CN115969759B

CN115969759B - Photoprotective plant extract compositions and uses thereof

Info

Publication number: CN115969759B
Application number: CN202310068609.1A
Authority: CN
Inventors: 招敏聪; 李璐; 田佳佳; 贺锐; 解勇
Original assignee: Plant Doctor Guangdong Biotechnology Co ltd
Current assignee: Plant Doctor Guangdong Biotechnology Co ltd
Priority date: 2023-02-06
Filing date: 2023-02-06
Publication date: 2023-12-08
Anticipated expiration: 2043-02-06
Also published as: CN115969759A

Abstract

The invention discloses a photoprotective plant extract composition and application thereof, belonging to the field of cosmetic preparations. Comprises the following components: aloe vera extract, arhat pine extract, ginger flower extract, chamomile extract, umarone, sabina japonica oil, and tea tree essential oil. The light protection plant extract composition provided by the invention is a pure natural plant source, and satisfies the preference of consumers for nature. The invention has obvious synergistic effect through the combination and the compounding of a plurality of plant extracts; on one hand, the composition has the effects of resisting allergy, relieving itching and the like and relieving adverse reactions after sunburn; on the other hand, has the effect of accelerating skin repair; targeted repair of ultraviolet-induced cell damage and alleviation of skin allergic reactions. The photoprotective plant extract composition provided by the invention can be added into various cosmetics/skin care products, and has good applicability.

Description

Photoprotective plant extract compositions and uses thereof

Technical Field

The invention belongs to the field of cosmetic preparations, and particularly relates to a photoprotection plant extract composition and application thereof.

Background

Solar radiation comprises about 5% Ultraviolet (UV) radiation, which has a wavelength in the range of 200nm to 400nm. Further ultraviolet rays can be classified into three types: 320 to 400mm (UV-A), 290 to 320nm (UV-B) and 200 to 290nm (UV-C). However, most of the UV-C radiation is absorbed by the ozone layer, so that UV-A and UV-B have Sup>A large influence on human skin.

Scientific studies have demonstrated that short exposure to UV-Sup>A and UV-B radiation can lead to redness and localized irritation of the skin, while continuous and prolonged exposure can lead to the formation of melanomSup>A and wrinkles. UV radiation has also been reported to cause serious hair damage. Therefore, there is Sup>A need to protect the human skin and other keratinous materials from the damaging effects of UV-Sup>A and UV-B radiation.

Meanwhile, exposure to ultraviolet rays with high intensity can cause serious reactions such as sunburn and the like, cause various biological effects, increase intracellular active oxygen groups, damage lipid, protein and nucleic acid in skin, and cause erythema, edema, hyperplasia, photoaging, pigmentation, DNA damage and immunosuppression of skin.

Disclosure of Invention

The invention provides a photo-protecting plant extract composition and application thereof, which play a role in repairing skin lost by ultraviolet rays through natural components extracted from plants.

In order to achieve the above purpose, the present invention adopts the following technical scheme:

a light protection plant extract composition comprises the following components in parts by mass:

in particular, the aloe vera extract is prepared by: removing epidermis from fresh aloe leaf, taking mesophyll part, crushing mesophyll, squeezing juice, centrifuging, and collecting supernatant.

In particular, the preparation method of the Podocarpus arpium is as follows: soaking the fruit support part of mature fruit of Podocarpus arpifolia in water under heating, filtering to obtain filtrate, and concentrating under reduced pressure.

Further, the preparation method of the Podocarpus arpus includes: taking the fruit support part of the mature fruit of Podocarpus arpisiformis, adding 8-10 volumes of water, heating and soaking for 2-4h at 80-95 ℃; filtering to obtain filtrate, and concentrating under reduced pressure until the residual volume is less than or equal to 20% of the filtrate volume;

the water contained 0.5V/V% acetic acid.

In particular, the preparation method of the ginger flower extract comprises the following steps: drying flowers, leaves and pseudo stems of the ginger flowers, and adding 75V/V% ethanol water solution; reflux-extracting at 60-75deg.C for at least 3 hr; concentrating, and removing solvent to obtain rhizoma Zingiberis recens flower extract;

the addition amount of the ethanol aqueous solution is 8-10 times of the total volume of flowers, leaves and pseudostems of the dried gingers.

The invention also provides application of the photoprotective plant extract composition in preparing skin care products.

