JP7455350B2 - Damage inhibitors, cosmetics, and food and beverages caused by air pollutants - Google Patents
Damage inhibitors, cosmetics, and food and beverages caused by air pollutants Download PDFInfo
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- JP7455350B2 JP7455350B2 JP2019190185A JP2019190185A JP7455350B2 JP 7455350 B2 JP7455350 B2 JP 7455350B2 JP 2019190185 A JP2019190185 A JP 2019190185A JP 2019190185 A JP2019190185 A JP 2019190185A JP 7455350 B2 JP7455350 B2 JP 7455350B2
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Description
本発明は、環境汚染物質によるダメージ抑制剤に関するものである。さらに、本発明は、環境汚染物質によるダメージ抑制用途に用いられる化粧料及び飲食品にも関する。 The present invention relates to an agent for suppressing damage caused by environmental pollutants. Furthermore, the present invention also relates to cosmetics and food/beverage products used to suppress damage caused by environmental pollutants.
近年、工業化による大気汚染や水質汚染等の環境問題に対して関心が集まっている。工場からの排水、排煙及び自動車の排気ガス等には様々な環境汚染物質が含まれており、硫黄酸化物及び窒素酸化物等のガス状汚染物質からＰＭ２．５等の粒子状物質まで様々なものがある。 In recent years, there has been growing interest in environmental problems such as air pollution and water pollution caused by industrialization. Wastewater from factories, flue gas, and automobile exhaust gas contain various environmental pollutants, ranging from gaseous pollutants such as sulfur oxides and nitrogen oxides to particulate matter such as PM2.5. There is something.
環境汚染物質は、吸引したり皮膚に付着したりすることにより、気管支炎、喘息、発癌、心疾患、骨や軟骨の破壊、発熱、皮膚の保水力低下、皮膚のバリア機能低下、肌荒れ及び炎症等を引き起こす。中でも環境汚染物質の一つである多環式芳香族炭化水素は、窒素化合物と反応することで変異（癌）原物質を含む二次生成物質を生成することが知られている。このように、環境汚染物質による人体へのダメージは重要な問題となっている。 When inhaled or attached to the skin, environmental pollutants can cause bronchitis, asthma, cancer, heart disease, destruction of bones and cartilage, fever, decreased skin water retention, decreased skin barrier function, rough skin, and inflammation. etc. Among them, polycyclic aromatic hydrocarbons, which are one of the environmental pollutants, are known to generate secondary products including mutagenic (cancer) substances by reacting with nitrogen compounds. As described above, damage to the human body caused by environmental pollutants has become an important problem.
従来では、大気汚染物質（重金属）からの皮膚、頭皮、毛髪及び外部粘膜の保護における、オーク抽出物、ブドウ種子抽出物及び緑茶抽出物を含む化粧品組成物が知られている（特許文献１）。 Cosmetic compositions containing oak extract, grape seed extract and green tea extract in the protection of the skin, scalp, hair and external mucous membranes from air pollutants (heavy metals) are known (Patent Document 1) .
本発明は、安全性の高い天然物由来成分の中から、環境汚染物質によるダメージ抑制作用を有するものを見出し、それを有効成分とする環境汚染物質によるダメージ抑制剤並びに当該天然物由来成分を配合した化粧料及び飲食品を提供することを目的とする。 The present invention has discovered an ingredient that has the effect of inhibiting damage caused by environmental pollutants from among ingredients derived from highly safe natural products, and has formulated an agent for inhibiting damage caused by environmental pollutants containing this ingredient as an active ingredient, as well as the ingredient derived from the natural product. The purpose is to provide cosmetics, food and beverages with
本発明者らは上記問題を解決すべく研究を行った結果、スターフルーツ（Averrhoa carambola L.）の果実からの抽出物が、環境汚染物質によるダメージ抑制作用において優れた作用効果を有することを見出し、本発明を完成させた。具体的には、本発明は以下のとおりである。 The present inventors conducted research to solve the above problem and found that an extract from the fruit of star fruit (Averrhoa carambola L.) has an excellent action and effect in suppressing damage caused by environmental pollutants. , completed the present invention. Specifically, the present invention is as follows.
〔１〕スターフルーツ（Averrhoa carambola L.）の果実からの抽出物を有効成分とすることを特徴とする、環境汚染物質によるダメージ抑制剤。
〔２〕環境汚染物質によるタンパク質カルボニル化を抑制する用途及び／又は環境汚染物質による炎症関連遺伝子の発現上昇を抑制する用途に用いられる、請求項１に記載の環境汚染物質によるダメージ抑制剤。
〔３〕スターフルーツ（Averrhoa carambola L.）の果実からの抽出物を配合したことを特徴とする、環境汚染物質によるダメージ抑制用化粧料。
〔４〕スターフルーツ（Averrhoa carambola L.）の果実からの抽出物を配合したことを特徴とする、環境汚染物質によるダメージ抑制用飲食品。
[1] An agent for suppressing damage caused by environmental pollutants, characterized by containing an extract from the fruit of star fruit (Averrhoa carambola L.) as an active ingredient.
[2] The agent for suppressing damage caused by environmental pollutants according to claim 1, which is used for suppressing protein carbonylation caused by environmental pollutants and/or for suppressing increased expression of inflammation-related genes caused by environmental pollutants.
[3] A cosmetic for suppressing damage caused by environmental pollutants, which is characterized by containing an extract from star fruit (Averrhoa carambola L.).
[4] A food/beverage product for suppressing damage caused by environmental pollutants, which is characterized by containing an extract from the fruit of star fruit (Averrhoa carambola L.).
本発明によれば、スターフルーツ（Averrhoa carambola L.）の果実からの抽出物を有効成分とすることにより、作用効果に優れかつ安全性の高い、環境汚染物質によるダメージ抑制剤を提供することができる。さらに、スターフルーツ（Averrhoa carambola L.）の果実からの抽出物を配合することで、環境汚染物質によるダメージ抑制用途に好適な化粧料及び飲食品を提供することができる。 According to the present invention, by using an extract from star fruit (Averrhoa carambola L.) as an active ingredient, it is possible to provide a highly effective and highly safe agent for suppressing damage caused by environmental pollutants. can. Furthermore, by incorporating an extract from star fruit (Averrhoa carambola L.), cosmetics, food and drink products suitable for use in suppressing damage caused by environmental pollutants can be provided.
以下、本発明の実施の形態について説明する。
本実施形態の環境汚染物質によるダメージ抑制剤は、スターフルーツの果実からの抽出物を有効成分とするものである。また、本実施形態の化粧料及び飲食品は、環境汚染物質によるダメージ抑制用途に用いられるものであって、スターフルーツの果実からの抽出物が配合されるものである。
Embodiments of the present invention will be described below.
The agent for suppressing damage caused by environmental pollutants of the present embodiment contains an extract from star fruit as an active ingredient. Furthermore, the cosmetics and food/beverage products of the present embodiment are used to suppress damage caused by environmental pollutants, and contain an extract from star fruit.
ここで、本実施形態における「スターフルーツの果実からの抽出物」には、抽出処理によってスターフルーツの果実から得られる抽出液、該抽出液の希釈液若しくは濃縮液、該抽出液を乾燥して得られる乾燥物、又はこれらの粗精製物若しくは精製物のいずれもが含まれる。 Here, the "extract from star fruit fruit" in this embodiment includes an extract obtained from star fruit fruit by an extraction process, a diluted or concentrated solution of the extract, and a product obtained by drying the extract. This includes the obtained dried product, or either a crudely purified product or a purified product thereof.
本実施形態において使用する抽出原料は、スターフルーツ（学名：Averrhoa carambola L.）の果実である。スターフルーツはカタバミ科に属し、五斂子、陽桃（生薬名）とも呼ばれ、その果実は食用されている。スターフルーツは、中国では紀元前から文献に記載され、その果実は断面が星形のことからスターフルーツと呼ばれている。スターフルーツは沖縄、中国東南部や雲南その他熱帯各地で栽培されており、これらの地域から容易に入手することができる。 The extraction raw material used in this embodiment is the fruit of star fruit (scientific name: Averrhoa carambola L.). Star fruit belongs to the Oxalis family, and is also called five star fruit or yang peach (herbal medicine name), and its fruit is edible. Starfruit has been described in Chinese literature since B.C., and its fruit is called starfruit because of its star-shaped cross section. Starfruit is cultivated in Okinawa, southeastern China, Yunnan, and other tropical regions, and can be easily obtained from these regions.
抽出原料として使用するスターフルーツの果実は、採取後ただちに乾燥したものが適当である。乾燥は天日で行ってもよいし、通常使用される乾燥器を用いて行ってもよい。スターフルーツの果実は乾燥後、粉砕や破砕等の処理を行ってもよい。スターフルーツの果実は、ヘキサン等の非極性溶媒によって脱脂等の前処理を施してから抽出原料として使用してもよい。脱脂等の前処理を行うことにより、スターフルーツの果実の極性溶媒による抽出処理を効率よく行うことができる。 The star fruit used as the raw material for extraction is suitably dried immediately after being collected. Drying may be performed in the sun or using a commonly used dryer. After drying, the star fruit fruit may be subjected to treatments such as crushing or crushing. The fruit of star fruit may be used as an extraction raw material after being subjected to pretreatment such as defatting with a nonpolar solvent such as hexane. By performing pre-treatment such as defatting, star fruit can be efficiently extracted with a polar solvent.
抽出溶媒としては、極性溶媒を使用することが好ましく、例えば、水、親水性有機溶媒等が挙げられ、これらを単独で又は２種以上を組み合わせて、室温又は溶媒の沸点以下の温度で使用することが好ましい。 As the extraction solvent, it is preferable to use a polar solvent, such as water, hydrophilic organic solvents, etc., which are used alone or in combination of two or more at room temperature or at a temperature below the boiling point of the solvent. It is preferable.
