KR20100130877A - Composition for reducing irritation and inflammation in skin - Google Patents

Abstract

PURPOSE: A composition containing natural extract with effects of relieving skin inflammation and irritation is provided to treat skin irritation and to suppress nitrogen oxide, histamine, beta-hexosaminidase secretion. CONSTITUTION: A cosmetic composition with an effect of relieving skin inflammation and irritation contains 30-70 weight parts of Cymbidium lancifolium, 5-15 weight parts of Gentiana lutea, 5-10 weight parts of Malva sylvestris, 1-10 weight parts of Origanum vulgare and 20-40 weight parts of Crataegus oxyacantha extract as an active ingredient. The extract is isolated using water, low glycol o 1-6 carbon atoms, low alcohol of 1-4 carbon atoms or mixture thereof.

Description

Composition for reducing irritation and inflammation in skin

The present invention relates to a composition having a skin inflammation and irritation relief effect.

The skin is an organ covering the entire surface of the body, and protects the human body from external environment and irritation, and serves to suppress evaporation of moisture in the body. Therefore, the skin can only be exposed directly to the external environment.

The activity of the cells constituting the skin and the ability to protect the skin against external infectious agents decrease as the ozone layer breaks down, increasing ultraviolet rays reaching the surface, weakening immunity due to stress, and increasing exposure to pollutants such as harmful chemicals. Accordingly, the skin is sensitive to external stimuli, causing skin irritation and inflammatory reactions such as erythema, edema, and itching. In addition, the substances produced during the inflammatory reaction concomitantly cause pigmentation of the skin and accelerate the breakdown of skin elastic fibers and collagen fibers, thus affecting the increase of skin wrinkles.

In addition, many people, regardless of age or gender, use cosmetics in modern society, and the use of cosmetics may cause skin irritation, inflammation, itching and allergies. In general, a variety of raw materials are used in the manufacture of cosmetics, and typically dozens of raw materials are used. In particular, functional ingredients such as retinoids and fruit acids, and surfactants added for formulation are the main substances causing skin irritation. In order to reduce their side effects, a method of limiting the concentration or neutralizing acidity by adding strong alkali such as NaOH or KOH has been proposed, but there is a problem in that the effectiveness of the functional raw material is lowered.

In recent years, functional cosmetics that can cause more skin irritation are increasing, and cosmetic manufacturers are making efforts to minimize the irritation of cosmetic raw materials by applying commonly used irritant substances to the product. Therefore, it is necessary to select a substance that can reduce side effects caused by cosmetics and alleviate skin irritation while maintaining the effects of the functional raw materials.

Skin irritation that may occur with the use of cosmetics includes irritation such as itching, stinging and burning and inflammatory irritation such as erythema and edema. Such skin irritation may be said that cosmetics penetrate the skin and interact with the epidermis or dermal cells to activate the immune system and cause irritation and inflammatory reactions. In addition, contact dermatitis may be induced as a local inflammatory reaction occurring on the skin by an external irritant, and such contact dermatitis includes irritant contact dermatitis, allergic contact dermatitis, sensory irritation, and phototoxic dermatitis.

Inflammation is a protective action of biological tissues against foreign substances, and physiological reactions such as activation of inflammation mediators and immune cells present in local blood vessels or body fluids, secretion of inflammatory mediators, fluid infiltration, immune cell migration, and tissue destruction. External symptoms such as erythema, edema, fever, and pain. The main causes of inflammatory reactions are microbial infections, hypersensitivity reactions, physical factors, chemical factors and tissue necrosis. In particular, nitric oxide (NO) produced by nitric oxide synthase (NOS) and histamine and β-hexosaminidase secreted from mast cells are inflammatory reactions. Is a major transporter. Therefore, substances capable of inhibiting the release of histamine and β-hexosaminidase secreted from mast cells or reducing the production of nitric oxide are suitable for alleviating dermatitis such as atopic dermatitis as well as general inflammatory reactions. Efficacy may be provided.

