KR102162904B1 - Hair coloring composition and the manufacturing process having antimicrobial and antiflammatory and deodorizing activity and ammonia odor removal effet - Google Patents

Abstract

The present invention relates to a hair colorant composition having effects on antibacterial, skin irritation alleviation, deodorization, and ammonia odor removal, wherein the hair colorant composition contains lavender water and lavender oil as active components, and further contains a lemon balm leaf extract and a lemon grass extract. The hair colorant composition according to the present invention has excellent effects on antibacterial, skin irritation alleviation, deodorization, and ammonia odor removal, is harmless to the human body due to less skin irritation, is easy to be applied to hair products such as hair colorants, and is a differentiated hair colorant product with excellent antibacterial and skin irritation relieving functions, and reducing preservatives and irritation alleviating agents to have excellent economic feasibility and low manufacturing cost compared to competitive hair colorant products.

Description

Hair coloring composition and the manufacturing process having antimicrobial and antiflammatory and deodorizing activity and ammonia odor removal effet}, antibacterial, relieving skin irritation, deodorizing and removing ammonia odor

The present invention relates to a hair-dye composition, and in particular, to a hair-dye composition that maximizes antibacterial and deodorant effects using lavender water and lavender oil as raw materials, and has an effect on antibacterial, alleviating skin irritation, deodorizing and removing ammonia odor.

The principle of general dyeing in hair dye products is that the essential components of the oxidizing dye intermediate, oxidizing agent, and alkali cause oxidative polymerization in the cortex layer inside the hair, causing discoloration and color development at the same time to complete the dyeing.

The dyeing principle of the plant-metal mixed dye is that if the first agent, pyrogallol, is applied to the hair first, it penetrates and softens the hair. After 20 minutes, when the second agent, iron sulfate, is applied over, it forms a complex salt with pyrogallol in the hair and turns black. It is dyed while doing it.

The principle of acid dye is that the dye does not penetrate inside the hair and heat is applied to coat the dye on the surface of the hair.

In general, hair dyeing agents can be classified into permanent hair dyeing agents, semi-permanent hair dyeing agents, and temporary hair dyeing agents depending on how long the color lasts and the difference in dyeing effect.

Permanent hair dyes allow pigment-forming substances to penetrate into the Cortex or Medula layer inside the hair, causing chemical changes, so that the effect of dyeing lasts for a long time.

Permanent hair dye is composed of two agents: agent 1 and agent 2, and agent 1 contains dyes and alkalis such as PPD components, and agent 2 contains components that act as developer and oxidizing agents such as hydrogen peroxide, so that the melanin of hair It decomposes and decolorizes the pigment, and at the same time, it changes color by penetrating the artificial pigment into the hair.

When dyeing, the first agent and the second agent are mixed and applied to the hair. At this time, the oxidation reaction occurs due to the mixing of the main ingredients, and the resulting decolorization and color development are not easily removed by shampoo or cleanser.

Semi-permanent hair dyes are coated colors or hair manicures that do not have side effects or damage to the hair, and are dyed only on the surface of the hair, so the color lasting is short.

Semi-permanent hair dyes are suitable for dyeing bleached hair, and are especially effective in giving highlights to specific areas of hair after partial bleaching. The dyeing period lasts 3-4 weeks, but the color gradually disappears as the shampoo is repeated, so it changes to various colors. If you want, you can use a semi-permanent hair dye.

Temporary hair dyes are different from permanent dyes that cannot be changed once, but after dyeing, only one shampoo will remove all dyed colors, such as hair mascara, color spray, color mousse, and hair spray.

Because the particle size is large, it cannot penetrate the epidermal layer, so it is easily cleaned.

The color of the prescription is mainly composed of black iron oxide and dye, and oxide dyes are also used.

Among these, permanent hair dyes can be divided into vegetable, metallic, and oxidized hair dyes.

Plant-based hair dyes are made of henna, indigo leaf powder, tenuscacia leaf powder, and senna leaf powder, which have low dyeing effect and are difficult to dye brightly.

Metal hair dyes use the principle that changes when lead is oxidized, and are toxic to humans, so they are not used.

Oxidative hair dye has good dyeing effect and can be dyed in bright colors and is most widely used.

The first agent contains a diamine compound and an alkali agent, which is a dye intermediate, and the second agent is composed of an oxidizing agent. The dye intermediate of the first agent is a color source material that changes to a dye when mixed with an oxidizing agent to give a color. As a dye intermediate, p-phenylenediamine + p-aminophenol (dark brown-red purple), o-aminophenol (yellow brown), p aminophenol (dark brown), mononitro-p-phenylenediamine (red), p-toluenediamine Different colors can be made according to the ratio, such as (dark brown-black brown) and p-phenylenediamine (black).

In addition, the alkali agent of the first agent swells and softens the hair and helps the dye to penetrate into the hair.These alkali agents include ammonia or monoethanolamine.Ammonia has a strong odor and irritation, although the dyeing lasts a long time, and monoethanolamine is There is no odor and there is no irritation to the scalp, but it remains in the hair because it is non-volatile, and the damage is severe and it is difficult to dye because it penetrates the hair slowly.

The second agent, the oxidizing agent, decolorizes melanin, the natural pigment of the hair, and acts on the dye intermediate to become a dye. This oxidizing agent refers to hydrogen peroxide.

As general oxidation hair dyes use strong acidic or alkaline components, there is a problem of damaging the hair by dissolving the hair components.Since dyeing occurs by oxidation polymerization inside the hair, the hair's gloss or gloss is lost and the hair is severely damaged. There is a problem that the texture deteriorates.

Therefore, studies on hair dyes that improve the condition of the scalp and hair by preventing various abnormal symptoms of the scalp and hair have been conducted, but there are many cases of side effects on the scalp as the main component is often a chemical substance.

Therefore, there is a need to develop a product that can deodorize dyes such as ammonia odor by antibacterial and inflammation relief, as well as improving the condition of damaged hair that is safe for the skin and has excellent formulation stability.

In particular, the odor of hair dye base such as ammonia odor can be approached by antibacterial and anti-inflammatory deodorant.

Antibacterial is largely divided into bactericidal and bacteriostatic by inhibiting the growth of bacteria and fungi by drugs or physiologically active substances. Anti-biotics are important for antibacterial activity.

The range of microorganisms that can cause antibiotics to exert antibacterial action is called the antibacterial zone.

It is a well-known fact that most bacterial infections have been suppressed by the advent of antibiotics, but the emergence and prevalence of drug-resistant bacteria that resist antibacterial action leaves a problem in treatment.

Compared to bacteria, there are few excellent antimicrobial agents that exhibit an action against fungi, but it is known that niastatin or cloplevin as antibiotics and salicylic acid, propionic acid, sorbate, etc. as synthetic products have antibacterial action against fungi.

Inflammation refers to a defensive reaction that occurs in the body when a living tissue is damaged. It is a medical concept and is defined by the Latin words calor, dolor, rubor, and tumor which mean fever, pain, redness, and swelling.

The causes of such inflammation include mechanical injury, physical factors such as temperature and radiation, chemical factors such as poisons, and parasites such as bacterial infection.

Recently, interest in deodorization and deodorization of odors generated from the human scalp is increasing.There are more than 100,000 types of odor-causing substances. In particular, amines, hydrogen sulfide, and other irritating gaseous substances are representative, and sweat secreted from the human body and Sebum is the cause of odor.

Of these, sebum secreted from the pores causes a peculiar odor when sweat or airborne bacteria or fungi are mixed, and the more it is secreted, the more inflamed it is, the weaker the inhibitory power against bacteria and the odor becomes worse.

Accordingly, a number of technologies have been proposed for hair dyes that exhibit a deodorizing effect based on natural raw materials.

In particular, cases of using natural herbal ingredients, which are known to contain various flavoring and antibacterial ingredients, have been disclosed.

For example, in "Tablet Hair-dye Composition Containing Natural Herb Powder" (Korean Patent Laid-Open No. 10-2018-0080412, Patent Document 1) discloses a case in which lemon glass powder is used as a hair-dye composition.

However, in Patent Document 1, only an experiment on the hair dyeing performance was conducted, and whether or not there is a deodorant or antibacterial effect due to the use of natural herbs has not been determined.

As another technology, "hypoallergenic hair dye containing natural substances" (Korean Patent Publication No. 10-1284607, Patent Document 2) discloses a hair dye composition containing a specific amount of lavender oil, a natural herb.

In Patent Document 2, there was only an experiment to confirm whether or not skin irritation caused by the use of the hair dye composition was used, but the deodorant performance or antibacterial performance was not determined.

On the other hand, "Hair-dye composition using a herbal complex" (Korean Patent Publication No. 10-1901422, Patent Document 3) discloses a technique for a hair-dye composition comprising lemon rice, lavender, and the like as raw materials.

The composition of Patent Document 3 was evaluated to have a deodorant effect and an effect of suppressing skin diseases.

However, there is no description of the type and individual content of the huff raw material added to Patent Document 3, as well as various other raw materials such as donkey skin extract, natural fermented mule, gardenia, beet, aronia, etc. It is unclear whether the B content alone achieves the effect of deodorizing and inhibiting skin diseases.

KR 10-2018-0080412 (2018.07.12) KR 10-1284607 (2013.07.04) KR 10-1901422 (2018.09.17)

The hair dye composition of the present invention having an effect on antibacterial, skin irritation alleviation, deodorant and ammonia odor removal is to solve the problems arising from the prior art as described above. Is to provide.

Another object of the present invention is to provide a multifunctional deodorant hair dye composition that is not cytotoxic, has little skin irritation, and is harmless to the human body.

Another object of the present invention is to provide an excellent composition for hair dyes that minimizes the base odor of ammonia odor by using lavender water, lemon balm leaf extract, lemon grass extract, and lavender oil.

Another object of the present invention is to provide a product using a composition for hair dye that meets the above.

The hair dye composition having an effect on antibacterial, skin irritation relief, deodorization and ammonia odor removal of the present invention is characterized in that it contains lavender water and lavender oil as active ingredients in order to solve the above problems.

In the above configuration, the lavender water and lavender oil is characterized in that it is included in a weight ratio of 1 to 50: 0.1 to 8.0.

