CN105534802A - Phytoextraction essence composition with skin nourishing and hydrating effects and preparation method thereof - Google Patents
Phytoextraction essence composition with skin nourishing and hydrating effects and preparation method thereof Download PDFInfo
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- CN105534802A CN105534802A CN201610007885.7A CN201610007885A CN105534802A CN 105534802 A CN105534802 A CN 105534802A CN 201610007885 A CN201610007885 A CN 201610007885A CN 105534802 A CN105534802 A CN 105534802A
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- tremella
- radix ophiopogonis
- extraction elite
- nourishing
- soak
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- 238000002360 preparation method Methods 0.000 title claims description 11
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- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 22
- 150000001413 amino acids Chemical class 0.000 claims abstract description 10
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims abstract description 8
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- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims abstract description 8
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- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical class OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/731—Cellulose; Quaternized cellulose derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/41—Amines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/81—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions involving only carbon-to-carbon unsaturated bonds
- A61K8/8141—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides or nitriles thereof; Compositions of derivatives of such polymers
- A61K8/8147—Homopolymers or copolymers of acids; Metal or ammonium salts thereof, e.g. crotonic acid, (meth)acrylic acid; Compositions of derivatives of such polymers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Emergency Medicine (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a phytoextraction essence composition with the skin nourishing and hydrating effects. The phytoextraction essence composition with the skin nourishing and hydrating effects is prepared from a phase A comprising 0.3% of hydroxyethyl cellulose, 5.5% of propylene glycol, 5.0% of glycerinum, 0.7% of amino acid humectant, 0.15% of Carbomer, 0.1% of methylparaben, 0.05% of sodium hyaluronate and 0.15% of triethanolamine and a phase B comprising 2.0% of phytoextraction concentrated liquor and the balance deionized water. The phytoextraction concentrated liquor is prepared from, by weight, 1-50 parts of tremella extracted essence and 1-50 parts of imperial grass extracted essence. The phytoextraction essence composition with the skin nourishing and hydrating effects is good in hydrating effect and free of side effect on skin.
Description
Technical field
The present invention relates to beauty treatment fields, particularly relate to a kind of plant extraction elite composition and method of making the same with nourishing and moisturizing skin effect.
Background technology
Water is that skin protection is basic.All physiological activities of human body all be unable to do without water, and the transport of nutrient be unable to do without water, and the excretion of refuse and toxin be unable to do without water, and fat-reducing be unable to do without water, the more too busy to get away water of smooth skin.Water is very important to skin, has had water, and effect activity such as other such as whitenings, anti-ageing, antiallergic is then got twice the result with half the effort.Maintain skin normal moisture content, moisturizing is the basic function of cosmetics.Modern medicine study confirms: the cuticular skin water content of baby is 25%, thus smooth exquisiteness and high resilience; Decline with advancing age and year by year, the cuticular skin water content of adult female is down to 15%, even lower, when cuticular skin water content be down to 10% time, directly results in scaly dry skin, smegma reduces thus skin is followed the string, jaundice, obfuscation, tarnish, lax, even there is wrinkle.On just individual subjective feeling, the many meetings of overdried skin are with dry, tight sensation.There is multiple nature moisturizing factor in normal horny layer, wherein mainly aminoacid (40%), give a tongue-lashing pyrrolidone carboxylic acid (12%, lactic acid (12%), sugar (8.5%), carbamide (7%) and chlorine, sodium, potassium etc.
Existing most moisturizer is in the market all chemicals, can cause irreversible injury to skin.
Summary of the invention
Based on this, be necessary to provide a kind of moistening effect the plant extraction elite compositions with nourishing and moisturizing skin effect that is good, that have no side effect to skin.
Have a plant extraction elite compositions for nourishing and moisturizing skin effect, the one-tenth comprising following degree is grouped into:
A phase: hydroxyethyl-cellulose 0.3%, propylene glycol 5.5%, glycerol 5.0%, amino acid humectant 0.7%, carbomer 0.15%, Nepal's tortoise beetle ester 0.1%, hyaluronate sodium 0.05%, triethanolamine 0.15%;
B phase: plant extraction concentrated solution 2.0%, wherein comprises the Tremella extraction elite of 1-50 weight portion, the Radix Ophiopogonis extraction elite of 1-50 weight portion in plant extraction concentrated solution;
Surplus is deionized water.
Wherein in an embodiment, in the plant extraction concentrated solution of B phase, comprise the Tremella extraction elite of 3-20 weight portion, the Radix Ophiopogonis extraction elite of 3-20 weight portion.
Wherein in an embodiment, in the plant extraction concentrated solution of B phase, comprise the Tremella extraction elite of 8 weight portions, the Radix Ophiopogonis extraction elite of 12 weight portions.
Another object of the present invention is to provide a kind of preparation method with the plant extraction elite compositions of nourishing and moisturizing skin effect.