抽出溶媒として使用し得る水としては、純水、水道水、井戸水、鉱泉水、鉱水、温泉水、湧水、淡水等のほか、これらに各種処理を施したものが含まれる。水に施す処理としては、例えば、精製、加熱、殺菌、濾過、イオン交換、浸透圧調整、緩衝化等が含まれる。したがって、本実施形態において抽出溶媒として使用し得る水には、精製水、熱水、イオン交換水、生理食塩水、リン酸緩衝液、リン酸緩衝生理食塩水等も含まれる。 Examples of water that can be used as an extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as water that has been subjected to various treatments. Examples of treatments applied to water include purification, heating, sterilization, filtration, ion exchange, osmotic pressure adjustment, buffering, and the like. Therefore, water that can be used as an extraction solvent in this embodiment includes purified water, hot water, ion exchange water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.
抽出溶媒として使用し得る親水性有機溶媒としては、メタノール、エタノール、プロピルアルコール、イソプロピルアルコール等の炭素数１～５の低級脂肪族アルコール；アセトン、メチルエチルケトン等の低級脂肪族ケトン；１，３－ブチレングリコール、プロピレングリコール、グリセリン等の炭素数２～５の多価アルコール等が挙げられる。 Hydrophilic organic solvents that can be used as extraction solvents include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene Examples include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol, and glycerin.
２種以上の極性溶媒の混合液を抽出溶媒として使用する場合、その混合比は適宜調整することができる。例えば、水と低級脂肪族アルコールとの混合液を抽出溶媒として使用する場合には、水と低級脂肪族アルコールとの混合比が９：１～１：９（容量比）であることが好ましく、７：３～２：８（容量比）であることがさらに好ましい。また、水と低級脂肪族ケトンとの混合液を使用する場合には、水と低級脂肪族ケトンとの混合比が９：１～２：８（容量比）であることが好ましく、水と多価アルコールとの混合液を使用する場合には、水と多価アルコールとの混合比が８：２～１：９（容量比）であることが好ましい。 When a mixture of two or more polar solvents is used as an extraction solvent, the mixing ratio can be adjusted as appropriate. For example, when a mixture of water and lower aliphatic alcohol is used as the extraction solvent, the mixing ratio of water and lower aliphatic alcohol is preferably 9:1 to 1:9 (volume ratio), More preferably, the ratio is 7:3 to 2:8 (capacity ratio). In addition, when using a mixed solution of water and lower aliphatic ketone, the mixing ratio of water and lower aliphatic ketone is preferably 9:1 to 2:8 (volume ratio). When using a mixed solution with a polyhydric alcohol, the mixing ratio of water and polyhydric alcohol is preferably 8:2 to 1:9 (volume ratio).
抽出処理は、抽出原料に含まれる可溶性成分を抽出溶媒に溶出させ得る限り特に限定はされず、常法に従って行うことができる。例えば、抽出原料の５～１５倍量（質量比）の抽出溶媒に、抽出原料を浸漬し、常温又は還流加熱下で可溶性成分を抽出させた後、濾過して抽出残渣を除去することにより抽出液を得ることができる。得られた抽出液から溶媒を留去するとペースト状の濃縮物が得られ、この濃縮物をさらに乾燥すると乾燥物が得られる。 The extraction treatment is not particularly limited as long as the soluble components contained in the extraction raw material can be eluted into the extraction solvent, and can be performed according to a conventional method. For example, the extraction raw material is immersed in an extraction solvent of 5 to 15 times the amount (mass ratio) of the extraction raw material, the soluble components are extracted at room temperature or under reflux heating, and then the extraction residue is removed by filtration. liquid can be obtained. When the solvent is distilled off from the obtained extract, a paste-like concentrate is obtained, and when this concentrate is further dried, a dry product is obtained.
〔環境汚染物質によるダメージ抑制剤〕
以上のようにして得られるスターフルーツの果実からの抽出物（以下、単に「スターフルーツ果実抽出物」ということがある。）は、環境汚染物質によるダメージ抑制作用において優れた作用を有しているため、環境汚染物質によるダメージ抑制剤の有効成分として用いることができる。本実施形態に係る、環境汚染物質によるダメージ抑制剤は、医薬品、医薬部外品、化粧品等の幅広い用途に使用することができる。なお、本実施形態に係る、環境汚染物質によるダメージ抑制剤を、それぞれ単に「ダメージ抑制剤」と記載することがある。作用及び用途についても同様である。
[Damage inhibitor caused by environmental pollutants]
The extract from star fruit fruit obtained as described above (hereinafter sometimes simply referred to as "star fruit fruit extract") has an excellent effect in suppressing damage caused by environmental pollutants. Therefore, it can be used as an active ingredient in an agent for suppressing damage caused by environmental pollutants. The agent for suppressing damage caused by environmental pollutants according to this embodiment can be used in a wide range of applications such as pharmaceuticals, quasi-drugs, and cosmetics. Note that the damage inhibitors caused by environmental pollutants according to the present embodiment may be simply referred to as "damage inhibitors." The same applies to actions and uses.
環境汚染物質としては、例えば、水質汚染物質（アルキルベンゼンスルホン酸塩等）、土壌汚染物質（農薬等）、大気汚染物質（ガソリンエンジンの排気ガス、トンネル粉塵等）が挙げられるが、これらに限定されるものではない。より効果的にダメージを抑制できる観点から、大気汚染物質（ガソリンエンジンの排気ガス、トンネル粉塵等）が挙げられる。 Examples of environmental pollutants include, but are not limited to, water pollutants (alkylbenzene sulfonates, etc.), soil pollutants (pesticides, etc.), and air pollutants (gasoline engine exhaust gas, tunnel dust, etc.). It's not something you can do. Air pollutants (gasoline engine exhaust gas, tunnel dust, etc.) can be cited from the perspective of more effectively suppressing damage.
ここで、スターフルーツ果実抽出物が有するダメージ抑制作用は、例えば、環境汚染物質によるタンパク質のカルボニル化抑制作用又は環境汚染物質による炎症関連遺伝子発現上昇抑制作用に基づいて発揮される。ただし、スターフルーツ果実抽出物が有するダメージ抑制作用は、上記タンパク質カルボニル化抑制作用又は上記炎症関連遺伝子の発現上昇抑制作用に基づいて発揮されるダメージ抑制作用に限定されるものではない。 Here, the damage suppressing effect of the star fruit fruit extract is exerted based on, for example, the suppressing effect of protein carbonylation caused by environmental pollutants or the suppressing effect of suppressing the increase in inflammation-related gene expression caused by environmental pollutants. However, the damage suppressing effect of the star fruit fruit extract is not limited to the damage suppressing effect exerted based on the above-mentioned protein carbonylation suppressing effect or the above-mentioned inflammation-related gene expression increase suppressing effect.
環境汚染物質によってタンパク質のカルボニル化が促進され、スターフルーツ果実抽出物によってタンパク質のカルボニル化が抑制される部位としては、皮膚（頭皮含む）、気管支、血管及び毛髪等が挙げられ、特に皮膚については表皮又は真皮等が挙げられるが、より効果的にタンパク質のカルボニル化が抑制できる観点から、表皮の中でも角層が挙げられる。 Areas where protein carbonylation is promoted by environmental pollutants, and protein carbonylation is suppressed by star fruit fruit extract include the skin (including the scalp), bronchi, blood vessels, and hair. Examples include the epidermis and dermis, and among the epidermis, the stratum corneum can be cited from the viewpoint of more effectively suppressing protein carbonylation.
環境汚染物質によって発現が上昇し、スターフルーツ果実抽出物によって当該発現上昇が抑制される炎症関連遺伝子には、例えば、インターロイキン１α（ＩＬ－１α）、シクロオキシゲナーゼ２（ＣＯＸ－２）及びマトリックスメタロプロテアーゼ９（ＭＭＰ－９）等が挙げられる。 Inflammation-related genes whose expression is increased by environmental pollutants and whose expression is suppressed by star fruit fruit extract include, for example, interleukin 1α (IL-1α), cyclooxygenase 2 (COX-2), and matrix metalloprotease. 9 (MMP-9) and the like.
環境汚染物質によって炎症関連遺伝子の発現が上昇し、スターフルーツ果実抽出物によって上記炎症関連遺伝子の発現が抑制される部位としては、皮膚（頭皮含む）、気管支、血管等が挙げられ、特に皮膚については表皮又は真皮等が挙げられるが、より効果的に上記炎症関連遺伝子の発現が抑制できる観点から、表皮の中でも表皮角化細胞が挙げられる。 The expression of inflammation-related genes is increased by environmental pollutants, and the expression of the above-mentioned inflammation-related genes is suppressed by star fruit fruit extract, including the skin (including the scalp), bronchi, and blood vessels. Examples include the epidermis and dermis, and among the epidermis, epidermal keratinocytes are included from the viewpoint of more effectively suppressing the expression of the above-mentioned inflammation-related genes.
