CN112972314B - Eye repair composition and preparation method and application thereof - Google Patents
Eye repair composition and preparation method and application thereof Download PDFInfo
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- CN112972314B CN112972314B CN202110350057.4A CN202110350057A CN112972314B CN 112972314 B CN112972314 B CN 112972314B CN 202110350057 A CN202110350057 A CN 202110350057A CN 112972314 B CN112972314 B CN 112972314B
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/9789—Magnoliopsida [dicotyledons]
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- A61Q1/14—Preparations for removing make-up
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Landscapes
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- Veterinary Medicine (AREA)
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Abstract
The invention discloses an eye repair composition, a preparation method and application thereof, wherein the eye repair composition comprises the following components: 0.001-0.02 part of acetyl tetrapeptide-5, 1-6 parts of whitening plant extract composition, and 1-6 parts of lentinus edodes mycelium extract, wherein the parts are calculated by weight. The eye repair composition prepared by the invention is matched with peptides, plant extracts and mushroom mycelium extracts, is easy to be absorbed by skin, and can realize synergistic effect on the premise of fully playing respective roles, thereby achieving the technical effects of quickly and efficiently fading dark circles, relieving eye edema, reducing eye bags and improving the elasticity of skin around eyes. The eye repair composition prepared by the invention is mild, has no stimulation, is safe and has no side effect, and the preparation method is simple.
Description
Technical Field
The invention belongs to the technical field of daily cosmetics, and relates to an eye repair composition, a preparation method and application thereof.
Background
The eyes are the focus of attention of a person, can intuitively embody the age and the mental state of the person, and are important for improving the external image and the whole air quality and showing beauty. The eye skin is the thinnest skin of a human body, sweat glands and sebaceous glands of the skin around the eyes are rarely distributed, elastic fibers and collagen structures are lacking in dermis layers, the distribution density of nerve fibers and capillaries of the skin around the eyes is high, the working load of the eyes is heavy, and the like, and the problems of eye pockets, dark circles, wrinkles and the like are caused due to the sensitivity and weakness of the eye skin, easiness in drying, accelerated aging and the like of the eye skin based on the characteristics of the eye skin and the change of modern life modes such as pollution, illumination, pressure, night stay, unhealthy diet, smoking and the like.
The eye care products on the market at present mainly have the following defects: (1) the general product can not be absorbed well by skin, has limited effect, and can not penetrate into dermis to fundamentally improve eye health; (2) some products have single efficacy, or moisturize, or reduce eye bags, or fade dark circles, or improve wrinkles, and various effects cannot be considered, so that the products capable of improving the eye problem in all directions are deficient; (3) although some products can take effect quickly, after a period of use, side effects are easy to generate, or new problems such as fat particles are brought, so that the eye burden is increased; (4) some products have long onset period, need to be used for a long time, and can cause the rebound of the problems of eye bags, dark circles, wrinkles and the like after being stopped; (5) some products even have irritation, allergy and other phenomena after being used, and the safety is to be considered. Therefore, there is a need to develop an eye repair composition that addresses the problem of eyes in an all-around manner that is gentle, safe, fast onset of action, high efficacy, and no rebound effect.
Disclosure of Invention
The invention aims to overcome the problems in the prior art and provide an eye repair composition matched with peptides, plant extracts and mushroom mycelium extracts and a preparation method thereof. The eye repair composition is easy to be absorbed by skin, and the components can be synergistic under the premise of fully playing respective roles, so that the technical effects of quickly and efficiently fading dark circles, relieving eye edema, reducing eye bags and improving skin elasticity around eyes are achieved. In addition, the preparation method of the eye repair composition is simple, mild, free of irritation, safe and free of side effects, and can provide better health care for the eye skin.
In order to achieve the technical purpose and the technical effect, the invention is realized by the following technical scheme:
an eye repair composition comprising the following components: 0.001-0.02 part of acetyl tetrapeptide-5, 1-6 parts of whitening plant extract composition, and 1-6 parts of lentinus edodes mycelium extract, wherein the parts are calculated by weight.
