KR100975426B1 - Composition comprising the crude extract for preventing and treating allergic skin diseases - Google Patents

Abstract

The present invention relates to a composition for the prevention and treatment of allergic skin diseases using herbal extracts, and more particularly, the animal of which macrophage extract or macrophage, spermatozoon, ringworm skin, shepherdum and baekryeok complex extract causes atopic dermatitis The number of scratches in the model is excellently reduced, and the sensory test has an effect of lowering the atopic dermatitis score, and it is very effective for allergic skin diseases by confirming the effect of reducing the elevation of IgE and IL4 in the blood according to the expression of atopic dermatitis. Effective herbal extracts and uses thereof.

Herbal Extract, Allergic Skin Disease

Description

Composition comprising the crude extract for preventing and treating allergic skin diseases}

The present invention relates to a composition for preventing and treating skin diseases using herbal extracts.

Allergic skin diseases include anaphylaxis, allergic rhinitis, asthma, atopic dermatitis and urticaria, and the prevalence is increasing [Wuthrich B. Int. Arch. Allergy Appl. Immunol ., 90, pp 3-10, 1989]. Generally allergic diseases are mediated by IgE, which involves Type 2 helper T cells (Th2), mast cells and eosinophils.

The allergic reaction mechanism is divided into four types, type 1 to type 4, type 1 is called immediate type and type 4 is called delay type. Atopic dermatitis is considered to be a dermatitis involving allergic reactions of type 1 and type 4 (Japanese Patent Publication No. 2000-119127). Allergens of type 1 are mainly involved in IgE antibodies, while type 4 is known to be an inflammatory response mainly through various lymphocytes, especially white blood cells, without the antibody. Atopic dermatitis is a disease caused by an allergic reaction and is characterized by hyperkeratosis, erythema, edema, severe pruritus, exudation and scab, as well as skin dryness. It is a disease accompanied by several symptoms such as dermatitis.

In the recent decades, the prevalence of atopic diseases has increased considerably in developed countries, and in Korea, the number of patients with atopic dermatitis is increasing with the proliferation of allergic diseases [YK Tay, KH Kong et al., J. Dermatology , 146, pp 101-106, 2002. In 1980, less than 1% of allergic patients, including atopic dermatitis, increased to more than 5% in the 2000s and more than 10% of potential patients. For this reason, due to the development of industry and civilization, the increase of environmental pollution, the change of diet, and the stress of stress have been mentioned [R. Barnetson, M. Rogers., Br. Medicine J. 324, pp1376-1379, 2002].

Atopic dermatitis is an immune disease caused by genetic and environmental factors. Atopic dermatitis is chronically dry, has a strong pruritus, recurs repeatedly, and dermatitis is easily induced by various stimuli. In many cases, the disease deteriorates repeatedly at intervals of 1 to 2 weeks, and if not properly managed, it progresses to subacute lesions of erythematous rashes with exudates and chronic lesions of thickened lichenitis, such as leather. In pathological (histochemical) findings of atopic dermatitis, the findings vary depending on the stage of the lesion. However, when chronic, the epidermis thickens and cells involve in various immune responses. In particular, spleen cells responsible for the immune response are increased and have an abnormally increased antigen presenting ability. In addition, the number of mast cells is increased by atopic dermatitis, indicating a degranulation state. Eighty to 90% of patients with atopic dermatitis have elevated serum IgE, especially if they are accompanied by respiratory atopy such as allergic rhinitis or asthma. In approximately 87% of patients with atopic dermatitis, skin tests or radioallergosorbent tests (RAST) test show positive IgE for various foods and inhaled allergens.If serum IgE is elevated, allergic diseases such as atopic dermatitis Is diagnosed as likely.

In oriental medicine, atopic dermatitis can be found in the categories of exten- sion, Taesun (태), Taechang (胎 瘡), and wet swelling (특히), especially in the case of infantile type (Kim Mi-jeong, Lee Seung-yeon. Korean Journal of Pediatric Science , 14, pp169-184, 2000; Park, Min-Chul et al., Korean Journal of Ophthalmology and Otolaryngology and Dermatology, 15, pp226-252, 2002; Baek, Jong-Hyun et al., The Korean Dermatological Society , 37, pp1553-1559, 1999]. Such causes include wind fever, blood fever, blood debris, rain, and gastrointestinal fever due to ataxia of malformation. Heat invasion and the like, in addition to stimulation such as changes in temperature, food, irregular feeding habits, clothing friction, changes in cold and cold [趙 純 修. Middle Dermatology . Science publisher. pp 110-112, 1999;顧伯華. Practical Surgery . Shanghai Science and Technology Press. pp 460-464, 1996.

In recent years, the research has been actively conducted due to the increase in allergic diseases as described above. Despite the advances in radical immunology, there have been many advances in the lifesaving and treatment of allergic diseases. There is no development of drugs that can cure. To date, atopic dermatitis has been treated with immunotherapy and pharmacotherapy, but there are no special treatments. In case of severe symptoms, steroids (adrenal corticosteroids) were used. Most of the effects are induced. Long-term topical administration of steroids leads to a decrease in adrenal function, resulting in side effects caused by various corticosteroids, which is inappropriate for continued use. As there is no appropriate treatment for patients with atopic dermatitis, in many cases relying on constitution improvement, folk remedies and oriental medical treatment, we expect to alleviate the clinical symptoms and cure, but there are no treatments that can be used without long-term side effects. It is true.

