KR101098400B1 - A method for mucus of acusta despecta sieboldiana feed red ginseng and cosmetic composition containing thereof - Google Patents

Abstract

PURPOSE: A method for preparing snail secretion is provided to ensure excellent wound healing and skin regeneration with safety to human body. CONSTITUTION: A method for preparing snail secretion for cosmetics comprises: a step of feeding feed containing 0.1-30 wt% of red ginseng to snail(Acusta despecta sieboldiana); and a step of collecting snail secretion containing chondroitin sulfate, allantoin, and glycolic acid. A cosmetic composition is used for skin sedation, atopic dermatitis treatment, or skin moisturizing. The cosmetic composition is manufactured in the form of a lotion, gel, water solution liquid, cream, essence, oil in water type and water in oil type emulsion, ointment, foundation, lip stick, lip gloss, eye shadow, and pencil.

Description

A method for mucus of Acusta despecta sieboldiana feed red ginseng and Cosmetic composition containing etc.

The present invention relates to a method for producing a cosmetic snail mucus and a cosmetic composition containing the same, more specifically, a snail fed a red ginseng ( Acusta Despecta sieboldiana ) relates to a method for obtaining a cosmetic snail mucus and a cosmetic composition containing the same.

In general, wound healing involves a complex process that involves inflammation, cell proliferation, cell migration, blood vessel proliferation, and cell matrix formation. These steps are continuous with some overlap without clear distinction, and the speed and extent of healing differs depending on the tissue healing method. That is, there are various forms of skin wounds, each of which has anatomical, physiological, pathological and microbiological factors that are related to each other. Although all these factors are involved in the wound healing process, the basics of the process are as follows. There are several ways to classify wound stages, but Johnston (1977) divides it into four phases: inflammatory, debride, repair and mature. It should be noted that the characteristic of the wound healing process is that the cycle of abnormal cycles is continuous.

In general, when the tissue is damaged, cell structure, physiology, and biochemical changes occur, and the damage or defect area is completely regenerated or scarred and healed. Wound contraction is one of the important factors in the healing process of skin defect window. It is a phenomenon in which defects are reduced by contraction of granulation tissue. The mechanisms of granulation tissue contraction are not yet known, but the theory is most likely due to the contraction of myofibroblasts.

The wound healing process is a bioreaction that repairs damaged tissues. When wounds occur, blood coagulation during bleeding, fibrin exudation, phagocytosis of damaged tissue fragments by mobilization of epidermis, epidermal regeneration and granulation tissue, new capillaries and fibers Healing occurs through the fibrosis step due to the proliferation of blasts and the deposition of synthetic secreted collagen fibers by the proliferated fibroblasts (Walter and Israel, 1970).

Recent studies related to wound healing have confirmed the formation of granulocytes, collagen formation, or the coverage of epithelial cells.

On the other hand, the stratum corneum of the epidermis is also located on the outermost side of the skin while suppressing the loss of moisture and electrolyte outside the skin, thereby preventing the drying of the skin and providing a normal biochemical metabolism of the skin. In addition, it plays an important role in protecting the human body from external physical damage and chemicals or preventing the invasion of bacteria and viruses into the skin.

These skins are damaged by exposure to substances that cause skin irritation, such as sodium lauryl sulfate, dimethyl sulfoxide, croton oil, sodium hydroxide, etc. Transepidermal Water Loss, an indicator of damage, increases.

As such, studies have been conducted to alleviate the irritation by protecting the skin against substances causing skin irritation. It has been found that oil-in-water emulsions containing glycerin have the ability to protect the skin against skin irritation by sodium lauryl sulfate or dimethyl sulfoxide, and the protective cream is chronic skin. It has also been reported to have skin irritant effects on stimuli.

Meanwhile, commonly a snail ( Acusta despecta sieboldiana ) belongs to the gastropods and about 20,000 species are known in the world. Because snails are less mobile, their populations are segregated by region, resulting in more subspecies. Among them are the large (100 mm shell) Achatina achatina and the Vallononia costata, which is about 1 mm high, while the large snails in Brazil have an egg length of 27 mm and a diameter of about 12 mm. to be. It is known to be distributed in temperate and tropical regions such as Korea, Japan, and China.

