KR102340001B1 - Cosmetics material having inhibitor for NO activator and tyrosinase activator, Revitalizing cosmetics containing the same and Manufacturing method thereof - Google Patents

Abstract

The present invention relates to a cosmetic using a natural resource material and functional cosmetics comprising the same, and more specifically, to an invention capable of providing functional cosmetics which have an anti-inflammatory effect and can prevent skin pigmentation by applying a complex extract containing extracts derived from a natural plant as a cosmetic, and cosmetics using the same.

Description

Cosmetics having NO activity inhibitory activity and tyrosinase activity inhibitory activity, revitalizing cosmetics containing the same, and manufacturing method thereof {Cosmetics material having inhibitor for NO activator and tyrosinase activator, Revitalizing cosmetics containing the same and Manufacturing method thereof}

The present invention is excellent in inhibiting NO active ingredients that cause inflammation in the skin, etc., while having excellent NO activity inhibition ability that effectively restores stressed and tired skin, reducing or inhibiting pigmentation expression through inhibition of tyrosinase enzyme activity It relates to a cosmetic derived from a natural plant extract capable of alleviating or preventing pigmentation, such as, a revitalizing cosmetic using the same, and a method for manufacturing the same.

The inflammatory response is a defense mechanism of the human body against external stimuli to maintain homeostasis. It occurs through external stimuli such as LPS (lipopolysaccharide) derived from microorganisms and internal stimuli such as arachidonic acid.

LPS is a substance present in the outermost layer of the cell wall of Gram-negative bacteria and is known to play various roles in the interaction between pathogenic bacteria and eukaryotes. In particular, LPS is recognized as a foreign substance in the human body and activates the cellular defense system, thereby activating the inflammatory pathway, which is one of the immune defense systems.

When cells are stimulated by an external stimulator such as LPS, the expression of iNOS (inducible NO synthas) and COX-2 (cyclooxygenase-2) is induced, which in turn induces intracellular signal transduction substances that mediate the inflammatory response. Nitric oxide (NO) and prostaglandin E2 (PGE ₂ ) are released. Due to this excessive release of NO and PGE ₂ , an inflammatory reaction occurs in the human body and tissues.

In general, NO is a modulator that regulates normal physiological activities, such as controlling blood pressure, which is a physiological phenomenon, and acting as a neurotransmitter mediator, and plays a central role in the immune response as well. However, NO overexpressed by iNOS causes an inflammatory response and causes abnormalities in the immune system. In addition, COX-2 is _{an enzyme that converts arachidonic acid into prostaglandin (PGE 2} ). When COX-2 is overexpressed, PGE ₂ is overproduced and serves as another inflammatory pathway.

Non-steroidal anti-inflammatory drugs (NSAIDS), which are widely used agents for the treatment of most inflammatory diseases, are prostaglandins from arachidonicacid called cyclooxygenase (COX). By inhibiting the activity of enzymes involved in biosynthesis, it exhibits anti-inflammatory action, and since it causes serious side effects such as gastrointestinal disorders, liver disorders, and renal disorders in addition to the main therapeutic action, there are restrictions in long-term use (Rajakariar R et al. 2006).

On the other hand, melanin pigment produced in melanocytes of the skin is a phenolic polymer having a complex form of black pigment and protein, and plays an important role in inhibiting skin damage caused by ultraviolet rays irradiated from the sun. However, excessive synthesis and accumulation of melanin not only cause serious aesthetic problems such as freckles, freckles and senile surplus on the skin, but also promote skin aging and cause skin cancer. In particular, melasma is a disease in which light brown, dark brown, or even black hyperpigmented lesions occur on the central part of the face, such as the cheeks, forehead, nose, and chin, and occurs mainly in women, but rarely in men. Research has been conducted to discover markers that are specifically overexpressed in melasma and suppress them, and it has been reported that inhibiting the activity or expression of the tyrosinase enzyme can inhibit skin pigmentation.

Recently, the popularity of cosmetics using natural plant-derived resources that can minimize skin troubles is increasing, and research on natural cosmetic materials and products without side effects is increasing.

Republic of Korea Registration No. 10-1164083 (Announcement date: 2012.07.16.) Republic of Korea Registration No. 10-1964809 (Notice Date 2019.03.27.) Republic of Korea Registration No. 10-2125895 (Announcement date: 2020.06.23.)

As a result of continuous research on the NO activity inhibitory ingredients derived from natural plant ingredients developed and patented by the present inventors, the existing complex extracts do not reduce the NO activity inhibitory efficacy and prevent pigmentation of the skin through inhibition of tyrosinase activity The present invention was completed by knowing the optimal composition and composition ratio of the complex extract that can be done. That is, the present invention relates to a cosmetic comprising a complex extract using a herbal robert extract (and/or a fraction thereof), a tarragon leaf and stem (leaf/stem) extract, a safflower seed extract, and a reed extract, and cosmetics using the same, and a manufacturing method thereof would like to provide

The present invention for solving the above problems is to provide a cosmetic comprising a herbal extract, tarragon leaf and stem extract, safflower seed extract and reed extract.

In addition, the present invention is to provide a cosmetic comprising the cosmetic.

In addition, the present invention is to provide a method for preparing the cosmetic.

The cosmetic of the present invention has excellent anti-inflammatory effect by inhibiting NO production by LPS and inhibiting the intracellular activity of inflammatory markers iNOS, COX-2, TNFα, IL6 without cytotoxicity. Therefore, the cosmetic of the present invention is suitable for use as a cosmetic, and furthermore, it is possible to provide a natural cosmetic having an effect of inhibiting inflammation due to skin troubles, acne, etc. by applying it to cosmetics. In addition, the cosmetic of the present invention inhibits the activity of tyrosinase to prevent, inhibit or prevent pigmentation in the skin, thereby removing and preventing blemishes such as blemishes can be provided.

Hereinafter, the present invention will be described in more detail.

Herb robert is a kind of geranium (Geranium robertianum), and it is wild in Europe, and small reddish purple flowers bloom in summer.

Tarragon (Artemisia dracunculus, Tarragon) is a plant used as a spice because of its sweet aroma and spicy and bitter taste, which French consider the queen of spices. The leaves are mainly used for cooking. Tarragon is a tonic, good for the head, heart, and liver, and was considered a plant to improve stamina in the Middle Ages by helping to recover from fatigue. Tarragon stems grow about 50 to 90 cm tall, and the leaves are shiny and narrow, long, and green. Also, there is a characteristic that small flower buds are produced, but no fruits are produced because flowers do not bloom.