Recently, many studies for the treatment of allergic or atopic dermatitis using natural products have been made, and Korean Patent Publication No. 2004-97026 discloses a composition for preventing and treating atopic dermatitis using various natural products. 9172 also describes a constitution improvement composition of patients with atopic skin disease, and Korean Patent Laid-Open No. 2004-86790 discloses a treatment and prevention composition for allergic diseases using black rice extract, and Korea Patent Publication No. 2005-86 In 22586, atopic dermatitis treatment using a plantain extract is known. In addition, the Republic of Korea Patent Publication No. 2004-18118 discloses a composition for treating allergic diseases using the Darae extract which is one of the vegetable raw materials in the food raw material classification table of the Korea Food and Drug Administration, using the leaflet extract that can be used as food A composition for treating allergic diseases is also disclosed (Korean Patent Publication No. 2002-61963). Furthermore, Japanese Patent Application Laid-Open No. 2000-119127 describes that any one or more selected from lettuce extract, golden extract and salicylic acid can prevent or treat dermatitis. In addition, many pharmacological studies have been reported in relation to atopic dermatitis using natural products. Especially, it is known that Sopoong-san (영향 風 散) influences histamine release and lymphokine regulation in rats. Effect of Sopung Acid on Atopy Dermatitis Model using BALB / c Mouse. Kyung Hee University Graduate School, Ph.D. , 2002], Sangbaekpi is known to inhibit histamine release and degranulation of hypersensitivity shock and mast cells induced by Compound 48/80 [Kim, Jung-Su, Jeon, Byung-Deuk. Effect of Epithelium on Mesenteric Mast Cells in Rats. Jeonbuk Medical College , 10, pp231-239, 1986; Jeon Byung Deuk, Song Chang Ho, Young Sook Choi, Byeong Gun Park, and Moo Sam Lee. Effect of Epidermis on Sensitization Shock and Histamine Release of Mast Cells Induced by Compound 48/80. Journal of the Korean Society of Anatomy , 24, pp193-203, 1991].

Although many studies and patents using natural products have been published, there is no known drug, cosmetics, or foods that can treat or prevent allergic dermatitis using the herbal extract used in the present invention.

Accordingly, the present inventors selected herbal medicines that are effective in allergic skin diseases, and have a synergistic effect by prescribing a combination of extracts of Pung Pung or extracts of Pung Pung, Hojaja, ringworm, pastoral and white Jill to allergic skin diseases. The present invention has been completed by confirming that it is very effective.

Accordingly, it is an object of the present invention to provide a composition for preventing and treating allergic skin diseases, health supplements, and cosmetics, including the extract of saengpunggi or saengpung, yamja, ringworm skin, woodpecker and baekryeok complex.

The present invention is characterized by a composition for preventing and treating allergic skin diseases, including a saengpung extract or a saengpung, yamja, ringworm skin, woodpecker and baekryeok complex extracts, health supplements and cosmetics.

The composition comprising the herbal extract of the present invention, BALB / C mice and a 1-chloro-2 that induced atopic dermatitis by intraperitoneal injection of egg albumin (Avum albumin) and aluminum hydroxide (Aluminium hydroxide) and treated with phosphate buffer patch In the NC / Nga mice that induced atopic dermatitis by treatment with 4,4-dinitrobenzene, the number of scratches was remarkably suppressed, and in the sensory evaluation test, the atopic dermatitis score was also lowered. By showing the effect of reducing the elevation of IgE and IL4 can be useful as a composition for preventing and treating allergic skin diseases, dietary supplements and cosmetics.

Hereinafter, the present invention will be described in detail.

In the present invention, the number of scratches is greatly reduced in the animal model in which the saengpung extract or the saengpung, yamja, ringworm skin, woodpecker and white zirconia complex induced atopic dermatitis, and the sensory test has an effect of lowering the atopic dermatitis score. In addition, the present invention relates to a highly effective herbal extract and its use for allergic skin diseases by confirming the effect of reducing the elevation of IgE and IL4 in the blood according to the expression of atopic dermatitis.

First, looking at the herbal ingredients corresponding to each component of the composition according to the present invention.

Hydnocarpi semen is a seed of sapling tree or homologous root plant. It is a brownish-brown dull ridge of 2 ~ 3 cm in length and 1 ~ 2 cm in diameter, and the oily dark brown endosperm is removed until the hard seed is removed. The main components of this formula are Dungpoong Free, Hydnocarpin, and methoxyhydnocarpin, which contain hydnocarpic acid, chaulmoogric acid, and gorlic acid. And flavonoids such as flavognan and apigenin, luteolin, and chrysoeriol, such as hydnowightin and neoohydnocarpin.

Extraction of the macrophages with one or more solvents selected from water, methanol, ethanol and butanol to prepare the macrosporum extract, and the extract showed an excellent effect on allergic skin diseases such as atopy.

Khojaja is known as black sesame, nourishing tonic, viscous, detoxification, constipation, swelling effect is known.

Ringworm refers to the dermis of ringworm, and is known for its therapeutic efficacy, such as hepatic degeneration, headache, menstrual disorders, rheumatism, jaundice, and fever.

Momordicae Semen is a mature herbaceous, perennial vine, widely distributed in southern China, harvesting fruit from September to November, cutting it in two, cutting it in half, and removing the seeds or putting them in a jar to rot the skin. Separate the seeds when used. These pastors are known to have strong anti-inflammatory effects and are effective in rheumatoid pain and muscle spasms.

Tribulus terrestris L. is a Zygophyllaseae plant and its fruits are known to be effective in hypertension and stroke.

After mixing the saengpungja, Homaja, ringworm skin, woodpecker and white Zhijiri at the optimum mixing ratio, and extracted with one or more solvents selected from water, methanol, ethanol and butanol to prepare a herbal extract extract, the extract is atopy and The same effect was shown for allergic skin diseases. The optimum mixing ratio is the weight ratio of the saenggija, Hojaja, ringworm skin, woodpecker and white jilchi 1: 0.1 ~ 1.0: 0.01 ~ 0.5: 0.01 ~ 0.5: 0.01 ~ 0.5. More preferably, it is 1: 0.3-0.7: 0.05-0.3: 0.05-0.3: 0.05-0.3. If it is out of the above range, the desired effect is not expressed, and when used excessively, side effects may occur.

