CN115869233A - Whitening and anti-wrinkle essence and preparation method thereof - Google Patents
Whitening and anti-wrinkle essence and preparation method thereof Download PDFInfo
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Abstract
The invention discloses whitening and anti-wrinkle essence and a preparation method thereof, and relates to the technical field of whitening cosmetics. The whitening anti-wrinkle composition comprises the following raw materials in parts by weight: 0.001 to 0.1 portion of schizonepeta spike extract, 0.002 to 0.2 portion of lichen extract, 0.01 to 0.24 portion of verbena extract, 0.05 to 0.3 portion of myrtle leaf extract, 0.003 to 0.1 portion of Chinese ligusticum rhizome extract and 0.01 to 0.1 portion of sodium hyaluronate. According to the invention, the specific synergistic effect among the components is utilized, so that the wrinkle of the skin is greatly improved, and the wrinkle-resistant effect is obvious. The whitening and anti-wrinkle composition provided by the invention is beneficial to improving the skin smoothness of a user, and has an obvious whitening effect. The prepared essence has the obvious effect of inhibiting tyrosinase, is mild in quality, safe and non-irritant, and has an obvious anti-wrinkle effect and a whitening effect.
Description
Technical Field
The invention relates to the technical field of whitening cosmetics, and particularly relates to whitening and anti-wrinkle essence and a preparation method thereof.
Background
Currently, whitening is mostly performed by physical concealer treatment using cosmetics containing titanium dioxide. The titanium dioxide is added into a certain substrate and uniformly coated on the face, the high reflection of the titanium dioxide to light is utilized to improve the brightness of the skin, and meanwhile, the titanium dioxide also fills in wrinkles on the surface of the skin, so that the effect of covering the wrinkles of the skin is achieved. However, such physical masking does not solve the problems of dullness and increased wrinkles from the viewpoint of the skin itself, and does not effectively delay the aging process of the skin. In addition, a laser spot removing method is adopted by some people, the method is expensive, spots are blackened, inflammation or more spread occurs, and the mild spot-lightening and spot-removing effects cannot be achieved.
Patent application with patent publication number CN 110151617A discloses an extraction process of myrtle extracting solution and application thereof, and the obtained myrtle-containing anti-wrinkle essence has the characteristics of resisting wrinkles and preventing skin aging. However, the essence has no whitening effect.
In view of this, the invention is particularly proposed.
Disclosure of Invention
The invention aims to provide an essence capable of whitening skin and resisting wrinkles and a preparation method thereof, and provides the essence capable of whitening skin and resisting wrinkles.
The invention is realized by the following steps:
in a first aspect, the invention provides a whitening and anti-wrinkle composition, which comprises the following raw materials in parts by weight: 0.001 to 0.1 portion of schizonepeta spike extract, 0.002 to 0.2 portion of lichen extract, 0.01 to 0.24 portion of verbena extract, 0.05 to 0.3 portion of myrtle leaf extract, 0.003 to 0.1 portion of ligusticum Liaoning extract and 0.01 to 0.1 portion of sodium hyaluronate.
The composition comprises herba Schizonepetae extract, lichen extract, herba Verbenae extract, myrtle leaf extract, ligusticum jezoides extract and sodium hyaluronate, and has skin whitening and wrinkle removing effects. The specific synergistic effect among the components enables the wrinkles of the skin to be improved to a great extent, and the anti-wrinkle effect is obvious. The whitening and anti-wrinkle composition provided by the invention is beneficial to improving the skin smoothness of a user, and has an obvious whitening effect.
Wherein the herba Schizonepetae extract is obtained from water or alcoholic solution of dry herba Schizonepetae. Herba Schizonepetae, pungent in flavor, slightly warm, entering liver and lung channels, has effects of relieving exterior syndrome, dispelling pathogenic wind, promoting eruption, and eliminating sore, and can be used for treating common cold, headache, measles, rubella, and pyocutaneous disease; modern pharmacological research shows that schizonepeta spike has the functions of antipyresis, anti-inflammation, analgesia, itching relieving and antivirus.
In the present invention, the moss extract is derived from an aqueous or alcoholic solution extract of moss. Lichen is a complex formed by symbiosis of fungi and algae, and has a long history in China, and the main active ingredients of lichen are lichen polysaccharide, allopatric lichen polysaccharide, beta-lichen phenol and lichenin, and the lichen has various biological activities of moisturizing, improving skin elasticity, fading texture, improving skin darkness, resisting oxidation, resisting tumors, resisting viruses, resisting bacteria and the like.
