CN100497396C - Dendrobium candidum polysaccharide extractive, medicine composition thereof and its preparation and use - Google Patents
Dendrobium candidum polysaccharide extractive, medicine composition thereof and its preparation and use Download PDFInfo
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- CN100497396C CN100497396C CNB200610145677XA CN200610145677A CN100497396C CN 100497396 C CN100497396 C CN 100497396C CN B200610145677X A CNB200610145677X A CN B200610145677XA CN 200610145677 A CN200610145677 A CN 200610145677A CN 100497396 C CN100497396 C CN 100497396C
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Abstract
This invention relates to polysaccharides extract from Dendrobium candidum and its preparation method. The molecular weight of the polysaccharide extract of Dendrobium candidum is 10000-300000. The content of polysaccharide is no less than 25 wt%. The invention also relates to a Chinese medicinal composition containing polysaccharide extract from Dendrobium candidum and its preparation method, and application of the polysaccharide extract in preparing medicine for auxiliary treatment of diseases caused by hypoimmunity. The extract and Chinese medicinal composition containing the same can improve immunologic function with high bioavailability, no toxicity and adverse side effect. the preparation method is simple and convenient for operation and commercial process.
Description
Technical field
The present invention relates to a kind of Chinese medical extract, be specifically related to a kind of dendrobium candidum polysaccharide extractive and its preparation method and application thereof.
The invention still further relates to a kind of pharmaceutical composition, be specifically related to a kind of pharmaceutical composition that contains described dendrobium candidum polysaccharide extractive and preparation method thereof.
Technical background
Herba Dendrobii (Dendrobium candidum wall.ex lindl.) is Dendrobium plant (the orchid family), record in 2000 editions one one of the Pharmacopoeia of the People's Republic of China, it is the important traditional Chinese medicine material of China, pharmaceutical use was praised highly by the successive dynasties, and Herba Dendrobii is product on the stem of noble dendrobium, because of its special living environment and distinguished nourishing effects rank first of " Chinese nine immortal grass ".The main component of Herba Dendrobii is polyose and alkaloid, studies show that the drug effect of polysaccharide component and Herba Dendrobii as the immunological competence that strengthen to improve body, antitumor, anti-ageing, antifatigue, hypoglycemic etc., has confidential relation.The Herba Dendrobii product is the compound preparation of Herba Dendrobii in the market, and the Herba Dendrobii extraction process is simple, some is then simply beaten powder and is used as medicine, and these all exist, and action component is indeterminate, quality is uncontrollable, bioavailability is low, be difficult for by drawbacks such as human body absorb.In patent 200410066941.1, the extraction process of Herba Dendrobii effective constituent only is simple water extract-alcohol precipitation also among the 200310113916.X, does not meet the requirement of the modernization of Chinese medicine, and the relevant quality control standard of the no iron sheet stem of noble dendrobium in the preparation.Do not see on the market at present that medicine or pharmaceutical composition relevant for Dendrobium officinale polysaccharide occur yet.
Summary of the invention
In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a kind of bioavailability height, the dendrobium candidum polysaccharide extractive that has the enhancing body immunologic function, keeps fit anti-ageing effect.
Another object of the present invention provides a kind of preparation method of above-mentioned dendrobium candidum polysaccharide extractive.
A further object of the present invention provides a kind of pharmaceutical composition that contains above-mentioned dendrobium candidum polysaccharide extractive.
The present invention also provides a kind of above-mentioned preparation of drug combination method that contains dendrobium candidum polysaccharide extractive.
The present invention and then the application of above-mentioned dendrobium candidum polysaccharide extractive in the medicine of the disease that preparation assisting therapy hypoimmunity causes is provided.
Dendrobium candidum polysaccharide extractive of the present invention is that raw material extracts with the Herba Dendrobii, and its molecular weight is 1-30 ten thousand, and polysaccharide content is not less than 25% (weight).And its polysaccharide molecular weight preferably 10～300,000, its polysaccharide content is preferably 25%～50% (weight).
