KR102142426B1 - Cosmetic materials composition for skin-whitening and skin-soothing, Cosmetic materials using the same and Manufacturing method thereof - Google Patents

Abstract

The present invention relates to a cosmetic composition and a cosmetic material prepared using the same. More particularly, the present invention relates to a cosmetic composition capable of ameliorating atopic dermatitis while having excellent skin whitening and skin soothing effects, a cosmetic material (and/or cosmetics) using the same, and a method of preparing the same. The cosmetic composition comprises a plant-extracted complex fermentation product, a complex oil, a moisturizing agent, a skin function improving agent, a pH adjusting agent, a surfactant, arginine, a color developing agent and floral water.

Description

Cosmetic materials composition for skin-whitening and skin-soothing, Cosmetic materials using the same and Manufacturing method thereof

The present invention relates to a cosmetic that has excellent skin whitening and soothing effects derived from natural plant components using natural plant extract components and the like, and has an effect of improving atopic dermatitis, and a method of manufacturing the same.

The skin is a single membrane covering the outside of the body and plays a role in protecting and regulating internal organs and other internal organs by performing various physiological functions that protect the body from environmental factors such as various external stimuli, obstacles, and dryness. do.

However, with age, secretion of various hormones that regulate metabolism internally decreases, and functions of immune cells and activity of cells decrease. In addition, externally, the content of ultraviolet rays in the sun rays increases due to the destruction of the ozone layer, and excessive physical and chemical irritation and stress caused by the increase of free radicals and active harmful oxygen, etc., as the environmental pollution becomes more serious, is the normal function of the skin. It damages the skin by promoting deterioration, formation of spots, freckles, etc. due to melanin deposition and skin aging. In order to prevent skin aging and damage caused by such internal and external factors, and to maintain healthier and more beautiful skin, physiologically active substances obtained from various animals, plants, microorganisms, etc. are added to cosmetics to maintain the skin's own function and Efforts to maintain skin health by activating cells to promote skin metabolism are continuing, and cosmetics and external preparations for skin are being developed accordingly.

Atopy occurs mainly in infancy and is also called prenatal fever, and mainly occurs as the content of ceramides in the stratum corneum decreases. Although the cause of atopic dermatitis is not yet clearly identified, it is presumed to be caused by disturbance of the immune system.

In general, atopic dermatitis is dry as the moisture retention function of the stratum corneum decreases, and skin allergies easily occur as the skin barrier function decreases, resulting in severe itching, recurring recurrence, and easily causing dermatitis due to various stimuli. do. In many cases, it is repeatedly exacerbated at intervals of 1 to 2 weeks and, if not properly managed, progresses to a subacute lesion of erythematous rash accompanied by effusion, and a chronic lesion of lichen planus thickened like leather.

There are two main causes of atopic skin. One is due to a genetic cause. The skin protective film due to innate lack of gamma-linoleic acid (GLA), an intercellular binding material, is a skin protective film. This is weak and occurs when moisture on the skin surface is easily lost. Other causes are environmental causes, which are caused by dry living environments, free radicals, bacteria, viruses, fungi, etc. [Dermatology, Korean Dermatological Society Publication Committee, Moongak Yeo, 1992].

Due to atopic dermatitis, mast cells exhibit degranulation by releasing allergic reaction-inducing substances such as histamine from intracellular granules [Gao ZG, et al., Biochem Pharmacol. 2009. Nov 5]. In addition, atopic dermatitis has been reported to be highly likely to be an allergic disease in that it accompanies allergic rhinitis and asthma [de Meer G, et al., Pediatr Allergy Immunol. 2009. Nov 13].

Among the most commonly used treatment drugs, steroid drugs (adrenal corticosteroids) have excellent anti-inflammatory and immune suppression effects, but if used for a long time, the skin may become hairy and skin atrophy, skin pigmentation decreases, and bacterial infection. It has side effects such as acne, thinning of the skin and revealing thread veins.

A lot of research and products are being developed for various atopic dermatitis improvement and/or prevention ingredients derived from natural materials using chemicals, and a cosmetic that can be applied to various formulations and applications while minimizing side effects such as skin troubles. There is an increasing demand for

Korean Patent Registration Publication No. 10-0847104 (announced on 2008.07.11) Korean Patent Application Publication No. 10-2012-015881 (published on February 22, 2012)

As a result of trying to develop a cosmetic product that has an effect of improving atopic dermatitis, improving skin whitening, and soothing the skin while preventing and minimizing skin side effects, the present invention has learned a cosmetic composition having an optimal composition and composition ratio, and a cosmetic product using the same. The invention was completed. That is, the present invention is an object of the present invention to provide a cosmetic and a manufacturing method using the composition.

In order to solve the above problems, the cosmetic composition of the present invention includes a plant-extracted complex fermented product, a complex oil, a moisturizing agent, a skin function improving agent, a pH adjusting agent, a surfactant, arginine, a color developing agent, and a residual amount of floral water. .

In addition, it is an object of the present invention to provide a cosmetic having a white-based color using the composition.

로 가온한 후, 초고압 분산기로 교반 및 혼합하는 공정을 포함한다.In addition, another object of the present invention is a method of manufacturing the cosmetic (or cosmetic), a plant-extracted complex fermented product, a complex oil, a moisturizing agent, a skin function improving agent, a pH adjusting agent, a surfactant, arginine, a color developing agent and a residual amount of floral Water 50 to 80

After heating to, a step of stirring and mixing with an ultra-high pressure disperser is included.

The cosmetic made with the composition of the present invention has an effect of improving atopic dermatitis, and can be used on various skin such as skin with skin troubles such as allergies, and has excellent skin soothing effect and excellent skin whitening improvement effect. Can provide.

1 is a graph for measuring a ^* value change and a ^* value improvement rate (%) for Example 1 conducted in Experimental Example 1.
2A to 2F are photographs of measuring skin soothing effects for Example 1 performed in Experimental Example 2.

Hereinafter, it will be described in more detail through the method of manufacturing a cosmetic (and/or cosmetic) excellent in skin whitening improvement and soothing effect of the present invention.

The cosmetic composition of the present invention is prepared by heating a cosmetic composition including a plant-extracted complex fermentation product, a complex oil, a moisturizer, a skin function improving agent, a pH adjuster, a surfactant, and a floral water, followed by stirring and mixing with an ultra-high pressure disperser. After heating a composition comprising a plant-extracted complex fermentation product, a complex oil, a moisturizing agent, a skin function improving agent, a pH adjusting agent, a surfactant, arginine, a color developing agent, and the remaining amount of floral water, It can be prepared by performing a process of stirring and mixing with an ultra-high pressure disperser.

The heating is 50 to 80°C, preferably 60 to 80°C, more preferably 70 to 80°C. At this time, if the temperature is less than 50°C, mixing between the compositions is not well made, so that the cosmetic ingredients are phase separated. There may be a problem that becomes, and if it exceeds 80°C, there may be a problem with the preservative power and/or the stability of the formulation, so it is good to perform stirring and mixing with an ultra-high pressure disperser at this temperature after heating to the above temperature range.