In the present invention, the verbena extract is derived from an aqueous or alcoholic solution of verbena. Herba Verbenae, also known as Spiranthis Praecocis, herba Schizonepetae, and Agrimonia pilosa, is dried aerial part of herba Verbenae of Verbenaceae, and is grown for many years, and dried whole herb or whole herb with root is used as medicine, and has light smell and slightly bitter taste; the main functional components are triterpenes, steroids, flavonoid polysaccharide, verbena, and the like, and the medicine has the effects of clearing away heat and toxic materials, promoting blood circulation and stimulating the menstrual flow, inducing diuresis and reducing edema, and preventing malaria, and is used for treating cold and fever, jaundice, dysentery, dysmenorrhea, edema, malaria, traumatic injury, and the like. In skin care, the cream can regulate water-oil balance, so that pores are compacted, and then the skin is firmer; in addition, the verbena also has strong antioxidant and anti-aging effects, and researches show that the verbena has good B16 cell proliferation inhibition effect.
In the present invention, the myrtle leaf extract is derived from an aqueous or alcoholic solution of myrtle leaves. Myrtle, also known as cyclopentadiene and myrcia, is a evergreen shrub of myrtle of Myrtaceae, and has astringent, tranquilizing, hemostatic, and tonic effects; can be used for treating digestive tract, respiratory tract and various infectious diseases by oral administration, and can be used for treating various skin infections, ulcer, etc. by external application; the myrtle leaves contain more plant polyphenol substances, and the plant polyphenol substances are natural strong reducing substances and have the characteristics of being stronger than common antioxidants in the aspects of oxidation resistance and free radical removal.
In the invention, the Ligusticum jeholense extract is derived from the water or alcohol solution extract of Ligusticum jeholense. Ligusticum jezoides, also named family Ligusticum sinense, etc. is the dried rhizome and root of Ligusticum jezoides of Umbelliferae, and contains linoleic acid, ligustilide, and coumarin; has pungent taste and warm nature, has effects of dispelling pathogenic wind, dispelling cold, eliminating dampness, and relieving pain, and can be used for treating wind-cold type common cold, belladonna pain, and rheumatism arthralgia; modern pharmacological experiments prove that the ligusticum sinense has various pharmacological effects of resisting inflammation, easing pain, tranquilizing, relieving fever, relieving spasm, resisting ulcer, benefiting gallbladder, resisting bacteria and the like. It is commonly used in ancient medical books for whitening skin, lightening spots and brightening.
According to the invention, the sodium hyaluronate is composed of different molecular weights, and researches show that the sodium hyaluronate with different molecular weights has obvious physiological action difference, the sodium hyaluronate with large molecular weight has strong water locking capacity and has the effect of moisturizing the skin, and the sodium hyaluronate with small molecular weight can penetrate into the dermis of the skin to achieve deep water replenishing, even exert the effects of repairing cuticle forming cells, stimulating the regeneration of collagen and restoring and resisting aging. Hyaluronic acid with large and small molecular weights can synergistically enhance the moisturizing and anti-aging effects.
Sodium hyaluronate has several effects as follows: (1) moisture retention. The moisturizing effect is the most important effect of sodium hyaluronate in cosmetics, and compared with other moisturizers, the sodium hyaluronate has the highest moisture absorption capacity in a low-humidity environment and the lowest moisture absorption capacity in a high-humidity environment, and the unique property can meet the requirements of skin on the moisturizing effect of cosmetics in different seasons and different environmental humidities. (2) has nourishing effect. The sodium hyaluronate is a biological substance inherent in a human body, and is supplemented by exogenous sodium hyaluronate to human endogenous hyaluronic acid. (3) repairing and preventing effects. Sodium hyaluronate can promote proliferation and differentiation of epidermal cells, scavenge oxygen free radicals, and regenerate damaged skin. (4) lubricity and film-forming properties. After being coated on the skin, the sodium hyaluronate can form a layer of film on the surface of the skin, so that the skin can generate good smooth feeling and lubricating feeling, and the sodium hyaluronate plays a role in protecting the skin.
In the invention, the schizonepeta spike extract mainly has the functions of diminishing inflammation and relieving itching, and the schizonepeta spike extract and the hyaluronic acid are mixed and matched with at least one or two of other extracts (lichen extract, verbena extract, myrtle leaf extract and ligusticum Liaoning extract), so that the whitening, anti-aging, repairing and synergistic effects can be achieved.