Safflower (Carthamus tinctorius L.), called safflower, is an annual or biennial herb belonging to the Asteraceae. Safflower is used as a dye, and has been used as a medicinal or cosmetic lip rouge, and has been used as a herbal medicine because it has effects such as blood flow, mouth blood, and pain relief. And, safflower seeds have been used medicinally as a blood circulation agent as the seeds of these safflowers.

Reed (Phragmites communis) is a herbaceous perennial plant belonging to the family Grapeaceae. Reed roots usually dig up to about 30-50 cm deep into the ground and are difficult to collect. According to the report, reed root contains a component called paracumaric acid and beta-cisosterol, which are known to help a lot in cerebrovascular diseases.

Comfrey (Symphytum officinale) is a dicotyledonous plant cultivated for medicinal or fodder purposes. In oriental medicine, the leaves and roots are used as a medicinal herb called Gamburi (甘富利). · It has been used as a medicine for sore throat and dermatitis.

Conventionally, the present applicant has developed and patented a cosmetic for a complex extract using herbal robert extract, tarragon leaf/stem extract and/or safflower seed extract. The activity inhibitory ability was very insufficient, and to increase the tyrosinase activity inhibitory effect, increasing a specific content of the extracts in the complex extract, or using other components, there was a problem that the NO activity inhibitory effect was inhibited. As a result of various studies and trials, the present invention was completed by developing a complex extract having an optimal composition and composition ratio having an excellent tyrosinase activity inhibitory effect without reducing the NO activity inhibitory effect.

The cosmetic of the present invention is Herb Robert as described above. As an invention using tarragon leaves and stems (hereinafter referred to as "leaf/stem"), safflower seeds and reeds, it is an invention using a complex extract including extracts of each of the natural plants.

A complex extract including the extract is included, and the complex extract contains the herb Robert extract and the tarragon leaf/stem extract in a weight ratio of 1: 0.5 to 1.5, preferably, the herb Robert extract and the tarragon leaf/stem extract in a weight ratio of 1: 0.5 to 1.0. can be included as If the amount of tarragon leaf/stem extract used is less than 0.5 weight ratio, the NO activity inhibition synergistic effect may be insignificant, and when used in excess of 1.5 weight ratio, the tyrosinase activity inhibitory effect is reduced, so it is recommended to mix and use within the above range.

In addition, the complex extract of the present invention contains safflower seed extract to increase the NO activity inhibitory effect and skin whitening effect while slightly reducing the toxicity of the herbal Robert extract, and the safflower seed extract to the herbal Robert extract is used in a weight ratio of 1: 0.8 to 2.0. As a result, preferably, it is good to include the safflower seed extract in a weight ratio of 1: 1.0 to 1.5 with respect to the herb Robert extract. At this time, if the amount of safflower seed extract used is less than 0.8 weight ratio, the amount used is too small, and the improvement effect due to its introduction may be insignificant. .

In addition, the complex extract of the present invention further comprises a reed extract and / or comfrey leaf extract for the tyrosinase activity inhibitory effect.

The amount of the reed extract used is 1: 0.5 to 1.5 weight ratio with respect to the herb Robert extract, preferably 1: 0.5 to 1.2 weight ratio, more preferably 1: 0.7 to 1.0 weight ratio, and in this case, it is recommended to use the reed extract If the amount used is less than 0.5 weight ratio, the tyrosinase activity inhibitory effect may be insufficient, and if it is used in excess of 1.5 weight ratio, it is an excessive use and there is no further increase in the tyrosinase activity inhibitory effect, but rather reduces the NO activity inhibitory effect, or rather skin cells It is recommended to use within the above range because it may cause skin trouble due to its toxicity.

In addition, the comfrey leaf extract serves to enhance the tyrosinase activity inhibitory effect while preventing skin trouble caused by the reed extract, and it is recommended to additionally use it together when the reed extract content is high. And, the amount thereof in the complex extract is 1: 0.1 to 0.5 weight ratio, preferably 1: 0.2 to 0.5 weight ratio, more preferably 1: 0.2 to 0.4 weight ratio with respect to the herb Robert extract. At this time, since the amount of comfrey leaf extract used is less than 0.1 weight ratio and the amount is too small, the effect due to the use of comfrey leaf extract may be insignificant. it's good

The herbal extract is prepared by mixing 15 to 35 parts by weight of dried herb Robert, preferably 20 to 30 parts by weight, more preferably 20 to 25 parts by weight based on 100 parts by weight of purified water to prepare a raw material; extracting the raw material by performing hot water extraction under 70 to 80° C. for 4 to 8 hours to obtain an extract; preparing a first filtrate obtained by first filtering the extract; After adding and stirring 1,2-hexanediol and ethylhexylglycerin to the first filtrate, secondary filtering to prepare an herbal robert extract may be used.

When preparing the herbal robert extract, the primary filtering may be performed with a filter membrane having an average pore size of 20 to 30 μm, preferably filtering with a filter membrane having an average pore size of 23 to 27 μm.

In addition, the secondary filtering may be performed with an average pore 8 to 15 μm filter membrane, preferably an 8 to 12 μm filter membrane.

In the present invention, the tarragon leaf/stem extract is prepared by mixing 10 to 30 parts by weight of dried tarragon leaves/stem, preferably 12 to 25 parts by weight, more preferably 12 to 18 parts by weight, based on 100 parts by weight of purified water. preparing; extracting the raw material by performing hot water extraction at 90 to 100° C. for 4 to 8 hours to obtain an extract; preparing a first filtrate obtained by first filtering the extract; After adding and stirring 1,2-hexanediol and ethylhexyl glycerin to the first filtrate, secondary filtering to prepare a tarragon leaf/stem extract may be used.

In the present invention, the safflower seed extract is obtained by extracting dried safflower seed powder with n-hexane under 15 to 30° C., followed by a first step of removing fat from the extract; A second step of obtaining an ethanol extract by extracting and filtering the fat-removed extract with an aqueous ethanol solution of 60 to 75% by volume, preferably at a concentration of 65 to 75% under 15 to 30° C.; Step 3 of concentrating and filtering the ethanol extract under reduced pressure to obtain a concentrate under reduced pressure; and 4 steps of filtering the concentrated under reduced pressure to obtain a safflower seed extract as a filtrate;

When preparing the safflower seed extract, step 1 is repeated 2 to 5 times, preferably 3 to 4 times to prepare an extract from which fat has been removed, and then the re-extraction process is performed with an aqueous ethanol solution in step 2. can

The concentration under reduced pressure of the three steps may be performed through a general method used in the art, and is performed to remove solvents and impurities.

And, the concentration under reduced pressure of the 3rd step and the filtering of the 4th step can be performed through a general reduced pressure concentration and filtering method used in the art, for example, the filtering is an average pore 8 ~ 15㎛ filter membrane, preferably can perform filtering with an 8 ~ 12㎛ filter membrane.

