KR101505576B1 - Composition against striae distensae with the extract of asparagus officinalis - Google Patents

Abstract

The present invention relates to a composition for preventing or alleviating striae distensa containing an extract of Asparagus officinalis as an active ingredient. The composition is effective in promoting synthesis of collagen, inhibiting activity of elastase, moisturizing skin, and preventing or alleviating striae distensa.

Description

TECHNICAL FIELD The present invention relates to a composition for preventing or improving an expanding lineage containing asparagus extract,

The invention asparagus (Asparagus The present invention relates to a composition for preventing or ameliorating an enlarged scarlet fever.

The skin surrounding the human body is very elastic, but when the subcutaneous body tissue, muscle or fat, suddenly expands quantitatively, the skin can tear and fail to follow the rate of expansion. The skin such as thighs or abdomen can not follow the swelling rate of the subcutaneous body tissue, and the symptoms of thinly split or torn are often referred to as 'stretch marks'. The difference between wounds and stretch marks is that collagen and elastin can not keep up with the growth rate of body tissue, and wounds are caused by tissue destruction of the epidermis and collagen etc.

Stretch marks are also commonly referred to as stretch marks and are sometimes referred to in medical terms as 'striae distensae' or 'dilated ancestors'. Stretch is an atrophic linear banding lesion that appears on the skin of the area damaged by the pulling force. On the histological side, the epidermis is atrophied and the dermis is rearranged parallel to the skin by the collagen fibers being thin. The early lesions of the stretch appear to have a red line or band on the skin. If you look closely, it looks a bit darker than your normal skin, and when you touch it, it looks rugged. These lesions are called purple ancestors (striae rubra). The purple ancestors gradually turn white as time goes by, becoming less distinct, wrinkled and shrunken skin. This is called the white ancestor (striae alba). These white line marks appear different from normal surrounding skin and color, so they show a bad appearance of skin cosmetics.

The stretch marks are mainly on the arms, thighs, abdomen, buttocks, groin grooves, but also on the face, and stretch marks due to Cushing's syndrome may occur on the skin of the folded area. Also, stretch marks due to pregnancy are common in the abdomen and breasts. The stretch marks of obese patients tend to be less pronounced, less stomach, and more narrow than the stretch marks of Cushing's syndrome patients. In large-sized stretch marks, ulcers sometimes occur or skin cracks occur.

 On the other hand, stretch marks are caused by physiological or pathological factors that increase the adrenocortical hormone in the body, resulting in destruction of collagen and elastin fibers in the dermis layer due to their sensitivity and collagen growth rate have.

The types of stretch marks include the effects of adrenocortical hormones secreted to protect the fetus during pregnancy, and the pregnancy-induced stretch, adrenal cortex, which occurs when the skin enlarges due to the growth of the fetus or suddenly decreases after the birth. There are stretch marks caused by excessive stretching of the hormone, stretch due to obesity and diet, endocrine disease, chronic wasting disease, long-term use of steroids, excessive muscle exercise or stretching. It is more appropriate to say "improvement effect" than to treat because medicine is almost impossible to recover a round.

Stretch marks are likely to be cured early in development, but it is difficult to expect a satisfactory therapeutic effect after it has turned white. Conventionally, as a method of treating stretch marks (inflated ankles) at an early stage, there have been a method of treating using a Versa pulse laser or a method of applying retinoid acid to a site where an inflated ancestor has been developed. In addition, as a method of treating late-stage expansion arteriosus, retinoic acid was applied for several months while thinning the epidermis using a carbon dioxide gas laser (Ultrapulse CO2 laser) or an Erbium-YAG laser, There was a method of stimulating collagen remodeling by stimulating fibroblasts.

However, such a conventional treatment method of swollen filament treatment requires a treatment time of 6 months to 1 year, and it is difficult to show an improvement rate of 50% or more even after treatment, and in some cases, it may cause side effects such as erythema and pigmentation.

On the other hand, asparagus ( Asparagus officinalis ) is a monocot plant of the lily of the neck, which is also called the extinct or tropical. Its height is about 1.5 m, and its roots are long like rope and short cylindrical with pointed ends. Asparagine, known for its amino acids, is the name given to it because it was first discovered in this plant. It is a name given to asparagus, but when used as a vegetable, it refers to some kind of horticultural herbs. Among them, A. falcatus It also refers to only. Vine and long stretches of flu parameter's asparagus (A. plumosus) and things that Gary's friendly Asparagus (A. sprengeri) sagging downward, etc. are sometimes called ornamental asparagus, but collectively.