In addition, it characterized in that it further comprises a lemon balm leaf extract and lemon grass extract as the active ingredient.

In addition, the lavender water, lemon balm leaf extract, and lemongrass extract are obtained by hot water extraction or organic solvent extraction, and the lavender oil is obtained by heating and distilling after mixing water.

The lavender water, lemon balm leaf extract, lemon grass extract, and lavender oil are included in a weight ratio of 1 to 50: 0.5 to 5.0: 0.5 to 5.0: 0.1 to 8.0.

In addition, the active ingredient is characterized in that it contains 2.1 to 68.0% by weight of the total weight of the hair dye composition.

The hair-dye composition according to the present invention is excellent in antibacterial and deodorant effects, is harmless to the human body due to little skin irritation, and is easily applied to hair products such as hair-dye.

In addition, it features a differentiated hair dye product with excellent antibacterial and skin irritation relieving functions, reducing preservatives and irritation alleviating agents, which are more economical than competitive hair dye products and have low manufacturing cost.

Figure 1 is a photograph showing the antibacterial effect on E. coli of the embodiment according to the present invention.
Figure 2 is a photograph showing the antibacterial effect on Pseudomonas aeruginosa of the embodiment according to the present invention.

Hereinafter, a hair dye composition having an effect on antibacterial, skin irritation relief, deodorant and ammonia odor removal of the present invention will be described in detail.

The hair-dye composition of the present invention comprises lavender water (Organic Lavender Water), lemon balm leaf extract (Organic Melissa Officinalis Leaf EXTRACT), lemon grass extract (Organic Cymbopogon Citratus Extract), lavender oil (Bulgarian Orgaic Lavender) as active ingredients.

The lavender water, lemon balm leaf extract, lemon grass extract, and lavender oil may be extracted using one or more of flowers, leaves, stems, and roots, and may be extracted including all parts.

In addition, in the present invention, the lavender, lemon balm leaves, and lemongrass may be divided or pulverized and mixed to extract a mixture, or each may be extracted, and both are extracted with hot water or an organic solvent, and the solvent is extracted with two different extraction methods. Can be easily extracted.

The extraction method is largely divided into three types: hot water, organic solvent, and supercritical. The hot water extraction method is filtered according to the liquid or powder result by adjusting the extraction conditions in the temperature range of 25 ~ 100℃ and the extraction time in the range of 6 ~ 12h. Add conditions such as centrifugation and cooling.

For organic solvent extraction, if various extractions are possible depending on the type of solvent such as butylene glycol, glycerin, ethylene glycol, ethanol, propylene glycol, etc., the formulation of the composition varies according to the antiseptic ability of the solvent. Commonly used organic solvents are butylene glycol and ethanol, but in the case of ethanol, the ethanol concentration (%) is in the range of 25 to 100%, the extraction temperature is in the range of 25 to 100°C, and the time is in the range of 6h to 12h. In comparison, the optimum yield can be extracted under conditions of good concentration, temperature, and time through a combination test, but the yield by conditions of the final result is related to economic productivity.

Supercritical extract is a method of injecting carbon dioxide while dropping the extract by using special equipment to break down the cell wall of the extract to extract the essential ingredients of the extract with high purity. Supercritical extraction is a low-temperature method unlike the above hot water or organic solvent extraction method. The advantage is that it can be extracted at low temperature without being limited to the destruction of nutrients by temperature depending on the type of natural product, but additional problems such as contamination of microorganisms can be improved through its own preservative power, but the economical efficiency of prescription such as cost Not suitable

Therefore, among the above three types, hot water and organic solvent extraction methods are preferable, and ethanol is suitable among the organic solvents.

Unlike the above extracts, in the case of oil, the extraction method is largely divided into the use of solvent and the non-solvent method. There are largely a cold precipitation method, a warm precipitation method, a solvent extraction method, a supercritical carbon dioxide extraction method, a steam distillation method, and a compression method. There are two methods: alcohol and hexane as a solvent and supercritical carbon dioxide extraction.

The extraction method without using a solvent includes a compression method in which the skin is compressed, and a steam distillation method in which the steam and oil molecules generated by heating the plant pass through a cooling tunnel to be divided into distillate and oil. Lavender oil requires solvent extraction using water. To use, the extraction method is to obtain oil by heating and distilling a mixture containing 4,000mL parts by weight of water in 200gr of natural products using natural products of lavender at 80~120℃ for 24~48 hours.

In the present invention, the above hot water extraction method uses natural products such as lavender, lemon balm leaves, and lemongrass to obtain an extract by heating a mixture containing 4,000 mL parts by weight of water to 100 g of natural products at 50 to 100°C for 12 to 24 hours. This extraction was extracted by heating at 100° C. for 8 hours or more, and the extract components of the above lavender, lemon balm leaves, and lemongrass were not damaged.

After the above hot water extraction, various results can be obtained by filtering, concentrating, and drying using reflux cooling extraction, ultrasonic extraction, cold sediment extraction, etc.

In the present invention, the above organic solvent extraction is the above organic solvent extraction 100 parts by weight of each natural product pulverized to make the above lavender water, lemon balm leaf extract, lemon grass extract, and 4,000 mL of a water mixture solvent in 50% of ethanol as an organic solvent. It is extracted by heating at 100° C. from the mixture containing the organic solvent, and the organic solvent is characterized by selecting and extracting an alcohol among ethanol, glycerin, propylene glycol, butylene glycol, and hexane.

The alcohol used in the alcohol extract is characterized by using ethyl alcohol among ethyl alcohol, propyl alcohol, benzyl alcohol and poly alcohol.

The ethanol concentration (%) is in the range of 25 to 100%, the extraction temperature is in the range of 25 to 100°C, and the time is in the range of 6h to 12h. After solvent extraction, using reflux cooling extraction, ultrasonic extraction, cold sediment extraction, etc., various results of lavender water, lemon balm leaf extract, lemongrass extract and lavender oil that are not damaged by filtration, concentration, and drying can be obtained.

When the above ingredients such as lavender, lemon rice, and lemongrass are processed in powder form, the optimum dissolution conditions cannot be satisfied in various formulations of hair dye such as cream or gel type, and even if the content of the effective range is dissolved, exposure at low temperatures for a long time When re-precipitated, it becomes an unsuitable factor in the stability of the formulation. Therefore, components extracted by hot water and organic solvent extraction methods must be used.

In the present invention, when lemon balm leaf, lemongrass extract, lavender water and lavender oil with excellent antibacterial and deodorant functions are contained in the composition with a synergistic effect, the antibacterial and deodorant effect is increased, inflammation is improved, and a hair dye composition with excellent deodorizing effect can be made. Compared with the products having the effect, it is possible to manufacture excellent products of economic productivity with a small number of mixtures.

Hereinafter, specific examples according to the present invention, comparative examples and experimental examples for comparison with the examples will be described.

Lavender water, lemon balm leaf extract, and lemongrass extract specified in the examples below are prepared by pulverizing with a pulverizer after washing foreign substances of raw materials, and then containing 4,000 mL parts by weight of water in 100 g of pulverized products for each raw material. The mixture was heated at 100° C. for 12 to 24 h or more to obtain hot water extraction.

At this time, a mixture containing 4,000 mL of a water-mixed solvent in 50% of organic solvent ethanol per 100 g of the pulverized product may be obtained by heating at 100° C. for 8 hours.

In addition, lavender oil specified in the examples below was obtained by distilling and heating a mixture containing 4,000 mL parts by weight of water to 200 g of lavender for 24 to 48 h at 80 to 120°C.

In order to compare the effects of the mixed extract of the present invention, the effect was dataed by comparing Examples and Comparative Examples with a cream-type hair-dye formulation and a gel-type shampoo-type hair-dye formulation. First, the examples and comparative examples below are comparative data for cream type hair dyes.

<Preparation of Examples 1 and 2 and Comparative Examples 1 to 4>

The hair dyes of Examples 1 to 2 and Comparative Examples 1 to 4 were prepared based on the ingredients and contents of the cream type hair dye prepared in Table 1 below.

In the manufacturing process, after heating the phases A and B in Table 1 to 85°C, homomixing emulsification (RPM: 3,000 rpm, Time: 10 minutes, cooling to 50°C) to make a homogeneous phase, and then add phase C. And homomixing emulsification (RPM: 2,000 rpm, Time: 10 minutes degassing and cooling to complete 30°C).

<Manufacture of cream type hair dye> Ingredient/content (%) Classification (top) Example Comparative example One 2 One 2 3 4 Purified water A phase Balance Balance Balance Balance Balance Balance Allantoin 0.5 0.5 0.5 0.5 0.5 0.5 glycerin 0.1 0.1 0.1 0.1 0.1 0.1 Polyoxyethylene lauryl ether B phase 2.5 2.5 2.5 2.5 2.5 2.5 Polyoxyethylene oleyl ether 1.5 1.5 1.5 1.5 1.5 1.5 Cetostearyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 Cetyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 p-aminophenol 1.0 1.0 1.0 1.0 1.0 1.0 Resorcinol 1.0 1.0 1.0 1.0 1.0 1.0 Monoethanolamine 0.6 0.6 0.6 0.6 0.6 0.6 ammonia 3.5 3.5 3.5 3.5 3.5 3.5 Hydrolyzed Collagen C phase 1.0 1.0 1.0 1.0 1.0 1.0 Lavender water 1.0 1.0 - 1.0 1.0 1.0 Lemongrass extract 0.5 0.5 0.5 - 0.5 0.5 Lemon Balm Leaf Extract 0.5 0.5 0.5 0.5 - 0.5 Lavender oil 0.1 0.2 0.1 0.1 0.1 - Hydrogen peroxide (30%) 17.0 17.0 17.0 17.0 17.0 17.0 Phosphoric acid 0.1 0.1 0.1 0.1 0.1 0.1

<Experimental Example 1> Skin irritation test

In order to investigate the skin irritation test for the hair dyes of Examples 1 to 2 and Comparative Examples 1 to 4 above, a skin patch test was performed.

The population was selected based on people aged 30 to 60 without skin disease.

The number of people selected was 10 people, and the patch area was examined by wiping the upper arm of the left arm with ethanol to remove microorganisms, drying for 30 minutes, applying 1 g of cream hair products for 48 hours each, and comparing by time.