The inventors confirm that the extracts of Gentian, Mallow, Oregano and Hawthorn mitigate skin irritation caused by sodium lauryl sulfate, and the cosmetic composition for alleviating the skin irritation comprising these extracts is registered in Korea Patent No. 10-0782517 I was registered as a ho.

In addition, the inventors of the present invention have conducted a multi-faceted study on the composition that can improve skin inflammation based on the extract, and when using the porcine egg extract with the extract, not only alleviates skin irritation, but also nitric oxide, The present invention has been found to be able to improve skin inflammation by inhibiting secretion of histamine and beta-hexosaminidase.

An object of the present invention to improve the skin irritation, to provide a cosmetic composition having a skin inflammation and irritation-reducing effect that can alleviate skin inflammation by inhibiting the release of nitric oxide, histamine, beta-hexosaminidase associated with the inflammatory response It is.

Another object of the present invention is to provide an external skin pharmaceutical composition for the prevention and treatment of skin inflammation and allergy having an inhibitory effect of inflammation-inducing substances.

In order to achieve the above object, the present invention provides a cosmetic composition having a skin inflammation and irritation-relieving effect comprising the extract of bamboo porridge, gentian, mallow, oregano and hawthorn as an active ingredient.

In addition, the present invention provides an external skin pharmaceutical composition for the prevention and treatment of skin inflammation and allergy, including the extract of bamboo porridge, gentian, mallow, oregano and hawthorn as an active ingredient.

The composition of the present invention exhibits anti-inflammatory activity by alleviating skin irritation such as inflammation, allergy, edema, erythema and inhibiting the secretion of inflammation-causing substances nitric oxide, histamine, beta-hexosaminidase, It may be used as a cosmetic composition for alleviating inflammation or an external dermal pharmaceutical composition for treating and preventing skin inflammation and allergy.

As used throughout this specification, 'skin inflammation' refers to various dermatitis in general, and includes allergic dermatitis, atopic dermatitis, and contact dermatitis.

The cosmetic composition of the present invention comprises an extract of Cymbidium lancifolium , Gentiana lutea , Malva sylvestris , Oregano vulgare and Crataegus oxyacantha as active ingredients. At this time, the extract is obtained by extracting from various organs or parts of each plant, it is possible to extract all, such as leaves, flowers, roots, stems, branches, shells and seeds.

Cymbidium lancifolium is long oval with long leaves, dark green, 20-30cm long, 2-3cm long, serrated at the edges. The tip is pointed and the petiole is long. Its node and leaf are similar to bamboo, called Jukbaek, and are distributed in Hallasan, Jeju Island. All parts of bamboo white eggs can be used, it is preferable to use leaves.

Gentiana lutea is a perennial plant with stems and ellipsoidal leaves, approximately 1.8 m in size, and preferably partially dried roots or roots are used. The main components are polyphenols, polysaccharides, genthiopicrosides, amarogentine, xanthone and the like.

Malva sylvestris is native to Asia and is 60-90 cm high. Leaves are alternate, rounded, but divided into 5-9, with small sawtooth at edges. Leaves, roots and flower parts can be used, of which it is preferable to use flower parts. The main ingredients are anthocyanins (malbin, malvidin), flavonoids, tannins, vitamins A, B1, B2, C, E and the like.

Origanum ( Origanum vulgare ) is a perennial plant with branches and is about 0.9m in size. Dried plants and leaves can be used, and among them, it is preferable to use leaves. Oregano extract is widely used as a food additive in bread production and the like due to the good aroma during bread production and the inhibitory effect of bacteria activity. The main ingredients are known flavonoids, caffeic acid derivatives, tannins and the like.

The hawthorn ( Crataegus oxyacantha ) is known for vasodilating, coercive, cardiovascular or digestive, pneumatic, branch office, analgesic and soothing. Partially dried fruit is preferably used, the fruit contains ursolic acid, oleanolic acid, rutin, and the like, the seed contains amigdaline, hyperin, fatty oil, and the bark contains aspirin.