Mucin is composed of mucin (mucin) and mucin is composed of chondroitin sulfate (chondroitin sulfate). The main nutrients are known as proteins (54.29%), lipids (4.18%), carbohydrates (30.45%), and ash (4.07%). Allantoin, the main ingredient of snail mucus, is used for soothing and shrinking pores. It is known that glycolic acid (Glicolic Acid) has a skin trouble prevention effect and collagen (Collagen) and elastin (Elastin) are known to maintain skin elasticity.

On the other hand, Panax ginseng is a perennial herb that belongs to the Araliaceae family, and its root is called Ginseng Radix in oriental medicine and its tonic, gangjeong, hematopoiesis, and warmth are guaranteed. ), Health, health, fatigue, mental stability, sedative effects are known. The main active ingredients of ginseng include saponins, sapongenins, polyacetylenes, pyrazine derivatives, maltol, and the like, and ginseng saponins are based on the chemical structure of protopanaxadiol and protopanaxatriol. It is divided into system and oleanonane-based saponins. To date, 19, 10 and 1 compounds have been separated and purified, and diol and triol are known to have different pharmacological effects in the body. Ginseng saponins, unlike other plant saponins, are not toxic and do not show hemolytic action below 0.001%. They are reported to have central nervous system suppression, protein synthesis promotion, adrenal cortex hormone secretion, and immune boosting. It is becoming.

Meanwhile, red ginseng refers to processed ginseng made by repeating the steaming process of steaming and drying steamed ginseng without peeling it. Red ginseng is characterized by a very high content of physiologically active substance per gram of moisture due to evaporation of moisture as well as a visual difference of light yellowish brown or light reddish brown. It is known that red ginseng converts saponin glycosides to saponins during steaming to increase the concentration of saponins and to increase the species to 32 species.

On the other hand, the present inventors have studied the applicability to cosmetics in a variety of natural products, the snail (Acusta despecta sieboldiana), And the result of measuring wound healing, skin regeneration effect and skin irritation effect in snail mucus obtained by supplying red ginseng to snail was very good. It was found that can be expected to complete the present invention.

An object of the present invention is to provide a method for producing a snail mucus excellent in wound healing, skin regeneration effect, skin irritation relief effect.

In addition, an object of the present invention is to provide a cosmetic composition that can be applied to the skin cosmetic using the snail mucus obtained from the snail fed red ginseng and is harmless to the human body and has excellent safety.

In addition, the present invention provides a multi-functional cosmetic composition having excellent skin regeneration effect and stimulating effect by using the snail mucus obtained from the snail fed red ginseng, excellent skin moisturizing effect, cell activation effect, wound healing effect, atopic improvement effect It aims to do it.

In addition, an object of the present invention is to provide a cosmetic composition using the snail mucus obtained from the snail fed red ginseng not only has excellent skin regeneration effect but also has an irritation-reducing effect and exerts an excellent skin improvement effect.

In order to achieve the above object, the present invention comprises the steps of obtaining a snail ( Acusta despecta sieboldiana ) fed red ginseng; And a step of obtaining mucus from the snail.

In addition, in the present invention, the red ginseng is characterized in that the powder of 50 ~ 200 mesh size.

In the present invention, it is characterized in that for feeding the feed containing the red ginseng in an amount of 0.1 to 30% by weight.

In addition, the present invention is fed snail red ginseng ( Acusta despecta sieboldiana ) is characterized by a cosmetic composition containing 0.001 to 30.0% by weight of the snail mucus obtained from the total weight of the composition.

In addition, the present invention is characterized in that for wound healing and skin regeneration.

In addition, the present invention is characterized in that the stimulation for relaxation.

In addition, the present invention is characterized in that for improving atopy.

In addition, the present invention is characterized in that the skin moisturizing.

In addition, the present invention is a base cosmetic formulation consisting of a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type emulsion, ointment; Color cosmetic formulation consisting of oil-in-water and water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, cheek color, eyebrow pencils; It is characterized in that any one selected from.

Hereinafter, the means for solving the problems of the present invention will be described in detail.