In addition, stirring and mixing with the ultra-high pressure disperser is performed at a pressure of 800 to 1,500 bar for 20 to 40 minutes, and preferably, mixing is performed at a pressure of 800 to 1,200 bar for 20 to 40 minutes. At this time, the internal temperature of the ultra-high pressure disperser is 50 to 80°C, and the outlet temperature of the ultra-high pressure disperser is about 10 to 15°C.

The plant-extracted complex fermented product is prepared by fermenting a plant-extracted mixture obtained by mixing 9 kinds of plant extracts with a fermentation filtrate, and preferably, in a mixture containing 0.5 to 2.0% by weight of licorice and the remaining amount of the plant-extracted mixture. The fermentation filtrate may be inoculated and fermented at a weight ratio of 1:0.10 to 0.20.

The plant extract mixture includes centella asiatica extract, blueberry extract, licorice extract, neem leaf extract, comfrey leaf extract, green tea extract, purslane extract, rosemary extract, and sugar elm bark extract. Preferably, based on 100 parts by weight of Centella asiatica extract, 60 to 100 parts by weight of Cheongho extract, 60 to 100 parts by weight of licorice extract, 30 to 70 parts by weight of neem leaf extract, 30 to 70 parts by weight of comfrey leaf extract, 15 to 50 parts by weight of green tea extract Parts, including 15 to 50 parts by weight of purslane extract, 10 to 45 parts by weight of rosemary extract and 10 to 45 parts by weight of sugar elm bark extract, more preferably 70 to 90 parts by weight of Cheongho extract based on 100 parts by weight of centella extract , Licorice extract 60 to 85 parts by weight, Neem leaf extract 35 to 55 parts by weight, Comfrey leaf extract 35 to 60 parts by weight, Green tea extract 25 to 40 parts by weight, Purslane extract 20 to 40 parts by weight, Rosemary extract 15 to 38 parts by weight and It is good to include 15 to 35 parts by weight of the dandelion bark extract.

At this time, if the amount of the cheongho extract is less than 60 parts by weight, there may be no skin regeneration effect on damaged skin, and if it exceeds 100 parts by weight, there may be a problem that increases the price and causes skin troubles. In addition, if the licorice extract is less than 60 parts by weight, there may be no soothing effect on angry skin, and if it exceeds 100 parts by weight, there may be a problem that only manufacturing cost increases due to excessive use. In addition, when the amount of the neem leaf extract is less than 30 parts by weight, there may be no effect of reducing itchiness and inflammation of the skin, and when it exceeds 70 parts by weight, there may be a problem that causes skin troubles. In addition, if the Comfrey leaf extract is less than 30 parts by weight, there may be no soothing effect on the private skin area, and if it exceeds 70 parts by weight, it is uneconomical. In addition, if the green tea extract is less than 15 parts by weight, there may not be an effect of reducing skin troubles through the antioxidant effect, and if it exceeds 50 parts by weight, it is uneconomical. In addition, if the amount of the purslane extract is less than 15 parts by weight, the effect of increasing skin trouble relief may decrease, and if it exceeds 50 parts by weight, there may be a problem in that fermentation by microorganisms is not well performed during fermentation. In addition, if the amount of the rosemary extract is less than 10 parts by weight, the effect of imparting vitality to the sagging skin tissue may be insufficient, and if it exceeds 45 parts by weight, it is uneconomical. In addition, when the amount of the sugar elm bark extract is less than 10 parts by weight, the effect of improving skin elasticity and skin vitality may be insufficient, and when it exceeds 45 parts by weight, there may be a problem in that fermentation by microorganisms does not work well during fermentation.

Each extract of the plant extract mixture may be prepared through a general extraction method used in the art, and preferably may be prepared through a vacuum low temperature extraction method, an ultrasonic extraction method, or the like. For example, the centella asiatica extract is a mixture of dried centella asiatica in a solvent (butylene glycol, ethanol, and distilled water mixed in a volume ratio of 40 to 60: 20 to 30: 20 to 30) in a weight ratio of 1: 1 to 30 Thereafter, centella extract can be prepared by vacuum low temperature extraction at 60 to 80° C. for 4 to 14 hours, preferably for 6 to 12 hours.

In addition, the Cheongho extract, licorice extract, neem leaf extract, comfrey leaf extract, green tea extract, purslane extract, rosemary extract, and dango elm bark extract can all be prepared in the same solvent and method as the centella asiatica extract described above.

The fermentation filtrate used for fermentation is a Bifida fermentation filtrate, a Lactobacillus fermentation filtrate, and a yeast fermentation filtrate in a weight ratio of 1: 0.5 to 2: 0.5 to 2, preferably 1: 0.8 to 1.5: 0.8 to 1.5 In terms of weight ratio, more preferably 1: 0.8 to 1.5: 0.8 to 1.2 weight ratio. At this time, if each fermentation filtrate exceeds the weight ratio, there may be a problem that fermentation of the plant extract mixture is not well done.

In addition, the Bifida Ferment Filtrate is an ICID-registered cosmetic ingredient, which is a material obtained by fermenting Bifidobacteria, an anaerobic lactic acid bacteria present in the digestive system, and filtering. Bifida fermented filtrate, as an ingredient that helps regenerate cells and strengthen the barrier, activates the supply of oxygen in the skin. By strengthening the skin barrier, it improves rough skin and holds the skin rhythm, improving skin tone, supplying moisture and nutrition, and giving a smooth application without stickiness. In addition, it is known to play a role in removing toxicity as it is used as a raw material for cosmetics.

In addition, the Lactobacillus fermentation filtrate is inoculated with Lactobacillus-based lactic acid bacteria in a plant extract mixture prepared by mixing the 9 types of plant extracts, and then 20 to 40°C, preferably 30 to 35°C under dark and anaerobic conditions. After fermentation at a temperature of at least 24 hours, preferably 24 to 36 hours, the fermented product is heated at 70 to 90° C. for 5 to 20 minutes to kill lactic acid bacteria, and then filtered. In addition, the inoculation of Bacillus-based lactic acid bacteria is preferably inoculated with 1×10 ⁵ to 1×10 ⁸ CFU, preferably 5×10 ⁵ to 5×10 ⁷ CFU in 1 mL of the plant extract mixture. In addition, as the Lactobacillus-based lactic acid bacteria, one selected from Lactobacillus bacteria, Streptococcus thermophilus, and Lactobacillus lactic can be used alone or in combination of two or more. have.

In addition, the yeast fermentation filtrate (Saccharomyces Ferment Filtrate) is a fermentation filtrate of Saccharomyces, a natural yeast used when making bread or beer, and is rich in amino acids, nucleic acids, peptides, minerals, and vitamins. Yeast fermentation filtrate contains NMF, a natural moisturizing factor, and contains 30% amino acids, 15% nucleic acids, and 30% peptides. The nucleic acid is an essential component of skin cell metabolism, reactivating skin cells and softening the skin. It is known to make it shiny.

Among the compositions used in the preparation of the present invention, the plant-extracted complex fermented product is inoculated with the fermentation filtrate in a weight ratio of 1: 0.10 to 0.20 weight ratio, preferably 1: 0.12 to 0.18 weight ratio, and then 36 to 36 at 30 to 37°C. It can be prepared by fermenting for 58 hours, preferably for 42 to 52 hours. The mixture may include 0.5 to 2.0% by weight of licorice and the remaining amount of the plant extract mixture, and preferably, 0.8 to 1.5% by weight and the remaining amount of the plant extract mixture, wherein the licorice is a fermentation filtrate It serves to promote the fermentation of the fruit.

