TWI679028B - Chinese herbal medicine composition with skin epidermal stem cells caring function and mask using the same - Google Patents

Abstract

Epidermal stem cells used for skin maintenance, Chinese herbal medicine composition with multiple functions, and Mask containing grape skin or Polygonum cuspidatum root extract 5wt% ~ 11wt%, mulberry leaf extract 0.5wt% ~ 1.1wt%, scutellaria baicalensis extract 5wt% ~ 11wt%, tripterygium root extract 0.7wt% ~ 1.3wt%, Brown algae extract 3wt% ~ 9wt%, rose extract 1wt% ~ 5wt%, Rhodiola rosea extract 1wt% ~ 5wt%, licorice extract 0.5wt% ~ 6.5wt% and additives 51wt% ~ 83wt%, and it has maintenance The effect of skin epidermal stem cells.

Description

Epidermal stem cells for skin care and Chinese herbal medicine group with multiple functions Product and its mask

The invention relates to a Chinese herbal medicine composition, in particular to an epidermal stem cell for skin maintenance, a Chinese herbal medicine composition with multiple functions and a mask thereof.

Human skin aging or dry skin, dullness, spots, wrinkles, rough, scars, sagging, fatigue, sagging, thick pores, etc. come from various genetic factors, living environment, eating habits, drugs, endocrine, behavior and time, etc. The influence of the problem was formed, and various scholars have various opinions for various reasons. However, the root cause is the source, that is, the epidermal stem cells are growing and aging with the years, so their functions are decreasing and the number is decreasing, and they are damaged by external factors such as living environment, eating habits, drugs, behaviors, etc. Injured. That is, the epidermal stem cells show the phenomenon of aging, senescence, decline, decay and fading, which leads to defects in skin beauty. Observe human skin evolution during infancy, childhood, infancy, youth, adulthood, middle-aged, middle-aged, and old age. The operation of the main epidermal stem cells shows five causes and effects. It is also called irreversible "skin physiological clock "phenomenon.

In order to improve the existing defects of the skin or prevent skin beauty defects and photo-aging, natural aging, or to make the skin beautify, people use a variety of different beauty care products, and indeed achieve a certain degree of beauty effect. However, most beauty care products in the market cannot penetrate the skin and can be absorbed by the body due to its molecular weight exceeding 5,000 Daltons (about 1.67 x 10 ^-27 kg). It can only stay on the skin layer, making it like hitting Wax, the surface looks smooth, but it will not change the essence of the article, it can only be regarded as a beauty care product that treats the symptoms and cannot cure the root cause. Some beauty care products use materials with small molecular weight that can penetrate into the skin to be absorbed by the human body. However, if you cannot increase the life of human epidermal stem cells, there are still regrets. The facial mask in the current market shows this phenomenon, but it has been loved by people who love beauty. Taiwan TV media have reported enthusiastically that Taiwan ’s facial mask accounts for 17% of the world ’s sales, with annual sales of more than 100 million pieces, and is a popular tourist for mainland China. Come to Taiwan to grab the top spot in the shopping list. Obviously, the mask has become a daily necessity for beauty and maintenance of modern women, but if men can also use it as a beauty and maintenance essential, it will be a further breakthrough in the use of masks.

The inventor, Weng Ming's family, once served as a defender of Jiang Song Meiling, and had a good personal relationship with his personal waitress, Ye Qiuliang, so he knew the secret recipe of his palace mask, so he cited it as the secondary component of the invention and shared it with the world. The inventor Weng Mingjia has been the chairman / general manager of Taiwan Acer Cord Blood Bank, the chief consultant of Achieve Cord Blood Bank, a shareholder of cutting-edge cord blood bank, and has practical familiarity with the field of stem cell applications. State Intellectual Property Office ZL201210191413.3 and US Patent US 9439874; and the inventor of "Food Formula for Nutrient Maintenance and Repair of Various Stem Cells and Manufacturing Method" No. I563922 of the Republic of China Intellectual Property Office. The demands are limited to one or two of moisturizing, whitening, blemish, wrinkle removal, etc. None of them appealed for the above-mentioned multi-functional and complete mask. The inventor Weng Mingjia decided to add all four functions and add all the additional features. It has flat marks and anti-aging skin functions, and its formula and manufacturing method are particularly useful for proliferating, activating, repairing, moisturizing and regulating human epidermal stem cells. Innovative facial mask beauty care products, combined with the secret mask of the palace mask and the modern application A new facial mask made of bio-humanized epidermal stem cells in the field of biomedicine is a feature of this invention and will benefit the world more widely in the future. People, not only women, will love to use it, but "men even love their younger skin".

The original mask cleans the skin mainly through adsorption. When the mask is applied to the skin, a cover film is formed and peeled off after about 20 minutes. At this time, the sebum and dirt in the hair follicles are peeled off with the mask, which makes the pores open and is beneficial to Excretion of sebum and osmotic absorption of nutrients to achieve a single appeal or two There are six kinds of demands for moisturizing, whitening, mottled spots, wrinkles, flat marks, and anti-aging of the skin. The present invention leads to a variety of demands for a facial mask with six functions; especially the leading edge can be used for maintenance The formula of human epidermal stem cells and its manufacturing method are expected to emerge in the face mask market today, and contribute to the beauty of the world and the hope of youthful skin.

Earthworms can be divided into two if they are not cut to death. Geckoes and crickets can still regenerate their tails. These animals have natural regenerative stem cells, while humans' regenerative organs are currently only found in the liver and hair. , Nails, skin, which are classified as skin stem cells except the liver. Skin stem cells were published by Nobuyuki Yamanaka of Kyoto University in Japan in 2006. The world's first use of mice to generate "iPS cells from all-purpose cells" from adult skin. 2007. James Thomson, University of Wisconsin, USA, with Nobuya Yamanaka on November 20. Mi was published in two different units on the same day: skin cells were transformed into human iPS universal stem cells, and these stem cells were successfully transformed into part of the body organs. By implanting specific four genes into skin cells, skin cells can be induced It was transformed into a kind of universal stem cell similar to "embryonic stem cells", and later both won the Nobel Prize for invention. It shows that skin stem cells are the universal stem cells after embryonic stem cells. In April 2016, Japan's Physicochemical Research, so iPS cells cultured artificial skin containing accessory organs such as hair follicles, sweat glands, and sebaceous glands. In the past, artificial skin did not have the above phenomenon. After transplanting to experimental mice, hair was successfully grown, which is expected Applied to humans within 5 to 10 years, it is used to treat burns, severe baldness and other diseases. The function of skin stem cells has been showing new breakthroughs.

Skin stem cells are mainly divided into two categories: "epidermal stem cells" and "hair follicle stem cells". Epidermal stem cells are multipotent stem cells in skin tissue. They are adult tissue stem cells that can proliferate and differentiate into various cell components in the epidermis and maintain the normal epidermal structure of the skin. Epidermal stem cells were isolated from the basal layer of the epidermis of the skin. In addition, scientists also found that stem cells were also present at the bulges on the side of the hair follicles below the epidermis, defined as hair follicle stem cells, and confirmed that these stem cells can not only generate hair follicles, but also Develop into epidermis and sebaceous glands. The epidermal stem cells, which have been studied more and more deeply among the skin stem cells found, account for only 1% to 10% of the underlying cells of the epidermis, but they are important for maintaining skin metabolism and wound repair. Significant research has shown that epidermal stem cells are the key source of skin formation, repair, and remodeling. The repair of skin damage, defects, and defects caused by various causes depends on the differentiation, proliferation, migration, and fusion of epidermal stem cells to achieve recovery. The more purpose.

Most of the epidermal stem cells in the body are in a resting state. Only when the skin is damaged or cultured in vitro, the cell division and proliferation can be significantly accelerated. Under normal circumstances, each epidermal stem cell can generate a stem cell by "asymmetric division" And a directional progenitor cell is transiently expanded cells (TA). In the epidermis, the main function of TA cells is to increase the number of terminally differentiated cells. Second, similar to directional progenitors of the hematopoietic system, transiently expanded cells (TA) differentiation potential Limited research shows that it can only differentiate into keratinocytes, hair, and sebum in the epidermis. Both asymmetric divisions exist in the basal layer of the epidermis, and the spinous layer and keratin are formed with the terminal differentiation of epidermal stem cells. Layer cells. Stem cells, TA cells, and terminally differentiated cells are distributed in different spatial structures of the epidermis, collectively referred to as "epidermal proliferation unit (EPU)", and they change in a gradient. The other is "asymmetric division of cell subsets", also known as "symmetric division", that is, two stem cells or two progenitor cells can be generated each time. In this way, when the epidermal tissue is damaged by external damage, stem cells can be increased by symmetrical division Or the number of differentiated cells to maintain the stability of the epidermis and the need for a better response to the organism. In short, each daughter can produce two daughter cells, one of which has the original characteristics of a stem cell, and the other daughter cell differentiates into a transient expansion cell (TA). This TA forms mitotic cells after 3 to 5 divisions. After mitosis, the cells continue to change upward into "terminally differentiated cells" on the basal layer, and finally fall off the skin surface with the formation of "dander". That is, the ancients said that people are made of clay. Every day, if you wash your face and bath, your skin will still have dirt that can never be cleaned!

The source of epidermal stem cells may be: (1) induced differentiation of embryonic stem cells; (2) reverse production of differentiated cells; (3) regeneration of mesenchymal stem cells into epidermal stem cells; (4) stem cells at the hair follicles are a reservoir of epidermal stem cells; (5) hematopoietic Stem cells or other tissues Stem cells migrate with the blood circulation or pass through to skin tissues. Under the stimulation of certain factors, due to the plasticity of stem cells, they differentiate laterally to epidermal stem cells. Cuticle dry Cells have three of the most prominent characteristics: slow periodicity (terminal differentiation), self-renewal ability, and adhesion to the basement membrane of the skin. This makes epidermal stem cell growth cycle, growth latency, logarithmic growth phase and plateau phase longer than epidermal cells, and the self-renewal ability of epidermal stem cells not only keeps the cell's unlimited proliferation ability, but also naturally differentiates into epidermal cells, increasing and maintaining the viability of living cells. Quantity.

Skin stem cells have been refined and developed into universal stem cells that have functions similar to human fetal stem cells, and have become the focus of research on tissue-engineered skin in medicine, because how many injuries are admitted to the hospital each day for burns, scalds, trauma and other wounds that cannot be healed. For patients, the source of skin peeling is also in short supply. In the United States, 600,000 people suffer from diabetes ulcers for a long time. 600,000 people have to cut their skin to treat skin cancer, and 10,000 to 15,000. A person needs a skin transplant to treat severe burns. How many surgical operations are expected to heal the wound quickly, how much baldness is eager for hair regeneration, and how much white hair is for black hair? In contrast, for the most common skin beauty, the skin will age, lose elasticity and show wrinkles, no longer delicate and smooth, etc. Because of the skin's complexion, luster, moisture, delicateness and elasticity, it has become the beauty of modern civilization. The skin is exposed to the effects of mechanical stress, chemical irritation, ultraviolet radiation, and microbial invasion. It continuously grows and renews through the mitosis of skin stem cells. The differentiation of skin stem cells can also be affected by exogenous factors such as its surrounding tissues and extracellular matrix, such as: stimulating growth factor, KGF (keratinocyte growth factor), EGF (epidermal growth factor), TGF (transforming growth factor) , PDGF (platelet growth factor) and inhibitory signaling substances (such as epinephrine), etc., are involved in the regulation of skin proliferation and are related to skin restoration and repair. In 2007, Stanford University in the United States and Dior, who is committed to skin aging research The R & D center has developed "epidermal stem cell protection technology". Experimental results confirm that the protective ability of epidermal stem cells is increased by 104%, which effectively resists aging and irritating microstress injuries. In the same year, Dr. Zhang Yuanhao from the same school (the eldest son of Zhang Zhaoxiong, deputy chairman of the Taiwan People ’s Democratic Party ’s former Chang Gung Hospital) published an experimental report of “aging and rat skin rejuvenation” in the famous world journal Gene and Development. His team used A two-year-old mouse (equivalent to a person's life span 50 years or older), the mouse's skin is aging, they blocked the mouse's NFkB gene and applied it for two weeks After applying certain skin care products on the skin, the treatment will stop the skin from aging and promote the rebirth. After the skin is maintained, the gene state slowly appears the same as the skin of the newborn mice, which is very amazing.

