CN103099967A - Dendrobe aqueous extract and application thereof - Google Patents
Dendrobe aqueous extract and application thereof Download PDFInfo
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- CN103099967A CN103099967A CN2013100460706A CN201310046070A CN103099967A CN 103099967 A CN103099967 A CN 103099967A CN 2013100460706 A CN2013100460706 A CN 2013100460706A CN 201310046070 A CN201310046070 A CN 201310046070A CN 103099967 A CN103099967 A CN 103099967A
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Abstract
The invention relates to dendrobe aqueous extract and application thereof, and belongs to the technical field of pharmacy. The dendrobe aqueous extract disclosed by the invention is obtained by the following method: a, cutting dendrobe into pieces by scissors and adding the dendrobe pieces to water in the proportion W/V of 1:(10-20) for boiling; continuously keeping the boiling state for 2 hours to 4 hours after the drendrobe is boiled in the water; removing the residues by filtering through gauze and collecting the water extract; b, repeating the step a for twice to four times, combining the extracting solution every time; and freezing and drying the extracting solutions to obtain powder dendrobe water extract. The dendrobe is wild or artificially-cultivated fresh dendrobe or dried dendrobe. The invention further relates to the application of the dendrobe aqueous extract in preparing hemostatics. According to the dendrobe aqueous extract and the application thereof disclosed by the invention, the dendrobe aqueous extract provided by the invention has strong pharmacological action, the bleeding time is remarkably shortened and the medical prospect of the dendrobe aqueous extract in developing and preparing hemostatics are disclosed; and secondly, a novel application field of the dendrobe is developed.
Description
Technical field
The present invention relates to a kind of Herba Dendrobii water extract and application thereof, belong to the pharmaceutical technology field.
Background technology
Blood is the important substance that sustains life, and contains various nutritional labelings, as inorganic salt, oxygen and products of cellular metabolism, hormone, enzyme and antibody etc., has the effect of regulating organ activity and defence harmful substance.Adult's blood accounts for 8% of own wt, and approximately per kilogram of body weight has 60-80 ml blood.Human body occurs slowly a small amount of hemorrhage, can be by vasoconstriction, and the mechanism of action such as platelet aggregation formation blood clot reach haemostatic effect.But blood pressure drops will appear in hemorrhage more than 20% of total blood volume that reaches of human body, the serious symptoms such as shock, and the hemorrhage 40%-50% that surpasses total blood volume will be in peril of one's life.
Hemorrhage impact on body is also depended on hemorrhage type, hemorrhage speed and bleeding part except amount of bleeding.If the hemorrhage process of rhexis hemorrhage is rapid, when losing at short notice circulation volume 20%-25%, hemorrhagic shock can occur.Spilling property is hemorrhage, if hemorrhage when extensive, as hemorrhage because of portal hypertension generation popularity gastrointestinal tract mucosa in liver cirrhosis, also can cause hemorrhagic shock.Occur in the hemorrhage of vitals, even amount of bleeding is few, also can cause serious consequence, breaking as heart causes hematocele in pericardium, due to pericardial tamponade, can cause acute cardiac insufficiency.Therefore, in time and effectively use hemorrhage, can avoid the blood consume, prevent because severe loss of blood causes circulatory failure, to reach redemption life, ensure the purpose of people ' s health, therefore the application of hemorrhage is no matter in general treatment, wound or war wound rescue, all have great importance.
The onset of tradition haemostatic medicament is slower, and effect is undesirable when acute hemorrhage.Reptilase is the intravenous injection hemorrhage that goes on the market in recent years, to separate the enzyme preparation that obtains from snake venom, because it has quick-acting, efficient, convenient and is not subjected to the plurality of advantages such as the blood plasma thrombin inhibitor affects, thereby be used widely in surgical hemostasis and the treatment of various acute hemorrhage.But reptilase separates from snake venom and obtains, and there is certain difficulty in raw material sources, and are expensive, and owing to being macromolecular substances, also have the problems such as allergenicity.Therefore, be badly in need of at present safe and effective hemostasis natural drug.
Dendrobium (Dendrobium) is the first genus of the orchid family, comprises approximately 1500 kind of plant, mainly is distributed in Tropical Asia and Pacific Islands.China is 63 kinds of Dendrobium Sws approximately, are made at present 39 kinds of medicinal Dendrobium Sws, mainly are distributed in the ground such as Yunnan, Guangxi, Guangdong, Guizhou.Herba Dendrobii begins to be stated from Shennong's Herbal, has won fame both at home and abroad with the reputation of " a thousand pieces of gold grass ", is called " medicine circle giant panda " by international medicinal plants circle, is called by Taoist school first of " China's nine large Herba mesonae chinensis ".According to one's analysis, Herba Dendrobii contains multiple alkaloid, dendrobium polysaccharide and trace element and aminoacid etc., has reinforcing stomach reg fluid, the effects such as nourishing YIN and clearing away heat, the sharp throat that makes eye bright.To pharmacological research and the application of Herba Dendrobii, except concentrating on gastrointestinal effect research, Chinese scholars finds that also Herba Dendrobii has enhancing human body immunity power, antitumor, treatment cataract, the multiple efficacies such as blood sugar lowering in recent years.