In addition, allergic skin such as atopic dermatitis may be extracted by extracting 0.01 to 0.5 parts by weight, preferably 0.05 to 0.3, based on 1 part by weight of saengpung ( Sophora flavcscens ) during the mixing of herbal medicines during the preparation of the complex extract (five species). Excellent effect on the disease. In addition, 0.01 to 0.5 parts by weight (more preferably 0.05 to 0.3 parts by weight) of Changgiza (dokkari), 1 part by weight of the saengpung in the process of mixing the herbal medicine during the preparation of the complex extract (including 6 kinds, goose ginseng), allium (burdock seed) 0.01 to 0.5 parts by weight (more preferably 0.05 to 0.3 parts by weight), man-shaped 0.01 to 0.5 parts by weight (more preferably 0.05 to 0.3 parts by weight), and gakguk 0.01 to 0.5 parts by weight Parts (more preferably 0.05 to 0.3 parts by weight), Baek Shou (0.01 to 0.5 parts by weight) (more preferably 0.05 to 0.3 parts by weight), mold opening (0.01 to 0.5 parts by weight) (more preferably 0.05 to 0.3 parts by weight), lieutenant line 0.01 to 0.5 parts by weight (more preferably 0.05 to 0.3 parts by weight), peppermint 0.01 to 0.5 parts by weight (more preferably 0.05 to 0.3 parts by weight) , 0.01-0.5 parts by weight (more preferably 0.05-0.3 parts by weight), 0.001-0.3 parts by weight (more preferably 0. 01 to 0.15 parts by weight), 0.01 to 0.5 parts by weight (more preferably 0.05 to 0.3 parts by weight), stoneworm 0.001 to 0.3 parts by weight (more preferably 0.01 to 0.15 parts by weight) and licorice (甘草) ) The efficacy was also confirmed by further extracting 0.01 to 0.5 parts by weight (more preferably, 0.05 to 0.3 parts by weight).

Meanwhile, the herbal extract of the present invention may be extracted after mixing the respective herbal medicines as described above, or may be mixed after obtaining the respective herbal medicine extracts.

In order to confirm the efficacy on the atopic dermatitis of the saengsugung extract or the herbal extract of the present invention intraperitoneally injected BALB / C mice with egg albumin and aluminum hydroxide and attach a phosphate buffer patch to induce atopic dermatitis, NC-Nga mice with predisposition to atopic dermatological diseases treated with 1-chloro-2,4-dinitrobenzene to induce atopic dermatitis, the frequency of scratching, sensory evaluation and expression of IgE and IL4 in the blood The amount was searched.

As a result, the herbal extracts were injected intraperitoneally with egg albumin and aluminum hydroxide and attached with phosphate buffer patch to significantly reduce the scratching frequency in proportion to the duration of administration in BALB / C mice that induced atopic dermatitis. In addition, the atopic dermatitis index, which indicates the degree of atopic dermatitis, was also decreased in proportion to the duration of administration, and the expression of IgE and IL4 in the blood was also significantly decreased. In addition, the frequency of the scratching frequency was significantly proportional to the administration period in the experimental animals that induced atopic dermatitis by treating 1 / chloro-2,4-dinitrobenzene in NC / Nga mice with atopic dermatitis predisposition. In addition, the atopic dermatitis index, which indicates the degree of atopic dermatitis, was also decreased in proportion to the duration of administration, and the expression of IgE and IL4 in the blood was also significantly decreased.

Accordingly, the present invention provides a composition for the prevention and treatment of allergic skin diseases comprising the saengpung extract or the herbal extract obtained above. The allergic skin disease is allergic dermatitis, atopic dermatitis, contact dermatitis, skin hypersensitivity, insect allergy, food allergy or drug allergy.

The composition for the prevention and treatment of allergic skin diseases of the present invention, it is preferable that the total saenggi extract or the herbal extracts in an amount of 0.02 to 50% by weight based on the total weight of the composition.

The composition comprising the extract of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

The pharmaceutical dosage forms of the extracts of the present invention may be used in the form of their pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable collection.

Compositions comprising the extracts according to the invention are oral formulations, such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations (ointments, patches, etc.), suppositories, and sterilization, respectively, according to conventional methods. It can be formulated and used in the form of injectable solutions. Examples of carriers, excipients and diluents that can be included in the composition containing the extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate , Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid form preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid form may include at least one excipient such as starch, calcium carbonate, sucrose in the extract. ) Or lactose, gelatin and the like are mixed. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups. In addition to commonly used simple diluents such as water and liquid paraffin, various excipients may be included, such as wetting agents, sweeteners, fragrances, and preservatives. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

In addition, the composition of the present invention may include a conventional substance which is known to be beneficial to the skin and the extract as an essential component. For example, it may include vitamin C, vitamin E, essential fatty acids and the like.

The preferred dosage of the composition of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art. However, for the desired effect, the composition of the present invention is preferably administered at 0.1 to 500 mg / kg, preferably 1 to 200 mg / kg. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

The composition of the present invention may be administered to mammals such as rats, mice, livestock, humans, and the like in various routes. All modes of administration can be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or Intracerebroventricular injection.

In addition, the present invention provides a dietary supplement comprising a composition for preventing and improving skin diseases, including a saengpung saeng extract or herbal extracts (5 species, 6 species or 19 species) obtained from the above, and a food supplement acceptable food additive. to provide.

Examples of the food to which the present composition can be added include various foods, gums, teas, vitamin complexes, health functional foods, powders, granules, tablets, capsules, and beverages.

It may also be added to foods or beverages for the purpose of treating and preventing allergic dermatitis. At this time, the amount of the composition in the food or beverage may be added in 0.01 to 15% by weight of the total food weight, the health beverage composition may be added in a ratio of 0.02 to 5 g, preferably 0.3 to 1 g based on 100 ml. have.

The health functional beverage composition of the present invention is not particularly limited to other ingredients except for containing the composition as an essential ingredient in the indicated ratios, and may contain various flavors or natural carbohydrates, etc. as additional ingredients, as in general beverages. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (tauumatin, stevia extract (e.g., Rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of said natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the dietary supplement of the present invention includes various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavoring agents, colorants and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid And salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the health supplements of the present invention may contain a flesh for preparing natural fruit juices and fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the total dietary supplement of the present invention.

In addition, the present invention provides a cosmetic for preventing and improving skin diseases, including the saengpung extract or herbal extracts (5, 6 or 19 species) obtained in the manufacturing process.

Examples of products that can be added to the cosmetics include cosmetics such as various creams, lotions, skins, and the like, and cleansing agents, face washes, soaps, treatments, and essences.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. The salts thereof (thiamine hydrochloride, sodium ascorbate salt, etc.) or derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) can also be used in the present invention. Included in The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (DL-alpha tocopherol, D-alpha tocopherol, d-alpha tocopherol), and the like. , Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, DL-alpha tocopherol acetate, DL-alpha tocopherol nicotinic acid, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl) Ethyl ether) and the like are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be incorporated into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics, but preferably, brown algae extract, red algae extract, green algae extract, and the like, and calginane, arginic acid, sodium arginate and argin purified from these seaweed extracts Potassium ginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to a total weight, More preferably, it is mix | blended in 0.01 to 3 weight%.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the composition as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

Cosmetic composition for preventing and improving skin diseases of the present invention can be prepared in any formulation commonly prepared in the art, for example, emulsion, cream, lotion, pack, foundation, lotion, essence, hair cosmetics, etc. Can be.

Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fibers, plant fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, and especially in the case of spray, additionally chlorofluorohydrocarbon, Propellant such as propane / butane or dimethylether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or sorbitan.

When the formulation of the present invention is a suspension, water, liquid diluents such as ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail based on Examples, but the present invention is not limited to the following Examples.

Preparation Example 1 Preparation of Saengpung Extract

240 g of air sac was finely chopped and extracted three times with ethanol three times each to obtain an ethanol extract, and the obtained extract was removed using a rotary vacuum concentrator to obtain ethanol extract.

Preparation Example 2 Preparation of Five Complex Herbal Extracts

Saeng 240g, Hog 120g, white ginseng skin 40g, woodpecker 40g and white ginseng 32g finely chopped, mixed and extracted three times with ethanol three times each to obtain an ethanol extract, the obtained extract is a rotary decompression concentrator After ethanol was removed using the ethanol extract for each herbal medicine.

Preparation Example 3 Preparation of Six Complex Herbal Extracts

40 g of wild ginseng was mixed in Preparation Example 2, and the same procedure as in Preparation Example 2 was performed to obtain six complex herbal extracts.

Preparation Example 4 Preparation of 19 Complex Herbal Extracts

Changxiza 40 g, wooja 32 g, Manza 32 g, Gamuk 32 g, Baekshao 40 g, hyunggae 40 g, Maeulseon 32 g, Mint 32 g, Cerebrum 32 g, Sulfur sulfur 20 g , 32 g of Seokchangpo, 12 g of worms and 32 g of licorice were added, and the same procedure as in Preparation Example 2 was carried out to obtain 19 complex herbal extracts.

Reference Example 1: Drugs and Reagents

Dexamethasone, aluminum hydroxide, ovum albumin, and chlorodinitrobenzene (DNCB) were purchased from Sigma-Aldrich Chemical Co. and others. Reagents were of the highest quality available commercially. All herbal medicines were purchased from Gyeongdong market (Seoul, Korea).

Reference Example 2: Preparation of Laboratory Animals

Four-week-old BALB / C mice (20-25 g) and NC / Ng mice (20-25 g) were supplied from a central laboratory animal (Seoul, Korea). It was used for 7 days, and water and feed were freely ingested, and the temperature (23 ± 2 ℃), humidity (55 ± 10%) and contrast cycle (12 hours) were automatically adjusted.

Reference Example 3: Statistics Processing

All experimental results were statistically analyzed using ANOVA (one way analysis of variance), and significance test was performed at p <0.05 level using Newman-Keuls Test as post-hoc test.

Experimental Example 1: Therapeutic Efficacy of Daengsugung Extract for Atopic Dermatitis

   After 4 weeks of age, the BALB / C mice were stabilized for 1 week, and then the atopy-induced group, the control group (TWEEN 80 10%), and the test substance-administered group (50 mg / kg, 150 mg / kg, 400 mg of Daegong extract of Preparation Example 1). mg / kg) and positive control group (Dexamethasone 5 mg / kg) were tested.

1) Induction of Atopic Dermatitis in BALB / C Mice

After the stabilization period, the mouse's back was removed from the bottom of the ear to the top of the tail, followed by intraperitoneal injection of 0.05 ml of 4% (w / w) aluminum hydroxide (Sigma) solution and 0.05 mg of egg albumin (OVA). 100 μl of phosphate buffer solution (PBS solution) was applied to the back and Tegadum (2 × 2 cm) was attached to prevent evaporation. The remaining groups were applied and attached with 100 μl of PBS solution and 0.1 mg OVA in the same manner. Three days later, the patch was removed and the incubation period was two weeks. Two weeks later, the control group was intraperitoneally injected with 50 μl of PBS solution for the second skin irritation, and the other groups were intraperitoneally injected with 50 μl of PBS solution and 50 μg OVA. Three days later, a second patch was attached to the back skin in the same way as the first patch. After 3 days, the attached patch was detached [Norikazu, 2003].

2) Administration of test drug

TWEEN 80 10% solution for the control animals, 50 mg / kg, 150 mg / kg, 400 mg / kg of the macrosatellite extract in the test animals, and 5 mg / of dexamethasone in the positive control animals. The dose of kg was orally administered once a day for 2 weeks between 10:00 AM and 11:00 AM. Daegongsae extract was administered suspended in a 10% solution of TWEEN 80.

3) Scratching behavior test

Test substance was administered at 1, 3, 7, 14 days after administration. Scratching counts were measured one hour before the test substance administration and 30 minutes after the test substance administration (Norikazu, 2003). Observations were made based on the number of behaviors that scrape the inflammatory site, a typical symptom of atopic dermatitis.

As shown in Figure 1 from the 3rd to 14th day treatment with the saengpungsaeng extract (A) group significantly reduced the number of scratches in the full dose group compared to the control group, the reduction effect was similar to the efficacy of dexamethasone. The above results suggest that administration of the saengpung extract for more than 3 days can alleviate the symptoms of atopic dermatitis.

4) sensory evaluation test

Test substance was administered at 1, 3, 7, 14 days after administration. This evaluation method is a clinical visual evaluation method commonly used in atopic dermatitis. The severity of atopic dermatitis is expressed as the sum of scores for each of the following five items. The endpoints were divided into erythema, itching and dry skin, edema and escoriation, erosion, and lichenification. Each item is scored as None (0), Weak (1), Moderate (2), and Severe (3) and summed to give a score between at least 0 and up to 15 points [Leung, et al., 1990 ; Suzuki, et al., 2002].

As a result of sensory evaluation, which is a combination of symptoms of atopic dermatitis, the atopic dermatitis scores of 150 mg / kg and 400 mg / kg of Saengpung extract were significantly lower than those of the control group, as shown in FIG. 2. At 14 days, atopic dermatitis scores were significantly decreased in the full dose group compared to the control group. The above results also suggest that administration of the saengpung extract for more than 7 days may alleviate the symptoms of atopic dermatitis.

