CN108143844A

CN108143844A - For maintaining the epidermal stem cells of skin and there is the Chinese herbal medicine composition and its facial mask of multiple functions

Info

Publication number: CN108143844A
Application number: CN201611097059.2A
Authority: CN
Inventors: 翁明家; 翁宇青
Original assignee: WRITER OF RESVERATROL BIOTECH R&D CO Ltd
Current assignee: WRITER OF RESVERATROL BIOTECH R&D CO Ltd
Priority date: 2016-12-02
Filing date: 2016-12-02
Publication date: 2018-06-12

Abstract

The present invention relates to a kind of epidermal stem cells for maintaining skin and Chinese herbal medicine compositions and its facial mask with multiple functions, include Grape Skin or polygoni cuspidati,radix extract 5wt%~11wt%, mulberry leaf extract 0.5wt%~1.1wt%, Radix Astragali extract 5wt%~11wt%, Gotu Kola extract 0.7wt%~1.3wt%, brown alga extract 3wt%~9wt%, rose extract 1wt%~5wt%, rhodiola root extract 1wt%~5wt%, Radix Glycyrrhizae extract 0.5wt%~6.5wt% and additive 51wt%~83wt%, and the effect of the epidermal stem cells with maintenance skin.

Description

For maintaining the epidermal stem cells of skin and there is the Chinese herbal medicine composition of multiple functions And its facial mask

Technical field

The present invention relates to a kind of Chinese herbal medicine composition, more particularly to a kind of epidermal stem cells and tool for being used to maintain skin There are many Chinese herbal medicine compositions and its facial mask of function.

Background technology

The having dry skin, be obscure of human skin aging or generation, spot, wrinkle, coarse, scar, relaxation, it is weak, sagging, What hole was thick etc. the problems such as being all from various inherent causes, living environment, eating habit, drug, endocrine and behavior and time It influences and is formed, opinions vary by each scholar of a variety of causes.But, it is exactly source to make a thorough investigation, that is, epidermal stem cells with Years are in growth and aging, thus its function is being reduced in decline and quantity, and are practised by external factor living environment, diet Used, drug, behavior etc. are destroyed and are damaged, is injured.That is, epidermal stem cells are presented aging, decline less, fail, declining to undermine and decline wound etc. now As, and the defects of derived beautifying skin.Observe infancy, boyhood, the brephic, the adolescence, the mature stage, midlife, in it is old The application on human skin in term and senescence phase develops, and five causes and effects to decline, also referred to as irreversible " skin is presented in the operating of principal series epidermal stem cells Physiological clock " phenomenon.

People are for the defects of skin to be improved has or the defects of prevented beautifying skin and light aging, naturally always Change or benefit attain the beauty of skin, then using a variety of different face beautifying health keeping products, also reach the beauty of certain degree really Effect.But in the streets most face beautifying health keeping products are since molecular weight is more than a five kilodaltons (dalton about 1.67x 10^-27Kilogram) It skin permeation and can not can allow absorption of human body, generate effect, skin layer can only be rested on so that, just as waxing, surface is seen for it It is next smooth, but not change article essence, can only take stopgap measures the face beautifying health keeping product that cannot be effected a permanent cure at last.Also the face beautifying health keeping product having is adopted The small material of molecular weight can penetrate into skin and reach absorption of human body, if but be unable to hyperplasia activation human epidermal stem cell, Reng You U.S.-Chinas are not The regret of foot.Such phenomenon is presented in the facial mask of current market, but is liked by people family of liking to be beautiful, each family of Taiwan TV media Ardent report Taiwan facial mask accounts for world wide sales 17%, and annual turnover comes at 100,000,000 or more, and for China's Mainland tourist Platform rushes to purchase the first place of article ranking list.Obvious facial mask has become that modern female cosmetology is daily to prefer to use necessity, if but energy Male is allowed also to prefer to use as cosmetology necessity, will be the further quantum jump of facial mask purposes.

Inventor notes imperial palace facial mask secret recipe, therefore quotes as the secondary constituent of the present invention and share common people.Invention People is familiar with the utilization field of stem cell, and has been to win China State Intellectual Property Office ZL201210191413.3 and United States Patent (USP) US 9439874；And " food formula and its manufacturer for a variety of stem cells of nutrition maintenance mastery of TaiWan, China patent Method " patent of invention person, in view of in the streets facial mask just rams, but demand be all only limitted to the one such as moisturizing, whitening, light spot, smoothing wrinkle or second, Do not have equal demand have more than multi-functional complete facial mask, inventor with four big functions all can be complete demand and attached flat trace again And resisting age of skin function, and hyperplasia is particularly useful for its formula and manufacturing method, activation, repairing, is moistened, adjusting people's epidermis Raw doctor's molecule innovation facial mask face beautifying health keeping product of stem cell, can hyperplasia activation people's table to combine imperial palace facial mask secret recipe and performance modern times Characteristic of the brand-new facial mask for the present invention is made in skin stem cell life doctor field, will oblige more extensively in common people, not only woman from now on It will more prefer to use, the sudden emergence facial mask fine work of " man also likes oneself skin rejuvenation ".

Script facial mask, mainly by suction-operated, cover film is formed when facial mask is applied on skin to the cleaning of skin, It is peelled off by 20min or so, at this point, intrafollicular sebum and dirt peel off together with facial mask, makes pore unobstructed, be conducive to sebum Excretion and nutritional ingredient osmotic absorption, and reach single demand or two kinds of demands or fresh few moisturizing, whitening, Eradicates spots, remove Wrinkle, flat trace, resisting age of skin six thirdly demand, and a kind of leading a variety of demands that can have six functions with facial mask of the present invention； The leading formula and its manufacturing method that can be used for maintenance mastery human epidermal stem cell in outstanding tip, expectation can be a dark horse in face now Film market, contribution common people like to be beautiful, wish the U.S. face good merchantable brand of skin rejuvenation family.

Earthworm, which is cut, can also not be divided into two extremely partly, and gecko, newt docking can still regenerate tail, that is these animals There is born power of regeneration stem cell, and the organ display at present that the mankind can have power of regeneration only finds in liver, hair, refers to First, skin, wherein being all categorized in skin progenitor cell in addition to liver.Skin progenitor cell is stretched in the mountain of Kyoto Univ Japan in 2006 It more delivers world's the first and generates " the iPS cells of omnipotent cell " from adult skin using mouse, University of Wisconsin-Madison in 2007 is pungent big James's thomsons are learned, were more delivered from being stretched in mountain in two different units on the same day November 20：Skin Cell is become and is grown up The iPS universal stem cells of class, and these stem cell transformations is successfully made to become some of organ, it is planted through to Skin Cell Enter specific four genes, can induce Skin Cell transformation, become a kind of universal stem cell of similar " embryonic stem cell ", and later Nobel's invention award was obtained in pairs.It is the universal stem cell for being only second to embryonic stem cell to illustrate skin progenitor cell.2016 4 The moon, Japan Chemical research institute went out the artificial skin containing accessory organs such as hair follicle, sweat gland and sebaceous glands with iPS cell culture, with Toward artificial skin do not have more than the phenomenon that, successfully grow hair after being transplanted to experimental mouse, it is expected to 5~10 years planted agents It uses with the mankind, new breakthrough is presented always for treating diseases, the functions of skin progenitor cell such as burn, serious baldness.

Skin progenitor cell is broadly divided into " epidermal stem cells " and " hair follicle stem cells " two major class, and epidermal stem cells are skin group In knitting specially can stem cell, be a kind of adult stem histological cells, can Proliferation, Differentiation be various cell components in epidermis, keep skin Normal epidermal structure, epidermal stem cells are detached from skin epidermis basal layer, and another scientist is also under epidermis Hair follicle side protuberantia at find also with the presence of stem cell, be defined as hair follicle stem cells, and confirm that these stem cells can not only Hair follicle is generated, and epidermis, sebaceous glands can be developed into.It is " table to be studied in the skin progenitor cell of discovery more also more deep Skin stem cell ", it only accounts for the 1%~10% of epidermis bottom cell, but for safeguarding that skin metabolism and surface of a wound maintenance have Significance, research shows that：Epidermal stem cells are the key sources of skin generation, maintenance, remodeling, about caused by a variety of causes Skin injury, defect, defect reparation all rely on the differentiation of epidermal stem cells, proliferation, migration, fusion and reach again more mesh 's.

Body endepidermis stem cell many places are in quiescent condition, and only in the case of skin damage or in vitro culture, this is thin Born of the same parents' division, proliferation are just apparent to be accelerated, and under normal circumstances, each epidermal stem cells can be dry by " Asymmetric division " generation one Cell and a committed progenitor, that is, of short duration amplifying cells (TA), in epidermis, the major function of TA cells is to increase end point eventually Change the quantity of cell；Secondly, similar with hemopoietic system committed progenitor, of short duration amplifying cells (TA) differentiation potential is limited, research Display：In epidermis it can only directed differentiation be horn cell, hair and sebum line, both Asymmetric division is present in table In scytoblastema bottom, and spinous layer and corneocyte are formed with the terminal differentiation of epidermal stem cells.Stem cell and TA cells with And terminally differentiated cells are distributed in the different space structure of epidermis, are referred to as " epidermal proliferative unit (EPU) ", and become in gradient Change.Another kind is " cell subsets Asymmetric division ", also referred to as " symmetrical fissions ", i.e., often divide can once generate two stem cells or Two progenitor cells in this way, when epidermal tissue is by extrinsic damage, can increase stem cell or noble cells by symmetrical fissions Quantity, so as to safeguard the needs stablized and have preferably corresponding organism of epidermis.In short, division can generate two sons every time Cell, one of them characteristic with stem cell script, and another daughter cell is divided into transit amplifying cells (TA), this TA Postmitotic cells are formed after 3~5 divisions, postmitotic cells are constantly changed as on basal layer upwards again " terminally differentiated cells " are finally come off with the formation of " scurf " from skin surface.Namely ancients' legend people is clay sculpture, daily It washes one's face, still skin of having a bath has the skin reason of the dirt not washed clean forever！

The source of epidermal stem cells may have：(1) induced differentiation of embryonic stem cells generates；(2) differentiated cell reversal production The stem cell that raw (3) mesenchymal stem cell is regenerated as at epidermal stem cells (4) hair follicle is that warehouse (5) Hematopoietic Stem of epidermal stem cells is thin Born of the same parents or other tissue stem cells with blood circulation migration or passerby to skin histology, under the stimulation of certain factors, due to dry thin The plasticity of born of the same parents and to epidermal stem cells transdifferentiationof.There are three most significant features for epidermal stem cells：Slow periodically (end point eventually Change), self-renewal capacity and skin basement membrane is sticked.This causes epidermal stem cells growth cycle, grows incubation period, is right Number growth period and plateau ratio epidermal cell are long, and the self-renewal capacity of epidermal stem cells can not only keep cell infinitely to increase Ability is grown, and natural differentiation increases and maintain the quantity of living cells into epidermal cell.

The skin progenitor cell research and development that progressed greatly are become outside universal stem cell has the function of the mankind similar to embryonic stem cell, into For the research emphasis of medically organization engineering skin because understand daily how many injury live in hospital to burning, scalding, wound and For the patient that other wounds cannot heal, it is equally short that skin changes skin source, for the U.S., have every year 600000 people for a long time by Diabetic ulcer decocts, and 600,000 people will extract skin and go treatment cutaneum carcinoma, and also 10,000~10,000 5 thousand people will carry out dermatoplasty To treat serious burn and scald, how many operations, which are operated on, wishes that wound quickly heals, and how many bald heads thirst for hair restorations and how many White hair wishes to become dark hair againIt is opposite for most common beautifying skin, skin can aging, can follow the string and show wrinkle, It is no longer delicate smooth etc., because the colour of skin of skin, gloss, moisten, fine and smooth and elasticity oneself all like to be beautiful institute of family as modern civilization people Lie in.Skin is exposed to the influence caused by mechanical pressure, chemical stimulation, ultraviolet light irradiation and microbiological attack etc., penetrates The mitosis of skin progenitor cell, which is realized, constantly to be grown and updates.The differentiation of skin progenitor cell can also by its surrounding tissue and The influence of the extrinsic factors such as extracellular matrix, such as：Irritation growth factor, KGF (keratinocyte growth factor), EGF (tables Skin growth factor), TGF (transforming growth factor), PDGF (platelet growth factor) and inhibition semiochemicals (such as adrenal gland Element) etc., be involved in skin proliferation regulation and control, it is all related with repairing with the recovery of skin, Stanford good fortune universities of the U.S. in 2007 and It is dedicated to the Christian Dior research and development centre of skin ageing research, develops " epidermal stem cells protection science and technology ", experimental result confirms, epidermis The protective capability of stem cell promotes 104%, is effective against aging and the injury of irritation micro-pressure.The same year exists with the bold and unconstrained doctor of advance sheet member Famous world's periodical《Gene and development》The test report of " mouse skin of aging becomes rejuvenation again " is delivered, his team's profit With the mouse of two years old (50 years old natural span of life in service life of suitable people or more), this mouse skin is weathered, they block always After the NFkB genes of mouse, with the course for the treatment of of two weeks after the specific skin care products of dermal application, skin is made to stop aging and promotes new life, Skin after maintenance, the appearance of gene appearance slowly is identical with the skin of newborn mice, very magical.