スターフルーツ果実抽出物は、環境汚染物質によるタンパク質カルボニル化抑制作用又は環境汚染物質による炎症関連遺伝子発現上昇抑制作用、中でも環境汚染物質によるＩＬ－１α発現上昇抑制作用、環境汚染物質によるＣＯＸ－２発現上昇抑制作用又は環境汚染物質によるＭＭＰ－９発現上昇抑制作用を利用して、環境汚染物質によるタンパク質カルボニル化抑制剤、環境汚染物質によるＩＬ－１α発現上昇抑制剤、環境汚染物質によるＣＯＸ－２発現上昇抑制剤又は環境汚染物質によるＭＭＰ－９発現上昇抑制剤等の有効成分として使用してもよい。 Starfruit fruit extract has the effect of suppressing protein carbonylation caused by environmental pollutants or the increase of inflammation-related gene expression caused by environmental pollutants, especially the effect of suppressing the increase of IL-1α expression caused by environmental pollutants, and the effect of suppressing the increase of COX-2 expression caused by environmental pollutants. Utilizing the effect of suppressing the increase in MMP-9 expression or the effect of suppressing the increase in MMP-9 expression caused by environmental pollutants, it is possible to use an agent that suppresses protein carbonylation caused by environmental pollutants, an agent that suppresses the increase in IL-1α expression caused by environmental pollutants, and an inhibitor of COX-2 expression caused by environmental pollutants. It may be used as an active ingredient in an agent for suppressing the increase in MMP-9 expression or an agent for suppressing the increase in MMP-9 expression caused by environmental pollutants.
本実施形態のダメージ抑制剤は、スターフルーツ果実抽出物のみからなるものでもよいし、スターフルーツ果実抽出物を製剤化したものでもよい。 The damage suppressant of this embodiment may consist only of star fruit fruit extract, or may be a formulation of star fruit fruit extract.
本実施形態のダメージ抑制剤は、デキストリン、シクロデキストリン等の薬学的に許容し得るキャリアーその他任意の助剤を用いて、常法に従い、粉末状、顆粒状、錠剤状、液状等の任意の剤形に製剤化することができる。この際、助剤としては、例えば、賦形剤、結合剤、崩壊剤、滑沢剤、安定剤、矯味・矯臭剤等を用いることができる。ダメージ抑制剤は、他の組成物（例えば、化粧料等）に配合して使用することができるほか、軟膏剤、外用液剤、貼付剤等として使用することができる。 The damage suppressant of this embodiment can be prepared in any form such as powder, granules, tablets, liquid, etc. using a pharmaceutically acceptable carrier such as dextrin or cyclodextrin or any other auxiliary agent according to a conventional method. It can be formulated into a form. In this case, as the auxiliary agent, for example, an excipient, a binder, a disintegrant, a lubricant, a stabilizer, a flavoring agent, etc. can be used. The damage suppressant can be used by being incorporated into other compositions (for example, cosmetics, etc.), and can also be used as an ointment, an external liquid, a patch, and the like.
本実施形態のダメージ抑制剤を製剤化した場合、スターフルーツ果実抽出物の含有量は、特に限定されるものではなく、目的に応じて適宜設定することができる。 When the damage suppressant of this embodiment is formulated, the content of star fruit extract is not particularly limited and can be appropriately set depending on the purpose.
なお、本実施形態のダメージ抑制剤は、必要に応じて、ダメージ抑制作用を有する他の天然抽出物等を、スターフルーツ果実抽出物とともに配合して有効成分として用いることができる。 In addition, the damage suppressant of this embodiment can be used as an active ingredient by blending other natural extracts having a damage suppressing effect with the star fruit fruit extract, if necessary.
本実施形態のダメージ抑制剤の患者に対する投与方法としては、経皮投与、経口投与等が挙げられるが、疾患の種類に応じて、その予防・治療等に好適な方法を適宜選択すればよい。また、本実施形態のダメージ抑制剤の投与量も、疾患の種類、重症度、患者の個人差、投与方法、投与期間等によって適宜増減すればよい。 Methods for administering the damage suppressant of the present embodiment to patients include transdermal administration, oral administration, etc., and methods suitable for prevention, treatment, etc. may be appropriately selected depending on the type of disease. Further, the dosage of the damage suppressing agent of the present embodiment may be appropriately increased or decreased depending on the type of disease, severity, individual differences among patients, administration method, administration period, etc.
本実施形態のダメージ抑制剤は、有効成分であるスターフルーツ果実抽出物の作用を通じて、環境汚染物質によるダメージを抑制することができる。本実施形態のダメージ抑制剤は、かかるスターフルーツ果実抽出物の作用により、環境汚染物質による様々な症状（例えば、気管支炎、喘息、発癌、心疾患、骨や軟骨の破壊、発熱、皮膚の保水力低下、皮膚のバリア機能低下、肌荒れ、炎症及び毛髪の損傷等）を予防、治療又は改善することができる。
ただし、本実施形態のダメージ抑制剤は、これらの用途以外にも、環境汚染物質によるダメージを抑制することに意義のあるすべての用途に用いることができる。
The damage suppressant of the present embodiment can suppress damage caused by environmental pollutants through the action of star fruit fruit extract as an active ingredient. The damage suppressing agent of the present embodiment is effective against various symptoms caused by environmental pollutants (for example, bronchitis, asthma, carcinogenesis, heart disease, bone and cartilage destruction, fever, and skin maintenance) due to the action of the star fruit fruit extract. (lower water power, lower skin barrier function, rough skin, inflammation, hair damage, etc.) can be prevented, treated, or improved.
However, in addition to these uses, the damage suppressant of this embodiment can be used for all uses that are meaningful in suppressing damage caused by environmental pollutants.
例えば、ダメージ抑制剤又は前述した環境汚染物質によるタンパク質カルボニル化抑制剤は、有効成分であるスターフルーツ果実抽出物の作用を通じて、環境汚染物質によるタンパク質のカルボニル化を抑制することができる。本実施形態のダメージ抑制剤は、かかるスターフルーツ果実抽出物の作用により、環境汚染物質によるタンパク質のカルボニル化を抑制し、発癌、発熱、皮膚の保水力低下、皮膚のバリア機能低下、肌荒れ、炎症及び毛髪の損傷等の症状を予防、治療又は改善することができる。 For example, the damage inhibitor or the above-mentioned inhibitor of protein carbonylation caused by environmental pollutants can inhibit protein carbonylation caused by environmental pollutants through the action of star fruit fruit extract, which is an active ingredient. The damage suppressant of this embodiment suppresses protein carbonylation caused by environmental pollutants through the action of the star fruit fruit extract, leading to carcinogenesis, fever, decreased skin water retention capacity, decreased skin barrier function, rough skin, and inflammation. and can prevent, treat or improve symptoms such as hair damage.
例えば、本実施形態のダメージ抑制剤、前述した環境汚染物質によるＩＬ－１α発現上昇抑制剤、環境汚染物質によるＣＯＸ－２発現上昇抑制剤又は環境汚染物質によるＭＭＰ－９発現上昇抑制剤は、有効成分であるスターフルーツ果実抽出物の作用を通じて、環境汚染物質による炎症関連遺伝子の発現上昇を抑制することができる。かかる炎症関連遺伝子には、特にＩＬ－１α、ＣＯＸ－２及びＭＭＰ－９等が含まれる。本実施形態のダメージ抑制剤は、かかるスターフルーツ果実抽出物の作用により、環境汚染物質による炎症関連遺伝子の発現上昇を抑制し、発癌、骨や軟骨の破壊、発熱、皮膚の保水力低下、皮膚のバリア機能低下、肌荒れ及び炎症等の症状を予防、治療又は改善することができる。 For example, the damage inhibitor of the present embodiment, the above-mentioned inhibitor of increase in IL-1α expression caused by environmental pollutants, inhibitor of increase in COX-2 expression caused by environmental pollutants, or inhibitor of increase in MMP-9 expression caused by environmental pollutants are effective. Through the action of star fruit fruit extract, which is an ingredient, it is possible to suppress the increase in expression of inflammation-related genes caused by environmental pollutants. Such inflammation-related genes include IL-1α, COX-2, and MMP-9, among others. The damage suppressing agent of this embodiment suppresses the increase in the expression of inflammation-related genes caused by environmental pollutants through the action of the star fruit fruit extract, thereby causing carcinogenesis, destruction of bones and cartilage, fever, decreased water retention capacity of the skin, and It is possible to prevent, treat, or improve symptoms such as decreased barrier function, rough skin, and inflammation.
また、本実施形態のダメージ抑制剤は、ダメージ抑制作用において優れた作用を有するため、例えば、皮膚外用剤や頭皮外用剤等の経皮用外用剤等に配合するのに好適である。この場合に、スターフルーツ果実抽出物をそのまま配合してもよいし、スターフルーツ果実抽出物から製剤化したダメージ抑制剤を配合してもよい。 Furthermore, the damage suppressant of the present embodiment has an excellent damage suppressing effect and is therefore suitable for being incorporated into transdermal external preparations such as external preparations for skin and external preparations for scalp. In this case, star fruit fruit extract may be blended as it is, or a damage suppressant formulated from star fruit fruit extract may be blended.
ここで、経皮用外用剤としては、その区分に制限はなく、後述する化粧料のほか、経皮的に使用される医薬部外品、医薬品等を幅広く含むものである。 Here, the category of transdermal external preparations is not limited and includes a wide range of cosmetics, which will be described later, as well as quasi-drugs, pharmaceuticals, etc. that are used transdermally.
また、本実施形態のダメージ抑制剤は、優れたダメージ抑制作用を有するので、これらの作用機構に関する研究のための試薬としても好適に利用することができる。 Moreover, since the damage suppressing agent of this embodiment has an excellent damage suppressing effect, it can also be suitably used as a reagent for research on the mechanism of these effects.
〔環境汚染物質によるダメージ抑制用化粧料〕
スターフルーツ果実抽出物は、ダメージ抑制作用において優れた作用を有しているため、化粧料に配合するのに好適である。この場合、スターフルーツ果実抽出物をそのまま配合してもよいし、スターフルーツ果実抽出物から製剤化したダメージ抑制剤を配合してもよい。
[Cosmetics for suppressing damage caused by environmental pollutants]
Starfruit fruit extract has an excellent damage-inhibiting effect and is therefore suitable for inclusion in cosmetics. In this case, star fruit fruit extract may be blended as it is, or a damage suppressant formulated from star fruit fruit extract may be blended.
