent for about 0.5-1 hr, extracting by corresponding method, collecting filtrate, standing, filtering, concentrating to about 2.5 times (V/W) of total amount of medicinal materials, and making into solution containing crude drug 0.1-0.5 g/ml;
b) soaking Ginseng radix in suitable solvent for about 0.5-1h, extracting by corresponding method, collecting filtrate, standing, filtering, concentrating to about 1 time (V/W) of total amount of the materials, and making into solution containing crude drug 0.1-0.5 g/ml;
c) mixing the extract obtained in step a) and the extract obtained in step b) in proportion to obtain oil-controlling compound extract containing crude drug 0.1-0.5 g/ml.
In the invention, the traditional Chinese medicinal materials in the formula can be properly enlarged or reduced according to the proportion. Suitable solvents include water (distilled, deionized, purified), ethanol solutions of varying concentrations, polyol solutions of varying concentrations, and other solvents of similar polarity; the extraction method can be decocting, reflux extracting, soaking, percolating, etc.
In another aspect of the invention, the invention provides an application of a Chinese herbal medicine composition in regulating oil secretion, resisting oxidation, resisting inflammation and moisturizing, wherein the Chinese herbal medicine composition is extracted from the following raw material medicines in proportion:
2-4 parts of scutellaria baicalensis
1-3 parts of dwarf lilyturf tuber
1-3 parts of lophatherum gracile.
Preferably, the Chinese herbal medicine composition also protects 6-8 parts by weight of ginseng.
In another aspect of the present invention, a cosmetic is provided, which comprises a herbal composition and a cosmetically acceptable excipient, wherein the herbal composition is extracted from the following raw materials:
2-4 parts of scutellaria baicalensis
1-3 parts of dwarf lilyturf tuber
1-3 parts of lophatherum gracile.
Preferably, the herbal composition also protects 6-8 parts by weight of ginseng in some preferred embodiments, the herbal composition is used in the cosmetic in an amount of 0.001-20% (w/w), preferably 0.008-10% (w/w), more preferably 0.01-5% (w/w), and most preferably 0.01-2% (w/w), based on the total weight of the cosmetic.
The extract of the Chinese herbal medicine composition can be applied to skin external preparations, health-care foods and medicines, and particularly can be applied to cosmetics, and different dosage is added according to different types of preparations.
In another aspect of the present invention, there is provided a skin external preparation having lipid secretion regulating and/or antioxidant and/or anti-inflammatory and/or moisturizing and antioxidant effects, comprising an extract of the herbal composition prepared according to the method of the present invention and a cosmetically acceptable vehicle. In some embodiments, the extract of the herbal composition is selected from the group consisting of: face cleaning lotion, cosmetic water, lotion, cream, jelly and facial mask. In a preferred embodiment, the extract of the herbal composition is contained in the external preparation for skin in an amount of 0.001-20% (w/w). Preferably 0.008-10% (w/w), more preferably 0.01-5% (w/w), and most preferably 0.01-2% (w/w).
The composition for external preparation for skin is a general concept of all ingredients generally used for the external part of skin, and may be, for example, a cosmetic composition or a pharmaceutical composition. The cosmetic composition may be a basic cosmetic, a face makeup cosmetic, a body cosmetic, a hair care cosmetic, etc., and the formulation thereof is not particularly limited and may be appropriately selected depending on the purpose.
The cosmetic composition also contains different cosmetically acceptable media or matrix excipients according to different formulations and purposes.
The cosmetically, dermatologically or pharmaceutically acceptable vehicle that can be used in the composition for external application to skin of the present invention is in the form of a water phase, an oil phase, a gel, a wax-in-water emulsion, an oil-in-water emulsion or a water-in-oil emulsion. The aqueous phase is a mixture of one or more water-soluble or dispersible components, which may be liquid, semi-solid, or solid at room temperature (25 ℃). The vehicle includes or may be in the form of a suspension, dispersion or solution in an aqueous or hydro-alcoholic vehicle, which may contain a thickening or gelling agent. The person skilled in the art can select suitable product forms, the components contained therein, based on the knowledge of the person skilled in the art.
The composition may comprise an aqueous phase which may contain water or a mixture of water and at least one hydrophilic organic solvent such as an alcohol, in particular a linear or branched lower monohydric alcohol containing from 2 to 5 carbon atoms, such as ethanol or propanol; polyols, such as propylene glycol, sorbitol, glycerol, panthenol or polyethylene glycols and mixtures thereof.