Although skin progenitor cell is found very omnipotent, in science and technology transplanting now, there are still have what is used on Limitation and difficult, main is stem cell there are source is insufficient and being not easy of taking and culture technique does not attain ripe generalization, Picture embryonic stem cell is although omnipotent, but source is extremely difficult there is having, and only miscarries or has an abortion to take to obtain, Not so must be to sacrifice a problem of life goes to rescue another life, do not conform to many religions, ethics, morals, law, and skin Stem cell is typically the steamed sandwich skin to acquire children, other seek to the dermatoplasty or training with self skin or allosome It supports, it is necessary to be to sacrifice a surface skin to go to save another surface skin, seem some burned patients or the U.S. integer of doctor, if utilizing The dermatoplasty of allosome, the considerations of having anti-host rejection, regardless of self or allosome skin is still and digs the eastern wall to repair the western one, The skin of young delicacy, the epidermal stem cells for melting back young vigor are gained in terms of beauty, really a kind of difficult, money Heavy obstacle！Then finding alternative becomes the research emphasis of modern doctor's aesthetics.

The previous science and technology of raw physician is oriented to activating skin epidermal cell and is generally recognised as the best approach effected a permanent cure, and only all adopts Using human body or " cytohormone " of animality or chemistry as resisting skin defect derived from skin aging, and have more Piece related patents disclose the anti-aging application of Some Circulating Factors, such as：1987, Japan Patent JP62019511A2, compound one kind As the ingredient for preventing skin aging in epidermal growth factor (EGF) to cosmetics, 1999, United States Patent (USP) US56306848, Using a kind of organic derived ingredient (guanidine derivative), as the anti-aging cosmetic composition of modification wrinkle, together Year, Japan Patent JP2001097844A2, using a kind of anti-aging ingredients of the Thymusserphyllum as cosmetics.Always To TaiWan, China invention patent application case number in 2003：No. 092101859, (bone is similar to contain chemoattracting cytoking ingredient Person secreted by myelocyte) for anti-aging cosmetics, TaiWan, China invention patent application case number in 2006：No. 095149902, with Cross-linked amnion as raw doctor's material method, such as the sufferer epidermis that promotes to burn regeneration or the shield as anti-aging smoothing wrinkle Skin facial mask, TaiWan, China invention patent application case number in 2008：103105080, with interstital stem cell extract (chemotactic factor (CF)) Applied to the reparation of skin aging, only these do not disclose can hyperplasia activation human epidermal stem cell be again simultaneous can inhibit cutaneum carcinoma and cancer The characteristic and normal stem cell of disease stem cell, particularly cancer stem cell are same to have hyperplasia, differentiation characteristic, if not intelligent can hyperplasia Activating stem cells also wouldn't together be excited with cancer stem cell incubation period, and be brought about one's own destructionIt is therefore very important！

It was discovered by researchers that with certain herbaceous plant extracts, after acting on skin, by transdermal infiltration, can promote Into skin metabolism, have the function that beautification or repair skin, such as Chinese patent CN102772354A is disclosed in one kind The composition of medicine whitening speckle-eliminating facial mask, it is wherein interior to be prepared containing Astragalus Root P.E, for another example Chinese patent： CN104666178A discloses a kind of skin whitening, moisturizing composition and facial mask, it is wherein interior to take object, giant knotweed extract containing extract of glycyrrhiza Be prepared, only fail to disclose these extracts be can effectively hyperplasia activation human epidermal stem cell and what molecule of extraction can Hyperplasia activates human epidermal stem cell and can promote transdermal molecule and have combine plus multiply effect.

Chinese herbal medicine is with a long history, from ancient times many in fight or active ingredient for burn and scald, wound, thus researcher with Modern stem cell basic medical goes to test that object can have hyperplasia to activate human epidermal stem cell.It temporarily begins to take shape in effort for many years, But go to make a search for burn and scald, wound entirely.And it is of the invention for the purpose of curing U.S., application on human skin rejuvenation can be reversed in demand Or the Chinese herbal medicine life doctor molecule of delay skin aging can hyperplasia activation human epidermal stem cell；And skin can be improved and hinder sight defect, Therefore use the hired facial mask principal component in imperial palace as stepping-stone, and make something perfect even more perfect again with the modern times can hyperplasia activation human epidermal stem cell Cutaneum carcinoma can be prevented by having both can add the effect of multiplying with inhibiting raw doctor's molecule of cancer stem cell and can promote skin permeation molecule, and asking, Proof through following existing experiment display and embodiment, the present invention have had one in the skin maintenance field of " the U.S. facial mask of doctor " Kind of novelty, progressive, economy in industry using creation.

Researcher find to have certain herbaceous plant extracts can hyperplasia activation human epidermal stem cell or epidermal cell, Such as：5 grades of Pharmacy of Burn Center of The First Hospital of Jiangxi Medical College Liu De " Radix Astragali induction epidermal stem cells proliferation in 2004 Build organization engineering skin treatment defect of skin " research, conclusion explanation：Under the experiment condition, it is thin that Radix Astragali can induce epidermal stem Born of the same parents are proliferated, and epidermal stem cells may participate in the formation of surface of a wound hair follicles outgrowth structure, have structure organization engineering skin complete correction skin The application prospect of skin structure and function.Liaodong College agricultural in 2006 and environment branch Song Ning " grape skin extract resveratrols Function and application " research, illustrate that resveratrol can promote the secretion of collagen in skin care and beautification function, maintain The elasticity and thickness of skin promote the activity of SOD, remove human free radical, anti-oxidant, improvement people's body-internal-circulation, increase skin Nutrition reduces wrinkle, promotes epidermal cell differentiation, inhibits melanin and skin whitening.The first attached doctor of University Of Nanchang in 2009 Pharmacy of institute Hu Xiang etc. " influence of the Radix Astragali to application on human skin epidermal stem cells proliferation activity " is studied, conclusion explanation：Radix Astragali can be effective Enhance the expression of application on human skin epidermal stem cells telomerase reverse transcriptase and in-vitro multiplication activity (reversing epidermal stem cells aging). Orthopaedics China of Zhejiang Provincial People's Hospital in 2007 holds up etc. that " alginate dressing and mEGF use in conjunction promote refractory wounds epidermal stem thin Born of the same parents are proliferated " research, wherein showing：Alginates have extremely strong absorbability, can absorb the liquid for being equivalent to 20 times of own wt, energy It effectively controls sepage and extends usage time.The surface of a wound can be kept to moisten and do not stick the surface of a wound, to maintaining moist environment, raising epidermis thin The power of regeneration of born of the same parents accelerates that epidermal cell is mobile, wound healing is significant is played.Researcher also has found original Against a kind of " moulting hormone " shelling, decortication, it can stimulate dermal cell to generate new table for this insect, shell-fish or class animal Skin, and change in quality, such as butterfly, silkworm, cicada or snake are all representative.And in K.Nakanishi in 1966 et al. for the first time in Taiwan Find that plant also has this substance and why plant can have to turn green and sprout again or evergreen in civil antitumor herbal medicine " one hundred days are green " A kind of important element, more later mammal, the mankind it has also been found that its growth and development, husking reproduction play a role, especially exist Plant kingdom exists extensive, and can largely extract, and seems Major Foods of the mulberry leaf into silkworm, and the moulting hormone in mulberry leaf is known as β-slough off Skin hormone, also known as ecdysterone can promote human epidermal cell to break up in Michael D in 1974 et al. by experiment in vitro, More in attached Nanfang Hospital Lee state virtue of Guangzhou Nanfang Medical Univ in 2009 etc., " ecdysterone is external to human epidermal stem cell recently The influence of proliferation " is studied, conclusion explanation：Ecdysterone (EDS) can promote human epidermal stem cell (hESCs) under condition of in vitro culture Proliferation, this acts on a concentration of 80mg.L of EDS^-1When it is the most notable.One of its facilitation mechanism may be that it promotes people's epidermis By the G0 phases (cell is in hibernation, may be temporary or permanent stop growing) ,/G1 phases, (cell started to give birth to stem cell Long, cell size increases, while generates RNA and synthetic protein, and main purpose is ready for DNA replication dna), into S Phase (being the DNA synthesis phases, in order to make two daughter cells after division similar, it is necessary to which duplication doubles content), then by the S phases Into the G2 phases, (during for DNA replication dna to mitosis, the content of endonuclear DNA becomes 4n by the 2n of G1 phases at this time.G2 Phase, there is also limitation points, checked whether DNA completes to replicate, whether cell grows to suitable size, and whether environmental factor is conducive to Cell division etc.)/M the phases, (in the synthesis of the dormant protein of cell in M stages phase, the energy of all cells concentrated complexity And regular cell division is to obtain two similar daughter cells), start and reversed the activity of epidermal stem cells.2010 Year Shuguang Hospital of Shanghai enemy phenanthrene etc. " asiaticoside promote human epidermal stem cell replicate and splitting action " research, explanation：Asiaticoside With collagen update is promoted, accelerate the functions such as wound healing, have facilitation, energy to the division of the human epidermal cell of in vitro culture Shorten epidermal cell time of fusion, there is facilitation and protection efficiency to epidermal cell proliferation.Hundred happy company of Switzerland in 2010 A variety of comprehensive study tests are carried out, the results showed that rose leaf stem cell can not only promote vigor and the longevity of skin progenitor cell Life, while the barrier function of skin can be enhanced, pressure caused by protection skin confrontation climate change.Guangzhou Ling Yu Co., Ltds are also It is equally tested with rose leaf stem-cell research, as a result illustrated：Rose leaf stem cell can protect epidermal stem cells and safeguard skin screen Barrier, smoothing wrinkle and anti-skin wrinkle be red, allergy is tall and erect effective." resveratrol is to UVB for the polished grades of continent People's Armed Police medical college Wang Yi in 2011 Damage the Two-dimensional Gel Electrophoresis variance analysis of HaCaT cytoprotections " research, conclusion explanation：Resveratrol can raise cell The expression of GAP-associated protein GAP is transcribed, and then protects and cell transcription is promoted to be normally carried out, on the other hand, also by raising MAGOH eggs White activity promotes epidermal cell differentiation and plays protective action to ultraviolet B radiation (UVB).