The present invention also provides a kind of preparation method of dendrobium candidum polysaccharide extractive, and it may further comprise the steps:
(1) degreasing: after Herba Dendrobii is ground into meal,, make the dregs of a decoction with 10-30 times of organic solvent backflow 1-4 time;
(2) extract: the above-mentioned dregs of a decoction of boiling 1-4 time, each 1-4 hour, add water 10-30 doubly at every turn, obtain decoction liquor;
(3) concentrate: merge above-mentioned decoction liquor, be concentrated to every ml and be equivalent to contain crude drug 0.2-2g, obtain concentrated solution;
(4) alcohol precipitation: in above-mentioned concentrated solution, add an amount of ethanol and precipitate, make ethanol content reach 50%-80% (volume percent), with pure liquid standing over night;
(5) drying: above-mentioned pure liquid is filtered, after the gained precipitation adds 2-3 times of organic solvent washing, drain, drying under reduced pressure obtains the dendrobium candidum polysaccharide extractive that polysaccharide content is not less than 25% (weight).
Wherein, the organic solvent in the described step (1) is the ethanol water mixture of sherwood oil, ethyl acetate or 80%-95%
Extraction temperature in the described step (2) is 50-100 ℃, and preferred temperature is at 80～100 ℃.
Organic solvent in the described step (5) is sherwood oil, acetone, dehydrated alcohol, methyl alcohol, ether, chloroform or its mixture, preferred dehydrated alcohol.
Described preparation method can also be further purified, and concrete purification step is as follows:
(1) dissolving: the dendrobium candidum polysaccharide extractive that aforesaid method is obtained adds 20-100 times of water, and heated and stirred is fully dissolved it, and the gained liquid at high speed is centrifugal, gets supernatant liquor;
(2) deproteinated: with above-mentioned supernatant liquor, be diluted with water to 0.01-0.05g/ml, add 1/5 volume sevage reagent (chloroform: the extraction of propyl carbinol=4:1) 1-4 time, the centrifugal precipitation of removing must supernatant liquor;
(3) ultrafiltration: the ultra-filtration membrane that is 10K with above-mentioned supernatant liquor molecular weight concentrates, and obtains concentrated solution;
(4) column chromatography: with above-mentioned concentrated solution cross DEAE-52 (DEAE: resin column DEAE), with anthrone sulfuric acid process online detection, and use the eluent wash-out successively.Wherein can collect first-class fluid (that is: dendrobium candidum polysaccharide extractive A) and second effluent liquid (that is: dendrobium candidum polysaccharide extractive B) respectively;
(5) drying:, promptly obtain the Dendrobium officinale polysaccharide of purifying with freeze-drying behind the above-mentioned elutriant concentrating under reduced pressure.
Wherein, the elutriant in the described concentrated and purified step (4) is deionized water and salts solution, and described salts solution is preferably 0.05M-0.5M NaCl solution.
The above-mentioned polysaccharide that obtains, its assay adopts the anthrone sulfuric acid process, and the gained polysaccharide content is 25%-50% (weight).Described polysaccharide molecular weight adopts liquid phase efficient gel chromatogram osmose process to measure.
The present invention also provides a kind of pharmaceutical composition that contains dendrobium candidum polysaccharide extractive, what it comprised the treatment significant quantity is acceptable carrier on the dendrobium candidum polysaccharide extractive of raw material and the pharmacology with the Herba Dendrobii, the molecular weight of wherein said dendrobium candidum polysaccharide extractive is 1-30 ten thousand, and polysaccharide content is not less than 25% (weight) in described Herba Dendrobii extract.
Wherein said dendrobium candidum polysaccharide extractive can prepare by the preparation method of above-mentioned dendrobium candidum polysaccharide extractive of the present invention.
The described pharmaceutical composition that contains dendrobium candidum polysaccharide extractive, by described dendrobium candidum polysaccharide extractive as active ingredient, with medicinal significant quantity, add acceptable assistant agent on conventional pharmaceutical carrier and other conventional pharmaceutical again, for example: thinner, vehicle, weighting agent, tackiness agent, wetting agent, disintegrating agent, tensio-active agent and/or buffer reagent etc. are prepared into the pharmaceutical composition that is used for the disease that assisting therapy causes because of hypoimmunity.Wherein, described weighting agent such as starch, N.F,USP MANNITOL, dextrin, sucrose, lactose, Microcrystalline Cellulose etc.; Tackiness agent such as derivatived cellulose, alginate, gelatin, polyvinylpyrrolidone; Wetting agent such as glycerine, certain density ethanol; Disintegrating agent such as methyl starch sodium, hydroxylated cellulose, crosslinked Walocel MT 20.000PV, agar and sodium bicarbonate; Tensio-active agent such as talcum powder, calcium stearate, Magnesium Stearate, micropowder silica gel and polyoxyethylene glycol etc.Can also in described pharmaceutical composition, add other auxiliary materials in addition, as flavouring agent, sweeting agent etc.