The method for producing cosmetic snail mucus of the present invention is characterized by obtaining mucus from a snail fed red ginseng. The snail mucus obtained from the snail fed red ginseng may have excellent wound healing and skin regeneration effect by exerting excellent cell activity and skin moisturizing and atopic skin regeneration effect than conventional snail mucus.

The snail mucus obtained from the snail fed the red ginseng of the present invention contains chondroitin sulfate, allantoin, glycolic acid, and the like as compared to the conventional snail mucosa, so as to heal wounds and regenerate the skin. , Skin irritation relieving effect is very excellent.

In addition, in the present invention, the red ginseng is characterized in that the powder of 50 ~ 200 mesh size, when the size of the red ginseng powder in the above range, the snail effectively ingests the red ginseng to effectively the cell activity and skin moisturizing, atopic skin regeneration effect It becomes possible to obtain sufficient snail mucus exerted.

In addition, the present invention is characterized in that the feed to the snail containing 0.1 to 30% by weight, preferably 7 to 10% by weight of red ginseng in the snail feed, when less than 0.1% by weight the snail is not enough to ingest the red ginseng In the case of more than 30% by weight, the increase in the effect on the content is small and it is not economical.

On the other hand, the cosmetic composition of the present invention is a snail fed a red ginseng ( Acusta It is characterized by containing the snail mucus obtained from despecta sieboldiana ) as an active ingredient.

In addition, the snail mucus obtained from the snail fed red ginseng contained in the cosmetic composition of the present invention is not limited to a specific range, but preferably 0.001 to 30% by weight based on the entire cosmetic composition. When the snail mucus is contained within the above range, the effect of the snail mucus obtained from the snail fed the red ginseng is sufficiently exhibited without compromising the quality aptitude of the cosmetic composition.

On the other hand, the cosmetic composition according to the present invention, in addition to the snail mucus obtained from the snail fed the red ginseng contains other ingredients and the like that can give a synergistic effect to the main effect within the range that does not impair the main effect of the present invention. It is okay.

The compositions according to the invention contain auxiliaries commonly used in the cosmetic field, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, fragrances, fillers, blockers, pigments, odorants and dyes. can do. The amounts of these various adjuvants are amounts commonly used in the art, such as 0.001 to 30% by weight relative to the total weight of the composition. In any case, the adjuvant and its proportions will be selected so as not to adversely affect the desirable properties of the cosmetic composition according to the invention.

On the other hand, the cosmetic composition of the present invention is excellent in skin moisturizing effect, cell activation effect, atopy improvement effect, so very excellent skin regeneration and wound healing effect, for skin regeneration, wound healing, skin irritation relief, atopic improvement, Can be used for skin moisturizing.

On the other hand, the formulation of the cosmetic composition of the present invention is preferably a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type emulsion, ointment base cosmetic formulation, water It is preferred to be any one selected from the color cosmetic formulations consisting of type and water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, teak color and eyebrow pencils.

It may also be applied to the skin, optionally in the form of an aerosol, may be in the form of a solid such as a stick, and may be used as a skin care product and / or makeup product.

The present invention can provide a method for producing a cosmetic snail mucus excellent in wound healing and skin regeneration effect, skin irritation relief effect.

In addition, the present invention can be applied to the skin cosmetic using the snail mucus obtained from the snail fed red ginseng and can provide a cosmetic composition excellent in safety because it is harmless to the human body.

In addition, the present invention provides a multi-functional cosmetic composition having excellent skin regeneration effect and stimulating effect by using the snail mucus obtained from the snail fed red ginseng, excellent skin moisturizing effect, cell activation effect, wound healing effect, atopic improvement effect can do.

In addition, the present invention can provide a cosmetic composition using the snail mucus obtained from the snail fed red ginseng is not only excellent skin regeneration effect, but also has an irritating effect to exert an excellent skin improvement effect.

1 is a graph showing the results of LC-MASS analysis of the snail mucus prepared in the present preparation.
Figure 2 is a graph showing the LC-MASS analysis of the snail mucus prepared in Comparative Preparation Example.
Figure 3 is a photograph showing the change of the wound over time for the experimental mice of the skin delamination model (A is the control group, B is the present example).
Figure 4 is a photograph showing the results of H & E staining (Hematoxylin & Eosin stain) for experimental mice in the skin delamination model (A is the control group, B is the present example (snail mucus obtained from red ginseng fed snail), C is a positive control group (madecassol) )).
5 is a photograph showing the result of MT's trichrome stain for experimental mice of the skin delamination model (A is a control group, B is a positive control group, C is the present example (snail mucus obtained from a snail fed a red ginseng)) .