Preferably, the composition comprises the following components: 0.002-0.01 part of acetyl tetrapeptide-5, 2-5 parts of whitening plant extract composition, 2-5 parts of lentinus edodes mycelium extract, and the parts are calculated by weight.
Preferably, the composition comprises the following components: 0.005 part of acetyl tetrapeptide-5, 4 parts of whitening plant extract composition, 4 parts of lentinus edodes mycelium extract, and the parts are calculated by weight.
Preferably, the whitening plant extract is composed of paeonia lactiflora root extract and reed extract in a weight ratio of 1:1.
The invention also provides a preparation method of the eye repair composition, which is characterized by comprising the step of uniformly mixing all components of the eye repair composition, namely acetyl tetrapeptide-5, the whitening plant extract composition and the lentinus edodes mycelium extract.
In certain embodiments, the method comprises:
the acetyl tetrapeptide-5 was pre-dispersed uniformly with 50% glycerol.
In certain embodiments, the method comprises:
the whitening plant extract composition is obtained through the following preparation steps: (1) Sun-drying radix paeoniae alba and reed, crushing, weighing according to the required parts, and uniformly mixing; (2) Adding 25-40 times of deionized water, extracting at 75-95deg.C for 2-4 hr for 2-3 times, and mixing extractive solutions; (3) Cooling the extract obtained in the step (2) to 30 ℃ or below, filtering with 100-200 mesh gauze, filtering twice, collecting filtrate to obtain primary filtrate, and naturally standing the primary filtrate for 24 hours; (4) Centrifuging the solution after standing in the step (3) for 0.5-1 hour at a rotating speed of 2000-10000rpm, filtering with a filter membrane with a pore diameter of 0.1-1.0 μm, and collecting filtrate to obtain secondary filtrate; (5) Concentrating the secondary filtrate obtained in the step (4) under reduced pressure, adding polyol, and uniformly mixing; (6) Filtering the solution obtained in the step (5) with a filter membrane with the pore diameter of 0.01-0.1 μm, and collecting filtrate to obtain the whitening plant extract composition.
Preferably, the weight of the secondary filtrate in the step (5) and the concentrated solution is 1/4-1/2 of the original weight.
Preferably, the weight of the added polyalcohol is 1-10 times of that of the concentrated solution, and the polyalcohol is one or more of glycerol, 1, 3-butanediol, 1, 2-pentanediol, 1, 3-propanediol and dipropylene glycol.
According to the preparation method, the eye repair composition can be obtained. The eye repair composition can be applied to cosmetics such as eye cream, eye gel, eye milk, eye essence, eye mask, eye patch, eye gel, eye makeup removing product and the like.
In the invention, the acetyl tetrapeptide-5 has mild skin-friendly and low irritation, and can be used for a long time as a humectant, a skin conditioner and the like. It can lighten dark circles, alleviate eye edema, reduce eye bags, improve skin elasticity and smoothness due to the following two aspects: firstly, acetyl tetrapeptide-5 can inhibit the activity of Angiotensin Converting Enzyme (ACE), relax capillary tension so as to promote blood circulation of eye skin, degrade bilirubin, lighten black eye, reduce capillary permeability, inhibit water in capillary vessels around eyes from flowing into skin around eyes, realize drainage efficacy, and effectively relieve edema type eye bags in 15 days; second, acetyl tetrapeptide-5 can inhibit glycosylation, reduce protein crosslinking damage caused by advanced glycosylation end products AGEs, reduce superoxide dismutase SOD inactivation caused by glycosylation, thereby maintaining normal activity of collagen and elastin, enhancing the ability of scavenging free radicals, relieving eye bags formed by saccharification and accumulation of collagen, improving skin elasticity, relieving eye wrinkles, and delaying skin aging.
According to the invention, the whitening plant extract composition can remove free radicals and inflammatory factors, and can improve pigmentation, even skin color and enable skin to be white and transparent through the ways of enhancing the resistance of skin to external stimulus, inhibiting tyrosinase activity, promoting melanin metabolism and the like.