There is a preparation method for the plant extraction elite compositions of nourishing and moisturizing skin effect, comprise the steps:
(1) get Tremella, Radix Ophiopogonis, be placed in respectively volume fraction be 20% ~ 80% ethanol soak 2 ~ 48 hours, after filtration Tremella soak, Radix Ophiopogonis soak, the ethanol consumption soaked is 10 ~ 200 times of each raw material gross weight;
(2) Tremella soak step (1) obtained, Radix Ophiopogonis soak are concentrated into 2 ~ 5% of original weight amount respectively, obtain Tremella concentrated solution, Radix Ophiopogonis concentrated solution;
(3) Tremella concentrated solution step (2) obtained, Radix Ophiopogonis concentrated solution are respectively by macroporous resin adsorption, be the ethanol repeatedly eluting of 5 ~ 95% again by volume fraction, collect and merge eluent, be Tremella extraction elite, Radix Ophiopogonis extraction elite;
(4) proportionally get step (3) obtain Tremella extraction elite, Radix Ophiopogonis extraction elite be mixed to get B phase: plant extraction concentrated solution; Proportionally get each component of A phase: hydroxyethyl-cellulose, propylene glycol, glycerol, amino acid humectant, carbomer, Nepal's tortoise beetle ester, hyaluronate sodium, triethanolamine;
(5) under agitation, A phase and B phase are heated to respectively 80 DEG C to all dissolving mix homogeneously, then B is added in A phase under 80 DEG C of conditions, and deionized water to the percent of total adding surplus is 100%, stir, be cooled to room temperature, the plant extraction elite compositions of nourishing and moisturizing skin effect must be had.
Wherein in an embodiment, step also comprises the steps: in (1)
After filtration Tremella, Radix Ophiopogonis residue by volumetric concentration be again 20% ~ 80% soak with ethanol 2 ~ 48 hours, and merge the solution after immersion, obtain described Tremella soak, Radix Ophiopogonis soak.
Wherein in an embodiment, the volume fraction of the ethanol of step (3) is 40% ~ 95%.
The plant extraction elite compositions with nourishing and moisturizing skin effect that the present invention relates to, adopt natural plants cosmetics and do not use chemical drugs, to no skin irritation, and skin can be helped to recover normal function gradually, therefore natural plants cosmetics enjoy consumers, Be very effective.Theory of Chinese medical science thinks that the relation of body fluid and beauty treatment is very close.Body fluid is sufficient, and skin can be made flexible, full moistening, not easily aging; If deficiency of body fluid, skin can be shrivelled wrinkling, and desquamation pruritus, is easy to aging.Due to maintain improper, work or living environment, inherited genetic factors or some disease, cause body fluid to run off in a large number, make face skin lose ruddy, gloss, nonelastic, coarse dark and gloomy even sallow.
The plant extraction elite compositions with nourishing and moisturizing skin effect that the present invention relates to, according to tcm theory, from a large amount of natural plants, select with Tremella and Radix Ophiopogonis as raw material, prepare the described compositions with nourishing and moisturizing skin effect, wherein, the dry Tremella in Tongjiang, Tremellaceae plant Sichuan selected by described Tremella, and described Radix Ophiopogonis selects the Yunnan drying of draft pteridophyta to follow.Record according to " Chinese Pharmacopoeia " and document: Tremella sweet in the mouth, light, property flat, nontoxic, effect of existing spleen reinforcing appetizing, has again the effect of QI invigorating bowel relieving, nourishing YIN and moistening the lung.Can body immunity be strengthened, the tolerance of tumor patient to Radiotherapy chemotherapy can be strengthened again.Tremella is rich in natural plant colloid, and additional its has the effect of YIN nourishing, is can the good skin moistening food of long-term taking.It is ornamental both, also pharmaceutically acceptable, and herb has the usefulness of hemostasis, convergence.Among the people its Herb to be incinerated, for oral administrationly can treat various hemorrhage, and external mixed by vegetable oil, can treat various knife injury.It is said, it is still improved looks medicine simply, dresses after pulverizing with Ovum Gallus domesticus album, and face can be made bright and clean.
The plant extraction elite compositions with nourishing and moisturizing skin effect that the present invention relates to, it is the ultimate principle according to skin barrier and wetting agent, utilize animal model to carry out high flux screening, find the plant extraction elite compositions with remarkable skin nutrition moisture-keeping function.The plant extraction elite compositions with nourishing and moisturizing skin effect that the present invention relates to, verify confirmation Be very effective through human clinical, safety is good.Involved composition has feature stable in good, the without any side effects and cosmetic formulations of definite effect, safety; Compared with existing like product, having the feature of Be very effective, is a kind of novel external skin nourishing and moisturizing medicine.
Detailed description of the invention
Present embodiments provide a kind of plant extraction elite compositions with nourishing and moisturizing skin effect.