The content of the plant-extracted complex fermented product in the cosmetic composition of the present invention is preferably 9.5 to 15.0% by weight, preferably 9.8 to 13.5% by weight, more preferably 10.0 to 12.5% by weight. At this time, if the content of the plant-extracted complex fermentation product is less than 9.5% by weight, there may be a problem in that atopy relief, skin trouble improvement, and/or skin soothing effect is insufficient, and if it exceeds 15% by weight, skin trouble may be caused. Good to use.

Next, among the components of the cosmetic composition of the present invention, the complex oil is used to increase the compatibility and miscibility between the active ingredient and the moisturizing agent ingredient of the plant-extracted complex fermentation product, and to increase the compatibility and miscibility between different compositions. Almond (Prunus Amygdalus Dulcis) oil, Marula seed (Sclerocarya Birrea Seed) oil, Argania Spinosa Kernel (Argania Spinosa Kernel) oil, Jojoba seed (Simmondsia Chinensis) oil, Rose hip seed (Rose hip seed) oil and at least one selected from lecithin May be used, preferably based on 100 parts by weight of sweet almond oil, 80 to 120 parts by weight of marula seed oil, 50 to 75 parts by weight of argan kernel oil, 50 to 75 parts by weight of jojoba seed oil, and 20 to 20 parts by weight of rosehip seed oil 40 parts by weight may be mixed and used, more preferably, based on 100 parts by weight of sweet almond oil, 90 to 115 parts by weight of marula seed oil, 55 to 70 parts by weight of argan kernel oil, 60 to 75 parts by weight of jojoba seed oil, 25 to 40 parts by weight of rosehip seed oil and 20 to 50 parts by weight of lecithin may be mixed and used.

In addition, the content of the composite oil may include 2.5 to 5.0% by weight, preferably 2.8 to 4.5% by weight, and more preferably 3.0 to 3.8% by weight of the total weight of the composition. At this time, if the content of the complex oil is less than 2.5% by weight, the active ingredient of the plant-extracted complex fermentation product does not dissolve well in the floral water, so skin improvement effects such as skin soothing effect may be reduced, and those exceeding 5.0% by weight may be used in excess. It is recommended to use within the above range because there may be a problem that the viscosity of the cosmetic is increased and the feeling of use is deteriorated due to the strong stickiness.

Next, among the composition components of the present invention, the moisturizing agent may use a general moisturizing component used as a cosmetic material, preferably glycerin, caprylic/capric triglyceride, butylene glycol, propane diol, Pentylene glycol, sodium vulinate (Sodium Levulinate), hydrogenated lecithin (Hydrogenated Lecithin), sodium hyaluronate (Sodium Hyaluronate) and ceramide NP (Ceramide NP) may include at least one selected from, Preferably selected from glycerin, caprylic/capric triglyceride, butylene glycol, propane diol, pentylene glycol, sodium bulinate, hydrogenated lecithin, sodium hyaluronate, and ceramide NP. It may contain 4 or more types, and even more preferably, all 9 types are mixed and used. When 9 types are mixed and used, it is preferable in terms of compatibility with other compositions while maximizing the moisturizing effect to use so as to have the composition shown in Table 1 below based on 100 parts by weight of glycerin.

glycerin 100 parts by weight Caprylic/Capric
Triglyceride 60 to 85 parts by weight, preferably 70 to 80 parts by weight Butylene Glycol 50 to 75 parts by weight, preferably 55 to 70 parts by weight Propanediol 40 to 60 parts by weight, preferably 43 to 55 parts by weight Pentylene glycol 20 to 45 parts by weight, preferably 25 to 40 parts by weight Sodium levulinate 10 to 30 parts by weight, preferably 12 to 25 parts by weight Hydrogenated Lecithin 5 to 25 parts by weight, preferably 7 to 18 parts by weight Sodium hyaluronate 1 to 10 parts by weight, preferably 2.5 to 8 parts by weight Ceramide NP 0.5 to 5 parts by weight, preferably 1.2 to 3.5 parts by weight

In addition, the amount of moisturizing agent is 13.0 to 20.0% by weight, preferably 15.0 to 18.0% by weight, more preferably 15.5 to 17.5% by weight of the total weight of the composition, and if the amount of the moisturizer is less than 13% by weight, moisturizing The effect may be insufficient, and if it is used in excess of 20% by weight, the viscosity of the cosmetic may be too high, and miscibility with other compositions may be sharply decreased.

Next, among the components of the composition of the present invention, the skin function improving agent may include ascorbyl glucoside , niacinamide, madecassoside, and/or adenine riboside. Here, the ascorbyl glucoside serves to improve and improve skin whitening. In addition, the niacinamide is used to obtain an effect of increasing skin whitening, relieving skin inflammation, and strengthening skin barrier by inhibiting the movement of melanin pigment. Madecassoside is used for promoting skin collagen synthesis, increasing skin elasticity, restructuring the extracellular matrix of the dermal layer, and soothing the skin.The adenosine is used to improve skin wrinkles, improve skin elasticity, and enhance cell proliferation in the skin dermal layer. To use. In the case of using all four types of skin function improving agents ascorbyl glucoside, niacinamide, madecassoside, and adenosine, based on 100 parts by weight of niacinamide, 10 to 30 parts by weight of ascorbyl glucoside, 1 to 5 parts by weight of madecassoside Parts and 0.5 to 4 parts by weight of adenosine, preferably based on 100 parts by weight of niacinamide, 12 to 25 parts by weight of ascorbyl glucoside, 1.5 to 4.0 parts by weight of madecassoside, and 1.0 to 3.0 parts by weight of adenosine are preferably used.

In addition, the amount of the skin function improving agent is preferably 1.5 to 4.0% by weight, preferably 1.7 to 3.5% by weight, more preferably 1.8 to 3.0% by weight of the total weight of the composition, and the skin function improving agent content is 1.5% by weight. If it is less than %, the amount of use is too small and the effect of using it may be insufficient, and if it is used in excess of 4% by weight, excessive use may cause skin trouble, so it is recommended to use it within the above range.

Next, the pH adjuster among the components of the cosmetic composition of the present invention is for controlling the overall pH of the composition, and if it does not affect the skin and does not degrade the effect of the cosmetic composition of the present invention, a pH adjuster used in the art can be used. , For example, a preferred example is Sodium Anisate. In addition, the content of the pH adjusting agent is 0.1 to 1.5% by weight, preferably 0.4 to 1.2% by weight, more preferably 0.5 to 1.1% by weight of the total weight of the composition is suitable for maintaining an appropriate pH.

Next, among the components of the cosmetic composition of the present invention, the surfactant is well dissolved in floral water along with the complex oil, and serves to increase compatibility and miscibility between the compositions, and does not adversely affect the present invention. Within the range, general surfactants used in the art may be used, and preferably at least one selected from dehydroxanthan gum and screllotium gum may be included, and more preferably dehydroxanthan gum and screl Rotium gum can be mixed and used in a weight ratio of 1: 2.0 to 3.0.