In the invention, the lentinus edodes mycelium extract is obtained by fermenting lentinus edodes mycelium, contains active ingredients such as lentinan, small molecular polypeptides, various amino acids and the like, and has the effects of resisting oxidation, whitening, resisting ultraviolet and the like. Toxicology studies on the mushroom mycelium extract show that the mushroom mycelium extract is nontoxic and nonirritating and can be safely used in cosmetics.
The beneficial effects of the invention are as follows:
the acetyl tetrapeptide-5 selected by the invention can improve the eye bag problem in a targeted way, and can lighten dark circles and improve the skin elasticity; the whitening effect of the whitening plant extract composition enables the black eye removing effect of the eye repairing composition to be better improved; the shiitake mushroom mycelium extract has synergistic effect on improving the elasticity of the eye bags, dark circles and skin by playing the effects of antioxidation, whitening, ultraviolet resistance and the like. The eye repair composition is matched with three components, so that the problem of single eye can be solved, and more importantly, the tired state of eyes is improved on the whole, and the eyes are healed.
The eye repair composition prepared by the invention is easy to be absorbed by skin, and the components can be synergistically enhanced on the premise of fully playing respective roles, so that the technical effects of quickly and efficiently fading dark circles, relieving eye edema, reducing eye bags and improving the elasticity of skin around eyes are achieved. In addition, the preparation method of the eye repair composition is simple, mild, free of irritation, safe and free of side effects, and can provide better health care for the eye skin.
Drawings
FIG. 1 is a graph showing the measurement of the pouch volume in example 7.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
An eye repair composition comprising the following components: 0.001-0.02 part of acetyl tetrapeptide-5, 1-6 parts of whitening plant extract composition, and 1-6 parts of lentinus edodes mycelium extract, wherein the parts are calculated by weight.
Preferably, the composition comprises the following components: 0.002-0.01 part of acetyl tetrapeptide-5, 2-5 parts of whitening plant extract composition, 2-5 parts of lentinus edodes mycelium extract, and the parts are calculated by weight.
Preferably, the composition comprises the following components: 0.005 part of acetyl tetrapeptide-5, 4 parts of whitening plant extract composition, 4 parts of lentinus edodes mycelium extract, and the parts are calculated by weight.
In a specific implementation, the whitening plant extract composition consists of paeonia lactiflora root extract and reed extract in a weight ratio of 1:1.
Preferably, the acetyltetrapeptide-5 is pre-dispersed uniformly with 50% glycerol.
The preparation method of the whitening plant extract composition comprises the following steps: (1) Sun-drying the required plant parts, crushing, weighing according to the required parts, and uniformly mixing; (2) Adding 25-40 times of deionized water, extracting at 75-95deg.C for 2-4 hr for 2-3 times, and mixing extractive solutions; (3) Cooling the extract obtained in the step (2) to 30 ℃ or below, filtering with 100-200 mesh gauze, filtering twice, collecting filtrate to obtain primary filtrate, and naturally standing the primary filtrate for 24 hours; (4) Centrifuging the solution after standing in the step (3) for 0.5-1 hour at a rotating speed of 2000-10000rpm, filtering with a filter membrane with a pore diameter of 0.1-1.0 μm, and collecting filtrate to obtain secondary filtrate; (5) Concentrating the secondary filtrate obtained in the step (4) under reduced pressure, adding polyol, and uniformly mixing; (6) Filtering the solution obtained in the step (5) with a filter membrane with the pore diameter of 0.01-0.1 μm, and collecting filtrate to obtain the whitening plant extract composition.
Preferably, the weight of the concentrated solution is 1/4-1/2 of the original weight of the secondary filtrate in the step (5), the weight of the added polyalcohol is 1-10 times of the weight of the concentrated solution, and the polyalcohol is one or more of glycerol, 1, 3-butanediol, 1, 2-pentanediol, 1, 3-propanediol and dipropylene glycol.