Have a plant extraction elite compositions for nourishing and moisturizing skin effect, the one-tenth comprising following degree is grouped into:
A phase: hydroxyethyl-cellulose 0.3%, propylene glycol 5.5%, glycerol 5.0%, amino acid humectant 0.7%, carbomer 0.15%, Nepal's tortoise beetle ester 0.1%, hyaluronate sodium 0.05%, triethanolamine 0.15%;
B phase: plant extraction concentrated solution 2.0%, wherein comprises the Tremella extraction elite of 1-50 weight portion, the Radix Ophiopogonis extraction elite of 1-50 weight portion in plant extraction concentrated solution;
Surplus is deionized water.
Preferably, the Tremella extraction elite of 3-20 weight portion, the Radix Ophiopogonis extraction elite of 3-20 weight portion is comprised in the plant extraction concentrated solution of B phase.
Preferably, the Tremella extraction elite of 8 weight portions, the Radix Ophiopogonis extraction elite of 12 weight portions is comprised in the plant extraction concentrated solution of B phase.
There is a preparation method for the plant extraction elite compositions of nourishing and moisturizing skin effect, comprise the steps:
(1) Tremella, Radix Ophiopogonis is got, be placed in respectively volume fraction be 20% ~ 80% ethanol soak 2 ~ 48 hours, after filtration Tremella, Radix Ophiopogonis residue by volumetric concentration be again 20% ~ 80% soak with ethanol 2 ~ 48 hours, and merge the solution after soaking, obtain described Tremella soak, Radix Ophiopogonis soak, the ethanol consumption soaked is 10 ~ 200 times of each raw material gross weight;
(2) Tremella soak step (1) obtained, Radix Ophiopogonis soak are concentrated into 2 ~ 5% of original weight amount respectively, obtain Tremella concentrated solution, Radix Ophiopogonis concentrated solution;
(3) Tremella concentrated solution step (2) obtained, Radix Ophiopogonis concentrated solution are respectively by macroporous resin adsorption, be the ethanol repeatedly eluting of 5 ~ 95% again by volume fraction, collect and merge eluent, be Tremella extraction elite, Radix Ophiopogonis extraction elite; The volume fraction of ethanol is preferably 40% ~ 95%.Macroporous resin adopts the XAD-2 type macroporous resin of rom one Haars Co., Ltd of the U.S., XAD-2 type macroporous resin is for monomer with styrene and propionic ester, adding Ethenylbenzene is cross-linking agent, and toluene, dimethylbenzene are porogen, and their polymerizations that is cross-linked with each other define porous skeleton structure.
(4) proportionally get step (3) obtain Tremella extraction elite, Radix Ophiopogonis extraction elite be mixed to get B phase: plant extraction concentrated solution; Proportionally get each component of A phase: hydroxyethyl-cellulose, propylene glycol, glycerol, amino acid humectant, carbomer, Nepal's tortoise beetle ester, hyaluronate sodium, triethanolamine;
(5) under agitation, A phase and B phase are heated to respectively 80 DEG C to all dissolving mix homogeneously, then B is added in A phase under 80 DEG C of conditions, and deionized water to the percent of total adding surplus is 100%, stir, be cooled to room temperature, the plant extraction elite compositions of nourishing and moisturizing skin effect must be had.
Embodiment 1
The present embodiment provides the preparation method of B phase plant extraction concentrated solution:
At room temperature, get Tremella and each 10 kilograms of Radix Ophiopogonis, with ethanol 200 kilograms of soak extraction of volumetric concentration 70%, soak time is 24 hours, residue soaks with the ethanol 200 kilograms of volumetric concentration 70% and extracts for 24 hours, then the solution after immersion is merged, obtain Tremella soak, Radix Ophiopogonis soak respectively.
Tremella soak, Radix Ophiopogonis soak are respectively through after 200 object strainer filterings, and concentrating under reduced pressure 20 times, obtains Tremella concentrated solution, Radix Ophiopogonis concentrated solution.
Again by macroporous resin on Tremella concentrated solution, Radix Ophiopogonis concentrated solution, with the ethanol elution of volumetric concentration 50%, the eluent of collection, obtain Tremella extraction elite, Radix Ophiopogonis extraction elite.
Embodiment 2
The present embodiment provides the preparation method of B phase plant extraction concentrated solution:
At room temperature, get 8 kilograms, Tremella, Radix Ophiopogonis 12 kilograms, with ethanol 400 kilograms of soak extraction of volumetric concentration 50%, soak time is 24 hours, residue soaks with the ethanol 400 kilograms of volumetric concentration 50% and extracts for 2 hours, then the solution after immersion is merged, obtain Tremella soak, Radix Ophiopogonis soak respectively.
Tremella soak, Radix Ophiopogonis soak are respectively through after 200 object strainer filterings, and concentrating under reduced pressure 40 times, obtains Tremella concentrated solution, Radix Ophiopogonis concentrated solution.