But up to now, there is not yet the research report that Herba Dendrobii and preparation thereof are used for the hemostasis aspect.
Summary of the invention
The object of the present invention is to provide a kind of Herba Dendrobii water extract and application thereof.
Purpose of the present invention is achieved by following technical proposals:
(1) basic ideas of technical solution of the present invention
1. the pharmacological action of drug hemostasis is broadly divided into: ⑴ shrinks local vascular; ⑵ act on coagulation process, shortens clotting time; ⑶ improve the angiopathy function, increases blood capillary to the resistance of damage; ⑷ suppress the activity of fibrinolysin.The method that multiple screening haemostatic medicament is arranged according to the mechanism of action of its pharmacology, such as: the mensuration in bleeding time, the mensuration of thrombin source and thrombin movable enzyme time, fibrinolysis functional examination, and the method for platelet inspection.But the mensuration in bleeding time is to observe tested material anastalsis to be had or not the experiment that affects and need primarily carry out.
2. the bleeding time refers to blood breakthrough blood capillary, and autohemorrhage is to naturally stopping blooding the needed time.In a single day blood capillary is cut off, body self can occur stress hemostasis reaction, the length in its bleeding time is main relevant with the blood capillary function with platelet, comprise that specifically blood capillary shrinks, the formation of hematoblastic adhesion, gathering and release and platelet thrombus, in case the function of platelet and blood capillary has occured extremely, can cause the generation of corresponding disease and follow the prolongation in bleeding time, such as the hereditary hemorrhagic telangiectasia that causes because of blood vessel structure or dysfunction; Platelet counts obviously minimizing causes constitutional or secondary thrombocytopenic purpura; The platelet adhesion reaction dysfunction causes angiohemophilia; Platelet aggregation causes thrombocytasthenia and hypofibrinogenemia etc. extremely.Therefore, change by giving to observe after the laboratory animal Herba Dendrobii extract its bleeding time, can confirm the hemostasia effect of Herba Dendrobii, Herba Dendrobii has important application prospect to the disease treatment that causes because of platelet and blood capillary dysfunction simultaneously.
(2) in order to understand better essence of the present invention, the below will illustrate its application in the preparation haemostatic medicament with pharmacological testing and the result of Herba Dendrobii.
Herba Dendrobii water extract of the present invention obtains through following method:
A. the ratio that is 1:10～20 in W/V after with shears, Herba Dendrobii being shredded is added to the water hots plate, and continues to keep 2～4 h after Herba Dendrobii boils in water, then discards residue with filtered through gauze, collects dendrobium extract;
B. repeat a step 2～4 time, merge each dendrobium extract, extracting solution obtains powdery Herba Dendrobii water extract after conventional lyophilization.
Described Herba Dendrobii refers to wild or tame fresh Herba Dendrobii or drying Herba Dendrobii that market is bought.
The application of Herba Dendrobii water extract of the present invention in the preparation haemostatic medicament.
Herba Dendrobii of the present invention also can be prepared into by conventional method the preparation of regular dosage form.
2. the hemostasia effect of Herba Dendrobii of the present invention and preparation thereof
(1) measuring principle in bleeding time
Under certain condition, after artificial prick skin blood capillary, naturally outpour nature from blood and stop the required time, be called bleeding time.The length in its bleeding time mainly is subjected to the impact of hematoblastic quality and quantity, capillary structure and function and platelet and blood capillary interaction, and it is less affected by blooc coagulation factor content and active function.After the docking of normal employing mice, the hemorrhage time that naturally stops is as the index in test bleeding time.
(2) assay method in bleeding time
After giving mice with Herba Dendrobii by the doses gavage, with the fixing mice of special holder, with milimeter scale measure Mus tail length and labelling it, 30～40 min(drug administration by injection 10 min) after, cut Mouse Tail-tip 0.3 cm place is cross-section or mouse tail far-end 1/3 place is cross-section with profit, treat that blood overflows the beginning timing automatically, drew drop of blood 1 time every 30 seconds with filter paper, until till blood stops when inhaling (filter paper depletion of blood) naturally, record the bleeding time, the data obtained and matched group carry out the significant difference analysis.