スターフルーツ果実抽出物又は上記ダメージ抑制剤を配合することにより、ダメージ抑制用途に好適な化粧料とすることができる。 By blending star fruit fruit extract or the above-mentioned damage suppressing agent, a cosmetic suitable for damage suppressing purposes can be obtained.
スターフルーツ果実抽出物、又は上記ダメージ抑制剤を配合し得る化粧料の種類は特に限定されるものではなく、皮膚化粧料、頭髪化粧料等とすることができ、例えば、軟膏、クリーム、乳液、化粧水、ローション、ジェル、美容オイル、パック、ファンデーション、ヘアートニック、ヘアーローション、ヘアークリーム、整髪剤、シャンプー、リンス、トリートメント等が挙げられる。 The type of cosmetics that can be blended with star fruit fruit extract or the damage suppressant is not particularly limited, and may be skin cosmetics, hair cosmetics, etc., such as ointments, creams, milky lotions, etc. Examples include lotion, lotion, gel, beauty oil, pack, foundation, hair tonic, hair lotion, hair cream, hair conditioner, shampoo, conditioner, treatment, etc.
スターフルーツ果実抽出物、又は上記ダメージ抑制剤を化粧料に配合する場合、その配合量は、化粧料の種類に応じて適宜調整することができるが、好適な配合率は、約０．０００１～１０質量％であり、特に好適な配合率は、標準的な抽出物に換算して約０．００１～１質量％である。 When blending star fruit fruit extract or the above-mentioned damage inhibitor into cosmetics, the blending amount can be adjusted as appropriate depending on the type of cosmetic, but the preferred blending ratio is approximately 0.0001~ 10% by weight, and a particularly preferred loading is about 0.001 to 1% by weight, calculated as standard extract.
本実施形態のダメージ抑制用化粧料は、スターフルーツ果実抽出物が有するダメージ抑制作用を妨げない限り、通常の化粧料の製造に用いられる主剤、助剤又はその他の成分、例えば、収斂剤、殺菌・抗菌剤、美白剤、紫外線吸収剤、保湿剤、細胞賦活剤、消炎・抗アレルギー剤、抗酸化・活性酸素除去剤、油脂類、ロウ類、炭化水素類、脂肪酸類、アルコール類、エステル類、界面活性剤、香料等を併用することができる。このように併用することで、より一般性のある製品となり、また、併用された他の有効成分との間の相乗作用が通常期待される以上の優れた効果をもたらすことがある。 The damage-inhibiting cosmetic of the present embodiment may contain base ingredients, auxiliary agents, or other ingredients used in the production of ordinary cosmetics, such as astringents, sterilizers, etc., as long as they do not interfere with the damage-inhibiting effect of the star fruit fruit extract.・Antibacterial agents, whitening agents, ultraviolet absorbers, humectants, cell activators, anti-inflammatory/anti-allergic agents, antioxidants/active oxygen removers, oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters , a surfactant, a fragrance, etc. can be used in combination. By using them in combination in this way, the product becomes more general in use, and the synergistic effect between them and other active ingredients used in combination may bring about better effects than would normally be expected.
本実施形態のダメージ抑制用化粧料は、スターフルーツ果実抽出物が有するダメージ抑制作用を通じて、環境汚染物質による症状（例えば、皮膚の保水力低下、皮膚のバリア機能低下、肌荒れ、炎症及び毛髪の損傷等）の予防、治療又は改善等をすることができる。 The damage-suppressing cosmetic of the present embodiment can reduce symptoms caused by environmental pollutants (e.g., decreased skin water retention, decreased skin barrier function, rough skin, inflammation, and hair damage) through the damage-inhibiting action of star fruit extract. etc.) can be prevented, treated, or improved.
〔環境汚染物質によるダメージ抑制用飲食品〕
スターフルーツ果実抽出物は、ダメージ抑制作用において優れた作用を有しているため、飲食品に配合するのに好適である。この場合、スターフルーツ果実抽出物をそのまま配合してもよいし、スターフルーツ果実抽出物から製剤化したダメージ抑制剤を配合してもよい。スターフルーツ果実抽出物又は上記ダメージ抑制剤を配合することにより、ダメージ抑制用途に好適な飲食品とすることができる。
[Food and beverages for reducing damage caused by environmental pollutants]
Starfruit fruit extract has an excellent damage-inhibiting effect, and is therefore suitable for inclusion in foods and drinks. In this case, star fruit fruit extract may be blended as it is, or a damage suppressant formulated from star fruit fruit extract may be blended. By blending star fruit fruit extract or the above-mentioned damage suppressing agent, a food or drink suitable for damage suppressing purposes can be obtained.
ここで、飲食品とは、人の健康に危害を加えるおそれが少なく、通常の社会生活において、経口又は消化管投与により摂取されるものをいい、行政区分上の食品、医薬品、医薬部外品等の区分に制限されるものではない。したがって、本実施形態における「飲食品」は、経口的に摂取される一般食品、健康食品（機能性飲食品）、保健機能食品（特定保健用食品，栄養機能食品）、医薬部外品、医薬品等を幅広く含むものである。本実施形態に係る飲食品は、当該飲食品又はその包装に、スターフルーツ果実抽出物が有する好ましい作用を表示することのできる飲食品であることが好ましく、保健機能食品（特定保健用食品，機能性表示食品、栄養機能食品）、医薬部外品及び医薬品であることが特に好ましい。 Here, food and beverages refer to foods that have little risk of harming human health and are ingested orally or through gastrointestinal administration in normal social life, and are administratively classified foods, drugs, and quasi-drugs. It is not limited to the following categories. Therefore, "food and drink" in this embodiment refers to general food that is orally ingested, health food (functional food and drink), food with health claims (food for specified health uses, food with nutritional function), quasi-drugs, and pharmaceuticals. It includes a wide range of things such as The food/beverage product according to this embodiment is preferably a food/beverage product that can display the favorable effects of star fruit fruit extract on the food/beverage product or its packaging, and is preferably a food/beverage product with health claims (food for specified health uses, food with functional health claims, etc.). Particularly preferred are foods with gender indication, foods with nutritional function claims), quasi-drugs, and pharmaceuticals.
スターフルーツ果実抽出物、又はスターフルーツ果実抽出物から製剤化したダメージ抑制剤を飲食品に配合する場合、それらにおける有効成分の配合量は、使用目的、症状、性別等を考慮して適宜変更することができるが、添加対象となる飲食品の一般的な摂取量を考慮して、成人１日あたりの抽出物摂取量が約１～１０００ｍｇになるようにするのが好ましい。なお、添加対象飲食品が顆粒状、錠剤状又はカプセル状の飲食品の場合、スターフルーツ果実抽出物、又はスターフルーツ果実抽出物から製剤化したダメージ抑制剤の添加量は、添加対象飲食品に対して通常０．１～１００質量％であり、好ましくは５～１００質量％である。 When adding starfruit fruit extract or a damage suppressant formulated from starfruit fruit extract to foods and drinks, the amount of active ingredients in them should be changed as appropriate, taking into consideration the purpose of use, symptoms, gender, etc. However, taking into consideration the general intake of food and beverages to which it is added, it is preferable that the intake of the extract is approximately 1 to 1000 mg per day for adults. In addition, if the food/drink to which the additive is added is in the form of granules, tablets, or capsules, the amount of star fruit fruit extract or damage suppressant formulated from star fruit fruit extract shall be the same as the food/beverage to which the additive is added. The amount is usually 0.1 to 100% by weight, preferably 5 to 100% by weight.
本実施形態のダメージ抑制用飲食品は、スターフルーツ果実抽出物をその活性を妨げないような任意の飲食品に配合したものであってもよいし、スターフルーツ果実抽出物を主成分とする栄養補助食品であってもよい。 The damage-suppressing food/beverage product of the present embodiment may be one in which star fruit fruit extract is blended into any food/beverage product that does not impede its activity, or a nutritional product containing star fruit fruit extract as a main component. It may also be a supplementary food.
本実施形態のダメージ抑制用飲食品を製造する際には、例えば、デキストリン、デンプン等の糖類；ゼラチン、大豆タンパク、トウモロコシタンパク等のタンパク質；アラニン、グルタミン、イソロイシン等のアミノ酸類；セルロース、アラビアゴム等の多糖類；大豆油、中鎖脂肪酸トリグリセリド等の油脂類などの任意の助剤を添加して任意の形状の飲食品にすることができる。 When producing the damage-preventing food and drink of this embodiment, for example, sugars such as dextrin and starch; proteins such as gelatin, soybean protein, and corn protein; amino acids such as alanine, glutamine, and isoleucine; cellulose, gum arabic, etc. By adding arbitrary auxiliary agents such as polysaccharides such as soybean oil and fats and oils such as medium-chain fatty acid triglycerides, foods and drinks of any shape can be made.