In an alternative embodiment, the spica schizonepetae extract, the lichen extract, the verbena extract, the myrtle leaf extract and the ligusticum Liaoning extract are all in a transparent liquid state.
The above extracts include, but are not limited to, extracts obtained by water extraction, alcohol extraction, etc. Including but not limited to crude extracts, fine extracts.
In a preferred embodiment of the invention, the sodium hyaluronate comprises two sodium hyaluronate of different molecular weights: macromolecular sodium hyaluronate with the molecular weight of 200-500 ten thousand daltons and micromolecular sodium hyaluronate with the molecular weight of 500-1000 daltons, wherein the mass ratio of the macromolecular sodium hyaluronate to the micromolecular sodium hyaluronate is 1:1-5.
The large molecular hyaluronic acid and the small molecular hyaluronic acid are compounded, so that the large molecular hyaluronic acid can retain water and supplement water, and the small molecular hyaluronic acid can quickly permeate into a dermis layer to play the effects of repairing and resisting aging. The combination of the two greatly improves the skin barrier and repairs and resists aging.
The mass ratio of the macromolecular sodium hyaluronate to the micromolecular sodium hyaluronate is 1:1, or 1:2, or 1:3, or 1:4 or 1:5. The inventors found that continuing to increase the mass ratio of small molecules (beyond 1:5), the efficiency was not significant, and that small molecule hyaluronic acid was not cost effective with increasing ratio due to its higher price.
In a second aspect, the invention also provides a whitening and anti-wrinkle essence which comprises the following raw materials in parts by weight: 5.2 to 25 portions of water phase, 1 to 5 portions of the whitening and anti-wrinkle composition, 1 to 5 portions of skin moistening phase, 0.1 to 1 portion of preservative and 0.001 to 0.1 portion of aromatic (selected from natural essential oil and/or synthetic essence).
The essence provided by the invention has the obvious effect of inhibiting tyrosinase, is mild in quality, safe and non-irritant, and has an obvious anti-wrinkle effect and a whitening effect.
The whitening and anti-wrinkle essence comprises the following raw materials in parts by weight: 6 to 20 portions of water phase, 1 to 5 portions of the whitening and anti-wrinkle composition, 1 to 5 portions of skin moistening phase, 0.1 to 1 portion of preservative and 0.01 to 0.1 portion of aromatic.
In a preferred embodiment of the application of the present invention, the aqueous phase comprises the following raw materials in parts by weight: 1 to 2 parts of diglycerin, 1 to 10 parts of glycerol, 0.1 to 1 part of xanthan gum, 1 to 5 parts of emulsifier, 2 to 5 parts of fucooligosaccharide and 0.1 to 2 parts of thickener.
In a preferred embodiment of the present invention, the emulsifier is selected from at least one of lecithin, inulin lauryl carbamate, cetearyl olive oleate, sorbitan olive oleate, cetearyl glucoside, C12-20 alkyl glucoside and methyl glucose sesquistearate.
In a preferred embodiment of the invention, the thickener is at least one selected from the group consisting of ammonium acryloyldimethyltaurate/VP copolymer, carbomer and polyacrylamide.
In an alternative embodiment, the thickener is selected from the group consisting of ammonium acryloyl dimethyl taurate/VP copolymer.
In a preferred embodiment of the present invention, the emollient phase is selected from the group consisting of: one or more of shea butter, avocado oil, coconut oil, jojoba oil, macadamia nut oil, egg oil, arnebia oil, squalane, olive oil, silicone, and cetyl ethylhexanoate;
the siloxane is selected from water soluble polydimethylsiloxane.
In a preferred embodiment of the application of the invention, the preservative is selected from at least one of phenoxyethanol, ethylhexyl glycerol, potassium sorbate, sodium benzoate and benzyl alcohol;
in an alternative embodiment, the preservative is selected from phenoxyethanol and ethylhexylglycerin.