(3) result of the test of bleeding time
Result of the test shows, Herba Dendrobii has good hemostasia effect, can shorten the mouse tail vein bleeding time to reach more than 50%, can be as haemostatic medicament or drug regimen composition.
The present invention compared with prior art has following beneficial effect:
1. Herba Dendrobii pharmacological action provided by the invention is very strong, can obviously shorten the bleeding time, discloses its prospect in medicine in exploitation preparation haemostatic medicament.
2. the present invention is that Herba Dendrobii has been opened up a new application.
Description of drawings
Fig. 1 is that the Herba Dendrobii water extract can shorten the hemorrhage time of mouse tail vein significantly.
The specific embodiment
By specific embodiment given below and typical Application Example, further describe the present invention.
Device therefor of the present invention is existing conventional equipment.
Embodiment 1: the preparation of Herba Dendrobii water extract
Herba Dendrobii water extract of the present invention obtains through following method:
A. the ratio that is 1:10～20 in W/V after with shears, Herba Dendrobii being shredded is added to the water hots plate, and continues to keep 2～4 h after Herba Dendrobii boils in water, then discards residue with filtered through gauze, collects dendrobium extract;
B. repeat a step 2～4 time, merge each dendrobium extract, extracting solution obtains powdery Herba Dendrobii water extract after conventional lyophilization.
Described Herba Dendrobii refers to wild or tame fresh Herba Dendrobii or drying Herba Dendrobii that market is bought.
Embodiment 2: the anastalsis of Herba Dendrobii water extract
The present embodiment water extract used is the prepared Herba Dendrobii water extract of embodiment 1.
Choose the C57BL/6 Strains of Mouse, body weight 20-25g, male.Be divided at random three groups, be respectively matched group (0 g/kg), Herba Dendrobii water extract low dose group (0.5 g/kg), Herba Dendrobii water extract high dose group (1.0 g/kg), 9 every group, after administration, 0.5 h measures the bleeding time of mouse tail vein.Medication is matched group gavage tap water, and the Herba Dendrobii water extract is low, the Herba Dendrobii water extract solution of high dose group difference gavage various dose.Herba Dendrobii water extract solution adopts the prepared Herba Dendrobii water extract of embodiment 1 to prepare.During experiment, mice is put into special holder, then cross-section in order to cutting Mouse Tail-tip 0.3 cm place, treat that blood overflows the beginning timing automatically, drew drop of blood 1 time every 30 seconds with filter paper, until till blood stops when inhaling (filter paper depletion of blood) naturally, record the bleeding time.The results are shown in Table 1 and accompanying drawing 1.
The impact of table 1 Herba Dendrobii water extract on the mouse tail vein blood bleeding time
Annotate: result represents with " mean+SD "; Administration group and matched group are relatively processed through the T inspection statistics, and n.s. is for remarkable,
*P ﹤ 0.05,
*P ﹤ 0.01.
Epidemiological Analysis by statistics, after gastric infusion 30 min, the hemorrhage time of mouse tail vein of matched group, Herba Dendrobii water extract low dose group and Herba Dendrobii water extract high dose group is respectively 40.67 ± 5.13 min, 13.83 ± 1.59 min, 17.33 ± 0.88 min.Herba Dendrobii water extract low dose group is compared the shortening mouse tail vein with matched group with high dose group bleeding time reaches more than 50%, is respectively 65.99% and 57.39%.Herba Dendrobii water extract low dose group and matched group relatively have utmost point significant difference (P＜0.01), and Herba Dendrobii water extract high dose group and matched group relatively have significant difference (P＜0.05); And low dose group is compared bleeding time difference not significantly (P〉0.05) with high dose group, and the result shows: the Herba Dendrobii water extract has the hemostasia effect of highly significant to mice.
Claims (3)
1. Herba Dendrobii water extract is characterized in that this Herba Dendrobii water extract obtains through following method:
A. the ratio that is 1:10～20 in W/V after with shears, Herba Dendrobii being shredded is added to the water hots plate, and continues to keep 2～4h after Herba Dendrobii boils in water, then discards residue with filtered through gauze, collects water extraction liquid;
B. repeat a step 2～4 time, merge each extracting solution, extracting solution obtains powdery Herba Dendrobii water extract after lyophilization.
2. Herba Dendrobii water extract claimed in claim 1, is characterized in that described Herba Dendrobii refers to wild or tame fresh Herba Dendrobii or drying Herba Dendrobii.