Although skin stem cells have been found to be very versatile, there are still limitations and difficulties in the use of today's technology transplantation. The most important are the lack of sources of stem cells, the difficulty of obtaining them, and the immaturity of culture techniques. Like embryonic stem cells, although It is almighty, but the source is very difficult. Unless it can be taken by abortion or abortion, it must be sacrificed one life to save another life. It does not conform to many religious, ethical, moral and legal issues. Skin stem cells are usually In order to collect the circumcision of children, the other is to use autologous skin or allogeneic skin for transplantation or cultivation. It is necessary to sacrifice one skin to save the other skin, such as some burns or medical treatments. Allogeneic skin transplantation will have anti-host rejection considerations. Regardless of autologous or allogeneic skin, the eastern wall will still be dug to fill the western wall. In terms of beauty, young and delicate skin will be replaced, and young and vigorous epidermal stem cells will be restored. It is indeed an obstacle with many difficulties and money! Therefore, finding alternative methods has become the research focus of modern medical aesthetics.

Biomedicine's previous technology-oriented activation of skin epidermal cells is recognized as the best way to cure the disease, but they all use human or animal or chemical "cytokines" as a skin defect derived from aging, and Several related patents disclose the anti-aging application of biochemical factors, for example: 1987, Japanese patent JP62019511A2, compounding an epithelial growth factor (EGF) into cosmetics as a component to prevent skin aging, 1999, US patent US56306848, applying an organic A guanidine derivative is used as an anti-aging cosmetic ingredient to modify wrinkles. In the same year, Japanese patent JP2001097844A2 applied Thymusserphyllum as an anti-aging cosmetic ingredient. Until 2003, Taiwan's invention patent application number: 092101859, which used chemotactic cytokine components (similar to those secreted by bone marrow cells) for anti-aging cosmetics, 2006 Taiwan invention patent application number: 095149902, submitted to Lianhua amniotic membrane is used as a method of biomedical materials, such as promoting the regeneration of the epidermis of burn patients, or as a skin care mask for anti-aging and wrinkle reduction. Taiwan Invention Patent Application Number: 103105080, Using mesenchymal stem cell extracts (chemokines) for the repair of skin aging, hasn't it been revealed that it can increase humanized epidermal stem cells and inhibit skin cancer and cancer stem cells? In particular, the characteristics of cancer stem cells are the same as those of normal stem cells, and if they are not intelligent, they can proliferate stem cells. Wouldn't the cancer stem cells incubate during the incubation period and seek their own way out? It is very important!

Researchers have discovered that the use of certain herbal plant extracts, after acting on the skin, can promote skin metabolism and achieve beautification or repair of the skin through transdermal infiltration. For example, Chinese patent CN102772354A discloses a Chinese medicine whitening and spot removing mask composition It contains the extract of Scutellaria baicalensis, which is similar to Chinese patent: CN104666178A discloses a whitening and moisturizing composition and mask. It contains licorice extract and Polygonum cuspidatum extract, which have not been disclosed. The extract is effective in increasing living human epidermal stem cells, and which molecules can be extracted to increase living human epidermal stem cells, and can promote transdermal molecules, and can have a synergistic effect?

Chinese herbal medicine has a long history, and many active ingredients have been used in battles or burns and trauma since ancient times. So researchers use modern stem cell medicine to test what can have proliferative human epidermal stem cells? After many years of efforts, there are some eyebrows, but they are all focused on burns and trauma. The present invention aims at medical beauty, and seeks Chinese herbal medicine biomedical molecules that can reverse human skin rejuvenation or delay skin aging to increase human epidermal stem cells; and can improve skin imperfections, so Jiang Song Meiling Palace Royal Mask The main ingredient is the stepping stone, and the icing on the cake is to use modern human epidermal stem cells that can increase life and biomedical molecules that can prevent skin cancer and inhibit cancer stem cells and molecules that can promote skin penetration. The following existing tests show and the examples prove that the present invention has a novel, progressive and economical application and creation in the field of beauty and beauty of "medical beauty mask".

Researchers have found that certain herbal plant extracts can increase living human epidermal stem cells or epidermal cells, such as: Liu Dewu, Department of Pharmacy, Burn Center of the First Affiliated Hospital of Jiangxi Medical University in 2004. "Treatment of skin defects" study, the conclusions indicate that under the experimental conditions, Scutellaria baicalensis can induce the proliferation of epidermal stem cells, and epidermal stem cells can participate The formation of new hair follicle structures on the wound has application prospects for constructing tissue-engineered skin to completely repair skin structure and function. In 2006, Song Ning of the Liaodong College of Agriculture and Environment Branch researched "the function and application of resveratrol from grape skin extract", and its role in skin health and beauty explained that resveratrol can promote collagen secretion, maintain skin elasticity and Thickness, promote the activity of SOD, remove free radicals, anti-oxidation, improve human circulation, increase skin nutrition, reduce wrinkles, promote epidermal cell differentiation, inhibit melanin and whiten the skin. In 2009, Hu Xiang et al. Of the Department of Pharmacy, the First Affiliated Hospital of Nanchang University conducted a study on "Effect of Scutellaria Baicalensis on Proliferative Activity of Human Skin Epidermal Stem Cells". Proliferative activity (reverse aging of epidermal stem cells). In 2007, Zhejiang University People's Hospital Orthopedics ASRock and other "alginate dressing and mEGF combined application to promote the proliferation of refractory wound epidermal stem cells" research, which showed that alginate has extremely strong absorbability and can absorb 20 times its own weight Liquid, which can effectively control the leakage and prolong the use time. It can keep the wound moist and non-sticky. It is of great significance to maintain a humid environment, improve the regeneration capacity of epidermal cells, accelerate the movement of epidermal cells, and promote wound healing. (In 2015, Taiwan ’s world-famous Balibaxian amusement park collective burn news, As a result, patients were sent to the Taiwan Armed Forces General Hospital, which has developed an alginate dressing. The burn cure rate was much higher than patients in other hospitals, which became another news focus.) Researchers have also discovered that insects, crustaceans, or snakes rely on a type of "ecdysone" to husk and peel, which can stimulate the dermal cells to produce new epidermis, which is transformed, such as butterflies, silkworms, cicadas, or snakes. Representation. In 1966, K. Nakanishi and others first discovered that the plant also had this substance in Taiwan's folk anti-tumor herb "Zinzheqing", which is also an important element of why plants can return to green, sprout, or evergreen, and later in mammals, Humans have also found that it plays a role in growth and development, molting, especially in the plant world, and can be extracted in large quantities. For example, mulberry leaves have become the main food of silkworms. The molting hormone in mulberry leaves is called β-ecdysone. It is called ecdysterone. In 1974, Michael D et al. Promoted human epidermal cell differentiation through in vitro experiments. Recently, in 2009, Li Guofang et al., "Southern Hospital Affiliated to Guangzhou Southern Medical University," etc., "Effect of ecdysterone on human epidermal stem cell proliferation in vitro." research The results show that ecdysterone (EDS) can promote the proliferation of human epidermal stem cells (hESCs) under in vitro culture conditions, and this effect is most significant when the concentration of EDS is 80 mg.L-1. One of its promoting mechanisms may be that it promotes the growth of human epidermal stem cells from the G0 phase (the cells are in a dormant phase, which may temporarily or permanently stop growing) / G1 phase (the cells start to grow, the cell size increases, and RNA and synthesis are produced simultaneously). Protein, the main purpose is to prepare for DNA replication), enter the S phase (that is, the DNA synthesis phase, in order to make the two daughter cells similar after division, the content must be doubled), and then enter the G2 from the S phase Phase (the period during which DNA is copied to mitosis, at which time the DNA content in the nucleus changes from 2n to 4n in the G1 phase. There are also limiting points in the G2 phase. Check whether the DNA has completed replication, whether the cell has grown to a suitable size, and whether environmental factors are favorable During cell division, etc.) / M phase (synthesis of proteins during which the cells stop growing in the M phase), the energy concentration of all cells is complex and regular cell division to obtain two similar daughter cells), start and reverse the epidermis Stem cell activity. In 2010, Qiu Fei from Shanghai Shuguang Hospital and other researches on "Asian glycosides promote the replication and division of human epidermal stem cells" showed that: Asiaticoside has the functions of promoting collagen regeneration and accelerating wound healing, and can promote the division of human epidermal cells cultured in vitro. It can shorten the fusion time of epidermal cells, and can promote and protect the proliferation of epidermal cells. In 2010, Parker conducted a variety of comprehensive research tests. The results showed that rose leaf stem cells can not only improve the vitality and life span of skin stem cells, but also enhance the skin's barrier function and protect the skin from the stress caused by climate change. Guangzhou Lingyu Co., Ltd. also conducted research on rose leaf stem cells. The results show that rose leaf stem cells can protect epidermal stem cells and maintain the skin barrier, which is effective in removing wrinkles, anti-redness and allergies. In 2011, the study of “Differential Analysis of Resveratrol on the Protective Effect of Resveratrol on HaCaT Cells Injured by UVB” by Wang Yizhen and other Chinese Academy of Armed Police Colleges, concluded that resveratrol can up-regulate the expression of transcription-related proteins in cells, thereby protecting and promoting the cells Transcription proceeds normally. On the other hand, it also protects against medium-wave ultraviolet (UVB) by up-regulating MAGOH protein activity and promoting epidermal cell differentiation.

Researchers have discovered that a herbaceous plant naturally resists elements and antibiotics, enabling plants to face bright sun, cold winter nights, wind and rain, smoke and dew, and birds to peck, insects to gnaw, and even roots to be cricket. Invasion, and can survive, that is the resveratrol biomedical molecules found in the skin, leaves and roots of 72 kinds of plants, and the highest content is found in grape skin, Polygonum cuspidatum root, peanut buds, and mulberry leaves. After perennial medicine The continuous research trials of the family have repeatedly produced surprisingly different curative effects. For example, in 2004, Dr. Jalenti and Dr. Sinclair of MIT in the United States engaged in other anti-aging methods after food restriction therapy. The ingredients were fed with yeast, nematodes, and fruit flies. As a result, resveratrol, a biomedical molecule, can extend its life by 75%, showing that resveratrol can increase its sir2 (longevity gene) activity. Alessandro of the Italian Institute of Neurology in 2006 ^. Dr. Celleri published in the journal "Contemporary Biology": Resveratrol can increase the life span of the shortest-lived vertebrate African tooth carp (fish) by more than 50%. On November 1, 2006, Harvard University in the United States cooperated with the National Institute of Gerontology. Dr. Sinclair's research team screened 18 kinds of ingredients that can activate the sirt ~ 1 longevity gene from more than 100 kinds of ingredients that are said to be anti-aging. Resveratrol is at the top of the list, and rat experiments can extend the lifespan of mice by 15% (equivalent to a 10-year increase in human lifespan). This result has reported a worldwide sensation, and more than 5,000 media around the world have reported the magic of this resveratrol. Since 2006, further anti-aging tests have been carried out on monkeys. Because monkeys live 40 years a day and humans live 120 years a year, there is no way to determine anti-aging in such a long life. Life extension of several years, but in the laboratory of the University of Wisconsin, 20-year-old monkeys, monkeys who have resveratrol and monkeys who do not have resveratrol have passed the same age for eight years; the two monkeys are characterized by physical appearance and physical activity. Observation, monkeys who eat resveratrol are vigorous and radiant, and monkeys who do not eat resveratrol are old-fashioned. Obviously, monkeys who eat resveratrol are much younger! Another scientist made various comparative experiments on the human resveratrol dosage form. Transdermal absorption is better than white clothing. Therefore, it is more beneficial to the human body for the application of beauty care products.