Whether such asparagus has been shown to prevent or ameliorate stretch marks is not known at all.

Korean Patent No. 10-0496524 entitled "Hanging Spray Composition" is a composition for a hangover decomposing composition comprising at least two kinds of extracts selected from the group consisting of asparagus extract, green tea extract, onion extract and plum extract . Korean Patent No. 10-0914381 entitled " composition for treating an enlarged filamentous follicle ") contains glucose and a physiologically applicable solvent and has an osmolality of 120% to 350% % Composition for treating an inflated filamentous fungus, which is capable of promoting the regeneration of skin by promoting collagen production in injured dermis tissues and thereby treating the dilated filamentous fungus.

The present invention provides a composition for external application for skin, which has an effect on prevention or amelioration of scarlet fever using natural extracts.

In order to achieve the above object, the present invention provides a cosmetic composition for preventing or improving stretch marks, which comprises asparagus ( Asparagus officinalis ) extract as an active ingredient in a first form.

The cosmetic composition may be, for example, any one selected from a lotion, a gel, a water-soluble liquid, a cream, an essence, a makeup base of an underwater type or a water-in-oil type, a foundation, a skin cover, a face powder, and a two-way cake.

On the other hand, in the cosmetic composition of the present invention, the asparagus extract may be obtained by extracting from a seed, an outpouring or a pure part of asparagus or a mixture thereof. For example, a powder of about 2 to 20 times, preferably about 2 to 5 times, water or a polar (C1-C4) alcohol containing methanol or ethanol, or a mixed solvent thereof is added to the ground powder of asparagus .

In the cosmetic composition of the present invention, the asparagus extract may be prepared by (a) adding asparagus a water or a lower alcohol (C1-C4) alcohol containing methanol or ethanol or a mixed solvent thereof to the asparagus at a temperature of 10 to 30 ° C ; (b) filtering the resulting extract, followed by concentration and lyophilization; And (c) extracting the concentrate or lyophilized product obtained after the step (b) by adding at least one solvent selected from purified water, ethanol, butylene glycol, and propylene glycol, and extracting .

Meanwhile, in the cosmetic composition of the present invention, the extraction in step (a) may be any one extraction method selected from, for example, cold extraction, reflux cooling extraction, hot water extraction, ultrasonic extraction and ultra high pressure extraction.

On the other hand, in the cosmetic composition of the present invention, the asparagus extract is preferably added in an amount of 0.0001 to 90.0% by weight based on the dry weight of the whole composition.

On the other hand, the second aspect of the present invention provides an external preparation for skin preventing or improving an enlarged filament characterized by containing an asparagus extract as an active ingredient.

The composition for external application for skin may be used as a skin irritant such as PLASTERS, LOTIONS, LINIMENTS, LIQUIDS AND SOLUTIONS, AEROSOLS, EXTRACTS, OINTMENTS, And may be any one selected from FLUIDEXTRACTS, EMULSIONS, SUSPENSIONS, CAPSULES, CREAMS, soft or hard gelatin capsules, patches, and sustained release agents.

On the other hand, in the composition for external application for skin of the present invention, the asparagus extract may be obtained by extracting from a seed, an outpour, or a pure part of asparagus or a mixture thereof. For example, a powder of about 2 to 20 times, preferably about 2 to 5 times, water or a polar (C1-C4) alcohol containing methanol or ethanol, or a mixed solvent thereof is added to the ground powder of asparagus .

In the composition for external application for skin according to the present invention, the asparagus extract may be prepared by (a) adding asparagus a water or a lower alcohol (C1-C4) alcohol containing methanol or ethanol or a mixed solvent thereof, Extracting from the temperature; (b) filtering the resulting extract, followed by concentration and lyophilization; And (c) extracting the concentrate or lyophilized product obtained after the step (b) by adding at least one solvent selected from purified water, ethanol, butylene glycol, and propylene glycol, and extracting .

Meanwhile, in the composition for external application for skin of the present invention, the extraction in step (a) may be any one extraction method selected from, for example, cold extraction, reflux cooling extraction, hot water extraction, ultrasonic extraction and ultra high pressure extraction.

