CN104254318A

CN104254318A - Cosmetic composition containing the slime of snails fed with red ginseng and method for manufacturing same

Info

Publication number: CN104254318A
Application number: CN201280065814.2A
Authority: CN
Inventors: 朴成敏; 朴成鲁; 尹美英; 朴志泳
Original assignee: COSEEDBIOPARM Co Ltd
Current assignee: COSEEDBIOPARM Co Ltd
Priority date: 2011-12-13
Filing date: 2012-12-13
Publication date: 2014-12-31
Also published as: PH12014501342B1; KR101208012B1; WO2013089449A1; PH12014501342A1

Abstract

The present invention relates to a cosmetic composition containing the snail derived from snails fed with red ginseng, which provides effects that are superior to those of slime from snails raised using typical methods in terms of wound healing, skin recovery, and alleviating atopy and skin irritation.

Description

The cosmetic composition of the Limax mucus of feeding containing useful Radix Ginseng Rubra and manufacture method thereof

Technical field

The present invention relates to the cosmetic composition containing Limax mucus.More specifically, the present invention relates to the cosmetic composition of the Limax mucus containing the Limax being derived from Radix Ginseng Rubra of feeding.

Background technology

Generally speaking, the healing of the wound caused due to the damage to skin relates to quite complicated step, comprises inflammation, cell proliferation, cell migration, vascular proliferation and cellular matrix formation etc.These steps are carried out successively, but overlapping to a certain extent, do not distinguish clearly therebetween.Healing Rate and level depend on tissue and wound healing method and change.In other words, there is various types of skin trauma, and each wound have the anatomy that is mutually related, physiology, pathology and microbiological factor according to its type.Relate to all of these factors taken together in wound healing, but wound healing method is based on following.

According to various method, wound healing is classified.Wound healing is divided into four-stage by Johnston: the removal of inflammation, foreign body, reparation and maturation.It should be noted that the feature of wound healing process is that these stages are successively.

Generally speaking, when tissue sustains damage, celliferous structure, physiology and chemistry change, and impaired or defective position is thoroughly regenerated or heal, while scar.Wound shrinkage is the key factor in defective skin wound healing process, and it refers to the phenomenon that wherein rejected region is reduced by the epitheliosis of wound edge and the contraction of granulation tissue.The contractile mechanism of granulation tissue is clear unknown yet.The theory causing granulation tissue to shrink by the contractility of muscle fibroblast (myofibroblast) is considered to the most convictive.

Wound healing process repairs the biological response of damaged tissues, and the fragment of tissue of hemopexis when comprising hemorrhage, Fibrin exudation, destruction is engulfed by cytophagous mobilization, the propagation of promoting epidermization, granulation tissue, new blood capillary and fiber fibroblast and by the fibrosis of being synthesized by the fibroblast bred and the Collagen fiber deposition of secretion causes.Formation or the epithelial covering of the research recently relating to wound healing and the formation of finding out granulation tissue and collagen are relevant, and described granulation tissue is the filling tissue of defective wound.

Meanwhile, horny layer of epidermis is present in outmost skin, and provides by moisture outside suppression skin and electrolytical loss the condition preventing xerosis cutis from also can realize normal skin biochemical mechanism.In addition, horny layer of epidermis is being protected the injury of body-defence external physical or chemicals or is preventing antibacterial and virus etc. from invading skin important role.

When Dermal exposure is in time causing skin irritant material as sodium lauryl sulphate, dimethyl sulfoxine, Oleum Tiglii or sodium hydroxide, the impaired and transepidermal water loss as the index of cuticle loss of horny layer increases.

Therefore, carried out the opposing of protection skin is caused to skin irritant material and alleviates the research stimulated.The known oil in water emulsion protection skin containing glycerol from the skin irritation caused by sodium lauryl sulphate or dimethyl sulfoxine, and it is reported that protectiveness cream has the skin irritant effect of relieve chronic.