Researcher find it is a kind of herbaceous plant it is natural resist element, antibiotic, enable plant high in face of sunshine According to, night in severe winter is cold, wind and weather, smog dew frost, the invasion that bird will peck, worm will gnaw, even the micro- life of root will be eaten into, and the life that can stand erect It deposits, that is, resveratrol life doctor's molecule that skin, leaf, the root of current 72 kinds of plants are found, and in Grape Skin, polygoni cuspidati,radix, flower It sprouts, mulberry leaves discovery content highest, the continuous development test through perennial physician has the effect of surprising different to come out repeatly, Such as：Massachusetts Institute Technology doctors Jia Lunti in 2004 and sinclair doctor are engaged in from other anti-aging sides after limit food therapy Method claims anti-aging food materials feeding saccharomycete, nematode, drosophila with a variety of, as a result can be prolonged with this raw doctor's molecule of resveratrol For its long life up to 75%, display resveratrol can increase its sir2 (longevity gene) activity.Italian neurology research in 2006 Sub- ˙ doctors Qie Laili Lishan Mountain moral sieve exist《Contemporary Biology》Magazine is delivered：Feeding resveratrol can make most short-lived vertebra Animal Africa tooth carp (fish) service life extends more than 50%.On November 1st, 2006 Harvard University with American National geriatrics Research institute cooperates, and claims the screening of Alfalfa Extract food materials that can activate the long-lived base in sirt~1 from over one hundred kind by sinclair doctor research team 18 kinds of food materials of cause, and arranged using resveratrol as the umber one, can extending the mouse service life 15% through mouse experiment, (suitable human longevity prolongs It is 10 years long), this achievement report sets the world on fire, and global 5000 or more media report the mystery of this then resveratrol. Thereafter continue within 2006 further to do monkey anti-aging experiment, because of 40 years old natural span of life in service life of monkey, the natural span of life in service life of people It is 120 years old, is to have no idea to conclude conclusion of several years of anti-aging extension service life in the so long natural span of life in service life a few years, still In 20 years old monkey of the pungent laboratory of university of University of Wisconsin-Madison, there are the monkey for eating resveratrol and the monkey for not eating white lamb's-quarters alcohol of the same age By 8 years；Two kinds of monkeys characterize from appearance, from physical condition energy, have and eat the monkey vigor Peng Bo of resveratrol, hold Light shines, and the monkey for not eating resveratrol is senile, it is clear that the monkey that resveratrol is eaten in control is young many！Another scientist Every comparative test has been done for resveratrol absorption of human body dosage form, has been preferred, therefore as beauty treatment U.S. so that Transdermal absorption is relatively oral Hold sticking more beneficial to the absorption of human body for skin care products.

Researcher find certain herbaceous plant extract can anti-skin natural aging, such as：Rose is romantic, aromatic because of it Fragrance becomes the odor type of modern woman's favorite of all times, and even more name noble woman advocates luxury goods, the whole world with what rose bathed There are nearly 3/4 perfume all ingredients containing Rosa Damascana, in the fragrance in west, rose is referred to as " queen " in spending, is most It is ancient and occupy one of natural perfume material of suitable status.It is beautiful in colour charming, and the sweet fragrance of fragrance becomes west generation Valentine's Day Table offers object, is also the favorite of many name woman of China and foreign countries' history.

The main chemical compositions of Rosa Damascana are citronellol (rhodinol), geraniol, benzyl carbinol, fanesol and its ester Class, linalool, rose oxide, eugenol, methyl eugenol.The difference in number and its chemical composition on these aromatic substance contents It is different, the deep perfume (or spice) by favorite strong, romantic, sweet, the mysterious rose of women worldwide is more produced, it is in Volatile Oil Composition of Rhodiola Using Rush infiltration with antioxidant activity effect under be smoothly absorbed by the body, into blood, body fluid, with the rapid migration of the circulatory system is in complete Body, and may be dissipated by the eccrine gland of the sweat gland and whole body of the wing of nose, armpit, omphalos, groin and surrounding genital etc. everywhere The rose fragrant fragrance of long romance is sent out, so as to which people be helped to cover body odour, moderns are relaxing！

The research such as Jinan Hygienical School, Shandong Prov. CN) Li Ming in 2008 " the pharmacognostical study pre-test of rose ", illustrates rhodamine toner Oil is the precious raw material of industry extracted from rose, is known as " liquid golden ", is 1.5 times of gold value, using rich in V_A、V_B、V_C、V_E、V_KAnd tannic acid is (wherein containing V_CAmount is higher, than also high 8 times or more of Chinese Chinese gooseberry, can be referred to as vitamin C King) etc. beneficiating ingredients Rosa Damascana, can prevent skin aging, it is flat help skin microgroove, adjust the balance of skin.

" resveratrol is to natural aging mice H by January, 2010 Liaoning University of TCM Wang Pei etc.₂O₂Content and ATP enzyme are lived The experimental study of power influence ", the experimental results showed that：Resveratrol can remove free radical, reduce hydrogen peroxide in skin histology (H₂O₂) content and improve the vigor of adenosine triphosphate atp enzyme, thus it is speculated that and demonstrate its effect in terms of delay skin aging. " the reality that resveratrol influences natural aging mice T-SOD vigor and MDA contents such as Liaoning University of TCM Wu Jing east in 2010 Test research ", conclusion explanation：It is living that resveratrol can improve total number born (T-SOD) in natural aging mice serum Property, reduce malonaldehyde (MDA) content in skin histology.Resveratrol is by scavenging activated oxygen, to mouse skin ageing process There are certain control and influence, its skin aging can be delayed, and in dose dependent.2010 Nian Hou Liaoning University of TCM The different research unit such as Wang Pei, Liaoning Academy of Health Sciences Zhang Xiaoli, Liaoning Province's anal intestine hospital dermatology department Yi, respectively with identical Resveratrol does natural aging mice skin experimental study, and comprehensive each conclusion confirms：Resveratrol can improve mouse gluathione Peptide (GSH) and resisting oxidation free radical content reduce hydrogen peroxide (H in skin histology₂O₂) content and improve ATP enzyme vigor, and The damage of the mitochondrial DNA caused by naturally aging of skin can be prevented, the generation of mt DNA deletion mutation rates is reduced, reaches and delay The effect of skin histology aging." resveratrol is to natural aging mice skin for Liaoning Province's anal intestine hospital dermatology department Zhang Yi in 2011 etc. The experimental study of skin mitochondrial DNA damage ", conclusion explanation：Skin mitochondrial DNA caused by resveratrol can prevent naturally-aged Damage." resveratrol is cloudy to mouse aging GSH and anti-super oxygen by April, 2011 Liaoning University of TCM Wu Jing east, Zhang Xiaoli etc. The influence of ion radical changes of contents " is studied, and is discussed that aging is physiological phenomenon normal and complicated in life process, is machine The combined process of internal various biochemical reactions, with advancing age, free radical generation increase, and remove the relevant enzyme of free radical Activity decline and body's immunity decline etc. many factors be body occur aging important mechanisms.According to 2000 The researchs such as Hung confirm that the antioxidation of resveratrol is better than vitamin E and vitamin C, and can remove free radical, especially Hydroxy radical makes DNA from damage, also glutathione can be made to be in also original state by inhibiting the formation of glutathione disulfide State, so as to inhibit the formation of free radical.Conclusion explanation：Resveratrol can improve GSH and superoxide anion resisitance free-radical contents, The oxidation resistance of body is improved, has the function that effective delaying skin tissue aging, and closed without apparent with the height of dosage System.The positive equality of Yichuan aesthetic medicine institute Wu in 2011 is with " rhodiola root studies acute senile model mouse skin ", conclusion Explanation：Radix Phodiolae Polyose can significantly improve mouse aging skin histology hydroxyproline content, so as to which skin collagen be promoted to close Into having enhances skin elasticity, the effect of delay skin aging.The white sun of Dalian Polytechnic University waits the " system of licoflavone within 2015 Standby and its resisting age of skin function " is studied, conclusion explanation：Skin Cell collagen caused by licoflavone significantly inhibits UVB The generation of catabolic enzyme (mmp~L1), licoflavone mass concentration reduce the generation of mmp~L1 in 1 and 10 μ g/mL respectively 23% and 56%, illustrate that it has the function of resisting age of skin.

Researcher find certain herbaceous plant extract can anti-skin photoage, such as：Continent Central South University is old within 2008 Bright " UVA causes human fibroblasts and the photoprotection of keratinocyte damage mechanisms and resveratrol " doctoral thesis Research, conclusion explanation：Resveratrol can repair long wave ultraviolet (UVA) to human skin fibroblasts and H₂C₂T cell proliferation Damage by lowering the expression of iNOS mRNA albumen, reduces the synthesis of NO to have the function that repair ultraviolet injury.2009 The old bright grade of Nian8Yue China's Mainlands Central South University " resveratrol to long-wave UV radiation after skin fibroblasts be proliferated And the influence of nitric oxide synthase type expression " research, conclusion explanation：Long wave ultraviolet (UVA) can inhibit in vitro culture The proliferation of dermal fibroblast, the expression of Inducible nitric oxide synthase (iNOS), and resveratrol to UVA radiation damage into Fibrocyte has protective effect.2009 Nian9Yue China's Mainlands dept. of dermatology of affiliated hospital of medical university Jia Lili etc. are in " resveratrol The protective action skin injury caused to simulation solar radiation " research, conclusion explanation：Resveratrol adds antioxidant that can effectively prevent Shield simulation acute light injury like caused by solar radiation." resveratrol is to skin photoage by University Of Nanchang condition Xiao Dong in 2009 etc. Protective effect " research, conclusion explanation：Resveratrol can improve photoaging skin polyphenoils enzymatic activity, change oxidation production The content of object reaches anti-skin photoage effect.University Of Suzhou's leaf in 2011 is chatted about etc., and " resveratrol irradiates Co 60 CO gamma-rays Nematode service life and the influence of reproduction " is studied, the results showed that：Resveratrol can improve the service life of exposure nematode, increase oviposition Survival rate, the nematode plastochondria caused by protecting its mitochondrial function, mechanism of action that may mitigate free radical with resveratrol damage It is related." the protections of Resveratrol on Human Keratinocytes After UVB Irradiation such as continent Central South University Tang Hua in 2011 Effect " research, conclusion explanation：The resveratrol of various concentration is proliferated without apparent shadow Normal Human Keratinocytes (HaCaT) Ring, resveratrol but in vitro has the HaCaT cells after irradiation a protective effect, mechanism may with improving oxidase active, It is related to remove free radical.The protective effect of high concentration resveratrol is better than low concentration, shows this protective effect of resveratrol There is a certain amount to imitate relationship." resveratrol causes people's cutin to be formed ultraviolet B radiation to Medical University Of Tianjin's Wang Jingjing in 2012 etc. The protective action of cellular damage " is studied, conclusion explanation：Resveratrol can significantly inhibit the HaCaT cells production of UVB radiation-induceds The variation of raw MMP-1, MMP-9 and TIMP-1mRNA content, can have skin photoage certain protective action.West in 2012 " resveratrol is to the shadow of the UVB human skin fibroblasts MMP-1 levels irradiated and Apoptosis by peace university of communications Zheng Wen etc. Ring " research, conclusion explanation：Resveratrol can inhibit the liter of the human skin fibroblasts MMP-1 levels of UVB radiation-induceds Height, and cell apoptosis caused by UVB irradiates can be reduced, resveratrol may irradiate skin by this mechanism to ultraviolet light to be played Protective effect." resveratrol applies outside to erythema caused by simulation solar radiation medical university of Mainland China Wu Yan in 2012 etc. Inhibiting effect " research, conclusion explanation：Resveratrol external application can effectively prevention and treatment simulation solar radiation induction skin it is red Spot, it is especially more preferable with prophylactic use effect.Taiwan Fu Ren universities in 2010 thank great writer " Enoxolone derivative is to skin physiology Application " research, conclusion explanation：Radix Glycyrrhizae can be used as a kind of compound (third type hydrone of AQP~3 for improving the performance of AQP~3 Channel is a kind of small molecule (~30KDa) and is embedded on human epidermal cell film " water channel albumen " that function can promote water Point and small molecule such as glycerine transport.Many researchs are pointed out, are improved AQP~3 and are showed, can increase hyperplasia and cell migration, Enhance skin barrier, and then reach and promote skin wound healing) applied to the skin of the hypoproteinosis of AQP~3 and other related diseases Disease, delay skin aging." resveratrol irradiates descendant's cutin shape to dept. of dermatology of the People's Hospital of Changsha in 2012 Tang Hua etc. to ultraviolet light The influence expressed into cell AQP3 (aquaporin) " research, conclusion explanation：Resveratrol may be by caused by inhibiting UVB AQP3mRNA declines and improves oxidase active, removes the function of free radical, so as to delay skin aging.Guangzhou in 2014 " resveratrol causes skin to preventing and treating skin diseases institute Liu Qing etc. as NrF2 (nuclear factor E2-related factor 2) activator defence ultraviolet light The research of oxidativestress damage ", conclusion explanation：It activates the mechanism of NrF2 to may be by reducing Keap1 expression to resveratrol, NrF2 is promoted to assemble in core so as to protect HaCaT cells from the caused oxidativestress damage of UVA irradiations.Tianjin in 2014 Molecular nuclear medicine key lab of city Wang Yan etc. " the radiation-induced NLRP3 expression mechanisms of mechanism of resveratrol inhibiting " is studied, conclusion explanation： Resveratrol can significantly inhibit the β and NLRP3 transcriptions of radiation-induced 1L~1 and expression, and may be by sirt1~ NLRP3 approach plays a role.No. 1 Hospital Affiliated to Chinese Medical Univ dept. of dermatology thunder in 2015 defends etc. that " resveratrol is to UVA Irradiate the photoprotection of human fibroblasts " research, conclusion explanation：0.01mmol/L resveratrols may by inhibit 1L~ The 1 β and NF~kB and its MMPs of mediation expression, so as to which UVA be inhibited to irradiate the light injury caused by human fibroblasts.2007~ 2010 by Taiwan Jia Nan pharmacology University of Science and Technology cosmetics research institute Zhang Yashu, Liu Rongxiu and Nanjing Medical University Shen Gan, Beijing traditional Chinese medical science Medicine university history such as flies at the different academic research units, influences research, comprehensive each knot for the aging of application on human skin light jointly with rhodiola root Demonstration is real：Application on human skin light aging blemiss caused by rhodiola root really irradiates UVB/UVA has protection and maintenance effect, acts on machine Being made as rhodiola root, there is improvement UV irradiations to cause the reduction of type 1procollagen albumen performances amount；And the albumen table of MMP~1 It now measures increased situation and activities of antioxidant enzymes in Human keratinocytes can be increased, lipid oxidation reaction is inhibited to reach clear Free Radical, hence it is evident that improve skin histology hydroxyproline content, and then the content of collagen in extracellular matrix is maintained to increase Strong skin elasticity.2010~2015 years total by the old eastern wave of Guangdong Pharmaceutical University, Lanzhou University Tian Zhuohua, Bian Fang, Zhao Min, the Lanzhou military region The different academic and clinical units such as hospital dermatology department Yang Gui orchids, jointly with Radix Glycyrrhizae, rhodiola root, astragalus needle to application on human skin light aging shadow Research is rung, comprehensive each conclusion confirms：Radix Glycyrrhizae, rhodiola root, Radix Astragali are really to the application on human skin light caused by ultraviolet B radiation (UVB) irradiation Aging and Apoptosis have inhibition protection and repair, and protection mechanism is Radix Glycyrrhizae, rhodiola root, Radix Astragali can induce skin group Middle caspase~8, HSP27, HSP70 expression and up-regulation IL~10, SOD, NO expression are knitted, and inhibits the generation of MDA, TNF~α, Enhance skin oxidation resistance, promote oxygen radical removing and skin collagen synthesis, it is related to reduce Skin Cell apoptosis.