On the other hand, in the composition for external application for skin of the present invention, the asparagus extract is preferably added in an amount of 0.0001 to 90.0% by weight based on the dry weight of the whole composition.

Meanwhile, the composition for external application for skin of the present invention includes, for example, a pharmacologically acceptable carrier and excipient including lactose, glucose, sucrose, mannitol, potato starch, corn starch, calcium carbonate, calcium phosphate and cellulose; Binders comprising starch, tragacanth gum, gelatin, molasses, polyvinyl alcohol, polyvinyl ether, polyvinylpyrrolidone, hydroxypropylcellulose, methylcellulose, ethylcellulose and carboxymethylcellulose; A pulverizing agent comprising agar, starch, gelatin powder, carboxymethyl cellulose sodium, carboxymethyl cellulose calcium, crystalline cellulose, calcium carbonate, sodium hydrogen carbonate and sodium alginate; Lubricants including magnesium stearate, talc and hydrogenated vegetable oils; And coloring agents. Adjuvants such as stabilizers, dissolution aids and transdermal absorption accelerators may be further added.

On the other hand, the standard dose of the composition for external application for skin of the present invention may be varied depending on the individual characteristics of the patient, and a practitioner skilled in the art will be able to determine the ideal dosage amount for the external preparation for skin of the present invention The dosage plan can be set. Reference can also be made to determine appropriate dosages, and may be generally determined in vitro or in animal models.

Means that the asparagus extract is added to the composition to such an extent as to exhibit the effect of preventing or improving stretch marks (expansion filament) from the cosmetic composition and the composition for external application for skin of the present invention , And in the case of a composition for external application for skin, various components may be formulated into various forms by adding various components for drug delivery and stabilization.

Asparagus of the present invention officinalis extract as an active ingredient exhibits an excellent effect in promotion of collagen synthesis, inhibition of elastase activity, skin moisturization, prevention or improvement of swollen filaments.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

[Example 1: Preparation of asparagus extract]

The asparagus larvae were washed and diluted three times with an aqueous solution containing 70% (v / v) ethanol for 5 hours, refluxed, and filtered through Whatman # 5 filter paper. The filtered extract was concentrated and lyophilized at 50 DEG C or lower, and then an aqueous solution of 40% (v / v) 1,3-butyleneglycol was added so as to contain 2.0% by weight of the obtained lyophilized product, To obtain an asparagus extract.

[ Experimental Example  1: Measurement of promoting effect of type 1 procollagen biosynthesis]

In order to confirm the effect of accelerating the biosynthesis of type 1 procollagen, which is a skin substrate component of the asparagus extract obtained in Example 1, TGF-beta was used as a positive control, and a procollagen assay We tried to carry out as follows.

Human dermal fibroblasts isolated from epidermal tissue of newborn infants were purchased from Modern Tissue Technology (MTT, Korea) and cultured in DMEM / F-12 (3: 1) medium supplemented with 10% fetal bovine serum FBS) was added and cultured at a concentration of 1 × 10 ⁴ cells / cm ² . When 70 ~ 80% of the cells were grown, the cells were subcultured at a ratio of 1: 3, and the cells cultured in the third to fourth passages were used for the experiment.

To measure the amount of procollagen, fibroblasts were cultured in a 48-well plate at a concentration of 90% or more. Then, the asparagus extract was added at a concentration of 0.02% by weight, 0.01% by weight and 0.005% by weight, After that, the amount of procollagen liberated in the medium was measured using a procollagen type-1 C-peptide EIA kit (MK101, Takara, Japan) Respectively.

Name of sample content Promoting effect of procollagen biosynthesis (%) Asparagus extract 0.02 wt% 89.8% 0.01 wt% 75.2% 0.005 wt% 69.2% TGF-beta 200 nM 84.7%

As a result, in the treatment of 0.02% by weight of asparagus extract, the biosynthesis of procollagen was promoted by 89.8%, which was similar to that of TGF-β, a cell signaling substance (Table 1).

From the above results, it was confirmed that the extract of asparagus promotes excellent procoagulant biosynthesis.

[ Experimental Example  2: Elastase  Activity inhibition effect measurement]

In order to measure the promoting effect of elastin production of the asparagus extract obtained in Example 1, the following experiment was conducted by treating the extract of asparagus with human fibroblasts directly collected from humans.