Meanwhile, Limax belongs to Gastropoda, and there will be a known about 20 in the world, 000 kind of Limax.Find Limax in such as Korea S, Japan and the Chinese zone of constant temperature regional and torrid areas.Limax population is isolated regional due to Hypomobility, and therefore has many subspecies.The example of Limax species comprises the Africangiant snail (Achatina achatina) with large scale (the shell height of 100mm); There is the rib watt Lou Limax (Valononia costata) of the shell height of about 1mm; And live in the large Limax (Limax maximus) of Brazil, it has the ovum length of 27mm and the ovum diameter of about 12mm.

The composition forming Limax mucus is mucin, and to form mucinous composition be chondroitin sulfate.The Major Nutrient component of Limax mucus is protein (54.29%), lipid (4.18%), saccharide (30.45%) and ash (4.07%) etc.Allantoin as Limax mucus key component has the effect of skin smooth and pore contraction, and glycolic effectively can prevent skin problem, and known collagen and elastin laminin keep skin elasticity.

As the prior art relevant to the purposes of Limax in cosmetics, the Korean Patent Publication No. 10-1995-0002741 (February 16 nineteen ninety-five is open) that name is called " Method for preparing cosmetic containing mucin extracted from snails " discloses and is immersed in hot salt brine the Limax of washing with water purification thoroughly to remove the impurity existed in Limax; Liquid phase mucin is collected from Limax; The mucin that heating is collected with evaporation moisture wherein, thus extracts pure mucin; And in the process of mixed base raw material, add extracted mucin and mucin is mixed with basic material thus prepares cosmetics.

Meanwhile, Radix Ginseng is the herbaceos perennial belonging to Araliaceae.The root of Radix Ginseng in the traditional Chinese medical science, be called " Radix Ginseng (ginseng radix) " and known Radix Ginseng has the effect such as nourishing, promote energy, hemopoietic, insulation, strong stomach, fatigue recovery, mental toughness and calmness.The main active of Radix Ginseng comprises saponin, sapogenin, polyacetylene, pyrazines derivatives and maltol etc., and the saponin of ginsenoside is divided into protopanoxadiol, Protopanaxatriol and oleanane (oleanonane) saponin according to the character of chemical constitution.Up to now, as protopanoxadiol, Protopanaxatriol and oleanane saponin, abstraction and purification 19 classes, 10 classes and 1 compounds are distinguished.Known protopanoxadiol and Protopanaxatriol have different pharmacological actions in vivo.

Saponin contained in ginsenoside is known as nontoxic, be different from the saponin of other plant, and 0.001% or lower time, do not represent haemolysis, and it is reported that it suppresses central nervous system, promotes protein synthesis, promotes corticosteroid secretion and strengthen immunologic function etc.

Meanwhile, Radix Ginseng Rubra refers to by not removing the peel Radix Ginseng decatize and dry and finished Radix Ginseng that is that produce repeatedly by fresh.Vision difference between Radix Ginseng Rubra and fresh ginseng is that Radix Ginseng Rubra is fawn or light reddish brown color, and has the bioactive substance of the unusual high-load of every g unit due to the moisture evaporation between dry period.During the decatize of Radix Ginseng Rubra, saponin glycocide (saponin glycoside) is converted into saponin, make saponin concentrations increase and the quantity of saponin kind is increased to 32 kinds.

Summary of the invention

Technical problem

Therefore, complete the present invention in view of above problem, and the object of this invention is to provide the cosmetic composition utilizing Radix Ginseng Rubra and Limax mucus with excellent effect, it is applicable to cosmetics for skin and is derived from harmless natural materials.

Technical scheme

According to an aspect of the present invention, the cosmetic composition being contained Limax mucus by providing package can realize above and other object, and described Limax mucus obtains from the Limax fed containing the meals of Red Ginseng.

Limax in the present invention is not specifically limited.Such as, Limax can be Africangiant snail (Achatina fulica) or housing helix (Helix pomatia).

Meanwhile, in cosmetic composition of the present invention, the amount of Red Ginseng preferably with 0.1 % by weight ~ 30 % by weight in meals exists.

Meanwhile, in cosmetic composition of the present invention, Limax mucus preferably comprises chondroitin sulfate, allantoin and glycolic.

Meanwhile, in cosmetic composition of the present invention, relative to the gross weight of cosmetic composition, Limax mucus preferably exists with the amount of 0.001 % by weight ~ 30.0 % by weight.