Researchers have discovered that certain herbal extracts can resist natural aging of the skin. For example, because of its romantic and fragrant fragrance, roses have become the favorite fragrance of women in ancient and modern Chinese and foreign fashion. Flower baths adore luxury products. Nearly three-quarters of all perfumes in the world contain the ingredients of rose oil. Among western spices, roses are called the "queen" in flowers. They are the oldest and occupy a considerable natural fragrance. one. It is colorful and charming, sweet and fragrant. It has become the representative offering of Valentine's Day in the West, and it is also the favorite of many women in Chinese and foreign history. British famous Princess Diana's favorite perfume master jean patou made the joy rose perfume in 1929. During the Ptolemy dynasty in ancient Egypt, Queen Cleopatra, known as the Queen of Cleopatra, was fond of the fragrance and aphrodisiac of roses, and took a rose bath every day, even in the bedroom. The ground was covered with 45 cm thick rose petals, and the rich fragrance of roses made Caesar the great and Roman star Anthony intoxicated, and fell down under her pomegranate skirt.

Xiang Fei, also known as Rong Fei in Chinese history, was the only Uighur woman from the 41 concubines of the Qianlong Emperor of the Qing Dynasty. It is said that she never applied fat powder or sachets, but exuded a scent of roses. "Jade Rong Weijin's fragrance is striking and refreshing. It turned out that Xiang Fei was the princess of the Hui Hui chief in Xinjiang. She lived at the foot of the Tianshan Mountains for a long time. She has been eating local special rose sauce since she was a child. She has to eat almost every meal. Even if she is sent to the Royal Palace in Beijing, she has to let her Ren Qianli brought the rose sauce from Xinjiang to eat, and the legend of the rose scent she radiated from her body may be related to this.

Jiang Song Meiling loves roses even more. At the official residence of Shihlin in Taiwan, there is a large specialized rose garden, full of all kinds of roses, and used in rose baths, rose sauces and facial masks, up to 106 years old. Still elegant and luxurious! Become an indicator of modern love roses!

The main chemical components of rose essential oil are citronellol (roseol), geraniol, phenethyl alcohol, acacia alcohol and its esters, linalool, rose ether, eugenol, and methyl eugenol. The amount of these aromatic substances and the differences in their chemical composition have produced a rich, romantic, sweet, mysterious rose fragrance that is loved by women all over the world. It promotes penetration and antioxidant activity of Rhodiola rosea essential oil. Under the action, it is smoothly absorbed by the human body, enters the blood and body fluids, and quickly swims throughout the body with the circulatory system. It may exude a long-lasting romance through the sweat glands in the nose, armpits, umbilicus, groin and around the genitals, as well as small sweat glands around the body The fragrant fragrance of roses helps people cover up their body scent.

In 2008, a preliminary study of "rose biopharmaceutical research" by Li Ming et al. Of Jinan Health School of Shandong Province showed that rose essential oil is a precious industrial raw material extracted from roses and is known as "liquid gold", which is 1.5 times the value of gold. Utilizing rich V _A , V _B , V _C , V _E , V _K and tannic acid (which contains a higher amount of V _C , which is more than 8 times higher than Chinese kiwifruit, can be called the king of vitamin C) Rose essential oil and other beneficial ingredients can prevent skin aging, smooth skin fine lines and adjust skin balance.

In January 2010, Wang Pei, Liaoning University of Traditional Chinese Medicine, and other experimental studies on the effect of resveratrol on H ₂ O ₂ content and ATPase activity in naturally aging mice. The experimental results show that resveratrol can scavenge free radicals and reduce The content of hydrogen peroxide (H ₂ O ₂ ) in the skin tissue and the improvement of the activity of adenosine triphosphate ATPase have been speculated and proved its role in delaying skin aging. In 2010, Liaoning University of Traditional Chinese Medicine Wu Jingdong and others "Experimental study on the effect of resveratrol on T-SOD activity and MDA content in naturally aging mice", the conclusion shows that: resveratrol can increase the total superoxide in the serum of naturally aging mice Dismutase (T-SOD) activity reduces malondialdehyde (MDA) content in skin tissues. Resveratrol has certain control and effect on the skin aging process of mice by removing oxygen free radicals, and can delay its skin aging in a dose-dependent manner. After 2010, Wang Pei, Liaoning University of Traditional Chinese Medicine, Zhang Xiaoli, Liaoning Institute of Health Technology, Zhang Ye, Department of Dermatology, Anorectal Hospital of Liaoning Province, and other different research units each conducted experimental studies on the skin of naturally aged mice with the same resveratrol, and summarized the conclusions. Confirmed: Resveratrol can increase the content of glutathione (GSH) and antioxidant free radicals in mice, reduce the content of hydrogen peroxide (H ₂ O ₂ ) in skin tissues and increase the activity of ATPase, and prevent the skin from naturally The damage of mitochondrial DNA caused by aging reduces the occurrence of mt DNA deletion mutation and achieves the effect of delaying skin tissue aging. In 2011, Zhang Ye of the Department of Dermatology, Anorectal Hospital, Liaoning Province, and others "Experimental study of resveratrol on mitochondrial DNA damage in the skin of naturally aging mice", the conclusion shows that: resveratrol can prevent skin mitochondrial DNA damage caused by natural aging. In April 2011, Liaoning University of Traditional Chinese Medicine Wu Jingdong, Zhang Xiaoli, and other studies on the effect of resveratrol on the changes of GSH and anti-superoxide anion free radical content in aging mice, discussed aging is a normal and complex physiological phenomenon in life. It is a comprehensive process of various biochemical reactions in the body. With the increase of age, the generation of free radicals increases, and the reduction of free radical scavenging related enzyme activities and the decline of the immune function of the body are all important mechanisms of aging. According to research by Hung and others in 2000, resveratrol has stronger antioxidant effects than vitamin E and vitamin C, and can scavenge free radicals, especially hydroxyl free radicals, protect DNA from damage, and inhibit glutathione disulfide. The formation of the peptide puts glutathione in a reduced state, thereby inhibiting the formation of free radicals. Conclusion: Resveratrol can increase the content of GSH and anti-superoxide anion free radicals, improve the body's antioxidant capacity, and effectively delay the aging of skin tissue, and it has no obvious relationship with the dose. In 2011, Zhengzheng Wu of Yichun College of Beauty in Jiangxi Province took "Stone Rhodiola to study the skin of acute aging model mice" and concluded that Rhodiola polysaccharide can significantly increase the hydroxyproline content in the skin tissue of aging mice, thereby promoting skin collagen. Protein synthesis has the effect of enhancing skin elasticity and delaying skin aging. In 2015, Baiyang, Dalian University of Technology, and other studies on "the preparation of licorice flavonoids and their anti-aging effect on skin" concluded that licorice flavonoids significantly inhibited the production of collagen degrading enzymes (mmp ~ L1) in skin cells caused by UVB, and the concentration of licorice flavonoids At 1 and 10 μg / mL, the production of mmp ~ L1 was reduced by 23% and 56%, respectively, indicating that it has anti-aging effects.

Researchers have discovered that certain herbal extracts can resist skin photoaging, such as: "The mechanism of UVA-induced human fibroblasts and keratinocyte damage and the photoprotective effect of resveratrol" by Chen Mingliang of Central South University in China in 2008. The conclusion indicates that resveratrol can repair the damage of human skin fibroblasts and H ₂ C ₂ T cells by long-wave ultraviolet (UVA), and by reducing the expression of iNOS mRNA protein, reducing the synthesis of NO to achieve repair of ultraviolet damage effect. In August 2009, Chen Mingliang from Central South University of China, etc. conducted a study on "Effects of Resveratrol on Skin Fibroblasts Proliferation and Inducible Nitric Oxide Synthase Expression after Long Wave Ultraviolet Radiation", and concluded that long wave ultraviolet (UVA) can inhibit The proliferation of dermal fibroblasts in vitro induces the expression of nitric oxide synthase (iNOS), and resveratrol has a protective effect on fibroblasts damaged by UVA radiation. In September 2009, Jia Lili and other dermatologists from the Affiliated Hospital of China Medical University conducted a study on the "protective effect of resveratrol on skin damage caused by simulated sunlight", and concluded that resveratrol and antioxidants can effectively protect simulated sunlight Acute light damage caused by irradiation. In 2009, Kuang Xiaodong of Nanchang University and other researches on the "protective effect of resveratrol on skin photoaging" concluded that resveratrol can increase the antioxidant enzyme activity of photoaging skin, change the content of oxidation products, and achieve anti-photoaging of skin. effect. A 2011 study by Ye Kan of Suzhou University and other "Effects of Resveratrol on the Life and Reproduction of Nematodes Irradiated by Cobalt 60COγ Rays", the results show that resveratrol can increase the lifespan of irradiated nematodes, increase the survival rate of spawning, and protect them. Mitochondrial function, its mechanism may be related to resveratrol's reduction of mitochondrial damage caused by free radicals. In 2011, Tang Hua of Central South University of China and other studies on the "protective effect of resveratrol on human keratinocytes after irradiation with medium-wave ultraviolet rays", the conclusion showed that different concentrations of resveratrol had no proliferation on normal human keratinocytes (HaCaT). Obviously, resveratrol has a protective effect on the irradiated HaCaT cells in vitro, and the mechanism may be related to increasing the oxidase activity and eliminating free radicals. The protective effect of high-concentration resveratrol is stronger than that of low-concentration, indicating that this protective effect of resveratrol has a certain dose-effect relationship. In 2012, Tianjin Medical University Wang Jingjing and other "protective effects of resveratrol on human keratinocyte damage induced by medium-wave ultraviolet rays", the conclusion showed that resveratrol can significantly inhibit MMP-1 produced by HaCaT cells induced by UVB irradiation. The changes of MMP-9 and TIMP-1 mRNA content can protect the skin from photoaging. In 2012, Xi'an Jiaotong University Zheng Wen et al. "Effects of resveratrol on the levels of MMP-1 and apoptosis in human skin fibroblasts exposed to UVB". The conclusions indicate that resveratrol can inhibit UVB-induced human skin Elevated levels of MMP-1 in fibroblasts can reduce cell apoptosis due to UVB radiation. Resveratrol may protect the skin from ultraviolet radiation through this mechanism. In 2012, Yan Yan from China Medical University of China and other researches "Suppressive effect of resveratrol external application on skin erythema caused by simulated sunlight", the conclusion shows that external application of resveratrol can effectively prevent and treat skin erythema induced by simulated sunlight , Especially for preventive applications. In 2010, Xie Wenhao of Fu Jen University in Taiwan conducted a study on "Application of Glycyrrhetinic Acid Derivatives to Skin Physiology". The conclusion showed that licorice can be used as a compound to improve the performance of AQP ~ 3 (AQP ~ 3 type 3 water molecular channel is a small Molecules (~ 30KDa) and "aquaporins" embedded in human epidermal cell membranes, their functions can promote the transport of water and small molecules such as glycerin. Many studies have pointed out that improving AQP ~ 3 performance will increase cell proliferation and cell migration, (Strengthen the skin barrier to promote skin wound healing) Applied to skin with AQP ~ 3 protein deficiency and other related diseases to delay skin aging. In 2012, Tang Hua et al. Of the Department of Dermatology, Changsha People's Hospital, etc., studied the effect of resveratrol on the expression of AQP3 (aquaporin) in human keratinocytes after UV irradiation, and concluded that resveratrol may inhibit AQP3 mRNA caused by UVB. Decrease and increase oxidase activity, scavenge free radicals, thereby delaying skin aging. In 2014, Guangzhou Qing Institute of Dermatology, etc. "Resveratrol as a NrF2 (nuclear factor E2-related factor 2) activator to prevent UV-induced skin oxidative stress damage", the conclusion states that resveratrol activates NrF2 The mechanism may be to reduce the expression of Keap1 and promote the accumulation of NrF2 in the nucleus, thereby protecting HaCaT cells from oxidative stress caused by UVA irradiation. In 2014, Tianjin Key Laboratory of Molecular Nuclear Medicine, Wang Yan and others, "Resveratrol inhibits radiation-induced NLRP3 expression", and the conclusion indicates that resveratrol can significantly inhibit radiation-induced 1L ~ 1β and NLRP3 transcription and expression, and May play a role through sirt1 ~ NLRP3 pathway. In 2015, Lei Wei of the Department of Dermatology, the First Affiliated Hospital of China Medical University, and other studies on the "photoprotective effect of resveratrol on human fibroblasts exposed to UVA", the conclusion shows that 0.01mmol / L resveratrol may inhibit 1L ~ 1β And NF ~ kB and its expression of MMPs, thereby inhibiting the photodamage of human fibroblasts caused by UVA irradiation. From 2007 to 2010, different academic research units, such as Zhang Yashu, Liu Rongxiu, and the Nanjing Medical University, and Shen Fei, of the Institute of Cosmetics, Chianan University of Pharmacy, Taiwan, and Rhododendron studied the effects of photoaging on human skin. Comprehensive conclusions confirm that: Rhodiola really protects and repairs the photoaging defects of human skin caused by UVB / UVA irradiation, and its mechanism is that Rhodiola can improve the expression of type 1 procollagen protein caused by UV irradiation; And the increase in the expression of MMP ~ 1 protein, and can increase the antioxidant enzyme activity in human keratinocytes, inhibit the lipid oxidation reaction to achieve the effect of scavenging free radicals, significantly increase the content of hydroxyproline in skin tissue, and then maintain the extracellular matrix The collagen content enhances skin elasticity. From 2010 to 2015, different academic and clinical units such as Chen Dongbo of Guangdong College of Medicine, Tian Zhuohua of Lanzhou University, Bian Fang, Zhao Min, and Yang Guilan of the Department of Dermatology of General Hospital of Lanzhou Military Region jointly worked on the effects of licorice, Rhodiola, Scutellariae on human skin photoaging. The research and comprehensive conclusions confirm that: Licorice, Rhodiola and Scutellariae indeed inhibit photoprotection and repair of human skin caused by medium-wave ultraviolet (UVB) irradiation, and their protective mechanisms are licorice and red Sedum and Scutellariae can induce the expression of caspase ~ 8, HSP27, HSP70 in skin tissues and up-regulate the expression of IL ~ 10, SOD, NO, and inhibit the production of MDA, TNF ~ α, enhance the skin's antioxidant capacity, and promote the elimination of oxygen free radicals And skin collagen synthesis, reduce skin cell apoptosis.