The content of the surfactant may include 0.10 to 0.70% by weight, preferably 0.20 to 0.60% by weight, and more preferably 0.25 to 0.55% by weight of the total weight of the cosmetic composition, in which case, the content of the surfactant is 0.10% by weight If the amount is less than %, the amount of the surfactant may be insufficient, and the use in excess of 0.70% by weight may cause excessive use and rather may cause skin problems, so it is recommended to use within the above range.

Arginine among the components of the cosmetic composition of the present invention plays a role in improving skin whitening, and the amount of arginine used may include 0.05 to 0.30% by weight, preferably 0.15 to 0.30% by weight of the total weight of the cosmetic composition.

Next, among the components of the cosmetic composition of the present invention, the color expressing agent is used for cosmetic products to express a white-based color to induce aesthetics and improve product quality, and includes ascorbyl glucoside and shea butter, preferably It may contain 1.5 to 4.0% by weight of shea butter and the balance of ascorbyl glucoside.

Next, preservatives, flavors, antioxidants, and the like may be included as other additives among the composition components of the present invention.

The preservative may be a general preservative that is acceptable as a cosmetic ingredient, and preferably, glyceryl caprylate is 0.1 to 0.5% by weight, preferably 0.15 to 0.45% by weight, more preferably 0.15 to 0.40% by weight of the total weight of the composition. It is better to use %.

In addition, the fragrance may be used in a small amount of common fragrances used in the art, preferably 0.3% by weight or less, more preferably about 0.05 to 0.25% by weight of the total weight of the composition.

In addition, the antioxidant is used to improve the storage stability of the atopic dermatitis improving agent together with a preservative, and for example, ascorbic acid or a salt thereof, ascorbic acid ester, erythorbic acid, and the like may be used. In this case, the salt is an alkali metal salt such as sodium and potassium; Alkaline earth metal salts such as calcium and magnesium; Or ammonia salt, etc. are mentioned. In addition, the amount of antioxidant used is preferably 0.01 to 3% by weight, preferably 0.01 to 1.5% by weight, based on the total weight of the composition.

Next, among the components of the cosmetic composition of the present invention, the floral water (flower water) serves as a solvent and skin improvement effect in the composition, and accounts for the remaining weight% of the total weight of the composition in addition to the compositions described above. In the present invention, the floral water includes centellar leaf number, lavender flower number, port marigold flower number, rosemary water, and green tea water, and preferably, based on 100 parts by weight of centella leaf number, 0.05 to 5 parts by weight of lavender flower number, pot 0.05 to 5 parts by weight of marigold flower water, 0.05 to 3 parts by weight of rosemary water, and 0.05 to 3 parts by weight of green tea water, more preferably 0.05 to 2 parts by weight of lavender flower water, based on 100 parts by weight of centella leaves It may contain 0.05 to 2 parts by weight of gold flower water, 0.05 to 1 part by weight of rosemary water, and 0.05 to 1 part by weight of green tea water.

The floral water is extracted by a low-temperature critical extraction method, and a preferred example is, for example, dried centella leaves, lavender flowers, pot marigold flowers, rosemary leaves and/or rosemary leaf stems, green tea leaves, or a mixture of them at ultra high speed vacuum low temperature. 1 step of mixing distilled water and 1: 1 to 30 weight ratio, preferably 1: 3 to 25 weight ratio in the extractor, and then extracting for 8 to 12 hours at about 60 to 80 ℃; It may be prepared by performing a process including; step 1 of putting the extract of step 1 into a vacuum low-temperature extractor, and concentrating and cooling it. In addition, the floral water may be diluted when used.

The cosmetic composition (and/or cosmetic) of the present invention prepared by the above manufacturing method using the above cosmetic composition has excellent skin soothing effect and skin moisturizing power, does not cause skin troubles such as allergies, and has a skin improvement effect. , It has the effect of improving atopic dermatitis. The cosmetic composition of the present invention can be applied as a material as a skin improvement ointment, as well as cosmetics of various formulations.

Hereinafter, the present invention will be described in more detail through examples, but the following examples do not limit the scope of the present invention, which should be interpreted to aid understanding of the present invention.

[Example]

Preparation Example 1: Preparation of floral water (flower water)

(1) Manufacture of Centella asiatica

Centella Asiatica Leaf (Centella Asiatica Leaf) was added to distilled water at a weight ratio of 1:3 in an ultra-high-speed vacuum low-temperature extractor (Gyeongseo Machinery, COSMOS-660), and extracted at about 80° C. for 10 hours to obtain Centella Asiatica Leaf floral water. This floral water was again put into the vacuum low-temperature extractor, concentrated, cooled, and stored in a refrigerator (about 3° C.).

(2) Preparation of lavender water

Lavandula Angustifolia flowers, leaves, and distilled water dried in an ultra-high-speed vacuum extractor (COSMOS-660) were added in a 1:1 weight ratio, and extracted at about 75℃ to 77℃ for 10 hours to obtain lavender floral water. . This floral water was again put into the vacuum low-temperature extractor, and concentrated so that the lavender plant extract component was 30-50% by weight. This was allowed to cool and stored refrigerated (about 3°C).

(3) Manufacture of pot marigold flower water

Pot Marigold (Calendula officinalis) flowers dried in an ultra-high-speed vacuum low-temperature extractor (Gyeongseo Machinery, COSMOS-660) were added to distilled water at a weight ratio of 1:20, and extracted at about 75°C for 5 hours to obtain portmarigold floral water. This floral water was again put into the vacuum low-temperature extractor, concentrated, cooled, and stored in a refrigerator (about 3° C.).

(4) Preparation of rosemary water

The leaves and stems of rosemary (Rosmarinus Officinalis) dried in an ultra-high-speed vacuum low-temperature extractor (Gyeongseo Machinery, COSMOS-660) were added to distilled water at a weight ratio of about 1:20, and extracted at about 75° C. for 5 hours to obtain rosemary floral water. This floral water was again put into the vacuum low-temperature extractor, concentrated, cooled, and stored in a refrigerator (about 3° C.).

(5) Preparation of green tea water

Green tea leaves (Camellia Sinensis Leaf) dried in an ultra-high-speed vacuum low-temperature extractor (Gyeongseo Machinery, COSMOS-660) were added to distilled water at a weight ratio of 1:20, and extracted at about 75° C. for 5 hours to obtain rosemary floral water. This floral water was again put into the vacuum low-temperature extractor, concentrated, cooled, and stored in a refrigerator (about 3° C.).

(6) Manufacture of mixed floral water (flower water)

Floral water was prepared by mixing 0.15 parts by weight of lavender flower water, 0.15 parts by weight of pot marigold flower water, 0.15 parts by weight of rosemary water, and 0.15 parts by weight of green tea water with respect to 100 parts by weight of centella leaf water.

Preparation Example 2-1: Preparation of plant-extracted complex fermented product

(1) Preparation of plant extract mixture

Put dry centella in an ultra-high-speed vacuum low-temperature extractor (Kyungseo Machinery, COSMOS-660) in a solvent of butylene glycol/ethanol/distilled water (50/25/25 volume ratio) at a weight ratio of 1:5, and extract at about 75℃ for 12 hours. Centella asiatica extract was obtained. The obtained extract was distilled to recover an organic solvent. The concentration of the extract was about 5%. It was concentrated again to remove about 50% of moisture in the extract. The centella asiatica extract thus obtained was stored refrigerated (about 3°C).