The invention also provides a resident skin external agent which comprises the light protection plant extract composition.

In particular, the photoprotective plant extract composition is added in a proportion of 3-12% by weight.

In particular, the resident skin external agent further comprises auxiliary materials, wherein the auxiliary materials are selected from one or more of thickening agents, emulsifying agents, skin conditioning agents, antioxidants, moisturizers, whitening agents, pH regulators and preservatives;

the preparation form of the resident skin external agent is emulsion, gel or cream.

In the invention, the following components are added:

the aloe vera extract is used as a main component, aloe polysaccharide is contained in the extract, the effect of repairing skin loss can be achieved, and meanwhile, the contained water can be used as a solvent for dissolving other substances.

The Podocarpus arpus chinensis extract adopts a nontoxic and edible mature fruit support part, and can improve the polysaccharide content by extraction under the premise of ensuring the safety; the invention discovers that the Podocarpus arpus chinensis extract has positive auxiliary effect on skin repair.

The extract has effects of scavenging free radicals, inhibiting bacteria, promoting skin cell proliferation, and reducing oxidative damage.

The chamomile extract is a petal part of microorganism low-temperature fermentation, is mainly used as an anti-inflammatory agent and an antioxidant, and has the effects of relieving and resisting allergy.

The fresh ketone is an organic authentication natural plant emulsifier and a bacteriostatic agent which are developed by symrise and have the trade name of dragorince; the water-soluble raw material and the oil component in the present invention can be emulsified to form a stable system.

Researches show that the Australian blue cypress oil has the effects of improving and repairing inflamed skin and also has certain antibacterial and skin cell regeneration promoting effects; in addition, the blue color of the Australian blue cypress oil can properly cover the red color of damaged skin, so that the tone is biased to pink or pink and the saturation of the color is reduced.

Tea tree essential oil has antibacterial, antiinflammatory, pore astringing, and skin caring effects.

Compared with the prior art, the invention has the beneficial effects that:

(1) The light protection plant extract composition provided by the invention is a pure natural plant source, and satisfies the preference of consumers for nature.

(2) The invention has obvious synergistic effect through the combination and the compounding of a plurality of plant extracts; on one hand, the composition has the effects of resisting allergy, relieving itching and the like and relieving adverse reactions after sunburn; on the other hand, has the effect of accelerating skin repair; targeted repair of ultraviolet-induced cell damage and alleviation of skin allergic reactions.

(3) The photoprotective plant extract composition provided by the invention can be added into various cosmetics/skin care products, and has good applicability.

Detailed Description

For a better understanding of the present invention, reference will now be made to the following description of specific examples, which are included in the terminology used to describe specific embodiments of the invention and are not intended to limit the scope of the invention.

In the invention, the following components are added:

the chamomile EXTRACT is ANTHEMIS NOBILIS FLOWER EXTRACT, CAS No. 84649-86-5, EINECS No. 283-467-5.

Aloe vera refers to the Aloe vera (l.) norm. F. Plant of the genus Aloe of the family liliaceae.

Podocarpus refers to a d.don plant of genus Podocarpus macrophyllus (thunder.) of family Podocarpus; and the artificial propagation of the Arhat pine is adopted in the invention.

The gingerol is Hedychium coronarium Koen plant of genus gingerol of family Zingiberaceae.

The Australian blue cypress OIL is CALLITRIS INTRATROPICA WOOD OIL and the CAS number is 180287-43-8.

TEA TREE essential OIL, also known as melaleuca alternifolia LEAF OIL, is MELALEUCA ALTERNIFOLIA (TEA TREE) LEAF OIL; CAS number 8022-72-8 and EINECS number 617-013-8.

The experimental methods, in which specific conditions are not noted in the following examples, are generally conducted under conventional conditions or under conditions recommended by the manufacturer. Percentages and parts are by weight unless otherwise indicated.

The preparation of aloe vera extract comprises the steps of:

selecting aloe vera planted for more than five years, taking mature leaves with the length more than or equal to 40cm and the widest part more than or equal to 6 cm; cleaning, removing leaf skin, taking internal gelatinous mesophyll, stirring, crushing, squeezing, centrifuging at 4000-5000rpm for 60min, and collecting supernatant to obtain Aloe vera extract.