Again by macroporous resin on Tremella concentrated solution, Radix Ophiopogonis concentrated solution, with the ethanol elution of volumetric concentration 40%, the eluent of collection, obtain Tremella extraction elite, Radix Ophiopogonis extraction elite.
Embodiment 3
The present embodiment provides the preparation method of B phase plant extraction concentrated solution:
At room temperature, get 8 kilograms, Tremella, Radix Ophiopogonis 15 kilograms, with ethanol 300 kilograms of soak extraction of volumetric concentration 80%, soak time is 48 hours, residue soaks with the ethanol 300 kilograms of volumetric concentration 80% and extracts for 24 hours, then the solution after immersion is merged, obtain Tremella soak, Radix Ophiopogonis soak respectively.
Tremella soak, Radix Ophiopogonis soak are respectively through after 200 object strainer filterings, and concentrating under reduced pressure 25 times, obtains Tremella concentrated solution, Radix Ophiopogonis concentrated solution.
Again by macroporous resin on Tremella concentrated solution, Radix Ophiopogonis concentrated solution, with the ethanol elution of volumetric concentration 60%, the eluent of collection, obtain Tremella extraction elite, Radix Ophiopogonis extraction elite.
Embodiment 4
The present embodiment provides the preparation method of B phase plant extraction concentrated solution:
At room temperature, get 15 kilograms, Tremella, Radix Ophiopogonis 15 kilograms, with ethanol 500 kilograms of soak extraction of volumetric concentration 30%, soak time is 30 hours, residue soaks with the ethanol 500 kilograms of volumetric concentration 30% and extracts for 24 hours, then the solution after immersion is merged, obtain Tremella soak, Radix Ophiopogonis soak respectively.
Tremella soak, Radix Ophiopogonis soak are respectively through after 200 object strainer filterings, and concentrating under reduced pressure 50 times, obtains Tremella concentrated solution, Radix Ophiopogonis concentrated solution.
Again by macroporous resin on Tremella concentrated solution, Radix Ophiopogonis concentrated solution, with the ethanol elution of volumetric concentration 50%, the eluent of collection, obtain Tremella extraction elite, Radix Ophiopogonis extraction elite.
Embodiment 5
The Tremella extraction elite of 1% percentage ratio that Example 1 is obtained, the Radix Ophiopogonis extraction elite of 1% percentage ratio, extract elite, Radix Ophiopogonis extraction elite be mixed to get B phase by Tremella:
The component of following degree is got by table 1, hydroxyethyl-cellulose 0.3%, propylene glycol 5.5%, glycerol 5.0%, amino acid humectant 0.7%, carbomer 0.15%, Nepal's tortoise beetle ester 0.1%, hyaluronate sodium 0.05%, triethanolamine 0.15%, be mixed to get A phase.
Under agitation, A phase and B phase are heated to respectively 80 DEG C to all dissolving mix homogeneously, then B is added in A phase under 80 DEG C of conditions, and add the deionized water of surplus 85.05%, stir, be cooled to room temperature, the plant extraction elite compositions of nourishing and moisturizing skin effect must be had.
Table 1
Embodiment 6
12 volunteers (age 24-50) carry out binocular test
White by the cream with the plant extraction elite compositions of nourishing and moisturizing skin effect that coating embodiment 5 is obtained on an order wherein, another order applies placebo frost equally.Twice daily, dosage 2mg/c ㎡.
Research shows, the plant extraction elite compositions with nourishing and moisturizing skin effect improves short-term and long-term moisturizing degree.After 3 hours and 8 days, the position capacitance of plant extraction elite moisturiser in the experimenter of 66.7%, is used significantly to increase.Illustrate that the plant extraction elite compositions with nourishing and moisturizing skin effect helps to improve skin moisture-keeping degree.
Research shows, high TEWL value shows the decline of skin barrier function.For the experimenter of 66.7%, position is used relative to placebo frost, the cream frost with the plant extraction elite compositions of nourishing and moisturizing skin effect uses position TEWL value to decline, show that skin barrier function promotes, illustrate that the plant extraction elite compositions with nourishing and moisturizing skin effect helps to promote skin barrier function.The application with the plant extraction elite compositions of nourishing and moisturizing skin effect makes cuticular thickness decrease.There is the plant extraction elite compositions of nourishing and moisturizing skin effect, synthesizing by activating the key molecule relevant with epidermis hydration, help skin to maintain its lock outlet capacity.Improve silk polyprotein, generate a large amount of aminoacid; By derivatives, obtain nature moisturizing factor.By good hydration levels, provide elasticity and the integrity of epidermal barrier, improve GAGs, improve water storage function.IN-VIVO result shows, plant extraction elite composition for improved moisture-retaining capacity, can moisturizing (electric capacity), has skin barrier function (TEWL).
Embodiment 7
There is the clinical experimental study report of the plant extraction elite compositions of nourishing and moisturizing skin effect: in following clinical trial, the compositions of drug extract used is the plant extraction elite compositions with nourishing and moisturizing skin effect of embodiment 5.