In the present invention, the reed extract is prepared by mixing 50 to 80 parts by weight of dried reed root, preferably 50 to 75 parts by weight, more preferably 55 to 70 parts by weight, based on 100 parts by weight of purified water; extracting the raw material by performing hot water extraction under 90 to 100° C. for 4 to 6 hours to obtain an extract; preparing a first filtrate obtained by first filtering the extract; After adding and stirring 1,2-hexanediol and ethylhexylglycerin to the first filtrate, a second filtering step to prepare an extract may be used.

The primary and/or secondary filtering may be performed in the same manner as the primary and secondary filtering used in preparing the herbal robert extract described above.

In the present invention, the comfrey extract is prepared by mixing 20 to 40 parts by weight of dried comfrey leaves, preferably 20 to 35 parts by weight, more preferably 20 to 30 parts by weight, based on 100 parts by weight of purified water; extracting the raw material by performing hot water extraction at 60 to 75° C. for 4 to 8 hours to obtain an extract; preparing a first filtrate obtained by first filtering the extract; After adding and stirring 1,2-hexanediol and ethylhexylglycerin to the first filtrate, a second filtering step to prepare an extract may be used.

The primary and/or secondary filtering may be performed in the same manner as the primary and secondary filtering used in preparing the herbal robert extract described above.

When the cosmetic of the present invention introducing the complex extract contains the complex extract at a concentration of 2.0% by weight or less, the RAW264.7 cell viability may be 96.00% or more, and preferably contains the complex extract in an amount of 0.125 to 2.0% by weight When doing this, RAW264.7 cell viability may be 96.5 to 102.0%, more preferably 97.5 to 102.0%.

In addition, in the cosmetic of the present invention, when the concentration of the complex extract is 0.1 to 1.0% by weight, when measured based on Equation 1 below, the inhibition rate of nitric oxide (NO) production in RAW264.7 cells is 20 to 45%, Preferably 22 to 43%, more preferably the NO production inhibition rate may be 25.0 to 40.0%.

[Equation 1]

NO production inhibition rate (%) = (A-B)/A × 100%

In Equation 1, A is the amount of NO production in RAW264.7 cells (100%) when the concentration of the complex extract in the cosmetic is 0%, and B is the amount of NO production in the RAW264.7 cells at a specific concentration of the complex extract in the cosmetic ( %) is the measured value.

The cosmetic of the present invention inhibits the activity of iNOS (induced NO synthas), COX-2 (cyclooxygenase-2), TNF-α (tumor necrosis factor α), and/or the interleukin receptor IL6, thereby NO activity It may have an inhibitory effect and/or an anti-inflammatory effect.

In addition, in the cosmetic of the present invention, when the concentration of the complex extract is 0.1 to 0.5% by weight, when measured based on Equation 2, the tyrosinase activity inhibition rate measured by Equation 2 below satisfies Equation 1 can

[Equation 2]

Tyrosinase activity inhibition rate (%) = (A-B) / A × 100%

In Equation 2, A is the absorbance at 480 nm of the control in which the concentration of the complex extract in the cosmetic is 0% by weight, and B is the absorbance at 480 nm of the cosmetic in which the concentration of the complex extract is 0.1 to 0.5% by weight,

[Equation 1]

12% ≤ (A-B) ≤ 30%, preferably 12.5% ≤ (A-B) ≤ 28.5%, more preferably 15.0% ≤ (A-B) ≤ 27.0%

In Equation 1, A is the tyrosinase activity inhibition rate of the cosmetic comprising the complex extract of the present invention, and B is the tyrosinase activity inhibition rate of the control group, where the control group is a herbal robert extract and tarragon leaf and stem extract 1 : Cosmetic containing complex extract included in a weight ratio of 2.2.

The cosmetic of the present invention uses a cosmetic comprising the above-described complex extract, and includes an aqueous solution; emulsion; the cosmetic; Spices; and purified water;

And, the cosmetic of the present invention, based on 100 parts by weight of the aqueous phase, 20 to 30 parts by weight of the emulsion, 1 to 30 parts by weight of a cosmetic excellent in inhibiting NO activity and tyrosinase activity, and 0.05 to 2 parts by weight of the fragrance Contains parts and the remainder of purified water, preferably, with respect to 100 parts by weight of the aqueous solution, 22 to 28% by weight of the emulsion, 5 to 30 parts by weight of the NO activity inhibitor, 0.1 to 1 parts by weight of the fragrance, and the remaining amount Contains purified water. At this time, if the emulsion is less than 20 parts by weight, there may be a problem in maintaining skin moisture due to insufficient oil content, and if it exceeds 35 parts by weight, excessive oil components are contained, which may cause problems such as obstruction of skin breathing or pimples on oily skin. . In addition, if the cosmetic content is less than 1 part by weight, the content is too small, so the effect of inhibiting NO activity and tyrosinase activity may not be seen, and if it exceeds 30 parts by weight, compatibility with other ingredients decreases due to excessive use The quality may be rather deteriorated, and the effect of inhibiting NO activity and tyrosinase activity due to excessive use is also almost insignificant, so it is uneconomical.

Among the cosmetic ingredients, general perfumes used in the art may be used, and specific examples thereof include Perfume 85147, Perfume 25399, and the like.

[Awards]

Among the cosmetic ingredients of the present invention, the aqueous solution includes a skin conditioning agent, a sequestering agent, a thickener, a moisturizing agent, a viscosity reducing agent, a sterilization preservative, and a solvent.

Among the components of the aqueous solution, the skin conditioning agent may be used alone or in a mixture of two or more selected from sodium hyaluronate, hyaluronic acid, licorice powder and hydrolite, preferably hyaluronic acid, Two or more selected from licorice powder and hydrolite, more preferably hyaluronic acid, licorice powder and hydrolite may be mixed and used. And, when using a mixture of hyaluronic acid, licorice powder and hydrolite, hyaluronic acid, licorice powder, and hydrolite are used in a weight ratio of 1: 0.01 to 0.2: 1-3, preferably hydrolite 1: 0.02 to 0.15: It is recommended to use the mixture in a weight ratio of 1.2 to 2.7.

Among the components of the aqueous solution, the sequestering agent serves to prevent deterioration by metal ions that may be contained in the formulation, and the amount used is 0.1 to 2 parts by weight, preferably based on 100 parts by weight of the skin conditioning agent. can be used 0.2 to 1.5 parts by weight, at this time, if the amount of the sequestering agent used is less than 0.1 parts by weight, the metal ion sequestration effect may fall, and if it is used in excess of 2 parts by weight, there is no effect of increasing the metal ion sequestration, so it is an uneconomic problem Because there may be, it is recommended to use within the above range. The sequestering agent may use a general one used in the art, preferably disodium EDTA, dipotassium EDTA, trisodium EDTA, tetrasodium EDTA A and rice phytate (rice phytate) can be used alone or a mixture of two or more selected from.