The preparation of the Podocarpus arpus extract comprises the following steps:

cleaning fruit support part of mature fruit of Podocarpus arpifolia, soaking in water and acetic acid under heating, filtering to obtain filtrate, and concentrating under reduced pressure to obtain Podocarpus arpus arpifolia extract; wherein the water contains 0.5V/V% acetic acid.

Wherein the water content of the extract of Arhat pine 1 is 8 times of the volume of the Arhat pine cone, heating and soaking at 80 ℃ for 4 hours; the filtrate was collected by filtration and concentrated under reduced pressure to a residual volume of 20% of the filtrate volume.

Wherein the water content of the No. 2 Podocarpus arpifolia extract is 10 times of the volume of Podocarpus arpifolia, heating and soaking at 95 ℃ for 2 hours; the filtrate was collected by filtration and concentrated under reduced pressure to a residual volume of 20% of the filtrate volume.

The preparation of the gingerol extract comprises the following steps:

(1) Taking flowers, leaves and pseudo stems of ginger flowers in or before the flowering period, cleaning, drying, and removing surface water for later use;

(2) Adding 75V/V% acid ethanol water solution, reflux extracting, concentrating;

(3) Removing solvent to obtain rhizoma Zingiberis recens flower extract.

In the step (2) of the ginger flower extract No. 1, the initial pH of the acidic ethanol aqueous solution is 3; the addition amount is 10 times of the total volume of flowers, leaves and pseudostems of the dried ginger flowers; reflux extraction is carried out at 60 ℃ for 5 hours.

In the step (2) of the ginger flower extract No. 2, the initial pH of the acidic ethanol aqueous solution is 5; the addition amount is 10 times of the total volume of flowers, leaves and pseudostems of the dried ginger flowers; the reflux extraction temperature was 75℃and the time was 3h.

In the step (2) of the ginger flower extract No. 3, the initial pH of the acidic ethanol aqueous solution is 3; the addition amount is 8 times of the total volume of flowers, leaves and pseudostems of the dried ginger flowers; the reflux extraction temperature was 75℃and the time was 3h.

Preparation of a photoprotective plant extract composition comprising the steps of:

(1) Weighing the raw materials according to the table 1;

(2) Adding Arhat pine extract, rhizoma Zingiberis recens flower extract, flos Matricariae Chamomillae extract and Xinxianone into Aloe vera extract, and mixing to obtain mixture 1;

(3) Mixing folium Platycladi oil and tea tree essential oil, adding into the mixture 1, mixing again, and sealing for storage.

TABLE 1

Component content/number	Example 1	Example 2	Example 3	Example 4	Example 5	Example 6
							Aloe vera extract	100	100	100	100	100	100
Podocarpus arpus macrophyllus extract	1.2	1.2	1.5	1.2	1.2	1.5
							Arhat pine extract number	1	1	1	2	3	4
Extract of ginger flower	1	1.2	1	1.2	1	1.2
							Ginger flower extract serial number	1	1	2	2	3	3
Extract of chamomile	3	3	3	3.5	3.5	3.5
							Xinxian ketone	0.6	0.6	0.7	0.7	0.8	0.8
Cypress leaf oil	8	8	9	9	10	10
							Tea tree essential oil	3	4	4	5	4	5

The unit in table 1 is parts by mass.

Sample stability test

1. Heat resistance test: the temperature of the incubator was adjusted to 40 ℃, three samples of each of the six prepared examples were taken and placed in transparent glass bottles, the sample loading amount was 20 ml/bottle, the bottles were placed in the incubator after being sealed, and the incubator was taken out three months later, and the incubator was returned to room temperature to observe the change in appearance.

2. Cold resistance test: the temperature of the incubator is regulated to-10 ℃, three prepared six examples are taken and arranged in transparent glass bottles, the sample loading amount is 20 ml/bottle, the sealed incubator is placed in the incubator for three months, and the incubator is taken out, returns to room temperature and observes the appearance change.

3. And (3) normal temperature test: three samples of the six prepared examples were taken and placed in transparent glass bottles, the sample loading amount was 20 ml/bottle, and after the bottles were sealed and left at room temperature for 6 months, the appearance change of the essence was observed.

No phenomena such as precipitation and precipitation are observed in the heat resistance test, the cold resistance test and the normal temperature test, and the original appearance is maintained.

Human body skin patch test

The obtained 6 groups of photoprotective plant extract compositions are referred to the human skin patch experiment in 2022 cosmetic safety technical Specification.