1. materials and methods
The selection of 1.1 tested objects
Altogether include 30 female volunteers of 24 ~ 42 years old in, inclusive criteria: women, live in this locality more than 5 years; Based on office work, without high dose Exposure to Sunlight; Measuring point is without dermatosis or with cutaneous systemic disease; Skin
Unglazed allergies, without cosmetic allergic contact dermatitis history; To research purpose and content informed consent, person can not followed up a case by regular visits on request.Exclusion standard: known to the composition allergy sufferers in tested products; Psychotic; Be in person in period of pregnancy, age of sucking or later six months of giving a birth; Have
Serious hepatorenal disease, autoimmune disease (as systemic lupus erythematosus (sle), rheumatoid arthritis), HIV person: in nearly 12 months, be diagnosed as cutaneous tumor and curer; Using and/or oral in 14 days or the external medicine (glucocorticoid, immunosuppressant antiallergic agent) of test forbidding before the test; Using or testing and using other like products person in first 1 month.
1.3 test instrunments adopt SebumeterSN810 sebum measuring instrument (German CourageKhazaka company)
Measure skin surface sebum content, adopt ComeunterCM820 moisture of skin measuring instrument (German CourageKhazaka company) to measure skin moisture content.The unit of sebum level and skin moisture content is au (arbitraryunit).
1.4 test conditions are without direct sunlight, calm, operating ambient temperature is 22 ~ 24 DEG C, relative humidity is 50% ~ 60%, and confirm before measuring that volunteer did not make to apply some make up the same day, volunteer cleans facial tranquillization 20 minutes, Pi Wen and indoor temperature is made to reach consistent, lie on a bed with posture easily, before tested position Olay moistens fancy soap cleaning deeply and clear-headed after 1h, carry out the detection of sebum, moisture successively.
1.5 smearing method tested objects use 30d continuously, and after washing one's face before sleeping in every morning, evening, purple face smears the essence with facial nourishing and moisturizing effect, and applying amount is to be slightly shown in smear layer.
Test position application sebum secretion tester carries out sebum secretion mensuration to patient's bilateral wing of nose position; Application water content of stratum corneum tester carries out water content of stratum corneum mensuration to patient's bilateral malar reduction highest portion position, and each position surveys 10 times, averages after removing peak and minimum.
1.6 safety evaluatios carry out untoward reaction evaluation when further consultation to experimenter, the former symptom of inquiry experimenter's target site
Whether increase the weight of or occur new skin lesion, as symptoms such as pruritus, drying, desquamation, twinge, redness, desquamations and whether hinder daily life.Severe: untoward reaction hinders daily routines, experimenter's subjective symptoms is remarkable, is impatient at, and needs to stop using; Moderate: untoward reaction hinders daily life, experimenter's subjective symptoms is obvious, but can stand, tested products of need not stopping using; Slight: can react by Intolerance, experimenter accidentally experiences.To the experimenter occurring untoward reaction, doctor carries out patch test to it. to determine the cause effect relation of untoward reaction and test products.Responsible doctor thinks that experimenter is not suitable for continuing test, or experimenter requires to stop, and namely stops the test of this experimenter, and records stop reason and time.
1.7. statistical procedures adopts SSPS11.5 software to carry out paired t-test before and after self.
2. result
2.130 volunteers use front and back skin sebum content and water content of stratum corneum comparison sheet 2 to show, 30 volunteers use pharmaceutical composition 30d skin sebum content significance of the present invention to raise (P < 0.05), and cuticular water content also obviously raises (P < 0.01).
Before and after table 2 uses, 30 volunteer's skin sebum content and water content of stratum corneum compare (± s, au)
| Before using | After using | |
| Sebum | 12.72±2.58 | 17.51±3.28 · |
| Cuticlar water content | 42.75±11.38 | 56.34±18.95 ·· |
*P＜0.05,**P＜0.01
All there is not untoward reaction before Product Safety assessment uses, come off without 1 routine volunteer.There is not the Adverse Event relevant to this test products in whole research process.
Embodiment 8
There is the skin irritation test research report of the plant extraction elite compositions of nourishing and moisturizing skin effect: by animal experiment, observe and of the present inventionly there is nourishing and moisturizing skin effect plant extraction elite compositions to the zest of rabbit skin.In following experiment, the plant extraction elite compositions with nourishing and moisturizing skin effect that the compositions embodiment of plant extraction elite compositions extract used 4 is obtained.
1 animal
16 regular grade adult healthy new zealand rabbits, male and female half and half, 2.5kg ~ 3.Skg, buys in Guangdong Medical Lab Animal Center, and laboratory animal production licence number is SCXK (Guangdong) 2008-0002, the animal quality certification number: Guangdong 2012A008.Raise in animal F4 room, general area, new southern Drug safety assessment center (the experimental animal room quality certification number: 0060590).Duration of test, room temperature 20 DEG C ~ 22 DEG C, humidity 40% ~ 67%, illumination 12h/12h round the clock light and shade replaces.The pharmaceutical composition that the pharmaceutical composition used in this experiment is the embodiment of the present invention 1, concentration is 1.0gs crude drug/Ml.