Human fibroblasts were placed in a culture flask and cultured until they were about 70-80% grown. After the asparagus extract was treated for 1 day, the cell culture was collected and the degree of elastin production was measured using a commercially available elastin measuring device (Bieth J: Biochem med., 11, 350-357 (1974), Schwartz DE : J. Invest Dermatol., 86, 63-68 (1986)). That is, the activity of elastase was measured using the absorbance of a color change caused by the decomposition of NA of Suc- (Ala) 3 NA which is a substrate of elastase. As a control group, asparagus extract-treated group was used. The measurement results are shown in Table 2 below.

Name of sample content Elastase activity inhibition rate (%) Asparagus extract 0.02 wt% 79.8% 0.01 wt% 71.2% 0.005 wt% 64.2%

As a result, the asparagus extract showed an inhibition rate of 79.8% at a concentration of 0.02 wt%, and it was confirmed that the inhibitory rate of the elastase activity was increased in a concentration-dependent manner (Table 2). From the above results, it was confirmed that the excellent elasticity-enhancing effect of the present invention.

[Experimental Example 3: Measurement of water hygroscopicity]

In this experimental example, moisture absorption of the asparagus extract was measured. The asparagus extract and distilled water obtained in Example 1 were respectively saturated and absorbed into dried epidermal cells of a person directly taken from a human, the amount of absorbed water was measured, and the mass change was measured after drying for 48 hours.

The amount of water absorbed per skin cell unit mass was compared from each measured mass change. This experiment is a method for comparing the amount of water absorbed to the epidermal cells, and is used as one method of predicting the moisturizing effect even in the human skin. Specifically, human epidermal cells were dried to make the amount of water contained per unit mass constant, and then the weight of each epidermal cell sample and the amount of water contained were measured using the Kaiser method. The epidermal cells in which the water content was measured were carried on the asparagus extract and the purified water for 24 hours each time to saturate and absorb the epidermal cells. The epidermal cells in which the water was saturated and absorbed were taken out from the cells, and the weight was weighed and a part thereof was collected and contained in the Kaiser Moisture Analyzer The amount of water per unit mass was measured. Thereafter, it was dried again under reduced pressure for 48 hours and weighed. After the weight measurement, a part of the sample was taken and the water content was measured by the Kaiser method, and the amount of water per unit mass contained therein was measured according to the following equation (1).

Moisture content Initial dry state Saturation  After re-drying Asparagus extract 400 890 640 Purified water 400 700 400

As a result, it was confirmed that the epidermal cells saturated with the asparagus extract contained much more water in the re-drying process than the epidermal cells saturated and absorbed by the purified water (Table 3).

From the above results, excellent skin hygroscopicity of the extract of Asparagus can be confirmed, and the excellent moisturizing effect of the present invention can be obtained therefrom.

 [Preparation Example 1: Preparation of external preparation for skin containing asparagus extract]

In this Example, an external preparation for skin containing the extract of Asparagus obtained in Example 1 was prepared.

The prepared external preparation for skin was in cream form and its composition was as shown in Table 4 below. First, the component A recorded in the following Table 4 was heated and stored at 70 占 폚. Component B was added, preliminarily emulsified, uniformly emulsified with a homomixer, and gradually cooled to prepare an external preparation cream.

division ingredient
Production Example 1
(Unit: wt%) A


Asparagus extract
(Example 1) 5.0 1,3-butylene glycol - EDTA-2Na 0.02 Purified water to 100 B







Cetostearyl alcohol 2.0 Glyceryl stearate 1.5 Microcrystalline 0.7 Squalene 5.0 Liquid paraffin 3.0 Polysorbate 1.2 Sorbitan stearate 0.5 Tocopheryl acetate 0.2 Cyclomethicone 3.0 BHT 0.05

[Comparative Example 1: Preparation of composition for external application for skin without asparagus extract]

In this Comparative Example, the preparation process was the same as that of Experimental Example 4 except that asparagus extract was not included, and the preparation was carried out according to the following Table 5.

division ingredient
Comparative Example 1
(Unit: wt%) A


Asparagus extract
(Example 1) - 1,3-butylene glycol 5.0 EDTA-2Na 0.02 Purified water to 100 B







Cetostearyl alcohol 2.0 Glyceryl stearate 1.5 Microcrystalline 0.7 Squalene 5.0 Liquid paraffin 3.0 Polysorbate 1.2 Sorbitan stearate 0.5 Tocopheryl acetate 0.2 Cyclomethicone 3.0 BHT 0.05

[Experimental Example 4: Measurement of skin elasticity improvement effect of composition for external application]

This experiment was conducted to measure the skin elasticity improvement effect of the external preparation for skin prepared in Preparation Example 1 and Comparative Example 1.