Meanwhile, cosmetic composition of the present invention is preferably used for alleviating stimulates.

Meanwhile, cosmetic composition of the present invention is preferably used for atopy alleviation.

Meanwhile, cosmetic composition of the present invention is preferably used for skin moisture-keeping (skin moisturizing).

Simultaneously, cosmetic composition of the present invention preferably has the preparation being selected from skin protection cosmetics preparation and cosmetics (make-up cosmetic formulation) preparation, described skin protection cosmetics preparation is by toner (toner), gel, water-soluble liquid, cream, essence (essences), oil-in-water (O/W) type and Water-In-Oil (W/O) type Emulsion and unguentum composition, and described cosmetics preparation is by adornment (makeup base) at the bottom of oil-in-water (O/W) type and Water-In-Oil (W/O) type, foundation cream, hide flaw agent (skin cover), lip pomade, lip gloss (lip glosses), face powder, dual-purpose muffin, eye shadow, cheek coloured silk and eyebrow pencil composition.

Hereinafter, many aspects of the present invention will be described in detail.

The present inventor have studied the suitability of various natural materials to cosmetics.Result is; the present inventor have selected Limax mucus; and find that the mucus obtained from the Limax of Radix Ginseng Rubra of feeding has better healing, skin regeneration and skin irritation remission effect than the mucus of the Limax grown by usual method; and estimate that it represents excellent cosmetic result thus, thus complete the present invention.

Limax mucus as the key component of cosmetic composition of the present invention is from feeding the mucus having the Limax of Radix Ginseng Rubra to obtain.The mucus obtained from the Limax fed with Radix Ginseng Rubra is more effective than conventional Limax mucus in cell activation, skin moisture-keeping and atopical skin regeneration, therefore represents excellent wound healing and skin resurfacing effect.

Even more effective than conventional Limax mucus in wound healing, skin regeneration and skin irritation alleviation according to the Limax mucus that the present invention obtains from the Limax of Radix Ginseng Rubra of feeding, because described Limax mucus is rich in chondroitin sulfate, allantoin, glycolic etc. very much.

Meanwhile, according to the present invention, feed Limax with Limax meals, described Limax meals contain 0.1 % by weight ~ 30 % by weight, preferably the Radix Ginseng Rubra of amount of 7 % by weight ~ 10 % by weight.When Radix Ginseng Rubra content is lower than 0.1% weight, Limax is not fed by Radix Ginseng Rubra abundance, and when Radix Ginseng Rubra content is higher than 30% weight, because the raising of effect is lower compared with content, therefore business efficiency is low.

Meanwhile, in cosmetic composition according to the present invention, the Limax mucus amount obtained from the Limax of Radix Ginseng Rubra of feeding is not limited to certain scope, and is preferably 0.001 % by weight ~ 30 % by weight relative to the gross weight of cosmetic composition.When cosmetic composition contains the Limax mucus of defined amount, the effect of the Limax mucus obtained from the Limax of Radix Ginseng Rubra of feeding fully is manifested, and does not damage the quality of cosmetic composition simultaneously.

Meanwhile, except the Limax mucus obtained from the Limax of Radix Ginseng Rubra of feeding, can other component promoting main efficacy results be contained, as long as the main efficacy results that the present invention will realize is not compromised according to cosmetic composition of the present invention.

Simultaneously, according to the adjuvant that compositions of the present invention can be commonly used containing one or more cosmetic fields, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipotropy activator, antiseptic, antioxidant, solvent, aromatic, filler, screening agent (blocks), pigment, odor absorbent and dyestuff.The amount of these adjuvants various is its conventional levels used in the art, and such as being 0.001 % by weight ~ 30 % by weight relative to composition total weight.But in all cases, the kind of adjuvant and the selection of ratio make it not affect the desirable properties of cosmetic composition of the present invention negatively.

Simultaneously, cosmetic composition of the present invention has very strong skin regeneration and wound healing effect due to quite excellent skin moisture-keeping, cell activation and atopy remission effect, and is therefore used to skin regeneration, wound healing, skin irritation alleviation, atopy alleviation and skin moisture-keeping.