3. claim 1 and the 2 described Herba Dendrobii water extracts application in the preparation haemostatic medicament.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103735858A (en) * | 2013-12-18 | 2014-04-23 | 广西健宝石斛有限责任公司 | Application of Dendrobium officinale fruit extract in preparing medicines for treating upper gastrointestinal bleeding |
| CN103735860A (en) * | 2013-12-18 | 2014-04-23 | 广西健宝石斛有限责任公司 | Application of Dendrobium officinale fruit in preparing medicines for treating external hemorrhage |
| CN103751555A (en) * | 2013-12-18 | 2014-04-30 | 广西健宝石斛有限责任公司 | Dendrobium officinale patch with hemostatic function |
| CN103977266A (en) * | 2014-04-14 | 2014-08-13 | 广西健宝石斛有限责任公司 | Dendrobium officinale traditional Chinese medicine composition for hemostasis |
| CN105012708A (en) * | 2015-06-30 | 2015-11-04 | 广西健宝石斛有限责任公司 | Dendrobium candidum Wall.ex Lindl. aerosol |
| CN105327148A (en) * | 2015-12-11 | 2016-02-17 | 杭州华都医药有限公司 | Method for preparing dendrobe cream |
| CN105901733A (en) * | 2016-02-22 | 2016-08-31 | 上海交通大学 | Antioxidation application of dendrobe leaf |
| CN112107651A (en) * | 2020-10-20 | 2020-12-22 | 云南农业大学 | Traditional Chinese medicine compound for promoting diabetic wound healing |
| CN113940976A (en) * | 2021-10-30 | 2022-01-18 | 安徽同济生生物科技有限公司 | Preparation method of fresh dendrobium aqueous extract for treating cell injury and inflammation |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103735858A (en) * | 2013-12-18 | 2014-04-23 | 广西健宝石斛有限责任公司 | Application of Dendrobium officinale fruit extract in preparing medicines for treating upper gastrointestinal bleeding |
| CN103735860A (en) * | 2013-12-18 | 2014-04-23 | 广西健宝石斛有限责任公司 | Application of Dendrobium officinale fruit in preparing medicines for treating external hemorrhage |
| CN103751555A (en) * | 2013-12-18 | 2014-04-30 | 广西健宝石斛有限责任公司 | Dendrobium officinale patch with hemostatic function |
| CN103977266A (en) * | 2014-04-14 | 2014-08-13 | 广西健宝石斛有限责任公司 | Dendrobium officinale traditional Chinese medicine composition for hemostasis |
| CN103977266B (en) * | 2014-04-14 | 2017-05-24 | 广西健宝石斛有限责任公司 | Dendrobium officinale traditional Chinese medicine composition for hemostasis |
| CN105012708A (en) * | 2015-06-30 | 2015-11-04 | 广西健宝石斛有限责任公司 | Dendrobium candidum Wall.ex Lindl. aerosol |
| CN105327148A (en) * | 2015-12-11 | 2016-02-17 | 杭州华都医药有限公司 | Method for preparing dendrobe cream |
| CN105327148B (en) * | 2015-12-11 | 2019-02-01 | 杭州华都医药有限公司 | The preparation method of dendrobium nobile cream |
| CN105901733A (en) * | 2016-02-22 | 2016-08-31 | 上海交通大学 | Antioxidation application of dendrobe leaf |
| CN105901733B (en) * | 2016-02-22 | 2020-06-09 | 上海交通大学 | Antioxidation application of dendrobe leaves |
| CN112107651A (en) * | 2020-10-20 | 2020-12-22 | 云南农业大学 | Traditional Chinese medicine compound for promoting diabetic wound healing |
| CN113940976A (en) * | 2021-10-30 | 2022-01-18 | 安徽同济生生物科技有限公司 | Preparation method of fresh dendrobium aqueous extract for treating cell injury and inflammation |
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Inventor after: Tian Yang Inventor after: Sheng Jun Inventor after: Bai Zhongbin Inventor after: Wang Xuanjun Inventor after: Hao Shumei Inventor after: Gao Xiaoyu Inventor after: Shi Chongying Inventor after: Fang Chongye Inventor before: Tian Yang Inventor before: Sheng Jun Inventor before: Bai Zhongbin Inventor before: Hao Shumei Inventor before: Gao Xiaoyu Inventor before: Shi Chongying Inventor before: Fang Chongye |
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Free format text: CORRECT: INVENTOR; FROM: TIAN YANG SHENG JUN BAI ZHONGBIN HAO SHUMEI GAO XIAOYU SHI CHONGYING FANG CHONGYE TO: TIAN YANG SHENG JUN BAI ZHONGBIN WANG XUANJUN HAO SHUMEI GAO XIAOYU SHI CHONGYING FANG CHONGYE |
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Application publication date: 20130515 |