Cheongho, licorice, neem leaves, comfrey leaves, green tea, purslane, rosemary, and dandelion elm bark are prepared in the same manner as the centella asiatica extract. And to prepare each of the bark extract of the elm.

Next, based on 100 parts by weight of centella asiatica extract, 75 parts by weight of Cheongho extract, 75 parts by weight of licorice extract, 45 parts by weight of neem leaf extract, 45 parts by weight of comfrey leaf extract, 31.5 parts by weight of green tea extract, 31.5 parts by weight of purslane extract, rosemary A plant extract mixture was prepared by mixing 31.5 parts by weight of the extract and 31.5 parts by weight of the dandelion bark extract.

(2) Preparation of fermentation filtrate

Bifida fermentation filtrate, Lactobacillus fermentation filtrate, and yeast fermentation filtrate were prepared in a 1:1 weight ratio.

At this time, the Bifida fermentation filtrate and yeast fermentation filtrate (Saccharomyces yeast fermentation filtrate) were purchased and used.

In addition, the Lactobacillus fermentation filtrate was inoculated with 1.2×10 ⁶ to 1.5×10 ⁶ CFU of Lactobacillus bacteria in 1 mL of the plant extract mixture, and then about 30 under dark and anaerobic conditions. After fermentation at a temperature of ~ 32°C for 26 hours, the fermented product was heated at 85°C for 15 minutes to kill lactic acid bacteria, and then filtered to obtain a filtrate.

(3) Preparation of plant-extracted complex fermented products

After inoculating 16.1 parts by weight of the fermentation filtrate to 100 parts by weight of the plant extract mixture, fermentation was performed at 35 to 38° C. for 48 hours to prepare a plant-extracted complex fermentation product.

Preparation Example 2-2: Preparation of plant-extracted complex fermented product

(1) Preparation of plant extract mixture

A plant extract mixture having the same method, composition, and composition ratio as in Preparation Example 2-1 was prepared.

(2) Preparation of mixture

A mixture was prepared by mixing 1% by weight of licorice (dipotassium glycyrrhizate) and 99% by weight of the plant extract mixture.

(3) Preparation of fermentation filtrate

A fermentation filtrate having the same method, composition, and composition ratio as in Preparation Example 2-1 was prepared.

(4) Preparation of plant-extracted complex fermented products

After inoculating 16.1 parts by weight of the fermentation filtrate to 100 parts by weight of a mixture of the plant extract mixture and licorice acid, it was fermented at 35 to 38° C. for 48 hours to prepare a plant extract complex fermented product.

Example 1: Preparation of a cosmetic material excellent in skin whitening improvement and soothing effect.

The plant-extracted complex fermented product of Preparation Example 2-1, oil, moisturizer, skin function improving agent, surfactant, pH adjuster, and other additives (preservatives, fragrances) were added to the flower water (floral water) of Preparation Example 1 as shown in Table 3 below. After the composition ratio was added, it was heated to about 70°C.

Next, a cosmetic was prepared by stirring and mixing for about 10 minutes with an ultra-high-speed disperser (ultimizer).

At this time, the components and component ratios of the moisturizing agent, skin function improving agent, surfactant, pH adjuster, and other additives are as shown in Table 2 below.

division Kinds Parts by weight division Kinds Parts by weight oil Sweet Almond Oil 100 Skin function improvement agent Niacinamide 100 Marula Seed Oil 100 Madecassoside 2.5 Argan kernel oil 66 Adenosine 2 Jojoba seed oil 66 Surfactants Dehydroxanthan gum 1:2.5
Weight ratio Rosehip seed oil 33 Sclerotium sword Moisturizer glycerin 100 pH adjuster Sodium anisate - Caprylic/Capric Triglyceride 74.8 antiseptic Glycerylcaprylate - Butylene Glycol 62.4 Spices Fragrance(CharmZone) - Propanediol 49.9 - - - Pentylene glycol 31.2 Sodium levulinate 16.6 Hydrogenated Lecithin 10.4 Sodium hyaluronate 4.2 Ceramide NP 2.1

Examples 2 to 4 and Comparative Examples 1 to 4

Same as in Example 1 Cosmetics are prepared using flower water (floral water), plant-extracted complex fermented product, oil, moisturizer, skin function improving agent, surfactant, pH adjuster and other additives (preservatives, fragrances), but the composition shown in Tables 3 and 4 below. And prepared to have a composition ratio to prepare a cosmetic, respectively, Examples 2 to 4 and Comparative Examples 1 to 4 were carried out, respectively.

Example 5

Same as in Example 1 Cosmetics are prepared using flower water (floral water), oil, moisturizer, skin function improving agent, surfactant, pH adjuster, and other additives (preservatives, fragrances), but instead of the plant-extracted complex fermented product of Preparation Example 2-1, Preparation Example 2 Example 5 was carried out by using the plant-extracted fermented product of -2, and further using arginine and a color expressing agent to prepare a cosmetic. At this time, the color expressing agent contains 2% by weight of shea butter and the remaining amount of ascorbyl glucoside. In addition, the skin function improving agent was used containing 13.5 parts by weight of ascorbyl glucoside, 2.5 parts by weight of madecassoside, and 2.0 parts by weight of adenosine, based on 100 parts by weight of niacinamide. The prepared cosmetic was a light blue color.

Classification (% by weight) Example 1 Example 2 Example 3 Example 4 Example 5 Plant-derived complex fermented product 10.82 9.80 13.50 10.82 10.82 Compound oil 3.3 3.3 3.3 2.8 2.8 Moisturizer 16.91 16.91 16.91 16.91 16.91 Skin function improvement agent 2.09 2.09 2.09 2.09 2.09 Surfactants 0.35 0.35 0.35 0.35 0.35 Arginine - - - - 0.20 Color developer - - - - 0.52 pH adjuster 0.80 0.92 0.73 0.82 0.82 Other additives antiseptic 0.3 0.3 0.3 0.3 0.3 Spices 0.2 0.2 0.2 0.2 0.2 Number of flowers 100% by weight remaining

Classification (% by weight) Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Plant-derived complex fermented product 8.5 16.5 10.82 10.82 Compound oil 3.3 3.3 1.5 6.0 Moisturizer 16.91 16.91 16.91 16.91 Skin function improvement agent 2.09 2.09 2.09 2.09 Surfactants 0.35 0.35 0.35 0.35 pH adjuster 0.95 0.70 0.80 0.80 Other additives antiseptic 0.3 0.3 0.3 0.3 Spices 0.2 0.2 0.2 0.2 Number of flowers 100% by weight remaining

Experimental Example 1: Evaluation of skin soothing effect

(1) Evaluation criteria

Commissioned by the Korea Research Institute of Dermatology (test period: January 18, 2019 to February 25, 2019), the skin soothing effect of cosmetics was tested.