The whitening anti-wrinkle essence comprises the following raw materials in parts by weight: 1-2 parts of diglycerol, 1-10 parts of glycerol, 0.1-1 part of xanthan gum, 1-5 parts of lecithin, 2-5 parts of fucooligosaccharide and 0.1-2 parts of acryloyl dimethyl taurate/VP copolymer;
whitening and anti-wrinkle composition: 0.001 to 0.1 portion of schizonepeta spike extract, 0.002 to 0.2 portion of lichen extract, 0.01 to 0.24 portion of verbena extract, 0.05 to 0.3 portion of myrtle leaf extract, 0.003 to 0.1 portion of Chinese ligusticum rhizome extract and 0.01 to 0.1 portion of sodium hyaluronate;
and (3) a skin moistening phase: 1-5 parts of water-soluble polydimethylsiloxane;
0.1 to 1 portion of mixture of preservative phenoxyethanol and ethylhexyl glycerin and 0.001 to 0.1 portion of aromatic.
In a third aspect, the invention also provides a preparation method of the essence, which comprises the following steps:
adding the water phase into water, stirring and homogenizing, regulating the temperature of the mixed solution to 80-85 ℃, and preserving heat; then cooling to 50-55 ℃, adding the whitening and anti-wrinkle composition dispersed by glycerol, and continuously stirring uniformly; cooling to 40-45 ℃, adding the skin moistening phase, stirring, cooling to 38-40 ℃, adding the preservative and the aromatic, and stirring to obtain the essence.
Under the conditions, the whitening and anti-wrinkle essence can be quickly and simply prepared. The raw materials for preparing the essence are added and mixed in batches, and the preparation temperature is controlled, so that the obtained essence has the advantages of clear effect, safety, no adverse reaction, wrinkle resistance, repair and whitening.
In the preferred embodiment of the invention, the water phase is added into water with the temperature of 80-85 ℃, stirred and homogenized, and then the temperature is kept for 20-30 minutes.
The invention has the following beneficial effects:
the efficacy composition provided by the invention consists of schizonepeta spike extract, lichen extract, verbena extract, myrtle leaf extract, ligusticum Liaoning extract and sodium hyaluronate, and has whitening and anti-wrinkle effects. The specific synergistic effect among the components enables the wrinkles of the skin to be greatly improved, and the anti-wrinkle effect is obvious. The whitening and anti-wrinkle composition provided by the invention is beneficial to improving the skin smoothness of a user, and has an obvious whitening effect.
The prepared essence has the remarkable effect of inhibiting tyrosinase, is mild, safe and non-irritant in quality, and has the obvious effects of improving the anti-wrinkle effect and the whitening effect.
In addition, the preparation method of the essence is provided, and the essence with whitening and anti-wrinkle effects can be quickly and simply prepared by the preparation method. The raw materials for preparing the essence are added and mixed in batches, and the preparation temperature is controlled, so that the obtained essence has the advantages of clear effect, safety, no adverse reaction, wrinkle resistance, repair and whitening.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
The whitening and anti-wrinkle essence comprises the following components:
water phase: 1.5 parts of diglycerol, 7 parts of glycerol, 0.1 part of xanthan gum, 2 parts of lecithin, 2 parts of fucooligosaccharide and 0.9 part of acryloyl dimethyl taurate/VP copolymer;
whitening anti-wrinkle composition (efficacy composition): 0.01 part of schizonepeta spike extract, 0.06 part of lichen extract, 0.1 part of verbena extract, 0.16 part of myrtle leaf extract, 0.05 part of ligusticum Liaoning extract and 0.06 part of sodium hyaluronate (the mass ratio of 200-500 kilodalton macromolecular sodium hyaluronate to 500-1000 dalton micromolecular sodium hyaluronate is 1:2);
and (3) a skin moistening phase: 2.5 parts of water-soluble polydimethylsiloxane;
preservative: 0.8 part of a mixture of phenoxyethanol and ethylhexyl glycerol;
0.03 part of aromatic.
The preparation method comprises the following steps:
(1) Adding deionized water into a stirring pot, heating to 83 ℃, sequentially adding the raw materials of the water phase, stirring, homogenizing and then preserving heat for 20 minutes;
(2) Cooling to 55 deg.C, adding the effective composition dispersed with glycerol, and stirring;
(3) And cooling to 45 ℃, adding a skin moistening phase, stirring and cooling to 38 ℃, adding a preservative and an aromatic, and stirring for 30 minutes to obtain the whitening and anti-wrinkle essence liquid.