The skin reaction results were judged according to the following criteria, and the patch test results are shown in Table 2.

<Standard for skin reaction test>

- : no response

≠: Weak positivity

+: Positive reaction (erythema)

++: Strong positive reaction (erythema, edema)

+++: Positive cardiac response (erythema, edema, blisters)

<Skin reaction judgment result> Prescription Number of testers Judgment result - ≠ + ++ +++ Example 1 20.0 20.0 0 0 0 0 Example 2 20.0 20.0 0 0 0 0 Comparative Example 1 20.0 19.0 One 0 0 0 Comparative Example 2 20.0 18.0 One One 0 0 Comparative Example 3 20.0 18.0 One One 0 0 Comparative Example 4 20.0 18.0 One One 0 0

Looking at the results in Table 2 above, it can be seen that when comparing the compositions of Examples 1 and 2 and Comparative Examples, Comparative Examples 1 to 4 have more stimulation than Examples 1 and 2.

That is, each of Comparative Examples 1 to 4 is missing one of the four components, and in this case, it can be seen that the effect of improving stimulation is poor.

<Preparation of Examples 3 and 4 and Comparative Examples 5 to 8>

Hair dyes of Examples 3 to 4 and Comparative Examples 5 to 8 were prepared based on the ingredients and contents of the cream type hair dye prepared in Table 3 below.

In the manufacturing process, after heating the phases A and B in Table 3 to 85℃, homomixing emulsification (RPM: 3,000rpm, Time: 10 minutes, cooling to 50℃) to make a homogeneous phase, and then phase C was added. And homomixing emulsification (RPM: 2,000 rpm, Time: 10 minutes degassing and cooling to complete 30°C).

<Manufacture of cream type hair dye> Ingredient/content (%) Classification (top) Example Comparative example 3 4 5 6 7 8 Purified water A phase Balance Balance Balance Balance Balance Balance Allantoin 0.5 0.5 0.5 0.5 0.5 0.5 glycerin 0.1 0.1 0.1 0.1 0.1 0.1 Polyoxyethylene lauryl ether B phase 2.5 2.5 2.5 2.5 2.5 2.5 Polyoxyethylene oleyl ether 1.5 1.5 1.5 1.5 1.5 1.5 Cetostearyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 Cetyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 p-aminophenol 1.0 1.0 1.0 1.0 1.0 1.0 Resorcinol 1.0 1.0 1.0 1.0 1.0 1.0 Monoethanolamine 0.6 0.6 0.6 0.6 0.6 0.6 ammonia 3.5 3.5 3.5 3.5 3.5 3.5 Hydrolyzed Collagen C phase 1.0 1.0 1.0 1.0 1.0 1.0 Lavender water 2.0 3.0 0.9 1.0 1.0 1.0 Lemongrass extract 0.5 0.5 0.5 0.4 0.5 0.5 Lemon Balm Leaf Extract 0.5 0.5 0.5 0.5 0.4 0.5 Lavender oil 0.1 0.1 1.0 1.0 1.0 0.09 Hydrogen peroxide (30%) 17.0 17.0 17.0 17.0 17.0 17.0 Phosphoric acid 0.1 0.1 0.1 0.1 0.1 0.1

<Experimental Example 2> Skin irritation test

The hair dyes of Examples 3 to 4 and Comparative Examples 5 to 8 were subjected to a skin irritation experiment in the same manner as in Experimental Example 1, and the results are shown in Table 4 below.

<Skin reaction judgment result> Prescription Number of testers Judgment result - ≠ + ++ +++ Example 3 20.0 20.0 0 0 0 0 Example 4 20.0 20.0 0 0 0 0 Comparative Example 5 20.0 18.0 One One 0 0 Comparative Example 6 20.0 18.0 One One 0 0 Comparative Example 7 20.0 18.0 One One 0 0 Comparative Example 8 20.0 18.0 One One 0 0

Looking at the results in Table 4 above, it can be seen that Comparative Examples 5 to 8 have more irritation than Examples 3 to 4 when comparing the hair dye composition of Examples 3 and 4 and Comparative Examples.

That is, in each of Comparative Examples 5 to 8, it can be seen that one of the four components is out of the reference range and there is no effect of improving stimulation.

<Preparation of Examples 5 and 6 and Comparative Examples 9 to 12>

Hair dyes of Examples 5 to 6 and Comparative Examples 9 to 12 were prepared based on the components and contents of the cream-type hair dye prepared in Table 5 below.

In the manufacturing process, after heating the phases A and B in Table 3 to 85℃, homomixing emulsification (RPM: 3,000rpm, Time: 10 minutes, cooling to 50℃) to make a homogeneous phase, and then phase C was added. And homomixing emulsification (RPM: 2,000 rpm, Time: 10 minutes degassing and cooling to complete 30°C).

<Manufacture of cream type hair dye> Ingredient/content (%) Classification (top) Example Comparative example 5 6 9 10 11 12 Purified water A phase Balance Balance Balance Balance Balance Balance Allantoin 0.5 0.5 0.5 0.5 0.5 0.5 glycerin 0.1 0.1 0.1 0.1 0.1 0.1 Polyoxyethylene lauryl ether B phase 2.5 2.5 2.5 2.5 2.5 2.5 Polyoxyethylene oleyl ether 1.5 1.5 1.5 1.5 1.5 1.5 Cetostearyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 Cetyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 p-aminophenol 1.0 1.0 1.0 1.0 1.0 1.0 Resorcinol 1.0 1.0 1.0 1.0 1.0 1.0 Monoethanolamine 0.6 0.6 0.6 0.6 0.6 0.6 ammonia 3.5 3.5 3.5 3.5 3.5 3.5 Hydrolyzed Collagen C phase 1.0 1.0 1.0 1.0 1.0 1.0 Lavender water 4.0 5.0 0.9 0.9 0.9 1.0 Lemongrass extract 1.0 1.0 0.4 0.5 0.5 0.4 Lemon Balm Leaf Extract 1.0 1.0 1.0 0.4 1.0 0.4 Lavender oil 0.4 0.4 0.5 0.5 0.09 0.1 Hydrogen peroxide (30%) 17.0 17.0 17.0 17.0 17.0 17.0 Phosphoric acid 0.1 0.1 0.1 0.1 0.1 0.1

<Experimental Example 3> Skin irritation test

The hair dyes of Examples 5 to 6 and Comparative Examples 9 to 12 were subjected to a skin irritation experiment in the same manner as in Experimental Example 1, and the results are shown in Table 6 below.

<Skin irritation judgment result> Prescription Number of testers Judgment result - ≠ + ++ +++ Example 5 20.0 20.0 0 0 0 0 Example 6 20.0 20.0 0 0 0 0 Comparative Example 9 20.0 18.0 One One 0 0 Comparative Example 10 20.0 19.0 0 One 0 0 Comparative Example 11 20.0 18.0 One One 0 0 Comparative Example 12 20.0 18.0 One One 0 0

Looking at the results in Table 6 above, it can be seen that when comparing the hair dye composition of the Example and Comparative Example of the present composition patent, Comparative Examples 9 to 12 have more irritation than Examples 5 to 6.

That is, in Comparative Examples 9 to 12, it can be seen that among the four ingredients, lavender water and lavender oil are outside the standard range of effects and have no effect of improving stimulation.

<Preparation of Examples 7, 8 and Comparative Examples 13 to 16>

The hair dyes of Examples 7 to 8 and Comparative Examples 9 to 12 were prepared based on the ingredients and contents of the cream type hair dye prepared in Table 7 below.

In the manufacturing process, after heating the phases A and B in Table 3 to 85℃, homomixing emulsification (RPM: 3,000rpm, Time: 10 minutes, cooling to 50℃) to make a homogeneous phase, and then phase C was added. And homomixing emulsification (RPM: 2,000 rpm, Time: 10 minutes degassing and cooling to complete 30°C).

<Manufacture of cream type hair dye> Ingredient/content (%) Classification (top) Example Comparative example 7 8 13 14 15 16 Purified water A phase Balance Balance Balance Balance Balance Balance Allantoin 0.5 0.5 0.5 0.5 0.5 0.5 glycerin 0.1 0.1 0.1 0.1 0.1 0.1 Polyoxyethylene lauryl ether B phase 2.5 2.5 2.5 2.5 2.5 2.5 Polyoxyethylene oleyl ether 1.5 1.5 1.5 1.5 1.5 1.5 Cetostearyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 Cetyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 p-aminophenol 1.0 1.0 1.0 1.0 1.0 1.0 Resorcinol 1.0 1.0 1.0 1.0 1.0 1.0 Monoethanolamine 0.6 0.6 0.6 0.6 0.6 0.6 ammonia 3.5 3.5 3.5 3.5 3.5 3.5 Hydrolyzed Collagen C phase 1.0 1.0 1.0 1.0 1.0 1.0 Lavender water 5.0 5.0 0.5 0.6 3.0 4.0 Lemongrass extract 2.0 2.0 2.0 3.0 0.4 0.4 Lemon Balm Leaf Extract 2.0 2.0 2.0 3.0 0.4 0.4 Lavender oil 0.6 0.7 0.09 0.09 0.6 0.9 Hydrogen peroxide (30%) 17.0 17.0 17.0 17.0 17.0 17.0 Phosphoric acid 0.1 0.1 0.1 0.1 0.1 0.1

<Experimental Example 4> Skin irritation test

The hair dyes of Examples 7 to 8 and Comparative Examples 13 to 16 were subjected to a skin irritation experiment in the same manner as in Experimental Example 1, and the results are shown in Table 8 below.

<Skin irritation judgment result> Prescription Number of testers Judgment result - ≠ + ++ +++ Example 7 20.0 20.0 0 0 0 0 Example 8 20.0 20.0 0 0 0 0 Comparative Example 13 20.0 18.0 One One 0 0 Comparative Example 14 20.0 19.0 0 One 0 0 Comparative Example 15 20.0 18.0 One One 0 0 Comparative Example 16 20.0 19.0 One 0 0 0

Looking at the results in Table 8 above, it can be seen that Comparative Examples 13 to 16 have more irritation than Examples 7 to 8 when comparing the hair dye composition of Examples and Comparative Examples.