Among adult women aged 20 to 60 years, 20 women (average age 44.90 years old) were selected according to the selection criteria shown in Table 5 below, and the subject's facial area was selected as the test site. In addition, during the test period, after washing your face twice a day in the morning and in the evening, the test substance, cosmetic, was evenly spread over the face area for absorption.Eye cream, whitening cream, and anti-aging that may affect the test result during the test period The use of functional cosmetics such as creams and exfoliating agents was prohibited at all.

The evaluation method is shown in Table 5 below, and the results of the questionnaire survey on the skin condition of the test subjects (20 persons) are shown in Table 6 below.

Test subject
Selection criteria (1) A person who voluntarily fills out and signs a consent form after having been sufficiently explained about the matters to be informed to the test subject from the person in charge of the study or from the person who has been delegated by the test director
(2) Adult females aged 20 to 60 years old, healthy people with no major or chronic physical diseases including skin diseases
(3) Those who can follow up during the test period Criteria for exclusion of test subject selection (1) Pregnant or lactating women and women who are likely to become pregnant
(2) Those who use steroid-containing skin external preparations for more than 1 month to treat skin diseases
(3) Those who have not passed 6 months after taking the same exam
(4) People with sensitive or irritable skin
(5) Persons with skin abnormalities such as spots, acne, erythema, and expansion of capillaries on the test site
(6) Those who received treatment at the test site within 6 months before the start of the study
(7) Others who are deemed unsuitable for the test at the judgment of the test manager Test subject dropout criteria (1) In case of adverse events such as itching or erythema at the test site
(2) In case the test subject has difficulty in evaluating the results due to medical treatment, application of other products, excessive UV exposure, excessive drinking and smoking during the test process
(3) In case it is difficult for the test subject to follow up due to personal circumstances during the test process
(4) When the test subject violates the method of use or schedule without special reason

Test site Measured after resting in a constant temperature and humidity room (temperature: 22±2℃, humidity: 50±5%) for 30 minutes after washing your face with the same face wash at the Korea Research Institute of Dermatology. Measure Spectrophotometer CM-2600D, Konica Minolta, Japan and anterior vision imaging system VISIA-CA (VISIA Complexion Analysis, Canfield Scientific, Inc., USA) were applied. The same test person in charge of the spectrophotometer measured the left cheek area of all subjects three times in a row, and the average value was calculated and used for analysis. The measured value of the spectrophotometer consists of three factors: L*, a*, and b*, L* value is brightness, a* value is redness, and b* value is yellow. Represents. As a value to measure the skin soothing effect of the test substance, a* value indicating the redness of the skin was used for analysis, and as the measured value decreases compared to before use of the test substance, it means that there is a skin soothing effect. In the anterior eye imaging system VISIA-CA, all subjects' facial areas were measured by the same test person at the same position with uniform illumination for consistent imaging. In order to secure the hairline of the subject's forehead, the hair was fixed with a hairband, and then the same top gown was worn and seated on a chair. . Instrument measurements were made before and after 2 weeks of use of the test substance. Poll A questionnaire survey was conducted on the characteristics of the test subject's general skin condition, skin condition before and after use of the test substance, and feeling of use of the test substance, and one question on the characteristics of the general skin condition and two questions on the skin condition before and after use of the test substance were selected. It was investigated using. Also, in order to examine the feeling of use of the test substance, 4 questions were conducted using the satisfaction/dissatisfaction option.

(2) Skin soothing evaluation result

The results of evaluating the skin soothing effect before and after 2 weeks of use of the test substance using a spectrophotometer and an anterior ocular imaging system VISIA-CA are shown in Table 7 below, and a ^* value change and a ^* value improvement rate (% ) Is shown in FIG. 1.

And, the photographs of the skin soothing effect by the anterior eye photographing system VISIA-CA for Example 1 are shown in FIGS. 2A to 2F, respectively.

division a ^* value change a ^* value improvement rate (%) a ^* value statistical analysis* Average Standard Deviation Example 1 Before use 12.02 1.40 - - 2 weeks after use 11.51 0.99 4.28% 0.013 a ^* value improvement rate (%) = [measured value after use-measured value before use]/measured value before use × 100(%)
*p <0.05 **p <0.01 ***p <0.001: p-value is measured by paired t-test

As a result of analyzing the degree of improvement of the skin soothing effect on the left cheek area using a spectrophotometer and an anterior eye imaging system VISIA-CA, in the case of Example 1, the a* value representing the redness of the skin was used for 2 weeks compared to before using the test substance. The change was decreased by 4.28%. In addition, it was statistically significant after 2 weeks of use compared to before use of the test substance (*p<0.05), confirming that the test substance is a product that helps the skin soothing effect.

Experimental Example 2: Evaluation of adverse skin reactions and feeling of use

Simultaneously with Experimental Example 1, after 2 weeks of using the cosmetics of Examples and Comparative Examples, questionnaire evaluation was conducted on whether abnormal skin reactions occurred and feeling of use.

The criteria for selecting test subjects are shown in Table 5, and the questionnaire evaluation on the skin condition of the test subjects before the evaluation for 20 subjects is shown in Table 8 below.

In addition, in the evaluation of adverse skin reactions and the feeling of use, each of 20 test subjects used the cosmetics of Examples 1 to 4 and Comparative Examples 1 to 4 for 2 weeks, and then the survey results are shown in Tables 9 to 11 below. After using one sample for 2 weeks, after having a rest period for 1 week, the next sample was used.

Adverse reaction
evaluation Observation of skin abnormalities such as erythema, edema, scaling, itching, stinging, burning, stiffness, and prickling at the test site When an adverse skin reaction occurred, the grade was indicated and the result was written. In addition, a questionnaire survey on adverse skin reactions was conducted with the subjects. Poll A questionnaire survey was conducted on the characteristics of the test subject's general skin condition, skin condition before and after use of the test substance, and feeling of use of the test substance, and one question on the characteristics of the general skin condition and two questions on the skin condition before and after use of the test substance were selected. It was investigated using. Also, in order to examine the feeling of use of the test substance, 4 questions were conducted using the choice of satisfaction/dissatisfaction.

Questionnaire Among test subjects
percentage(%) Skin type Intelligence 5 Normal (normal skin) 15 Complexity (T zone intelligence, U zone intelligence) 55 inattention 25 Sensitiveness 0 Skin condition before test Questionnaire Among test subjects
percentage(%) My skin is red or not sensitive Not at all 20 Not like that 45 is average 35 Yes 0 it really is 0 The skin is moist and moist Not at all 25 Not like that 30 is average 45 Yes 0 it really is 0

division Percentage (%) after 2 weeks of use Feeling question answer Example 1 Example 2 Example 3 Example 4 Example 5 The texture of the formulation Satisfaction 95 95 90 90 90 dissatisfaction 5 5 10 10 10 Sedation Satisfaction 100 100 95 100 100 dissatisfaction 0 0 5 0 0 Moisturizing Satisfaction 100 100 100 5 100 dissatisfaction 0 0 0 5 0 Overall satisfaction Satisfaction 100 100 95 95 100 dissatisfaction 0 0 5 5 0 skin
More than
reaction
evaluation question answer Example 1 Example 2 Example 3 Example 4 Example 5 The red and sensitive skin feels soothing. Not at all 0 0 0 0 0 Not like that 0 0 0 0 0 is average 15 30 10 20 5 Yes 75 65 80 70 85 it really is 10 5 10 10 10 The skin seems to be moist and moisturized. Not at all 0 0 0 0 0 Not like that 0 0 0 0 0 is average 60 65 40 75 50 Yes 40 35 65 25 45 it really is 0 0 5 0 0