When the composition of the invention is in the form of an emulsion, the composition may also optionally comprise a surfactant.
The composition may also comprise film-forming polymers such as polyurethanes, polyacrylic acid homo-or copolymers, polyesters, hydrocarbon-based resins and/or silicone resins. The polymer may be dissolved or dispersed in a cosmetically acceptable vehicle and optionally combined with a plasticizer.
The compositions of the present invention may also comprise an oil phase containing oil-soluble or oil-dispersible components that are liquid at room temperature (25 ℃) and/or substances that are oily or waxy at room temperature, such as waxes, semisolids, gums, and mixtures thereof. The oil phase may also contain an organic solvent.
Typically liquid at room temperature, suitable oily substances include: hydrocarbon-based oils of animal origin, such as perhydrosqualene; hydrocarbon-based vegetable oils, such as liquid triglycerides of C4-10 fatty acids, e.g. heptanoic acid or octanoic acid triglycerides, or oils, e.g. sunflower oil, corn oil, soybean oil, grapeseed oil, castor oil, avocado oil, octanoic/decanoic acid triglycerides, jojoba oil; linear or branched hydrocarbons of mineral or synthetic origin, such as liquid paraffin and its derivatives, vaseline; synthetic esters and ethers, in particular esters of fatty alcohols, such as isopropyl myristate, 2-ethylhexyl palmitate, 2-octyldodecyl stearate, isostearyl isostearate; hydroxylated esters, such as isostearyl lactate, octyl hydroxystearate, octyl dodecyl hydroxystearate, heptanoates, octanoates and decanoates of fatty alcohols; polyol esters such as propylene glycol dicaprylate, neopentyl glycol diheptanoate, diethylene glycol diisononanoate, and pentaerythritol esters; c12-26-containing fatty alcohols, such as octyldodecanol, 2-butyloctanol, 2-hexyldecanol, 2-undecylpentadecanol, oleyl alcohol; fluoro and/or fluorosilicone oils based in part on hydrocarbons, silicone oils, volatile or non-volatile linear or cyclic polymethylsiloxanes which are liquid or semi-solid at room temperature, such as cyclic polydimethylsiloxanes and polydimethylsiloxanes, optionally containing phenyl groups, such as phenyltrimethicones, silicones and mixtures thereof.
The composition of the present invention may further comprise any component commonly used in the cosmetic field. These components include preservatives, aqueous phase thickeners (extract biopolymers, synthetic polymers) and fatty phase thickeners, fragrances, hydrophilic and lipophilic active agents and mixtures thereof.
The compositions of the invention may also comprise an additional particulate phase, which may be a pigment and/or a pearlescent agent and/or a filler used in cosmetic compositions.
Pigments may be present in the composition, suitable inorganic pigments include titanium oxide, zirconium oxide and cerium oxide as well as zinc oxide, iron oxide and ferric blue; suitable organic pigments include barium, strontium, calcium and aluminum lakes and carbon black.
Pearling agents may be present in the composition, suitable pearling agents include mica coated with titanium oxide, iron oxide or natural pigments.
Fillers may be present in the composition, suitable fillers include talc, silica, zinc stearate, mica, kaolin, nylon powder, polyethylene powder, teflon, starch, boron nitride, copolymer microspheres, such as silicone resin microbeads.
The oil phase of the compositions of the present invention may comprise one or more waxes, gums or mixtures thereof. Waxes include hydrocarbon-based waxes, fluoro waxes, and/or silicone waxes, and may be derived from vegetable, mineral, animal, and/or synthetic sources. Suitable waxes include beeswax, carnauba wax, candelilla wax, paraffin wax, microcrystalline wax, ozokerite; synthetic waxes include polyethylene waxes, silicone waxes containing C16-45. Gums are generally polydimethylsiloxanes or sodium carboxymethylcellulose or extracts, and semisolid substances are generally hydrocarbon-based compounds, such as lanolin and its derivatives.
The compositions of the present invention may be formulated into any suitable product form. Such product forms include, but are not limited to, aerosol sprays, creams, lotions, solids, liquids, dispersions, foams, gels, lotions, mousses, ointments, powders, patches, pomades, solutions, hand pump sprays, sticks, masks and towelettes. The compositions of the present invention may be conveniently used to prepare or as cosmetic, dermatological or pharmaceutical topical products by various methods well known in the art.