Extract, which is an active ingredient of the cosmetic composition of the present invention, according to a conventional method known in the art, that is, using a conventional solvent, hot water extraction, room temperature extraction, warming extraction, ultrasonic extraction, supercritical extraction that are commonly used It can be produced by such a method.

Preferably it is extracted using a solvent selected from the group consisting of water, lower glycols having 1 to 6 carbon atoms, lower alcohols having 1 to 4 carbon atoms, and mixtures thereof. Most preferably, 1,3-butylene glycol is used as the extraction solvent, and extracted with an ultrasonic extractor at room temperature for 1 to 12 hours. The time of the ultrasonic extraction may vary depending on the amount of the sample to be extracted, and may undergo a process of filtering or purifying the extracted result.

In the composition of the present invention, the content of the extract is 0.001 to 30.0% by weight, preferably 0.1 to 5.0% by weight, based on the total weight of the cosmetic composition. If the content of the extract is less than the above range, there is a problem in showing the effect, on the contrary, if it exceeds the above range, there is no apparent increase in effect due to the increase in content, and there is a problem in that the stability of the formulation is lowered.

Preferably, the present invention is an extract extracted from the mixed raw material consisting of 30 to 70 parts by weight of bamboo porridge, 5 to 15 parts by weight of gentian, 5 to 10 parts by weight of mallow, 1 to 10 parts by weight of oregano and 20 to 40 parts by weight of hawthorn It includes. In addition, preferably the cosmetic composition of the present invention is 30 to 70 parts by weight of porridge extract, 5 to 15 parts by weight of gentian extract, 5 to 10 parts by weight of mallow extract, 1 to 10 parts by weight of oregano extract and 20 to 20 hawthorn extracts. 40 parts by weight. Such a mixing ratio is a range that can maximize skin irritation and inflammation relief of the cosmetic composition of the present invention.

The cosmetic composition of the present invention may be prepared in any formulation as is known and may be prepared as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion Formulations, such as foundation, wax foundation, spray, and the like.

In addition, the compositions of each formulation may contain various bases and additives necessary and appropriate for the formulation of the formulation, and nonionic surfactants, silicone polymers, extender pigments, fragrances, preservatives, and fungicides within the scope of not impairing the effect thereof. , Oxidative stabilizers, organic solvents, ionic or nonionic thickeners, softeners, antioxidants, free radical destroyers, opacifiers, stabilizers, emollients, silicones, α-hydroxy acids, antifoams, humectants, vitamins And known compounds such as insect repellents, fragrances, preservatives, surfactants, anti-inflammatory agents, substance P antagonists, fillers, polymers, propellants, basicizing or acidifying agents, or colorants.

Plant mixed extract of the present invention exhibited the ability to alleviate cytotoxicity by lactic acid, which is a commonly used stimulus (see Experimental Example 1), and inhibit the secretion of inflammation-causing substances (see Experimental Examples 2 to 4). In addition, the human patch test results were excellent skin irritation effect (see Experimental Example 6).

Since the extract of bamboo porridge, gentian, mallow, oregano and hawthorn of the present invention inhibits the secretion of inflammation-causing substances, it can be applied as an external skin pharmaceutical composition for the prevention and treatment of skin inflammation and allergy.

The content of the extract is 0.001 to 30% by weight, preferably 0.1 to 5.0% by weight based on the total weight of the skin external pharmaceutical composition. If the content of the extract is less than the above range, there is a problem in showing the effect, on the contrary, if it exceeds the above range, there is no apparent increase in effect due to the increase in content, and there is a problem in that the stability of the formulation is lowered.

The external skin pharmaceutical composition of the present invention is a skin external preparation for alleviating inflammation and allergy of the skin, and is used in the form of a skin external preparation such as cream, gel, patch, spray, ointment, warning agent, lotion agent, liniment agent, pasta agent or cataplasma agent. It can be prepared and used as a pharmaceutical composition, but is not limited thereto.