division Percentage (%) after 2 weeks of use Feeling question answer Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 The texture of the formulation Satisfaction 90 85 100 75 dissatisfaction 10 15 0 25 Sedation Satisfaction 85 90 100 85 dissatisfaction 15 10 0 15 Moisturizing Satisfaction 100 95 80 95 dissatisfaction 0 5 20 5 Overall satisfaction Satisfaction 90 90 85 80 dissatisfaction 10 10 15 20 Skin adverse reaction evaluation question answer Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 The red and sensitive skin feels soothing. Not at all 0 5 0 0 Not like that 20 10 5 0 is average 25 15 35 60 Yes 60 70 60 40 it really is 0 0 0 0 The skin seems to be moist and moisturized. Not at all 0 0 0 0 Not like that 0 0 5 0 is average 55 40 70 60 Yes 45 60 25 25 it really is 0 0 0 15

Looking at Tables 10 and 11, it was confirmed that in the case of Examples 1 to 5, both had excellent feeling of use and no adverse skin reactions. On the other hand, Comparative Example 1 is a problem in which the soothing sensation in the feeling of use is somewhat inferior in the case of Comparative Example 1 in which the plant-extracted complex fermentation product is used in an amount of less than 9.5% by weight, compared to Examples 1 and 2 There was. In addition, Comparative Example 2 in which the plant-extracted complex fermentation product was used in excess of 15% by weight had a problem that a tester who caused skin troubles in the skin abnormality evaluation occurred. In addition, there was a problem that the moisturizing power of using the complex oil in less than 2.5% by weight was poor, so the feeling of use was somewhat insufficient, and the result of skin abnormality evaluation was inferior. And, Comparative Example 3, in which the composite oil was used in excess of 5% by weight, exhibited a result that the viscosity was too high, so that the feeling of use was not very good.

Experimental Example 3: Human patch experiment

Separately from Experimental Example 1, a human patch experiment of the cosmetic composition of Example 1 was performed by requesting the Seowon Global Skin Clinical Center (experiment period 5 weeks).

In the test, the IQ ultra chamber containing the sample was attached to the skin of the subject's arm, etc., removed after 24 hours, and the results were read after 30 minutes and 24 hours. The test subjects were adult men and women who satisfy the test subject selection criteria in Table 5 (average age 23.6 years), and the final 32 test subjects were selected.

The skin characteristics of the test subject are shown in Table 12 below.

Skin characteristics ratio(%) Skin characteristics ratio(%) Normal skin 34.37 Metal allergy 9.37 Oily skin 25.00 Other allergies 12.50 Dry skin 37.50 Irritation sensitivity 31.25 Very dry skin 3.12 Susceptibility to burning 25.00 Sunlight allergy 3.12 - -

The average skin reactivity was measured based on the following equation, and the skin irritation criterion was finally determined based on the following Table 13, and the evaluation results are shown in Table 14 below.

[Equation 1]

Grade Mean Score No irritation 0.0 to 0.9 Light stimulation 1.0 to 2.9 Heavy stimulation 3.0 to 4.9 Strong stimulation 5.0 ~

division Average skin reactivity Judgment Example 1 0.52 No irritation Example 2 0.52 No irritation Example 3 0.86 No irritation Example 4 0.38 No irritation Example 5 0.48 No irritation Comparative Example 1 0.52 No irritation Comparative Example 2 1.64 Light stimulation Comparative Example 3 0.38 No irritation Comparative Example 4 1.24 Light stimulation

As a result of the human patch test, the average skin reactivity of Examples 1 to 5 was shown as no irritation. However, in Comparative Example 2 in which the content of the plant-extracted complex fermentation product was excessive, and Comparative Example 4 in which the complex oil content was used in excess of 5% by weight and the feeling of being too sticky, there was a test subject that caused skin troubles.

Experimental Example 4: Investigation of atopic skin improvement effect

The clinical evaluation of the atopic skin improvement effect of the cosmetics of Examples 1 and 5 was measured. The following experiment was conducted on 15 children with atopic skin who visited the hospital and were treated. The test sample was divided into right and left sides by a double-blinded test, and applied to the whole body, but the use of other moisturizers and cosmetics that may affect the effect of the test sample as far as possible was prohibited. The effects of 1 week, 2 weeks, 3 weeks, and 4 weeks after application of the test sample were calculated according to Equation 2 by SCORAD (SCORing Atopic Dermatitis) measurement method, and the improvement rate was calculated according to Equation 3 And the results are shown in Table 15 below.

The control group in Table 15 did not include only the plant-extracted complex fermented product in Example 1, and other compositions were used in the same amount.

[Equation 2]

Improvement effect value = (based on accuracy/5) + (based on strength/7) + (based on subjective)

In Equation 2, the extent criteria are area = skin area/100, and the intensity criteria are erythema (1 to 3 points), edema (1 to 3 points), effusion (1 to 3 points), Skin peeling (1 to 3 points), lichenification degree (1 to 3 points) and dryness (1 to 3 points), and the subjective criteria are itching (1 to 10 points) and insomnia (1 to 10 points).

[Equation 3]

Improvement rate (%) = (0 week improvement effect value-measured week's improvement effect value)/ (0 week improvement effect value) × 100 (%)

division 0 weeks 1 week 2 weeks 3 weeks 4 weeks Example 1 shame 72.5 58.8 53.4 39.1 28.8 Improvement rate 0% 18.9% 26.3% 46.1% 60.3% Example 5 shame 72.5 58.0 52.7 39.4 29.5 Improvement rate 0% 20.0% 27.3% 45.66% 59.31% Control shame 72.5 60.7 54.8 49.0 38.5 Improvement rate 0% 16.3% 24.4% 32.4% 46.9%

Looking at the measurement results in Table 15, when comparing Examples 1 and 5 with the control group, when atopic dermatitis of atopic dermatitis patients was treated with a cosmetic containing the plant-extracted complex fermented product of the present invention, the atopic dermatitis improvement effect was observed. It was confirmed to increase.

Experimental Example 5: Analysis of the inhibitory effect of tyrosinase activity

Examples 1 and 5 Analysis of the inhibitory effect of tyrosinase activity of cosmetics was performed. At this time, arbutin, which is well known as a whitening agent, was used as a comparative sample.

Tyrosinase is an enzyme that promotes the oxidation process of a substance called tyrosine in vivo to produce melanin. Tyrosinase was isolated and purified from mushrooms and was purchased from Sigma and used. L-tyrosine as a substrate was dissolved in 0.05 M sodium phosphate buffer (pH 6.8) to make a 0.1 mg/ml solution, and acupuncture extract was dissolved in 0.05 M sodium phosphate buffer (pH 6.8) to an appropriate concentration. . 0.5 ml of L-tyrosine solution was placed in a test tube, 0.5 ml of acupuncture extract sample solution was added thereto, and left in a 37°C incubator for 10 minutes. Then, 200 unit/ml tyrosinase 0.5 ml was added and reacted at 37°C for 10 minutes. The test tube containing the reaction solution was placed on ice to rapidly cool to stop the reaction, and then the absorbance at a wavelength of 475 nm was measured with a spectrophotometer. As a control, 0.5 ml of a buffer solution was added instead of acupuncture extract. At this time, the activity inhibition rate (%) for tyrosinase was calculated by Equation 1 below, and the IC ₅₀ (ug/ml) value when the tyrosinase activity was inhibited by 50% was measured, respectively. The results are shown in Table 16 below.