The preparation method of the eye repair composition comprises the following steps: mixing acetyl tetrapeptide-5, whitening plant extract composition, and Lentinus Edodes mycelium extract.
The eye repair compositions of examples 1 to 12 and comparative examples 1 to 3 were prepared according to the above-described preparation methods of the whitening plant extract composition and the eye repair composition, respectively. Wherein the compositions and parts of the components in examples 1 to 12 and comparative examples 1 to 3 are shown in Table 1. The whitening plant extract composition comprises paeonia lactiflora root extract and reed extract in a weight ratio of 1:1.
TABLE 1
Experimental example 1: cytotoxicity test
MTT is a yellow powdered chemical reagent widely used in cytotoxicity or cell proliferation detection, and the detection principle is that succinic dehydrogenase in mitochondria of living cells can reduce MTT into water-insoluble blue-violet crystalline formazan and deposit the blue-violet crystalline formazan in cells, and dead cells have no function. Dimethyl sulfoxide (DMSO) can solubilize formazan in cells, and determine the number of living cells based on the measured absorbance values, as follows:
1X 10 per well in 96-well plates 4 Density of individual cells 100 μl each of DMEM medium containing 10% bovine serum and human immortalized epidermal cells (HaCaT) were inoculated, cultured for 24 hours and changed to serum-free medium; the eye repair compositions prepared in examples 1 to 12 and comparative examples 1 to 3 were added to serum-free medium, respectively, and cultured for 24 hours after treatment; the medium was removed, treated with 20. Mu.L of MTT solution (phosphate buffer as solvent at a concentration of 5 mg/ml) and incubated for 4 hours; adding 150 mu L of DMSO into the MTT solution-removed cells, and reacting the cells for 4 hours in a water bath at 37 ℃ until the crystalline formazan is completely dissolved; the absorbance at 570nm is measured, for example, according toCell viability was calculated by the following formula.
Cell viability (%) = sample absorbance value/control absorbance mean x 100%
The control group was tested without the addition of sample. Cytotoxicity related results table 2:
TABLE 2 cell viability
Experimental example 2: inhibition tyrosinase activity assay
The phosphate buffer solution having pH of 6.8, the eye repair compositions prepared in examples 1 to 12 and comparative examples 1 to 3, and the tyrosine solution were sequentially added to the test tube as shown in Table 3, water-bath was performed at 35℃for 10 minutes, then tyrosinase solution was added, uniformly mixed, incubated at 35℃for 30 minutes, rapidly transferred to the cuvette, and absorbance was measured at 475 nm. The experimental group, the negative control and the positive control are respectively zeroed by a blank control 1, a blank control 2 and a blank control 3.
The concentration of the eye repair composition and the arbutin solution used in the experiment is 1mg/mL.
Tyrosinase inhibition rate = [ (a-B)/a ] ×100%, a is absorbance value of negative control, and B is absorbance value of experimental group or positive control.
TABLE 3 Table 3
The inhibition rates of tyrosinase for examples 1-12 and comparative examples 1-3 are shown in Table 4.
TABLE 4 tyrosinase inhibition rate
From the results of table 4, the following conclusions can be drawn: (1) the eye repair compositions of examples 1-12 and comparative examples 1-3 both inhibit tyrosinase activity; (2) the results of comparative examples 2 to 5 show that the effect of example 5 is not significantly improved compared with that of example 4, and the effects of examples 3 and 4 are close, so that the addition amount of the lentinus edodes mycelium extract in the eye repair composition is preferably 4 parts or 5 parts; (3) the results of comparative examples 6 to 9 show that the effect of example 9 is not significantly improved compared with that of example 8, and the effects of examples 7 and 8 are close, so that the addition amount of the whitening plant extract composition in the eye repair composition is preferably 4 parts or 5 parts; (4) as a result of comparison of examples 3, 6, 10 and 11, it was found that the effect of example 11 was not significantly improved as compared with example 10, and that both examples 6 and 10 could achieve an effect equivalent to arbutin, so that the addition amount of acetyl tetrapeptide-5 in the eye-repairing composition was preferably 0.005 part or 0.01 part. In view of the above, the amount of acetyl tetrapeptide-5 added in the eye-repair composition is preferably 0.005 parts, and the amounts of the whitening plant extract composition and the shiitake mushroom mycelium extract are both preferably 4 parts, in combination with the cost, the stability of the formulation, the irritation, and the presence of the ingredients, and the like, example 7 is preferred in the present invention.