Example 2
The whitening and anti-wrinkle essence comprises the following components:
water phase: 2 parts of diglycerin, 3 parts of glycerol, 0.15 part of xanthan gum, 1.2 parts of lecithin, 2 parts of fucooligosaccharide and 0.5 part of acryloyl dimethyl ammonium taurate/VP copolymer;
whitening anti-wrinkle composition (efficacy composition): 0.018 parts of schizonepeta spike extract, 0.042 parts of lichen extract, 0.08 parts of verbena extract, 0.2 parts of myrtle leaf extract, 0.062 parts of ligusticum Liaoning extract and 0.03 parts of sodium hyaluronate (the mass ratio of 200-500 ten thousand daltons of macromolecular sodium hyaluronate to 500-1000 daltons of small molecular sodium hyaluronate is 1:1);
and (3) a skin moistening phase: 4.5 parts of water-soluble polydimethylsiloxane;
preservative: 0.6 part of a mixture of phenoxyethanol and ethylhexyl glycerol;
0.02 part of aromatic.
The preparation method comprises the following steps:
(1) Adding deionized water into a stirring pot, heating to 85 ℃, sequentially adding the raw materials of the water phase, stirring, homogenizing and then preserving heat for 20 minutes;
(2) Cooling to 50 deg.C, adding the effective composition dispersed with glycerol, and stirring;
(3) And cooling to 42 ℃, adding a skin moistening phase, stirring and cooling to 38 ℃, adding a preservative and an aromatic, and stirring for 30 minutes to obtain the whitening and anti-wrinkle essence.
Example 3
The whitening anti-wrinkle essence comprises the following components:
water phase: 1.6 parts of diglycerol, 3.8 parts of glycerol, 0.64 part of xanthan gum, 4 parts of lecithin, 5 parts of fucooligosaccharide and 1.6 parts of acryloyl dimethyl ammonium taurate/VP copolymer;
whitening anti-wrinkle composition (efficacy composition): 0.038 part of schizonepeta spike extract, 0.12 part of lichen extract, 0.16 part of verbena extract, 0.2 part of myrtle leaf extract, 0.048 part of ligusticum Liaoning extract and 0.05 part of sodium hyaluronate (the mass ratio of 200-500 million daltons of macromolecular sodium hyaluronate to 500-1000 daltons of small molecular sodium hyaluronate is 1:4);
and (3) a skin moistening phase: 4.0 parts of water-soluble polydimethylsiloxane;
preservative: 0.5 part of a mixture of phenoxyethanol and ethylhexyl glycerol;
0.08 part of aromatic.
The preparation method comprises the following steps:
(1) Adding deionized water into a stirring pot, heating to 80 ℃, sequentially adding the raw materials of the water phase, stirring, homogenizing and then preserving heat for 30 minutes;
(2) Cooling to 52 deg.C, adding the effective composition dispersed with glycerol, and stirring;
(3) And cooling to 42 ℃, adding a skin moistening phase, stirring and cooling to 39 ℃, adding a preservative and an aromatic, and stirring for 30 minutes to obtain the whitening and anti-wrinkle essence.
Comparative example 1
Compared with example 1, the only difference is that: the efficacy composition was replaced with the same amount of schizonepeta spike extract, and the composition and preparation method of the remaining essence were the same as in example 1.
Comparative example 2
Compared with example 1, the difference is only that: the efficacious composition was replaced with equal amounts of lichen extract and the composition and preparation of the remaining essences were the same as in example 1.
Comparative example 3
Compared with example 1, the difference is only that: the composition and preparation method of the other essences were the same as in example 1, except that the effective composition was replaced with the equivalent amount of verbena extract.
Comparative example 4
Compared with example 1, the only difference is that: the efficacy composition was replaced with an equal amount of myrtle leaf extract, and the composition and preparation method of the remaining essence were the same as in example 1.
Comparative example 5
Compared with example 1, the difference is only that: the effective composition was replaced with the equivalent amount of ligusticum Liaoning extract, and the composition and preparation method of the other essence were the same as in example 1.
Comparative example 6
Compared with example 1, the difference is only that: the efficacy composition was replaced with an equal amount of sodium hyaluronate (large molecule: small molecule = 1:2) and the composition and preparation of the remaining serum was the same as in example 1.
Comparative example 7
Compared with example 1, the only difference is that: only the myrtle leaf extract in the efficacy composition was replaced with the same amount of the alpine lupulus extract, and the composition of the remaining efficacy composition, the composition of the essence and the preparation method were the same as in example 1.
Comparative example 8
Compared with example 1, the difference is only that: only the verbena extract in the efficacy composition was replaced with the same amount of magnolia alba extract, and the composition, the composition of the essence and the preparation method of the remaining efficacy composition were the same as in example 1.