In other words, it can be seen that in each of Comparative Examples 13 to 16, the lemon balm leaf extract and lemon grass extract out of the reference range of the effect were not effective in improving stimulation.

<Preparation of Examples 9 and 10 and Comparative Examples 17 to 20>

The hair dyes of Examples 9 to 10 and Comparative Examples 17 to 20 were prepared based on the ingredients and contents of the cream-type hair dye prepared in Table 9 below.

In the manufacturing process, after heating the phases A and B in Table 3 to 85℃, homomixing emulsification (RPM: 3,000rpm, Time: 10 minutes, cooling to 50℃) to make a homogeneous phase, and then phase C was added. And homomixing emulsification (RPM: 2,000 rpm, Time: 10 minutes degassing and cooling to complete 30°C).

<Manufacture of cream type hair dye> Ingredient/content (%) Classification (top) Example Comparative example 9 10 17 18 19 20 Purified water A phase Balance Balance Balance Balance Balance Balance Allantoin 0.5 0.5 0.5 0.5 0.5 0.5 glycerin 0.1 0.1 0.1 0.1 0.1 0.1 Polyoxyethylene lauryl ether B phase 2.5 2.5 2.5 2.5 2.5 2.5 Polyoxyethylene oleyl ether 1.5 1.5 1.5 1.5 1.5 1.5 Cetostearyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 Cetyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 p-aminophenol 1.0 1.0 1.0 1.0 1.0 1.0 Resorcinol 1.0 1.0 1.0 1.0 1.0 1.0 Monoethanolamine 0.6 0.6 0.6 0.6 0.6 0.6 ammonia 3.5 3.5 3.5 3.5 3.5 3.5 Hydrolyzed Collagen C phase 1.0 1.0 1.0 1.0 1.0 1.0 Lavender water 10.0 20.0 51.0 40.0 30.0 20.0 Lemongrass extract 3.0 3.0 4.0 6.0 3.0 3.5 Lemon Balm Leaf Extract 3.0 3.0 4.0 3.0 6.0 4.5 Lavender oil 8.0 7.0 8.0 6.0 8.0 8.1 Hydrogen peroxide (30%) 17.0 17.0 17.0 17.0 17.0 17.0 Phosphoric acid 0.1 0.1 0.1 0.1 0.1 0.1

<Experimental Example 5> Skin irritation test

The hair dyes of Examples 9 to 10 and Comparative Examples 17 to 20 were subjected to a skin irritation experiment in the same manner as in Experimental Example 1, and the results are shown in Table 10 below.

<Skin irritation judgment result> Prescription Number of testers Judgment result - ≠ + ++ +++ Example 9 20.0 20.0 0 0 0 0 Example 10 20.0 20.0 0 0 0 0 Comparative Example 17 20.0 18.0 One One 0 0 Comparative Example 18 20.0 19.0 One 0 0 0 Comparative Example 19 20.0 18.0 One One 0 0 Comparative Example 20 20.0 19.0 One 0 0 0

Looking at the results in Table 10 above, it can be seen that when comparing the hair dye composition of the Example and the Comparative Example, Comparative Examples 17 to 20 have more irritation than Examples 9 to 10.

That is, each of the four components of Comparative Examples 17 to 20 was found that there was no effect of improving stimulation outside the standard range of the effect to the higher the effective content of lavender water 51: lemongrass extract 6: lemon balm leaf extract 6: lavender oil 8.1. I can.

<Preparation of Examples 11 and 12 and Comparative Examples 21 to 24>

Hair dyes of Examples 11 to 12 and Comparative Examples 21 to 24 were prepared based on the components and contents of the cream type hair dye prepared in Table 9 below.

In the manufacturing process, after heating the phases A and B in Table 3 to 85℃, homomixing emulsification (RPM: 3,000rpm, Time: 10 minutes, cooling to 50℃) to make a homogeneous phase, and then phase C was added. And homomixing emulsification (RPM: 2,000 rpm, Time: 10 minutes degassing and cooling to complete 30°C).

<Manufacture of cream type hair dye> Ingredient/content (%) Classification (top) Example Comparative example 11 12 21 22 23 24 Purified water A phase Balance Balance Balance Balance Balance Balance Allantoin 0.5 0.5 0.5 0.5 0.5 0.5 glycerin 0.1 0.1 0.1 0.1 0.1 0.1 Polyoxyethylene lauryl ether B phase 2.5 2.5 2.5 2.5 2.5 2.5 Polyoxyethylene oleyl ether 1.5 1.5 1.5 1.5 1.5 1.5 Cetostearyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 Cetyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 p-aminophenol 1.0 1.0 1.0 1.0 1.0 1.0 Resorcinol 1.0 1.0 1.0 1.0 1.0 1.0 Monoethanolamine 0.6 0.6 0.6 0.6 0.6 0.6 ammonia 3.5 3.5 3.5 3.5 3.5 3.5 Hydrolyzed Collagen C phase 1.0 1.0 1.0 1.0 1.0 1.0 Lavender water 30.0 40.0 50.5 10.5 50.1 50.0 Lemongrass extract 4.0 2.0 5.1 4.5 5.0 5.1 Lemon Balm Leaf Extract 4.0 2.0 3.0 5.1 5.1 5.0 Lavender oil 5.0 4.0 8.0 8.1 8.0 8.1 Hydrogen peroxide (30%) 17.0 17.0 17.0 17.0 17.0 17.0 Phosphoric acid 0.1 0.1 0.1 0.1 0.1 0.1

<Experimental Example 6> Skin irritation test

The hair dyes of Examples 11 to 12 and Comparative Examples 21 to 24 were subjected to a skin irritation experiment in the same manner as in Experimental Example 1, and the results are shown in Table 12 below.

<Skin irritation judgment result> Prescription Number of testers Judgment result - ≠ + ++ +++ Example 11 20.0 20.0 0 0 0 0 Example 12 20.0 20.0 0 0 0 0 Comparative Example 21 20.0 18.0 One One 0 0 Comparative Example 22 20.0 18.0 One One 0 0 Comparative Example 23 20.0 18.0 One One 0 0 Comparative Example 24 20.0 19.0 One 0 0 0

Looking at the results in Table 12 above, it can be seen that when comparing the hair dye composition of the Example and the Comparative Example, Comparative Examples 21 to 24 have more irritation than Examples 11 to 12.

That is, it can be seen that two or more of the four components of Comparative Examples 21 to 24 have a higher content, respectively, out of the effect reference range, and there is no effect of stimulation improvement.

<Preparation of Examples 13 and 14 and Comparative Examples 25 to 28>

Hair dyes of Examples 13 to 14 and Comparative Examples 25 to 28 were prepared based on the ingredients and contents of the cream type hair dye prepared in Table 13 below.

In the manufacturing process, after heating the phases A and B in Table 3 to 85℃, homomixing emulsification (RPM: 3,000rpm, Time: 10 minutes, cooling to 50℃) to make a homogeneous phase, and then phase C was added. And homomixing emulsification (RPM: 2,000 rpm, Time: 10 minutes degassing and cooling to complete 30°C).

<Manufacture of cream type hair dye> Ingredient/content (%) Classification (top) Example Comparative example 13 14 25 26 27 28 Purified water A phase Balance Balance Balance Balance Balance Balance Allantoin 0.5 0.5 0.5 0.5 0.5 0.5 glycerin 0.1 0.1 0.1 0.1 0.1 0.1 Polyoxyethylene lauryl ether B phase 2.5 2.5 2.5 2.5 2.5 2.5 Polyoxyethylene oleyl ether 1.5 1.5 1.5 1.5 1.5 1.5 Cetostearyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 Cetyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 p-aminophenol 1.0 1.0 1.0 1.0 1.0 1.0 Resorcinol 1.0 1.0 1.0 1.0 1.0 1.0 Monoethanolamine 0.6 0.6 0.6 0.6 0.6 0.6 ammonia 3.5 3.5 3.5 3.5 3.5 3.5 Hydrolyzed Collagen C phase 1.0 1.0 1.0 1.0 1.0 1.0 Lavender water 50.0 50.0 50.1 20.0 50.1 25.0 Lemongrass extract 2.0 3.0 1.5 5.1 5.1 5.0 Lemon Balm Leaf Extract 3.0 2.0 2.0 5.1 5.0 5.1 Lavender oil 5.0 8.0 8.1 8.0 8.0 8.1 Hydrogen peroxide (30%) 17.0 17.0 17.0 17.0 17.0 17.0 Phosphoric acid 0.1 0.1 0.1 0.1 0.1 0.1

<Experimental Example 7> Skin irritation test

The hair dyes of Examples 13 to 14 and Comparative Examples 25 to 28 were subjected to a skin irritation experiment in the same manner as in Experimental Example 1, and the results are shown in Table 13 below.

<Skin irritation judgment result> Prescription Number of testers Judgment result - ≠ + ++ +++ Example 13 20.0 20.0 0 0 0 0 Example 14 20.0 20.0 0 0 0 0 Comparative Example 25 20.0 18.0 One One 0 0 Comparative Example 26 20.0 18.0 One One 0 0 Comparative Example 27 20.0 18.0 One One 0 0 Comparative Example 28 20.0 18.0 One One 0 0

Looking at the results in Table 14 above, it can be seen that Comparative Examples 25 to 28 have more irritation than Examples 13 to 14 when comparing the hair dye composition of the Example and Comparative Example.

That is, out of the four ingredients of Comparative Examples 25 to 28, two or more of lavender water, lavender oil, lemon balm leaf extract, and lemongrass extract each had a higher content, respectively, out of the reference range of the effect, and there was no effect of improving stimulation.

In order to show the extract effect data for each formulation of the present invention, the second gel (liquid) type (shampoo-type hair dye) Comparative Example and Example were compared as follows.This formulation is a fast dyeable fast hair dye within 1 to 10 minutes. Dyeing is possible.

<Examples 15 and 16 Preparation of Comparative Examples 29 to 32>

The hair dyes of Examples 15 to 16 and Comparative Examples 29 to 32 were prepared based on the ingredients and contents of the gel-type hair dye prepared in Table 15 below.