Simultaneously, cosmetic composition of the present invention preferably has the preparation being selected from skin protection cosmetics preparation and cosmetics preparation, described skin protection cosmetics preparation is made up of toner, gel, water-soluble liquid, cream, essence, oil-in-water (O/W) type and Water-In-Oil (W/O) type Emulsion and unguentum, and described cosmetics preparation is made up of adornment, foundation cream, the agent of the screening flaw, lip pomade, lip gloss, face powder, dual-purpose muffin, eye shadow, cheek coloured silk and eyebrow pencil at the bottom of oil-in-water (O/W) type and Water-In-Oil (W/O) type.

In addition, cosmetic composition can be used as aerosol by optionally application to skin, can be used as solid form (such as rod type) and uses, and can be used as skin-protection product and/or cosmetics are used.

Beneficial effect

The invention provides the method preparing Limax mucus used for cosmetic, described cosmetics are quite effective in wound healing, skin regeneration and skin irritation alleviation.

In addition, the invention provides cosmetic composition, it is for body harmless, and therefore has excellent stability owing to using the Limax mucus that obtains from the Limax of Radix Ginseng Rubra of feeding.

In addition; the invention provides multi-functional cosmetic composition; it is significantly effective in skin moisture-keeping, cell activation, wound healing and atopy alleviation, and therefore has skin regeneration owing to using the Limax mucus that obtains from the Limax of Radix Ginseng Rubra of feeding and stimulate the effect alleviated.

In addition, the invention provides cosmetic composition, it is significantly effective in skin nursing, and this is owing to strong regeneration effect and the stimulation remission effect owing to using the Limax mucus obtained from the Limax of Radix Ginseng Rubra of feeding.

Accompanying drawing explanation

Above-mentioned and other object, other advantage of characteristic sum of the present invention more clearly will be understood by reference to the accompanying drawings from following detailed description of the invention, wherein:

Fig. 1 is wound figure over time in the experimental rat model being presented at full thickness cutaneous defect.

Detailed description of the invention

Hereinafter, in more detail the present invention is described with reference to following examples.Scope of the present invention is not limited to following examples, and contains the remodeling equivalent in fact to its technical spirit.

[preparation example: prepare Limax mucus from the Limax of Radix Ginseng Rubra of feeding]

Select 5 age Radix Ginseng Rubra as sample and it fully washed and grinds.The Radix Ginseng Rubra of grinding is added into general Limax meals with 10% (w/w) and feeds to Limax.Limax for testing is Africangiant snail or housing helix, select the Limax that only grown for 5 ~ June, and described Limax has 5 ~ 10cm, the preferably size of 6 ~ 8cm.By feeding continuously to the Limax selected 150 ~ 180 days for this reason of Radix Ginseng Rubra meals every day 1 ~ 4 time.

After the Limax of the scheduled time of having fed with the water purification washing Radix Ginseng Rubra of flowing, only select the Limax with similar size (6 ~ 8cm), thoroughly sterilize with the ethanol of 70%, and weigh to obtain the mucus of precise volume subsequently.

After collecting 6 hours mucus from the Limax in black net, Limax of again feeding, and left standstill the convalescent period of 12 hours subsequently, to collect mucus with identical mode above and by freezing for the mucus of collecting.

Be housing helix for the following Limax type comparing preparation example, and in following test, use the mucus produced from housing helix.

[comparing preparation example 1: the acquisition of general Limax mucus]

Obtain Limax mucus in the mode identical with preparation example 1, difference is Radix Ginseng Rubra not to be added into Limax meals.

Following table 1 is presented at preparation example and compares the composition of Limax meals used in preparation example.

Table 1

?	Preparation example	Relatively preparation example
			Testa oryzae	27～30％	37％
Roughage (crude food)	20％	20％
			Calcium carbonate (limestone)	20％	20％
Glycine max (L.) Merr.	23％	23％
			Radix Ginseng Rubra	7～10％	0％
Total content	100％	100％

%: based on weight

[comparing preparation example 2: the acquisition of Radix Ginseng Rubra extract]

Select the 5 age Radix Ginseng Rubra identical with preparation example as sample and it fully washed and grinds.Subsequently, 70% ethanol water of 1,000mL is added to 100g Radix Ginseng Rubra, described solution containing proportional be water and the ethanol of 3:7, then at room temperature leaching (leach out) 5 days.Filter products therefrom, and to obtain dry extract, the extract of drying is dissolved in the 1,3 butylene glycol solution of 30% with the concentration of 1% (w/v) by concentrated under vacuo for filtrate, filtering solution and filtrate is used for test of the present invention.