Twenty subjects (20 to 35 year old female) were applied to the skin stretch zone for 2 consecutive months for 2 consecutive days of Manufacturing Example 1 and Comparative Example 1, respectively. To evaluate the effect of skin elasticity improvement, the skin elasticity was measured before use and after 2 months of use with a skin elasticity meter (SEM 575, C + K Electronic Co., Germany).

The experimental results are shown in Table 6 below as the R7 value of the skin elasticity measuring device. The R7 value indicates the viscoelasticity property of the skin (n = 10, p < 0.05).

Excellent elasticity enhancement effect A little bit of elasticity enhancement effect No elasticity enhancement effect Effective rate (%) Production Example 1 14 11 6 83.3% Comparative Example 1 3 5 22 26.7%

As a result of the experiment, it was confirmed that the skin elasticity improving effect of the applicant of Preparation Example 1 containing asparagus extract was superior to that of Comparative Example 1 (Table 6). From the above results, it was confirmed that the skin external-application composition of the present invention has an excellent skin elasticity-improving effect.

Claims (10)

Asparagus officinalis ) extract as an active ingredient.
The method according to claim 1,
In the cosmetic composition,
Wherein the cosmetic composition is one selected from the group consisting of lotion, gel, water-soluble liquid, cream, essence, makeup base of underwater type or water-in-oil type, foundation, skin cover, face powder and two-way cake.
The method according to claim 1,
The asparagus extract,
(a) adding asparagus a solvent selected from the group consisting of water or a lower alcohol (C1-C4) alcohol containing methanol or ethanol or a mixed solvent thereof, and extracting the mixture at a temperature of 10 to 30 ° C;
(b) filtering the resulting extract, followed by concentration and lyophilization; And
(c) adding to the concentrate or lyophilized product obtained after the step (b), at least one solvent selected from purified water, ethanol, butylene glycol and propylene glycol is added and extracted, Wherein the cosmetic composition for prevention or improvement of swelling filament is provided.
The method of claim 3,
The extraction of step (a)
Wherein the extraction method is any one of extraction method selected from cold extraction, reflux cooling extraction, hot water extraction, ultrasonic extraction and ultra high pressure extraction.
The method according to claim 1,
The asparagus extract,
The cosmetic composition for prevention or improvement of swollen skeletons according to claim 1, wherein the composition is added in an amount of 0.0001 to 90.0% by weight on a dry weight basis.
A composition for external application for skin for preventing or improving scarlet fever , which comprises asparagus ( Asparagus officinalis ) extract as an active ingredient.
The method according to claim 6,
The composition for external application for skin,
LIQUIDS AND SOLUTIONS, AEROSOLS, EXTRACTS, OINTMENTS, FLUIDEXTRACTS, LIQUIDS AND SOLUTIONS, LIQUIDS AND SOLUTIONS, Wherein the composition is any one selected from the group consisting of emulsions, suspensions, capsules, creams, soft or hard gelatine capsules, patches, and sustained release preparations. Skin external composition.
The method according to claim 6,
The asparagus extract,
(a) adding asparagus a solvent selected from the group consisting of water or a lower alcohol (C1-C4) alcohol containing methanol or ethanol or a mixed solvent thereof, and extracting the mixture at a temperature of 10 to 30 ° C;
(b) filtering the resulting extract, followed by concentration or lyophilization; And
(c) extracting the concentrate or lyophilized product obtained after the step (b) by adding at least one solvent selected from purified water, ethanol, butylene glycol and propylene glycol, and Wherein the composition for external application for skin is used for preventing or ameliorating swelling filament.
9. The method of claim 8,
The extraction of step (a)
Wherein the extracting method is any one extraction method selected from the group consisting of cold extraction, reflux cooling extraction, hot water extraction, ultrasonic extraction and ultrahigh pressure extraction.
The method according to claim 6,
The asparagus extract,
Wherein the composition is added in an amount of 0.0001 to 90.0% by weight based on the dry weight of the whole composition.