The composition for external skin preparations of the present invention may include one or more of the following ingredients: anti-allergic agents, antimicrobial agents, antioxidants, chelating agents, colorant depigmenting agents, emollients, emulsifiers, exfoliants, film formers, fragrances, humectants, insect repellents, lubricants, pharmaceutically active agents, moisturizers, light stabilizers, preservatives, skin protectants, skin penetration enhancers, sunscreens, stabilizers, surfactants, thickeners, viscosity modifiers, vitamins, or any combination thereof.
Drawings
FIGS. 1A-1B show photomicrographs of blank well cells before and after drying. In which fig. 1A shows a micrograph of the blank well cells before drying. Figure 1B shows a micrograph of the blank well cells after drying.
Figures 2A-2B show photomicrographs of well cells with the addition of the positive control sodium hyaluronate before and after drying. Among them, fig. 2A shows a microphotograph of the well cells before drying, to which 0.1% sodium hyaluronate was added. Figure 2B shows a micrograph of the well cells after drying with 0.1% sodium hyaluronate added.
FIGS. 3A-3B show micrographs of the well cells with added ginseng before and after drying. Among them, FIG. 3A shows a microphotograph of well cells to which 5000ug/ml of ginseng was added before drying. FIG. 3B shows a micrograph of the well cells after drying with 5000ug/ml ginseng added.
FIGS. 4A-4B show micrographs of well cells before and after drying, to which the herbal composition of the present invention was added. Among them, FIG. 4A shows a microphotograph of the well cells added with 5000ug/ml of the herbal composition of the present invention before drying. FIG. 4B shows a micrograph of the well cells after drying, to which 5000ug/ml of the herbal composition of the present invention was added.
Detailed Description
The invention discloses a Chinese herbal medicine composition which is extracted from the raw materials of ginseng, scutellaria baicalensis, radix ophiopogonis and lophatherum gracile. Through relevant in vitro efficacy verification, the Chinese herbal medicine composition disclosed by the invention has the comprehensive efficacies of regulating oil secretion and/or resisting oxidation and/or resisting inflammation and/or preserving moisture, and simultaneously has the comprehensive efficacies of regulating oil secretion, resisting oxidation, resisting inflammation and preserving moisture.
The Chinese herbal medicine composition can be used as an effect additive to be added into cosmetics to help achieve a skin balancing state of refreshing.
The literature does not relate to the Chinese herbal medicine composition taking the herbs of ginseng, scutellaria baicalensis, ophiopogon root and lophatherum gracile as raw materials, and does not mention that the Chinese herbal medicine composition is added into cosmetics as a functional additive. Therefore, the invention provides the Chinese herbal medicine composition taking the above medicines as raw materials for the first time, and the Chinese herbal medicine composition has the unexpected technical effects of regulating the oil secretion and/or resisting oxidation and/or resisting inflammation and/or preserving moisture, and simultaneously has the comprehensive effects of regulating the oil secretion, resisting oxidation, resisting inflammation and preserving moisture.
In some embodiments, the herbal composition of the present invention may be used at a concentration of 5mg/ml or more, 6 mg/ml or more, 7 mg/ml or more, 8mg/ml or more, 9 mg/ml or more, 10mg/ml or more, 12 mg/ml or more, 16 mg/ml or more, 18 mg/ml or more. In some embodiments, the herbal composition of the present invention may be used at a concentration of 5-150 mg/ml, 6-150 mg/ml, 7-150 mg/ml, 8-150 mg/ml, 9-150 mg/ml, 10-150 mg/ml, 12-150 mg/ml, 16-150 mg/ml, 18-150 mg/ml. In some embodiments, the herbal composition of the present invention may be used at a concentration of 5-100 mg/ml, 6-100 mg/ml, 7-100 mg/ml, 8-100 mg/ml, 9-100 mg/ml, 10-100 mg/ml, 12-100 mg/ml, 16-100 mg/ml, 18-100 mg/ml. In some embodiments, the herbal composition of the present invention may be used at a concentration of 5-75 mg/ml, 6-75 mg/ml, 7-75 mg/ml, 8-75 mg/ml, 9-75 mg/ml, 10-75 mg/ml, 12-75 mg/ml, 16-75 mg/ml, 18-75 mg/ml. In some embodiments, the herbal composition of the present invention may be used at a concentration of 5-50 mg/ml, 6-50 mg/ml, 7-50 mg/ml, 8-50 mg/ml, 9-50 mg/ml, 10-50 mg/ml, 12-50 mg/ml, 16-50 mg/ml, 18-50 mg/ml.