[comparing preparation example 3: the preparation of the mixture of Limax mucus and Radix Ginseng Rubra extract]

Prepare the mixture of the mucus comparing the Limax of the Radix Ginseng Rubra of not feeding of preparation example 1 and the Radix Ginseng Rubra extract comparing preparation example 2.The Radix Ginseng Rubra of the comparison preparation example 1 of herein adding is respectively 50% (w/w) with the amount of the Radix Ginseng Rubra extract comparing preparation example 2.

[test case 1: the measurement of cytoactive]

In this test case; measure cytoactive in laboratory conditions, to check the Limax mucus obtained from the Limax of the Radix Ginseng Rubra of feeding of preparation example, the Limax mucus comparing preparation example 1, the Radix Ginseng Rubra extract comparing preparation example 2 and to compare the cytoactive of mixture of preparation example 3.

With the Limax mucus of the preparation example of 0 ~ 1% (w/v), compare preparation example 1 Limax mucus, compare the Radix Ginseng Rubra extract of preparation example 2 and compare the mixture process fibroblasts of adult human dermis (HDF) of preparation example 3.This sample is not added to matched group.Measure and carry out as follows.

1) CO in 37 DEG C and 5% in the DMEM culture medium of 0.5% hyclone (FBS) is being supplemented ₂condition under, by fibroblasts of adult human dermis (HDF) with 3 × 10 ³/ hole/0.09ml cultivates on 96 orifice plates.

2) preparation example and the Limax mucus comparing preparation example are added into fibroblasts of adult human dermis (HDF) ultimate density to be adjusted to 0.25%, 0.5% and 1% (w/v), and cultivate 24 hours subsequently.

3) each hole is added to the CCK-8 solution of 10 μ l.

4) carry out cultivating for 2 hours.

5) absorbance at 450nm place is measured.

Calculate cytoactive according to equation 1 below, and result illustrates in table 2.

[equation 1]

Cytoactive (%)=[(B-A)/A] × 100

A: not containing the absorbance in the hole of sample

B: containing the absorbance in the hole of sample

Table 2

As from upper table 2, and according to comparing compared with the Limax mucus of preparation example, Radix Ginseng Rubra extract or its mixture, the Limax mucus obtained from the Limax of Radix Ginseng Rubra of feeding according to preparation example represents excellent cytoactive.

[test case 2: use cell culture technology assessment to stimulate remission effect]

In this test case; utilize cell culture to stimulate based on the cell death assessment caused by the sodium lauryl sulphate (SLS) as skin stimuli to alleviate, to assess and to compare the Limax mucus obtained from the Limax of the Radix Ginseng Rubra of feeding of preparation example, the Limax mucus comparing preparation example 1, the Radix Ginseng Rubra extract comparing preparation example 2 and compare the skin irritation remission effect of mixture of preparation example 3.

SLS is a kind of material, and it serves as the skin irritant index for assessment of cosmetic material and is attached to cell membrane, and causes skin irritation by T suppression cell metabolism and destruction cell membrane.

In this test case, wherein fibroblasts of adult human dermis is added the Limax mucus of SLS and preparation example situation and wherein SLS is added to fibroblasts of adult human dermis and compare preparation example Limax mucus situation between the effect of cell death that caused by SLS of minimizing, and its effect to be assessed.

Hs68 fibroblasts of adult human dermis for this test case is from ATCC (American type culture collection, serial number: the application on human skin hypodermal cell 1635) obtained.

Utilize the stimulation of cell culture to alleviate test to carry out as follows.