Comparative example 9
Compared with example 1, the difference is only that: only the lichen extract in the efficacy composition was replaced with the same amount of evening primrose extract, and the composition, the composition of the essence and the preparation method of the remaining efficacy composition were the same as in example 1.
Experimental example 1
Tyrosinase activity inhibition experiments were performed.
The principle is as follows: tyrosinase is a key enzyme in skin melanin biosynthesis, and acts on dopa to form dopaquinone, which spontaneously undergoes a series of reactions to finally form melanin. Tyrosinase can catalyze the conversion of dopa to dopaquinone in a phosphoric acid solution with pH 6.8, and the absorbance can be measured at 475nm of a spectrophotometer. The raw material with tyrosinase activity inhibition effect can reduce conversion of dopa into dopaquinone, so as to reduce light absorption value, and the inhibition effect of the cosmetic on tyrosinase activity is evaluated according to the change of the light absorption value. Further, the inhibitory effect of the cosmetic on skin melanin production was evaluated.
Preparation of test sample solution: 1mL of each of the essences of example 1, the blank control group and comparative examples 1 to 9 (see Table 1) was taken, diluted 1mL with a disodium hydrogenphosphate-citric acid buffer solution (pH 6.8), and mixed to prepare sample solutions.
TABLE 1 test groups
| Group of | Formulation(s) |
| Blank control group | The functional composition was replaced with an equal amount of glycerin, otherwise as in example 1 |
| Comparative example 1 | The effective composition was replaced with equal amount of herba Schizonepetae extract, as in example 1 |
| Comparative example 2 | Replacing the effective composition with equal amount of lichen extractThe same procedure as in example 1 was repeated |
| Comparative example 3 | The functional composition was replaced by an equal amount of verbena extract, otherwise the same as example 1 |
| Comparative example 4 | The efficacy composition was changed to equal amount of myrtle leaf extract, otherwise as in example 1 |
| Comparative example 5 | The effective composition is replaced by equal amount of Ligusticum jeholense extract, other examples are the same as example 1 |
| Comparative example 6 | The efficacy composition was changed to an equal amount of sodium hyaluronate (large molecule: small molecule = 1:2) and the other examples were the same as example 1 |
| Comparative example 7 | The extract of the leaf of myrtle of phase B was replaced by the same amount of the extract of the leaf of the plant of the same type as that of the leaf of the myrtle of phase B, and the same procedure as that of example 1 was repeated |
| Comparative example 8 | The phase B ingredient herba Verbenae extract is replaced by equal amount of Magnolia alba extract, otherwise the same as example 1 |
| Comparative example 9 | The moss extract of phase B is replaced by the same amount of evening primrose extract, otherwise the same as example 1 |
Use of 10mL test tubes to set up sample tubes (T), sample background (T) 0 ) Enzyme reaction tube (C) and solvent background (C) 0 ) Sample tube (T) and sample background (T) 0 ) In each case add1mL of sample solution, enzyme reaction tube (C) and solvent background (C) 0 ) Then 1mL of disodium hydrogen phosphate-citric acid buffer (see Table 2) was added.
0.5mL of tyrosinase solution (100 u/mL) and sample background (T) were added to each of the sample tube (T) and the enzyme reaction tube (C) 0 ) With background of solvent (C) 0 ) Replace with 0.5mL disodium hydrogen phosphate-citric acid buffer, mix the sample and tyrosinase well, put in 37 ℃ water bath and incubate for 10 minutes. 2mL of levodopa solution (1 mg/mL) was added to each tube in sequence, the reaction time was controlled to 5 minutes per tube, and each tube was immediately transferred to a cuvette and the absorbance was measured at 475 nm.
TABLE 2 sample charging meter
Tyrosinase inhibition rate calculation formula:
in the formula:
t-sample tube light absorption value, namely the light absorption value of the solution after the sample reacts with tyrosinase;
T 0 -sample background absorbance;
c, averaging the light absorption values of the enzyme reaction tube for 3 times, namely the light absorption value of the reaction of tyrosinase and dopa when no sample is added;
C 0 -background absorbance of solvent.
The results of the tyrosinase inhibition experiments are shown in table 3.
