KR101382115B1 - Health food for improving atopic dermatitis and xerosis containing ginseng fruit extracts - Google Patents

Abstract

The present invention relates to a food composition for improving atopic dermatitis containing ginseng fruit extract, and more specifically, the amount of water loss through the epidermis by using ginseng fruit extract having a different ingredient and composition from ginseng root in the ground part of ginseng as an active ingredient (TEWL) : Food composition for improving atopic dermatitis or dryness in which the fatty layer structure of the skin epidermal barrier is damaged by reducing trans epidermal water loss and reducing pruritus.

Ginseng Berry Extract, Atopic Dermatitis, Water Loss (TEWL), Pruritus (Itching)

Description

Health food for improving atopic dermatitis and xerosis containing ginseng fruit extracts}

The present invention relates to a food composition for improving atopic dermatitis containing ginseng fruit extract, and more specifically, the amount of water loss through the epidermis by using ginseng fruit extract having a different ingredient and composition from ginseng root in the ground part of ginseng as an active ingredient (TEWL) : Food composition for improving atopic dermatitis or dryness in which the fatty layer structure of the skin epidermal barrier is damaged by reducing trans epidermal water loss and reducing pruritus.

Ginseng (Panax ginseng C.A. Meyer) is a plant belonging to the genus Ogapi and ginseng, which has been used in Korea, China, and Japan for more than 2,000 years, and has been used to prevent disease and extend life. The effects and effects of ginseng so far known are the effects on the central nervous system, anticarcinogenic activity, anticancer activity, immune function control effect, antidiabetic effect, hepatic antifungal effect, cardiovascular disorder improvement, anti arteriosclerosis action, blood pressure control action, Improvement of menopausal disorders, effects on osteoporosis, antistress, anti-fatigue, antioxidant activity and anti-aging effects (Korea Ginseng 'Ingredients and Efficacy', Korea Ginseng and Tobacco Research Institute, 56-112, 1996).

Ginsenoside, a representative physiologically active ingredient of ginseng, is distributed evenly in the ground and underground parts of ginseng, and in particular, ginsenoside content and composition differ depending on the parts such as ginseng root (root), ginseng leaf and ginseng fruit. (Attele AS et al, Biochem Pharmacol, 58; 1685-1693, 1999). In particular, ginseng fruit was reported to have better results than ginseng root in antidiabetic efficacy based on ginseng root (root) and other ginsenoside components and ingredients (Dey L. et al., Phytomedicine, 10; 600). -605, 2003).

On the other hand, ginseng fruit is considered to be more precious than ginseng since ancient times, and has been selected and harvested for the purpose of obtaining seeds. Ginseng fruit is only harvested once every 4 years during the cultivation period of ginseng. It is difficult to produce good seedlings because seed is too small when grown in 3 years, and seeds are faithful and good when harvested in 5 years or more. In addition to inhibiting development, the tissue is not dense, so red ginseng quality can be greatly reduced when manufacturing red ginseng. In addition, the yield and red ginseng quality is greatly degraded when the number of cultivation is collected more than two times during the cultivation period.

Meanwhile, the epidermis, the outermost layer of skin, serves to protect the human body from the outside. The stratum corneum, which is the uppermost layer of the epidermis, is the epidermal barrier. It serves as the first line of human defense mechanisms to prevent skin invasion such as viruses. The stratum corneum has a layered structure in which fats are located between the keratinocytes and the bricks and the plaster therebetween.

In terms of biochemistry, atopic dermatitis has been interpreted to show symptoms of dryness, hyperproliferation, and inflammation of the epidermis due to damage of the sebaceous layer of the sebaceous membrane and evaporation and infection through the epidermis. It has been reported that levels of moisturizing factors are reduced. Also, in the case of healthy skin, the level of unsaturated fatty acids and ceramides in epidermis decreases with winter and aging with decreasing humidity and temperature, indicating that drying of the epidermis is inversely correlated with changes in sebum and unsaturated fatty acid content of sebaceous membrane. Able to know.

The Atopic Dermatitis Therapeutics Market surpassed US $ 560 million in 1999, combined with US, UK, France, Germany, Italy, Spain and Japan, and is expected to reach US $ 1.2 billion in 2009. Currently, the foreign market is also led by topical corticosteroid preparations, and vitamin D external preparations, anthralin, tar preparations, oral antihistamines, and second-generation retinoids are used.

Cyclosporine and methotrexate have been used as treatments for immune suppression, but sales are not high. However, future market growth is expected to be driven by immunosuppressive drugs such as tacrolimus (protocol) and pimecrolimus (Elidel), which are expected to replace conventional topical corticosteroids. In 2002, the potential market for atopy products (pharmaceuticals + cosmetics) is estimated at W100bn, of which steroid drugs are the largest, at W28bn.