The Chinese herbal medicine composition prepared in examples 1 to 3 of the present invention can be used for the preparation of external preparations for skin. The skin external preparation is preferably a cosmetic composition such as a lotion, essence, cream, etc. The weight percentage of the Chinese herbal medicine composition in the skin external preparation is 0.001-20% (w/w). Preferably 0.008-10% (w/w), more preferably 0.01-5% (w/w), and most preferably 0.01-2% (w/w).
The invention is further illustrated below with reference to specific examples. It is to be understood, however, that these examples are illustrative only and are not to be construed as limiting the scope of the present invention. Test methods in which specific conditions are not specified in the following examples are generally carried out under conventional conditions or under conditions recommended by the manufacturer. All percentages and parts are by weight unless otherwise indicated.
The sources of the Chinese herbal medicines used in the following examples are as follows:
example 1: preparation of the herbal composition of the invention
Weighing a proper amount of scutellaria baicalensis, ophiopogon japonicus and lophatherum gracile in a weight ratio of 4:1:3, extracting with 10 times of deionized water for 1 hour, filtering cotton for 2 times, combining filtrates, passing through a 50nm ceramic membrane, adding deionized water for full membrane separation to obtain dialysate, passing through an RO reverse osmosis membrane to obtain trapped fluid, and finally preparing into 0.2g/ml solution, namely the extract A1.
Weighing a proper amount of ginseng, crushing, soaking with 70% ethanol 10 times the amount of the ginseng overnight, filtering by using filter paper, concentrating the filtrate at 55 ℃, standing overnight at 4 ℃, filtering by using filter paper, and finally preparing into 0.2g/ml solution, namely the extract B1.
Extract A1 was mixed with extract B1 at a weight ratio of 1:1 to make up 0.2g/ml of the herbal composition of the present invention.
Example 2: preparation of the herbal composition of the invention
Weighing a proper amount of scutellaria baicalensis, ophiopogon japonicus and lophatherum gracile in a weight ratio of 3:2:1, extracting with 10 times of deionized water for 1 hour, filtering cotton for 2 times, combining filtrates, filtering with a 50nm ceramic membrane, adding deionized water for full membrane separation to obtain dialysate, passing through an RO reverse osmosis membrane to obtain trapped fluid, and finally preparing into 0.1g/ml solution, namely the extract A2.
Weighing a proper amount of ginseng, crushing, soaking with 70% ethanol 10 times the amount of the ginseng overnight, filtering by using filter paper, concentrating the filtrate at 55 ℃, standing overnight at 4 ℃, filtering by using filter paper, and finally preparing into 0.1g/ml solution, namely the extract B2.
Extract A2 was mixed with extract B2 at a weight ratio of 1:1 to make up 0.1g/ml of the herbal composition of the present invention.
Example 3: preparation of the herbal composition of the invention
Weighing a proper amount of scutellaria baicalensis, ophiopogon japonicus and lophatherum gracile in a weight ratio of 2:3:2, extracting with 10 times of deionized water for 1 hour, filtering cotton for 2 times, combining filtrates, filtering with a 50nm ceramic membrane, adding deionized water for full membrane separation to obtain dialysate, passing through an RO reverse osmosis membrane to obtain trapped fluid, and finally preparing into 0.1g/ml solution, namely the extract A3.
Weighing a proper amount of ginseng, crushing, soaking with 70% ethanol 10 times the amount of the ginseng overnight, filtering by using filter paper, concentrating the filtrate at 55 ℃, standing overnight at 4 ℃, filtering by using filter paper, and finally preparing into 0.1g/ml solution, namely the extract B3.
Extract A3 was mixed with extract B3 at a weight ratio of 1:1 to make up 0.1g/ml of the herbal composition of the present invention.