Researchers have discovered that certain herbal extracts can promote skin whitening and spotting effects, such as: "Research on the whitening properties of resveratrol" by Zhang Tingyi, Cosmetic Research Institute, Hongguang University of Science and Technology, Taiwan in 2009. The conclusion states that resveratrol can Effectively inhibits the enzyme activity of mushroom tyrosinase, and from the results of enzyme kinetic analysis, it is speculated that resveratrol should be a non-competitive inhibitor of tyrosinase. The addition of resveratrol and gallic acid has a multiplier effect on its ability to inhibit extracellular tyrosinase activity. Conducive to the development of skin whitening care products. In 2011, Yang Yang of Beijing Forestry University and others "Research on the kinetics of tyrosinase inhibitory activity of resveratrol in vitro", the conclusion shows that resveratrol can inhibit tyrosinase monophenolase and diphenolase, It can be used not only for the clinical treatment of hyperpigmented skin diseases (chloasma, freckles, etc.), but also for cosmetics to brighten the complexion, and it can also be used as a food additive to prevent the browning of fruits and vegetables and to treat various pigment diseases. . In 2011, Shi Xianmin, School of Life Sciences, Tsinghua University, Beijing, etc., “Preliminary research on the resveratrol whitening effect” concluded that resveratrol does have a certain inhibitory effect on the proliferation of B16 melanocytes and intracellular tyrosinase. It reduces the synthesis of melanin and has a whitening effect. Adding resveratrol as a whitening agent to cosmetics provides an experimental basis. In 2011, Beijing University of Traditional Chinese Medicine Liu Rong and other "Animal Evaluation and Application Research on Whitening Effectiveness of Cosmetic Raw Materials", the conclusion states that resveratrol has a significant inhibitory effect on melanin production, indicating that resveratrol can not only effectively inhibit tyrosinase activity , Has the potential to inhibit melanin production, and has strong transdermal and cell membrane functions, and has a better whitening effect. In 2013, Guangdong Inspection and Quarantine Technology Center, Qin Yao and other "Animal Models to Evaluate the Whitening Effect of Cosmetic Raw Materials", the conclusion indicates that the combined use of arbutin / resveratrol can significantly improve the whitening effect, and the formation and distribution of melanin particles in tissues obviously decrease. Guangdong Medicine in 2014 The experimental study of "Resveratrol's inhibition of tyrosinase activity" by Luo Liqiong of the hospital shows that resveratrol can reduce the synthesis of melanin in human skin, thereby achieving whitening, treating melasma, and freckles. Under optimal conditions, resveratrol has a significant inhibitory effect on tyrosinase. With the increase of the concentration of the effector, resveratrol, the activity of tyrosinase gradually decreases, and its half inhibition rate IC50 is 5.3mmol / Lo can theoretically show that resveratrol is used in cosmetic additives to increase the whitening effect of cosmetics. From 2003 to 2009, Ma Jingbo of Shanghai Fudan University and Zhang Yashu of the Cosmetic Research Institute of Taiwan ’s Jianan Pharmacology University; and Taiwan ’s China Medical University Jian Yinzhi and other scientific research units studied the effects of Rhodiola on human skin whitening effects. The comprehensive conclusions are all Confirmed: Rhodiola can inhibit tyrosinase activity and reduce melanin content. The mechanism may be that it inhibits melanin cell synthesis by regulating the expression of tyrosinase mRNA and protein, which proves that Rhodiola treatment of skin pigmentation diseases is Safety and effectiveness, suitable for screening raw materials for whitening skin care products. In 2014, Wang Xiaowen of China Medical University in Taiwan conducted a study on "Rose Extracts Inhibit Melanin Production and Its Mechanism". The conclusion shows that rose extracts have a significant inhibitory effect on tyrosinase and inhibit melanin production by melanocytes. , Has a whitening effect, is a very good cosmetic additive. From 2010 to 2013, Chen Huichun and Chen Shilong of Jianan Pharmaceutical University of Science and Technology of Taiwan respectively took "the formula design of licorice in whitening cosmetics" and "the research on the development of licorice extract and its skin efficacy". The conclusions indicate that the licorice extract is indeed It has excellent antioxidant and whitening activity, has excellent safety and stability, has an improvement effect on UV stains, and has the potential to become an excellent functional raw material for skin care products. From 2011 to 2015, Yue Xue of Nanjing Medical University; Yinglong of Baicheng Medical College of Jilin Province; Wang Yan of Tianjin University of Traditional Chinese Medicine; Xunwen of Hainan Dermatological Hospital and other medical research units. ： Glycyrrhizin in licorice is currently recognized as the most effective natural whitening agent in the world. It can inhibit both tyrosinase activity and dopa pigment interconversion and dihydroxyindolecarboxylic acid (DHICA) oxidase. Activity, which can inhibit the migration of melanocytes by reducing the concentration of free calcium ions in melanin cells. Whitening, brightening, and spotting effects are related to the ability to scavenge free radicals similar to SOD (peroxide dismutase) and the antioxidant free radicals similar to vitamin E, and can reduce the formation of melanocytes.

Researchers have found that certain herbal extracts can help skin damage / wound healing. For example, in 2004, Liu Dewu, Department of Pharmacy, Burn Center, First Affiliated Hospital of Jiangxi Medical College, etc., used "Scutellaria Baicalensis to induce human epidermal stem cell proliferation to construct tissue-engineered skin to treat skin." "Defective medical test" study, the conclusion shows that: Scutellaria baicalensis can promote the growth of human epidermis in vitro, can induce the proliferation of epidermal stem cells, and can repair the structure and function of skin defects. A 2011 study by Zhang Xi and others from Shanghai Ocean University on the effect of traditional Chinese medicine on wound healing effect and pearl volume in pearl nucleus surgery in Hepu, concluded that resveratrol or Yunnan Baiyao had the best effect on a single wound treatment. It can significantly promote the expression of basic fibroblast growth factor (bEGF) and vascular endothelial growth factor (VEGF) in the body, thereby accelerating the growth of blood vessels and facilitating wound healing. In 2012, Gao Yici of Taiwan University of Science and Technology, "Comparing the Benefits of Biocompatible Bacterial Cellulose and Collagen I Membrane Combined with Resveratrol for Wound Repair" Master Thesis Research, Conclusions: According to the experimental results, the use of resveratrol Because of its good biocompatibility and regulation of responsiveness, bacterial fibrin can provide a better environment for damaged skin for skin repair. In 2009, a study on "Exploration of Rhodiola Extract in the Animal Model of Burns and Scald Rats" by Cai Xingjin, Institute of Cosmetic Science and Technology, Jianan Pharmaceutical University, Taiwan, concluded that salidroside may reduce lipid peroxidation and increase the body Total anti-oxidation and reduce inflammatory factors (TNF ~ αIL ~ 1β) in the skin to achieve the repair effect caused by burn injury. From 2008 to 2010, Guangzhou South Medical University Zheng Gaoyun, Zhou Yunfeng, etc. studied the effect of ecdysterone on the healing of experimental skin trauma and defects in rabbits and its mechanism. The comprehensive conclusions indicate that ecdysterone can indeed promote the healing of experimental skin trauma in rabbits. The mechanism may be related to the induction of high expression of epidermal growth factor receptor (EGFR) and bFGF. In 2010, a study on "Application and Evaluation of Electrospun Gelatin Nanofibers and Centella Asiatica in Skin Tissue Engineering" conducted by Ye Renhe, Chung-tai University of Science and Technology, Taiwan, concluded that Asiaticoside is slowly released into the injured skin tissue and promotes internal Fibroblast proliferation has the effect of promoting wound healing and shortening the healing time. 2007 ~ 2014 ASRock of Zhejiang People's Hospital; and Tian Peng of Beijing Jishuitan Hospital The medical research on the effects of alginate (also known as sodium alginate and alginate) on refractory skin defect wounds and the clinical effect of wound healing on scar skin donor area, the conclusion shows that alginate can treat refractory Skin defect wounds can not only increase the expression of basic fibroblast growth factor (bFGF) and the proliferation of epidermal stem cells, but also promote the rapid and high-quality healing of wounds through the interaction of bFGF and epidermal stem cells. In 2015, Wu Jun of the University of Manitoba, Canada, and other studies on the role of alginate in promoting the healing of wounds in mice with full-thickness wounds. The conclusion indicates that alginate can be used in skin wounds to promote bone marrow mesenchymal stem cells (MSCs). ) Adheres, grows, differentiates, and migrates in the body to promote skin tissue repair and accelerate wound healing.