TABLE 3 tyrosinase inhibition experiment results
| Serial number | Group of | Inhibition ratio (%) |
| 1 | Example 1 | 72.2±3.54 |
| 2 | Blank control group | 10.5±4.16 |
| 3 | Comparative example 1 | 22.3±5.21 |
| 4 | Comparative example 2 | 35.6±4.83 |
| 5 | Comparative example 3 | 32.7±4.97 |
| 6 | Comparative example 4 | 28.8±3.94 |
| 7 | Comparative example 5 | 29.5±4.52 |
| 8 | Comparative example 6 | 40.1±5.12 |
| 9 | Comparative example 7 | 62.5±3.86 |
| 10 | Comparative example 8 | 58.6±4.37 |
| 11 | Comparative example 9 | 55.9±5.18 |
The results in table 3 show that the essence containing a single component in the same amount has a certain tyrosinase inhibition ability, but the inhibition effect on tyrosinase is not significant, and the inhibition effect on tyrosinase of the essence containing the composition is improved by more than 1 time, which indicates that the composition achieves a synergistic effect. In addition, the myrtle leaf extract, the verbena extract and the lichen extract in the efficacy composition are respectively replaced by the Tianshan eclipta extract, the magnolia alba extract and the evening primrose extract in equal amount, the inhibition effect on tyrosinase is not good as that of the example 1, and the synergy of the efficacy composition with the specific composition provided by the invention is better than that of other extracts.
Experimental example 2
And (5) safety test.
The safety test method comprises the following steps: 90 volunteers were selected, and the whitening and repairing essence liquids of examples 1 to 3 were tested according to the regulation of "human skin patch test" of technical safety standards for cosmetics (2015 edition) (30 in each example), and the skin reaction after 24 hours was observed by naked eyes, and the degree of reaction and the grade were shown in table 4.
TABLE 4 Patch test skin response rating Scale
The test results show that 90 volunteers also showed negative reactions. According to technical standards for cosmetic safety (2015), the evaluation criteria of the patch test of human body, and example 1 has no adverse reaction to human body. The whitening anti-wrinkle essence provided by the invention is mild in quality, safe and non-irritant.
Experimental example 3
And (5) an anti-wrinkle effect test.
Selecting 110 female volunteers with healthy skin, randomly dividing the facial wrinkles or fine lines into 11 groups of 10 persons each; the essences of example 1, blank control and comparative examples 1 to 9 were used, respectively. After 8 weeks of continuous use, the reduction degree of the wrinkle area, the number of wrinkles and the wrinkle depth of the face was evaluated by comparing the results at 0 week with the VISIA skin test apparatus, and the test results are shown in table 5.
Table 5 results of the effect test
| Serial number | Group of | Reduction of wrinkle area/%) | Reduction of number of wrinkles/%) | Reduction of wrinkle depth/%) |
| 1 | Example 1 | 28.0 | 26.5 | 23.1 |
| 2 | Blank control group | 5.2 | 3.8 | 2.5 |
| 3 | Comparative example 1 | 8.3 | 11.0 | 10.6 |
| 4 | Comparative example 2 | 11.6 | 12.3 | 9.1 |
| 5 | Comparative example 3 | 10.5 | 12.4 | 11.9 |
| 6 | Comparative example 4 | 14.3 | 12.5 | 11.6 |
| 7 | Comparative example 5 | 12.8 | 12.3 | 10.7 |
| 8 | Comparative example 6 | 14.8 | 12.9 | 13.4 |
| 9 | Comparative example 7 | 23.2 | 20.6 | 19.5 |
| 10 | Comparative example 8 | 25.8 | 21.3 | 19.8 |
| 11 | Comparative example 9 | 23.9 | 20.8 | 18.3 |
The results in table 5 show that the essence containing a single component in the same amount has a certain anti-wrinkle effect, and can reduce the area or degree of facial wrinkles of a volunteer, but the anti-wrinkle effect is not significant, and the anti-wrinkle effect of the essence containing the composition is significantly improved, which further indicates that the composition can achieve a synergistic effect in anti-wrinkle. The anti-wrinkle effect of the functional composition provided by the invention is inferior to that of the example 1 because the myrtle leaf extract, the verbena extract and the lichen extract in the functional composition are replaced by the Tianshan eclipta extract, the magnolia alba extract and the evening primrose extract correspondingly in equal amount, which shows that the synergistic effect of the functional composition provided by the invention is better than that of other extracts, and the anti-wrinkle effect is reduced because of the replacement of other extracts.
Experimental example 4
And (5) testing the whitening effect.