Example 45 α -reductase inhibition assay
5 α -reductase is an important enzyme involved in androgen metabolism, and its overactivity may promote abnormal increase of sebaceous gland function, increase sebaceous gland volume and secretion, thus causing skin problems such as large pores and oily skin, etc. by using HPLC to detect testosterone content, the activity of 5- α reductase can be reflected.
After the Chinese herbal medicine composition is concentrated to be dry, Tris buffer solution is used for preparing crude drug concentration of 10mg/ml and 5mg/ml respectively.
The protease content should be measured before each experiment and the volume of protease solution required to be added calculated.
Enzyme amount 0.03g (calculated as total protein content); the testosterone concentration of the system is 150 umol/L; the extraction process was performed by vortexing for 20 seconds, 2000 rpm, and centrifuging for 2min in parallel with the tube.
Addition amount of male-para-dutasteride: a100 mg/ml solution was prepared in advance, and 0.01ml was added to the system.
The concentration of ethanol in the system is higher than 2% or other solvents are brought in, a solvent control is needed, the experimental steps are consistent with those of a sample solution tube, and only a sample is not contained.
The composition and amount of the reaction system are shown in Table 1 below
TABLE 1
Table 2 shows the specific experimental procedures.
TABLE 2
The experimental results are as follows:
basal enzyme activity ═ T concentration before reaction-T concentration after reaction (no inhibitor)](reaction time. enzyme amount) (C0-C0')/(M)Enzyme×t)
Activity of enzyme after addition of inhibitor [ T concentration before reaction-T concentration after reaction (with inhibitor)](reaction time enzyme amount) (C0-C n)/(M)Enzyme×t)
Inhibition rate ═ (enzyme basal activity-activity of enzyme after addition of inhibitor)/enzyme basal activity × 100%, [1- (C0-Cn)/(C0-C0') ] × 100%
The results of the evaluation of the 5 α -reductase inhibition of the herbal composition of the invention are shown in Table 3.
TABLE 3
| Concentration of | Inhibition rate | Concentration of | Inhibition rate | |
| Example 1 composition | 10mg/ml | 37.04％ | 5mg/ml | 15.25％ |
| Example 2 composition | 10mg/ml | 62.91％ | 5mg/ml | 20.18％ |
| Example 3 composition | 10mg/ml | 42.79％ | 5mg/ml | 17.64％ |
| Example 1 extract A1 | 10mg/ml | 19.58％ | 5mg/ml | / |
| Example 2 extract A2 | 10mg/ml | 10.9％ | 5mg/ml | / |
| Example 3 extract A3 | 10mg/ml | 13.77％ | 5mg/ml | / |
In practical application, the Chinese herbal medicine composition obtained in the examples 1, 2 and 3 of the invention has certain 5 α -reductase inhibition effect, wherein the Chinese herbal medicine composition in the example 2 has the inhibition rate of 20.18% when the solid content is 5mg/ml, and has the inhibition activity of 62.91% when the solid content is 10 mg/ml.
Example 5: DPPH radical scavenging test
Free radicals are a class of active chemical species with very strong oxidizing power. Ultraviolet UVA/UVB irradiation can generate a large amount of free radicals, damage the organism and accelerate aging. The extracts of the examples were tested for in vitro antioxidant activity using DPPH free radical scavenging.
The extracts of the examples were diluted with distilled water to two concentrations of 0.08mg/ml and 0.4mg/ml, respectively. Then 2ml of the extract of example, 0.5ml of 500. mu. mol/L ethanol DPPH solution and 1.5ml of water were added to the same tube, shaken, left to stand, and the absorbance (OD) was measured at 517nm after 30min, and the corresponding mixture was used as a blank. Examples the rate of DPPH clearance by the extract was calculated according to the following formula:
wherein:
T-DPPH + absorbance of the extracts of the examples,
t0-absorbance of example extract + solvent,
c-absorbance of DPPH + solvent.
The evaluation results of the antioxidant capacity of the Chinese herbal medicine composition of the present invention are shown in table 4.