Hereinafter, the present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to the following examples, and includes all modifications of equivalent technical spirit.

Manufacturing example  : Preparation of snail mucus from snail fed red ginseng

Red ginseng 1-5 years old was selected as a sample, sufficiently washed and ground to make fine using 50 meshes. This was added to the general snail feed 0.1-30%, preferably 7% -10% was fed to the snail. The snail was selected only for snails grown for 5-6 months, and the size is 5-10 cm, preferably 6-8 cm. The feeding of red ginseng to the selected snails continued for 1 to 4 times for 150 to 180 days.

For a period of time, the snails, which had eaten red ginseng, were washed in running water, and only similar sizes (6 ~ 8cm) were selected, sterilized thoroughly with 70% ethanol, and weighed to obtain accurate mucus.

The snail was put into a black net and collected for 6 hours, and then fed again. After giving a 12-hour recovery period, the slime was collected in the same manner as above, and the collected slime was refrigerated.

compare Manufacturing example  : Obtaining Snail Mucus

Snail mucus was obtained in the same manner as in Preparation Example except that the snail feed did not contain red ginseng powder.

Table 1 shows the components of the snail feed used in the preparation and comparative examples.

Manufacturing example Comparative Production Example Rice bran 27-30% 37% Investigation 20% 20% Calcium carbonate (limestone) 20% 20% Tofu business 23% 23% Red ginseng 7-10% 0% Total content 100% 100%

Test Example  1: comparison of components of snail mucus

In this test example, the snail mucus obtained from the snail fed the red ginseng of the preparation example and the components of the snail mucus of the comparative preparation were analyzed by LC-MASS.

Figure 1 shows the LC-MASS analysis of the snail mucus obtained from the snail fed the red ginseng of the present preparation, Figure 2 shows the LC-MASS analysis of the snail mucus of the comparative preparation.

As shown in Figures 1 and 2, the snail mucus obtained from the snail fed the red ginseng of the present example contains abundantly the main ingredients, chondroitin sulfate, allantoin, glycolic acid, and the like. Could know.

Test Example  2: cell activity effect measurement experiment

In this test example, in order to confirm the cell activity effect of the snail mucus obtained from the snail fed the red ginseng of this Preparation Example and the snail mucus of the Comparative Preparation Example, the cell activity effect was measured under laboratory conditions.

Human dermal fibroblasts (HDFs) were treated with 0-1% of the snail mucus of this example and the snail mucus of the comparative example. As a control, one without addition of a sample was used. The measuring method was performed as follows.

① Human dermal fibroblast (HDF) was added to DMEM medium containing 0.5% FBS (fetal bovine serum) at 37 ° C and 5% CO ₂ in 96-well plates at 3x10 ³ /well/0.09 ml for 24 hours. Incubated.

② The final concentration of 0.25, 0.5, 1% in a culture medium diluted in the test substance and the comparative example of the snail mucus of 0.01 mg of the prepared test material was added and then incubated for 24 hours.

③ 10 μl of CCK-8 solution was added to each well.

④ Incubated for 2 hours.

⑤ Absorbance was measured at 450 nm.

The cell activity effect was calculated by the following Equation 1, the results are shown in Table 2.

A: absorbance of the well without sample

B: absorbance of the well to which the sample was added

Sample Name Treatment concentration (%) Cell activation effect (%) Snail mucus obtained from snail fed red ginseng
(Production example) 0.25 28.0 0.5 32.5 1.0 39.5 Snail mucus
(Comparative Production Example) 0.25 19.5 0.5 26.5 1.0 30.0

As shown in Table 2, it was confirmed that the snail mucus obtained from the snail fed the red ginseng of this Preparation Example had a superior cell activity effect than the snail mucus of the Comparative Preparation Example.