The described pharmaceutical composition that contains dendrobium candidum polysaccharide extractive can be multiple formulation, and preferred dosage form is oral solid formulation, oral semi-solid preparation, oral liquid or injection.
Wherein, described oral solid formulation is granule, tablet, pill, pill, hard capsule, soft capsule, solid dispersion, powder, fine granule, pellet, microcapsule, microspheres agent or other pharmaceutically acceptable oral solid formulations; Described oral liquid is oral liquid or other pharmaceutically acceptable oral liquids; Described injection is injection liquid, freeze-drying preparation for injection, sterile packaged preparation for injection, sodium chloride injection, glucose injection or other pharmaceutically acceptable injections.
Preferably, described oral solid formulation is that granule, described oral liquid are oral liquid, and described injection is injection liquid or injectable lyophilised powder.
Pharmaceutical composition of the present invention can be applied to the patient who needs this curative effect by the mode of oral, rectum or administered parenterally, be used for when oral, conventional solid preparation such as tablet, capsule, pulvis, granule etc. be can be made into, other liquid preparations of liquid preparation such as water or oil-suspending agent such as syrup, mixture, elixir etc. made; When being used for administered parenterally, can be made into solution, powder pin, water or the oiliness suspension agent etc. of injection.The preferred form of the present invention is powder pin, injection and tablet, capsule, granule.
The present invention further provides a kind of preparation of drug combination method that contains dendrobium candidum polysaccharide extractive, it is by acceptable carrier on the dendrobium candidum polysaccharide extractive that forms by method for preparing or its purified extract and the pharmacology, is mixed with according to the ordinary method of this area to form.
The present invention also provides the application of a kind of dendrobium candidum polysaccharide extractive in the medicine of the disease that preparation assisting therapy hypoimmunity causes.The dendrobium candidum polysaccharide extractive that forms by method for preparing of the present invention also is used to prepare the multiple medicine that is used for the disease that assisting therapy causes because of hypoimmunity.
The inventor explores through a large amount of research, develops a kind of dendrobium candidum polysaccharide extractive and contains its pharmaceutical composition.Its preparation method is convenient to operation and suitability for industrialized production, and the dendrobium candidum polysaccharide extractive of preparing and contain its pharmaceutical composition bioavailability height, and remarkable enhancing body immunologic function has and keeps fit anti-ageing health-care effect.
Dendrobium candidum polysaccharide extractive of the present invention and pharmaceutical composition thereof, the water-soluble remarkable enhancing of the polysaccharide that contains is difficult for degraded, and is quality controllable, and preparation method's of the present invention whole process of production is simple to operate, carries out large-scale production easily, and cost is low.In addition, pharmaceutical composition of the present invention is used for the assisting therapy hypoimmunity and the curative effect of disease that causes is reliable, and almost has no adverse reaction.
Description of drawings
Fig. 1 is the separation-extraction technology schema of the Dendrobium officinale polysaccharide among the present invention.
Fig. 2 is the concentrated and purified process flow sheet of the Dendrobium officinale polysaccharide among the present invention.
Fig. 3 is the influence to CD4/CD8 of the Dendrobium officinale polysaccharide for preparing among the present invention.
Fig. 4 is the influence to the bone marrow cells in mice number of the Dendrobium officinale polysaccharide for preparing among the present invention.
Fig. 5 is the influence to mouse thymus of the Dendrobium officinale polysaccharide for preparing among the present invention.
Fig. 6 is the influence to the mouse body weight of the Dendrobium officinale polysaccharide for preparing among the present invention.
For proving its effect, the present inventor has studied its impact to the immunocompromised immune function of mice by dendrobium candidum polysaccharide extractive A and B that the method that provides among the embodiment 2 in the following specific embodiment prepares, and experimental result is as follows:
1, experiment material
Animal used as test: Kunming mouse, male, 18-20g, totally 100
Experimental drug: endoxan, Lentinan, Dendrobium officinale polysaccharide A, B
2, experiment grouping and step
Experiment mice is divided into 16 groups at random, 6 every group. The normal diet feeding, 5g/ pcs/day, altogether 14 days, each was organized except normal group, and the 7th, 9,11,13 day, every mouse lumbar injection every day 60mg/kg Endoxan. Normal group mouse then injecting normal saline is contrast.