Researcher has found that certain herbaceous plant extract can promote skin-whitening, the effect of Eradicates spots, such as：Taiwan is expanded within 2009 Light University of Science and Technology cosmetics scientific research institution Zhang Tingyi etc. " research of resveratrol whitening characteristic ", conclusion explanation：Resveratrol can be with Effectively inhibit the enzyme activity of gill fungus mushroom Tyrosinase, and by ferment dynamic analysis results presumption, resveratrol should be Tyrosine The noncompetitive inhibitor of enzyme.And after resveratrol is added with kojic acid, extracellular Tyrosinase activity is inhibited to have and is added Multiply effect.Conducive to the exploitation applied to skin-whitening skin care products." resveratrol is to junket ammonia by Beijing Forestry University Yang Yang in 2011 etc. The dynamics research of the sour external inhibitory activity of enzyme ", conclusion explanation：Resveratrol has suppression to tyrosinase monophenolase and diphenolase It makes and uses, it, which may not only be applied to pigment, increases the clinical treatment of dermatoses (chloasma, freckle etc.), and available for cosmetics The colour of skin is brightened, is also applicable as food additives, become prevention fruits and vegetables brown stain and treats the good preparation of various pigment diseases. First quick grade " Primary Study of resveratrol white-skinned face function " the conclusion explanation of Qinhua Univ., Beijing's Life Science College history in 2011：In vain Veratryl alcohol has certain inhibiting effect to the proliferation of B16 melanocytes and intracellular tyrosinase really, so as to reduce The effect of whitening is played in the synthesis of melanin.Resveratrol, which is added, for cosmetics provides experimental basis as whitening agent. " application studies of cosmetic material white-skinned face function Evaluation " such as Beijing University of Chinese Medicine Liu Rong in 2011, conclusion explanation：White lamb's-quarters Reed alcohol significantly inhibits melanin generation, shows that resveratrol not only can effectively inhibit tyrosine enzyme activity Property, there are the potentiality for inhibiting melanin generation, and with stronger transdermal and saturating cell membrane function, there is preferable whitening effect Fruit.Inspection and quarantine technique center Qin Yao of Guangdong in 2013 etc. " research of animal model evaluation cosmetic material white-skinned face function ", knot It expounds bright：Ursin/resveratrol, which is used in combination, can significantly improve whitening effect, and the melanin granule in tissue is made to generate and divide Cloth significantly reduces.The experimental study of " resveratrol is to Tyrosinase activity inhibition " of Guangdong medicine institute Luo Liqiong in 2014, Illustrate that resveratrol can reduce the synthesis of human skin melanin, so as to reach whitening, treatment chloasma, freckle effect, one Under fixed optimum condition, resveratrol has tyrosinase significant inhibiting effect, as effector, that is, resveratrol concentration increases Greatly, the vigor of tyrosinase is gradually reduced, and half inhibiting rate IC50 theoretically may indicate that resveratrol for 5.3mmol/Lo It applies in cosmetic additive agent, to increase the whitening effect of cosmetics.Fudan University in Shanghai Ma Jing involves platform within 2003~2009 years Wan Jianan pharmacology University of Science and Technology cosmetics scientific research institution Zhang Yashu；And the letter Yin Zhidengxue research units of China Medical University in Taiwan, point Research is not influenced for application on human skin whitening effect with rhodiola root, comprehensive conclusion confirms：Rhodiola root can inhibit Tyrosinase active And melanin content is reduced, mechanism may be by adjusting tyrosinase mRNA and melanocyte is closed in the performance of albumen Into inhibition, it was demonstrated that rhodiola root treatment skin pigmentation disorder is safety and validity, is screened suitable for skin-care product former Material." rose extracting object inhibits melanin generation and its Mechanism Discussion " of China Medical University's Wang Xiao cloud tints in Taiwan in 2014 Research, conclusion explanation：Rose extracting object has tyrosinase significant inhibiting effect, and inhibits melanocyte melanin Generation has whitening effect, is good cosmetic additive agent.Taiwan Jianan Pharmacology University of Science and Technology Chen Hui is pure within 2010~2013 years With Chen Shilong respectively with " Radix Glycyrrhizae is designed in the formula of skin-lightening cosmetic " and " research and development of licorice extract and grinding for its skin efficiency Study carefully ", conclusion illustrates respectively：Licorice extract really has excellent anti-oxidant and whitening active, and have excellent safety With stability, have improvement to ultraviolet light color spot, be the potentiality having as excellent skin skin care products functional raw material.2011 ~2015 years Nanjing Medical University's Yue Xue shapes；Cure special Ying Long in Jilin Province Baicheng；And Tianjin University Of Traditional Chinese Medicine Wang Yan；Hainan Province's skin The medical researches units such as sick hospital Li Wen are studied respectively with Radix Glycyrrhizae for application on human skin whitening effect, comprehensive conclusion explanation：In Radix Glycyrrhizae Glabridin be at present in the world generally acknowledge most effective natural whitening agent, can inhibit tyrosinase activity and press down Dopachrome change processed and dihydroxy indole carboxylic acid (DHICA) oxidase active, can be by reducing free calcium in melanocyte The concentration of ion is a kind of quick, efficiently, green whitening, the addition of Eradicates spots cosmetics so as to inhibit the migration of melanocyte Agent, whitening skin lightening, Eradicates spots effect, for have have the Scavenging ability similar to SOD (superoxide dismutase) and The similar Ability of antioxygen free radicals of vitamin E and can reduce melanocyte formed it is related.

Researcher has found that certain herbaceous plant extract can help skin injury/wound healing, such as：Jiangxi doctor in 2004 5 grades of Pharmacy of Burn Center of the first affiliated hospital of institute Liu De are with " Radix Astragali is to induction human epidermal stem cell proliferation construction tissue work Journey skin treating skin defect medical experiment " is studied, conclusion explanation：Radix Astragali, which has the growth of people's epidermis ex vivo, to be promoted to make With, it can induce epidermal stem cells and be proliferated, tool maintenance defect of skin structure and function application.Shanghai Ocean University in 2011 opens uncommon etc. " Chinese medicine inserts pinctada fucata the influence of core wound healing effect and pearl amount " is studied, conclusion explanation：Resveratrol or Yunnan Baiyao is best to the effect of wound single treatment.Can remarkably promote body basic fibroblast growth factor (bEGF) and The expression of vascular endothelial growth factor (VEGF) so as to accelerate angiogenic growth, is conducive to the healing of wound.Taiwan section in 2012 Skill university Gao Yici " bacteria cellulose for comparing bio-compatible is repaiied with I film combination resveratrol of collagen applied to wound Multiple benefit " Master's thesis is studied, conclusion explanation：Through experimental result, using the bacterium fibre procatarxis containing resveratrol be with Good bio-compatibility and regulation and control hair reaction, are capable of providing the reparation that the preferable environment of damaged skin one carries out skin. Taiwan Jianan Pharmacology University of Science and Technology cosmetics Technological research institute Cai Xing Tianjin in 2009 " it is big that rhodiola root extract liquor is applied to burn and scald The discussion of mouse zootype " is studied, conclusion explanation：Rhodioside may be via reduction lipid peroxidation and the internal total antioxygen of increase Change and reduce the inflammatory factor in skin (TNF~α IL~1 β) and the maintenance effect caused by reaching after burn and scald evil.2008~ Guangzhou Nanfang Medical Univ Zheng's high cloud, Zhou Yunfeng in 2010 etc. respectively with ecdysterone to rabbit experimental skin trauma, defect rush Healing and Mechanism Study, comprehensive conclusion explanation：Ecdysterone can really promote the healing of the experimental skin trauma of rabbit, mechanism It may be related with the height expression of induction EGF-R ELISA (EGFR) and bFGF.Taiwan Zhong Tai Universities of Science and Technology leaf people in 2010 With " how fiber optics combination centella is in application and assessment of skin tissue engineering for electro-spinning gelatin " study, conclusion explanation：Accumulated snow Careless glycosides is released slowly in injured skin histology, promotes proliferation of fibroblast in skin, is had and is promoted wound healing, shortens The effect of healing time.2007~2014 years Zhejiang the People's Hospital Hua Qing；And Beijing Jishuitan Hospital field Peng etc. with algin (again Name sodium alginate, alginates) on refractory skin wound influence medical research and Skin scar is cured for the plate area surface of a wound The clinical effectiveness research of conjunction, conclusion explanation：Algin can treat refractory skin wound, can not only increase alkalinity into fibre The expression of Porcine HGF (bFGF) and the proliferation of epidermal stem cells are tieed up, and bFGF and epidermal stem cells phase can be passed through Mutual effect is healed with further promoting surface of a wound fast and high quality." the alginates such as Canada the university of manitoba Wu Jun in 2015 Effect in mouse holostrome damage union of wounded skin is promoted " research, conclusion explanation：Alginates are used for skin wound, can promote It is adhered in vivo into mesenchymal stem cell (MSCs), grows, breaks up, migrating, and then promote skin histology reparation, can more added Fast wound healing.