In the manufacturing process, after heating the phases A and B in Table 15 to 85℃, homomixing and emulsifying (RPM: 3,000rpm, Time: 10 minutes, cooling to 50℃) to make a homogeneous phase, and then introducing phase C. And homomixing emulsification (RPM: 2,000 rpm, Time: 10 minutes degassing and cooling to complete 30°C).

<Manufacture of gel type (shampoo type) hair dye> Ingredient/content (%) Classification (top) Example Comparative example 15 16 29 30 31 32 Purified water A phase Balance Balance Balance Balance Balance Balance Propylene glycol 5.0 5.0 5.0 5.0 5.0 5.0 Sodium laureth sulfate 5.0 5.0 5.0 5.0 5.0 5.0 Disodium EDTA 0.1 0.1 0.1 0.1 0.1 0.1 Sodium lauryl sulfate 5.0 5.0 5.0 5.0 5.0 5.0 Ethylhexyloctanoate B phase 1.7 1.7 1.7 1.7 1.7 1.7 Ethylhexylstearate 5.0 5.0 5.0 5.0 5.0 5.0 Cocamide D.A. 7.0 7.0 7.0 7.0 7.0 7.0 Oleic Acid 7.0 7.0 7.0 7.0 7.0 7.0 Steatrimonium chloride 3.5 3.5 3.5 3.5 3.5 3.5 Stearyl alcohol 1.0 1.0 1.0 1.0 1.0 1.0 Cetyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 Lauryl alcohol 1.0 1.0 1.0 1.0 1.0 1.0 p-aminophenol C phase 0.35 0.35 0.35 0.35 0.35 0.35 p-phenylenediamine 2.1 2.1 2.1 2.1 2.1 2.1 o-aminophenol 0.05 0.05 0.05 0.05 0.05 0.05 m-aminophenol 0.6 0.6 0.6 0.6 0.6 0.6 Ammonium thioglyconate 0.5 0.5 0.5 0.5 0.5 0.5 Sodium astorbate 0.3 0.3 0.3 0.3 0.3 0.3 Dry sodium sulfite 0.9 0.9 0.9 0.9 0.9 0.9 Monoethanolamine 2.8 2.8 2.8 2.8 2.8 2.8 ammonia 3.0 3.0 3.0 3.0 3.0 3.0 Sodium chloride 0.5 0.5 0.5 0.5 0.5 0.5 Resorcin 0.8 0.8 0.8 0.8 0.8 0.8 Lavender water 1.0 1.0 - 1.0 1.0 1.0 Lemongrass extract 0.5 0.5 0.5 - 0.5 0.5 Lemon Balm Leaf Extract 0.5 0.5 0.5 0.5 - 0.5 Lavender oil 0.1 0.2 0.1 0.1 0.1 - Sodium Thioglyconate 15.0 15.0 15.0 15.0 15.0 15.0 Hydrolyzed Collagen 1.0 1.0 1.0 1.0 1.0 1.0

<Experimental Example 8> Skin irritation test

In order to investigate the skin irritation test for the hair dyes of Examples 15 to 16 and Comparative Examples 29 to 32 above, a skin patch test was performed.

The population was selected based on people aged 30 to 60 without skin disease.

The number of people selected was 10 people, and the patch area was examined by wiping the upper arm of the left arm with ethanol to remove microorganisms, drying for 30 minutes, applying 1 g of gel-type hair dye products for 48 hours each, and comparing by time.

The skin reaction result was judged according to the following criteria, and the patch test results are shown in Table 16.

<Standard for skin reaction test>

- : no response

≠: Weak positivity

+: Positive reaction (erythema)

++: Strong positive reaction (erythema, edema)

+++: Positive cardiac response (erythema, edema, blisters)

<Skin reaction judgment result> Prescription Number of testers Judgment result - ≠ + ++ +++ Example 15 20.0 20.0 0 0 0 0 Example 16 20.0 20.0 0 0 0 0 Comparative Example 29 20.0 19.0 One 0 0 0 Comparative Example 30 20.0 19.0 0 One 0 0 Comparative Example 31 20.0 18.0 One One 0 0 Comparative Example 32 20.0 18.0 One One 0 0

Looking at the results of Table 16 above, it can be seen that when comparing the compositions of Examples and Comparative Examples, Comparative Examples 29 to 32 have more stimulation than Examples 19 to 20.

That is, each of the comparative examples is one of the four components, but in this case, it can be seen that the effect of improving stimulation is poor.

<Preparation of Examples 17 and 18 and Comparative Examples 33 to 36>

The hair dyes of Examples 17 to 18 and Comparative Examples 33 to 36 were prepared based on the ingredients and contents of the gel type (shampoo type) hair dye prepared in Table 17 below.

In the manufacturing process, after heating the phases A and B in Table 3 to 85℃, homomixing emulsification (RPM: 3,000rpm, Time: 10 minutes, cooling to 50℃) to make a homogeneous phase, and then phase C was added. And homomixing emulsification (RPM: 2,000 rpm, Time: 10 minutes degassing and cooling to complete 30°C).

<Manufacture of gel type (shampoo type) hair dye> Ingredient/content (%) Classification (top) Example Comparative example 17 18 33 34 35 36 Purified water A phase Balance Balance Balance Balance Balance Balance Propylene glycol 5.0 5.0 5.0 5.0 5.0 5.0 Sodium laureth sulfate 5.0 5.0 5.0 5.0 5.0 5.0 Disodium EDTA 0.1 0.1 0.1 0.1 0.1 0.1 Sodium lauryl sulfate 5.0 5.0 5.0 5.0 5.0 5.0 Ethylhexyloctanoate B phase 1.7 1.7 1.7 1.7 1.7 1.7 Ethylhexylstearate 5.0 5.0 5.0 5.0 5.0 5.0 Cocamide D.A. 7.0 7.0 7.0 7.0 7.0 7.0 Oleic Acid 7.0 7.0 7.0 7.0 7.0 7.0 Steatrimonium chloride 3.5 3.5 3.5 3.5 3.5 3.5 Stearyl alcohol 1.0 1.0 1.0 1.0 1.0 1.0 Cetyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 Lauryl alcohol 1.0 1.0 1.0 1.0 1.0 1.0 p-aminophenol C phase 0.35 0.35 0.35 0.35 0.35 0.35 p-phenylenediamine 2.1 2.1 2.1 2.1 2.1 2.1 o-aminophenol 0.05 0.05 0.05 0.05 0.05 0.05 m-aminophenol 0.6 0.6 0.6 0.6 0.6 0.6 Ammonium thioglyconate 0.5 0.5 0.5 0.5 0.5 0.5 Sodium astorbate 0.3 0.3 0.3 0.3 0.3 0.3 Dry sodium sulfite 0.9 0.9 0.9 0.9 0.9 0.9 Monoethanolamine 2.8 2.8 2.8 2.8 2.8 2.8 ammonia 3.0 3.0 3.0 3.0 3.0 3.0 Sodium chloride 0.5 0.5 0.5 0.5 0.5 0.5 Resorcin 0.8 0.8 0.8 0.8 0.8 0.8 Lavender water 2.0 3.0 0.9 1.0 1.0 1.0 Lemongrass extract 0.5 0.5 0.5 0.4 0.5 0.5 Lemon Balm Leaf Extract 0.5 0.5 0.5 0.5 0.4 0.5 Lavender oil 0.1 0.1 1.0 1.0 1.0 0.09 Sodium Thioglyconate 12.0 12.0 12.0 12.0 12.0 12.0 Hydrolyzed Collagen 1.0 1.0 1.0 1.0 1.0 1.0

<Experimental Example 9> Skin irritation test

The hair dyes of Examples 17 to 18 and Comparative Examples 33 to 36 were subjected to a skin irritation experiment in the same manner as in Experimental Example 1, and the results are shown in Table 10 below.

<Skin reaction judgment result> Prescription Number of testers Judgment result - ≠ + ++ +++ Example 17 20.0 20.0 0 0 0 0 Example 18 20.0 20.0 0 0 0 0 Comparative Example 33 20.0 18.0 One One 0 0 Comparative Example 34 20.0 18.0 One One 0 0 Comparative Example 35 20.0 19.0 One 0 0 0 Comparative Example 36 20.0 18.0 One One 0 0

Looking at the results in Table 18 above, it can be seen that when comparing the hair dye composition of Examples and Comparative Examples, Comparative Examples 33 to 36 have more irritation than Examples 17 to 18.

That is, it can be seen that in each of Comparative Examples 33 to 36, one of the four components is out of the reference range and there is no effect of stimulation improvement.

<Preparation of Examples 19 and 20 and Comparative Examples 37 to 40>

The hair dyes of Examples 19 to 20 and Comparative Examples 37 to 40 were prepared based on the ingredients and contents of the gel-type hair dye prepared in Table 19 below.

In the manufacturing process, after heating the phases A and B in Table 3 to 85℃, homomixing emulsification (RPM: 3,000rpm, Time: 10 minutes, cooling to 50℃) to make a homogeneous phase, and then phase C was added. And homomixing emulsification (RPM: 2,000 rpm, Time: 10 minutes degassing and cooling to complete 30°C).