The dosage of the pharmaceutical composition of the present invention may include the type of disease, the severity of the disease, the type and amount of the active ingredient and other ingredients contained in the composition, the type of dosage form and the age, weight, general state of health, sex and diet, and time of administration of the patient. It can be adjusted according to various factors, including the route of administration and the rate of release of the composition, the duration of treatment, and the drug used simultaneously. However, for the desired effect, the composition of the present invention can be administered in an adult dose of 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg extract per day. At this time, the administration may be administered once a day, may be divided into several times. In addition, the pharmaceutical composition of the present invention can be used alone or in combination with methods such as surgical surgery.

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the examples.

Preparation Example 1 Preparation of Bamboo White Egg, Gentian, Mallow, Oregano and Hawthorn Mixed Extract

50 g of bamboo white egg, 10 g of gentian, 7.5 g of mallow, 2.5 g of oregano and 30 g of hawthorn are mixed and pulverized using a mixer. Then, 1,000 ml of 40% 1,3-butylene glycol aqueous solution is added and ultrasonically at room temperature. The extractor was extracted for 4 hours. After primary pressure filtration using a filter paper (Whatman Paper Filter No. 3), refrigerated for 2 days, and precipitates were produced to obtain a mixed extract through secondary pressure filtration.

Examples 1 to 3 and Comparative Example 1

The emulsion cosmetic compositions of Examples 1 to 3 and Comparative Example 1 were prepared according to a conventional method with the composition of Table 1.

ingredient
Content (% by weight) Example 1 Example 2 Example 3 Comparative Example 1 Mixed extract 2.0 5.0 10.0 - glycerin 5.0 5.0 5.0 5.0 Propylene glycol 4.0 4.0 4.0 4.0 Tocopheryl acetate 3.0 3.0 3.0 3.0 Liquid paraffin 5 5 5 5 Triethanolamine 1.0 1.0 1.0 1.0 Squalane 3.0 3.0 3.0 3.0 Macadamia Nut Oil 2.5 2.5 2.5 2.5 Polysorbate 60 1.6 1.6 1.6 1.6 Sorbitan sesquirrolate 1.6 1.6 1.6 1.6 Propylparaben 0.2 0.2 0.2 0.2 Carboxyl Vinyl Polymer 1.5 1.5 1.5 1.5 Purified water Balance Balance Balance Balance system 100 100 100 100

Experimental Example 1: Cytotoxicity Mitigation Effect Test

In order to confirm the cytotoxicity-reducing effect of the extract according to the present invention, cell death was induced by lactic acid, a skin stimulant, and the survival rate of the cells according to the addition of the extract was measured.

Fibroblasts (Cascade, UK) were incubated to about 80% growth in T-75 flasks (Nunc, United States). The cultured cells were transferred again to 2 × 10 ⁵ cells in each well of a 24-well plate (Nunc, USA) and incubated for 24 hours.

After incubation, the cells were completely attached and grown well under a microscope, and then experiments were performed using lactic acid, a skin cell stimulant. At this time, lactic acid was used at a concentration of 0.2% (w / v), which kills all skin cells. That is, 1 ml of DMEM (Sigma, United States) medium containing 0.2% lactic acid was added to each well of a 24-well plate, and the extract obtained in Preparation Example 1 was 0, 10, 50, 100, and 200 µg / Adjusted to mL concentration and added. At this time, all of the lactic acid and the extract was not added as a control.

After adding test substance, 200 μl of MTT ([3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) reagent was added to each well to compare the viability of the cells after 12 hours. . After 4 hours with the addition of the MTT reagent, the absorbance was measured at a wavelength of 570 nm using an ELISA Reader. Cell viability was calculated according to Equation 1 using the measured absorbance values, and the results are shown in Table 2 below.