By people source dermal fibroblast with 1 × 10 ⁵the density of individual cells/well is layered in 96 orifice plate incubator, cultivate 24 hours, that compares preparation example 1 ~ 3 with the combination of Limax mucus of the SLS of independent 0.01%, the SLS of 0.01% and 0.1% preparation example and the SLS of 0.01% and 0.1% is combined into row relax, and cultivates 24 hours further subsequently.

After culturing, add MTT (Sigma) reagent, carry out the cultivation of 4 hours, remove and cultivate reaction, residue is added to the NaOH/ aqueous isopropanol of 1N, then stir 20 minutes, measure the absorbance at 565nm place with absorptiometer, and assess preparation example and compare the Limax mucus of preparation example 1 ~ 3 for the effect suppressing the cell death caused by SLS.

Table 3

As from upper table 3; the Limax mucus obtained from the Limax of the Radix Ginseng Rubra of feeding of preparation example, the Limax mucus comparing preparation example 1, the Radix Ginseng Rubra extract comparing preparation example 2 and the mixture comparing preparation example 3 can alleviate skin irritant material by suppressing the cell death that be caused by SLS, and described skin irritation may be worked as when applying some make up into assignment system and caused by cosmetic composition.Specifically, can find out, compared with the Limax mucus comparing preparation example, represent excellent stimulation according to the Limax mucus obtained from the Limax of Radix Ginseng Rubra of feeding of preparation example and alleviate.

[embodiment and comparative example 1 ~ 4: the preparation of cosmetics]

In the present embodiment and comparative example 1 ~ 4, the mixture using the Limax mucus obtained from the Limax of the Radix Ginseng Rubra of feeding of preparation example, the Limax mucus comparing preparation example 1, the Radix Ginseng Rubra extract comparing preparation example 2 and compare preparation example 3 prepares cosmetics.The cosmetics of preparation are cream, and its composition illustrates in table 4.

Table 4

Unit: % by weight

" B " section described in table 4 is heated and stores at 70 DEG C, " A " section is added to " B " section of storage, and by primary emulsifying for gained mixture, with mixer for well-distribution emulsifying and cool lentamente subsequently to prepare each cream (embodiment and comparative example 1 ~ 4) equably.

[test case 3: the measurement of skin moisturization]

In this test case; the cosmetics (embodiment) of preparation containing the Limax mucus obtained from the Limax of the Radix Ginseng Rubra of feeding available from preparation example, containing from compare the Limax mucus that preparation example 1 obtains cosmetics (comparative example 1), containing from compare the Radix Ginseng Rubra extract that preparation example 2 obtains cosmetics (comparative example 2), containing from comparing the cosmetics (comparative example 3) of the mixture that preparation example 3 obtains and the cosmetics (comparative example 4) containing sodium hyaluronate, and with contrast test assessment human body skin moisturization.

The cream prepared in embodiment is administered to the right side face of 40 study subjects (women in 30-40 year), in described object, the cream of preparation in comparative example 1 is administered to the left side face of 10 study subjects, the cream of preparation in comparative example 2 is administered to the left side face of another 10 study subjects, the cream of preparation in comparative example 3 is administered to the left side face of another 10 study subjects, and the cream of preparation in comparative example 4 is administered to the left side face of another 10 study subjects.Eachly use to carry out 4 weeks continuously twice daily.

With moisture of skin analyzer (Corneometer) before products applied after (0 week) and products applied (2 weeks) 2 weeks and measure the moisturizing at the test position of study subject for 4 weeks.The all study subjects participating in test in 4 weeks all do not have abnormal response.

When probes touch skin surface, the measurement of moisture of skin analyzer is applied to the electric capacity of the weak current of the sensor provided in end of probe, and measures water content thus.Along with water content increases, skin water capacity increases.

Fig. 5 illustrates the skin moisture-keeping result with the study subject of the cream process of preparation in embodiment and comparative example 1 ~ 4.

Table 5

n＝40，p<0.05

Calculate skin moisture-keeping according to following equation 2 to improve, and result is shown in following table 6.

[equation 2]

Skin moisture-keeping improves (%)=[(B-A)/A] × 100

The result of A:0 week moisturizing

The result of B:2/4 week moisturizing

Table 6

n＝40，p<0.05

As from upper table 5 and 6; all samples for testing all presents skin moisturization; and compared with the cosmetics of comparative example 1 ~ 3, the cosmetics of the Limax mucus obtained from the Limax of Radix Ginseng Rubra of feeding containing with good grounds embodiment present quite excellent skin moisturization.