2 methods
According to Ministry of Health of the People's Republic of China " cosmetics health specification (version in 2007) ", adopt consubstantiality self left and right sides matching type, left side is given and bare substrate (normal saline), and right side is given and the plant extraction elite compositions of 1.0g/mL.Prepare before administration: area 2.5mc × 2.5cm, not injured skin.Administration: get tested material be about 0.5mL spread upon right side skin on, left side be coated with normal saline in contrast, smear 1 every day, continuous 14d.From second day, will by hair before smearing every day.Medicinal liquid 0.5mL is soaked into the double-deck hospital gauze sheet of 2.5mc × 2.5cm, be covered in plucked skin, cover one deck template, then fix with nonirritant adhesive plaster and fixing band.Tested material is removed, by the clear residual tested material of warm water after fixing about 4h.Observed result after one hour.
3 results
Under this experiment condition, the pharmaceutical composition smearing 1.0g/mL continuously reacts (specifically in table 3) to rabbit no skin irritation in 14 days.
Table 31.0g/mL plant extraction elite compositions is to rabbit repeatedly irritation test result
Embodiment 9
There is the hypersensitive laboratory report of the plant extraction elite compositions of nourishing and moisturizing skin effect: by animal experiment, observe the present invention and there is the plant extraction elite compositions of nourishing and moisturizing skin effect to the sensitization of Haretley guinea pig skin.The pharmaceutical composition that the plant extraction elite compositions used in this experiment is experimental example 5 of the present invention.
1 animal
Select regular grade Haretley Cavia porcellus 50, male and female half and half, weight range 180 ~ 250g, buy in Guangdong Medical Lab Animal Center, it can the number of card be SCXK (Guangdong) 2008-0002 perhaps that laboratory animal is produced, the animal quality certification number: Guangdong 2013A008.Raise in Animal House F4 room, general area, new southern Drug safety assessment center (the experimental animal room quality certification number: 0060590.) duration of test, room temperature 20 DEG C ~ 22 DEG C, humidity 40% ~ 67%, illumination 12h/12h round the clock light and shade replaces.By number skin dyeing is carried out to Cavia porcellus, with saturated picric acid solution (yellow) dye marker units, with 2% silver nitrate solution (coffee color) dye marker tens; Separately raise by sex, each 5, cage box room Cavia porcellus, cage box is outer labelled, indicates the information such as special topic numbering, animal category, group, sex, route of administration, cage number, number of animals and desired assay commencement date and deadline.The pharmaceutical composition used in this experiment is the pharmaceutical composition of experimental example 1 of the present invention, and concentration is 1.0g crude drug/mL.
2 methods
According to country " drug registration management method ", " Chemical induced irritation, anaphylaxis and hemolytic investigative technique guideline ".Be divided into negative control group, positive controls, plant extraction elite compositions at random, wherein negative control group 10 Cavia porcelluss, all the other respectively organize 20 Cavia porcelluss.Each group of Cavia porcellus male and female half and half.Successively carry out induction sensitization and contact experiment with exciting, two test between be separated by eluting phase of 10 days.Induction exposure experimental technique is front 24h preserved skin, cut off each 2cm × 2cm region, Cavia porcellus spinal column both sides by hair.Positive control: get 2.5%2, the acetone soln 0.2g of 4-dinitro-chloro-benzene, cover with two-layer gauze (2.5em × 2.5cm) and one deck preservative film respectively, 6h is covered with non-stimulated cloth, mobile covering, remove residual given the test agent with warm water, the 7th day and the 14th day, in kind repeat once; Be coated with distilled water 0.4mL in negative control thing treated animal same area, plant extraction elite compositions group is coated with 0.5ml.Induction exposure is carried out by same step.Contact is excited to be then to latter 14 days of tested material sensitization contact (namely testing the 28th day) at last, at experimental group and negative control thing guinea pig back preserved skin place coating 0.5ml, package box is fixed, tested material is removed after 6h, observe at once, then again observe skin allergy situation in 24h, 48h, 72h.The positive controls acetone soln 0.2g of 2.5%2,4-dinitro-chloro-benzene, negative control treated animal same area is coated with distilled water 0.4ml.After sensitization, 24h and 48h observes administration local skin erythema, edema and other abnormal responses.By skin allergy standards of grading, skin erythema and edema are marked, and calculate the anaphylaxis incidence rate of each group, by skin hypersensitivity evaluation criterion anaphylaxis occurrence degree.Anaphylaxis incidence rate=(occurring number of animals/this treated animal sum of skin erythema, edema or systemic anaphylaxis in group) × 100%.