Next, among the components of the aqueous solution, the thickener serves to increase skin adhesion by imparting appropriate viscosity and stickiness to the formulation, and the amount of the thickener used is 0.5 to 2 parts by weight, preferably based on 100 parts by weight of the skin conditioning agent. 0.5 to 1.7 parts by weight can be used, and at this time, if the amount of xanthan gum used is less than 0.5 parts by weight, the amount used is too small and the effect of using it is insignificant. It is recommended to use within the above range because the stickiness may increase too much and there may be a problem of poor marketability. As the thickener, a general one used in the art may be used, and preferably, one selected from xantam gum, guar hydroxy trimonium chloride, and hydroxy ethyl cellulose may be used alone or in combination of two or more.

Next, among the components of the aqueous solution, the moisturizer serves to increase skin moisture along with the moisture evaporation blocking agent of the emulsion, and the amount used is 40 to 80 parts by weight, preferably 42 parts by weight, based on 100 parts by weight of the skin conditioning agent. ~ 70 parts by weight can be used, and at this time, if the amount of the moisturizer is less than 40 parts by weight, the skin moisturizing effect may fall, and even if used in excess of 80 parts by weight, there may be a non-economic problem because there is no moisturizing effect. good to use In addition, as a moisturizing agent, one type selected from methylpropanediol and glycerin may be used alone or two or more types may be mixed.

Next, the viscosity reducing agent among the components of the aqueous solution is used to maintain an appropriate viscosity to increase adhesion to the skin and skin absorption, and its amount is 45 to 90 parts by weight based on 100 parts by weight of the skin conditioning agent, Preferably, 50 to 85 parts by weight, more preferably 52 to 80 parts by weight, may be used. In this case, if the amount of the viscosity reducing agent used is less than 45 parts by weight or exceeds 90 parts by weight, the viscosity of the formulation is too high or low, There may be a problem in that the adhesive strength is too low or the stickiness is too increased to reduce the feeling of use. And, as the viscosity reducing agent, a general viscosity reducing agent used in the art may be used, preferably di(C2 to C5 alkylene) glycol, more preferably dipropylene glycol .

Next, among the components of the aqueous solution, the sterilization preservative is used to prevent contamination of cosmetics by microorganisms, and as long as it is a sterilization preservative that is not prohibited as a cosmetic material, it can be used without particular limitation, as a specific example, clofenesin etc. can be used. And, the amount thereof may be used in an amount of 2 to 10 parts by weight, preferably 4 to 8 parts by weight, based on 100 parts by weight of the skin conditioning agent, and if the amount used is less than 2 parts by weight, the effect of preventing contamination by microorganisms may be insufficient. There is, and using in excess of 10 parts by weight may cause skin troubles as excessive use, so it is recommended to use within the above range.

Next, as the solvent in the aqueous phase component, one type selected from hexanediol, propylene glycol, butylene glycol, and pentylene glycol may be used alone or a mixture of two or more types may be used, preferably hexanediol and Butylene glycol may be mixed and used, or more preferably, 1,2-hexanediol and 1,3-butylene glycol may be mixed and used. In addition, the amount of solvent used is 30 to 60 parts by weight, preferably 35 to 50 parts by weight, based on 100 parts by weight of the skin conditioning agent. and it is uneconomical to use in excess of 60 parts by weight.

[Emulsion]

Among the cosmetic ingredients of the present invention, the emulsion includes a moisture evaporation blocking agent, tocopheryl acetate, a skin conditioning agent, an emulsifier, a skin softener, a nonionic surfactant, and a film former.

Among the components of the emulsion, the moisture evaporation blocker serves to maintain proper moisture during the manufacture of cosmetics and to maintain an appropriate moisture content together with the moisturizer when the prepared formulation is applied to the skin. Preferably, one or two selected from caprylic / capric triglyceride oil, MCT oil, and squalane may be mixed and used, more preferably MCT oil and squalane 5 to 10: It is recommended to use it by mixing it in 1 weight ratio.

Next, among the components of the emulsion, the tocopheryl acetate serves to prevent the formulation from being oxidized and deteriorated over time, and the amount used is 0.1 to 5 parts by weight, preferably based on 100 parts by weight of the moisture evaporation barrier. 0.4 to 2 parts by weight may be used. At this time, if the amount of tocopheryl acetate used is less than 0.1 parts by weight, the antioxidant effect may not be seen because the amount used is too small, and if the content exceeds 5 parts by weight, there may be a problem of causing irritation to the skin. good to use

Next, among the emulsion components, the skin conditioning agent serves to supply nutrients to the skin, and a single type or a mixture of two types selected from shea butter, coconut oil, macadamia nut oil, and jojoba oil can be used. , Preferably, shea fat and coconut oil can be mixed and used in a weight ratio of 1:0.4 to 0.8. The amount of the skin conditioning agent to be used is 10 to 35 parts by weight, preferably 13 to 30 parts by weight, based on 100 parts by weight of the moisture evaporation blocking agent. There may be a problem that may be insignificant, and if the content exceeds 35 parts by weight, the amount used is too excessive and there may be a problem of remaining on the skin surface after absorption of a certain part of the skin, so it is recommended to use it within the above range.

Next, among the components of the emulsion, the emulsifier is for improving compatibility and emulsifying power between the emulsion compositions, and general ones used in the art may be used, preferably cetearyl glucoside-cetearyl alcohol (cetearyl glucoside). and cetearyl alcohol), cetearyl alcohol, glyceryl stearate, PEG-6 sorbitan stearate, PEG-20 stearate, PEG-40 stearate, and PEG-100 stearate alone or Two or more kinds may be mixed and used, and more preferably, a species selected from cetearyl glucoside-cetearyl alcohol and cetearyl alcohol may be used alone or two or more types may be mixed. In addition, the amount of the emulsifier used is 30 to 60 parts by weight, preferably 40 to 55 parts by weight, based on 100 parts by weight of the skin conditioning agent. It may not work well, and it is uneconomical to use in excess of 60 parts by weight.