TABLE 4
| Concentration of | Free radical scavenging rate | Concentration of | Free radical scavenging rate | |
| Example 1 composition | 0.08mg/ml | 43.24％ | 0.4mg/ml | 75.05％ |
| Example 2 composition | 0.08mg/ml | 73.4％ | 0.4mg/ml | 93.94％ |
| Example 3 composition | 0.08mg/ml | 32.89％ | 0.4mg/ml | 69.64％ |
| Example 1 extract A1 | 0.08mg/ml | 69.58％ | 0.4mg/ml | 90.12％ |
| Example 2 extract A2 | 0.08mg/ml | 40.35％ | 0.4mg/ml | 75.86％ |
| Example 3 extract A3 | 0.08mg/ml | 49.17％ | 0.4mg/ml | 88.54％ |
Experimental results show that the Chinese herbal medicine compositions of examples 1, 2 and 3 have better antioxidant capacity for scavenging free radicals at the concentrations of 0.08mg/ml and 0.4mg/ml in vitro, and the Chinese herbal medicine composition of example 2 has the best effect.
Example 6: hyaluronidase inhibition assay
The degradation reaction of hyaluronic acid depends on the activity of hyaluronidase, and the degradation product of macromolecular hyaluronic acid can cause inflammatory reaction of skin, so that the inhibition of the activity of hyaluronidase is used as an index of the anti-inflammatory reaction of substances, and the higher the inhibition rate of hyaluronidase represents the better anti-inflammatory activity.
Method comparison: cromolyn sodium (5mg/ml aqueous solution).
Instruments and reagents: spectrophotometer, hyaluronidase (Sigma), hyaluronic acid (cosmetic grade, provided by forrad), color developer, etc.
Sample treatment: the extracts of the examples were diluted to three concentrations of 10mg/ml, 5mg/ml and 1mg/ml, respectively, by dissolution and dilution with pH3.5buffer buffer
Selecting a 10ml test tube as a reaction tube, and placing the reaction tube in a sample tube (T) and a sample control tube (T)0) In each of which 400ul of a sample solution of the corresponding concentration, a positive control tube (C) and a negative control tube (C) were added0) Buffer solution is used for replacing; 50ul of hyaluronidase solution is added into the sample tube and the positive control tube and mixed evenly, 50ul of acetic acid buffer solution is added into the sample control tube and the negative control tube and mixed evenly (the specific loading is shown in the following table), and the mixture is bathed in water at 37 ℃ for 20 minutes.
1) 100ul of 12.5mM calcium chloride was added to each reaction tube, and the reaction tubes were bathed with water at 37 ℃ for 20 minutes.
2) 250ul of sodium hyaluronate was added to each reaction tube, and water bath was carried out at 37 ℃ for 40 minutes.
3) 100ul of 0.4N hydrochloric acid and 100ul of 0.4M sodium tetraborate are respectively added into the reaction tube, boiling water is added for 3 minutes, and the reaction tube is cooled to room temperature.
4) 3ml of a dimethylaminobenzaldehyde solution was added thereto, and the mixture was subjected to a water bath at 37 ℃ for 20 minutes.
5) Each reaction solution was transferred to a cuvette, and the optical density value (OD) was colorimetrically measured at 585nm in a spectrophotometer.
Examples the inhibition rate of hyaluronidase activity by the extract was calculated according to the following formula:
in the formula: t: a sample tube OD; t is0: the sample control tube OD; c: positive control tube OD; c0: negative controls were taken for OD.
The evaluation results of the antioxidant ability of the Chinese herbal medicine composition of the present invention are shown in Table 5.
TABLE 5
The experimental result shows that the Chinese herbal medicine composition in the embodiment has better hyaluronidase inhibition effect under three concentrations in vitro, namely shows certain anti-inflammatory activity, wherein the Chinese herbal medicine composition in the embodiment 2 has the best effect, and still has nearly 40% hyaluronidase inhibition activity under the solid content of 1 mg/ml.
Example 7: qualitative image experiment for epidermal cell in-vitro moisturizing/anti-drying damage
And (3) in-vitro cell angle, simulating a natural drying process, and photographing and recording the form of the cell at a corresponding time point through a microscope imaging system. The qualitative experiment can intuitively reflect the drying resistance and dehydration death prevention capabilities of the additive at the cellular level.
1. Experimental cells: hacat human epidermal keratinocyte cell line
2. The instrument equipment comprises: inverted microscope imaging system
3. Test samples, experimental groups:
4. the method comprises the following operation steps:
(1) after cell synchronization, the cells were seeded at a concentration of 5 × 104/well/500 ul in 24-well plates
(2) Except for the blank, the corresponding sample was added to each well and incubated for 24h
(3) At room temperature, the medium was aspirated from each well in turn and photographed (x 10 times) at the corresponding time point (after 0min, same drying time) under a microscope imaging system
The results of the experiment are shown in FIG. 1.