% Cell viability = [test material absorbance / control absorbance] × 100

Mixed Extract Concentration (ug / ml) Control 0 10 50 100 200 Cell survival rate (%) 100 0 44 58 78 91

As shown in Table 2, as the concentration of the extract according to the present invention was increased, the cell viability increased, and the extract of the present invention inhibited cell death by lactic acid, thereby showing a cytotoxic alleviation effect. there was.

Experimental Example 2: Confirmation of NO secretion inhibitory effect

In order to evaluate the skin inflammation alleviating effect of the extract according to the present invention, the change in secretion amount of nitric oxide (NO) was measured.

Raw 264.7 cells, which are macrophages of mice, were added to Dulbecco's Modified Eagle's Medium (DMEM) medium by adding 10% FBS (fetal bovine serum), 100 units / mL penicillin and 100 μg / mL streptomycin. Incubated at 37 ° C., 5% CO ₂ ).

In order to induce an inflammatory response of the cells when the cells grew to 80% of the bottom of 100 mm dish, lipopolysaccharide was added to the DMEM culture medium containing 1% FBS at a concentration of 100 ng / mL.

After culturing for 24 hours by adding the extract or lipopolysaccharide obtained in Preparation Example 1 to Raw 264.7 cells, the culture solution was collected and the NO secretion was measured by a grease reagent system ( Kim JK et al., Biochem Biophys Res Commun., 345 , pp 1215-1223, 2006 ), the results obtained are shown in Table 3 below.

Mixed Extract Concentration (ug / ml) Control 10 50 100 200 NO secretion
(% Of control group secretion) 100 71 57 31 10

As shown in Table 3, when the extract of the present invention was treated, NO production induced by lipopolysaccharide was reduced in a concentration-dependent manner. These results are expected to be able to effectively reduce skin inflammation when using the extract of the present invention.

Experimental Example 3: Confirmation of the inhibitory effect of histamine secretion

In order to evaluate the skin inflammation relieving effect of the extract according to the present invention, the change in the amount of histamine secretion was measured.

Histamine is widely known as a mediator that mediates inflammatory responses, such as interleukin-2, substance P, opoid peptides, and prostaglandin E, mainly in mast cells It is released from mast cells and is known to cause itching and pain. Therefore, the histamine's ability to inhibit secretion can be used to suppress a series of inflammatory reactions such as inflammatory reactions, itching, and pain.

Rat basophil leukocytes, RBL-2H3 cells, were prepared in EAG (Eagles Minimum Essential Medium) medium with 10% fetal bovine serum (FBS), 1% glutamine, 100 units / mL penicillin and 100 μg / mL streptomycin. ) Was added and incubated in an incubator (37 ° C., 5% CO ₂ ).

Cells were harvested by trypsin / versene when grown to 80% of the bottom of a 100 mm dish and incubated for 18 hours by inoculating 2 × 10 ⁶ cells into 24 well plates. The medium was removed, incubated for 1 hour with EMEM medium containing IgE (0.2 ug / ml), and 0.2 ml of release buffer containing Anti-IgE was added for 10 minutes. The released histamine was quantified with a radioimmunoassay kit (Immunotech, Marseille, France) and the results obtained are shown in Table 4 below.

Mixed Extract Concentration (ug / ml) Control One 5 10 20 Histamine release
(% Of control group secretion) 100 88 61 47 37

As shown in Table 4, the extract treatment group of the present invention concentration-dependently inhibited histamine secretion of basophil leukocytes in rats. These results are expected to be able to effectively reduce skin inflammation when using the extract of the present invention.

Experimental Example 4: Confirmation of beta-hexosaminidase secretion inhibitory effect

In order to evaluate the skin inflammation relieving effect of the mixed extract according to the present invention, the change in secretion amount of beta-hexosaminidase was measured.

Beta-hexosaminidase (β-hexosaminidase) is an enzyme contained in mast cells, which are released together with histamine when mast cells are activated by an immune response. Thus, beta-hexosaminidase can be used as an indicator of degranulation of mast cells.