Test Example  3: Evaluation of Stimulation Relaxation Effect Using Cell Culture Technology

In this test example, sodium lauryl sulfate, a substance that induces skin irritation using cell culture technology, was used to compare and evaluate the skin irritation-relieving effect of the deswelling mucus obtained from the snail fed the red ginseng of this preparation and the snail mucus of the comparative preparation. Stimulatory effect was evaluated to the extent of inhibiting apoptosis by Sodium Lauryl Sulfate (SLS).

SLS is a substance that is used as a criterion for evaluation of skin irritation based on cosmetics, and is a substance that binds to cell membranes to inhibit cell metabolism and destroys cell membranes to cause skin irritation.

This test example compared and evaluated the effect of reducing the cell death by SLS when SLS and the snail mucus of this manufacture example were added to human fibroblasts, and the snail mucus of the comparative manufacture example was added.

The Hs68 fibroblasts used in this test example were human dermal dermal cells and were used by the ATCC (American Type Culture Collection, Accession No .: 1635).

Stimulation relaxation test using the cell culture technology was carried out as follows.

Human-derived fibroblasts were dispensed at a concentration of 1 X 10 ⁵ per well in a 96-well plate incubator, and cultured for 24 hours, followed by 0.01% SLS alone, 0.01% SLS and 0.1% snail mucus, 0.01% SLS and 0.1 The snail mucus of% Comparative Preparation was treated and further incubated for 24 hours. After incubation, MTT (Sigma) reagent was added and incubated for 4 hours, the culture solution was removed, 1N NaOH / isopropanol solution was added, stirred for 20 minutes, and the absorbance was measured at 565 nm with an absorbance spectrometer. The effect of inhibiting apoptosis by SLS by the snail mucus of the comparative example was measured.

 Sample Name  Fibroblast survival rate (%) 0.002% SLS 43 0.002% SLS + 0.1% Snail mucus of this preparation 82 Snail mucus of 0.002% SLS + 0.1% comparative preparation 71

As shown in Table 3, the snail mucus obtained from the snail fed the red ginseng of the present preparation and the snail mucus of the comparative preparation were skin which may occur as a cosmetic base when prescribed with the cosmetic base by inhibiting apoptosis caused by SLS. It was found to be a stimulant-releasing substance that relieves stimulation. In addition, it was confirmed that the snail mucus obtained from the snail fed the red ginseng of the present preparation, in particular, has a very good stimulating effect as compared to the snail mucus of the comparative example.

Example  And Comparative example  1, 2: Cosmetics  Produce

In the present Examples and Comparative Examples 1 and 2, cosmetics were prepared using the snail mucus obtained from the red ginseng-fed snail obtained in Preparation Example and the snail mucus of Comparative Preparation Example. The prepared cosmetics are in the form of a cream, the composition is shown in Table 4.

Unit: weight% Raw material Example Comparative Example 1 Comparative Example 2 end





Stearyl alcohol 8 8 8 Stearic acid 2 2 2 Stearic Acid Cholesterol 2 2 2 Squalane 4 4 4 2-octyldodecyl alcohol 6 6 6 Polyoxyethylene (25 mol addition) alcohol ester 3 3 3 Glyceryl Monostearic Acid Ester 2 2 2 I



Snail mucus fed red ginseng 5 - - Snail mucus of comparative manufacture example - 5 - Na-hyaluronic acid salt - - 5 Propylene glycol 5 5 5 Purified water Rest about 100 Rest about 100 Rest about 100

The 'Na' phase recorded in Table 4 was heated and stored at 70 ° C. The preliminary emulsification was performed by adding the 'ga' phase to the preserved 'na' phase, and then uniformly emulsified with a homomixer, and then gradually cooled. Creams (Examples and Comparative Examples 1 and 2) were prepared.

Test Example  4: skin moisturizing effect measurement

In this test example, cosmetics containing snail mucus obtained from the red ginseng snail obtained in this example (Example), cosmetics containing snail mucus obtained in Comparative Preparation Example (Comparative Example 1) and Na-hyaluronic acid salt The cosmetics (Comparative Example 2) containing were prepared, and the skin moisturizing effect was evaluated by performing a comparative experiment on humans.