(1) normal group: every day is with 0.5% carboxymethylcellulose sodium solution gavage, 0.1ml/10g, continuous irrigation Stomach 14 days.
(2) model group: 7th, 9,11,13 days, every mouse lumbar injection every day 60mg/ml ring phosphorus Acid amides, simultaneously every day is with 0.5% carboxymethylcellulose sodium solution gavage, 0.1ml/10g, continuously gavage 14 My god.
(3) Dendrobium officinale polysaccharide B is oral group:
Get Dendrobium officinale polysaccharide B and make the 0.5mg/ml oral administration solution, with the 0.1ml/10g gastric infusion.
(4) Dendrobium officinale polysaccharide B injection group:
Get Dendrobium officinale polysaccharide B and make the 0.1mg/ml injection solution, with the 0.1ml/10g intraperitoneal administration.
(5) Dendrobium officinale polysaccharide A is oral group:
Get Dendrobium officinale polysaccharide A and make the 0.5mg/ml oral administration solution, with the 0.1ml/10g gastric infusion.
(6) lentinan group (positive controls):
Get altogether a slice of Lentinan tablet (containing lentinan 10mg/ sheet), after milling, be dissolved in 20ml Contain in the distilled water of 0.5% sodium carboxymethylcellulose, make the 0.5mg/ml suspension, with 0.1ml/10g Administration.
3, experimental result
Dendrobium candidum polysaccharide extractive the results are shown in Table 1 to the impact of immune function of mice.
Table 1 dendrobium candidum polysaccharide extractive is to the impact of immune function of mice
(1) to the impact of CD4/CD8
The results are shown in Figure 3, show: Dendrobium officinale polysaccharide B oral administration and drug administration by injection all can obviously be carried The ratio of high mouse CD4/CD8. Compare with normal group and model group, Dendrobium officinale polysaccharide B is oral to be given Medicine and drug administration by injection all can obviously improve the ratio of mouse CD4/CD8; And more obvious than lentinan effect. Prompting Dendrobium officinale polysaccharide B, A have the effect that strengthens mouse immunity.
(2) to the inhibiting impact of bone marrow cell
The results are shown in Figure 4, show: compare with normal group and model group, Dendrobium officinale polysaccharide B is oral to be given Medicine and drug administration by injection, Dendrobium officinale polysaccharide A oral administration all can obviously resist the bone that endoxan causes The myelocyte inhibitory action. And more obvious than lentinan effect. Prompting Dendrobium officinale polysaccharide B has enhancing little The effect of mouse immunity.
(3) to the impact of thymus gland
The results are shown in Figure 5, show: Dendrobium officinale polysaccharide B injection and Dendrobium officinale polysaccharide A oral administration All can increase the thymic weight of mouse. Compare with normal group and model group, Dendrobium officinale polysaccharide A is oral Administration can obviously add the weight of thymus gland, and is also more apparent in view than its effect with the lentinan group; But iron sheet stone Dry measure used in former times Polysaccharide B oral administration is only more obvious than model group effect. Pointing out Dendrobium officinale polysaccharide B, A to have increases The effect of strong mouse immunity.
(4) impact of body weight
The results are shown in Figure 6, show: compare Dendrobium officinale polysaccharide B drug administration by injection, iron sheet with model group Dendrobium polysaccharide A oral administration can obviously increase the body weight of normal mouse and immunocompromised mouse; Iron sheet stone Dry measure used in former times Polysaccharide B oral administration is only obvious than model group effect. Prompting Dendrobium officinale polysaccharide B, A have enhancing The effect of mouse immunity.
Above experimental data and result can show that pharmaceutical composition of the present invention has remarkable enhancing immunity The function of power, and substantially have no side effect.
Embodiment
Below will the invention will be further described by embodiment, these descriptions are not to do further to limit to content of the present invention.One skilled in the art will understand that to be equal to replacement to what the technical characterictic of content of the present invention was done, or corresponding the improvement, still belong within protection scope of the present invention.