スターフルーツ果実抽出物を配合し得る飲食品は特に限定されないが、その具体例としては、清涼飲料、炭酸飲料、栄養飲料、果実飲料、乳酸飲料等の飲料（これらの飲料の濃縮原液及び調整用粉末を含む）；アイスクリーム、アイスシャーベット、かき氷等の冷菓；そば、うどん、はるさめ、ぎょうざの皮、しゅうまいの皮、中華麺、即席麺等の麺類；飴、チューインガム、キャンディー、ガム、チョコレート、錠菓、スナック菓子、ビスケット、ゼリー、ジャム、クリーム、焼き菓子等の菓子類；かまぼこ、ハム、ソーセージ等の水産・畜産加工食品；加工乳、発酵乳等の乳製品；サラダ油、てんぷら油、マーガリン、マヨネーズ、ショートニング、ホイップクリーム、ドレッシング等の油脂及び油脂加工食品；ソース、たれ等の調味料；スープ、シチュー、サラダ、惣菜、漬物；その他種々の形態の健康・栄養補助食品；錠剤、カプセル剤、ドリンク剤などが挙げられる。これらの飲食品にスターフルーツ果実抽出物を配合するときには、通常用いられる補助的な原料や添加物を併用することができる。 Foods and drinks that can contain star fruit fruit extract are not particularly limited. (including powder); Frozen desserts such as ice cream, ice sorbet, and shaved ice; Noodles such as soba, udon, Harusame, gyoza skin, shumai skin, Chinese noodles, and instant noodles; candy, chewing gum, candies, gum, chocolate, and tablets Confectionery such as cakes, snacks, biscuits, jellies, jams, creams, and baked goods; Fishery and livestock processed foods such as kamaboko, ham, and sausage; Dairy products such as processed milk and fermented milk; Salad oil, tempura oil, margarine, and mayonnaise , fats and oils and processed foods such as shortening, whipped cream, and dressings; seasonings such as sauces and sauces; soups, stews, salads, side dishes, and pickles; and various other forms of health and nutritional supplements; tablets, capsules, and drinks. Examples include agents. When blending star fruit fruit extract into these foods and drinks, commonly used auxiliary raw materials and additives can be used in combination.
本実施形態のダメージ抑制用飲食品は、スターフルーツ果実抽出物が有するダメージ抑制作用を通じて、環境汚染物質による症状（例えば、気管支炎、喘息、発癌、心疾患、骨や軟骨の破壊、発熱、皮膚の保水力低下、皮膚のバリア機能低下、肌荒れ、炎症及び毛髪の損傷等）の予防、治療又は改善等をすることができる。 The damage-suppressing food and drink of the present embodiment can reduce symptoms caused by environmental pollutants (e.g., bronchitis, asthma, carcinogenesis, heart disease, bone and cartilage destruction, fever, skin irritation, etc.) through the damage-suppressing effect of star fruit extract. It is possible to prevent, treat, or improve problems such as decreased water retention capacity, decreased skin barrier function, rough skin, inflammation, and hair damage.
なお、本実施形態のダメージ抑制剤、ダメージ抑制用化粧料及びダメージ抑制用飲食品は、ヒトに対して好適に適用されるものであるが、それぞれの作用効果が奏される限り、ヒト以外の動物（例えば，マウス，ラット，ハムスター，イヌ，ネコ，ウシ，ブタ，サル等）に対して適用することもできる。 Although the damage suppressing agent, damage suppressing cosmetic, and damage suppressing food and beverage of this embodiment are preferably applied to humans, they may also be applied to non-humans as long as the respective effects are achieved. It can also be applied to animals (eg, mice, rats, hamsters, dogs, cats, cows, pigs, monkeys, etc.).
以下、試験例を示し、本発明を具体的に説明するが、本発明は下記の各例に何ら制限されるものではない。 Hereinafter, the present invention will be specifically explained using test examples, but the present invention is not limited to the following examples.
〔製造例〕
スターフルーツ（学名：Averrhoa carambola L.）の果実の粗粉砕物１００ｇを抽出溶媒１５００ｍｌに投入し、４５～５５℃で４時間、穏やかに攪拌しながら抽出を行った。その後ろ過し、ろ液を６０℃で減圧下にて濃縮し、さらに減圧乾燥機で乾燥してスターフルーツの果実抽出物を得た。抽出物の収率は表１のとおりであった。
[Manufacturing example]
100 g of coarsely ground fruit of star fruit (scientific name: Averrhoa carambola L.) was added to 1500 ml of extraction solvent, and extraction was performed at 45 to 55° C. for 4 hours with gentle stirring. Thereafter, it was filtered, and the filtrate was concentrated under reduced pressure at 60° C. and further dried in a vacuum drier to obtain a star fruit fruit extract. The yield of the extract was as shown in Table 1.
〔試験例１〕環境汚染物質によるタンパク質カルボニル化抑制作用
スターフルーツ果実抽出物（試料１）について、以下のようにして、環境汚染物質による角層タンパク質カルボニル化抑制作用を試験した。
[Test Example 1] Effect of inhibiting protein carbonylation caused by environmental pollutants The star fruit fruit extract (sample 1) was tested for its effect of suppressing stratum corneum protein carbonylation caused by environmental pollutants as follows.
非露光部である上腕内側部の角層を、角質チェッカー（アサヒバイオメッド社製）を用いてテープストリッピング法により剥離・回収した。角層が付着した角質チェッカー（サンプル）を、被験試料（試料１，試料濃度は下記表２を参照）の水溶液１ｍＬに浸漬し、３７℃で、２４時間処理した。なお、コントロールとして、試料無添加の水を用いて同様に処理した。処理後、サンプルを取り出して風乾させた。次いで、チャック付きのビニール袋に採取したガソリンエンジンの排気ガスに曝露させ、室温にて２４時間反応させた。なお、ブランクとして、室内空気に曝露させたサンプルも用意した。得られたサンプルについて、角層カルボニル化のレベルを以下の方法で評価した。 The stratum corneum on the inner side of the upper arm, which is a non-exposed area, was peeled off and collected by a tape stripping method using a keratin checker (manufactured by Asahi Biomed). A corneum checker (sample) with attached stratum corneum was immersed in 1 mL of an aqueous solution of a test sample (sample 1, see Table 2 below for sample concentration) and treated at 37° C. for 24 hours. In addition, as a control, the same treatment was performed using water to which no sample was added. After processing, the samples were removed and air-dried. Next, it was exposed to gasoline engine exhaust gas collected in a plastic bag with a zipper, and reacted at room temperature for 24 hours. A sample exposed to room air was also prepared as a blank. Regarding the obtained samples, the level of stratum corneum carbonylation was evaluated by the following method.
２０μmol/Ｌ蛍光ヒドラジド（fluorescein-5-thiosemicarbazide，AnaSpec社製）を含有する０．１mol/Ｌ ＭＥＳ緩衝液（ｐＨ５．５）にサンプルを浸漬し、室温・遮光条件下で１時間反応させ、角層タンパク質のカルボニル基をラベル化した。反応終了後、ＰＢＳ（－）緩衝液１ｍＬで２回洗浄し、蛍光顕微鏡（BZ-X800，キーエンス社製）にて画像撮影した。得られた画像を画像解析ソフト（BZ-X800 analyzer，キーエンス社製）を用いて解析し、角層面積あたりの蛍光輝度をカルボニル化レベルとした。得られた結果から、下記式により角層タンパク質カルボニル化抑制率（％）を算出した。 The sample was immersed in 0.1 mol/L MES buffer (pH 5.5) containing 20 μmol/L fluorescent hydrazide (fluorescein-5-thiosemicarbazide, manufactured by AnaSpec), reacted for 1 hour at room temperature and shielded from light, and then The carbonyl groups of the layer proteins were labeled. After the reaction was completed, the plate was washed twice with 1 mL of PBS(-) buffer, and an image was taken using a fluorescence microscope (BZ-X800, manufactured by Keyence Corporation). The obtained images were analyzed using image analysis software (BZ-X800 analyzer, manufactured by Keyence Corporation), and the fluorescence brightness per stratum corneum area was defined as the carbonylation level. From the obtained results, the stratum corneum protein carbonylation inhibition rate (%) was calculated using the following formula.
角層タンパク質カルボニル化抑制率（％）＝｛１－（Ａ－Ｃ）／（Ｂ－Ｃ）｝×１００
式中の各項はそれぞれ以下を表す。
Ａ：排気ガス処理・被験試料処理のカルボニル化レベル
Ｂ：排気ガス処理・被験試料無処理（コントロール）のカルボニル化レベル
Ｃ：排気ガス無処理・被験試料無処理（ブランク）のカルボニル化レベル
結果を表２に示す。
Stratum corneum protein carbonylation inhibition rate (%) = {1-(A-C)/(B-C)}×100
Each term in the formula represents the following.
A: Carbonylation level with exhaust gas treatment/test sample treatment B: Carbonylation level with exhaust gas treatment/no test sample treatment (control) C: Carbonylation level without exhaust gas treatment/no test sample treatment (blank) Results It is shown in Table 2.
表２に示すように、スターフルーツ果実抽出物（試料１）は、排気ガスによる角層タンパク質のカルボニル化を効果的に抑制した。 As shown in Table 2, the star fruit fruit extract (Sample 1) effectively suppressed the carbonylation of stratum corneum proteins caused by exhaust gas.
〔試験例２〕環境汚染物質によるＩＬ－１αの発現上昇抑制作用
スターフルーツ果実抽出物（試料１）について、以下のようにして、環境汚染物質によるＩＬ－１αの発現上昇抑制作用を試験した。
[Test Example 2] Effect of suppressing the increase in IL-1α expression caused by environmental pollutants Star fruit fruit extract (Sample 1) was tested for its effect of suppressing the increase in IL-1α expression caused by environmental pollutants as follows.