Rat basophil leukocytes, RBL-2H3 cells, were prepared in EAG (Eagles Minimum Essential Medium) medium with 10% fetal bovine serum (FBS), 1% glutamine, 100 units / mL penicillin and 100 μg / mL streptomycin. ) Was added and incubated in an incubator (37 ° C., 5% CO ₂ ).

Cells were harvested by trypsin / versene when grown to about 80% of the bottom of a 100 mm dish, and incubated for 18 hours with 2 X 10 ⁶ cells per well inoculated into 24 well plates. The medium was removed and the cells were sensitized by reacting the cells with 1 μg / ml of anti-DNP-IgE for 18 hours, and then the samples were pretreated for 1 hour. After 10 minutes of treatment with 10 μg / ml of DNP-BSA, the upper layer was obtained.

50 μl of the obtained supernatant and 50 μl of 5 mM p-nitrophenyl-N-acetyl-β-glucosamide dissolved in 0.2 M citrate buffer (pH 4.5) were reacted in an incubator for 30 minutes. 1N HCl was added to stop the reaction, and the release amount of beta-hexosaminidase was measured at 405 nm. The same experiment was performed with the supernatant obtained by hemolysis of cells with 0.1% Triton X-100 to determine the release of total beta-hexosaminidase.

Mixed Extract Concentration (ug / ml) Control 10 20 50 100 Beta-Hexosaminidase Secretion
(% Of control group secretion) 100 98 95 89 85

Referring to Table 5, the extract treatment group according to the present invention was confirmed to have an anti-allergic effect by concentration-dependently inhibiting the beta-hexosaminidase secretion of RBL-2H3 cells. These results are expected to effectively reduce skin inflammation and allergies when using the extract of the present invention.

Experimental Example 5: Stability test of emulsion formulation containing mixed extract

The stability with storage temperature of the cosmetic formulation of the present invention was tested. Examples 1 to 3 and Comparative Example 1 containing the mixed extracts were stored in an opaque glass container for 16 weeks in a constant temperature bath maintained at 45 ° C., and also kept in a constant shade at 4 ° C. and completely shaded in a refrigerator. After storage for 16 weeks, the degree of discoloration was measured. The results are shown in Table 7 below. At this time, the degree of discoloration of the product was evaluated by classifying into six grades of the following Table 6.

Product discoloration evaluation criteria score Evaluation standard 0 No change One Very little change 2 Little change 3 Slightly worse 4 Badly changed 5 Extremely severe

Storage period Discoloration degree of test substance 4 ℃ 45 ℃ Example 1 Example 2 Example 3 Comparative Example 1 Example 1 Example 2 Example 3 Comparative Example 1 Week 0 0 0 0 0 0 0 0 0 1 week 0 0 0 0 0 0 0 0 2 weeks 0 0 0 0 0 0 0 0 4 Weeks 0 0 0 0 0 0 0 0 8 Weeks 0 0 0 0 0 0 0 0 12 Weeks 0 0 0 0 0 0 0 0 16 Weeks 0 0 0 0 0 0 One 0

As can be seen from the results of Table 7, the discoloration does not occur in Comparative Example 1 that does not include the mixed extract, it can be seen that the discoloration does not occur by the other components of the emulsion, the mixed extract 10% Discoloration occurred slightly when stored for 16 weeks in the formulation of Example 3 containing, whereas discoloration did not occur when an emulsion containing less than 5% of the mixed extract was stored for 45 to 16 weeks. The formulations of Examples 1 and 2 containing 5% or less of mixed extracts were found to have excellent stability of the formulations as in Comparative Example 1, which did not contain extracts.

Experimental Example 6: Confirming the skin irritation relief effect

In order to evaluate the skin irritation efficacy of the mixed extract according to the present invention, the skin irritation induced by irritation of sodium dodecyl sulphate (SDS) and skin reactivity was measured.