Thirty experimenters (females in their 30s and 40s) were compared to the cream prepared in Example on the right side of the face, and the cream prepared in Comparative Example 1 on the left side of the face of 15, compared to the left side of the other 15 faces. The cream prepared in Example 2 was applied twice a day for four consecutive weeks. Moisturizing of the test site of the subject before (0 week), 2 weeks and 4 weeks after product application was measured using a skin moisture meter (Corneometer). There were no adverse events in all subjects who participated in the 4-week trial.

The skin moisture meter measures the capacity of the minute current that is conducted to the sensor embedded in the probe's head when the probe is in contact with the surface of the skin and indicates the moisture content. This means a lot.

Table 5 shows the skin moisturizing results of the subjects using the creams prepared in Examples and Comparative Examples 1 and 2.

Product application period 0 2 4 Example 64.26 73.54 77.42 Comparative Example 1 64.74 70.16 74.88 Comparative Example 2 63.84 68.96 72.60

n = 30, p <0.05

Skin moisturizing improvement rate was calculated by the following equation 2, the results are shown in Table 6.

A: 0 days moisturizing measurement result

B: Moisture measurement result for 2 or 4 weeks

Product application period 2 4 Example 14.44 20.48 Comparative Example 1 8.37 15.66 Comparative Example 2 8.02 13.72

n = 22, p <0.05

As shown in Tables 5 and 6, all of the tested samples showed skin moisturizing effect, and the cosmetics containing the snail mucus obtained from the snail fed the red ginseng of the present example was higher than the cosmetics containing the snail mucus of Comparative Example 1. It was confirmed that it has a very good skin moisturizing effect.

Test Example  5: atopy improvement effect

In this test example, the cosmetics of Example and Comparative Example 1 were evaluated by performing a comparative experiment on the effect of improving atopy in people with atopy.

Twenty test subjects (ages 3 to 40) with atopic dermatitis were administered to the atopic dermatitis in 10 patients and the cream prepared in Comparative Example 1 to the remaining 10 people twice a day. Application was made for 4 weeks. Atopic dermatitis severity, IgE change, coral white blood cell (Eosinophil) change, transdermal moisture evaporation, moisture content, and skin acidity were measured before and after product application (0 week) and 4 weeks after product application, respectively. There were no adverse events in all subjects who participated in the 4-week trial.

Table 7 shows a comparison of the atopy improvement effect of the experimenter using the cream prepared in Example and Comparative Example 1.

Sample Name Atopic Dermatitis Severity IgE change Coral Leukocyte Changes Transdermal moisture
Evaporation Water content Skin acidity I'm after I'm after I'm after I'm after I'm after I'm after Example 37.14 29.46 436.4 319.7 306 245.3 37.00 25.55 25.48 26.68 5.729 6.048 Comparative Example 1 37.84 32.45 446.7 367.1 265 327.3 32.19 28.36 26.50 25.07 5.705 5.811

n = 20, p <0.05

As shown in Table 7, the snail obtained from the snail obtained from the red ginseng fed snail of the present invention was the severity of atopic dermatitis, the change of IgE and coral leukocytes in blood, the evaporation amount of transdermal water, moisture content, skin acidity, etc. It was found that the snail mucus of the present invention was excellent in the atopic improvement effect in the experimenter who applied the cream containing mucus.

Test Example  6: skin All floors  Wound Healing Effects in a Defective Model

In this test example, the cosmetics of Example and Comparative Example 1 were evaluated by performing comparative experiments on the wound healing effect in the skin delamination model in experimental rats.

Five rats per day, and the last 14 days of the test group were divided into groups by random weight, and then a full-thickness wound wound model was prepared on the first day of the test. Wound induction was designated wounds 1 and 2 in order from the top left to the bottom of the test animal, and wounds 3 and 4 in the order from the top right to the bottom.

After making a 10 mm transverse layer defect on the back of the rat, a patch of test substance was applied, and H & E staining (Hmatoxylin & Eosin stain) and MT for wound shrinkage and histopathological examination for 1, 3, 7, 9 and 14 days respectively. Masson's trichrome staining was performed to evaluate the healing effect in the skin delamination wound model.

The groups were separated as in Table 8 and the samples were administered.