Researcher has found that certain herbaceous plant extract can help random skin flap survival, such as：Xijing hospital Dong in 2009 Yuan Mei etc. is with " resveratrol influences experimental study to the random flap of transplanting ", conclusion explanation：Resveratrol can substantially reduce After random flap transplanting, malonaldehyde (MDA) content and inhibition activity of myeloperoxidase, demonstrate the anti-of resveratrol in tissue Oxidation, the effect of Green Tea Extract damage so as to mitigate capillary endothelial cell in skin flap tissues, mitigate ischemic~Reperfusion injury Endothelial cell and leucocyte stick after wound, the radical damage effectivelying prevent in Ischemia-Reperfusion Injury.Another resveratrol is also Platelet aggregation can effectively be inhibited, so as to mitigate the damage of flap microcirculation, can mitigate flap ischemia for resveratrol and fill again The mechanism of action of damage is noted, so resveratrol, which has, improves the potentiality that ischemic skin flap survives.

Researcher has found that certain herbaceous plant extract can help skin wrinkle/flat cicatrix of skin, such as：U.S. in 2007 Zhang Yuanhao R＆D teams of Stanford good fortune university of state find in experiment on mice, as long as blocking the term single gene of entitled " NFkB ", just The aged skin of mouse can be allowed to restore young after two weeks courses for the treatment of are received.Well-known aesthetic medicine authority Nicholas Pei in the whole world Doctor Li Kang refers to when Skin Cell is in NFkB advanced activations state and the transcription factor with other AP~1, will generate Wrinkle of skin.Many researchs have shown that resveratrol is exactly the natural jinxs of NFkB, and doctor Zhang Yuanhao tries to make some two years old mouse NFkB in the Skin Cell of (the suitable mankind half are at the age of one hundred years old) is restricted, smears specific creams in mouse skin, that is, begin to obtain Skin Cell Stop aging, after two weeks, the gene and tissue for receiving therapentic part skin with mouse become rejuvenation！December 1 in 2007 The China Times in day Taiwan, United Daily News especially reprint this achievement in research.Moreau in 2007 is to 33 37~64 years old women's Face and skin of neck are total to surrounding with the activator of Sirt~1 (the double property contracting amino acids of such as resveratrol, Kluyveromyces sp) 1 time/d, As a result prove that the activator of Sirt~1 can increase the expression of the Sirt in mankind's normal skin fibroblast and epidermal cell~1, And Skin Cell aging and DNA damage caused by ultraviolet B radiation (UVB) can be reduced.Research shows that first time using Sirt~ The skin agings problems such as after 1 activator, the aquation of skin is obviously improved, wrinkle of skin, pigmentation are obviously improved. " influence of the resveratrol to collage synthesis and related gene expression " such as the Shenzhen City Second People's Hospital of in September, 2005 Zhu Qian is ground Study carefully, conclusion explanation：Resveratrol has application on human skin cicatricial tissue fibroblast proliferation an inhibiting effect, and in dosage/time according to Lai Xing.Another resveratrol has obvious inhibiting effect to I type and III procollagen type mRNA, to the inhibiting effect of I procollagen type mRNA It is stronger than III procollagen type.(one of most important feature of hyperplastic scar is exactly the deposition of excessive collagen, application on human skin and scar Collagen is mainly made of I types and III type) many reports are proved the Collagen Proliferation of hyperplastic scar based on I type, i.e. Type I collagen Increase more apparent than III type.Therefore the inhibiting effect that synthesize to I, III procollagen type of resveratrol, molecular mechanism may be from turn The expression of the horizontal down-regulation type Ⅰ and Ⅲ collagen type mRNA of record, so as to inhibit fibroblast synthesis collagen and reduce the drop of clostridiopetidase A Solution.In March, 2006, Zhu Qian was equal to《Chinese Natural medicine magazine》Deliver that " resveratrol increases human skin fibroblasts and rabbit ear The influence of natural disposition scar " treatise, conclusion explanation：(1) it is that it inhibits scar to increase that resveratrol, which reduces fibroblast proliferation index, One of raw approach, and in dosage/time dependence (two) as resveratrol concentration increases, under rabbit ear scar proliferation index Drop, matches with the experiment in vitro of scar.In July, 2006 Shenzhen the second the People's Hospital Zhu Qian etc. " resveratrol to three-dimensional train The influence of foster application on human skin fibroblasts from hypertrophic scars shrinkage character " clinic treatise, it was demonstrated that resveratrol is to application on human skin cicatricial tissue Fibroblast proliferation and I, III procollagen type mRNA have obvious inhibiting effect, and can reduce fibroblast proliferation index and Rabbit ear scar proliferation index.Conclusion shows：Resveratrol can really promote the fibroblastic contraction of cicatrix of skin of dimensional culture. The second the People's Hospital of the Zhuhai City of Guangdong in 2009, which likes to account for, flourish waits that " resveratrol is to fibroblasts to secrete atrial natriuretic peptide and brain natriuretic peptide Influence " research, conclusion explanation：Experiment finds secretion atrial natriuretic peptide and brain natriuretic peptide, white black false hellebore during fibroblasts from hypertrophic scars in vitro culture Alcohol can significantly inhibit atrial natriuretic peptide and brain natriuretic peptide to secrete, and illustrate that resveratrol has certain suppression to fibroblast proliferation and plumpness It makes and uses, effect may be related with up-regulation NO-cGMP signal paths.

Researcher has found that certain herbaceous plant extract and chemical composition tool promote transdermal penetration/moistening effect, such as： Soft " natural anti-reflecting oxide resveratrol through the research of the skin administration Transmission system " conclusion in Taiwan Changgeng Univ. Huang Chinese catalpa in 2008 Illustrate that resveratrol is better than ionic condition in the skin permeation function of non-ionic state.Beijing University of Chinese Medicine Liu Rong in 2011 It is studied Deng " application of cosmetic material white-skinned face function Evaluation ", conclusion is explanation：Resveratrol, which has, turns strong transdermal and thoroughly thin Film function.The intelligent grade in Tianjin University Of Traditional Chinese Medicine field in 2011 " the transdermal test in vitro research of resveratrol phospholipid composite ", conclusion explanation： Resveratrol phospholipid composite improves the hydrophily and lipophilicity of resveratrol, and accumulation infiltration capacity dramatically increases, drug release behavior Meet level-one drug release model.It promotees infiltration mechanism may be related with influencing the mobility of keratoderma structure and lipid layer. Chinese invention patent the present inventor ZL2012 10191413.3 in 2012 and the U.S. " are maintained also by patent of invention for nutrition Accomplish the food formula and its manufacturing method of a variety of stem cells ", illustrate with sodium alginate (also known as alginates) and same hydroxypropyl~β ~cyclodextrin embedding resveratrol etc. works as carrier for inclusion compound, can make " to be formulated " to reach and " be marked with reaching in vivo " length stays the time " The effect of target " controlled release.Raw material supplier in 2016 all show hydroxypropyl beta cyclodextrin cosmetic material field be used as stabilizer, Emulsifier, deodorant etc. can reduce stimulation of the organic molecule to skin and mucosa tissue in cosmetics, enhance the stabilization of active ingredient Property, prevent nutrient ingredient volatilization, oxidation, and increase slightly solubility essence, fragrance it is water-soluble, keep fragrance sustained release and persistently. China of the People's Hospital of Zhejiang in 2007 holds up etc. that " alginate dressing is with freeze-drying M-EGF (mEGF) to refractory wounds epidermis Stem cells hyperplasia " is studied, conclusion explanation：Alginates have extremely strong absorbability, can absorb the liquid for being equivalent to 20 times of own wt Body is often employed embedding medicinal and enters in vivo release, if simultaneously can effectively control sepage with liquid and extend usage time, maintains wet Moisten (moisturizing) environment.The grain husks of Taiwan Jianan Pharmacology University of Science and Technology Wu Cheng in 2013 " roseleaf extract is anti-oxidant with whitening work( Effect " research, conclusion explanation：Rose ethereal oil (500 μ g/mL and 1000 μ g/mL) under the dosage of high concentration really can be found that have The significant activity for inhibiting B16 melanocytes generation melanin.The thick polysaccharides that roseleaf is extracted wettability power with It is similar to sodium hyaluronate in the assessment of moisture-retaining capacity, i.e., rose extraction rose polysaccharide have be developed into natural moisture preserving ingredient and The potentiality of cosmetic material." mulberry-leaf extract is to skin moisture-keeping for the richly endowed grade in the Yiwu Entry-Exit Inspection and Quarantine Bureau of Zhejiang in 2014 field The clinical research of performance and elasticity ", conclusion explanation：Mulberry-leaf extract has the function of apparent skin moisture-keeping, moistens, and promote skin Skin elasticity, promotion and crease-resistant effect including thick elastic, net elasticity and consolidation degree.Dafeng City of Jiangsu Province in 2014 is born Pharma Inc. Chen Juan buildings etc. " influence of the sachalin rhodiola rhizome volatile oil to scutellaria glycosides Transdermal Absorption " are studied, conclusion explanation：1% Sachalin rhodiola rhizome volatile oil be combined 3% azone to the promotion osmotic effect of scutellaria glycosides preferably (general single use rhodiola root 1%, Azone 5% is that transdermal effect is good), it was demonstrated that rhodiola root transdermal effect." the penetrating agents such as Tianjin University Of Traditional Chinese Medicine Wang Yan in 2015 The influence of in-vitro percutaneous skin osmotic effect to glabridin " is studied, conclusion explanation：The transdermal absorption factor of glabridin is relatively low, promotees Penetration enhancer can effectively pass it through skin, and different penetrating agents can promote the transdermal penetration effect of glabridin, and using azone as most It is good.

Invention content

The present inventor in view of it is above-mentioned note certain Chinese herbal medicines have can hyperplasia activation human epidermal stem cell drug study Report is only only all applied to the research of the wound healings such as burn and scald, wound, there have been no for the face of beautifying skin skin care products at present On film, and these Chinese herbal medicines are certain does not have transdermal characteristic, it is difficult to penetrate into carry out in vivo can hyperplasia activation human epidermal stem cell, it is and dry The characteristic " hyperplasia " of cell, " differentiation " and the characteristic of cancer stem cell are just the same, so exciting normal human epidermal stem cell Afterwards, it is also possible to excite the misgivings of incubation period skin cancer stem cell！There is practical difficult and missing to be improved！Therefore expecting can provide One breakthrough design is to promote practical function, through grinding think of, design group system with great concentration, comprehensive collection its be engaged in stem cell for many years and preserve bank Experience and obtained China and U.S. patent Nos：For the food formula and its manufacturing method of a variety of stem cells of nutrition maintenance mastery Special honours, the specialized technical knowledge of accumulation Related product design production and marketing are solved with practical experience through grinding to think to design, and develop this hair eventually The bright epidermal stem cells for being used to maintain skin and Chinese herbal medicine composition and its facial mask with multiple functions, to provide men and women all Really practical facial mask technological precedence highly sophisticated products person.

Therefore, the purpose of the present invention is to provide a kind of epidermal stem cells for being used to maintain skin and having multiple functions Chinese herbal medicine composition and its facial mask, to improve the various problems of skin.

Technological means provides a kind of Chinese herbal medicine composition used by the problem of the prior art of the invention for solution, comprising such as The raw material of lower weight percent：Grape Skin or polygoni cuspidati,radix extract 5%~11%；Mulberry leaf extract 0.5%~1.1%；Radix Astragali Extract 5%~11%；Gotu Kola extract 0.7%~1.3%；Brown alga extract 3%~9%；Rose extract 1%~ 5%；Rhodiola root extract 1%~5%；Radix Glycyrrhizae extract 0.5%~6.5%；And additive 51%~83%, wherein, the Portugal Grape skin or polygoni cuspidati,radix extract include the resveratrol not less than 770mg/L, which is included not less than 50mg/L's Ecdysterone, the Radix Astragali extract include the Astragaloside IV not less than 770mg/L, which includes being not less than The asiaticoside of 70mg/L, the brown alga extract include the alginates not less than 570mg/L, which includes being not less than The Volatile Oil Composition of Rhodiola Using of 70mg/L and the rhodioside not less than 170mg/L, the Radix Glycyrrhizae extract are included not less than 170mg/L's It licoflavone and is done carefully not less than the rose leaf that the glabridin of 120mg/L and the rose extract are included not less than 170mg/L Born of the same parents and the rose ethereal oil not less than 70mg/L.