1) Test method
The volunteers are 40-50 years old, half of both males and females have obvious wrinkles or fine lines on the faces, voluntarily participate in the test, sign an informed consent before the test, cannot use any cosmetics 2 days before the test, and cannot use any cosmetics except for the test sample in the test process. After washing the face with warm water, the whitening and repairing essence of example 1 was applied to one face once in the morning and at night, and the test was conducted for 5 weeks. On the other half of the face, the serum of the blank control group (serum prepared by replacing the efficacy composition with an equivalent amount of glycerin only) was used.
2) Evaluation criteria
The test subjects evaluated their own skin changes.
3) Evaluation results
Through statistics, 90% of the testers evaluate that the facial skin of the testers is greatly improved, and particularly, the facial gloss of the testers is improved, the skin wrinkles are improved, the skin becomes smooth, and the whitening effect is obvious after the whitening and repairing essence is used.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The whitening and anti-wrinkle composition is characterized by comprising the following raw materials in parts by weight: 0.001 to 0.1 portion of schizonepeta spike extract, 0.002 to 0.2 portion of lichen extract, 0.01 to 0.24 portion of verbena extract, 0.05 to 0.3 portion of myrtle leaf extract, 0.003 to 0.1 portion of Chinese ligusticum rhizome extract and 0.01 to 0.1 portion of sodium hyaluronate.
2. The whitening and anti-wrinkle composition according to claim 1, wherein the sodium hyaluronate comprises two sodium hyaluronate of different molecular weights: macromolecular sodium hyaluronate with the molecular weight of 200-500 ten thousand daltons and micromolecular sodium hyaluronate with the molecular weight of 500-1000 daltons, wherein the mass ratio of the macromolecular sodium hyaluronate to the micromolecular sodium hyaluronate is 1:1-5.
3. The whitening anti-wrinkle essence is characterized by comprising the following raw materials in parts by weight: 5.2 to 25 parts of water phase, 1 to 5 parts of whitening and anti-wrinkle composition according to claim 1 or 2, 1 to 5 parts of skin moistening phase, 0.1 to 1 part of preservative and 0.001 to 0.1 part of aromatic.
4. The essence of claim 3, wherein the water phase comprises the following raw materials in parts by weight: 1 to 2 parts of diglycerin, 1 to 10 parts of glycerol, 0.1 to 1 part of xanthan gum, 1 to 5 parts of emulsifier, 2 to 5 parts of fucooligosaccharide and 0.1 to 2 parts of thickener.
5. The essence of claim 4, wherein the emulsifier is selected from at least one of lecithin, inulin lauryl carbamate, cetearyl olive oleate, sorbitan olive oleate, cetearyl glucoside, C12-20 alkyl glucoside, and methyl glucose sesquistearate.
6. The serum according to claim 4, characterized in that the thickener is selected from at least one of ammonium acryloyldimethyltaurate/VP copolymer, carbomer and polyacrylamide;
preferably, the thickener is selected from the group consisting of ammonium acryloyldimethyltaurate/VP copolymers.
7. The essence of claim 3, wherein the emollient phase is selected from the group consisting of: one or more of shea butter, avocado oil, coconut oil, jojoba oil, macadamia nut oil, egg oil, arnebia oil, squalane, olive oil, silicone, and cetyl ethylhexanoate;
the siloxane is selected from water-soluble polydimethylsiloxane.
8. The essence of claim 3, wherein the preservative is selected from at least one of phenoxyethanol, ethylhexyl glycerin, potassium sorbate, sodium benzoate, and benzyl alcohol;
preferably, the preservative is selected from phenoxyethanol and ethylhexyl glycerol.
9. The method for preparing the essence according to any one of claims 3 to 8, which comprises the following steps:
adding the water phase into water, stirring and homogenizing, regulating the temperature of the mixed solution to 80-85 ℃, and preserving heat; then cooling to 50-55 ℃, adding the whitening and anti-wrinkle composition dispersed by glycerol, and continuously stirring uniformly; cooling to 40-45 ℃, adding the skin moistening phase, stirring, cooling to 38-40 ℃, adding the preservative and the aromatic, and stirring to obtain the essence.
10. The preparation method of claim 9, wherein the aqueous phase is added to water at 80-85 ℃, stirred, homogenized and then kept for 20-30 minutes.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116270365A (en) * | 2023-04-28 | 2023-06-23 | 康博士日化集团有限公司 | Freckle-removing plant extract composition and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116270365A (en) * | 2023-04-28 | 2023-06-23 | 康博士日化集团有限公司 | Freckle-removing plant extract composition and preparation method and application thereof |
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