Next, among the components of the emulsion, the skin softening agent serves to soften rough skin, and the amount used is 2 to 10 parts by weight, preferably 3 to 7.5 parts by weight, based on 100 parts by weight of the skin conditioning agent. At this time, if the amount of the skin softener used is less than 2 parts by weight, there may be a problem that the effect may be insignificant. It is recommended to use within the above range. And, the skin emollient is one or more selected from an oil agent containing one or more medium chain monoglycerides, glyceryl caprate, polyglyceryl-2-laurate, and polyglyceryl-10-laurate. It may be used in combination, and preferably, one or more selected from an oil agent containing one or more medium chain monoglycerides and polyglyceryl-10-laurate may be used alone or in combination of two or more. In addition, a specific example of the emulsifier containing one or more medium-chain monoglycerides includes AKOLINE MCM and the like.

Next, among the components of the emulsion, the nonionic surfactant serves as a dissolution aid for the compositions together with the emulsifier, and general nonionic surfactants used in the art may be used, and specific examples include Tween 20, Tween 40 and Tween 60 may be used alone or in combination of two or more. And, the amount of the nonionic surfactant may be used in an amount of 7 to 20 parts by weight, preferably 10 to 15 parts by weight, based on 100 parts by weight of the skin conditioning agent.

Next, among the emulsion components, the film forming agent serves to protect the skin from unnecessary external contaminants after the formulation is applied to the skin, and general ones used in the art may be used, and as a specific example, cepi Plus 400 (sepiplus 400) or the like can be used. In addition, the amount of the film-forming agent used is 1.5 to 8 parts by weight, preferably 2.5 to 7 parts by weight, of the film-forming agent based on 100 parts by weight of the skin conditioning agent, and if the amount used is less than 1.5 parts by weight, the amount used is too much. Since it is small, the effect of using it cannot be seen, and if it is used in excess of 8 parts by weight, solid content in the emulsion component may occur, so it is recommended to use it within the above range.

In addition, the emulsion may further include a thickening stabilizer. The amount used is 1 to 10 parts by weight, preferably 2 to 8 parts by weight, based on 100 parts by weight of the skin conditioning agent. It is recommended to use within the above range because there may be problems. In addition, as the thickening stabilizer, one or two selected from sodium polyacrylate, polyacrylate, polyisobutene, carbomer, polysorbate 20, polysorbate 50, polysorbate 60 and polysorbate 80 A mixture of the above may be used, and preferably, one type selected from polyacrylate, polyisobutene, and polysorbate 80 may be used alone or two or more types may be mixed.

Hereinafter, a method for manufacturing the cosmetic of the present invention described above will be described.

The cosmetic of the present invention is prepared by preparing an oil phase and an aqueous phase, respectively, and then emulsifying them under a specific temperature and condition, the first step of preparing the aqueous phase and the aqueous phase, respectively; a second step of preparing a mixture by adding an aqueous solution to the emulsion and then stirring; It can be prepared by performing a process comprising; mixing the mixture of two steps, the cosmetic, fragrance and purified water containing the complex extract of the present invention under 40 to 50 ° C. and stirring at a stirring speed of 2,000 to 3,000 rpm; have.

And, the emulsion of the first step is mixed with a skin conditioning agent, tocopheryl acetate, an emulsifier, a skin softener, a moisture evaporation blocking agent and a nonionic surfactant, and then at a temperature of 60 to 75 ℃, preferably 65 to 72 ℃ A solution may be prepared by heating and stirring so as to become

In addition, the aqueous solution of step 1 can be prepared by mixing a skin conditioning agent, a sequestering agent, a thickener, a moisturizing agent, a viscosity reducing agent, a sterilization preservative, and a solvent, and then heating and stirring to 50 ~ 60 ℃.

Components used in the emulsion and aqueous phase and their usage are the same as described above.

In step 2, a mixture can be prepared by adding an aqueous solution to the emulsion and then emulsifying for 1 to 4 minutes at a stirring speed of 2,000 to 3,000 rpm under 70 to 80 ° C.

And, the cosmetic in step 3 may be prepared by the method described above.

The cosmetic of the present invention thus prepared may have a pH of 5.4 to 6.2, preferably a pH of 5.8 to 6.0, and more preferably a pH of 5.85 to 5.95.

The cosmetics of the present invention described above can manufacture various types of cosmetics, for example, cosmetics such as cream type and serum type.

Hereinafter, the present invention will be described in more detail based on examples. However, it should not be construed to limit the scope of the present invention by the following examples.

[Example]

Preparation Example 1: Preparation of Herb Robert Extract

Raw materials were prepared by mixing 23 parts by weight of dried herb Robert with respect to 100 parts by weight of purified water.

Next, the raw material was extracted with hot water at about 78 to 80° C. for 6 hours to obtain an extract.

Next, the extract was filtered with a filter membrane having an average pore size of 25 μm to obtain a primary filtrate.

Next, 5 ml of 1,2-hexanediol and 0.1 ml of ethylhexyl glycerin were added to 94.9 ml of the filtrate filtrate, and after sufficient stirring, the stirred solution was subjected to secondary filtering with a filter membrane having an average pore size of 10 μm. Herb Robert extract was prepared.

Preparation Example 2: Preparation of tarragon leaf/stem extract

A raw material was prepared by mixing 16 parts by weight of dried tarragon leaves and stems with respect to 100 parts by weight of purified water.

Next, the raw material was extracted with hot water at 95 ~ 98 ℃ for 6 hours to obtain an extract.

Next, the extract was filtered with a filter membrane having an average pore size of 25 μm to obtain a primary filtrate.

Next, 5 ml of 1,2-hexanediol and 0.1 ml of ethylhexyl glycerin were added to 94.9 ml of the filtrate filtrate, and after sufficient stirring, the stirred solution was subjected to secondary filtering with a filter membrane having an average pore size of 10 μm. Tarragon leaf/stem extracts were prepared.

Preparation Example 3: Preparation of Safflower Seed Extract

The dried safflower seeds were pulverized to prepare safflower seed powder. Next, the safflower seed powder and n-hexane having a concentration of 99.99% by volume as a non-polar solvent were mixed in a weight ratio of 1:22, followed by extraction at 24 to 25° C., and then fat was removed. The extraction and fat removal were repeated three times to obtain an extract from which fat was removed. Next, the extract from which the fat ratio has been removed and an aqueous ethanol solution having a concentration of 72 to 73% by volume were mixed in a 1:10 weight ratio, followed by extraction at 24 to 25° C. to obtain an ethanol extract. Next, the ethanol extract was concentrated under reduced pressure, and then filtered to obtain a concentrate under reduced pressure. Next, the ethanol extract was concentrated under reduced pressure, and then filtered to obtain a concentrate under reduced pressure. Next, the safflower seed extract was prepared by filtering the reduced pressure concentrate with a filter membrane having an average pore size of 10 μm.