[Equation 4]

Inhibition rate of tyrosinase activity (IC ₅₀ , %) = 100 -[(absorbance in control group/absorbance in control group)]×100

division IC ₅₀ (ug/ml) (concentration required for 50% inhibition of tyrosinase activity) Arbutin 197.8 Example 1 215.7 Example 5 180.3

Looking at Table 16, the concentration of arbutin that inhibits tyrosinase activity by 50% was 197.8 ug/ml, and Example 1 showed a slightly higher concentration than arbutin. On the other hand, Example 5 showed an IC ₅₀ lower than that of arbutin, and it was confirmed that it has a superior tyrosinase activity inhibitory effect than arbutin.

Experimental Example 6: Analysis of melanin production inhibitory effect using B16F10 melanin-forming cells

An experiment to confirm the inhibitory effect of melanin production on B16F10 melanin-forming cells was performed, and at this time, arbutin, which is well known as a whitening agent, was used as a comparative sample.

The B16F10 melanin-forming cells used in the experimental examples were mouse-derived cell bacteria, and were pre-sold from ATCC (American Type Culture Collection) and used. B16F10 melanin-forming cells were dispensed in a 6-well plate at a concentration of 2×10 ⁵ CFU per well, and after attaching the cells, dispensed at a concentration that did not cause toxicity, treated with the cosmetic of Example 5 and cultured for 72 hours. . After 72 hours of culture, the cells were detached with trypsin-EDTA, the number of cells was measured, and then the cells were recovered by centrifugation. The quantification of intracellular melanin was performed by modifying the method of Rotan (Cancer Res., 40: 3345-3350, 1980). After washing the cell pellet with PBS once, 1 ml of a homogenization buffer (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added and vortexed for 5 minutes to disrupt the cells. After dissolving the extracted melanin by adding 1N NaOH (10% DMSO) to the cell filtrate obtained by centrifugation (3,000 rpm, 10 minutes), the absorbance of melanin was measured at 405 nm with a microplate reader, and then the melanin was quantified. The melanin production inhibition rate (%) was measured. The melanin production inhibition rate (%) of B16F10 melanin forming cells was calculated by the following equation, and the results are shown in Table 17.

[Equation 5]

Melanogenesis inhibition rate (%) = [(A-B)/A]×100%

A: the amount of melanin in the well to which the sample is not added, B: the amount of melanin in the well to which the sample is added

Treatment agent/treatment concentration (ug/ml) Melanogenesis inhibition rate (% of control) Example 5/1 ug/ml 0.2 Example 5/10 ug/ml 15.3 Example 5/100 ug/ml 37.5 Example 5/300 ug/ml 49.7 500 ug/ml arbutin 48.9

Table 17 shows that when using about 300 ug/ml of sparring rice mon, the cosmetic of Example 5, it can be seen that it shows almost the same or improved effect as when treated with 500 ug/ml of arbutin, through which, according to the present invention, It was confirmed that the cosmetic had an excellent whitening effect.

Claims (8)

Plant-extracted complex fermentation product 9.5 to 15.0% by weight, complex oil 2.5 to 5.0% by weight, moisturizing agent 15.0 to 18.0% by weight, skin function improving agent 1.5 to 4.0% by weight, pH adjusting agent 0.1 to 1.5% by weight, surfactant 0.10 to 0.70% by weight , Arginine (Arginine) 0.05 ~ 0.3% by weight, containing 0.1 ~ 1.0% by weight of color developer and the balance of floral water,
The plant-extracted complex fermented product is a fermented product obtained by inoculating and fermenting a fermented filtrate in a mixture containing 0.5 to 2.0% by weight of licorice acid and the remaining amount of the plant extract mixture in a weight ratio of 1: 0.10 to 0.20,
The complex oil is sweet almond (Prunus Amygdalus Dulcis) oil, Marula seed (Sclerocarya Birrea Seed) oil, argan kernel (Argania Spinosa Kernel) oil, jojoba seed (Simmondsia Chinensis) oil, Rose hip seed (Rose hip seed) oil and lecithin A cosmetic composition having a skin whitening improvement and soothing effect comprising (lecithin). Plant-extracted complex fermentation product 9.5 to 15.0% by weight, complex oil 2.5 to 5.0% by weight, moisturizing agent 15.0 to 18.0% by weight, skin function improving agent 1.5 to 4.0% by weight, pH adjusting agent 0.1 to 1.5% by weight, surfactant 0.10 to 0.70% by weight , Arginine (Arginine) 0.05 ~ 0.3% by weight, containing 0.1 ~ 1.0% by weight of color developer and the balance of floral water,
The plant-extracted complex fermented product is a fermented product obtained by inoculating and fermenting a fermented filtrate in a mixture containing 0.5 to 2.0% by weight of licorice acid and the remaining amount of the plant extract mixture in a weight ratio of 1: 0.10 to 0.20,
The plant extract mixture is based on 100 parts by weight of centella asiatica extract, 60 to 100 parts by weight of blueberry extract, 60 to 100 parts by weight of licorice extract, 30 to 70 parts by weight of neem leaf extract, 30 to 70 parts by weight of comfrey leaf extract, 15 to 70 parts by weight of green tea extract 50 parts by weight, 15 to 50 parts by weight of purslane extract, 10 to 45 parts by weight of rosemary extract, and 10 to 45 parts by weight of sugar elm bark extract,
The fermentation filtrate is a cosmetic composition having a skin whitening improvement and soothing effect, characterized in that it comprises a Bifida fermentation filtrate, a Lactobacillus fermentation filtrate, and a yeast fermentation filtrate. delete The method of claim 1, wherein the skin function improving agent comprises at least one selected from ascorbyl glucoside, niacinamide, madecassoside, and adenine riboside. A cosmetic composition having a skin whitening improvement and soothing effect. The method of claim 1, wherein the moisturizing agent is glycerin, caprylic/capric triglyceride, butylene glycol, propane diol, pentylene glycol, sodium vulinate, hydrogenated lecithin ( Hydrogenated Lecithin), sodium hyaluronate (Sodium Hyaluronate) and ceramide NP (Ceramide NP), characterized in that it comprises at least one selected from the skin whitening improvement and soothing effect cosmetic composition. The cosmetic composition of claim 1, wherein the color expressing agent comprises ascorbyl glucoside and shea butter. It contains the cosmetic composition of any one selected from claim 1, claim 2 or claim 4 to claim 6,
Cosmetics having a skin whitening improvement and soothing effect, characterized in that it has a white color. After heating the cosmetic composition of any one of claims 1, 2, or 4 to 6 to 50 to 80°C, it includes a step of stirring and mixing with an ultra-high pressure disperser. And a method for producing a cosmetic having a soothing effect.

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