5) Determination of plasma coral leukocytes (eosinophil) and IgE content

At 2 weeks after the administration of the test substance, the mouse was anaesthetized, cardiac blood was collected, and the blood leukocyte count was measured by a blood analyzer (Neodine Bevel Lab, Seoul), centrifuged (3,500 rpm, 7 minutes), and serum was separated. After storage at -80 ° C, the contents of coral leukocytes and Ig E were measured using an ELISA kit (Shibayagi. Co., Ltd. Japan) [Suzuki, et al, 2002; Kang. et al. 2006]. An increase in coral leukocytes and IgE, a representative hematological change in the expression of atopic dermatitis, was observed. As shown in FIG. 3, the number of coral leukocytes in the full dose group was significantly decreased compared to the control group, and the plasma IgE content was significantly decreased in the control group in the 150 mg / kg and 400 mg / kg administration groups. The results also suggest that the saengpung extract can alleviate the symptoms of atopic dermatitis, and the alleviation of the symptoms is related to allergic reactions.

Experimental Example 2: Therapeutic Efficacy of Herbal Combination Extract (5 Types) against Atopic Dermatitis

BALB / C mice at 4 weeks of age were stabilized for 1 week, after which atopic dermatitis-induced group, control group (TWEEN 80 10%), test substance-administered group (5 herbal compound combination extract of Preparation Example 2 40 mg / kg, 200 mg / kg, 500 mg / kg) and positive control (Dexamethasone 5 mg / kg) was divided into tests.

1) Induction of Atopic Dermatitis in BALB / C Mice

After the stabilization period, the mouse's back was removed from the bottom of the ear to the top of the tail, followed by intraperitoneal injection of 0.05 ml of 4% (w / w) aluminum hydroxide (Sigma) solution and 0.05 mg of egg albumin (OVA). 100 μl of phosphate buffer solution (PBS solution) was applied to the back and Tegadum (2 × 2 cm) was attached to prevent evaporation. The remaining groups were applied and attached with 100 μl of PBS solution and 0.1 mg OVA in the same manner. Three days later, the patch was removed and the incubation period was two weeks. Two weeks later, the control group was intraperitoneally injected with 50 μl of PBS solution for the second skin irritation, and the other groups were intraperitoneally injected with 50 μl of PBS solution and 50 μg OVA. Three days later, a second patch was attached to the back skin in the same way as the first patch. After 3 days the attached patch was removed.

2) Administration of test drug

TWEEN 80 10% solution for control animals, 5 compound herbal extracts of 40 mg / kg, 200 mg / kg, 500 mg / kg for test animals, and dexamethasone for positive control animals. The dose of 5 mg / kg was orally administered once a day for 2 weeks between 10:00 AM and 11:00 AM.

3) Scratching Behavior Test

Test substance was administered at 1, 3, 7, 14 days after administration. Scratching counts were measured one hour before the test substance administration and 30 minutes after the test substance administration.

Observations were made based on the number of behaviors that scrape the inflammatory site, a typical symptom of atopic dermatitis. By the 14th day of the treatment, the four herbal compound extracts were significantly reduced the number of scratches in the full dose group compared to the control group, and the reduction effect was similar to or stronger than that of dexamethasone [FIG. 4]. The tendency to decrease after treatment was stronger than before treatment. The above results could alleviate the symptoms of atopic dermatitis by administering five herbal extracts for more than three days.

4) sensory evaluation test

Test substance was administered at 1, 3, 7, 14 days after administration. This evaluation method is a clinical visual evaluation method commonly used in atopic dermatitis. The severity of atopic dermatitis is expressed as the sum of scores for each of the following five items. The endpoints were divided into erythema, itching and dry skin, edema and escoriation, erosion, and lichenification. Each item is scored as None (0), Weak (1), Moderate (2), and Severe (3) and summed to give a score between 0 and 15 points.

According to the results of sensory evaluation by combining the symptoms of atopic dermatitis, the atopic dermatitis scores of 200 mg / kg and 500 mg / kg combinations of five herbal extracts were significantly lower than those of the control group on the 3rd and 7th day of treatment. On the 14th day, the atopic dermatitis score was significantly decreased in the full dose group compared to the control group [FIG. 5]. The decreasing efficacy was similar or stronger than that of dexamethasone. The above results also suggest that administration of five herbal extracts for more than 3 days may alleviate the symptoms of atopic dermatitis.

5) Measurement of Plasma IgE and IL-4 Levels

Two weeks after the test substance was administered, mice were anesthetized, heart blood was collected, centrifuged (3,500 rpm, 7 minutes), serum was separated, stored at -80 ° C, and then ELISA kit (Shibayagi. Co., Ltd. Japan) was used to measure the content of IgE and IL-4.

IL4 and IgE, which are representative hematological changes in the expression of atopic dermatitis, were observed as indicators. Changes in plasma levels of IgE and IL-4 exemplify the extent of inflammatory and immune responses. In particular, IgE is an indicator of the extent of allergic reactions. In the case of IL4, blood concentration was increased by the induction of atopic dermatitis, but was significantly recovered by the administration of 200 mg / kg of five complex herbal extracts and 5 mg / kg of dexamethasone. IgE also increased blood levels due to the induction of atopic dermatitis, but was significantly recovered by administration of five complex herbal extracts 40, 200, 500 mg / kg and dexamethasone 5 mg / kg [FIG. 6]. The efficacy of the five herbal extracts on the changes of IgE and IL-4 in the blood was also similar or stronger than that of dexamethasone. The above results also suggest that the five herbal extracts can alleviate the symptoms of atopic dermatitis, and the alleviation of the symptoms is associated with allergic reaction inhibitory action.

Experimental Example 3: Therapeutic Efficacy of Herbal Combination Extract (6 Species) against Atopic Dermatitis

NC / Nga mice at 4 weeks of age were stabilized for 1 week, after which atopic dermatitis-induced group, control group (TWEEN 80 10%), test substance-administered group (6 herbal compound complex extracts of Preparation Example 3 40 mg / kg, 200 mg / kg, 500 mg / kg) and positive control (Dexamethasone 5 mg / kg) was divided into tests.