<Gel type (shampoo type) hair dye manufacturing> Ingredient/content (%) Classification (top) Example Comparative example 19 20 37 38 39 40 Purified water A phase Balance Balance Balance Balance Balance Balance Propylene glycol 5.0 5.0 5.0 5.0 5.0 5.0 Sodium laureth sulfate 5.0 5.0 5.0 5.0 5.0 5.0 Disodium EDTA 0.1 0.1 0.1 0.1 0.1 0.1 Sodium lauryl sulfate 5.0 5.0 5.0 5.0 5.0 5.0 Ethylhexyloctanoate B phase 1.7 1.7 1.7 1.7 1.7 1.7 Ethylhexylstearate 5.0 5.0 5.0 5.0 5.0 5.0 Cocamide D.A. 7.0 7.0 7.0 7.0 7.0 7.0 Oleic Acid 7.0 7.0 7.0 7.0 7.0 7.0 Steatrimonium chloride 3.5 3.5 3.5 3.5 3.5 3.5 Stearyl alcohol 1.0 1.0 1.0 1.0 1.0 1.0 Cetyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 Lauryl alcohol 4.0 4.0 4.0 4.0 4.0 4.0 p-aminophenol C phase 0.35 0.35 0.35 0.35 0.35 0.35 p-phenylenediamine 2.1 2.1 2.1 2.1 2.1 2.1 o-aminophenol 0.05 0.05 0.05 0.05 0.05 0.05 m-aminophenol 0.6 0.6 0.6 0.6 0.6 0.6 Ammonium thioglyconate 0.5 0.5 0.5 0.5 0.5 0.5 Sodium astorbate 0.3 0.3 0.3 0.3 0.3 0.3 Dry sodium sulfite 0.9 0.9 0.9 0.9 0.9 0.9 Monoethanolamine 2.8 2.8 2.8 2.8 2.8 2.8 ammonia 3.0 3.0 3.0 3.0 3.0 3.0 Sodium chloride 0.5 0.5 0.5 0.5 0.5 0.5 Resorcin 0.8 0.8 0.8 0.8 0.8 0.8 Lavender water 4.0 5.0 0.9 0.9 0.9 1.0 Lemongrass extract 1.0 1.0 0.4 0.5 0.5 0.4 Lemon Balm Leaf Extract 1.0 1.0 1.0 0.4 1.0 0.4 Lavender oil 0.4 0.4 0.5 0.5 0.09 0.1 Sodium Thioglyconate 12.0 12.0 12.0 12.0 12.0 12.0 Hydrolyzed Collagen 1.0 1.0 1.0 1.0 1.0 1.0

<Experimental Example 10> Skin irritation test

The hair dyes of Examples 19 to 20 and Comparative Examples 37 to 40 above were subjected to a skin irritation experiment in the same manner as in Experimental Example 1, and the results are shown in Table 6 below.

<Skin irritation judgment result> Prescription Number of testers Judgment result - ≠ + ++ +++ Example 19 20.0 20.0 0 0 0 0 Example 20 20.0 20.0 0 0 0 0 Comparative Example 37 20.0 19.0 0 One 0 0 Comparative Example 38 20.0 19.0 0 One 0 0 Comparative Example 39 20.0 18.0 One One 0 0 Comparative Example 40 20.0 18.0 One One 0 0

Looking at the results in Table 20 above, it can be seen that when comparing the hair dye composition of the Example and Comparative Example of the present composition patent, Comparative Examples 37 to 40 have more irritation than Examples 19 to 20.

That is, in each of Comparative Examples 37 to 40, it can be seen that among the four ingredients, lavender water and lavender oil have no effect of improving stimulation outside the standard range of effects.

<Examples 21 and 22 Preparation of Comparative Examples 41 to 44>

Hair dyes of Examples 21 to 22 and Comparative Examples 41 to 44 were prepared based on the components and contents of the cream-type hair dye prepared in Table 21 below.

In the manufacturing process, after heating the phases A and B in Table 3 to 85℃, homomixing emulsification (RPM: 3,000rpm, Time: 10 minutes, cooling to 50℃) to make a homogeneous phase, and then phase C was added. And homomixing emulsification (RPM: 2,000 rpm, Time: 10 minutes degassing and cooling to complete 30°C).

<Gel type (shampoo type) hair dye manufacturing> Ingredient/content (%) Classification (top) Example Comparative example 21 22 41 42 43 44 Purified water A phase Balance Balance Balance Balance Balance Balance Propylene glycol 5.0 5.0 5.0 5.0 5.0 5.0 Sodium laureth sulfate 5.0 5.0 5.0 5.0 5.0 5.0 Disodium EDTA 0.1 0.1 0.1 0.1 0.1 0.1 Sodium lauryl sulfate 5.0 5.0 5.0 5.0 5.0 5.0 Ethylhexyloctanoate B phase 1.7 1.7 1.7 1.7 1.7 1.7 Ethylhexylstearate 5.0 5.0 5.0 5.0 5.0 5.0 Cocamide D.A. 7.0 7.0 7.0 7.0 7.0 7.0 Oleic Acid 7.0 7.0 7.0 7.0 7.0 7.0 Steatrimonium chloride 3.5 3.5 3.5 3.5 3.5 3.5 Stearyl alcohol 1.0 1.0 1.0 1.0 1.0 1.0 Cetyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 Lauryl alcohol 4.0 4.0 4.0 4.0 4.0 4.0 p-aminophenol C phase 0.35 0.35 0.35 0.35 0.35 0.35 p-phenylenediamine 2.1 2.1 2.1 2.1 2.1 2.1 o-aminophenol 0.05 0.05 0.05 0.05 0.05 0.05 m-aminophenol 0.6 0.6 0.6 0.6 0.6 0.6 Ammonium thioglyconate 0.5 0.5 0.5 0.5 0.5 0.5 Sodium astorbate 0.3 0.3 0.3 0.3 0.3 0.3 Dry sodium sulfite 0.9 0.9 0.9 0.9 0.9 0.9 Monoethanolamine 2.8 2.8 2.8 2.8 2.8 2.8 ammonia 3.0 3.0 3.0 3.0 3.0 3.0 Sodium chloride 0.5 0.5 0.5 0.5 0.5 0.5 Resorcin 0.8 0.8 0.8 0.8 0.8 0.8 Lavender water 5.0 5.0 0.5 0.6 3.0 4.0 Lemongrass extract 2.0 2.0 2.0 3.0 0.4 0.4 Lemon Balm Leaf Extract 2.0 2.0 2.0 3.0 0.4 0.4 Lavender oil 0.6 0.7 0.09 0.09 0.6 0.9 Sodium Thioglyconate 12.0 12.0 12.0 12.0 12.0 12.0 Hydrolyzed Collagen 1.0 1.0 1.0 1.0 1.0 1.0

<Experimental Example 11> Skin irritation test

The hair dyes of Examples 21 to 22 and Comparative Examples 41 to 44 were subjected to a skin irritation experiment in the same manner as in Experimental Example 1, and the results are shown in Table 22 below.

<Skin irritation judgment result> Prescription Number of testers Judgment result - ≠ + ++ +++ Example 21 20.0 20.0 0 0 0 0 Example 22 20.0 20.0 0 0 0 0 Comparative Example 41 20.0 18.0 One One 0 0 Comparative Example 42 20.0 19.0 0 One 0 0 Comparative Example 43 20.0 18.0 One One 0 0 Comparative Example 44 20.0 19.0 One 0 0 0

Looking at the results in Table 22 above, it can be seen that when comparing the hair dye composition of Example and Comparative Example, Comparative Examples 41 to 44 have more irritation than Examples 21 to 22.

That is, it can be seen that the lemon balm leaf extract and lemongrass extract of the four components of Comparative Examples 41 to 44, respectively, have no effect of improving stimulation outside the standard range of effect.

<Preparation of Examples 9 and 10 and Comparative Examples 17 to 20>

The hair dyes of Examples 23 to 24 and Comparative Examples 45 to 48 were prepared based on the ingredients and contents of the gel type (shampoo type) hair dye prepared in Table 23 below.

In the manufacturing process, after heating the phases A and B in Table 23 to 85°C, homomixing emulsification (RPM: 3,000 rpm, Time: 10 minutes, cooling to 50°C) to make a homogeneous phase, and then add phase C. It was prepared by homomixing emulsification (RPM: 2,000 rpm, Time: 10 minutes degassing and cooling to complete at 30°C).

<Manufacture of gel type (shampoo type) hair dye> Ingredient/content (%) Classification (top) Example Comparative example 23 24 45 46 47 48 Purified water A phase Balance Balance Balance Balance Balance Balance Propylene glycol 1.0 1.0 1.0 1.0 1.0 1.0 Sodium laureth sulfate 1.0 1.0 1.0 1.0 1.0 1.0 Disodium EDTA 0.1 0.1 0.1 0.1 0.1 0.1 Sodium lauryl sulfate 1.0 1.0 1.0 1.0 1.0 1.0 Ethylhexyloctanoate B phase 1.7 1.7 1.7 1.7 1.7 1.7 Ethylhexylstearate 1.0 1.0 1.0 1.0 1.0 1.0 Cocamide D.A. 1.0 1.0 1.0 1.0 1.0 1.0 Oleic Acid 1.0 1.0 1.0 1.0 1.0 1.0 Steatrimonium chloride 1.5 1.5 1.5 1.5 1.5 1.5 Stearyl alcohol 1.0 1.0 1.0 1.0 1.0 1.0 Cetyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 Lauryl alcohol 1.0 1.0 1.0 1.0 1.0 1.0 p-aminophenol C phase 0.35 0.35 0.35 0.35 0.35 0.35 p-phenylenediamine 2.1 2.1 2.1 2.1 2.1 2.1 o-aminophenol 0.05 0.05 0.05 0.05 0.05 0.05 m-aminophenol 0.6 0.6 0.6 0.6 0.6 0.6 Ammonium thioglyconate 0.5 0.5 0.5 0.5 0.5 0.5 Sodium astorbate 0.3 0.3 0.3 0.3 0.3 0.3 Dry sodium sulfite 0.9 0.9 0.9 0.9 0.9 0.9 Monoethanolamine 2.8 2.8 2.8 2.8 2.8 2.8 ammonia 3.0 3.0 3.0 3.0 3.0 3.0 Sodium chloride 0.5 0.5 0.5 0.5 0.5 0.5 Resorcin 0.8 0.8 0.8 0.8 0.8 0.8 Lavender water 10.0 20.0 51.0 40.0 30.0 20.0 Lemongrass extract 3.0 3.0 4.0 6.0 3.0 3.5 Lemon Balm Leaf Extract 3.0 3.0 4.0 3.0 6.0 4.5 Lavender oil 8.0 7.0 8.0 6.0 8.0 8.1 Sodium Thioglyconate 4 4 4 4 4 4 Hydrolyzed Collagen 1.0 1.0 1.0 1.0 1.0 1.0

<Experimental Example 12> Skin irritation test

The hair dyes of Examples 23 to 24 and Comparative Examples 45 to 48 were subjected to a skin irritation experiment in the same manner as in Experimental Example 1, and the results are shown in Table 24 below.