正常ヒト新生児表皮角化細胞（ＮＨＥＫ）を、正常ヒト表皮角化細胞用増殖培地（ＫＧＭ）を用いて培養した後、トリプシン処理により細胞を回収した。回収した細胞を２０×１０４cells/ｍＬの細胞密度になるようにＫＧＭ培地で希釈した後、３５ｍｍシャーレに２ｍＬずつ播種し（４０×１０４cells/シャーレ）、２４時間培養した。 Normal human neonatal epidermal keratinocytes (NHEK) were cultured using normal human epidermal keratinocyte growth medium (KGM), and then the cells were collected by trypsin treatment. The collected cells were diluted with KGM medium to a cell density of 20×10 4 cells/mL, then seeded in 2 mL portions into 35 mm petri dishes (40×10 4 cells/petri dish), and cultured for 24 hours.
培養後に培地を基礎培地（ＫＢＭ）に交換し、２４時間培養した。培養後、培養液を除去し、被験試料（試料１，試料濃度は下記表３を参照）と、多環芳香族炭化水素類・有害元素分析用のトンネル粉塵（産業技術総合研究所；認証標準物質 ＮＭＩＪＣＲＭ 7308-a，終濃度２０μｇ／ｍＬ）とを溶解したＫＢＭ培地を各シャーレに２ｍＬずつ添加し、２４時間培養した。なお、コントロールとして、トンネル粉塵無添加のＫＢＭ培地又は被験試料無添加のＫＢＭ培地を用いて同様に培養した。培養後、培地を除去し、ＩＳＯＧＥＮ II（ニッポンジーン社製，Cat. No. 311-07361）にて総ＲＮＡを抽出し、それぞれのＲＮＡ量を分光光度計にて測定し、２００ｎｇ/μＬになるように総ＲＮＡを調製した。 After culturing, the medium was replaced with basal medium (KBM) and cultured for 24 hours. After culturing, the culture solution was removed and the test sample (sample 1, see Table 3 below for sample concentration) and tunnel dust for analysis of polycyclic aromatic hydrocarbons and harmful elements (National Institute of Advanced Industrial Science and Technology; certification standard) were prepared. 2 mL of KBM medium in which the substance NMIJCRM 7308-a (final concentration 20 μg/mL) was dissolved was added to each Petri dish and cultured for 24 hours. As a control, culture was performed in the same manner using KBM medium without addition of tunnel dust or KBM medium without addition of test sample. After culturing, remove the medium, extract total RNA using ISOGEN II (manufactured by Nippon Gene Co., Ltd., Cat. No. 311-07361), measure the amount of each RNA using a spectrophotometer, and adjust the amount to 200 ng/μL. Total RNA was prepared.
この総ＲＮＡを鋳型とし、ＩＬ－１α及び内部標準であるＧＡＰＤＨについて、ｍＲＮＡの発現量を測定した。検出はリアルタイムＰＣＲ装置Smart Cycler（Cepheid社製）を用いて、TaKaRa SYBR PrimeScript RT-PCR kit（Perfect Real Time）（タカラバイオ社製，code No. RR063A）によるリアルタイム２ Step ＲＴ－ＰＣＲ反応により行った。ＩＬ－１α ｍＲＮＡの発現量は、「トンネル粉塵無添加・被験試料無添加」、「トンネル粉塵添加・被験試料無添加」及び「トンネル粉塵添加・被験試料添加」にてそれぞれ培養した細胞から調製した総ＲＮＡ標品を基にして、ＧＡＰＤＨの値で補正値を求めた。得られた値から、下記式によりＩＬ－１α ｍＲＮＡ発現上昇抑制率（％）を算出した。 Using this total RNA as a template, the expression levels of mRNA were measured for IL-1α and GAPDH as an internal standard. Detection was performed using a real-time PCR device Smart Cycler (manufactured by Cepheid) and a real-time 2 Step RT-PCR reaction using TaKaRa SYBR PrimeScript RT-PCR kit (Perfect Real Time) (manufactured by Takara Bio, code No. RR063A). . The expression level of IL-1α mRNA was determined from cells cultured with "no addition of tunnel dust and no addition of test sample", "addition of tunnel dust and no addition of test sample", and "addition of tunnel dust and addition of test sample". Based on the total RNA preparation, a correction value was determined using the GAPDH value. From the obtained values, the inhibition rate (%) of increase in IL-1α mRNA expression was calculated using the following formula.
ＩＬ－１α ｍＲＮＡ発現上昇抑制率（％）＝｛（Ａ－Ｂ）－（Ａ－Ｃ）｝／（Ａ－Ｂ）×１００
式中の各項はそれぞれ以下を表す。
Ａ：トンネル粉塵無添加・被験試料無添加での補正値
Ｂ：トンネル粉塵添加・被験試料無添加での補正値
Ｃ：トンネル粉塵添加・被験試料添加での補正値
結果を表３に示す。
Suppression rate of increase in IL-1α mRNA expression (%) = {(AB)-(AC)}/(AB)×100
Each term in the formula represents the following.
A: Correction value without addition of tunnel dust and test sample B: Correction value with addition of tunnel dust and no test sample C: Correction value with addition of tunnel dust and test sample The results are shown in Table 3.
表３に示すように、スターフルーツ果実抽出物（試料１）は、環境汚染物質によるＩＬ－１αの発現上昇抑制作用として優れた作用を有することが確認された。 As shown in Table 3, it was confirmed that the star fruit fruit extract (Sample 1) has an excellent effect of suppressing the increase in IL-1α expression caused by environmental pollutants.
〔試験例３〕環境汚染物質によるＣＯＸ－２の発現上昇抑制作用
スターフルーツ果実抽出物（試料１）について、以下のようにして、環境汚染物質によるＣＯＸ－２の発現上昇抑制作用を試験した。
[Test Example 3] Effect of suppressing the increase in COX-2 expression caused by environmental pollutants The star fruit fruit extract (Sample 1) was tested for its effect of suppressing the increase in COX-2 expression caused by environmental pollutants as follows.
正常ヒト新生児表皮角化細胞（ＮＨＥＫ）を、正常ヒト表皮角化細胞用増殖培地（ＫＧＭ）を用いて培養した後、トリプシン処理により細胞を回収した。回収した細胞を２０×１０４cells/ｍＬの細胞密度になるようにＫＧＭ培地で希釈した後、３５ｍｍシャーレに２ｍＬずつ播種し（４０×１０４cells/シャーレ）、２４時間培養した。 Normal human neonatal epidermal keratinocytes (NHEK) were cultured using normal human epidermal keratinocyte growth medium (KGM), and then the cells were collected by trypsin treatment. The collected cells were diluted with KGM medium to a cell density of 20×10 4 cells/mL, then seeded in 2 mL portions in a 35 mm Petri dish (40×10 4 cells/Petri dish), and cultured for 24 hours.
培養後に培地を基礎培地（ＫＢＭ）に交換し、２４時間培養した。培養後、培養液を除去し、被験試料（試料１，試料濃度は下記表４を参照）と、多環芳香族炭化水素類・有害元素分析用のトンネル粉塵（産業技術総合研究所；認証標準物質 ＮＭＩＪＣＲＭ 7308-a，終濃度２０μｇ／ｍＬ）とを溶解したＫＢＭ培地を各シャーレに２ｍＬずつ添加し、２４時間培養した。なお、コントロールとして、トンネル粉塵無添加のＫＢＭ培地又は試料無添加のＫＢＭ培地を用いて同様に培養した。培養後、培地を除去し、ＩＳＯＧＥＮ II（ニッポンジーン社製，Cat. No. 311-07361）にて総ＲＮＡを抽出し、それぞれのＲＮＡ量を分光光度計にて測定し、２００ｎｇ/μＬになるように総ＲＮＡを調製した。 After culturing, the medium was replaced with basal medium (KBM) and cultured for 24 hours. After culturing, the culture solution was removed and the test sample (sample 1, see Table 4 below for sample concentration) and tunnel dust for analysis of polycyclic aromatic hydrocarbons and harmful elements (National Institute of Advanced Industrial Science and Technology; certification standard) were prepared. 2 mL of KBM medium in which the substance NMIJCRM 7308-a (final concentration 20 μg/mL) was dissolved was added to each Petri dish and cultured for 24 hours. As a control, KBM medium without tunnel dust or KBM medium without sample was cultured in the same manner. After culturing, remove the medium, extract total RNA using ISOGEN II (manufactured by Nippon Gene Co., Ltd., Cat. No. 311-07361), measure the amount of each RNA using a spectrophotometer, and adjust the amount to 200 ng/μL. Total RNA was prepared.
この総ＲＮＡを鋳型とし、ＣＯＸ－２及び内部標準であるＧＡＰＤＨについて、ｍＲＮＡの発現量を測定した。検出はリアルタイムＰＣＲ装置Smart Cycler（Cepheid社製）を用いて、TaKaRa SYBR PrimeScript RT-PCR kit（Perfect Real Time）（タカラバイオ社製，code No. RR063A）によるリアルタイム２ Step ＲＴ－ＰＣＲ反応により行った。ＣＯＸ－２ ｍＲＮＡの発現量は、「トンネル粉塵無添加・被験試料無添加」、「トンネル粉塵添加・被験試料無添加」及び「トンネル粉塵添加・被験試料添加」にてそれぞれ培養した細胞から調製した総ＲＮＡ標品を基にして、ＧＡＰＤＨの値で補正値を求めた。得られた値から、下記式によりＣＯＸ－２ ｍＲＮＡ発現上昇抑制率（％）を算出した。 Using this total RNA as a template, the expression levels of mRNA for COX-2 and GAPDH as an internal standard were measured. Detection was performed using a real-time PCR device Smart Cycler (manufactured by Cepheid) and a real-time 2 Step RT-PCR reaction using TaKaRa SYBR PrimeScript RT-PCR kit (Perfect Real Time) (manufactured by Takara Bio, code No. RR063A). . The expression level of COX-2 mRNA was determined from cells cultured with "no addition of tunnel dust and no addition of test sample", "addition of tunnel dust and no addition of test sample", and "addition of tunnel dust and addition of test sample". Based on the total RNA preparation, a correction value was determined using the GAPDH value. From the obtained values, the inhibition rate (%) of increase in COX-2 mRNA expression was calculated using the following formula.