The preparation of embodiment 1 dendrobium candidum polysaccharide extractive
Get Herba Dendrobii 500g, after picking, be ground into meal, put in the container, add 10 times of amount 95% alcohol reflux 2 times, each 1 hour, filtrate recycling ethanol, the dregs of a decoction added 25 times of water gagings and extract 4 times in 80 ℃, each 2 hours, filtrate merged, and filtered, be evaporated to 1000ml, add 95% ethanol 3750ml, standing over night, filter, filtrate recycling ethanol, precipitation is measured absolute ethanol washings with 3 times, filter is done, and 50 ℃ of following vacuum-dryings get dendrobium candidum polysaccharide extractive.Recording its polysaccharide content is 44.18% (perhaps 441.8mg/g).
The preparation of embodiment 2 dendrobium candidum polysaccharide extractives
Get Herba Dendrobii 500g, after picking, be ground into meal, put in the container, add 15 times of amount 85% alcohol reflux 2 times, each 1 hour, filtrate recycling ethanol, the dregs of a decoction added 25 times of water gagings and extract 4 times in 100 ℃, each 2 hours, filtrate merged, and filtered, be evaporated to 1250ml, add 95% ethanol 4700ml, standing over night, filter, filtrate recycling ethanol, precipitation is measured dehydrated alcohols and washing with acetone with 2 times, filter is done, and 50 ℃ of following vacuum-dryings get dendrobium candidum polysaccharide extractive.Recording its polysaccharide content is 42.73% (perhaps 427.3mg/g).
The preparation of the dendrobium candidum polysaccharide extractive of embodiment 3 purifying
The Crude polysaccharides 10g that gets embodiment 1 adds 500ml water and fully dissolves, centrifugal 15 minutes of 8000r/min, the sevage reagent that supernatant liquor adds 1/5 volume extracts 3 times, 2000r/min removed precipitation in centrifugal 20 minutes, it is the ultra-filtration membrane ultrafiltration repeatedly of 10K that supernatant liquor adds after the suitable quantity of water with molecular weight, be concentrated to 200ml, concentrated solution is crossed the DEAE-52 column chromatography, last sample volume is 1/10 of a column volume, respectively with deionized water and 0.1M NaCl solution flushing twice column volume, the online detection of anthrone sulfuric acid process, first elutriant that collection obtains and second elutriant concentrated freeze-dried the two kinds of dendrobium candidum polysaccharide extractives that polysaccharide content is different of dialysing respectively, record its polysaccharide content and be respectively 46.61% (perhaps 466.1mg/g) and 32.65% (perhaps 326.5mg/g), be called dendrobium candidum polysaccharide extractive A and dendrobium candidum polysaccharide extractive B below.
Embodiment 4 contains the preparation of the granule of dendrobium candidum polysaccharide extractive
Starch 125g
Icing Sugar 60g
Sodium carboxymethylstarch 10g
Magnesium Stearate 2.5g
Talcum powder 2.5g
Ethanol 90% is an amount of
After above-mentioned auxiliary material except that 90% ethanol and Magnesium Stearate crossed 60～80 mesh sieves, mixing, mix with the prepared dendrobium candidum polysaccharide extractive of embodiment 1 again, in the gained mixture, add 95% an amount of ethanol, make softwood, pushed 20～24 mesh sieves and granulate, 40 ℃ to 65 ℃ dryings 1 hour to 4 hours, cross the whole grain of 20 mesh sieves, irritate in capsule, make 1000.
The dendrobium candidum polysaccharide extractive A 20g of embodiment 3 purifying
Sodium Benzoate 2g
Get the dendrobium candidum polysaccharide extractive A of preparation among the embodiment 3, add Sodium Benzoate, add the injection water to an amount of, heating for dissolving is put cold, add the injection water again and add to 1000ml, stir, with 0.45 μ m filtering with microporous membrane, can, seal, 100 ℃, sterilized 30 minutes, promptly.
Embodiment 6 contains the preparation of the oral liquid of dendrobium candidum polysaccharide extractive
The dendrobium candidum polysaccharide extractive B 15g of embodiment 3 purifying
Sodium Benzoate 1.5g
Get the dendrobium candidum polysaccharide extractive B of preparation among the embodiment 3, add Sodium Benzoate, add the injection water to an amount of, heating for dissolving is put cold, add the injection water again and add to 1000ml, stir, with 0.45 μ m filtering with microporous membrane, can, seal, 100 ℃, sterilized 30 minutes, promptly.