<Skin irritation judgment result> Prescription Number of testers Judgment result - ≠ + ++ +++ Example 23 20.0 20.0 0 0 0 0 Example 24 20.0 20.0 0 0 0 0 Comparative Example 45 20.0 18.0 One One 0 0 Comparative Example 46 20.0 19.0 One 0 0 0 Comparative Example 47 20.0 18.0 One One 0 0 Comparative Example 48 20.0 19.0 One 0 0 0

Looking at the results in Table 24 above, it can be seen that Comparative Examples 45 to 48 have more irritation than Examples 23 to 24 when comparing the hair dye composition of Examples and Comparative Examples.

That is, each of the four components of Comparative Examples 45 to 48 is lavender water 51: lemon grass extract 6: lemon balm leaf extract 6: lavender oil 8.1, which is a higher effective content. I can.

<Examples 25 and 26 Preparation of Comparative Examples 49 to 52>

The hair dyes of Examples 25 to 26 and Comparative Examples 49 to 52 were prepared based on the ingredients and contents of the gel type (shampoo type) hair dye prepared in Table 25 below.

In the manufacturing process, after heating the phases A and B in Table 3 to 85℃, homomixing emulsification (RPM: 3,000rpm, Time: 10 minutes, cooling to 50℃) to make a homogeneous phase, and then phase C was added. And homomixing emulsification (RPM: 2,000 rpm, Time: 10 minutes degassing and cooling to complete 30°C).

<Manufacture of gel type (shampoo type) hair dye> Ingredient/content (%) Classification (top) Example Comparative example 25 26 49 50 51 52 Purified water A phase Balance Balance Balance Balance Balance Balance Propylene glycol 1.0 1.0 1.0 1.0 1.0 1.0 Sodium laureth sulfate 1.0 1.0 1.0 1.0 1.0 1.0 Disodium EDTA 0.1 0.1 0.1 0.1 0.1 0.1 Sodium lauryl sulfate 1.0 1.0 1.0 1.0 1.0 1.0 Ethylhexyloctanoate B phase 1.7 1.7 1.7 1.7 1.7 1.7 Ethylhexylstearate 1.0 1.0 1.0 1.0 1.0 1.0 Cocamide D.A. 1.0 1.0 1.0 1.0 1.0 1.0 Oleic Acid 1.0 1.0 1.0 1.0 1.0 1.0 Steatrimonium chloride 1.5 1.5 1.5 1.5 1.5 1.5 Stearyl alcohol 1.0 1.0 1.0 1.0 1.0 1.0 Cetyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 Lauryl alcohol 1.0 1.0 1.0 1.0 1.0 1.0 p-aminophenol C phase 0.35 0.35 0.35 0.35 0.35 0.35 p-phenylenediamine 2.1 2.1 2.1 2.1 2.1 2.1 o-aminophenol 0.05 0.05 0.05 0.05 0.05 0.05 m-aminophenol 0.6 0.6 0.6 0.6 0.6 0.6 Ammonium thioglyconate 0.5 0.5 0.5 0.5 0.5 0.5 Sodium astorbate 0.3 0.3 0.3 0.3 0.3 0.3 Dry sodium sulfite 0.9 0.9 0.9 0.9 0.9 0.9 Monoethanolamine 2.8 2.8 2.8 2.8 2.8 2.8 ammonia 3.0 3.0 3.0 3.0 3.0 3.0 Sodium chloride 0.5 0.5 0.5 0.5 0.5 0.5 Resorcin 0.8 0.8 0.8 0.8 0.8 0.8 Lavender water 30.0 40.0 50.5 10.5 50.1 50.0 Lemongrass extract 4.0 2.0 5.1 4.5 5.0 5.1 Lemon Balm Leaf Extract 4.0 2.0 3.0 5.1 5.1 5.0 Lavender oil 5.0 4.0 8.0 8.1 8.0 8.1 Sodium Thioglyconate 4 4 4 4 4 4 Hydrolyzed Collagen 1.0 1.0 1.0 1.0 1.0 1.0

<Experimental Example 13> Skin irritation test

The hair dyes of Examples 25 to 26 and Comparative Examples 49 to 52 were subjected to a skin irritation experiment in the same manner as in Experimental Example 1, and the results are shown in Table 27 below.

<Skin irritation judgment result> Prescription Number of testers Judgment result - ≠ + ++ +++ Example 25 20.0 20.0 0 0 0 0 Example 26 20.0 20.0 0 0 0 0 Comparative Example 49 20.0 18.0 One One 0 0 Comparative Example 50 20.0 18.0 One One 0 0 Comparative Example 51 20.0 18.0 One One 0 0 Comparative Example 52 20.0 19.0 One 0 0 0

Looking at the results of the table above, it can be seen that when comparing the hair dye composition of the Example and Comparative Example, Comparative Examples 49 to 52 have more irritation than Examples 25 to 26.

In other words, it can be seen that two or more of the four components of Comparative Examples 49 to 52 have a higher content, respectively, out of the effect reference range, and there is no effect of improving stimulation.

<Examples 27 and 28 Preparation of Comparative Examples 53 to 56>

The hair dyes of Examples 27-28 and Comparative Examples 53-56 were prepared based on the ingredients and contents of the gel-type (shampoo-type) hair dye prepared in Table 27 below.

In the manufacturing process, after heating the phases A and B in Table 3 to 85℃, homomixing emulsification (RPM: 3,000rpm, Time: 10 minutes, cooling to 50℃) to make a homogeneous phase, and then phase C was added. And homomixing emulsification (RPM: 2,000 rpm, Time: 10 minutes degassing and cooling to complete 30°C).

<Manufacture of gel type (shampoo type) hair dye> Ingredient/content (%) Classification (top) Example Comparative example 27 28 53 54 55 56 Purified water A phase Balance Balance Balance Balance Balance Balance Propylene glycol 1.0 1.0 1.0 1.0 1.0 1.0 Sodium laureth sulfate 1.0 1.0 1.0 1.0 1.0 1.0 Disodium EDTA 0.1 0.1 0.1 0.1 0.1 0.1 Sodium lauryl sulfate 1.0 1.0 1.0 1.0 1.0 1.0 Ethylhexyloctanoate B phase 1.7 1.7 1.7 1.7 1.7 1.7 Ethylhexylstearate 1.0 1.0 1.0 1.0 1.0 1.0 Cocamide D.A. 1.0 1.0 1.0 1.0 1.0 1.0 Oleic Acid 1.0 1.0 1.0 1.0 1.0 1.0 Steatrimonium chloride 1.5 1.5 1.5 1.5 1.5 1.5 Stearyl alcohol 1.0 1.0 1.0 1.0 1.0 1.0 Cetyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 Lauryl alcohol 1.0 1.0 1.0 1.0 1.0 1.0 p-aminophenol C phase 0.35 0.35 0.35 0.35 0.35 0.35 p-phenylenediamine 2.1 2.1 2.1 2.1 2.1 2.1 o-aminophenol 0.05 0.05 0.05 0.05 0.05 0.05 m-aminophenol 0.6 0.6 0.6 0.6 0.6 0.6 Ammonium thioglyconate 0.5 0.5 0.5 0.5 0.5 0.5 Sodium astorbate 0.3 0.3 0.3 0.3 0.3 0.3 Dry sodium sulfite 0.9 0.9 0.9 0.9 0.9 0.9 Monoethanolamine 2.8 2.8 2.8 2.8 2.8 2.8 ammonia 3.0 3.0 3.0 3.0 3.0 3.0 Sodium chloride 0.5 0.5 0.5 0.5 0.5 0.5 Resorcin 0.8 0.8 0.8 0.8 0.8 0.8 Lavender water 50.0 50.0 50.1 20.0 50.1 25.0 Lemongrass extract 2.0 3.0 1.5 5.1 5.1 5.0 Lemon Balm Leaf Extract 3.0 2.0 2.0 5.1 5.0 5.1 Lavender oil 5.0 8.0 8.1 8.0 8.0 8.1 Sodium Thioglyconate 4 4 4 4 4 4 Hydrolyzed Collagen 1.0 1.0 1.0 1.0 1.0 1.0

<Experimental Example 14> Skin irritation test

The hair dyes of Examples 27 to 28 and Comparative Examples 53 to 56 were subjected to a skin irritation experiment in the same manner as in Experimental Example 1, and the results are shown in Table 28 below.

<Skin irritation judgment result> Prescription Number of testers Judgment result - ≠ + ++ +++ Example 27 20.0 20.0 0 0 0 0 Example 28 20.0 20.0 0 0 0 0 Comparative Example 53 20.0 18.0 One One 0 0 Comparative Example 54 20.0 18.0 One One 0 0 Comparative Example 55 20.0 18.0 One One 0 0 Comparative Example 56 20.0 18.0 One One 0 0

Looking at the results in Table 28 above, it can be seen that when comparing the hair dye composition of Example and Comparative Example, Comparative Examples 53 to 56 have more irritation than Examples 27 to 28.

That is, out of the four ingredients of Comparative Examples 53 to 56, two or more of lavender water, lavender oil, lemon balm leaf extract, and lemongrass extract each had a higher content, respectively, out of the reference range of the effect, and there was no effect of improving stimulation.

<Experimental Example 15> Deodorant effect evaluation test

In order to evaluate the degree of deodorization, 5 people as a group based on people aged 30 to 60 years without skin disease, applying the examples and comparative examples of the cream hair dye above were carried out for 1 month, and the deodorizing effect was as follows. It evaluated according to the evaluation criteria, and the results are shown in Table 29.

<Deodorant evaluation criteria>

(1: Very unsuitable, 2: Unsuitable, 3: Good, 4: Excellent, 5: Very good)

<Deodorant effect result> division Example Comparative example One 2 One 2 3 4 Deodorization evaluation 4.5 4.3 2.8 3.6 3.4 3.1 division Example Comparative example 3 4 5 6 7 8 Deodorization evaluation 4.3 4.3 3.1 3.3 3.2 3.2 division Example Comparative example 5 6 9 10 11 12 Deodorization evaluation 4.6 4.5 3.0 3.1 3.2 3.0 division Example Comparative example 7 8 13 14 15 16 Deodorization evaluation 4.5 4.4 3.3 3.3 3.4 2.7

As shown in Table 29 above, it was found that the hair-dye composition outside the effective range of the extract, as in Comparative Example, had less deodorizing effect than the examples in which the extract was within the effective range.