ＣＯＸ－２ ｍＲＮＡ発現上昇抑制率（％）＝｛（Ａ－Ｂ）－（Ａ－Ｃ）｝／（Ａ－Ｂ）×１００
式中の各項はそれぞれ以下を表す。
Ａ：トンネル粉塵無添加・被験試料無添加での補正値
Ｂ：トンネル粉塵添加・被験試料無添加での補正値
Ｃ：トンネル粉塵添加・被験試料添加での補正値
結果を表４に示す。
COX-2 mRNA expression increase suppression rate (%) = {(AB)-(AC)}/(AB)×100
Each term in the formula represents the following.
A: Correction value without addition of tunnel dust and test sample B: Correction value with addition of tunnel dust and no test sample C: Correction value with addition of tunnel dust and test sample The results are shown in Table 4.
表４に示すように、スターフルーツ果実抽出物（試料１）は、環境汚染物質によるＣＯＸ－２の発現上昇抑制作用として優れた作用を有することが確認された。 As shown in Table 4, it was confirmed that the star fruit fruit extract (Sample 1) has an excellent effect of suppressing the increase in COX-2 expression caused by environmental pollutants.
〔試験例４〕環境汚染物質によるＭＭＰ－９の発現上昇抑制作用
スターフルーツ果実抽出物（試料１）について、以下のようにして、環境汚染物質によるＭＭＰ－９の発現上昇抑制作用を試験した。
[Test Example 4] Effect of suppressing the increase in MMP-9 expression caused by environmental pollutants The star fruit fruit extract (Sample 1) was tested for its effect of suppressing the increase in MMP-9 expression caused by environmental pollutants as follows.
正常ヒト新生児表皮角化細胞（ＮＨＥＫ）を、正常ヒト表皮角化細胞用増殖培地（ＫＧＭ）を用いて培養した後、トリプシン処理により細胞を回収した。回収した細胞を２０×１０４cells/ｍＬの細胞密度になるようにＫＧＭ培地で希釈した後、３５ｍｍシャーレに２ｍＬずつ播種し（４０×１０４cells/シャーレ）、２４時間培養した。 Normal human neonatal epidermal keratinocytes (NHEK) were cultured using normal human epidermal keratinocyte growth medium (KGM), and then the cells were collected by trypsin treatment. The collected cells were diluted with KGM medium to a cell density of 20×10 4 cells/mL, then seeded in 2 mL portions into 35 mm petri dishes (40×10 4 cells/petri dish), and cultured for 24 hours.
培養後に培地を基礎培地（ＫＢＭ）に交換し、２４時間培養した。培養後、培養液を除去し、被験試料（試料１，試料濃度は下記表５を参照）と、多環芳香族炭化水素類・有害元素分析用のトンネル粉塵（産業技術総合研究所；認証標準物質 ＮＭＩＪＣＲＭ 7308-a，終濃度２０μｇ／ｍＬ）とを溶解したＫＢＭ培地を各シャーレに２ｍＬずつ添加し、２４時間培養した。なお、コントロールとして、トンネル粉塵無添加のＫＢＭ培地又は試料無添加のＫＢＭ培地を用いて同様に培養した。培養後、培地を除去し、ＩＳＯＧＥＮ II（ニッポンジーン社製，Cat. No. 311-07361）にて総ＲＮＡを抽出し、それぞれのＲＮＡ量を分光光度計にて測定し、２００ｎｇ/μＬになるように総ＲＮＡを調製した。 After culturing, the medium was replaced with basal medium (KBM) and cultured for 24 hours. After culturing, remove the culture solution and remove the test sample (Sample 1, see Table 5 below for sample concentration) and tunnel dust for analysis of polycyclic aromatic hydrocarbons and harmful elements (National Institute of Advanced Industrial Science and Technology; certification standard). 2 mL of KBM medium in which the substance NMIJCRM 7308-a (final concentration 20 μg/mL) was dissolved was added to each Petri dish and cultured for 24 hours. As a control, KBM medium without tunnel dust or KBM medium without sample was cultured in the same manner. After culturing, remove the medium, extract total RNA using ISOGEN II (manufactured by Nippon Gene Co., Ltd., Cat. No. 311-07361), measure the amount of each RNA using a spectrophotometer, and adjust the amount to 200 ng/μL. Total RNA was prepared.
この総ＲＮＡを鋳型とし、ＭＭＰ－９及び内部標準であるＧＡＰＤＨについて、ｍＲＮＡの発現量を測定した。検出はリアルタイムＰＣＲ装置Smart Cycler（Cepheid社製）を用いて、TaKaRa SYBR PrimeScript RT-PCR kit（Perfect Real Time）（タカラバイオ社製，code No. RR063A）によるリアルタイム２ Step ＲＴ－ＰＣＲ反応により行った。ＭＭＰ－９ ｍＲＮＡの発現量は、「トンネル粉塵無添加・被験試料無添加」、「トンネル粉塵添加・被験試料無添加」及び「トンネル粉塵添加・被験試料添加」にてそれぞれ培養した細胞から調製した総ＲＮＡ標品を基にして、ＧＡＰＤＨの値で補正値を求めた。得られた値から、下記式によりＭＭＰ－９ ｍＲＮＡ発現上昇抑制率（％）を算出した。 Using this total RNA as a template, the expression levels of mRNA were measured for MMP-9 and GAPDH as an internal standard. Detection was performed using a real-time PCR device Smart Cycler (manufactured by Cepheid) and a real-time 2 Step RT-PCR reaction using TaKaRa SYBR PrimeScript RT-PCR kit (Perfect Real Time) (manufactured by Takara Bio, code No. RR063A). . The expression level of MMP-9 mRNA was determined from cells cultured with "no addition of tunnel dust/no test sample", "addition of tunnel dust/no test sample", and "addition of tunnel dust/no test sample". Based on the total RNA preparation, a correction value was determined using the GAPDH value. From the obtained values, the inhibition rate (%) of increase in MMP-9 mRNA expression was calculated using the following formula.
ＭＭＰ－９ ｍＲＮＡ発現上昇抑制率（％）＝｛（Ａ－Ｂ）－（Ａ－Ｃ）｝／（Ａ－Ｂ）×１００
式中の各項はそれぞれ以下を表す。
Ａ：トンネル粉塵無添加・被験試料無添加での補正値
Ｂ：トンネル粉塵添加・被験試料無添加での補正値
Ｃ：トンネル粉塵添加・被験試料添加での補正値
結果を表５に示す。
MMP-9 mRNA expression increase suppression rate (%) = {(AB)-(AC)}/(AB)×100
Each term in the formula represents the following.
A: Correction value without addition of tunnel dust and test sample B: Correction value with addition of tunnel dust and no test sample C: Correction value with addition of tunnel dust and test sample The results are shown in Table 5.
表５に示すように、スターフルーツ果実抽出物（試料１）は、環境汚染物質によるＭＭＰ－９の発現上昇抑制作用として優れた作用を有することが確認された。 As shown in Table 5, the star fruit fruit extract (sample 1) was confirmed to have an excellent effect of suppressing the increase in MMP-9 expression caused by environmental pollutants.
〔配合例１〕
下記組成に従い、乳液を常法により製造した。
スターフルーツ果実抽出物 ０．０１ｇ
ホホバオイル ４．００ｇ
１，３－ブチレングリコール ３．００ｇ
アルブチン ３．００ｇ
ポリオキシエチレンセチルエーテル（20E.O.） ２．５０ｇ
オリーブオイル ２．００ｇ
スクワラン ２．００ｇ
セタノール ２．００ｇ
モノステアリン酸グリセリル ２．００ｇ
オレイン酸ポリオキシエチレンソルビタン（20E.O.） ２．００ｇ
パラオキシ安息香酸メチル ０．１５ｇ
グリチルレチン酸ステアリル ０．１０ｇ
黄杞エキス ０．１０ｇ
グリチルリチン酸ジカリウム ０．１０ｇ
イチョウ葉エキス ０．１０ｇ
コンキオリン ０．１０ｇ
オウバクエキス ０．１０ｇ
カミツレエキス ０．１０ｇ
香料 ０．０５ｇ
精製水 残部（全量を１００ｇとする）
[Formulation example 1]
A milky lotion was produced by a conventional method according to the following composition.
Starfruit fruit extract 0.01g
Jojoba oil 4.00g
1,3-butylene glycol 3.00g
Arbutin 3.00g
Polyoxyethylene cetyl ether (20E.O.) 2.50g
Olive oil 2.00g
Squalane 2.00g
Setanol 2.00g
Glyceryl monostearate 2.00g
Polyoxyethylene sorbitan oleate (20E.O.) 2.00g
Methyl paraoxybenzoate 0.15g
Stearyl glycyrrhetinate 0.10g
Huangji extract 0.10g
Dipotassium glycyrrhizinate 0.10g
Ginkgo biloba extract 0.10g
Conchiolin 0.10g
Aurora extract 0.10g
Chamomile extract 0.10g
Fragrance 0.05g
Remaining purified water (total amount is 100g)
〔配合例２〕
下記組成のクリームを常法により製造した。
スターフルーツ果実抽出物 ０．０５ｇ
クジンエキス ０．１ｇ
オウゴンエキス ０．１ｇ
流動パラフィン ５．０ｇ
サラシミツロウ ４．０ｇ
スクワラン １０．０ｇ
セタノール ３．０ｇ
ラノリン ２．０ｇ
ステアリン酸 １．０ｇ
オレイン酸ポリオキシエチレンソルビタン（20E.O.） １．５ｇ
モノステアリン酸グリセリル ３．０ｇ
油溶性甘草エキス ０．１ｇ
１，３－ブチレングリコール ６．０ｇ
パラオキシ安息香酸メチル １．５ｇ
香料 ０．１ｇ
精製水 残部（全量を１００ｇとする）
[Formulation example 2]
A cream having the following composition was produced by a conventional method.