Preparation Example 4: Preparation of reed extract

A raw material was prepared by mixing 63 parts by weight of dried reed root with respect to 100 parts by weight of purified water.

Next, the raw material was extracted with hot water at about 85 to 90° C. for 5 hours to obtain an extract.

Next, the extract was filtered with a filter membrane having an average pore size of 25 μm to obtain a primary filtrate.

Next, 5 ml of 1,2-hexanediol and 0.1 ml of ethylhexyl glycerin were added to the filtrate, and after sufficient stirring, the stirred solution was subjected to secondary filtering with a filter membrane having an average pore size of 10 μm to prepare a reed extract did

Preparation Example 5: Preparation of Comfrey Leaf Extract

A raw material was prepared by mixing 25 parts by weight of dried comfrey leaves with respect to 100 parts by weight of purified water.

Next, the raw material was extracted with hot water at about 70 to 72° C. for 5 hours to obtain an extract.

Next, the extract was filtered with a filter membrane having an average pore size of 25 μm to obtain a primary filtrate.

Next, 5 ml of 1,2-hexanediol and 0.1 ml of ethylhexylglycerin were added to the filtrate, and after sufficient stirring, the stirred solution was subjected to secondary filtering with a filter membrane having an average pore size of 10 μm to obtain a comfrey extract. prepared.

Example 1: Preparation of cosmetics (complex extract)

The herbal robert extract of Preparation Example 1, the tarragon leaf/stem extract of Preparation Example 2, the safflower seed extract of Preparation Example 3 and the reed extract of Preparation Example 4 were mixed in a weight ratio of 1: 0.75: 1.0: 0.80 to prepare a complex extract as a cosmetic. did

Examples 2 to 3

Examples 2 to 3 were carried out by using the same extract as in Example 1, but by preparing a complex extract by varying the weight ratio of each extract as shown in Table 1 below.

Example 4

The same extract as in Example 1 was used, but the comfrey leaf extract was additionally used in a weight ratio of 0.3 to prepare a complex extract as a cosmetic.

Comparative Example 1

Herb Robert extract of Preparation Example 1 alone was prepared as a NO activity inhibitor.

Comparative Example 2

Tarragon leaf/stem extract alone of Preparation Example 2 was prepared as a NO activity inhibitor.

Comparative Example 3 ~ Comparative Example 6

A complex extract (NO activity inhibitor) was prepared in the same manner as in Example 1, but each complex extract having the composition shown in Table 1 was prepared as an NO activity inhibitor.

division
(weight ratio) Herb Robert
extract Tarragon leaves/stalks
extract safflower seed
extract Reed
extract comfrey leaf
extract Example 1 One 0.75 1.0 0.8 - Example 2 One 0.75 1.0 0.6 - Example 3 One 0.75 1.0 1.2 - Example 4 One 0.75 1.0 0.8 0.30 Comparative Example 1 One - - - - Comparative Example 2 - One - - - Comparative Example 3 One 2.2 - - - Comparative Example 4 One 1.8 1.0 0.8 - Comparative Example 5 One 0.75 1.0 0.2 - Comparative Example 6 One 0.75 1.0 1.65 -

Experimental Example 1: Measurement of cell viability for complex extract (NO activity inhibitor)

In order to confirm the cytotoxicity of the extract, RAW 264.7 cells, a mouse macrophage cell line, were measured as an inflammation model.

(1) cell culture

RAW 264.7 cells, a mouse macrophage cell line, were prepared in DMEM medium (high glucose, Using Thermo's SH30243.01), it was cultured in _{a 5% CO 2 incubator using a 100 mm cell culture dish.} Cells that reached confluence were maintained by subculture using a scraper (SPL, 90030).

(2) Cell viability experiment

Dehydrogenase present in mitochondria in living cells produces a chromogenic substance called formazan from WST (Tetrazolium Salt). Therefore, it is possible to measure the number of living cells by measuring it. In a 96-well cell culture plate (Corning 3595), ^{RAW 264.7 cells at a concentration of 1×10 4} cells/well were treated with DMEM culture medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/stereptomycin. Incubate for 24 hours. Thereafter, the sample is replaced with a DMEM medium containing each concentration of the complex extract (0%, 0.125% by weight, 0.25% by weight, 0.5% by weight, 1% by weight, 2% by weight, 4% by weight) and cultured for 48 hours. Cell viability was measured for each concentration based on Equation 2 below by using EZ-CYTOX reagent (EZ-1000 from Daeilab) and measuring the absorbance at 450 nm for the cells that were cultured, and the results are shown in Table 2 below. . The measurement results in Table 1 below show the average value of three repeated experiments, and are relative values measured based on the control (0 wt% of the extract).

[Equation 2]

Cell viability = (absorbance in the sample added group / absorbance in the untreated group) × 100

division 0 wt% 0.125 wt% 0.25% by weight 0.5% by weight 1% by weight 2% by weight control 100% - - - - - Comparative Example 1 - 100.03 100.26 101.17 102.68 103.81 Comparative Example 2 - 99.98 98.06 97.02 94.32 91.22 Comparative Example 3 - 100.47 100.52 100.85 102.57 100.66 Comparative Example 6 - 98.23 97.02 95.33 90.35 84.98 Example 1 - 100.05 100.46 100.97 101.28 101.03 Example 2 - 100.12 100.61 101.10 102.88 101.57 Example 3 - 99.88 100.20 100.58 100.97 100.45 Example 4 - 100.02 99.84 99.35 98.92 98.09

Looking at the experimental results in Table 2, the test group treated with the herbal robert extract of Comparative Example 1 at a concentration of 2.0% or less did not show a statistically significant difference in cell viability compared to the control group, so RAW264 at a concentration of 2.0% or less .7 showed no cytotoxicity to cells. And, in the case of Comparative Example 2 in which the tarragon leaf/stem extract was used alone, compared with the control group and Comparative Example 1, the tarragon leaf/stem extract was somewhat toxic, resulting in a relatively low cell viability.

In addition, in the case of Comparative Example 6 in which the reed extract was used in excess of 1.5 weight ratio, when compared with Example 3, the cytotoxicity to RAW264.7 cells was high.

On the other hand, in the case of Examples 1 to 4, the overall cell viability was very good. And, comparing Example 1, Example 3, and Comparative Example 6, when the amount of the reed extract used was high, there was a tendency that the cell viability is low. However, it was confirmed that when the comfrey leaf extract was used (Example 4), it was possible to prevent a decrease in cell viability due to an increase in the tarragon leaf/stem and reed extract content.