[test case 4: the Wound Healing Effect in full thickness cutaneous defect model]

In this test case, assess the effect of cosmetics to wound healing in full thickness cutaneous defect experimental rat model according to embodiment and comparative example 1 ~ 3 with contrast test.

In every day, be 5 groups of rats by rat according to weight random packet, and manufacture rat through thickness trauma model at the first day of test.In the abdominal part of test animal, cause wound, and according to the visual direction of technical staff, on the left side with called after wound 1,2 and 3 successively from the top to the bottom, and on the right with called after wound 4,5 and 6 successively from the top to the bottom.

In the abdominal part of experimental rat, produce length be 10mm and width is the full thickness cutaneous defect wound of 10mm, by grouping as shown in table 8 for animal, and test material patch is used to wound.

Table 7

1,3,7,9 and 14 day after sticking patch, measure wound shrinkage and shown in table 9.

Table 8

Unit: cm ²

n＝5，p<0.05

In order to assess Wound Healing Effect, by the initial wound area digital image of the wound area of test group relative to 100 being obtained wound shrinkage at 1,3,7,9,11 and 14 day.

As the measurement result at the wound initiation wound shrinkage of latter 14 days, the area of all wounds shrinks due to wound surface and progressively reduces within the test period (14 days).

Meanwhile, the time of wound shrinkage most active test period is 4 days ~ 9 days.Total wound area relative to 100; the wound 1 that application of matched group presents the wound shrinkage of 47%; the wound 2 that application of positive drug (Asiaticoside) presents the wound shrinkage of 36%, and the wound 3 that application of the cosmetics containing the Limax mucus obtained from the Limax of Radix Ginseng Rubra of feeding presents the wound shrinkage of 61%.

Application of the wound surface of the patch of embodiment to be contracted in the 4th day be 31.37, being 61.76% at the 7th day, was 82.35% at the 9th day, was 96.08% at the 11st day, and was 99.94% at the 14th day.This means compared with matched group, positive controls and other comparative example group, the wound surface that application of the patch of embodiment presents much higher contraction (Fig. 1).

Although illustratively disclose the preferred embodiment of the present invention, but when not departing from scope of invention and spirit disclosed in appended claims, those skilled in the art will recognize that and can carry out various modification, interpolation and replacement.

Claims

1. prepare a method for cosmetic composition, described method comprises:

To the meals that Africangiant snail or housing helix are fed containing Red Ginseng;

Limax mucus is obtained from described Limax; And

Obtained Limax mucus is added into cosmetic composition.

2. the method for claim 1, wherein described Red Ginseng exists with the amount of 0.1 % by weight ~ 30 % by weight in described meals.

3. the method for claim 1, wherein obtained Limax mucus comprises chondroitin sulfate, allantoin and glycolic.

4., the method for claim 1, wherein relative to the gross weight of described cosmetic composition, the addition of the Limax mucus obtained is 0.001 % by weight ~ 30.0 % by weight.

5. cosmetic composition prepared by the method according to any one of Claims 1 to 4,

Wherein, described cosmetic composition is used for skin moisture-keeping.

6. cosmetic composition prepared by the method according to any one of Claims 1 to 4,

Wherein, described cosmetic composition is for alleviating stimulation.

7. cosmetic composition prepared by the method according to any one of Claims 1 to 4,

Wherein, described cosmetic composition has the preparation being selected from skin protection cosmetics preparation and cosmetics preparation, described skin protection cosmetics preparation is made up of toner, gel, water-soluble liquid, cream, essence, oil-in-water (O/W) type and Water-In-Oil (W/O) type Emulsion and unguentum, and described cosmetics preparation is made up of adornment, foundation cream, the agent of the screening flaw, lip pomade, lip gloss, face powder, dual-purpose muffin, eye shadow, cheek coloured silk and eyebrow pencil at the bottom of oil-in-water (O/W) type and Water-In-Oil (W/O) type.