Experimental example 3: DPPH radical scavenging test
2.0mL of a DPPH solution (0.2 mmol/L) of 50% ethanol was precisely removed, 2.0mL of the test solution was added, the mixture was sufficiently shaken and reacted for 30 minutes, and the absorbance was measured at 517nm to use vitamin C as a positive control. The clearance of DPPH free radical from each test solution was calculated according to the following formula:
DPPH radical clearance (%) = [1- (a) i -A j )/A 0 ]×100％，A i To add the absorbance of the test solution after reaction, A j For the self absorbance of the test liquid, A 0 The absorbance of the DPPH solution without the test solution.
TABLE 5 DPPH radical scavenging Rate
Conclusion of experiment: as is clear from the results of Table 5, the eye-repair compositions of examples 1 to 12 and comparative examples 1 to 3 each have the ability to scavenge DPPH radicals, and the experimental results of inhibiting tyrosinase activity by the compositions of each example in the above experimental example 2 are combined, and the present invention is preferably in example 7.
Experimental example 4: preparation of eye essence emulsion
The present invention also prepares a sample of an eye cream comprising the eye repair composition prepared in example 7 above, the composition and parts of the components of which are shown in table 6.
The preparation method comprises the following steps: a. mixing water, chelating agent and humectant, heating to 70-80deg.C, dissolving to obtain phase A, and keeping the temperature for use; b. mixing the emollient and the emulsifier, heating to 70-80 ℃ to obtain a C phase, and preserving heat for later use; c. mixing the preservative and the eye repair composition, and uniformly stirring to obtain a D phase for later use; d. adding phase B as thickener into phase A, homogenizing for 3 min at 70-80deg.C, and confirming dispersed state; e. d, adding phase C into the content obtained in the step d, homogenizing and emulsifying for 5 minutes, controlling the temperature to be 70-80 ℃, and confirming the emulsifying state; f. and D, cooling the content obtained in the step e to 40-45 ℃, adding the phase D, and homogenizing for 3 minutes to obtain the eye essence emulsion.
TABLE 6
Experimental example 5: safety patch test
Taking an eye essence milk sample prepared in experimental example 4 in a plaque tester, wherein a control hole is a blank control; the patch tester added with the test object is stuck to the forearm flexor side of the tested person, and is uniformly stuck to the skin by the light palm pressure for 24 hours; skin irritation and sensitization were observed as in table 7 after 30min, 24 hr, 48 hr, respectively, after removal of the subject plaque tester, and the observations were recorded.
TABLE 7 skin response grading Standard for skin closed Patch test
Experimental results: the samples were subjected to a human skin patch test, and the results are shown in Table 8. The test result of the human body skin patch shows that 30 people have no skin adverse reaction.
TABLE 8 cosmetic human skin Patch test results
Experimental example 6: test of effect of removing black eyes
(1) Number of test persons: 5 people;
(2) Test site: an eye;
(3) The eye melanin content was measured with skin melanin and heme tester Mexameter MX18 before, 2 weeks after, and 4 weeks after the use of the product, respectively, by selecting 5 volunteers (except pregnant or lactating women) aged 18-60 and having dark circles, using eye essence milk samples, each time in the morning and evening. The efficacy of improving black eye is evaluated by increasing and decreasing the melanin content of eyes, and the effect is better as the melanin content is decreased more. (melanin content increasing rate = (melanin content after use-melanin content before use)/(melanin content before use ×100%)
Experimental results: the results are shown in Table 9, and the samples are effective in improving dark circles.