Sample Name Dosage Application area by animal Control group blank patch Wound 1 Positive control group (madecassol) 500 mg / cm ² Wound 2 Example 500 mg / cm ² Wound 3 Comparative Example 1 500 mg / cm ² Wound 4

Test Example  6-1: Wound Shrinkage Measurement

In order to quantify the wound healing effect, wound shrinkage was obtained by digitally imaging wound areas at 3, 7, 9, 11 and 14 days after 1 day in the autopsy group with an initial wound area of 100.

3 is a photograph showing the change of the wound over time, A is the case of the patch of the control group, B is the case of the patch of the embodiment.

When the wound shrinkage rate was measured for 14 days after the wound induction, as shown in FIG. 3 and Table 9, all wounds showed a result of gradually decreasing the window surface by the window shrinkage during the test period (14 days).

On the other hand, the most active wound contraction period was between 4 and 9 days. When the total wound area was 100, 45% of the wounds with the control group were 45%, wound 2 with the positive drug (madecassol) was 33%, and red ginseng was fed. The wounded wound 3 containing the snail mucus obtained from the snail had 51% contraction.

Among them, the shrinkage rate of the wound surface with the patch of Example was 30.69% for 4 days, 60.62% for 7 days, 81.93% for 9 days, 95.469% for 11 days, and 99.941% for 14 days, showing very high shrinkage compared to the control group and the positive control group. Could know.

1 day 4 days 7 days 9 days 11 days 14 days Control group 0.99 0.73 0.52 0.28 0.11 0.02 Positive control group (madecassol) 1.06 0.87 0.67 0.51 0.33 0.12 Example 1.02 0.70 0.39 0.18 0.04 0.0006 Comparative Example 1 1.10 0.75 0.50 0.28 0.12 0.01

Unit: cm ²

n = 9, p <0.05

Test Example  6-2: Histological Examination

4 and 5 show the results of H & E staining (Hematoxylin & Eosin stain) and MT staining (Masson's trichrome stain) for histopathological examination, respectively. In Figure 4 A is a control group, B is an example (snail mucus obtained from a snail fed red ginseng), C is a positive control group (madecassol), in Figure 5 A is a control group, B is a positive control group, C is an example ( Snail mucus obtained from red snails fed snails).

As shown in FIG. 4, the patch-treated wound healing surface of the embodiment was formed thicker in both the 4-day autopsy group and the 7-day autopsy group and the 11-day autopsy group findings at the end of the recovery period compared to other patch-covered wound healing surfaces. The granulation tissue and collagen fibers proliferated in parallel with the window, and rapid regeneration of the epithelium was observed.

In addition, the effect of the patch on the collagen (collagen) was confirmed according to Figure 5, the MT stain (Masson's trichrome stain) of the wound surface on the 4th day of the ablation wound formation (Masson's trichrome stain) showed a big difference in collagen formation in all wounds There was no, but after 7 days it was confirmed that the collagen formation was significantly increased compared to the other wound surface in the wound surface of the patch.

Claims (9)

Feeding a snail ( Acusta despecta sieboldiana ) a feed containing 0.1-30% by weight of 50 mesh red ginseng powder; And
Obtaining a snail mucus comprising chondroitin sulfate, allantoin and glycolic acid from the snail; Cosmetic snail mucus, characterized in that it comprises a. delete delete A snail ( Acusta despecta sieboldiana ) fed a feed containing 0.1-30% by weight of red ginseng powder of 50 mesh size and containing chondroitin sulfate, Allantoin and Glycolic Acid Cosmetic composition characterized in that it contains 0.001 to 30.0% by weight relative to the total weight of the composition. delete The method of claim 4, wherein
Cosmetic composition, characterized in that for stimulation relief. The method of claim 4, wherein
Cosmetic composition for improving atopy. The method of claim 4, wherein
Cosmetic composition, characterized in that for moisturizing the skin. The method according to any one of claims 4 and 6 to 8,
The cosmetic composition is a cosmetic composition consisting of a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) and water-in-oil (W / O) type emulsion, ointment; Color cosmetic formulation consisting of oil-in-water and water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, cheek color, eyebrow pencils; Cosmetic composition, characterized in that any one selected from.

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