First, an emulsion cosmetic was prepared with the composition shown in Table 8.

ingredient
Content (% by weight) Example 4
Comparative Example 2 Control Mixed extract 2.0 - - Sodium Dodecyl Sulfate 1.0 1.0 - glycerin 5.0 5.0 5.0 Propylene glycol 4.0 4.0 4.0 Tocopheryl acetate 3.0 3.0 3.0 Liquid paraffin 5 5 5 Triethanolamine 1.0 1.0 1.0 Squalane 3.0 3.0 3.0 Macadamia Nut Oil 2.5 2.5 2.5 Polysorbate 60 1.6 1.6 1.6 Sorbitan sesquirrolate 1.6 1.6 1.6 Propylparaben 0.2 0.2 0.2 Carboxyl Vinyl Polymer 1.5 1.5 1.5 Purified water Balance Balance Balance system 100 100 100

The emulsion formulations of Example 4 and Comparative Example 2 prepared as described above were compared to measure the skin irritation effect by the surfactant.

The subjects of this study were twenty women in their 20s and 30s who had no previous hypersensitivity to skin irritation and had no skin disease or skin allergy symptoms. The test site was first wiped with 70% ethanol and then dried. 15 μg of the prepared test substance was dropped in a Fin chamber (100 × 10, EPITEST, Finland), and then sealed in the forearm inner part of the test subject. The patch was applied for 24 hours, the patch was removed, and the test site was marked with a marker pen. After 1 hour and 24 hours (1 hour after removal of the patch, after 24 hours = 24 hours, 48 hours including patch attachment), the presence of erythema and edema at the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America). It was.

The skin reaction was determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG) (Table 8), and the average skin reactivity was calculated by Equation 2 below, and the results are shown in Table 9.

Symbol score Evaluation standard - 0.0 No response ± 0.5 Dim or light iris + 1.0 Boundary but weak erythema, edema and papules ++ 2.0 Pronounced erythema, papules and vesicles +++ 3.0 Severe erythema, alveolus, crust

 Mean skin responsiveness (mean score) = {(score × number of respondents) / (maximum score × total number of subjects)} × 100

division
24 hours 48 hours Average skin reactivity
(n = 20)
± + ++ +++ ± + ++ +++ Control - - - - - - - - 0.00 Example 4 2 - - - - - - - 0.83 Comparative Example 2 4 One - - 2 - - - 3.33

As can be seen in Table 10, the skin patch test showed a strong skin irritation for Comparative Example 2 containing sodium dodecyl sulfate, Example 4 containing the extract of the present invention showed a stimulating effect.

Hereinafter, a softening lotion, a convergent lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence and a pack are illustrated as a prescription example of the present invention, but the formulation of the cosmetic composition of the present invention is not limited thereto.

Formulation Example 1: Softener (Skin Lotion)

As shown in Table 11 below, the flexible makeup was prepared according to a conventional method.

ingredient Content (% by weight) Mixed extract 0.3 glycerin 5.0 1,3-butylene glycol 3.0 PEG 1500 1.0 Allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaruronate 5.0 ethanol 10.0 Octyldodec-16 0.2 Polysorbate 20 0.2 Uniside-U 13 0.01 Distilled water Balance Sum 100

Formulation Example 2: Converging Cosmetic Water

As shown in Table 12 below, the convergent makeup was prepared according to a conventional method.

ingredient Content (% by weight) Mixed extract 0.3 glycerin 2.0 1,3-butylene glycol 2.0 Allantoin 0.2 DL-Panthenol 0.2 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaruronate 3.0 ethanol 15.0 Polysorbate 20 0.3 Witch Hagel Extract 2.0 Citric acid a very small amount Uniside-U 13 0.01 Distilled water Balance Sum 100

Formulation Example 3: Nutrients

As shown in Table 13, nutrient lotion was prepared according to a conventional method.