Skin reactions were observed as standard at 30min (after the disappearance of the indentations), 24h and 48h, respectively, and the observations were recorded, see table 2.

TABLE 2 human safety test results

Numbering device	30min	24h	48h
				Example 1	Grade 0, 30	Grade 0, 30	Grade 0, 30
Example 2	Grade 0, 30	Grade 0, 30	Grade 0, 30
				Example 3	Grade 0, 30	Grade 0, 30	Grade 0, 30
Example 4	Grade 0, 30	Grade 0, 30	Grade 0, 30
				Example 5	Grade 0, 30	Grade 0, 30	Grade 0, 30
Example 6	Class 0, 30Human body	Grade 0, 30	Grade 0, 30

Inhibition test for MMP-1 expression

Studies have shown that ultraviolet radiation of epidermal keratinocytes releases cytokines that indirectly promote MMP expression by dermal fibroblasts. MMP-1 can degrade polytype collagen gelatin and proteoglycan, which is one of the causes of skin aging symptoms such as shrinkage, inelastic and the like; the anti-aging effect of the samples prepared in the previous examples was evaluated by testing their inhibition of MMP-1.

Fibroblasts were seeded into 12-well cell culture plates, each well containing 0.75X105 cells, and starved cultured in serum-free medium for 24 hours. The starved cultured cells were rinsed with PBS and treated with uv light (40 mJ). Then, the test sample was added to the cells 2 times over 48 hours. MMP-1 isolated in the medium was measured using a kit (BIOTRAK, RPN 2610). The strength of the anti-aging effect was evaluated by calculating the expression inhibition rate of MMP-1, and the results are shown in Table 3; the calculation formula is as follows:

inhibition ratio = (a-B)/a × 100%

A, without adding test sample, MMP-1 expression level after UV irradiation

B, adding the expression quantity of MMP-1 after ultraviolet irradiation of the test sample

TABLE 3 inhibition of MMP-1 expression

Numbering device	Concentration (v/v%)	Volume (mul)	Inhibition ratio (%)
				Example 1	3	50	22.6
Example 2	3	50	22.8
				Example 3	3	50	23.1
Example 4	3	50	22.8
				Example 4	6	50	38.8
Example 4	12	50	57.2
				Example 5	3	50	23.2
Example 5	6	50	39.5
				Example 5	12	50	58.1
Example 6	3	50	22.4
				Example 6	6	50	38.0
Example 6	10	50	53.3
				Example 6	12	50	56.0

In table 3, the concentrations are those of the test samples, the remainder being physiological saline.

Synthesis promotion test of collagen III

Collagen can thicken extracellular matrix, repair damaged skin, and can be used for evaluating the ability of repair after sun.

Medium exchange was performed with DMEM medium of 0.25% fbs and 250 μm magnesium ascorbyl phosphate, and the above 4 samples to be tested were added, respectively. After three days of culture, the culture supernatant was recovered, centrifuged, and the type III collagen in the obtained supernatant was measured.

The biosynthesis ability of type III collagen in cells was evaluated by measuring the content of terminal peptide (abbreviated as P III P) of type III collagen secreted in the supernatant using "RIA-gnost P III P (P III P) measuring kit". The anti-aging effect of these samples was evaluated by the synthesis acceleration rate of type iii collagen. The calculation formula is as follows:

acceleration rate = (a-B)/B100%

Type III collagen production amount after test sample addition

Type III collagen production amount without test sample added

TABLE 4 promotion of collagen III synthesis

Numbering device	Concentration (v/v%)	Volume (mul)	Promotion rate (%)
				Example 1	3	50	30.9
Example 2	3	50	31.2
				Example 3	3	50	32.8
Example 4	3	50	31.3
				Example 4	6	50	53.2
Example 4	12	50	78.3
				Example 5	3	50	32.8
Example 5	6	50	55.8
				Example 5	12	50	82.2
Example 6	3	50	30.4
				Example 6	6	50	51.8
Example 6	10	50	72.5
				Example 6	12	50	76.2

In table 4, the concentrations are those of the test samples, the remainder being physiological saline.

And (5) evaluating the moisturizing, soothing and repairing effects of the human skin.

Purpose of experiment

According to the skin state self-evaluation and the objective data of instrument test of the volunteers before and after using the product, the efficacy of the product is comprehensively evaluated by combining subjective evaluation and objective instrument data results through statistical analysis. The moisturizing, soothing, and repairing efficacy was evaluated by screening appropriate personnel for 4 weeks on samples, by skin moisture content tester, skin moisture loss tester, skin melanin and heme test probe, facial image analyzer VISIA 7, and lactic acid stinging score.