Researchers have found that certain herb extracts can help any skin flap survive. For example, in 2009, Dong Yuanmei of Xijing Hospital and others took the "Experimental Study on the Effect of Resveratrol on Arbitrary Flaps Transplanted", and concluded that resveratrol can significantly Reduction of malondialdehyde (MDA) content and inhibition of myeloperoxidase activity after any flap transplantation, verified the anti-oxidant and anti-free radical effects of resveratrol, thereby reducing capillary endothelium in flap tissue Cell damage, reduce adhesion of endothelial cells and leukocytes after ischemia-reperfusion injury, and effectively prevent free radical damage during ischemia-reperfusion. In addition, resveratrol can effectively inhibit platelet aggregation, thereby reducing the damage to the microcirculation of the flap. Because resveratrol can reduce the ischemia-reperfusion injury of the flap, resveratrol can improve the ischemic flap. Potential for survival.

Researchers have discovered that certain herbal extracts can help to wrinkle / reduce skin scars. For example, in 2007, Zhang Yuanhao's research team at Stanford University in the United States discovered in a mouse experiment that as long as a single gene called "NFkB" is blocked, Let the mice's aging skin return to youth after two weeks of treatment. Dr. Nicholas Perricone, a well-known global authority in aesthetic medicine, said that when skin cells are in a highly activated state of NFkB and accompanied by other AP-1 transcription factors, skin wrinkles will occur. Many studies have proven that resveratrol is the natural nemesis of NFkB. Dr. Zhang Yuanhao managed to control NFkB in the skin cells of some two-year-old mice (equivalent to human half a hundred years old). Applying a specific cream on the mouse's skin will stop the skin cells. Aging, after two weeks, the genes and tissues of the skin of the treated part of the mouse became younger! On December 1, 2007, the China Times and Lianhe Daily in Taiwan reprinted this research result. In 2007, Moreau used Sirt ~ 1 activators (such as resveratrol and Kluyveromyces amphoteric peptides) once a day for four weeks on the face and neck skin of 33 women aged 37-64 years. The results show that Sirt ~ 1 activator can increase the expression of Sirt ~ 1 in human normal skin fibroblasts and epidermal cells, and can reduce skin cell aging and DNA damage caused by medium-wave ultraviolet (UVB). Studies have shown that after the first application of Sirt ~ 1 activator, the skin hydration is significantly improved, and skin aging problems such as skin wrinkles and pigmentation are significantly improved. In September 2005, Zhu Qian of Shenzhen Second People's Hospital and other "Effects of Resveratrol on Collagen Synthesis and Related Gene Expression" research, the conclusions indicate that resveratrol can inhibit the proliferation of human skin scar tissue fibroblasts. And it was dose / time dependent. In addition, resveratrol has a significant inhibitory effect on type I and type III procollagen mRNA, and the inhibitory effect on type I procollagen mRNA is stronger than that of type III procollagen. (One of the main characteristics of hypertrophic scars is excessive collagen deposition. The collagen of human skin and scars is mainly composed of type I and type III.) Many reports have shown that collagen hyperplasia of hypertrophic scars is mainly type I, that is, type I. The increase of collagen was more obvious than that of type III. Therefore, the molecular mechanism of resveratrol's inhibitory effect on the synthesis of type I and III procollagen may be to down-regulate the expression of collagen type I and type III mRNA from the level of transcription, thereby inhibiting the synthesis of collagen by fibroblasts and reducing the degradation of collagenase. In March 2006, Zhu Qianfang and the Chinese Journal of Natural Medicine published a paper entitled "Effects of Resveratrol on Human Skin Fibroblasts and Hypertrophic Scars in Rabbit Ears". The conclusions indicate that: (1) Resveratrol reduces fibroblasts Proliferation index is one of the ways to inhibit scar hyperplasia, and it is dose / time dependent. (2) As the resveratrol concentration increases, the rabbit ear scar hypertrophy index decreases, which is consistent with the in vitro experiments of scars. In July 2006, Zhu Qian of Shenzhen Second People's Hospital and others in a clinical article entitled "Effects of resveratrol on the shrinkage characteristics of three-dimensional cultured human skin scar fibroblasts", confirming that resveratrol has proliferating fibroblasts in human skin scar tissue. And type I and III procollagen mRNA has a significant inhibitory effect, and can reduce fibroblast proliferation index and rabbit ear scar hyperplasia index. The conclusion shows that resveratrol can indeed promote the contraction of skin scar fibroblasts cultured in three dimensions. In 2009, Xi Zhanrong, Zhuhai Second People's Hospital of Guangdong, and other studies on the "Effects of Resveratrol on Atrial Natriuretic Peptide and Brain Natriuretic Peptide from Fibroblasts", the conclusion showed that the scar fibroblasts were found in the experiment. Atrial natriuretic peptide and brain natriuretic peptide are secreted during external culture. Resveratrol can significantly inhibit atrial natriuretic peptide and brain natriuretic peptide secretion, which indicates that resveratrol has a certain inhibitory effect on the proliferation and hypertrophy of fibroblasts, which may be related to Up-regulated NO-cGMP signal pathway is related.

Researchers have found that certain herbal extracts and chemical ingredients have a percutaneous penetration / moisturizing effect, such as: "Study on the Transdermal Delivery and Delivery System of Natural Antioxidant Resveratrol" by Huang Zirou of Chang Gung University in Taiwan in 2008. It shows that resveratrol has better skin penetration function in non-ionic state than in ionic state. In 2011, Beijing University of Traditional Chinese Medicine Liu Rong and other "Animal Evaluation and Application of Cosmetic Raw Materials for Whitening Effectiveness" research, the conclusion is that resveratrol has a stronger transdermal and transmembrane function. In 2011, Tian Hui of Tianjin University of Traditional Chinese Medicine and other "in vitro transdermal study of resveratrol phospholipid complex", the conclusion shows that: resveratrol phospholipid complex improves the hydrophilicity and lipophilicity of resveratrol, and cumulative penetration Significant increase, the release behavior is in line with the first-level release model. Its mechanism of promoting penetration may be related to affecting the structure of the stratum corneum and the fluidity of the lipid layer. 2012 Chinese invention patent The inventor ZL2012 10191413.3 and the United States also passed the invention patent "Food Formula for Nutrient Maintenance and Repair of Various Stem Cells and Its Manufacturing Method", stating that sodium alginate (also known as alginate) and hydroxypropyl ~ β ~ Cyclodextrin-embedded resveratrol is used as a carrier for the inclusion compound, which can make the "formula" achieve the "long residence time" in the body and reach the "target" controlled release effect. In 2016, raw material suppliers have shown that hydroxypropyl β-cyclodextrin is used as a stabilizer, emulsifier, and deodorant in the field of cosmetic raw materials, which can reduce the irritation of skin and mucosal tissues by organic molecules in cosmetics and enhance the stability of active ingredients. To prevent the volatilization and oxidation of nutrient components, and to increase the water-solubility of insoluble flavors and fragrances, to maintain a slow and long-lasting flavor. In 2007, ASRock of Zhejiang People's Hospital and other researches on “Alginate dressing and freeze-dried rat epidermal growth factor (mEGF) proliferation of epidermal stem cells in refractory wounds”, the conclusion shows that alginate has extremely strong absorbability and can absorb the equivalent of A liquid that is 20 times its own weight is often used to release the drug into the body by embedding the drug. If it is liquid, it can effectively control the leakage and prolong the use time to maintain a humid (moisturizing) environment. In 2013, Chengyang Wu University of Taiwan ’s Wu Chengying ’s “Rose petal extract antioxidant and whitening effect” study, the conclusion shows that rose essential oil can be found at high concentrations (500 μg / mL and 1000 μg / mL). It has significant inhibition of melanin production by B16 melanocytes. The crude polysaccharides extracted from rose petals are similar to hyaluronic acid in the evaluation of hygroscopicity and moisturizing ability, that is, the rose polysaccharides extracted from roses have the potential to develop into natural moisturizing ingredients and cosmetic raw materials. In 2014, the clinical research on “Moisturizing properties and elasticity of mulberry leaf extract on the skin”, such as Tian Furong, Zhejiang Yiwu Entry-Exit Inspection and Quarantine Bureau, concluded that the mulberry leaf extract has obvious skin moisturizing and moisturizing functions, and improves skin elasticity, including Improved coarse elasticity, net elasticity and firmness, and anti-wrinkle effect. A study on the effect of Rhodiola alpine volatile oil on transdermal absorption of xanthoside in vitro in Dafeng Tiansheng Pharmaceutical Co., Ltd. in Jiangsu Province in 2014. The conclusion shows that: 1% Rhodiola rosea volatile oil combined with 3% Azone It has the best effect on the penetration promotion of flavoside (usually 1% of Rhodiola rosea, 5% of Azone) means good transdermal effect, which proves the transdermal effect of Rhodiola rosea. In 2015, Yan Yan of Tianjin University of Traditional Chinese Medicine and other "effects of penetration enhancer on the transdermal penetration effect of Glycyrrhizin in vitro", the conclusion shows that: Glycyrrhizin has a low transdermal absorption rate, and the penetration enhancer can effectively make it penetrate the skin. Different penetration enhancers can promote the transdermal penetration of Glycyrrhizin, and the best is Azone.

The present inventor has taken into account the above-mentioned drug experimental reports that some Chinese herbal medicines are available to increase the life of human epidermal stem cells. However, at present, they have only been applied to the research of wound healing such as burns, traumas, and so on. And some of these Chinese herbs are not transdermal, it is difficult to penetrate into the body to proliferate human epidermal stem cells, and the characteristics of stem cells are "proliferative" and "differentiation" exactly the same as those of cancer stem cells, so they stimulate normal people Epidermal stem cells may also trigger concerns about skin cancer stem cells during incubation period! There are practical difficulties and lack of improvement! I hope to provide a breakthrough design to enhance the practical effect. After intensive research and design, we have gathered many years of experience in stem cell preservation banking and have obtained Chinese and US invention patents: food for nutrition and maintenance of a variety of stem cells. The formula and its manufacturing method are honored, and the accumulated professional technical knowledge and practical experience of related product design, production and sales The design is verified, and the epidermal stem cells for skin care, the Chinese herbal medicine composition with multiple functions and its mask have been developed to provide leading cutting-edge products of truly practical mask technology for both men and women.

Therefore, the object of the present invention is to provide an epidermal stem cell for skin care, a Chinese herbal medicine composition with multiple functions and a mask thereof to improve various problems of the skin.

The technical means adopted by the present invention to solve the problems of the conventional technology is to provide a Chinese herbal medicine composition containing the following raw materials in weight percentages: grape skin or Polygonum cuspidatum root extract 5% to 11%; mulberry leaf extract 0.5% to 1.1% ; Scutellaria baicalensis extract 5% ~ 11%; Tripterygium root extract 0.7% ~ 1.3%; Brown algae extract 3% ~ 9%; Rose extract 1% ~ 5%; Rhodiola rosea extract 1% ~ 5%; Licorice extract 0.5% to 6.5%; and additives 51% to 83%, wherein the grape skin or Polygonum cuspidatum extract includes not less than 770mg / L of resveratrol, and the mulberry leaf extract includes not less than 50mg / L of ecdysterone, the Scutellaria baicalensis extract includes not less than 770mg / L baicalin, the tripterygium root extract includes not less than 70mg / L asiaticoside, and the brown algae extract includes not less than 570mg / L of alginate, the Rhodiola rosea extract includes not less than 70mg / L of Rhodiola rosea essential oil and 170mg / L of salidroside, the licorice extract includes not less than 170mg / L Glycyrrhiza flavonoids and Glycyrrhizin not less than 120 mg / L, and the rose extract includes rose leaf stem cells not less than 170 mg / L and rose essential oil not less than 70 mg / L.