<Experimental Example 16> Deodorant effect evaluation test

In order to evaluate the degree of deodorization, 5 people as a group based on people aged 30 to 60 years without skin disease and applying the above gel type (shampoo type) hair dye examples and comparative examples were conducted for 1 month. The deodorizing effect was evaluated according to the evaluation criteria as follows, and the results are shown in Table 30.

<Deodorant evaluation criteria>

(1: Very unsuitable, 2: Unsuitable, 3: Good, 4: Excellent, 5: Very good)

<Deodorant effect result> division Example Comparative example 15 16 29 30 31 32 Deodorization evaluation 4.3 4.1 2.4 3.4 3.3 32.7 division Example Comparative example 17 18 33 34 35 36 Deodorization evaluation 4.4 4.2 3.2 3.3 2.8 3.3 division Example Comparative example 19 20 37 38 39 40 Deodorization evaluation 4.4 4.5 3.1 2.9 2.7 3.0 division Example Comparative example 21 22 41 42 43 44 Deodorization evaluation 4.3 4.5 2.8 3.1 3.2 2.9

As shown in Table 30 above, it was found that, as in Comparative Examples, the hair-dye composition outside the effective range of the extract had less deodorizing effect than the examples in which the extract was within the effective range.

<Experimental Example 17> Antibacterial experiment

The antibacterial effect against E. coli and Pseudomonas aeruginosa was performed for the cream hair dye consisting of the following composition, that is, Examples 9 and 10 and Comparative Examples 17 and 18.

Escherichia coli and Pseudomonas aeruginosa were cultured in a liquid medium (Brain heart infusion broth) and enriched and then tested.

Ingredient/content (%) Classification (top) Comparative example
17(control) Example 9
(E.coli) Comparative Example 18
(control) Example 10 (P.aeruginosa) Purified water A phase Balance Balance Balance Balance Allantoin 0.5 0.5 0.5 0.5 glycerin 0.1 0.1 0.1 0.1 Polyoxyethylene lauryl ether B phase 2.5 2.5 2.5 2.5 Polyoxyethylene oleyl ether 1.5 1.5 1.5 1.5 Cetostearyl alcohol 1.5 1.5 1.5 1.5 Cetyl alcohol 1.5 1.5 1.5 1.5 p-aminophenol 1.0 1.0 1.0 1.0 Resorcinol 1.0 1.0 1.0 1.0 Monoethanolamine 0.6 0.6 0.6 0.6 ammonia 3.5 3.5 3.5 3.5 Hydrolyzed Collagen C phase 1.0 1.0 1.0 1.0 Lavender water 51.0 10.0 40.0 20.0 Lemongrass extract 4.0 3.0 6.0 3.0 Lemon Balm Leaf Extract 4.0 3.0 3.0 3.0 Lavender oil 8.0 8.0 6.0 7.0 Hydrogen peroxide (30%) 17.0 17.0 17.0 17.0 Phosphoric acid 0.1 0.1 0.1 0.1

In addition, the culture solution of E. coli and Pseudomonas aeruginosa was inoculated with a sterile cotton swab on the prepared TSA (Trytic Soy Agar) plate medium. Inoculation was carried out on PCA agar plate (Ec, Pa (1.0x108 CFU/ml)), dried for 5 minutes, placed 35 mg of Examples and Comparative Examples on a sterilized disc (8.0mm), placed in the center of the medium, and then at 35°C After incubation for 24 hours, the width of the inhibition zone was measured according to the following equation, and are shown in Table 32 and Figures 1 and 2 below.

<Antibacterial calculation formula>

W=(T-D)/2

T: total diameter of sample and microbial barrier (mm)

D: sample diameter (mm)

W: width of low-lying zone (mm)

Sample classification T (unit: mm) D (unit: mm) Lowland width (w) (unit: mm Comparative Example 17 (Control) - - - Example 9 (E.coli) 12.6 7 2.8 Comparative Example 18 (Control) - - - Example 10 (P.aeruginosa) 10.8 7 1.9

Experimental Results As a result of measuring the size of the inhibition zone for the samples of Examples 9 and 10, a low-lying zone of 2.8 mm was formed for E.Coli, and a low-lying zone of 1.9 mm was formed for P.aeruginosa as shown in Table 31. The compositions of Examples 9 and 10 had excellent antibacterial effects against Escherichia coli and Pseudomonas aeruginosa, respectively, and it was found that they had a deodorant effect by antibacterial (see Figs. 1 and 2).

<Experimental Example 18> Antibacterial experiment

The antibacterial effect against E. coli and Pseudomonas aeruginosa was performed for the gel-type hair dyes Examples 15 and 16 and Comparative Examples 29 and 30 having the following composition.

Escherichia coli and Pseudomonas aeruginosa were cultured in a liquid medium (Brain heart infusion broth) and enriched and then tested.

<Manufacture of gel type (shampoo type) hair dye> Ingredient/content (%) Classification (top) Example Comparative example 15 16 29 30 Purified water A phase Balance Balance Balance Balance Propylene glycol 5.0 5.0 5.0 5.0 Sodium laureth sulfate 5.0 5.0 5.0 5.0 Disodium EDTA 0.1 0.1 0.1 0.1 Sodium lauryl sulfate 5.0 5.0 5.0 5.0 Ethylhexyloctanoate B phase 1.7 1.7 1.7 1.7 Ethylhexylstearate 5.0 5.0 5.0 5.0 Cocamide D.A. 7.0 7.0 7.0 7.0 Oleic Acid 7.0 7.0 7.0 7.0 Steatrimonium chloride 3.5 3.5 3.5 3.5 Stearyl alcohol 1.0 1.0 1.0 1.0 Cetyl alcohol 3.0 3.0 3.0 3.0 Lauryl alcohol 1.0 1.0 1.0 1.0 p-aminophenol C phase 0.35 0.35 0.35 0.35 p-phenylenediamine 2.1 2.1 2.1 2.1 o-aminophenol 0.05 0.05 0.05 0.05 m-aminophenol 0.6 0.6 0.6 0.6 Ammonium thioglyconate 0.5 0.5 0.5 0.5 Sodium astorbate 0.3 0.3 0.3 0.3 Dry sodium sulfite 0.9 0.9 0.9 0.9 Monoethanolamine 2.8 2.8 2.8 2.8 ammonia 3.0 3.0 3.0 3.0 Sodium chloride 0.5 0.5 0.5 0.5 Resorcin 0.8 0.8 0.8 0.8 Lavender water 1.0 1.0 - 1.0 Lemongrass extract 0.5 0.5 0.5 - Lemon Balm Leaf Extract 0.5 0.5 0.5 0.5 Lavender oil 0.1 0.2 0.1 0.1 Sodium Thioglyconate 15.0 15.0 15.0 15.0 Hydrolyzed Collagen 1.0 1.0 1.0 1.0

In addition, the culture solution of E. coli and Pseudomonas aeruginosa was inoculated with a sterile cotton swab on the prepared TSA (Trytic Soy Agar) plate medium. Inoculation was carried out on PCA agar plate (Ec, Pa (1.0x108 CFU/ml)), dried for 5 minutes, and then 35 mg of Examples and Comparative Examples were placed on a sterilized disc (8.0mm), placed in the center of the medium, and then at 35°C. After incubation for 24 hours, the width of the inhibition zone was measured according to the following formula and shown in Table 34 below.

<Antibacterial calculation formula>

W=(T-D)/2

T: total diameter of sample and microbial barrier (mm)

D: sample diameter (mm)

W: width of low-lying zone (mm)

Sample classification T (unit: mm) D (unit: mm) Lowland width (w) (unit: mm Comparative Example 29 (Control) - - - Example 15 (E.coli) 11.8 7 2.4 Comparative Example 30 (Control) - - - Example 16 (P.aeruginosa) 12.4 7 2.7

Experimental Results As a result of measuring the size of the inhibition zone for the samples of Examples 15 and 16, a low-lying zone of 2.4 mm was formed for E.Coli, and a low-lying zone of 2.7 mm was formed for P.aeruginosa as shown in Table 34. The compositions of Examples 15 and 16 had excellent antibacterial effects against Escherichia coli and Pseudomonas aeruginosa, respectively, and it was found that they had a deodorant effect by antibacterial.

Claims (9)

In the hair dye composition,
Lavender water 1 to 50% by weight, lemon balm leaf extract 0.5 to 5.0% by weight, lemongrass extract 0.5 to 5.0% by weight, lavender oil, characterized in that it contains 0.1 to 8.0% by weight as an active ingredient,
Hair-dye composition having antibacterial, skin irritation relief, deodorant and ammonia odor removal effect.
delete delete The method of claim 1,
The lavender water, lemon balm leaf extract, and lemongrass extract are obtained by hot water extraction or organic solvent extraction method,
The lavender oil is characterized in that obtained by heating and distilling after mixing water,
Hair-dye composition having antibacterial, skin irritation relief, deodorization and ammonia odor removal.
delete The method of claim 1,
The active ingredient is characterized in that it contains 2.1 to 68.0% by weight of the total weight of the hair dye composition,
Hair-dye composition having antibacterial, skin irritation relief, deodorant and ammonia odor removal effect.
The method of claim 1,
The hair-dye composition is characterized in that the formulation is a cream type,
Hair-dye composition having antibacterial, skin irritation relief, deodorant and ammonia odor removal effect.
The method of claim 1,
The hair-dye composition is characterized in that the formulation is a gel type,
Hair-dye composition having antibacterial, skin irritation relief, deodorant and ammonia odor removal effect.
In the method for producing a hair dye composition,
Obtaining lavender water, lemon balm leaf extract, and lemon grass extract through hot water extraction or organic solvent extraction using lavender, lemon balm leaves, and lemon grass as raw materials;
Mixing lavender as a raw material with water, heating and distilling to obtain lavender oil;
The lavender water 1 to 50% by weight, lemon balm leaf extract 0.5 to 5.0% by weight, lemongrass extract 0.5 to 5.0% by weight, lavender oil is 0.1 to 8.0% by weight of a hair dye composition raw material, comprising;
A method of preparing a hair-dye composition having antibacterial, skin irritation relief, deodorization and ammonia odor removal effect.

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