The experimental method comprises the following steps:

[1] QB/T4256-2011 cosmetic moisturizing efficacy evaluation guide

[2] Ma Li, yimei, cheng Ying, et al, method for evaluating the suitability of facial sensitive skin cosmetics Innovative [ C ]//2017, national academy of dermatology, national institute of Western medicine, 0.

[3] European Ting, yang Xing, liang Jiayi the method for evaluating efficacy of a soothing repair product was studied [ J ]. Guangdong chemical industry.

[4] Cosmetic efficacy evaluation-scientific support for relaxation efficacy claim-Wang Huan daily chemical industry

[5] T/GDCDC 021-2022 cosmetic soothing efficacy test method

Laboratory staff

The skin of eastern asian with the face sensitivity reddening and the lactic acid stinging experiment scoring 2 minutes or more was evaluated for moisturizing, soothing and repairing effects by screening 33 eastern asian skin persons 18 to 55 years old for 4 weeks with the sample, and by skin moisture content tester, transdermal water loss tester, skin melanin and heme test probe, face image analyzer VISIA 7 and lactic acid stinging score. All volunteers were able to complete 4 weeks of use, and the final effective volunteers were 33.

Test sample

Using the sample prepared in example 6, deionized water was added to adjust to a v/v% concentration of 12 emulsion.

The application part is as follows: face part

The usage amount is as follows: half coin size, about 0.5ml.

Frequency of use and period of use: is used once in the morning and evening for 30 days continuously

The using method comprises the following steps: after cleaning the face, the emulsion is applied to the face and is massaged until the emulsion is completely absorbed

Preparation before experiments

1. A subject

Inclusion criteria:

a) Healthy volunteers 18 to 60 years old;

b) The face sensitivity is reddened and the lactic acid stinging experiment scores 2 points or more.

c) Voluntary participation and signing of informed consent;

d) The specified content can be completed according to the test requirement.

Exclusion criteria

a) Antihistamines used in the last week or immunosuppressants used in the last month;

b) Any anti-inflammatory agent is applied to the tested part in the last two months;

c) A subject suffering from a clinically unhealed inflammatory skin condition;

d) Insulin dependent diabetes mellitus patients;

e) Asthma or other chronic respiratory disease patients undergoing treatment;

f) Patients who received anti-cancer chemotherapy for nearly six months;

g) Patients with immunodeficiency or autoimmune disease;

h) Women in lactation or gestation;

i) Bilateral mastectomy and bilateral axillary lymphadenectomy;

j) Participate in other clinical laboratory researchers;

k) Highly sensitive body constitution;

1) The face has large-area marks, scratches, white spots, pigmented nevi, keloids and other skin characterizations affecting the test;

m) non-voluntary participants or those who cannot fulfill the prescribed content as required by the experiment.

2 setting of the number of human subjects to be tested

The number of volunteers is ensured to be more than 30, and the subjects mainly move indoors and are prevented from being exposed to light and high temperature and high cold environments for a long time.

3 experimental protocol determination

3.1 design of the solution

The moisturizing, soothing and repairing effects of 33 subjects 18-55 years old, the subjects suffering from facial sensitivity reddening and lactic acid stinging are evaluated by screening the subjects for 2 minutes and more in a face sensitivity reddening and lactic acid stinging experiment, using the subjects for 4 weeks, and evaluating the moisturizing, soothing and repairing effects by a skin moisture content tester, a skin water loss tester, a skin color tester, a skin melanin and heme test probe, a skin glossiness tester, a face image analyzer VISIA 7 and a lactic acid stinging test.

3.2 Experimental Environment

Laboratory temperature 21±1 ℃; relative humidity 50% ± 10%.

Experimental method

1. Test procedure

1.1 Subjective evaluation of volunteers

In the form of a questionnaire, the effect after the use of the product was self-assessed to fill out the questionnaire by the subjects at visit 0W, 2W, 4W.