Experimental Example 2: Measurement of NO production inhibition of complex extract

(1) Treatment of test substances

RAW 264.7 cells were dispensed in a 60 mm plate at 2×10 ⁶ cells/dish density and adhered to the plate for 24 hours. In order to induce an inflammatory response, a fresh medium containing LPS (lipopolysaccharide) at a concentration of 1 μg/ml and a test substance was treated and cultured for 24 hours.

(2) Nitric oxide (NO) production measurement experiment

The level of NO produced in RAW 264.7 cells and present in the culture medium was measured using a NO detection kit based on a Griess reaction (intron's 21021). After aliquoting 100 μl of the culture solution in which NO is presumed to be present in 96 well-plates, 50 μl of N1 buffer (sulfanilamide) is added and reacted at room temperature for 10 minutes. Then, 50 μl of N2 buffer (naphthylethylenediamine) was added, and after reacting at room temperature for 10 minutes, absorbance was measured at 540 nm, and each NO production amount was calculated using a standard calibration curve obtained using a nitrite standard. As a positive control, 50 μM of L-NMMA (N ^G -Methyl-L-arginine acetate salt, M7033 from Sigma) was used.

Each result confirms the statistical significance of the test result using the t-test (paired t-test, two-sided verification) function of Microsoft's Office Excel 2007, and the results are shown in Table 3 below. And, the NO production inhibition rate of Table 3 was calculated based on the following Equation 1.

In addition, the concentration of the complex extract was adjusted by dilution with purified water.

[Equation 1]

NO production inhibition rate (%) = (A-B)/A × 100%

In Equation 1, A is the amount of NO production in RAW264.7 cells (100%) when the concentration of the complex extract in the cosmetic is 0%, and B is the amount of NO production in the RAW264.7 cells at a specific concentration of the complex extract in the cosmetic ( %) is the measured value. The measurement results in Table 2 below show the average value of three repeated experiments, and are relative values measured based on the control (0 wt% of the extract).

NO production inhibition rate (%) 0 wt% 0.125 wt% 0.25% by weight 0.5% by weight 1% by weight 2% by weight control 0% - - - - - Positive control (L-NMMA 50 μM) 61.05% - - - - - Comparative Example 1 - 16.62% 19.98% 22.07% 24.76% 22.54% Comparative Example 2 - 1.24% 2.35% 3.88% 3.96% 3.98% Comparative Example 3 - 25.34% 26.52% 31.97% 33.25% 31.17% Comparative Example 4 - 26.23% 28.55% 32.31% 35.19% 32.78% Comparative Example 5 - 25.10% 26.03% 30.92% 33.18% 30.46% Comparative Example 6 - 22.07% 23.14% 25.38% 27.32% 22.39% Example 1 - 25.43% 26.72% 31.81% 34.13% 32.38% Example 2 - 25.22% 26.08% 31.52% 33.88% 31.81% Example 3 - 24.25% 25.37% 29.34% 30.21% 28.34% Example 4 - 27.31% 32.21% 37.30% 38.71% 33.13%

Looking at the measurement results in Table 3, the positive control L-NMMA inhibited the NO production of RAW264.7 cells by about 61% in the 50 μM concentration range. In addition, it can be confirmed that the herb Robert extract of Comparative Example 1 inhibits the production of nitirc oxide (NO) in RAW264.7 cells at a statistically significant level in the concentration range of 0.125 to 2.0%. It was confirmed that about 10 to 25% of NO production was inhibited when treated with 0.125 wt% to 1 wt%, and at 2 wt%, there was a problem in that the NO production inhibition rate was rather reduced. And, in the case of the tarragon leaf / stem extract of Comparative Example 2, the NO production inhibitory rate effect was insignificant.

On the other hand, in Comparative Example 3 using a mixture of herb Robert extract and tarragon leaf/stem extract, compared to Comparative Example 1, overall excellent NO production inhibition rate, and a high NO production inhibition rate even at 0.5 to 1% by weight.

In addition, as compared with Comparative Example 3 using a mixture of herb Robert extract and tarragon leaf/stem extract, in the case of Examples 1 to 4, which are complex extracts further introduced with safflower extract, reed extract and/or comfrey leaf extract, Comparative Example 3 It showed a similar to, equivalent to, or somewhat better inhibition of NO production.

However, in the case of Comparative Example 6 in which the reed extract was used in excess of 1.5 weight ratio, there was a problem in that the NO production inhibition rate effect was rather significantly reduced compared to Example 3 (1.2 weight ratio).

Experimental Example 3: Measurement of inhibition of tyrosinase activity of the complex extract

Inhibition of tyrosinase activity is generally measured by a spectroscopic method. In this experiment, it was carried out by the method of Vanni et al. (Vanni A. at al., Annali di Chimica, 80(35), 1990). Tyrosinase is an enzyme purified from potatoes or mushrooms, and in this experiment, those extracted from mushrooms were used. After mixing 1.0 ml of 0.1M potassium phosphate buffer (pH 6.8), 1.0 ml of 0.3 mg/ml L-tyrosine aqueous solution, and 0.1 ml of 1250 unit/ml mushroom tyrosinase (Sigma-Aldrich, USA) Here, 0.2 ml of each sample solution was added according to the concentration, followed by enzymatic reaction at 37° C. for 10 minutes. Then, the absorbance of the reaction solution was measured at 480 nm (JASCO V-550, japan) and calculated according to Equation 2 below to obtain a rate of inhibition of tyrosinase enzyme activity at a concentration of 0.5 wt % of the complex extract. At this time, the concentration of the complex extract was adjusted by diluting it with purified water.

As a positive control group, a 0.5 wt% concentration of arbutin at the same concentration as the experimental group was used, and as a control group for the experimental group, a reaction solution without addition of the complex extract was used. The results are shown in Table 4 below.

[Equation 2]

Tyrosinase activity inhibition rate (%) = (A-B) / A × 100%

In Equation 2, A is the absorbance at 480 nm of the control having a complex extract concentration of 0%, and B is the absorbance at 480 nm of the cosmetic having a complex extract concentration of 0.50 wt%.

division Inhibition rate (%) of tyrosinase activity at a concentration of 0.50 wt% of the complex extract Control (0 wt%) - arbutin 34.12% Comparative Example 3 11.61% Comparative Example 4 20.32% Comparative Example 5 15.70% Comparative Example 6 33.41% Example 1 29.67% Example 2 27.45% Example 3 33.30% Example 4 34.37%

Looking at the experimental results in Table 4, compared to Comparative Example 3, which is a complex extract prepared only with herb Robert and tarragon leaf/stem extract, it was confirmed that Examples 1 to 4 had a tyrosinase activity inhibition rate of 12% or more overall. .

And, in the case of Comparative Example 4, in which the tarragon leaf/stem extract was used in excess of 1.5 weight ratio, there was a problem in that the tyrosinase activity inhibition rate was rather sharply decreased compared to that of Example 1.