Star fruit fruit extract 0.05g
Kujin extract 0.1g
Scutellariae extract 0.1g
Liquid paraffin 5.0g
White beeswax 4.0g
Squalane 10.0g
Setanol 3.0g
Lanolin 2.0g
Stearic acid 1.0g
Polyoxyethylene sorbitan oleate (20E.O.) 1.5g
Glyceryl monostearate 3.0g
Oil-soluble licorice extract 0.1g
1,3-butylene glycol 6.0g
Methyl paraoxybenzoate 1.5g
Fragrance 0.1g
Remaining purified water (total amount is 100g)
〔配合例３〕
下記組成の美容液を常法により製造した。
スターフルーツ果実抽出物 ０．０１ｇ
カミツレエキス ０．１ｇ
ニンジンエキス ０．１ｇ
キサンタンガム ０．３ｇ
ヒドロキシエチルセルロース ０．１ｇ
カルボキシビニルポリマー ０．１ｇ
１，３－ブチレングリコール ４．０ｇ
グリチルリチン酸ジカリウム ０．１ｇ
グリセリン ２．０ｇ
水酸化カリウム ０．２５ｇ
香料 ０．０１ｇ
防腐剤（パラオキシ安息香酸メチル） ０．１５ｇ
エタノール ２．０ｇ
精製水 残部（全量を１００ｇとする）
[Formulation example 3]
A beauty serum having the following composition was produced by a conventional method.
Starfruit fruit extract 0.01g
Chamomile extract 0.1g
Carrot extract 0.1g
Xanthan gum 0.3g
Hydroxyethylcellulose 0.1g
Carboxyvinyl polymer 0.1g
1,3-butylene glycol 4.0g
Dipotassium glycyrrhizinate 0.1g
Glycerin 2.0g
Potassium hydroxide 0.25g
Fragrance 0.01g
Preservative (methyl paraoxybenzoate) 0.15g
Ethanol 2.0g
Remaining purified water (total amount is 100g)
〔配合例４〕
下記組成のヘアートニックを常法により製造した。
スターフルーツ果実抽出物 ０．５ｇ
塩酸ピリドキシン ０．１ｇ
レゾルシン ０．０１ｇ
Ｄ－パントテニルアルコール ０．１ｇ
グリチルリチン酸ジカリウム ０．１ｇ
Ｌ－メントール ０．０５ｇ
１，３－ブチレングリコール ４．０ｇ
ニンジンエキス ０．２ｇ
エタノール ２５．０ｇ
香料 適量
精製水 残部（全量を１００ｇとする）
[Formulation example 4]
A hair tonic having the following composition was produced by a conventional method.
Star fruit fruit extract 0.5g
Pyridoxine hydrochloride 0.1g
Resorcinol 0.01g
D-pantothenyl alcohol 0.1g
Dipotassium glycyrrhizinate 0.1g
L-menthol 0.05g
1,3-butylene glycol 4.0g
Carrot extract 0.2g
Ethanol 25.0g
Flavoring appropriate amount Purified water Remainder (total amount is 100g)
〔配合例５〕
常法により、以下の組成を有する錠剤を製造した。
スターフルーツ果実抽出物 ５．０ｍｇ
ドロマイト（カルシウム20％、マグネシウム10％含有） ８３．４ｍｇ
カゼインホスホペプチド １６．７ｍｇ
ビタミンＣ ３３．４ｍｇ
マルチトール １３６．８ｍｇ
コラーゲン １２．７ｍｇ
ショ糖脂肪酸エステル １２．０ｍｇ
[Formulation example 5]
Tablets having the following composition were manufactured by a conventional method.
Starfruit fruit extract 5.0mg
Dolomite (contains 20% calcium and 10% magnesium) 83.4mg
Casein phosphopeptide 16.7mg
Vitamin C 33.4mg
Maltitol 136.8mg
Collagen 12.7mg
Sucrose fatty acid ester 12.0mg
〔配合例６〕
常法により、以下の組成を有する経口液状製剤を製造した。
＜１アンプル（１本１００ｍＬ）中の組成＞
スターフルーツ果実抽出物 ０．３質量％
ソルビット １２．０質量％
安息香酸ナトリウム ０．１質量％
香料 １．０質量％
硫酸カルシウム ０．５質量％
精製水 残部（１００質量％）
[Blend example 6]
An oral liquid preparation having the following composition was produced by a conventional method.
<Composition in 1 ampoule (100 mL per bottle)>
Star fruit fruit extract 0.3% by mass
Sorvit 12.0% by mass
Sodium benzoate 0.1% by mass
Fragrance 1.0% by mass
Calcium sulfate 0.5% by mass
Purified water remainder (100% by mass)
本発明に係る、環境汚染物質によるダメージ抑制剤、環境汚染物質によるダメージ抑制用化粧料及び環境汚染物質によるダメージ抑制用飲食品は、環境汚染物質による様々な症状（例えば、気管支炎、喘息、発癌、心疾患、骨や軟骨の破壊、発熱、皮膚の保水力低下、皮膚のバリア機能低下、肌荒れ、炎症及び毛髪の損傷等）の予防、治療又は改善に大きく貢献できる。 The agent for suppressing damage caused by environmental pollutants, the cosmetics for suppressing damage caused by environmental pollutants, and the food and drink products for suppressing damage caused by environmental pollutants according to the present invention can be used to treat various symptoms caused by environmental pollutants (for example, bronchitis, asthma, carcinogenesis, etc.). , heart disease, bone and cartilage destruction, fever, decreased skin water retention capacity, decreased skin barrier function, rough skin, inflammation, hair damage, etc.).
Claims (4)
大気汚染物質によるタンパク質カルボニル化を抑制する用途及び／又は大気汚染物質による炎症関連遺伝子の発現上昇を抑制する用途に用いられる
ことを特徴とする、大気汚染物質によるダメージ抑制剤。 The active ingredient is extract from star fruit (Averrhoa carambola L.) .
Used for suppressing protein carbonylation caused by air pollutants and/or suppressing increased expression of inflammation-related genes caused by air pollutants.
An agent for suppressing damage caused by air pollutants.
大気汚染物質によるタンパク質カルボニル化を抑制する用途及び／又は大気汚染物質による炎症関連遺伝子の発現上昇を抑制する用途に用いられる
ことを特徴とする、大気汚染物質によるダメージ抑制用化粧料。 Contains extract from star fruit (Averrhoa carambola L.) ,
Used for suppressing protein carbonylation caused by air pollutants and/or suppressing increased expression of inflammation-related genes caused by air pollutants.
A cosmetic for suppressing damage caused by air pollutants.
大気汚染物質によるタンパク質カルボニル化を抑制する用途及び／又は大気汚染物質による炎症関連遺伝子の発現上昇を抑制する用途に用いられる
ことを特徴とする、大気汚染物質によるダメージ抑制用飲食品。 Contains extract from star fruit (Averrhoa carambola L.) ,
Used for suppressing protein carbonylation caused by air pollutants and/or suppressing increased expression of inflammation-related genes caused by air pollutants.
A food and drink product for suppressing damage caused by air pollutants.
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| JP2019190185A JP7455350B2 (en) | 2019-10-17 | 2019-10-17 | Damage inhibitors, cosmetics, and food and beverages caused by air pollutants |
| CN202011098078.3A CN112691128A (en) | 2019-10-17 | 2020-10-14 | Inhibitor for damage caused by environmental pollutants, cosmetic and food and drink |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003300893A (en) | 2002-04-09 | 2003-10-21 | Maruzen Pharmaceut Co Ltd | Skin cosmetic and food and drink for beauty |
| US20090170788A1 (en) | 2006-01-16 | 2009-07-02 | Dong-Heon Shin | Novel use of 1, 2, 3, 4, 6-penta-o-galloyl-beta-d-glucose |
| JP2018150262A (en) | 2017-03-10 | 2018-09-27 | 丸善製薬株式会社 | Anti-aging agent |
| JP2018203725A (en) | 2017-05-30 | 2018-12-27 | 東亜化成株式会社 | Skin darkening inhibitor, cosmetics and skin external preparation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103393577B (en) * | 2013-08-07 | 2016-01-20 | 伽蓝（集团）股份有限公司 | A kind of Dermatologic preparation composition containing fruit extract mixture |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003300893A (en) | 2002-04-09 | 2003-10-21 | Maruzen Pharmaceut Co Ltd | Skin cosmetic and food and drink for beauty |
| US20090170788A1 (en) | 2006-01-16 | 2009-07-02 | Dong-Heon Shin | Novel use of 1, 2, 3, 4, 6-penta-o-galloyl-beta-d-glucose |
| JP2018150262A (en) | 2017-03-10 | 2018-09-27 | 丸善製薬株式会社 | Anti-aging agent |
| JP2018203725A (en) | 2017-05-30 | 2018-12-27 | 東亜化成株式会社 | Skin darkening inhibitor, cosmetics and skin external preparation |
Non-Patent Citations (3)
| Title |
|---|
| FEBS J.，2019年02月，Vol.286 No.3，p.459-478 |
| Inflamm. Regen，2019年06月，Vol.39 Article Number:12，p.1-16 |
| Sci. Rep.，2017年，Vol.7 Article Number:6704，p.1-12 |
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