2.2 Objective instrument evaluation method

The test adopts a double-blind method and a self front-back comparison method, the test period is 4 weeks, the skin data acquisition times are 3 times, and the skin data acquisition times are 0W, 2W and 4W respectively. Qualified subjects were screened as required, and upon the first visit of the volunteer, volunteer informed consent was signed, data were collected for 0 weeks, and samples were dispensed. After the volunteers wash the face with the designated face, the volunteers sit still for 20min in the test environment, and after sitting still, the skin images and data begin to be collected. Volunteers need to take samples back and give the investigator a weighing at visit 2. Subjective evaluation of volunteers was done by filling out a scoring questionnaire with the volunteers.

2 test instrument

Skin moisture content tester (Corneometer CM825, CK, germany): the moisture content of the human skin cuticle is measured by adopting a capacitance method.

Percutaneous moisture loss tester (Tewameter TM300; CK, germany): the water vapor pressure gradient at different points of the epidermis formed by the water loss of the angle layer is measured by two groups of sensors with different temperatures and humidity, and the water content evaporated through the epidermis is measured.

Skin color tester (colorimeter CL400; CK, germany): the XYZ value is obtained by using a three primary color method after correcting the reflected light quantity measured by a probe by using a special color matrix, and is calculated and converted into an Lxa.b.ITA degree value.

Skin melanin and heme test probe (Mexameter MX18, germany C & K): the higher the heme content, the enhanced vascular permeability, representing a redder skin tone; the lower the heme content, the reduced vascular permeability, representing reduced redness of the skin.

Skin gloss tester (Glossymeter GL200; CK, germany): skin surface gloss is reflected by direct and diffuse reflection of light impinging on the skin surface. A parallel beam of white light generated by an LED at the tip of the probe is directed at the skin surface at an angle of 60 after passing through a planar mirror, and a portion of the light is directed at the same angle after being reflected directly through another planar mirror to a receiving transducer. Another part of the light is scattered by the skin surface and received by a sensor positioned in the vertical direction of the skin.

3 evaluation of skin observations

With the help of a facial image analyzer VISIA 7 (Canfield, U.S.A.), the instrument uses 5 different light sources to take facial images, and can visually observe the change of facial skin states.

4 test index

Skin moisture content (MMV value): MMV values characterize skin moisture, with greater MMV values indicating higher stratum corneum moisture content.

Percutaneous moisture loss (TEWL value): TEWL values are important parameters for evaluating skin barrier function, with smaller values leading to less skin loss of water through the skin.

Skin redness (a value): the lower the a value, the lower the redness of the skin.

Skin heme content (EI value): the higher the heme content, the enhanced vascular permeability, representing a redder skin tone; the lower the heme content, the reduced vascular permeability, representing reduced redness of the skin.

Skin gloss (DSC value): the larger the DSC value, the better the skin gloss.

Lactic acid stinging score: the degree of stimulation of 10% lactic acid to the subject is shown, and the smaller the value, the lower the degree of feeling of the subject to lactic acid stimulation.

Evaluation of results

Positive result judgment: before and after the product is used, the detection index has a significant difference (forward direction) from the initial value, which indicates that the product has the effect of improving the index.

Negative result judgment: before and after the product is used, the detection index and the initial value have no significant difference (forward direction), which indicates that the product has no effect of improving the index.

6 data analysis

6.1 calculation formula

Average value ofWhere x = individual parameter measurement

6.2 statistical analysis method

The data were analyzed using SPSS 19.0 data analysis software. And (3) carrying out normal distribution and variance alignment test on each parameter before statistical analysis, and carrying out paired t-test analysis when the data shows normal distribution. When the data exhibits a non-normal distribution, a pairing rank and test analysis is performed. The statistical method uses a two-tailed test with a test level of α=0.05.

Results and analysis

Subjective evaluation of skin volunteers

Table 5 subjective evaluation results of volunteers

Note that: "*": p <0.05, representing significant differences, "x": p <0.01, ", x": p <0.001, representing a very significant difference.

2 evaluation of skin Objective instruments

2.1 skin moisture MMV value results

TABLE 6 skin moisture MMV value mean results

n＝33	0W	2W	4W
				Mean value of	53.13±10.68	57.95±10.41	59.67±12.96
Difference from the initial value (delta)	/	4.82	6.54
				Rate of change	/	9.08％**	12.31％**

Note that: compare with initial value 0W, "+": p <0.05, representing significant differences, "x": p <0.01, ", x": p <0.001, representing a very significant difference.