A kind of Chinese herbal medicine composition is provided in one embodiment of this invention, and the Grape Skin or polygoni cuspidati,radix extract include The resveratrol of 770~830mg/L, the mulberry leaf extract include the ecdysterone of 50~110mg/L, which includes The Astragaloside IV of 770~830mg/L, the Gotu Kola extract include the asiaticoside of 70~130mg/L, which includes The alginates of 570~630mg/L, the rhodiola root extract include the Volatile Oil Composition of Rhodiola Using and 170~230mg/ of 70~130mg/L The rhodioside of L, the licoflavone of the Radix Glycyrrhizae extract including 170~230mg/L and the glabridin of 120~180mg/L and The rose extract includes the rose leaf stem cell of 170~230mg/L and the rose ethereal oil of 70~130mg/L.

A kind of Chinese herbal medicine composition is provided in one embodiment of this invention, and the Grape Skin or polygoni cuspidati,radix extract include The resveratrol of 800mg/L, the mulberry leaf extract include the ecdysterone of 80mg/L, which includes 800mg/L's Astragaloside IV, the Gotu Kola extract include the asiaticoside of 100mg/L, which includes the alginates of 600mg/L, should Rhodiola root extract includes the Volatile Oil Composition of Rhodiola Using of 100mg/L and the rhodioside of 200mg/L, the Radix Glycyrrhizae extract include The licoflavone of 200mg/L and the glabridin of 150mg/L and the rose extract include 200mg/L rose leaf stem cell and The rose ethereal oil of 100mg/L.

A kind of Chinese herbal medicine composition is provided in one embodiment of this invention, which includes wetting agent, thickener, prevents Rotten agent, solubilizer, lubricant, plasticiser, emulsifier, surface capping agents, dispersant, corrigent, acidity regulator and carrier institute One or more of group of composition.

A kind of Chinese herbal medicine composition is provided in one embodiment of this invention, the wetting agent be selected from sodium hyaluronate sodium, sodium hyaluronate, One or more of group that urea, lactic acid, lactate and glycerine form.

A kind of Chinese herbal medicine composition is provided in one embodiment of this invention, and the weight percent of the sodium hyaluronate sodium is 2% ~8%.

A kind of Chinese herbal medicine composition is provided in one embodiment of this invention, which includes soy extract, potato Extract and azone.

A kind of Chinese herbal medicine composition is provided in one embodiment of this invention, and the weight percent of the soy extract is 5%~11%, the weight percent of the potato extract be 7%~13% and the weight percent of the azone be 1%~ 5%.

A kind of Chinese herbal medicine composition is provided in one embodiment of this invention, which includes Include the hydroxypropyl not less than 970mg/L not less than the soybean lecithin of 770mg/L and 7%~13% potato extract Group-beta-cyclodextrin.

A kind of Chinese herbal medicine composition is provided in one embodiment of this invention, comprising：One membranaceous ontology, it is a plurality of right to have It should be in the opening of the eye of face, nose and mouth；And a dressing layer, the surface of the membranaceous ontology is set to, is laid in the dressing layer There is above-mentioned Chinese herbal medicine composition.

The epidermal stem cells for being used to maintain skin of the present invention and Chinese herbal medicine composition and its facial mask with multiple functions, It can have the following advantages：The main object of the present invention by compound symbiosis can hyperplasia activate human epidermal stem cell effectively point Son and the characteristic of effective dose cooperation, outstanding choosing can synergy add the ingredient for multiplying effect and can transdermal infiltration reach expectable hyperplasia The human epidermal stem cell simultaneous components matching advantage that can inhibit cancer stem cell and take into account again is activated, except various composition material ingredients have Previous test result as basis as scientific evidence, and then as this intention new face pack prescription combination and manufacturing method, The prior art and usage are different from, more effectively shortcut hyperplasia can activate human epidermal stem cell, it is short, more that long benefit is picked in performance Efficiency makes up prior art in the regret that target is used still to have blemish in an otherwise perfect thing！As most characteristic multi-functional and mastery " people can be maintained Epidermal stem cells ", but have both can inhibit skin cancer cell can hyperplasia activation repairing moisten adjust human epidermal stem cell facial mask.

The formula group of the epidermal stem cells for being used to maintain skin of the present invention and the Chinese herbal medicine composition with multiple functions Into its active ingredient of main molecules with the resveratrol of Grape Skin/polygoni cuspidati,radix extraction, with the ecdysterone that mulberry leaf extract, with Huang The Astragaloside IV of stilbene extraction, with the asiaticoside that Gotu Kola extracts, the alginates extracted with brown alga (also known as algin, alginic acid Sodium), wherein resveratrol in 2011 by Li Yong armies of Hebei Medical University etc. " resveratrol is to dermatophyte antibacterial activity " Experimental study illustrates that resveratrol is equal to microsporum lanosum, trichophyton rubrum, trichophyton gypseum, acrothesium floccosum There is stronger fungistatic effect.

Resveratrol, which was delivered in 2012 through total institute scholar Qiu China of Taibei veteran etc., can inhibit the news of cancer stem cell and bangs The dynamic whole world was taught by Chicago,U.S University of Illinois John Pezzuto international well-known earlier in 1997《Science》Magazine It is potent anticancer mutagens to deliver resveratrol, can effectively inhibit the cellular activity of each process of cancer, that is to say in tumour " starting ", " enhancement ", " expansion " three occurred is substantially carried out the stage, and resveratrol can play the role of inhibition.And illustrate The effect that tumour is inhibited to generate is shown in the animal experiment pattern of mouse skin cancer, the mouse of suffering from skin cancer is in the white lamb's-quarters of feeding Reed alcohol replenishers are after 18 weeks, with it the number of skin neoplasin fewer than the mouse for not eating resveratrol 98%, it can will be disliked Property cell becomes normal cell again.

Kundu etc. has found that resveratrol can inhibit the degradation of IkB in the research of mechanism of resveratrol inhibiting mouse skin cancer, The form that NFkB is combined with IkB is made to be retained in cytoplasm, and the intragentic transcription of core can not be acted on, and then induces tumour thin The apoptosis of born of the same parents.Kundu etc., which also found, inhibits the expression of COX-2 in mice skin tissue to block the activation of MAPKs and AP-1, can Can be one of important mechanisms of the anti-cutaneum carcinoma of resveratrol.Roy P etc. have found that resveratrol can adjust PI3K/ by experiment on mice AKT signal paths inhibit mouse skin cancer.

" the possibility signals of Resveratrol Inducing application on human skin cancer cell-apoptosis such as Beijing Capital University of Medical Sciences Zhao winter sesame in 2011 Access " is studied, conclusion explanation：Resveratrol may make JNK protein phosphorylations first, into nucleus, play and inhibit Bcl-2 eggs White expression, raises Bax and caspase-3 protein expressions, and then cause Apoptosis.

Burnt intelligent qin of the China's Mainland Capital University of Medical Sciences in 2009 etc. is " resveratrol grows application on human skin cancer cell line A431 Inhibition " experimental study, conclusion show：Resveratrol has certain inhibiting effect to the hyperplasia of skin cancer cell strain A431, and Adverse reaction is weak compared with chemotherapeutic drugs Cisplatin (DDP), and resveratrol simultaneously can induce A431 generation apoptosis, may be with inhibiting Bcl-2 tables It reaches, promote Caspase-3 expression related.

The researchs such as Hao are found：Resveratrol can inhibit the life of application on human skin squamous cell carcinoma A431 cell strain transplanted tumor in nude mice Long, one of mechanism and then may activate P53, induction tumour is thin with MAPK/ERK signal transduction pathway in activated tumor tissue Born of the same parents' apoptosis is related.

" resveratrol is to melanin and Lang Gehan after simulation solar radiation by Shenyang Chinese Medical Sciences University Jia Lili in 2010 etc. The influence of this cell " is studied, conclusion explanation：Resveratrol is likely to a kind of competitive inhibitor of protein substrate no matter Cis- or transconfiguration all has apparent tyrosine protein kinase inhibitory activity, so as to inhibit B16 cell, prevents skin Skin blackening.Langerhans cell is intraepidermal " antigen presenting cell ", plays key effect in cutaneous immunisation reaction, largely Research confirms that ultraviolet light irradiation can lead to table intradermal immunization presenting cell (Langerhans) reduction or failure, so as to cause immune suppression System or even skin carcinogenesis, and resveratrol is combined antioxidant or is used alone, one of both can effectively protect simulation Melanin granule caused by solar radiation increases and Langerhans cell quantity is reduced, and reaches whitening, prevention cutaneum carcinoma effect.

Xuzhou City of Jiangsu Province institute of traditional Chinese medicine Zhai Xiao Xiangs in 2011 etc. " resveratrol to people's epidermoid carcinoma Cell Telomerase Activity and The influence of its Proliferation and apoptosis " is studied, conclusion explanation：Resveratrol can inhibit the proliferation of people's epidermoid carcinoma cell A431, induction Its apoptosis, mechanism may be with inhibiting the transcription of telomerase hTERT mRNA gene proteins to reduce the expression of its albumen, so as to drop The activity of low telomerase is related.It (in most tumour cell abnormal expression of telomerase activity in nucleus cell, and is examined in normal human cell Telomerase activation is not detected, telomerase activating is one of key link that malignant tumour occurs).

" the Resveratrol Inducing application on human skin squamous cell carcinoma apoptosis such as Guangzhou Women and Children's Medical Center's sieve pendant friendship in 2011 Proteomics " research, conclusion explanation：11 differential protein molecules regulated and controled by resveratrol are identified in total, including BAG1, PDCD11, BCLAF1, HSPA9, YWHAZ etc. 5 has confirmed the protein molecule closely related with Apoptosis, is Everybody deeply understands that the molecular mechanism of jervic alcohol for antineoplastic medicine provides foundation and clue.

The reports such as Johnson, resveratrol can inhibit melanoma cells to rely on the transcription of STAT3 and NFkB, lower - 1 converting Enzyme of cellular type Fas associated death region protein like cells interleukin inhibits albumen (cFLIP) and the expression of Bc1-xL, finally MAPK and ATM-Chk2-P53 signal paths are activated, start apoptosis program.

Yangs experiment in vitro is found within 2005, and resveratrol can inhibit the synthesis of melanoma cells oxygen radical, and It can inhibit its proliferation.

Disease prevention and control center Zhao Jing of Nantong City in 2008 etc. is " resveratrol is to people A375 and mouse B16F10 Melanoma cells are proliferated and the influence of apoptosis " treatise, result of study, discovery resveratrol is to people A375 and mouse B16F10 cells There is significant inhibited proliferation, in dosage effect dependency relationships, conclusion shows：Resveratrol can inhibit pernicious in vitro The proliferation of melanoma (MM) cell.

China's Mainland in June, 2009 Jiangsu Province Funing Center for Disease Control Gao Guihua et al., in " resveratrol is to pernicious The external and In vivo study of Melanoma Growth inhibiting effect ", conclusion explanation：In vitro and in vivo experiment confirms that resveratrol is effective The growth for inhibiting malignant mela noma, and it was found that its antitumor mechanism may by inhibit the expression of p-Akt albumen come It realizes.

Traditional Chinese Medicine University Of Guangzhou's Liu's Jingjing in 2010 " resveratrol is to Murine Malignant melanoma suicide gene therapy Synergistic effect " is studied, conclusion explanation：Experiment in vitro finds that resveratrol has growth inhibition effect, and to B16 to B16 cells Cell strain tk/GCV systems (suicide gene) have synergistic effect, and synergistic effect is related with gap connection.In vivo studies is found Resveratrol equally has synergistic effect to the suicide gene therapy of Murine Malignant melanoma.

The research such as Ningxia University Li Yong in 2011 " inhibiting effect that resveratrol grows black cancer cell A375 ", knot It expounds bright：The inhibiting effect that resveratrol is proliferated black cancer cell A375 is apparent, and resveratrol to A375 cells in G1 The retardation of phase and S phases have certain concentration dependant and time-dependent effect, and inhibition growth is simultaneously apoptosis-induced, in experiment It observes that typical apoptotic cell morphological feature occur in A375 cells, it is bright to show that resveratrol has the growth of black cancer cell Aobvious inhibiting effect.