In one embodiment of the present invention, a Chinese herbal medicine composition is provided. The grape skin or Polygonum cuspidatum extract includes 770-830 mg / L of resveratrol, and the mulberry leaf extract includes 50-110 mg / L of ecdysterone. The Scutellaria baicalensis extract includes 770-830mg / L baicalin, the tripterygium root extract includes 70-130mg / L asiaticoside, the brown algae extract includes 570-630mg / L alginate, and the red Sedum extract includes 70-130 mg / L of Rhodiola rosea essential oil and 170-230 mg / L of salidroside. The licorice extract includes 170-230 mg / L of licorice flavonoids and 120-180 mg / L of licorice. And the rose extract includes 170 ~ 230mg / L rose leaf stem cells and 70 ~ 130mg / L rose essential oil.

In one embodiment of the present invention, a Chinese herbal medicine composition is provided. The grape skin or Polygonum cuspidatum extract includes 800 mg / L of resveratrol, the mulberry leaf extract includes 80 mg / L of ecdysterone, and the yellow The tincture extract includes 800 mg / L baicalin, the tripterygium root extract includes 100 mg / L asiaticoside, the brown algae extract includes 600 mg / L alginate, and the rhodiola extract includes 100 mg / L of Rhodiola rosea oil and 200 mg / L salidroside. The licorice extract includes 200 mg / L of licorice flavone and 150 mg / L of glycyrrhizin and the rose extract includes 200 mg / L of rose leaf stem cells and 100 mg / L. Rose essential oil.

In one embodiment of the present invention, a Chinese herbal medicine composition is provided. The additive includes a wetting agent, a thickener, a preservative, a solubilizer, a lubricant, a plasticizer, an emulsifier, a surface coating agent, a dispersant, and a flavor. One or more of a group consisting of an agent, an acidity regulator, and a carrier.

In one embodiment of the present invention, a Chinese herbal medicine composition is provided, and the wetting agent is one or more selected from the group consisting of sodium hyaluronate, hyaluronic acid, urea, lactic acid, lactate, and glycerol.

In one embodiment of the present invention, a Chinese herbal medicine composition is provided. The weight percentage of the sodium hyaluronate is 2% to 8%.

According to an embodiment of the present invention, a Chinese herbal medicine composition is provided, and the carrier includes a soybean extract, a potato extract, and an azatone.

In one embodiment of the present invention, a Chinese herbal medicine composition is provided. The weight percentage of the soybean extract is 5% to 11%, the weight percentage of the potato extract is 7% to 13%, and the weight of the azotonone. The percentage is 1% ~ 5%.

In one embodiment of the present invention, a Chinese herbal medicine composition is provided. The 5% to 11% soybean extract includes not less than 770 mg / L of soybean lecithin and the 7% to 13% potato extract includes not less than Hydroxypropyl-β-cyclodextrin at 970 mg / L.

In one embodiment of the present invention, a Chinese herbal medicine composition is provided, comprising: a film-shaped body having a plurality of openings corresponding to eyes, nose and mouth of a human face; and a dressing layer provided on the film-shaped body On the surface, the dressing layer is provided with the Chinese herbal medicine composition according to any one of claims 1 to 8.

The epidermal stem cells for maintaining skin, and the Chinese herbal medicine composition and its mask having multiple functions can have the following advantages: The main purpose of the present invention is to increase the effective molecules of human epidermal stem cells by compound symbiosis and Features of effective dose combination, especially the combination of ingredients that can be combined to multiply the effects and transdermal infiltration can achieve the expected growth of human epidermal stem cells and inhibit the cancer stem cells. In addition to the combination of ingredients, the raw materials of various compositions have the previous The experimental results are used as the basis for scientific basis, and then become the creative new mask formula combination and manufacturing method. Different from the previous technology and use, it can more effectively and quickly increase the life of human epidermal stem cells, play a role in complementing each other, Multi-effects make up for the shortcomings of the previous technology in its use! Become the most characteristic and multifunctional and maintain "human epidermal stem cells", and also have the ability to inhibit the skin cancer cells and increase the vitality of the repair and nourish and regulate the human epidermal stem cells.

The main molecules of the formula for maintaining skin's epidermal stem cells and a Chinese herbal medicine composition with multiple functions, the effective ingredients of which are resveratrol extracted from grape skin / Knots root, ecdysterone extracted from mulberry leaves, Scutellaria baicalin extracted from Scutellaria baicalensis, Asiaticoside extracted from Tripterygium wilfordii, alginate (also known as alginate, sodium alginate) extracted from brown algae, of which resveratrol was prepared by Li Yongjun of Hebei Medical University in 2011, etc. An experimental study on "resveratrol's antibacterial activity against dermatophytes" shows that resveratrol has a strong inhibitory effect on wool-like microsporum, trichophyton rubrum, gypsum trichophyton, and flocculent epidermis Bacterial effect.

Resveratrol made a splash around the world in 2012 by the Taipei Rongmin Institute Qiu Shihua and other news that can suppress cancer stem cells. Earlier in 1997, Professor John Pezzuto of the University of Illinois at Chicago, USA, published Resveratrol in the internationally renowned "Science" magazine. Resveratrol is a powerful anti-cancer mutation that can effectively inhibit the cell processes in all cancer processes, that is, the three main stages of tumor initiation, promotion, and expansion. Resveratrol Can have a suppressive effect. It also shows that the animal test mode of mouse skin cancer shows the effect of suppressing tumor formation. Mice with skin cancer are fed with resveratrol supplement. After 18 weeks of treatment, the number of skin tumors in the body was 98% less than that of mice without resveratrol, which turned malignant cells back into normal cells.

Kundu et al. In a study on resveratrol inhibiting skin cancer in mice, found that resveratrol can inhibit the degradation of IkB, so that the form of NFkB and IkB combined remains in the cytoplasm, and cannot affect the transcription of genes in the nucleus. Induction of tumor cell apoptosis. Kundu et al. Also found that inhibiting the expression of COX-2 in mouse skin tissue to block the activation of MAPKs and AP-1 may be one of the important mechanisms of resveratrol against skin cancer. Roy P et al. Found in mice experiments that resveratrol can regulate PI ₃ K / AKT signaling pathway and inhibit mouse skin cancer.

In 2011, Beijing Capital Medical University Zhao Dongzhi and other "Resveratrol-induced apoptosis pathways in human skin cancer cell lines" research, the conclusion shows that: Resveratrol may first phosphorylate JNK protein, enter the nucleus, and exert an inhibitory effect on Bcl- 2 protein expression, up-regulates Bax and caspase-3 protein expression, and then causes apoptosis.

In 2009, Jiao Huiqin and others in the Capital Medical University of Mainland China conducted an experimental study on "resveratrol inhibits the growth of human skin cancer cell line A431" and concluded that resveratrol has a certain inhibitory effect on the proliferation of skin cancer cell line A431, and Adverse reactions were weaker than the chemotherapeutic drug cisplatin (DDP). Resveratrol can induce apoptosis of A431, which may be related to the inhibition of Bcl-2 expression and the promotion of Caspase-3 expression.

Hao et al. Found that resveratrol can inhibit the growth of human skin squamous cell carcinoma A431 cell lines in nude mice transplanted tumors. One of the mechanisms may be related to activating the MAPK / ERK signaling pathway in tumor tissues, and then activating P53 to induce tumors. Related to apoptosis.

In 2010, Lili Jia and other researchers from China Medical University in Shenyang et al. "The effect of resveratrol on melanin and Langerhans cells after simulated sunlight exposure". The conclusion showed that resveratrol is likely to be a competitive inhibitor of protein substrates. Both cis- and trans-structures have significant tyrosine protein kinase inhibitory activity, thereby inhibiting melanin synthesis and preventing skin darkening. Langerhans cells are "antigen-presenting cells" in the epidermis and play a key role in the skin's immune response. A large number of studies have confirmed that ultraviolet radiation can Causes reduction or failure of intra-epidermal immune presenting cells (Langerhans), which leads to immunosuppression and even skin cancer, and resveratrol combined with antioxidants or used alone can effectively protect against simulated sunlight The increase of melanin particles and the decrease of Langerhans cells caused by irradiation can achieve the effects of whitening and preventing skin cancer.

In 2011, Zhai Xiaoxiang and other researchers from Xuzhou Chinese Medicine Hospital of Jiangsu Province studied the effect of resveratrol on telomerase activity and proliferation and apoptosis of human epidermal-like cancer cells. The conclusion shows that resveratrol can inhibit human epidermal-like cancer cell A431. The mechanism of the proliferation and induction of apoptosis may be related to inhibiting the transcription of telomerase hTERT mRNA gene protein and reducing its protein expression, thereby reducing telomerase activity. (Telomerase activity is abnormally expressed in most tumor cells, while telomerase activity is not detected in normal human cells. Telomerase activation is one of the key links in the development of malignant tumors).

In 2011, Luo Peiyi and others from Guangzhou Women's and Children's Medical Center conducted a "proteomics study of resveratrol-induced apoptosis of human skin squamous cell carcinoma" and concluded that a total of 11 differential protein molecules regulated by resveratrol were identified. Including BAG1, PDCD11, BCLAF1, HSPA9, YWHAZ and other 5 protein molecules that have been confirmed to be closely related to apoptosis, providing a basis and clues for the in-depth understanding of the molecular mechanism of resveratrol against tumors.

Johnson et al. Reported that resveratrol can inhibit melanoma cell-dependent transcription of STAT3 and NFkB, and down-regulate the expression of protein-like interleukin-1 converting enzyme inhibitory protein (cFLIP) and Bc1-xL in cell-type Fas-related death regions. Eventually activate the MAPK and ATM-Chk2-P53 signaling pathways and initiate the apoptotic program.

In 2005 Yangs in vitro experiments found that resveratrol can inhibit the synthesis of oxygen free radicals and inhibit the proliferation of melanoma cells.

In 2008, Zhao Jing of the Center for Disease Control and Prevention of Nantong City, Jiangsu Province, and other authors published a study on "Effects of Resveratrol on the Proliferation and Apoptosis of Human A375 and Mouse B16F10 Melanoma Cells". Resveratrol significantly inhibited the proliferation of human A375 and mouse B16F10 cells in a dose-effect-dependent relationship. The conclusion showed that resveratrol could inhibit the proliferation of malignant melanoma (MM) cells in vitro.

In June 2009, Gao Guihua, et al., Funing County Centers for Disease Control, Jiangsu Province, China, etc., in "In vitro and in vivo studies on the inhibitory effect of resveratrol on the growth of malignant melanoma", the conclusion states that in vitro and in vivo experiments confirm that resveratrol is effective Inhibited the growth of malignant melanoma, and found that its antitumor mechanism may be achieved by inhibiting the expression of p-Akt protein.

A 2010 study by Liu Jingjing of Guangzhou University of Traditional Chinese Medicine on "Synergistic effect of resveratrol on suicide gene therapy for malignant melanoma in mice", the conclusion indicates that in vitro experiments found that resveratrol has a growth inhibitory effect on B16 cells, and it has a significant effect on B16. The cell line tk / GCV system (suicide gene) has a synergistic effect, which is related to the gap junction. In vivo experiments have found that resveratrol also has a synergistic effect on suicide gene therapy of malignant melanoma in mice.