2.2 results of the percutaneous Water loss TEWL values of skin

TABLE 7 mean results of skin transcutaneous Water loss TEWL values

n＝33	0W	2W	4W
				Mean value of	14.22±4.81	13.32±4.77	11.86±3.69
Difference from the initial value (delta)	/	-0.89	-2.36
				Rate of change	/	-6.28％	-16.58％***

2.3 skin redness a value results

TABLE 8 skin redness a value mean results

n＝33	0W	2W	4W
				Mean value of	12.26±1.76	11.59±1.77	11.22±1.87
Difference from the initial value (delta)	/	-0.67	-1.04
				Rate of change	/	-5.46％*	-8.47％***

2.4 results of skin heme EI values

TABLE 9 skin erythema EI value results

n＝33	0W	2W	4W
				Mean value of	278.73±79.28	275.37±70.88	259.18±72.50
Difference from the initial value (delta)	/	-3.36	-19.55
				Rate of change	/	-1.21％	-7.01％**

2.5 results of skin gloss DSC values

TABLE 10 skin gloss DSC values results

n＝33	0W	2W	4W
				Mean value of	4.79±1.51	6.35±1.71	6.17±1.51
Difference from the initial value (delta)	/	1.56	1.39
				Rate of change	/	32.70％***	29.00％***

2.6 lactic acid stimulation scoring results

TABLE 11 mean results of lactic acid stimulation scores

n＝30	0W	4W
			Mean value of	4.15±2.86	2.88±2.13
Difference from the initial value (delta)	/	-1.27
			Rate of change	/	-30.66％％***

Tables 12 to 17 below are corresponding raw data records

Table 12 skin moisture MMV data for volunteers

TABLE 13 data on the percutaneous Water loss TEWL values of the skin of volunteers

Table 14 skin redness a value data for volunteers

TABLE 15 skin erythema EI value data for volunteers

Table 16 volunteer skin gloss DSC value data

Table 17 raw data for lactic acid stinging score of volunteers

/>

Note that: compare with initial value 0W, "+": p <0.05, representing significant differences, "x": p <0.01, ", x": p <0.001, representing very significant differences, "-": p > 0.05, indicating no significant difference.

While specific embodiments of the invention have been described above, it will be appreciated by those skilled in the art that this is by way of example only, and the scope of the invention is defined by the appended claims. Various changes and modifications to these embodiments may be made by those skilled in the art without departing from the principles and spirit of the invention, but such changes and modifications fall within the scope of the invention.

Claims

1. A plant extract composition for repairing cell damage caused by ultraviolet rays and alleviating skin allergy, characterized by comprising the following components in parts by mass:

aloe vera extract 100

Arhat pine extract 1.2-1.5

1-1.2 parts of ginger flower extract

3-3.5% of chamomile extract

0.6 to 0.8 of Xinxian ketone

Australian blue cypress oil 8-10

Tea tree essential oil 3-5

The preparation method of the aloe vera extract comprises the following steps: removing epidermis from fresh aloe leaf, taking mesophyll part, crushing mesophyll, squeezing juice, centrifuging, and collecting supernatant;

the preparation method of the Podocarpus arpus extract comprises the following steps: taking the fruit support part of the mature fruit of Podocarpus arpisiformis, adding 8-10 volumes of water, heating and soaking for 2-4h at 80-95 ℃; filtering to obtain filtrate, and concentrating under reduced pressure until the residual volume is less than or equal to 20% of the filtrate volume; the water contains 0.5V/V% acetic acid;

the preparation method of the ginger flower extract comprises the following steps: drying flowers, leaves and pseudo stems of rhizoma Zingiberis recens flower, and adding 75V/V% acidic ethanol water solution; reflux-extracting at 60-75deg.C for at least 3 hr; concentrating, and removing solvent to obtain rhizoma Zingiberis recens flower extract; the addition amount of the acidic ethanol aqueous solution is 8-10 times of the total volume of flowers, leaves and pseudostems of the dried gingers; the pH of the acidic ethanol aqueous solution is 3-5.

2. Use of the plant extract composition of claim 1 for the preparation of skin care products.

3. A leave-on skin external preparation comprising the plant extract composition according to claim 1.

4. The resident skin external preparation according to claim 3, wherein the plant extract composition is added in a proportion of 3 to 12wt%.

5. The resident skin external agent according to claim 3, further comprising an auxiliary selected from one or more of a thickener, an emulsifier, an antioxidant, a humectant, a whitening agent, a pH adjuster, and a preservative;