3 methods
Excite contact experiment rear 24, 48h, by not knowing that the professional of experiment content is according to " Chemical induced irritation, anaphylaxis and hemolytic investigative technique guideline " pass judgment on whether occur sensitization of skin reaction (table 4), sensitivity response scoring >=2 timesharing, judge that this animal skin anaphylaxis is positive, calculate sensitization rate (>=2 points of number of animals/these treated animal sum × 100%), and by " Chemical induced irritation, anaphylaxis and hemolytic investigative technique guideline " in sensitization of skin reaction test grade scale judge the sensitization intensity (table 4) of pharmaceutical composition of the present invention to guinea pig skin.
Table 4 sensitization of skin extent of reaction standards of grading
First calculate and put each group of integral mean value each observing time, then calculate and to observe in the time limit animal integral mean value every day every, evaluate by " Chemical induced irritation, anaphylaxis and the hemolytic investigative technique guideline " of table 5, and calculate animal average integral every day every.
Table 5 sensitization of skin extent of reaction grade scale
| Sensitization incidence rate (%) | Hypersensitive is evaluated |
| 0～10 | Weak sensitization |
| 11～30 | Slight sensitization |
| 31～60 | Moderate sensitization |
| 61～80 | Height sensitization |
| 81～100 | Extremely sensitization |
Excite rear 24h, 48h, the Cavia porcellus of negative control group, pharmaceutical composition excites area skin position all not occur erythema, edema situation, and also do not occur other abnormal responses, anaphylaxis incidence rate is 0, and anaphylaxis evaluation is showed no skin allergy.Excite rear 24h, positive controls Cavia porcellus excites area skin all to occur erythema, and anaphylaxis incidence rate is 100%, and anaphylaxis is evaluated as extremely strong sensitization.Excite rear 48h, positive controls Cavia porcellus excites area skin still all to have erythema, and anaphylaxis incidence rate is 100%, and anaphylaxis evaluation is still extremely strong sensitization.
4 conclusions
Under this experiment condition, plant extraction elite compositions to Hartley guinea pig skin carry out having no after 3 sensitization excite for 1 time skin allergy sends out should.
The plant extraction elite compositions with nourishing and moisturizing skin effect that the present invention relates to, adopt natural plants cosmetics and do not use chemical drugs, to no skin irritation, and skin can be helped to recover normal function gradually, therefore natural plants cosmetics enjoy consumers, Be very effective.Theory of Chinese medical science thinks that the relation of body fluid and beauty treatment is very close.Body fluid is sufficient, and skin can be made flexible, full moistening, not easily aging; If deficiency of body fluid, skin can be shrivelled wrinkling, and desquamation pruritus, is easy to aging.Due to maintain improper, work or living environment, inherited genetic factors or some disease, cause body fluid to run off in a large number, make face skin lose ruddy, gloss, nonelastic, coarse dark and gloomy even sallow.
The plant extraction elite compositions with nourishing and moisturizing skin effect that the present invention relates to, according to tcm theory, from a large amount of natural plants, select with Tremella and Radix Ophiopogonis as raw material, prepare the described compositions with nourishing and moisturizing skin effect, wherein, the dry Tremella in Tongjiang, Tremellaceae plant Sichuan selected by described Tremella, and described Radix Ophiopogonis selects the Yunnan drying of draft pteridophyta to follow.Record according to " Chinese Pharmacopoeia " and document: Tremella sweet in the mouth, light, property flat, nontoxic, effect of existing spleen reinforcing appetizing, has again the effect of QI invigorating bowel relieving, nourishing YIN and moistening the lung.Can body immunity be strengthened, the tolerance of tumor patient to Radiotherapy chemotherapy can be strengthened again.Tremella is rich in natural plant colloid, and additional its has the effect of YIN nourishing, is can the good skin moistening food of long-term taking.It is ornamental both, also pharmaceutically acceptable, and herb has the usefulness of hemostasis, convergence.Among the people its Herb to be incinerated, for oral administrationly can treat various hemorrhage, and external mixed by vegetable oil, can treat various knife injury.It is said, it is still improved looks medicine simply, dresses after pulverizing with Ovum Gallus domesticus album, and face can be made bright and clean.
The plant extraction elite compositions with nourishing and moisturizing skin effect that the present invention relates to, it is the ultimate principle according to skin barrier and wetting agent, utilize animal model to carry out high flux screening, find the plant extraction elite compositions with remarkable skin nutrition moisture-keeping function.The plant extraction elite compositions with nourishing and moisturizing skin effect that the present invention relates to, verify confirmation Be very effective through human clinical, safety is good.Involved composition has feature stable in good, the without any side effects and cosmetic formulations of definite effect, safety; Compared with existing like product, having the feature of Be very effective, is a kind of novel external skin nourishing and moisturizing medicine.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (6)
1. have a plant extraction elite compositions for nourishing and moisturizing skin effect, it is characterized in that, the one-tenth comprising following degree is grouped into:
A phase: hydroxyethyl-cellulose 0.3%, propylene glycol 5.5%, glycerol 5.0%, amino acid humectant 0.7%, carbomer 0.15%, Nepal's tortoise beetle ester 0.1%, hyaluronate sodium 0.05%, triethanolamine 0.15%;
B phase: plant extraction concentrated solution 2.0%, wherein comprises the Tremella extraction elite of 1-50 weight portion, the Radix Ophiopogonis extraction elite of 1-50 weight portion in plant extraction concentrated solution;
Surplus is deionized water.