In 2011, Ningxia University Li Yong et al. "Resveratrol inhibits the growth of melanoma cancer cell A375" and concluded that resveratrol has a significant inhibitory effect on the proliferation of melanoma cancer cell A375, and resveratrol significantly inhibits A375 cells. The blocking effect in G1 and S phases has certain concentration-dependent and time-dependent effects, which inhibits its growth and induces apoptosis. In the experiment, typical apoptotic cell morphological characteristics of A375 cells were observed, indicating that resveratrol has a significant effect on melanin. The growth of cancer cells has a significant inhibitory effect.

In 2011, Zhou Feihong of Wuhan First Hospital et al. "Effects of resveratrol on the expression of angiotensin II and its receptor in A375 human melanoma cell line", the conclusion shows that resveratrol and local kidney of malignant melanoma And regulation of angiotensin system. Its regulatory mechanism is not achieved by its angiotensin-converting enzyme inhibitor (ACEI) effect, but by inhibiting the expression of ATIR.

In 2013, Yan Yuehua from Zhongnan Hospital of Wuhan University and other studies on the "Effects of Resveratrol on the Proliferation and Apoptosis of Human Malignant Melanoma Cell A375", the conclusion showed that: Resveratrol at a certain concentration had a significant inhibition on human melanoma A375 cells. Proliferative effect, mainly inhibits the proliferation of A375 cells by inducing apoptosis (early apoptosis).

In 2015, Ren Jianwen, Department of Dermatology, Second Affiliated Hospital of Xi'an Jiaotong University, and other studies on "Effects of Resveratrol on Hypoxia-Induced Skin Melanoma Cell A375 Invasion and Interstitial Transformation of Epithelial Cells", the conclusion stated that resveratrol can Inhibition of hypoxia-induced proliferation and migration of melanoma cells A375 may be related to inhibition of hypoxia-induced HIF ~ 1α protein (hypoxia-inducible factor) up-regulation and epithelial-mesenchymal transition (EMT) processes. Resveratrol may act as a HIF ~ 1α inhibitor and become a candidate drug for the prevention and treatment of melanoma.

In 2012, Meng Deping and others from Shandong Academy of Medical Sciences conducted a study on "Resveratrol's Inhibitory Effect on Melanoma Cell Proliferation" and concluded that resveratrol can inhibit the proliferation of WM239 cells, causing changes in the cell cycle and a marked increase in apoptotic cells.

Resveratrol is indeed a dual-acting biomedical molecule with intelligent anti-cancer and anti-cancer properties that "cats naturally bite mice without injuring poultry", and in particular, has various contributions in terms of medical beauty in the present invention.

The secondary components of the epidermal stem cells and multi-functional Chinese herbal medicine composition of the present invention are the main ingredients of Jiang Song Meiling Palace Secret Beauty and Beauty Mask, and the epidermal stem cells of the present invention for maintaining the skin and have a variety of functions Functional Chinese herbal medicine composition is the main ingredient combination, which also has a wider range of masks. It also has moisturizing, whitening, light spots, wrinkle removal, flat marks, anti-aging, and multi-functions that can increase the life of epidermal stem cells. A versatile mask. Among the main ingredients of Jiang Song Meiling's palace secret mask, the skin stem cells and multi-functional Chinese herbal medicine composition of the present invention are more refined. The effective ingredients are rose stem cells extracted from rose leaves and rose essential oil extracted from roses. , And Glycyrrhetin extracted from Glycyrrhiza uralensis, licorice flavonoids, and Rhodiola roseaside and Rhodiola rosea volatile oils for better.

The formula and auxiliary ingredients of the skin stem cells and Chinese herbal medicine composition with multiple functions of the present invention form a complex with soybean phospholipid and medicine, which can change the physical and chemical properties of the prototype drug, enhance the permeability of the skin and tissues, and thereby enhance the pharmacological effect. Improve bioavailability. Embedding hydroxypropyl-β-cyclodextrin and alginate (sodium alginate) can increase the main components of living human epidermal stem cells It is an inclusion compound and also a carrier, which enables the formula to achieve the effects of long residence time in the body and controlled release of epidermal stem cells that reach the "target". The synergistic effect of the transdermal absorption of the combination of azosterone and rhodiola rosea essential oil, which is recognized as the strongest penetration enhancer, is to assist the transdermal absorption of the main ingredients, and to achieve rapid maintenance and repair of skin quality. Stem cells and Chinese herbal medicines with various functions are excellent choices as penetration enhancers, stabilizers, thickeners, flavoring agents and the like.

100‧‧‧Mask

1‧‧‧ membrane body

11, 12, 13‧‧‧ opening

2‧‧‧ dressing layer

Steps S1 ~ S6‧‧‧‧

FIG. 1 is a schematic diagram showing a Chinese herbal medicine composition formed on a mask according to an embodiment of the present invention.

FIG. 2 is an exploded view showing a Chinese herbal medicine composition according to an embodiment of the present invention when it is included in a mask.

FIG. 3 is a flowchart showing a Chinese herbal medicine composition used for making a mask according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described with reference to FIGS. 1 to 3. This description is not intended to limit the embodiment of the present invention, but is an example of the embodiment of the present invention.

The Chinese herbal medicine composition of the present invention contains the following weight percentages of raw materials: grape skin or Polygonum cuspidatum root extract 5% to 11%, mulberry leaf extract 0.5% to 1.1%, scutellaria baicalensis extract 5% to 11%, and tripterygium root extract 0.7% ~ 1.3%, brown algae extract 3% ~ 9%, rose extract 1% ~ 5%, rhodiola extract 1% ~ 5%, licorice extract 0.5% ~ 6.5% and additives 51% ~ 83% .

Further, the grape skin or Polygonum cuspidatum extract includes no less than 770 mg / L of resveratrol, the mulberry leaf extract includes no less than 50 mg / L of ecdysterone, and the Scutellaria baicalensis extract includes no less than Baicalin at 770mg / L, the tripterygium root extract includes not less than 70mg / L asiaticoside, the brown algae extract The substance includes not less than 570 mg / L of alginate, the Rhodiola rosea extract includes not less than 70 mg / L of Rhodiola rosea essential oil and the salidroside of not less than 170 mg / L, and the licorice extract includes not less than Licorice flavone less than 170 mg / L and glycyrrhizin not less than 120 mg / L, and the rose extract includes rose leaf stem cells not less than 170 mg / L and rose essential oil not less than 70 mg / L.

Preferably, the grape skin or Polygonum cuspidatum extract includes 770-830 mg / L of resveratrol, the mulberry leaf extract includes 50-110 mg / L of ecdysterone, and the Scutellaria baicalensis extract includes 770-830 mg / L. L. Scutellariae, the tripterygium root extract includes 70 ~ 130mg / L asiaticoside, the brown algae extract includes 570 ~ 630mg / L alginate, and the rhodiola extract includes 70 ~ 130mg / L Rhodiola rosea oil and 170-230mg / L salidroside. The licorice extract includes 170-230mg / L of licorice flavone and 120-180mg / L of glycyrrhizin and the rose extract includes 170-230mg / L. Rose leaf stem cells and 70 ~ 130mg / L rose essential oil.

Specifically, the grape skin or Polygonum cuspidatum extract includes 800 mg / L of resveratrol, the mulberry leaf extract includes 80 mg / L of ecdysterone, and the scutellaria baicalensis extract includes 800 mg / L of baicalin The tripterygium root extract includes 100 mg / L Asiaticoside, the brown algae extract includes 600 mg / L alginate, and the Rhodiola rosea extract includes 100 mg / L Rhodiola volatile oil and 200 mg / L Rhodiola rosea The licorice extract includes 200 mg / L of licorice flavonoids and 150 mg / L of glycyrrhizin and the rose extract includes 200 mg / L of rose leaf stem cells and 100 mg / L of rose essential oil.

In the Chinese herbal medicine composition of the present invention, grape skin or Polygonum cuspidatum root extract, mulberry leaf extract, scutellaria baicalensis root extract, tripterygium root extract, and brown algae extract are used as the main components, and the grape skin or Polygonum cuspidatum extract contains Resveratrol has antibacterial, anti-oxidant, and cell repairing effects; ecdysterone contained in mulberry leaf extract has the effect of promoting collagen synthesis and cell growth; baicalin in scutellaria baicalensis extract has row excretion. The function of pus and repairing cells; the asiaticoside contained in tripterygium root extract has the effect of promoting cell regeneration, division and collagen synthesis; and the alginate contained in brown algae extract has strong absorption and maintains a moist environment Ability, and can improve the regeneration ability of epidermal cells and so on. In summary, The main component has the function of activating human epidermal stem cells and epidermal cells. As for the rose extract, Rhodiola rosea extract, and licorice extract as sub-components, the rose leaf stem cells contained in the rose extract have anti-allergic effects, prevent skin redness, wrinkle removal, and strengthen the skin barrier. Roses Flower essential oil can inhibit melanin and have good moisture absorption and moisturizing ability; Rhodiola rosea volatile oil contained in Rhodiola extract can promote the absorption of ingredients through the skin, while salidroside can prevent oxidation and reduce the body Inflammatory factors such as the pro-inflammatory cytokine TNF-α; and Glycyrrhiza uralensis contained in licorice extract can inhibit the formation of melanin and scavenge free radicals, while licorice flavonoids have antioxidant effects. The herbal composition of the present invention obtains the additive effects of moisturizing, whitening, light spots, wrinkles, flat marks, and anti-aging of the skin through the interaction between the main ingredients and the minor ingredients. In detail, the additives include humectants, thickeners, preservatives, solubilizers, lubricants, plasticizers, emulsifiers, surface coating agents, dispersants, flavoring agents, acidity regulators, and carriers. One or more of the group.

The humectant may be selected from one or more of the group consisting of sodium hyaluronate, hyaluronic acid, urea, lactic acid, lactate and glycerol. The carrier includes soy extract, potato extract, and azolone. It is worth mentioning that the weight percentage of the sodium hyaluronate is 2% to 8%; and the weight percentage of the soybean extract is 5% to 11%, and the weight percentage of the potato extract is 7% to 13%. And the weight percentage of the Azone is 1% to 5%, and the soybean extract includes not less than 770 mg / L of soybean lecithin, and the potato extract includes not less than 970 mg / L of hydroxypropyl-β -Cyclodextrin. For example, the soybean extract may include 800 mg / L of soybean lecithin, and the potato extract may include 1000 mg / L of hydroxypropyl-β-cyclodextrin.

The herbal composition of the present invention can be applied to be prepared into a lotion, an essence or a facial mask, etc., for more convenient and good external application to the skin. For example, a mask 100 shown in Figs. 1 and 2 includes a film-shaped body 1 and a dressing layer 2. The film-shaped body 1 has a plurality of openings 11, 12, 13 corresponding to the eyes, nose and mouth of a human face. . The dressing layer 2 is arranged on the surface of the film-shaped body 1, and the Chinese herbal medicine composition of the present invention is arranged on the dressing layer 2. Specifically, the film-like body 1 is a non-woven fabric. The total volume of the Chinese herbal medicine composition of the present invention contained in the dressing layer 2 is 1 liter, and the total weight of various raw materials is 1000 mg. Various raw materials and actual dosages are shown in Table 1 below:

The method of preparing the mask from the Chinese herbal medicine composition of the present invention will be described in detail with reference to FIG. 3 below: a potato extract containing hydroxypropyl-β-cyclodextrin and a brown algae extract containing alginate are mixed with water and passed through The magnetic stirrer completely dissolved the potato extract and brown algae extract in water to obtain a first mixed solution (step S1).