In Korea, atopic dermatitis is one of the skin diseases that cause skin dryness, and it is a representative skin disease that occurs in about 10-20% of the total population. The atopic dermatitis market is growing at a rate of over 50% every year, and the market is attracting attention from venture companies, pharmaceutical companies, and cosmetics companies. In addition, the distribution market of cosmetics and pharmaceuticals, such as discount stores and pharmacies is limited, but one of the characteristics is that the distribution channels such as pharmacy medical institutions and cosmetics specialists are diverse. The cosmetics market in the atopic dermatitis market continued to grow since 1998, to 15 billion won in 2001 and 25 billion won in 2002 and 40 billion won in 2003.

<2003 Atopic Patient Count and Prescription Count Survey>

Classification September 2002 to February 2003 September 2002 to September 2003 The number of hospital diagnosis patients 1,386,000 2,827,000 The number of hospital treatment patients 1,230,000 2,478,000 Drug prices for hospital care 2.82 2.85

<Atopic Patients by Sex / Age>

0-1 years old 1-4 years old 5-11 years old 12-19 years old 20-29 years 30-39 years old 40-54 years old 55-64 years old 65 years old or older Sum 2002/9 ~
2003/2 52000 236000 166000 75000 58000 48000 31000 25000 27000 724000 % 4.2 17.5 12.3 5.5 4.4 3.6 2.3 1.8 2.0 53.8 2002/9 ~
2003/9 150000 515000 347000 145000 107000 78000 45000 32000 40000 1460000 % 5.9 18.7 12.6 5.3 3.9 2.8 1.8 1.2 1.5 52.9

(man)

0-1 years old 1-4 years old 5-11 years old 12-19 years old 20-29 years 30-39 years old 40-54 years old 55-64 years old 65 years old or older Sum 2002/9 ~
2003/2 33000 212000 131000 53000 42000 22000 54000 15000 20000 622000 % 2.4 19.8 9.7 6.5 3.1 1.7 4.0 1.2 1.5 48.2 2002/9 ~
2003/9 105000 451000 274000 187000 113000 50000 88000 23000 28000 1299000 % 3.8 18.3 9.5 6.1 4.1 1.8 3.2 0.8 1.0 47.1

(Woman)

0-1 years old 1-4 years old 5-11 years old 12-19 years old 20-29 years 30-39 years old 40-54 years old 55-64 years old 65 years old or older Sum 2002/9 ~
2003/2 9000 448000 297000 157000 101000 70000 85000 41000 47000 1346000 % 6.7 33.3 22.0 12.4 7.5 5.2 6.3 3.0 3.5 100 2002/9 ~
2003/9 256000 986000 521000 313000 220000 128000 132000 55000 68000 2759000 % 9.3 35.0 22.5 11.3 8.0 4.7 4.8 2.0 2.5 100

(Sum)

In addition to reporting reduced levels of ceramide-unsaturated fatty acids and natural moisturizing factors in the epidermis of atopic dermatitis patients, specific nutrients, especially in response to changes in the public's perception that skin's dryness prevention and soft, moist feeling are closely related to nutrition in terms of skin health Although studies on sebum membrane maintenance and moisturizing function of unsaturated fatty acids are conducted in the US and Europe, studies on the development and production of health functional food materials for enhancing skin moisturization are insufficient in Korea.

Topical steroids, antihistamines, and ultraviolet light therapy are used as current treatments for atopic dermatitis, but these treatments have several side effects, are difficult to expect fundamental healing, and prevent the deterioration of inflammation and prolong the remission period until recurrence. Since it is only meaningful, it is urgent to develop a therapeutic agent that can be used for a long time without any side effects. In addition, moisturizing lotions with ceramides have recently been preferred, but this also requires frequent application and shows temporary relief of temporary drying symptoms. Therefore, it is urgent to develop a therapeutic agent that can be used for a long time without side effects. .

Thus, the present inventors while researching to find a natural material that improves atopic dermatitis by using ginseng fruit, which is the ground part of ginseng, in addition to the ginseng root which is the underground part of ginseng, the ginseng fruit extract is different from general ginseng root and red ginseng by atopy It has been found that there is an improvement in dermatitis and dryness, and the present invention has been completed.

Accordingly, it is an object of the present invention to provide a food composition for improving atopic dermatitis containing ginseng fruit extract as an active ingredient.

Another object of the present invention to provide a food composition for improving the skin itching symptoms containing ginseng fruit extract as an active ingredient.

In order to achieve the above object, the present invention provides a food composition for improving atopic dermatitis containing ginseng fruit extract as an active ingredient.

In another aspect, the present invention provides a food composition for improving skin itching symptoms containing ginseng fruit extract as an active ingredient.

In the present invention, ginseng fruit extract may be mixed in an amount of 0.01 to 100% by weight based on the total weight of the composition according to the form of health food.