ingredient Content (% by weight) Mixed extract 0.3 Glyceryl Stearate SE 1.5 Cetearyl Alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Cured Vegetable Oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoine 2.0 Dimethicone 0.8 Tocopherol Acetate 0.5 Carboxyvinyl Polymer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium hyaluronate 5.0 Triethanolamine 0.12 Uniside-U 13 0.02 Distilled water Balance Sum 100

Formulation Example 4: Nutrition Cream

As shown in Table 14, nutrition cream was prepared according to a conventional method.

ingredient Content (% by weight) Mixed extract 0.3 Lipophilic monostearate 2.0 Cetearyl Alcohol 2.2 Stearic acid 1.5 Beeswax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Cured Vegetable Oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoine 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 Uniside-Y13 0.02 Distilled water Balance Sum 100

Formulation Example 5: Massage Cream

Massage creams were prepared according to conventional methods as shown in Table 15 below.

ingredient Content (% by weight) Mixed extract 0.3 Lipophilic Monostearic Acid Glycerin 1.5 Cetearyl Alcohol 1.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl Isosterate 5.0 Squalane 5.0 Mineral oil 35.0 Dimethicone 0.5 Hydroxyethyl cellulose 0.12 glycerin 6.0 Triethanolamine 0.7 Uniside-Y13 0.02 Distilled water Balance Sum 100

Formulation Example 6: Essence

Essences were prepared according to conventional methods as shown in Table 16 below.

ingredient Content (% by weight) Mixed extract 0.3 glycerin 10.0 Betaine 5.0 PEG 1500 2.0 Allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethylcellulose 0.1 Sodium hyaruronate 8.0 Carboxy Vinyl Polymer 0.2 Triethanolamine 0.18 Octyldodecanol 0.3 Octyldodec-16 0.4 ethanol 6.0 Uniside-U 13 0.01 Distilled water Balance Sum 100

Formulation Example 7: Pack

Packs were prepared according to conventional methods as shown in Table 17 below.

ingredient Content (% by weight) Mixed extract 0.3 Polyvinyl alcohol 15.0 Cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 Chicdestrin 0.15 DL-Panthenol 0.4 Allantoin 0.1 Glycyrisin Monoammonium 0.3 Nicotinamide 0.5 ethanol 6.0 PEG 40 Cured Castor Oil 0.3 Uniside-Y13 0.02 Distilled water Balance Sum 100

Claims (8)

Cosmetics having skin irritation and irritation-reducing effects, including extracts of Cymbidium lancifolium , Gentiana lutea , Malva sylvestris , Origanum vulgare and Crataegus oxyacantha as active ingredients Composition. The method of claim 1, The extract is characterized in that it is extracted from a mixed raw material consisting of 30 to 70 parts by weight of bamboo porridge, 5 to 15 parts by weight of gentian, 5 to 10 parts by weight of mallow, 1 to 10 parts by weight of oregano, and 20 to 40 parts by weight of hawthorn. Cosmetic composition. The method of claim 1, The extract is a cosmetic composition, characterized in that extracted with one extraction solvent selected from the group consisting of water, lower glycol having 1 to 6 carbon atoms, lower alcohol having 1 to 4 carbon atoms and mixtures thereof. The method of claim 1, The content of the extract is a cosmetic composition, characterized in that 0.001 to 30% by weight relative to the total weight of the cosmetic composition. The method of claim 1, The composition is one or more selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. Cosmetic composition, characterized in that it has a formulation. For the prevention and treatment of skin inflammation and allergy, which contains extracts of Cymbidium lancifolium , Gentiana lutea , Malva sylvestris , Origanum vulgare and Crataegus oxyacantha as active ingredients External skin pharmaceutical composition. The method of claim 6, The content of the extract is an external skin pharmaceutical composition, characterized in that 0.001 to 30% by weight based on the total weight of the pharmaceutical composition. The method of claim 6, The composition is an external dermal pharmaceutical composition, characterized in that it has one formulation selected from the group consisting of creams, gels, patches, sprays, ointments, warnings, lotions, linen, pasta and cataplasma.