The results show that:
after the cells are placed indoors for an equal period of time, the cells of the blank cell group without the medicine are seriously dehydrated, obviously shrunk and deformed, the refractive index is increased, and the cells of the positive control group with 0.1 percent of sodium hyaluronate can basically maintain the normal state.
After being placed indoors for an equivalent period of time, the cells of the experimental group added with the Chinese herbal medicine composition of the invention have a slight dehydration phenomenon, a small part of the cells are denatured and shrunk, and the refractive index is increased.
After the ginseng extract is placed indoors for a period of time, the cells of the experimental group added with the ginseng extract have obvious dehydration phenomenon, most of the cells are denatured and shrunk, and the refractive index is increased.
And (4) experimental conclusion: the Chinese herbal medicine composition has certain water retention and moisturizing effects in vitro. The single medicine of ginseng is not provided with the function of water retention and moisture retention in vitro.
The Chinese herbal medicine composition prepared in examples 1 to 3 was used for the preparation of a skin external preparation. The skin external preparation is preferably a cosmetic composition such as a lotion, essence, cream, etc. The weight percentage of the Chinese herbal medicine composition in the skin external preparation is 0.001-20% (w/w). Preferably 0.008-10% (w/w), more preferably 0.01-5% (w/w), and most preferably 0.01-2% (w/w).
The following are examples of specific applications of the herbal composition of the present invention in skin external preparations, and formulations and preparation methods of these preparations. In the tables, "-" indicates no addition.
Example 8: preparation of face cream
Example 9: preparation of the emulsion
Example 10: preparation of jelly
Example 11: preparation of astringent
Example 12: preparation of essence
Example 13: preparation of facial mask
Example 14: preparation of eye cream
Example 15: preparation of an aerosol (cleaning foam)
Example 16: preparation of the spray
Example 17: preparation of shower gel
Example 18: preparation of facial cleanser
Claims (9)
1. The application of a Chinese herbal medicine composition in regulating oil secretion and/or resisting oxidation and/or inflammation and/or moisturizing for non-treatment purposes is characterized in that the Chinese herbal medicine composition is extracted from the following raw material medicines in proportion: 2-4 parts of scutellaria baicalensis, 1-3 parts of radix ophiopogonis, 1-3 parts of lophatherum gracile and 6-8 parts of ginseng.
2. The application of a Chinese herbal medicine composition in preparing a skin external agent for regulating oil secretion and/or resisting oxidation and/or inflammation and/or moisturizing is characterized in that the Chinese herbal medicine composition is extracted from the following raw material medicines in proportion: 2-4 parts of scutellaria baicalensis, 1-3 parts of radix ophiopogonis, 1-3 parts of lophatherum gracile and 6-8 parts of ginseng.
3. The use as claimed in claim 1 or 2, wherein said herbal composition is prepared by the following extraction process:
a) soaking radix Scutellariae, radix Ophiopogonis, and folium Bambusae, and extracting to obtain extract A;
b) soaking radix Ginseng, and extracting to obtain extract B;
c) combining the extract A and the extract B to obtain the Chinese herbal medicine composition.
4. The use of claim 3, wherein step a) or b) comprises soaking in a vehicle selected from the group consisting of: water, ethanol solutions, polyol solutions, and other solvents of similar polarity.
5. The use according to claim 3, wherein the extraction of step a) or b) comprises: decocting, reflux extracting, soaking or percolating.
6. The use of claim 2, wherein the herbal composition is used in an amount of 0.001 to 20 wt% based on the total weight of the external preparation for skin.
7. The use as claimed in claim 2, wherein said herbal composition is used in an amount of 0.008 to 10 wt% based on the total weight of said external preparation for skin.
8. The use of claim 2, wherein the herbal composition is used in an amount of 0.01 to 5 wt% based on the total weight of the external preparation for skin.
9. The use of claim 2, wherein the herbal composition is used in an amount of 0.01-2 wt% based on the total weight of the external preparation for skin.
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| Valentina WU."不放任自"油"做真正智慧型男——佰草集全新三大男仕系列强势推出".《海报时尚网 网址http:// www.haibao.com/article/1917654.htm》.2015,1-4. * |
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