Food composition for improving atopic dermatitis containing ginseng fruit extract according to the present invention inhibited the epidermal water loss (TEWL) of the epidermis and at the same time showed an excellent effect of improving atopic dermatitis by improving pruritus (itch). .

In the present invention, the ginseng fruit extract is prepared by adding water or ethanol to the ginseng fruit dry raw material obtained by sun drying or hot air drying to remove the flesh and skin peeled by separating the seeds of the ginseng fruit.

Hereinafter, the present invention will be described in more detail with reference to examples. It will be apparent, however, to those skilled in the art that these embodiments are for describing the present invention and that the scope of the present invention is not limited to these embodiments.

[Example 1] Preparation of ginseng fruit extract

1) Ginseng fruit pretreatment: The raw ginseng fruit was harvested and the seeds were separated and removed. Then, the ginseng fruit and the skin of the ginseng fruit were dried with sunlight or hot air to prepare ginseng fruit raw material.

2) Preparation of ginseng fruit extract: 1 g of dried ginseng fruit extract was added to 3 L of ethanol to extract reflux, and then filtered and concentrated under reduced pressure at 40 ~ 45 ℃ to obtain 300 g of ginseng fruit extract.

[Comparative Example 1] Production of ginseng muscle root extract

Example 1 was prepared in the same manner as in Example 1, except that ginseng root (root) was used instead of ginseng fruit.

[Test Example 1] Comparison of components of ginseng fruit extract

<Analysis of Ginsenoside (Ginseng Saponin) Components of Ginseng Fruit and Ginseng Root>

In Example 1 and Comparative Example 1, respectively, ginseng fruit and ginseng root extract were prepared, and then, the extracts were treated with ether to remove fat-soluble components, followed by extracting and concentrating ginsponin with butanol (BuOH). Cenoside component analysis was carried out, and the results are shown in FIGS. 1 and 5.

Example 1 Comparative Example 1 Crude saponin content
(Dry weight)
33.42%
16.70% PD / PT ratio 0.73 3.23

The ginseng fruit extract prepared in Example 1 had about twice the crude ginsenoside content of the ginseng root extract prepared in Comparative Example 1, and the ginsenoside was divided into PD (protopanaxadiol) -ginsenoside Rb1, Rb2 , Rc and Rd "and PT (Protopanaxatriol) system -" Ginsenoside Re, Rg1 and Rg2 ", the ginseng fruit and ginseng root showed distinct differences and characteristics in the composition of 0.73 and 3.23 respectively .

<Mineral composition analysis of ginseng fruit extract>

In order to distinguish the ginseng fruit extract prepared in Example 1 has the characteristics of 'fruit' different from ginseng, mineral components including vitamins were analyzed, and the results are shown in Table 6 below.

ingredient content ingredient content Potassium (mg / 100g) 5865.57 Magnesium (mg / 100 g) 354.38 Calcium (mg / 100g) 819.26 Zinc (mg / 100g) 178.49 Iron (mg / 100g) 59.31 Vitamin A (μg / 100 g, RE) 213.11 Phosphorus (mg / 100 g) 187.17 Vitamin B1 (mg / 100g) 12.29 Vitamin B2 (mg / 100g) 8.45 Vitamin B6 (mg / 100g)  10.50 Vitamin C (mg / 100g)  4.91 Vitamin E (mg / 100 g, α-TE) 23.61 Vitamin K (μg / 100g) 232.12 Niacin (mg / 100 g, NE) 5.76 Pantothenic acid (mg / 100g) 5.87 Folic acid (μg / 100g)  349.97

As described above, the constituent characteristics of the ginseng fruit used in the present invention is that the ginseng saponin is contained in a larger amount than that of the ginseng root, and the saponin composition properties are opposite to each other. Also, unlike the ginseng root, the content of 16 kinds of vitamins and minerals is rich , And based on this, the following experiments on skin efficacy were conducted.

Comparative Example 2 Preparation of Red Ginseng Extract

6 kg of ethanol was added to 1 kg of red ginseng powder, and the mixture was extracted three times at room temperature and refluxed. The extract was filtered using a conventional method and concentrated using a reduced pressure concentrator to obtain 250 g of red ginseng extract.

[Test Example 2] Atopic dermatitis and dryness improvement effect in vivo

Experimental Animal

Atopy mice (NC / Nga Tnd mice) and normal control mice were maintained at a temperature of 22 ± 1 ° C. and a humidity of 60 ± 5% and adapted to varying contrast at 12 hour intervals.

Dietary Composition

Adapted to a solid blended feed for 1 week before breeding into the experimental diet and divided into egg mass according to body weight, the experimental diet was fed to NC / Nga Tnd mice for 10 weeks. The dietary composition of each experimental group is shown in Table 7 below, and the red ginseng extract of Comparative Example 2 and the ginseng fruit extract of Example 1 were supplied with a mixture of raw materials corresponding to 5% of the total diet weight. Therefore, the experimental group was divided into the normal control group, atopic dermatitis group and red ginseng extract-containing diet atopic dermatitis group (Comparative Example 2 containing group) and ginseng fruit extract containing dietary atopic dermatitis group (Example 1 containing group).