" resveratrol is to A375 human melanoma cell strain angiotensins by Wuhan City No.1 Hospital Zhou Feihong in 2011 etc. The influence of II and its expression of receptor " is studied, conclusion explanation：Resveratrol with to malignant mela noma local renin~vasotonia The regulation and control of prime system system.Its regulatory mechanism is not by its angiotensin-conerting enzyme inhibitor (ACEI) effect, but passes through inhibition ATIR's expresses to realize.

Central-South hospital of Wuhan University tight moonlight in 2013 etc. " resveratrol to people's Human melanoma cell line A375 proliferation and The influence of apoptosis " is studied, conclusion explanation：Reach certain density resveratrol has apparent suppression to Humanmachine tumour A375 cells Proliferation function processed, mainly by the way that A375 Apoptosis (early apoptosis) is induced to inhibit its proliferation.

Dept. of dermatology of the second affiliated hospital of Xi'an Communications University times in 2015 builds text etc., and " resveratrol is to the skin of hypoxia inducible Melanoma cells A375 is invaded and the influence of epithelial cell interstitial conversion process " research, conclusion explanation：Resveratrol can inhibit The melanoma cells A375 proliferation and transfer ability of hypoxia inducible, mechanism may be with inhibiting the α eggs of HIF~1 of hypoxia inducible Epithelial cell interstitial conversion (EMT) process that reconciled on (hypoxia inducible factor) in vain is related.Resveratrol presses down possibly as the α of HIF~1 Preparation becomes the drug candidate of melanoma prevention and treatment.

" inhibiting effect that resveratrol is proliferated melanoma cells " such as Shandong Academy of Medical Sciences Meng De duckweeds in 2012 Research, conclusion explanation：Resveratrol can inhibit the proliferation of WM239 cells, and cell cycle change, apoptotic cell is caused significantly to increase Add.

The double action of resveratrol really intelligent now anticancer, suppression cancer " cat nature can sting mouse and not hinder poultry " Raw doctor's molecule, particularly has the various contributions of the U.S. effect of doctor in the present invention.

The present invention for maintain skin epidermal stem cells and with multiple functions Chinese herbal medicine composition it is secondary into Point using empirical formula skin maintenance facial mask principal component, and with the present invention for maintain skin epidermal stem cells and have The Chinese herbal medicine composition of multiple functions be main component collocation, had both facial mask more extensively and meanwhile possess moisturizing, whitening, light spot, Smoothing wrinkle, flat trace, resisting age of skin, can hyperplasia activation epidermal stem cells it is multi-functional, be simultaneous to develop most functions at all times Standby facial mask.In empirical formula facial mask main force ingredient, epidermal stem cells for maintaining skin of the invention and with a variety of The Chinese herbal medicine composition of function more marrow with rose stem cell of the active ingredient for rose leaf extraction and rose extracting Rose ethereal oil and glabridin, the licoflavone of Radix Glycyrrhizae extraction, rhodioside, the rhodiola root of also rhodiola root extraction are waved Hair oil, to keep on improving.

The formula of the epidermal stem cells for being used to maintain skin of the present invention and the Chinese herbal medicine composition with multiple functions is auxiliary Expect that ingredient forms compound with soybean lecithin and drug, the infiltration of proto-drug physicochemical property, enhancing skin and tissue can be changed Property, so as to enhance pharmacological action, improve bioavailability.It is embedded with hydroxypropyl-β-cyclodextrin and alginates (sodium alginate) Can hyperplasia activation human epidermal stem cell main component for inclusion compound, also as carrier, formula is made to reach length in vivo and stays the time Effect with reaching " target " epidermal stem cells controlled release.To generally acknowledge that the most strong azone of penetrating agent and sachalin rhodiola rhizome volatile oil are combined Transdermal absorption synergistic effect, assist principal component Transdermal absorption, mastery skin quality can quickly be maintained by reaching, be the present invention be used for It maintains and penetrating agent is used as in the epidermal stem cells of skin and the Chinese herbal medicine composition with multiple functions, stabilizer, thickener, is rectified The excellent selection such as taste agent.

Description of the drawings

Fig. 1 is the schematic diagram shown when the Chinese herbal medicine composition of an embodiment according to the present invention is formed in facial mask.

Fig. 2 is explosive view when display Chinese herbal medicine composition according to an embodiment of the invention is contained in facial mask.

Fig. 3 is the flow chart for showing Chinese herbal medicine composition according to an embodiment of the invention to be made in during facial mask.

Reference numeral

100 facial masks

1 membranaceous ontology

11st, 12,13 opening

2 dressing layers

S1~S6 steps

Specific embodiment

Below according to Fig. 1 to Fig. 3, and illustrate embodiments of the present invention.The explanation is not the implementation for the limitation present invention Mode, and it is one kind of the embodiment of the present invention.

The Chinese herbal medicine composition of the present invention includes the raw material of following weight percent：Grape Skin or polygoni cuspidati,radix extract 5% ~11%, mulberry leaf extract 0.5%~1.1%, Radix Astragali extract 5%~11%, Gotu Kola extract 0.7%~1.3%, brown Algae extract 3%~9%, rose extract 1%~5%, rhodiola root extract 1%~5%, Radix Glycyrrhizae extract 0.5%~ 6.5% and additive 51%~83%.

Furthermore, the Grape Skin or polygoni cuspidati,radix extract include the resveratrol not less than 770mg/L, mulberry leaf extraction Object is taken to include the ecdysterone not less than 50mg/L, which includes the Astragaloside IV not less than 770mg/L, the Thunder God Root extract includes the asiaticoside not less than 70mg/L, which includes the alginates not less than 570mg/L, the red scape Its extract includes the Volatile Oil Composition of Rhodiola Using not less than 70mg/L and the rhodioside not less than 170mg/L, the Radix Glycyrrhizae extract Including being not less than the licoflavone of 170mg/L and including being not less than not less than the glabridin of 120mg/L and the rose extract The rose leaf stem cell of 170mg/L and the rose ethereal oil not less than 70mg/L.

Preferably, the Grape Skin or polygoni cuspidati,radix extract include the resveratrol of 770~830mg/L, the mulberry leaf extract Ecdysterone including 50~110mg/L, the Radix Astragali extract include the Astragaloside IV of 770~830mg/L, Gotu Kola extraction Object includes the asiaticoside of 70~130mg/L, which includes the alginates of 570~630mg/L, the rhodiola root extract The rhodioside of Volatile Oil Composition of Rhodiola Using and 170~230mg/L including 70~130mg/L, the Radix Glycyrrhizae extract include 170~ The licoflavone of 230mg/L and the glabridin of 120~180mg/L and the rose extract include the rose of 170~230mg/L Leaf stem cell and the rose ethereal oil of 70~130mg/L.

Specifically, the Grape Skin or polygoni cuspidati,radix extract include the resveratrol of 800mg/L, which includes The ecdysterone of 80mg/L, the Radix Astragali extract include the Astragaloside IV of 800mg/L, which includes 100mg/L Asiaticoside, which includes the alginates of 600mg/L, and the rhodiola root which includes 100mg/L is waved The rhodioside of hair oil and 200mg/L, the Radix Glycyrrhizae extract include the licoflavone of 200mg/L and the glabridin of 150mg/L And the rose extract includes the rose leaf stem cell of 200mg/L and the rose ethereal oil of 100mg/L.

In the Chinese herbal medicine composition of the present invention, Grape Skin or polygoni cuspidati,radix extract, mulberry leaf extract, Radix Astragali extract, As principal component, the wherein resveratrol contained by Grape Skin or polygoni cuspidati,radix extract has for Gotu Kola extract and brown alga extract The effect of antibacterial, anti-oxidant, repair cell；Ecdysterone contained by mulberry leaf extract, which has, promotes collagen synthesis and cell The effect of growth；Astragaloside IV contained by Radix Astragali extract has effects that apocenosis and repair cell；Contained by Gotu Kola extract Asiaticoside have effects that promote cytothesis, division and collagen synthesis；And the alginates contained by brown alga extract Ability with strong absorbability with maintenance moist environment, and power of regeneration of epidermal cell etc. can be improved.To sum up, should Principal component has effects that the epidermal stem cells and epidermal cell that activate the mankind.As for rose extract, rhodiola root extract and Then as time ingredient, the rose leaf stem cell wherein contained by rose extract has antiallergy, avoids skin general Radix Glycyrrhizae extract The effect of barrier of red, smoothing wrinkle and enhancing skin, and rose ethereal oil can inhibit melanin and with good moisture absorption and moisturizing Ability；Volatile Oil Composition of Rhodiola Using contained by rhodiola root extract can promote ingredient to be absorbed through skin well, and rhodioside Can be anti-oxidant and inflammatory factor in body be reduced, such as inspire inflammatory cell hormone TNF-α；And the light contained by Radix Glycyrrhizae extract Radix Glycyrrhizae surely inhibit melanin formation and remove free radical, and licoflavone have effects that it is oxidation resistant.The medium-height grass of the present invention Medicine constituent is obtained through the interaction between principal component and secondary ingredient to skin moisturizing, whitening, light spot, smoothing wrinkle, flat trace And the addition effect of resisting age of skin,

Specifically, which includes wetting agent, thickener, preservative, solubilizer, lubricant, plasticiser, emulsification One or more of group that agent, surface capping agents, dispersant, corrigent, acidity regulator and carrier are formed.

The wetting agent may be selected from one in the group of sodium hyaluronate sodium, sodium hyaluronate, urea, lactic acid, lactate and glycerine composition It is a or multiple.And the carrier includes soy extract, potato extract and azone.It is noted that the sodium hyaluronate sodium Weight percent is 2%~8%；And the weight percent of the soy extract is 5%~11%, the potato extract Weight percent is 7%~13% and the weight percent of the azone is 1%~5%, and the soy extract includes being not less than The soybean lecithin of 770mg/L and the potato extract include the hydroxypropyl-β-cyclodextrin not less than 970mg/L.For example, The soy extract may include that the soybean lecithin of 800mg/L and the potato extract may include the hydroxypropyl of 1000mg/L Group-beta-cyclodextrin.

The Chinese herbal medicine composition of the present invention, which can be applicable to, is prepared into lotion, Essence or facial mask etc., with more convenient It is and outer for skin well.Such as Fig. 1, the facial mask 100 shown in 2, comprising membranaceous 1 and one dressing layer 2 of ontology, this is membranaceous Ontology 1 has a plurality of openings 11,12,13 corresponding to the eye of face, nose and mouth.The dressing layer 2 is set to the membranaceous ontology 1 Surface, and in the dressing layer 2 be laid with the present invention Chinese herbal medicine composition.Specifically, which is nonwoven fabric. And the total volume of the Chinese herbal medicine composition of the present invention contained by the dressing layer 2 is 1 liter, the gross weight of various raw materials is 1000mg.Various raw materials and actual dose are as shown in table 1 below：

1 ﹞ of ﹝ tables

Collocation Fig. 3 Chinese herbal medicine compositions that the present invention will be described in detail are prepared into the mode of the facial mask below：

Potato extract containing hydroxypropyl-β-cyclodextrin and the brown alga extract containing alginates are mixed with water, And potato extract and brown alga extract is made to be dissolved completely in water and obtain the first mixed solution (step through magnetic agitation S1)。

By Grape Skin or polygoni cuspidati,radix extract with soy extract with 1：1 weight ratio is dissolved in tetrahydrofuran, and in Magnetic agitation, through tetrahydrofuran is recovered under reduced pressure, adds chloroform after 1 hour at 40 DEG C, through dissolving, filtering, evaporating, finally removes It removes chloroform and is dried in vacuo, and obtain faint yellow glutinous shape solid, is i.e. resveratrol phospholipid composite (step S2).

Resveratrol phospholipid composite is mixed into other main materials and auxiliary material, including mulberry leaf extract, Radix Astragali extract, Gotu Kola extract, rhodiola root extract, Radix Glycyrrhizae extract and rose extract, and dissolved with appropriate absolute ethyl alcohol, in magnetic force Slowly it is added drop-wise in first mixed solution under stirring, continues stirring after a certain period of time, stands certain time, then mistake Filter is to get the second mixed solution (step S3).