In addition, in the case of Comparative Example 5 using the reed extract in a 0.2 weight ratio that is less than 0.5 weight ratio, there was a problem that the tyrosinase activity inhibitory effect was very poor, compared to Examples 1 and 2, and the reed extract exceeded 1.5 weight ratio. In the case of Comparative Example 6 used in a weight ratio of 1.65, when compared with Example 3, there was no longer an effect of increasing inhibition of tyrosinase activity, and as described above, there was only a problem in that only the inhibitory effect of NO production was rather inhibited.

Preparation Example 1: Cosmetic Preparation

(1) Preparation of aqueous solution

After the components of Table 5 were completely dispersed in the solvent, purified water was added, and then the aqueous solution was prepared by heating and stirring to 56 ~ 57 ℃ and completely dissolving.

award division Kinds parts by weight skin conditioning Hyaluronic acid (1% concentration) 39.2 Licorice Powder 2 Hydrolite-5 58.8 sequestering agent EDTA-2NA 0.39 thickener Xanthan Gum (Keltrol F) 0.98 moisturizer glycerin 49.06 viscosity reducing agent dipropylene glycol 58.83 solvent 1,2-Hexanediol 19.61 1,3-butylene glycol 19.61 sterilization preservative Clofenesin 5.88

(2) Preparation of emulsion

After mixing the components in Table 5 below, mixing and dissolving while stirring with an azimuth mixer at 68° C., a film forming agent was added, and uniformly dispersed and mixed to prepare an emulsion.

emulsion division Kinds Usage (parts by weight) skin evaporator MCT Oil 87.5 squalane 12.5 tocopheryl acetate Vit-E Acetate 0.625 skin conditioning shea butter 12.50 coconut oil 6.25 emulsifier cetearyl glucoside and
Cetearyl Alcohol (Emulgade PL 68/50) 18.75 Cetearyl Alcohol (KALCOL 6850) 25 skin emollient one or more medium chain monoglycerides
Included fee (AKOLINE MCM) 5 nonionic surfactant Tween 60 12.5 film forming agent SEPIPLUS 400 3.75

(3) Put 100 parts by weight of the previously prepared aqueous phase solution into the main emulsification tank, put 25 parts by weight of the oil phase in a separate oil phase tank, and heat each to 80° C. made it Next, after adding the emulsion, it was emulsified for about 3 minutes at a speed of 2,500 rpm with a Homer mixer and 40 rpm with an agitator. After the emulsification is finished, the contents are cooled by using an agitator at a speed of 12 rpm, and then 22 parts by weight of the complex extract of Example 1 and 0.12 parts by weight of a fragrance (Perfume 85147) are added to the contents at 45° C. A creamy soft cream type cosmetic (milky white cream) was prepared.

Preparation Example 2: Cosmetic Preparation

A soft cream type cosmetic (milky white cream) was prepared in the same manner as in Preparation Example 1, except that 27 parts by weight of the complex extract of Example 2 was used instead of 22 parts by weight of the complex extract of Example 1 to prepare cosmetics.

Through the above Examples and Experimental Examples, the complex extract (cosmetics) of the present invention containing a specific extract in an optimal composition and composition ratio has an excellent anti-inflammatory effect, has an oxidative stress defense effect, and induces skin pigmentation such as spots It was confirmed that there is an effect of inhibiting the tyrosinase activity. As such, the present invention can provide functional cosmetics of various formulations using cosmetics.

Claims (9)

delete delete Herb Robert extract, tarragon leaf and stem extract, safflower seed extract, reed extract and comfrey leaf extract 1: 0.5 ~ 1.0: 1.0 ~ 2.0: 0.7 ~ 1.5: Contains a complex extract comprising 0.2 ~ 0.4 weight ratio,
When the concentration of the complex extract is 0.1 to 1.0% by weight, when measured based on Equation 1 below, the inhibition rate of nitric oxide (NO) production in RAW264.7 cells is 20 to 45%,
When the concentration of the complex extract is 0.1 to 0.5% by weight, when measured based on Equation 2, the inhibition of tyrosinase activity measured by Equation 2 below satisfies Equation 1 below. and cosmetics having the ability to inhibit tyrosinase activity;
[Equation 1]
NO production inhibition rate (%) = (AB)/A × 100%
In Equation 1, A is the amount of NO production (100%) in RAW264.7 cells when the concentration of the complex extract in the cosmetic is 0%, and B is the amount of NO production in the RAW264.7 cells at a specific concentration of the complex extract in the cosmetic. (%) is the measured value,
[Equation 2]
Tyrosinase activity inhibition rate (%) = (AB) / A × 100%
In Equation 2, A is the absorbance at 480 nm of the control in which the concentration of the complex extract in the cosmetic is 0% by weight, and B is the absorbance at 480 nm of the cosmetic in which the concentration of the complex extract is 0.1 to 0.5% by weight,
[Equation 1]
12% ≤ (AB) ≤ 30%
In Equation 1, A is the tyrosinase activity inhibition rate of the complex extract, and B is the tyrosinase activity inhibition rate of the control group, wherein the control is a combination containing herb Robert extract and tarragon leaf and stem extract in a weight ratio of 1: 2.2 It is an extract. [4] The cosmetic according to claim 3, wherein when the complex extract is contained in an amount of 2 wt% or less, the RAW264.7 cell viability is 96.00% or more. delete delete aqueous solution; emulsion; The cosmetic of claim 3 or 4; Spices; and purified water;
The aqueous solution includes a skin conditioning agent, a sequestering agent, a thickener, a moisturizing agent, a viscosity reducing agent, a sterilization preservative and a solvent,
The emulsion is a cosmetic, characterized in that it comprises a moisture evaporation blocking agent, tocopheryl acetate, a skin conditioning agent, an emulsifier, a skin softener, a nonionic surfactant and a film former. delete Step 1 of preparing an aqueous phase and an emulsion, respectively;
Step 2 of preparing a mixture by adding an aqueous solution to the emulsion and then stirring;
3 steps of mixing the mixture, complex extract, flavoring and purified water at 40-50 ° C. and stirring at a stirring speed of 2,000-3,000 rpm; performing a process comprising;
The complex extract comprises herb Robert extract, tarragon leaf and stem extract, safflower seed extract, reed extract and comfrey leaf extract in a weight ratio of 1: 0.5 to 1.0: 1.0 to 2.0: 0.7 to 1.5: 0.2 to 0.4 by weight. A method for manufacturing a revitalizing cosmetic having NO activity inhibitory ability and tyrosinase activity inhibitory activity.