Diet composition (g / kg dry diet) ingredient Experimental group Normal control group
(n = 10) Atopic dermatitis
(n = 10) Contains red ginseng extract
Dietary Atopy
Trigger group (n = 10) Contains ginseng fruit extract
Dietary Atopy
Trigger group (n = 10) casein 230 230 230 230 L-cystine 3 3 3 3 Corn oil 100 100 100 100 cellulose 50 50 50 50 Vitamin mix 10 10 10 10 Mineral mix 35 35 35 35 Sucrose 200 200 200 200 Corn starch 372 372 354.5 354.5 Sample - - 17.5 17.5

-Normal control group : NC / Nga Tnd mouse fed normal diet

-Atopic Dermatitis Group: NC / Nga Tnd Mouse

Dietary atopic induction group containing red ginseng extract: NC / Nga Tnd mouse + red ginseng extract

-Dietary Atopic Dermal Group Containing Ginseng Fruit Extract: NC / Nga Tnd Mouse + Ginseng Fruit Extract

Dietary efficiency measurement

To determine dietary efficiency, the weight of each rat, the amount of food remaining and the amount of food supplied were measured twice a week.

<Measurement of Trans Epidermal Water Loss (TEWL)>

It was examined how the epidermal water loss (TEWL) of epidermis was affected by the ginseng fruit extract of Example 1 and the red ginseng extract of Comparative Example 2.

After shaving the back part of the experimental animal and leaving it for 30 minutes in a space where constant temperature and humidity were maintained, the numerical value that was lightly pressed by touching the hydrometer's probe in close contact with the back skin surface was recorded. 2 is shown.

The moisture content of the stratum corneum is determined by the sebaceous membrane, the lipid mixture produced and secreted by the epidermis, and the natural moisturizing factor (NMF), a water-soluble component present in the stratum corneum.

If the production of sebaceous membranes and natural moisturizing factors decreases due to changes in endogenous factors such as climate, seasons, environmental factors such as UV, genetics, aging, hormones, immunity, stress, and nutritional status, the epidermal moisture loss (TEWL) Increased water loss causes skin to dry, and the surface becomes dry and dull, resulting in dead skin cells.

In the results of Figure 2, after measuring the epidermal loss of each group after a 10-week diet, the highest in the atopic dermatitis group, atopic dermatitis control group, the lowest in the normal group control group, the diet containing red ginseng extract of Comparative Example 2 The ginseng fruit extract diet of Example 1 than the atopic dermatitis group (Comparative Example 2 containing group) suppresses the epidermal moisture loss to the normal control group level compared to the red ginseng extract of Comparative Example 2 Ginseng fruit extract was found to be excellent in inhibiting the epidermal moisture loss (TEWL).

Measurement of general clinical symptoms of atopic dermatitis: behavioral analysis of itching

Five groups in each group were photographed using an automatic video camera for 20 minutes each week, and the number of scratches of the whole body including the face, ear and neck was measured and recorded. Since the types of pruritus of atopy-induced NC / Nga mice vary among individuals, the significance of the experimental groups was measured by measuring only the number of scratches of each subject per unit time (20 minutes), regardless of the scratching intensity and the scratching duration. Analytically, the results are shown in FIG. 3.

In the results of Figure 3, NC in the atopic dermatitis animal model with general symptoms of atopic dermatitis, including pruritus (itch) increase in the dietary atopic dermatitis group (Example 1 containing group) of the ginseng fruit extract of Example 1 according to the present invention Scratching number in / Nga mice was significantly reduced compared to the atopic dermatitis group (Comparative Example 2 containing group) containing red ginseng extract of Comparative Example 2. This result shows that the ginseng fruit extract of Example 1 according to the present invention improves pruritus (itch).

FIG. 1 is a graph showing ginsenoside components of the ginseng fruit extract of Example 1 and the ginseng extract of Comparative Example 1. FIG.

Figure 2 is a graph showing the amount of water (Trans epidermal water loss) through the epidermis for the test substance.

3 is a graph measuring the scratching number (count / 20min) to determine the effect of the dietary supply of the test substance on the scratching number (Values are mean ± SD (n = 10), means with different letters differ P <0.05).

Claims (3)

Food composition for improving the skin itching symptoms containing ginseng fruit extract as an active ingredient. delete The food composition according to claim 1, wherein the ginseng fruit extract is prepared by adding water or ethanol to the ginseng fruit dry raw material obtained by sun drying or hot air drying the flesh and skin of the ginseng fruit.

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