It by polyvinylpyrrolidone, lauryl sodium sulfate and deionized water, is sufficiently stirred and dissolves at 90 DEG C, and Water phase (step S4) is made in matter.

Polyvinyl alcohol, spermaceti or stearyl alcohol (carbochain is 16~18), azone, Volatile Oil Composition of Rhodiola Using, glabridin are penetrated Heating water bath, to be sufficiently mixed, and homogenizes and oil phase (step S5) is made to 85 DEG C.

Finally, oil phase is added in water phase, then takes glycerine, propylene glycol, hydrolysis sodium hyaluronate sodium, Kathon CG, ethyl hydroxy benzoate by same One direction stirs evenly, and second mixed solution that step 3 is added at 75 DEG C continues to stir, and treats 50 DEG C or so addition rose anthosin Oily flavoring is let cool, then adjusts ph values to 6.6~6.7 with triethanolamine solution, is stirred to micro- magma shape, then with high temperature The nonwoven fabric disinfected absorbs, and sterilized, sterilizing finally completes (step S6) with aluminium bag packaging.

It is experiment of the facial mask through human test of the Chinese herbal medicine composition containing the present invention below.In the experiment, it is divided into Experimental group and control group.Experimental group uses the facial mask of the Chinese herbal medicine composition containing the present invention for subject, and control group is made Under facial mask is compared with facial mask used in experimental group, ingredient has lacked the principal component in the Chinese herbal medicine composition of the present invention, That is,：Grape Skin containing resveratrol or polygoni cuspidati,radix extract, contain Astragaloside IV at the mulberry leaf extract containing ecdysterone Radix Astragali extract, the Gotu Kola extract containing asiaticoside and the brown alga extract containing alginates.In addition, experimental group and right According in group and further discriminating between skin with handicapped and skin category normal condition person.It is as follows in detail：

The number of total subject：64 people, each 32 people of men and women's property.Experimental group：(1) 30~65 years old age and skin is defective Person.8 people of male, 8 people of women；(2) 26~65 years old age and skin normal condition person.8 people of male, 8 people of women.Control group：(1) 30~65 years old age and the defective person of skin.8 people of male, 8 people of women；(2) 26~65 years old age skin normal condition person, male 8 people, 8 people of women.The defective person of skin includes：Face and the more black person of forward neck portion skin：Male and each 2 people of women；Face and preceding Skin of neck has serious wrinkle person：Male and each 2 people of women；Face and foreneck subordinate's dry skin：Male and women each 2 People；Face and foreneck subordinate Oily person：Male and each 2 people of women；Face and the more apparent spottiness person of forward neck portion skin：Man Property and each 2 people of women；Face and forward neck portion skin significantly have scar person：Male and each 2 people of women；Face and forward neck portion skin are shown It relaxes and inelastic person：Male and each 2 people of women；And skin aging person：Male and each 2 people of women.

And skin category normal condition person includes：26~45 years old men and women totally 14 people, 46~55 years old men and women totally 11 people and 56~65 Year men and women totally 7 people.

Test position：Mainly using forehead, chin, left cheek, right cheek and the corners of the mouth and forward neck portion side as test area.

Test premise：(1) 2~3 days before test, the face of subject and forward neck portion are forbidden to use various cosmetologies Product, edible medicine and health-care food.(2) before test, subject has unified to clean face and forward neck portion skin, and with dry Facial tissue towel wiped clean.In addition, subject sits quietly at least 40 minutes in the room, face and forward neck portion skin are in exposed, and Given requirement to reduce the friction of face and forward neck portion and keep light.

Testing time：Monthly criticism 1 time, follow-on test 90 days, altogether criticism 3 times

Effect standards of grading：

First by formula~clinical symptoms integration score value improvement rate：(symptom integral point after symptom integral score value-experiment before experiment Value)/experiment before score value X100% calculate improvement rate, then using following standard rating effects.

Test is completed for all 64 people of this test are smoothly whole, complete 64 parts of test result meter is obtained, tests As a result it is divided into：

It is effective：Symptom integral score value Gai Shan Shuai≤70%：

Effectively：Symptom integral score value Gai Shan Shuai≤50%：

Minor effect：Symptom is not affected significantly or counter sees deterioration, symptom integral score value improvement rate ＜ 50%.

Statistical procedures：Total data is for statistical analysis using SPSS13.0 statistics softwares, measurement data mean ± standard deviation (X ± S) represents.Symptom score is more examined using t before and after two groups of subjects, and two groups of subjects are clinical Effect compares to be examined using x2.

Table 2 and table 4 are compared for experimental group and control group experiment using front and rear symptom scores, and table 3 and table 5 are experimental group Effect compares reference before and after being tested with control group.The moisturizing of the facial mask of the Chinese herbal medicine composition containing the present invention can be obtained from table 5 Total effective rate, which is up to 88%, whitening total effective rate 88%, Eradicates spots total effective rate 69%, smoothing wrinkle total effective rate 69%, flat trace, always to be had Efficiency 63% and skin rejuvenation total effective rate 88%.In addition, being observed through experimental group, have no and find that the skin of subject has hair The adverse reactions such as heat, flush, oedema or itch.

2 ﹞ of ﹝ tables

Symptom scores table before and after the defective person's experiment of 30~65 years old age skin in experimental group and control group：

3 ﹞ of ﹝ tables

30~65 years old age and the defective person's effect comparison sheet of skin in experimental group and control group：

4 ﹞ of ﹝ tables

Symptom scores table before and after 26~65 years old age and skin normal condition person test in experimental group and control group：

5 ﹞ of ﹝ tables

26~65 years old age and skin normal condition person's effect comparison sheet in experimental group and control group：

After 30~65 years old in experimental group and the defective person of skin (such as table 3) use, moisturizing, whitening, skin rejuvenation, Light spot, smoothing wrinkle and flat trace are all significantly effective.26~65 years old age in experimental group and skin normal condition person (such as table 5) are in making With rear, moisturizing, whitening and to skin rejuvenation all significantly effectively, the then summary when script is i.e. without the defect symptom of other aspects It is effective.

It is more effective in terms of whitening after 30~65 years old age in control group and the defective person of skin (such as table 3) use, And skin rejuvenation, go scar and other skin defects person only slightly effectively.26~65 years old age in control group and skin is normal After state person (such as table 5) use, in terms of whitening there is significantly effect, however then slightly have in moisturizing and skin rejuvenation Effect, other aspects are then because script is then slightly effective i.e. without the defect symptom.

In conclusion in the excellent control group of both own tradition and inventive formulation composition and its manufacturing method, same Test effect, obvious inventive formulation constituent and its manufacturing method are implemented under the conditions of one respectively, is using embedding medicinal former Material and transdermal infiltration technology and most important tip leading application can hyperplasia, activation, repairing, moisten, adjust human epidermal stem cell Raw doctor's molecule and taking into account can inhibit anti-cutaneum carcinoma processed, cancer stem cell life doctor's molecule effective dose proper proportion composition, Result of the test showed synergy its moisturizing, whitening, light spot, smoothing wrinkle, flat trace multi-effect, especially show skin rejuvenation As improving, it was demonstrated that used the present invention of effective dose composition and its manufacturing method effective thin for maintaining the epidermal stem of skin Born of the same parents and its multiple functions very haves novelty progressive and an invention for being applied in industry for one, and reality has met patent of invention The reasons why important document is awarded with patent right is given, whence proposes patent application, still prays that your juror can give examination and support in detail in accordance with the law, and Quasi- this case patent is grant early, and true feeling is when it's convenient.

Only above narration and explanation are only the explanation of presently preferred embodiments of the present invention, for ordinary skill Personnel work as can make other modifications, therefore these modifications should be according to claim defined above and above-mentioned explanation For spirit of the invention in the interest field of the present invention.

Claims

1. a kind of Chinese herbal medicine composition for the epidermal stem cells for being used to maintain skin, which is characterized in that include following weight percent The raw material of ratio：

Grape Skin or polygoni cuspidati,radix extract 5%~11%；

Mulberry leaf extract 0.5%~1.1%；

Radix Astragali extract 5%~11%；

Gotu Kola extract 0.7%~1.3%；

Brown alga extract 3%~9%；

Rose extract 1%~5%；

Rhodiola root extract 1%~5%；

Radix Glycyrrhizae extract 0.5%~6.5%；And

Additive 51%~83%,

Wherein,

The Grape Skin or polygoni cuspidati,radix extract include the resveratrol not less than 770mg/L,

The mulberry leaf extract includes the ecdysterone not less than 50mg/L,

The Radix Astragali extract includes the Astragaloside IV not less than 770mg/L,

The Gotu Kola extract includes the asiaticoside not less than 70mg/L,

The brown alga extract includes the alginates not less than 570mg/L,

The rhodiola root extract includes the Volatile Oil Composition of Rhodiola Using not less than 70mg/L and the rhodiola root not less than 170mg/L Glycosides,

The Radix Glycyrrhizae extract include the licoflavone not less than 170mg/L and glabridin not less than 120mg/L and

The rose extract includes the rose leaf stem cell not less than 170mg/L and the rose anthosin not less than 70mg/L Oil.

2. Chinese herbal medicine composition as described in claim 1, which is characterized in that the Grape Skin or polygoni cuspidati,radix extract includes The resveratrol of 770~830mg/L, the mulberry leaf extract include the ecdysterone of 50~110mg/L, Radix Astragali extraction The Astragaloside IV that object is taken to include 770~830mg/L, the Gotu Kola extract includes the asiaticoside of 70~130mg/L, described Brown alga extract include the alginates of 570~630mg/L, the rhodiola root extract includes the red scape of 70~130mg/L The rhodioside of its volatile oil and 170~230mg/L, the licoflavone of the Radix Glycyrrhizae extract including 170~230mg/L and The glabridin of 120~180mg/L and the rose extract rose leaf stem cell and 70 including 170~230mg/L~ The rose ethereal oil of 130mg/L.

3. Chinese herbal medicine composition as described in claim 1, which is characterized in that the Grape Skin or polygoni cuspidati,radix extract includes The resveratrol of 800mg/L, the mulberry leaf extract include the ecdysterone of 80mg/L, and the Radix Astragali extract includes The Astragaloside IV of 800mg/L, the Gotu Kola extract include the asiaticoside of 100mg/L, and the brown alga extract includes The alginates of 600mg/L, the rhodiola root extract include the Volatile Oil Composition of Rhodiola Using of 100mg/L and the rhodiola root of 200mg/L Glycosides, the Radix Glycyrrhizae extract include the licoflavone of 200mg/L and glabridin and the rose extract of 150mg/L The rose ethereal oil of rose leaf stem cell and 100mg/L including 200mg/L.

4. Chinese herbal medicine composition as described in claim 1, which is characterized in that the additive include wetting agent, thickener, Preservative, solubilizer, lubricant, plasticiser, emulsifier, surface capping agents, dispersant, corrigent, acidity regulator and carrier One or more of group formed.

5. Chinese herbal medicine composition as claimed in claim 4, which is characterized in that the wetting agent is selected from sodium hyaluronate sodium, glass is urinated One or more of group that acid, urea, lactic acid, lactate and glycerine form.

6. Chinese herbal medicine composition as claimed in claim 5, which is characterized in that the weight percent of the sodium hyaluronate sodium is 2%~8%.

7. Chinese herbal medicine composition as claimed in claim 4, which is characterized in that the carrier includes soy extract, Ma Ling Potato extract and azone.

8. Chinese herbal medicine composition as claimed in claim 7, which is characterized in that the weight percent of the soy extract is 5%~11%, the weight percent of the potato extract is 7%~13% and the weight percent of the azone is 1%~5%.

9. Chinese herbal medicine composition as claimed in claim 7, which is characterized in that the soy extract includes being not less than The soybean lecithin of 770mg/L and the potato extract include the hydroxypropyl-β-cyclodextrin not less than 970mg/L.

10. a kind of facial mask, which is characterized in that include：

One membranaceous ontology has a plurality of openings corresponding to the eye of face, nose and mouth；And

One dressing layer is set to the surface of the membranaceous ontology, is laid in the dressing layer just like in claim 1 to 8 Any one of them Chinese herbal medicine composition.