1) Atopic Induction Test

After the stabilization period, the mouse's back is epilated from the bottom of the ear to the top of the tail and left for 24 hours, followed by 200 μl of 1% DNCB solution (acetone: olive oil = 3: 1) to induce immunization. It was applied to the site. After 4 days of application, 150 μl of 0.2% DNCB solution was re-applied three times a week for 5 weeks to induce atopic dermatitis.

2) Administration of test drug

After the DNCB treatment, the control animals were treated with a 10% solution of TWEEN 80, and 6 animals of the herbal extracts of the test group were dosed at 40 mg / kg, 200 mg / kg, and 500 mg / kg. Animals were orally administered dexamethasone (Dexamethasone) at a dose of 5 mg / kg once a day between 10:00 AM and 11:00 AM.

3) Scratching Behavior Test

Test substance was administered at 1, 3, 7 days after administration. Scratching counts were measured one hour before the test substance administration and 30 minutes after the test substance administration.

 Observations were made based on the number of behaviors that scrape the inflammatory site, a typical symptom of atopic dermatitis. The number of scratches was significantly decreased in the full dose group compared to the control group after the six combination herbal extracts were administered on the first day of the treatment, and the number of scratches was significantly reduced in the 200 mg / kg and 500 mg / kg groups compared with the control group. Was similar or stronger than the efficacy of dexamethasone [FIG. 7]. The above results suggest that administration of six herbal extracts can alleviate the symptoms of atopic dermatitis.

4) sensory evaluation test

Test substance was administered at 1, 3, 7 days after administration. This evaluation method is a clinical visual evaluation method commonly used in atopic dermatitis. The severity of atopic dermatitis is expressed as the sum of scores for each of the following five items. The endpoints were divided into erythema, itching and dry skin, edema and escoriation, erosion, and lichenification. Each item is scored as None (0), Weak (1), Moderate (2), and Severe (3) and summed to give a score between 0 and 15 points.

According to the results of sensory evaluation by combining the symptoms of atopic dermatitis, the atopic dermatitis scores of 200 mg / kg and 500 mg / kg of the six combination herbal extracts were significantly decreased in the 3rd and 7th day of treatment. [FIG. 8]. The decreasing efficacy was similar or stronger than that of dexamethasone. The above results also suggest that administration of the six herbal extracts can alleviate the symptoms of atopic dermatitis.

5) Measurement of Plasma IgE and IL-4 Levels

After 1 week of administration of the test substance, mice were anesthetized, heart blood was collected, centrifuged (3,500 rpm, 7 minutes), serum was separated, stored at -80 ° C, and the ELISA kit (Shibayagi. Co., Ltd. Japan) was used to measure the content of IgE and IL-4.

IL4 and IgE, which are representative hematological changes in the expression of atopic dermatitis, were observed as indicators. Changes in plasma levels of IgE and IL-4 foreshadow the extent of inflammatory and immune responses, and in particular, IgE is an indicator of the extent of allergic reactions. In the case of IL4, the blood concentration was increased by the induction of atopic dermatitis, but it was significantly recovered by the administration of 200 mg / kg of 6 herbal extracts and 5 mg / kg of dexamethasone. IgE also increased blood levels due to the induction of atopic dermatitis, but recovered significantly by administration of 500 mg / kg of the six complex herbal extracts [FIG. 9]. The efficacy of the six herbal extracts on the changes of IgE and IL-4 levels in the blood was similar or stronger than that of dexamethasone. The above results also suggest that the six herbal extracts can alleviate the symptoms of atopic dermatitis, and the alleviation of the symptoms is related to allergic reaction inhibitory action.

Test Example 4: Therapeutic Efficacy of Herbal Combination Extract (19 Types) against Atopic Dermatitis

BALB / C mice at 4 weeks of age were stabilized for 1 week, after which atopic non-induced group, control group (TWEEN 80 10%), test substance-administered group (complex extract of 19 kinds of herbal medicines of Preparation Example 4 100 mg / kg, 500 mg / kg) The test was performed by classifying into a positive control group (Dexamethasone 5 mg / kg).

1) Induction of Atopic Dermatitis in BALB / C Mice

After the stabilization period, the mouse's back was removed from the bottom of the ear to the top of the tail, followed by intraperitoneal injection of 0.05 ml of 4% (w / w) aluminum hydroxide (Sigma) solution and 0.05 mg of egg albumin (OVA). 100 μl of phosphate buffer solution (PBS solution) was applied to the back and Tegadum (2 × 2 cm) was attached to prevent evaporation. The remaining groups were applied and attached with 100 μl of PBS solution and 0.1 mg OVA in the same manner. Three days later, the patch was removed and the incubation period was two weeks. Two weeks later, the control group was intraperitoneally injected with 50 μl of PBS solution for the second skin irritation, and the other groups were intraperitoneally injected with 50 μl of PBS solution and 50 μg OVA. Three days later, a second patch was attached to the back skin in the same way as the first patch. After 3 days the attached patch was removed.

2) Administration of test drug

TWEEN 80 10% solution was used in the control animals, and 19 mg of the herbal extracts in the test group were dosed at 100 mg / kg and 400 mg / kg, and dexamethasone was 5 mg / kg in the positive control animals. The dose was administered orally once a day for two weeks between 10:00 AM and 11:00 AM.

3) Scratching Behavior Test

Test substance was administered at 1, 3, 7, 14 days after administration. Scratching counts were measured one hour before the test substance administration and 30 minutes after the test substance administration.

By the 14th day of treatment, the group of 19 herbal compound extracts was significantly reduced in the number of scratches in the full dose group compared to the control group, and the reduction effect was similar to or stronger than that of dexamethasone [FIG. 10]. The above results could alleviate the symptoms of atopic dermatitis when administered over 19 days with 19 herbal extracts.

4) sensory evaluation test

Test substance was administered at 1, 3, 7, 14 days after administration. This evaluation method is a clinical visual evaluation method commonly used in atopic dermatitis. The severity of atopic dermatitis is expressed as the sum of scores for each of the following five items. The assessment items are divided into erythema, itching and dry skin, edema and edema, edema and erosion, and lichenification. Each item is scored as None (0), Weak (1), Moderate (2), and Severe (3) and summed to give a score between 0 and 15 points.