Grape skin or Polygonum cuspidatum root extract and soybean extract were dissolved in tetrahydrofuran at a weight ratio of 1: 1, and after magnetic stirring at 40 ° C for 1 hour, the tetrahydrofuran was recovered through reduced pressure, and chloroform was added. After dissolving, filtering, After evaporation, chloroform was finally removed and dried under vacuum to obtain a pale yellow sticky solid, namely a resveratrol phospholipid complex (step S2).

Blend resveratrol phospholipid complex with other main and auxiliary materials, including mulberry leaf extract, scutellaria baicalensis extract, tripterygium root extract, Rhodiola rosea extract, licorice extract and rose extract, and use an appropriate amount of absolute ethanol Dissolve, slowly add it dropwise to the first mixed solution under magnetic stirring, continue stirring for a certain time, leave it for a certain time, and then filter to obtain a second mixed solution (step S3).

Polyvinylpyrrolidone, sodium lauryl sulfate, and deionized water are thoroughly stirred at 90 ° C. to dissolve, and homogenized to prepare an aqueous phase (step S4).

Heat polyvinyl alcohol, cetyl wax or stearyl alcohol (carbon chain is 16 ~ 18), Azone, Rhodiola rosea oil, and Glycyrrhizin through a water bath to 85 ° C for thorough mixing, and homogenize to make an oil phase (step S5).

Finally, add the oil phase to the water phase, and then take glycerin, propylene glycol, hydrolyzed sodium hyaluronate, carson, hydroxyphenyl ethyl ester and stir in the same direction. Add the second mixed solution from step 3 at 75 ° C and continue to stir. Add rose essential oil at about ℃ to correct the taste and let it cool. Then adjust the ph value to 6.6 ~ 6.7 with triethanolamine solution, stir well to a slightly thick liquid, and then absorb it with a high-temperature sterilized non-woven fabric. After disinfection and sterilization, finally use an aluminum bag. The packaging is completed (step S6).

The following is a human-tested test of a mask containing the Chinese herbal medicine composition of the present invention. In this test, it was divided into experimental group and control group. The experimental group was a subject using a mask containing the herbal composition of the present invention, and the mask used in the control group had less ingredients than the main mask in the herbal composition of the present invention compared with the mask used in the experimental group. That is: grape skin or Polygonum cuspidatum extract containing resveratrol, mulberry leaf extract containing ecdysterone, baicalone extract containing baicalin, tripterygium root extract containing asiaticoside and alginic acid Salty brown algae extract. In addition, the experimental group and the control group further distinguished between those with skin defects and those with normal skin. The details are as follows: the total number of subjects: 64 people, 32 men and women each. Experimental group: (1) Those aged 30 to 65 years with skin defects. 8 males and 8 females; (2) those aged 26 to 65 years with normal skin. 8 males and 8 females. Control group: (1) those aged 30 to 65 years with skin defects. 8 males and 8 females; (2) 26-65 years old with normal skin, 8 males and 8 females. Those with skin defects include: darker skin on the face and front neck: 2 men each; male and female; severe wrinkles on the face and front neck: 2 men each; male and female; face and front neck Dry skin: 2 males and females each; oily skin on the face and frontal necks: 2 males and females on the skin; 2 spots on the facial and frontal neck skin: 2 males and females on the face; And those with obvious scars on the skin of the front neck: 2 for each of male and female; face And those with slack and inelastic skin on the front neck: two men and two women; and skin aging: two men and two women.

Those with normal skin include 14 men and women aged 26 to 45, 11 men and women aged 46 to 55, and 7 men and women aged 56 to 65.

Test area: The forehead, chin, left cheek, right cheek and corner of mouth, and the edge of the front neck are mainly used as test areas.

Prerequisites of the test: (1) 2 to 3 days before the test, the subject's face and front neck are prohibited from using various beauty care products, edible drugs and health care foods. (2) Before the test, the subject has uniformly cleaned the face and front neck skin, and wiped clean with a dry tissue. In addition, the subject was sitting in the room for at least 40 minutes with exposed skin on the face and front neck, and was asked to reduce friction and maintain ease on the face and front neck.

Test time: 1 evaluation per month, continuous testing for 90 days, 3 evaluations in total

Effectiveness scoring criteria: first, the formula ~ clinical symptom score improvement rate: (pre-experiment symptom score-post-experiment symptom score) / pre-experiment score X100% to calculate the improvement rate, and then use the following criteria to evaluate the effect .

For this test, all 64 people successfully completed the test throughout the whole process, and obtained 64 copies of the complete test results. The test results were divided into: significant effect: symptom score improvement rate ≧ 70%: effective: symptom score improvement rate ≧ 50%: Minimal effect: no significant improvement in symptoms or worsening of symptoms, improvement rate of symptom score is <50%.

Statistical processing: All data were statistically analyzed using SPSS13.0 statistical software. Measurement data were expressed as mean ± standard deviation (X ± S). The t test was used to compare the symptom scores before and after the experiment in the two groups of subjects, and the x2 test was used to compare the clinical effectiveness of the two groups of subjects.

Table 2 and Table 4 are the comparison of clinical symptom scores before and after the experiment in the experimental group and the control group, and Table 3 and Table 5 are the reference results of the comparison between the experimental group and the control group before and after the experiment. From Table 5, it can be obtained that the total effective rate of moisturizing of the mask containing the Chinese herbal medicine composition of the present invention is as high as 88%, the total effective rate of whitening is 88%, the total effective rate of mottled spots is 69%, the total effective rate of wrinkle removal is 69%, and the flat marks are always effective. The efficiency is 63% and the total effective rate of skin rejuvenation is 88%. In addition, no adverse reactions such as fever, flushing, edema, or pruritus were found in the skin of the experimental group.

Those in the experimental group aged 30 to 65 with skin defects (see Table 3) were significantly effective in moisturizing, whitening, skin rejuvenation, pale spots, wrinkles and flat marks. Those in the experimental group aged 26 to 65 years with normal skin (as shown in Table 5) were significantly effective in moisturizing, whitening, and rejuvenating the skin after use. In other aspects, they were slightly effective because they had no symptoms of the defect. .

Those in the control group aged 30 to 65 years with skin defects (see Table 3) were more effective in whitening, while those with younger skin, scarring, and other skin defects were only slightly effective. Those in the control group aged 26 to 65 years with normal skin (see Table 5) had significant effects in whitening after use, but were slightly effective in moisturizing and skin rejuvenation. Other areas were originally free of the defect. Symptoms are slightly effective.

In summary, under the same conditions, the conventional control group, the formulation composition and the manufacturing method of the present invention have been tested under the same conditions, and the formulation composition and the preparation method of the present invention are clearly shown. Manufacturing methods, using embedded drug materials and transdermal infiltration technology, and the most important cutting-edge leading applications of biomedical molecules that can proliferate, activate, repair, moisturize and regulate human epidermal stem cells, and can also prevent and prevent skin cancer, cancer stem cell growth The effective proportion of the composition of the medical molecule in an appropriate proportion has shown in the test results that it has a variety of effects on moisturizing, whitening, light spots, wrinkles, and flat marks, especially to improve skin rejuvenation, which proves that it is effective The invention of a dose composition and a method for manufacturing the same is effective for maintaining skin epidermal stem cells and its multiple functions. It is a novel and progressive invention that can be applied in the industry. It has already been granted in accordance with the requirements for granting invention patents. The reason for the patent right is to file a patent application in accordance with the law. It is still convenient for the examiner to examine and support the examiner in detail and grant the patent in this case at an early date.

However, the above description and description are only illustrative of the preferred embodiments of the present invention. Those with ordinary knowledge of this technology may make other modifications based on the scope of the patent application defined below and the above description, but these modifications are still It is the spirit of the present invention and is within the scope of the rights of the present invention.

Claims (10)

A Chinese herbal medicine composition for skin epidermal stem cells, including the following weight percentages of raw materials: grape skin or Polygonum cuspidatum root extract 5% ~ 11%; mulberry leaf extract 0.5% ~ 1.1%; scutellaria baicalensis extract 5% ~ 11%; Tripterygium root extract 0.7% ~ 1.3%; Brown algae extract 3% ~ 9%; Rose extract 1% ~ 5%; Rhodiola rosea extract 1% ~ 5%; Licorice extract 0.5% ~ 6.5% ; Potato extract 7% ~ 13%; and additives 51% ~ 83%, wherein the grape skin or Polygonum cuspidatum root extract includes no less than 770mg / L resveratrol, and the mulberry leaf extract includes no less than 50mg / L ecdysterone, the Scutellaria baicalensis extract includes not less than 770mg / L baicalin, the tripterygium root extract includes not less than 70mg / L asiaticoside, and the brown algae extract includes not less than 570mg / L alginate, the Rhodiola rosea extract includes not less than 70mg / L rhodiola oil and 170mg / L salidroside, the licorice extract includes not less than 170mg / L Licorice flavonoids and glycyrrhizin not less than 120mg / L, the rose extract includes rose leaf stem cells not less than 170mg / L and rose essential oil not less than 70mg / L, and the potato extract Comprising no less than 970mg / L hydroxypropyl -β- cyclodextrin. The Chinese herbal medicine composition according to claim 1, wherein the grape skin or Polygonum cuspidatum extract includes 770-830 mg / L of resveratrol, the mulberry leaf extract includes 50-110 mg / L of ecdysterone, and the yellow The tincture extract includes 770-830mg / L baicalin, the tripterygium root extract includes 70-130mg / L asiaticoside, the brown algae extract includes 570-630mg / L alginate, and the rhodiola extract The substances include 70 to 130 mg / L of Rhodiola rosea essential oil and 170 to 230 mg / L of salidroside. The licorice extract includes 170 to 230 mg / L of licorice flavonoids and 120 to 180 mg / L of licorice and the rose. The extracts include 170 ~ 230mg / L rose leaf stem cells and 70 ~ 130mg / L rose essential oil. The Chinese herbal medicine composition according to claim 1, wherein the grape skin or Polygonum cuspidatum extract includes 800 mg / L of resveratrol, the mulberry leaf extract includes 80 mg / L of ecdysterone, and the Scutellaria baicalensis extract includes 800mg / L baicalin, the tripterygium root extract includes 100mg / L asiaticoside, the brown algae extract includes 600mg / L alginate, and the rhodiola extract includes 100mg / L rhodiola oil And 200 mg / L salidroside, the licorice extract includes 200 mg / L of licorice flavone and 150 mg / L of glycyrrhizin and the rose extract includes 200 mg / L of rose leaf stem cells and 100 mg / L of rose essential oil . The Chinese herbal medicine composition according to claim 1, wherein the additives include wetting agents, thickeners, preservatives, solubilizers, lubricants, plasticizers, emulsifiers, surface coating agents, dispersants, flavoring agents, acidity One or more of the group consisting of a modulator and a carrier. The Chinese herbal medicine composition according to claim 4, wherein the wetting agent is one or more selected from the group consisting of sodium hyaluronate, hyaluronic acid, urea, lactic acid, lactate, and glycerin. The Chinese herbal medicine composition according to claim 5, wherein the weight percentage of the sodium hyaluronate is 2% to 8%. The Chinese herbal medicine composition according to claim 4, wherein the carrier comprises a soybean extract and an azatone. The Chinese herbal medicine composition according to claim 7, wherein the weight percentage of the soybean extract is 5% to 11% and the weight percentage of the azotonone is 1% to 5%. The Chinese herbal medicine composition according to claim 7, wherein the soybean extract comprises soybean lecithin of not less than 770 mg / L. A mask includes: a film-shaped body having a plurality of openings corresponding to the eyes, nose, and mouth of a human face; and a dressing layer provided on the surface of the film-shaped body, and the dressing layer is provided with the item 1 as requested The Chinese herbal medicine composition according to any one of 8 to 8.