TABLE 9 increase and decrease of melanin content (%)
| Before use | For 2 weeks | 4 weeks of | |
| Sample of | 0 | -17.9 | -20.2 |
Experimental example 7: pouch removal effect test
The eye pouch volume was measured with an Antera 3D (Miravex, ireland) before and after 2 weeks of use of the product, respectively, by selecting 5 volunteers (except pregnant or lactating women) aged 18-60 years and having an eye pouch, using eye essence milk samples, once a day each in the morning and evening. The experimental results are shown in fig. 1, and the sample can effectively improve the problem of the eye bags.
There are many other embodiments that can be implemented, and none of them are listed here, but the embodiments claimed in the claims of this application are all applicable.
What is not described in detail in the present specification is common general knowledge of a person skilled in the art.
As used throughout the specification and claims, the word "comprise" is an open-ended term, and thus should be interpreted to mean "include, but not limited to. By "substantially" is meant that within an acceptable error range, a person skilled in the art is able to solve the technical problem within a certain error range, substantially achieving the technical effect.
It should also be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a product or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such product or system. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a commodity or system comprising such elements.
While the foregoing description illustrates and describes the preferred embodiments of the present application, it is to be understood that this application is not limited to the forms disclosed herein, but is not to be construed as an exclusive use of other embodiments, and is capable of many other combinations, modifications and environments, and adaptations within the scope of the inventive concept described herein, through the foregoing teachings or through the skill or knowledge of the relevant arts. And that modifications and variations which do not depart from the spirit and scope of the present invention are intended to be within the scope of the appended claims.
Claims (3)
1. A method for preparing an eye repair composition, which is characterized by comprising the following components: 0.005 part of acetyl tetrapeptide-5, 4 parts of whitening plant extract composition and 4 parts of lentinus edodes mycelium extract are uniformly mixed, wherein the parts are calculated by weight;
the whitening plant extract composition consists of paeonia lactiflora root extract and reed extract in a weight ratio of 1:1;
the acetyl tetrapeptide-5 is pre-dispersed uniformly with 50% glycerol;
the whitening plant extract composition is obtained through the following preparation steps:
(1) Sun-drying radix paeoniae alba and reed, crushing, weighing according to the required parts, and uniformly mixing;
(2) Adding 25-40 times of deionized water, extracting at 75-95deg.C for 2-4 hr for 2-3 times, and mixing extractive solutions;
(3) Cooling the extract obtained in the step (2) to 30 ℃ or below, filtering with 100-200 mesh gauze, filtering twice, collecting filtrate to obtain primary filtrate, and naturally standing the primary filtrate for 24 hours;
(4) Centrifuging the solution after standing in the step (3) for 0.5-1 hour at a rotating speed of 2000-10000rpm, filtering with a filter membrane with a pore diameter of 0.1-1.0 μm, and collecting filtrate to obtain secondary filtrate;
(5) Concentrating the secondary filtrate obtained in the step (4) under reduced pressure, adding polyol, and uniformly mixing;
(6) Filtering the solution obtained in the step (5) by using a filter membrane with the pore diameter of 0.01-0.1 mu m, and collecting filtrate to obtain a whitening plant extract composition;
the weight of the secondary filtrate in the step (5) and the concentrated solution is 1/4-1/2 of the original weight;
the weight of the polyol added in the step (5) is 1-10 times of that of the concentrated solution, and the polyol is one or more of glycerol, 1, 3-butanediol, 1, 2-pentanediol, 1, 3-propanediol and dipropylene glycol.
2. An eye-repair composition prepared by the method of claim 1, comprising the following components: 0.005 parts of acetyl tetrapeptide-5, 4 parts of whitening plant extract composition, 4 parts of lentinus edodes mycelium extract, the parts being by weight;
the whitening plant extract composition comprises paeonia lactiflora root extract and reed extract in a weight ratio of 1:1.
3. Use of the eye-repair composition according to claim 2 in the cosmetic field, wherein the cosmetic is one of an eye cream, an eye gel, an eye cream, an eye essence, an eye mask, an eye patch, an eye gel, an eye makeup-removing product.
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