As a result of sensory evaluation by combining the symptoms of atopic dermatitis, atopic dermatitis scores were significantly reduced in the group of 19 herbal compound extracts only on the 7th and 14th day of drug treatment compared to the control group [FIG. 11]. The decreasing efficacy was similar or stronger than that of dexamethasone. The above results also suggest that administration of the 19 herbal extracts for more than 3 days may alleviate the symptoms of atopic dermatitis.

Test Example 4: Oral Administration of Rats Acute Toxicity

Acute toxicity test was performed using 6-week-old SPF SD rats as follows.

Two animals per group were dissolved in the macrosatellite extract or the herbal extract, respectively, and administered once orally at a dose of 1 g / kg. After administration of the test substance, mortality, clinical symptoms, and changes in body weight were observed. Hematological and hematological examinations were performed. Necropsy was performed to observe abdominal and thoracic organ abnormalities.

As a result, no significant clinical symptoms or dead animals were noted in all animals treated with the test substance, and no toxic changes were observed in weight changes, blood tests, blood biochemical tests, and autopsy findings. Therefore, saengsugung extract or herbal extract according to the present invention did not show a toxicity change to 2000 mg / kg in all rats.

Formulation Example 1 Preparation of Powder

Saenggi extract or herbal extract 300 mg

Lactose 100 mg

Talc 10 mg

The above ingredients were mixed and filled in an airtight cloth to prepare a powder.

Formulation Example 2: Preparation of Tablet

Saenggi extract or herbal extract 300 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components was prepared by tableting according to the conventional manufacturing method of the tablet.

Formulation Example 3: Preparation of Capsule

Saenggi extract or herbal extract 300 mg

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium Stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients were mixed and filled into gelatin capsules to prepare capsules.

Formulation Example 4 Preparation of Injection

Saenggi extract or herbal extract 300 mg

Mannitol 180 mg

Sterile distilled water for injection 2974 mg

_{Na 2 HPO 4 · 12H 2 O} 26 mg

According to the conventional method for preparing an injection, the amount of the above ingredient was prepared per ampoule (2 ml).

Formulation Example 5 Preparation of Liquid

Saenggi extract or herbal extract 300 mg

10 g of isomerized sugar

5 g of mannitol

Purified water

According to the conventional method of preparing a liquid solution, each component is added to the purified water to dissolve, the lemon flavor is added appropriately, the above components are mixed, purified water is added, the whole is adjusted to 100 ml by the addition of purified water, and then filled into a brown bottle. The solution was prepared by sterilization.

Formulation Example 6: Preparation of Ointment

Using the 80 parts by weight of the extract of saengpung or herbal extracts ointment was prepared in the following composition.

[Furtherance]

Active ingredient 5 g, cetyl palmitate 20 g, cetanol 40 g, stearyl alcohol 40 g, myristan isopropyl 80 g, monostearic acid sorbitan 20 g, polysorbate 60 g, paraoxybenzoic acid propyl 1 g, 1 g of methyl paraoxybenzoate, phosphoric acid and purified water

Formulation Example 7 Preparation of Dietary Supplement

Daegong Extract or Herbal Extract 1000 mg

Vitamin mixture proper amount

70 μg of Vitamin A Acetate

Vitamin E 1.0 mg

0.13 mg of vitamin B ₁

0.15 mg of vitamin B ₂

0.5 mg of vitamin B ₆

Vitamin B ₁₂ 0.2 g

Vitamin C 10 mg

10 μg biotin

Nicotinic Acid 1.7 mg

50 μg folic acid

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

Formulation Example 8: Preparation of Healthy Drinks

Daegong Extract or Herbal Extract 1000 mg

Citric acid 1000 mg

100 g oligosaccharides

Plum concentrate 2 g

1 g of taurine

Add 900 ml of purified water

After mixing the above components according to the conventional healthy beverage production method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed, sterilized and refrigerated Next was used to prepare a health beverage composition of the present invention. Although the compositional ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the compounding ratio according to the regional or national preference such as the demand class, the demanding country, and the use purpose.

Formulation Example 9 Preparation of Bath Liquid

A bath solution may be prepared by mixing 21 parts by weight of DL-sodium lactate, 8 parts by weight of sodium pyruvate, 45 parts by weight of saucy extract or herbal extract, 26 parts by weight of purified water, and an appropriate amount of pigment and flavor. It is suitable for use as a bath for improving skin diseases by diluting 100 to 10,000 times in bath water when used. In this case, a cleansing liquid, astringent liquid and a moisturizing liquid can be used instead of the bath water.

Formulation Example 10 Preparation of Skin Lotion

3 parts by weight of glycerin, 2 parts by weight of butylene glycol, 2 parts by weight of propylene glycol, and preservatives were added and dissolved in purified water, and then 1 part by weight of polyoxyethylene (60) cured castor oil was dissolved by heating to 60 ° C. Was added and stirred to add 80.9 parts by weight of purified water. Finally, 1 part by weight of the macrosporum extract or the herbal extract extract, 10 parts by weight of ethanol, 0.1 parts by weight of triethanol amine, and a pigment were added thereto, followed by sufficiently stirring to obtain the title product. This product is used as a high quality skin beauty and skin disease improvement agent.

Formulation Example 11 Preparation of Milk Lotion (Nutrition Lotion)

1.7 parts by weight of cytosterol, 1.5 parts by weight of polyglyceryl 2-oleate, 0.7 parts by weight of ceramide, 1.2 parts by weight of ceteareth-4, and 1.5 parts by weight of cholesterol are homogenized at a constant temperature, 1 part by weight of saengsugung extract or herbal extract 0.4 parts by weight of dicetyl phosphate, 5.0 parts by weight of concentrated glycerin, 10 parts by weight of sunflower oil, 0.2 parts by weight of carboxyvinyl polymer, 0.3 parts by weight of xanthan gum, and a preservative by heating and dissolving it in a conventional manner, and then adding 76.5 parts by weight of purified water to the solution. After emulsifying in the air, an appropriate amount of perfume was added and stirred and mixed again to obtain the title product. This product is used as a high quality skin care agent and skin disease improver.