2. the plant extraction elite compositions with nourishing and moisturizing skin effect according to claim 1, is characterized in that, comprises the Tremella extraction elite of 3-20 weight portion, the Radix Ophiopogonis extraction elite of 3-20 weight portion in the plant extraction concentrated solution of B phase.
3. the plant extraction elite compositions with nourishing and moisturizing skin effect according to claim 2, is characterized in that, comprises the Tremella extraction elite of 8 weight portions, the Radix Ophiopogonis extraction elite of 12 weight portions in the plant extraction concentrated solution of B phase.
4. there is a preparation method for the plant extraction elite compositions of nourishing and moisturizing skin effect, it is characterized in that, comprise the steps:
(1) get Tremella, Radix Ophiopogonis, be placed in respectively volume fraction be 20% ~ 80% ethanol soak 2 ~ 48 hours, after filtration Tremella soak, Radix Ophiopogonis soak, the ethanol consumption soaked is 10 ~ 200 times of each raw material gross weight;
(2) Tremella soak step (1) obtained, Radix Ophiopogonis soak are concentrated into 2 ~ 5% of original weight amount respectively, obtain Tremella concentrated solution, Radix Ophiopogonis concentrated solution;
(3) Tremella concentrated solution step (2) obtained, Radix Ophiopogonis concentrated solution are respectively by macroporous resin adsorption, be the ethanol repeatedly eluting of 5 ~ 95% again by volume fraction, collect and merge eluent, be Tremella extraction elite, Radix Ophiopogonis extraction elite;
(4) proportionally get step (3) obtain Tremella extraction elite, Radix Ophiopogonis extraction elite be mixed to get B phase: plant extraction concentrated solution; Proportionally get each component of A phase: hydroxyethyl-cellulose, propylene glycol, glycerol, amino acid humectant, carbomer, Nepal's tortoise beetle ester, hyaluronate sodium, triethanolamine;
(5) under agitation, A phase and B phase are heated to respectively 80 DEG C to all dissolving mix homogeneously, then B is added in A phase under 80 DEG C of conditions, and deionized water to the percent of total adding surplus is 100%, stir, be cooled to room temperature, the plant extraction elite compositions of nourishing and moisturizing skin effect must be had.
5. the preparation method with the plant extraction elite compositions of nourishing and moisturizing skin effect according to claim 4, it is characterized in that, step also comprises the steps: in (1)
After filtration Tremella, Radix Ophiopogonis residue by volumetric concentration be again 20% ~ 80% soak with ethanol 2 ~ 48 hours, and merge the solution after immersion, obtain described Tremella soak, Radix Ophiopogonis soak.
6. the preparation method with the plant extraction elite compositions of nourishing and moisturizing skin effect according to claim 4, is characterized in that, the volume fraction of the ethanol of step (3) is 40% ~ 95%.
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| CN1562994A (en) * | 2004-04-21 | 2005-01-12 | 徐州技源天然保健品有限公司 | Producing technique for extracting extract products of amentoflavone |
| CN102258442A (en) * | 2011-07-15 | 2011-11-30 | 广州市娇兰化妆品有限公司 | Compound traditional Chinese medicine extract and application thereof to whitening, moisturizing and anti-aging skin care product |
| CN103054771A (en) * | 2012-12-21 | 2013-04-24 | 黎东宇 | Ultra-elastic water-locking facial mask essence and preparation method thereof |
| CN104666236A (en) * | 2015-02-13 | 2015-06-03 | 安婕妤化妆品科技股份有限公司 | Moisturizing skincare mask |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1562994A (en) * | 2004-04-21 | 2005-01-12 | 徐州技源天然保健品有限公司 | Producing technique for extracting extract products of amentoflavone |
| CN102258442A (en) * | 2011-07-15 | 2011-11-30 | 广州市娇兰化妆品有限公司 | Compound traditional Chinese medicine extract and application thereof to whitening, moisturizing and anti-aging skin care product |
| CN103054771A (en) * | 2012-12-21 | 2013-04-24 | 黎东宇 | Ultra-elastic water-locking facial mask essence and preparation method thereof |
| CN104666236A (en) * | 2015-02-13 | 2015-06-03 | 安婕妤化妆品科技股份有限公司 | Moisturizing skincare mask |
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