1 is a result showing the effect of the macrosporum extract on the scratching frequency of the experimental animals in BALB / C mice induced with atopic dermatitis by intraperitoneal injection of egg albumin and aluminum hydroxide and attached to a phosphate buffer patch [ A: Satun Extract],

FIG. 2 shows the results of observation of the effect of the macrosporum extract on sensory evaluation in BALB / C mice induced with atopic dermatitis by intraperitoneal injection of egg albumin and aluminum hydroxide and attachment of a phosphate buffer patch [A: macrosporus extract],

Fig. 3 shows the results of the macrophages extracts on the changes of eosinophil and Ig E contents in BALB / C mice induced with atopic dermatitis by intraperitoneal injection of egg albumin and aluminum hydroxide and attachment of phosphate buffer patches. [A: Satun Extract].

4 is a combination of five herbal extracts (UC1) for the scratching frequency of experimental animals in BALB / C mice induced with atopic dermatitis by intraperitoneal injection of egg albumin and aluminum hydroxide and attachment of a phosphate buffer patch. Was observed for 60 minutes [A: 1, B: 3, C: 7, D: 14],

FIG. 5 shows the results of observing the effects of five herbal extracts (UC1) on sensory evaluation in BALB / C mice induced with atopic dermatitis by intraperitoneal injection of egg albumin and aluminum hydroxide and attachment of a phosphate buffer patch. [A: 1, B: 3, C: 7, D: 14],

FIG. 6 shows the effects of five herbal extracts (UC1) on IgE and IL4 in BALB / C mice induced with atopic dermatitis by intraperitoneal injection of egg albumin and aluminum hydroxide and attachment of phosphate buffer patches. ego,

7 is a combination of six herbal extracts (UC1) for the scratching frequency of experimental animals in NC / Nga mice induced with atopic dermatitis by treatment with DNCB (1-chloro-2,4-dinitrobenzene) The effect was observed for 60 minutes [A: 1, B: 3, C: 7],

FIG. 8 shows the effects of six herbal extracts (UC1) for 60 minutes on sensory evaluation in NC / Nga mice that induced atopic dermatitis by DNCB [A: 1, B: 3, C: 7],

FIG. 9 shows the results of 60 minutes of the effects of six herbal extracts (UC1) on IgE and IL4 in NC / Nga mice that induce atopic dermatitis by DNCB treatment.

Figure 10 is a combination of 19 herbal extracts (C) for the scratching frequency of experimental animals in BALB / C mice in which intraperitoneal injection of egg albumin and aluminum hydroxide and phosphate buffer patch induced atopic dermatitis [A: 1, B: 3, C: 7, D: 14],

FIG. 11 shows the effect of 19 herbal extracts (C) on sensory evaluation in BALB / C mice induced with atopic dermatitis by intraperitoneal injection of egg albumin and aluminum hydroxide and attachment of a phosphate buffer patch [A : 1, B: 3, C: 7, D: 14].

Claims (16)

A pharmaceutical composition for the prevention and treatment of allergic dermatitis or atopic dermatitis, which comprises the macrosparagus extract extracted from the macrospores with one or more solvents selected from water, methanol, ethanol and butanol.  A pharmaceutical composition for the prevention and treatment of allergic dermatitis or atopic dermatitis, comprising a complex extract obtained by extracting macrophages, horseshoe, ringworm, shepherds, and whites with one or more solvents selected from water, methanol, ethanol and butanol. The method of claim 2, wherein the complex extract is characterized in that the saengpungja, Homaja, ringworm skin, woodpecker and Baekjichiri 1: 1 ~ 0.1 ~ 1.0: 0.01 ~ 0.5: 0.01 ~ 0.5: 0.01 ~ 0.5 Pharmaceutical composition. The pharmaceutical composition according to claim 3, further comprising 0.01 to 0.5 parts by weight of ginseng per 1 part by weight of saengsung during the mixing of the herbal medicines. The method according to claim 4, wherein when the herbal medicine is mixed, Chang-za is 0.01 to 0.5 parts by weight, allium 0.01 to 0.5 parts by weight, man-shaped 0.01 to 0.5 parts by weight, 0.01 to 0.5 parts by weight, and 100 to 100 parts by weight. ~ 0.5 parts by weight, mold opening 0.01 ~ 0.5 parts by weight, lieutenant line 0.01 ~ 0.5 parts by weight, peppermint 0.01 ~ 0.5 parts by weight, cinnabar 0.01 ~ 0.5 parts by weight, sulfur sulfur 0.001 ~ 0.3 parts by weight, stone spear 0.01 ~ 0.5 parts by weight, total shock 0.001 A pharmaceutical composition comprising ~ 0.3 parts by weight and licorice 0.01 ~ 0.5 parts by weight. delete delete The pharmaceutical composition according to any one of claims 1 to 5, wherein the pharmaceutical composition is a tablet, a pill, a powder, a capsule, a liquid, a suspension, a granule, a syrup, an injection, or an external preparation. A dietary supplement for the prevention and improvement of allergic dermatitis or atopic dermatitis, which comprises the macrosparagus extract extracted from the macrospores with one or more solvents selected from water, methanol, ethanol and butanol. A health supplement for the prevention and improvement of allergic dermatitis or atopic dermatitis, which comprises a complex extract obtained by extracting the macrophages, horseshoe, ringworm skin, woodpecker, and baekryegi with at least one solvent selected from water, methanol, ethanol and butanol. The dietary supplement of claim 10, wherein the extract further contains red ginseng. 12. The health supplement according to claim 11, wherein the extract further contains Chang-za, ally, man-shaped, gamguk, baekhsao, hyungae, lieutenant, peppermint, yongnae, sulphur, changchangpo, jeonchung and licorice. A cosmetic for preventing and improving allergic dermatitis or atopic dermatitis, which comprises the macrosparagus extracted with macrophages in at least one solvent selected from water, methanol, ethanol and butanol. A cosmetic for preventing and improving allergic dermatitis or atopic dermatitis, comprising a complex extract obtained by extracting macrophages, horseshoe, ringworm skin, shepherds, and whites with one or more solvents selected from water, methanol, ethanol and butanol. The cosmetic according to claim 14, wherein the extract further contains red ginseng. [Claim 16] The cosmetic of claim 15, wherein the extract further contains Chang-za, ally, man-shaped, gamguk, Baek-shou-opened gyeonghyeong, lieutenant gland, peppermint, dragon's head, quail sulphate, stone spear, worm, and licorice.

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