Embodiment 7 contains the preparation of the injectable lyophilised powder of dendrobium candidum polysaccharide extractive
Get the dendrobium candidum polysaccharide extractive A20g of the purifying of preparation among the embodiment 3, to wherein adding the injection water to an amount of, heating for dissolving is put cold, add the injection water again and add to 1000ml, stir, regulate pH to 6.5-7.0, with 0.22 μ m filtering with microporous membrane, aseptic subpackaged, freeze-drying, promptly.
Get the dendrobium candidum polysaccharide extractive B15g of the purifying of preparation among the embodiment 3, Xiang Qijia injection water is extremely an amount of, heating for dissolving, put cold, add the injection water again and add to 1000ml, stir, regulate pH to 6.5-7.0, with 0.22 μ m filtering with microporous membrane, can is sealed, 100 ℃, sterilized 30 minutes, promptly.
Claims (16)
1, a kind of dendrobium candidum polysaccharide extractive is characterized in that described dendrobium candidum polysaccharide extractive is that raw material extracts with the Herba Dendrobii, and its molecular weight is 1-30 ten thousand, and polysaccharide content is not less than 25% (weight).
2, dendrobium candidum polysaccharide extractive according to claim 1, the molecular weight that it is characterized in that described dendrobium candidum polysaccharide extractive is 10-30 ten thousand, polysaccharide content is 25%-50% (weight).
3, a kind of preparation method of dendrobium candidum polysaccharide extractive is characterized in that it may further comprise the steps:
(1) degreasing: after Herba Dendrobii is ground into meal,, obtain the dregs of a decoction with 10-30 times of organic solvent backflow 1-4 time;
(2) extract: the above-mentioned dregs of a decoction of boiling 1-4 time, each 1-4 hour, add water 10-30 doubly at every turn, obtain decoction liquor;
(3) concentrate: merge above-mentioned decoction liquor, be concentrated to every ml and be equivalent to contain crude drug 0.2-2g, obtain concentrated solution;
(4) alcohol precipitation: in above-mentioned concentrated solution, add an amount of ethanol and precipitate, make that containing the alcohol amount reaches 50%-80% (volume percent), with pure liquid standing over night;
(5) drying: above-mentioned pure liquid is filtered, after precipitation adds 2-3 times of organic solvent washing, drain, drying under reduced pressure obtains the dendrobium candidum polysaccharide extractive that polysaccharide content is not less than 25% (weight).
4, preparation method according to claim 3 is characterized in that the organic solvent in the described step (1) is the ethanol water mixture of sherwood oil, ethyl acetate or 80%-95%.
5, preparation method according to claim 3 is characterized in that the extraction temperature in the described step (2) is 50 ℃-100 ℃.
6, preparation method according to claim 5 is characterized in that the extraction temperature in the described step (2) is 80 ℃-100 ℃.
7, preparation method according to claim 3 is characterized in that the organic solvent in the described step (5) is sherwood oil, acetone, dehydrated alcohol, methyl alcohol, ether, chloroform or its mixture.
8, preparation method according to claim 7 is characterized in that the organic solvent in the described step (5) is a dehydrated alcohol.
9, preparation method according to claim 3 is characterized in that described preparation method also comprises following concentrated and purified step:
(1) dissolving: described dendrobium candidum polysaccharide extractive is added 20-100 times of water, and heated and stirred is fully dissolved it, and the gained liquid at high speed is centrifugal, gets supernatant liquor;
(2) deproteinated: above-mentioned supernatant liquor is diluted to 0.01-0.05g/ml, and the sevage reagent that adds 1/5 volume extracts 1-4 time, and the centrifugal precipitation of removing obtains centrifuged supernatant;
(3) ultrafiltration: with the centrifuged supernatant molecular weight that obtains is that the ultra-filtration membrane of 10K concentrates, and obtains concentrated solution;
(4) column chromatography: the concentrated solution that obtains is crossed the DEAE-52 resin column,, use the elutriant wash-out, collect the elutriant that polysaccharide content is not less than 25% (weight) with the online detection of anthrone sulfuric acid process;
(5) drying:, promptly obtain the Dendrobium officinale polysaccharide of purifying with freeze-drying behind the above-mentioned elutriant concentrating under reduced pressure;
Wherein, the elutriant in the described step (4) is deionized water and salts solution.
10, a kind of pharmaceutical composition that contains dendrobium candidum polysaccharide extractive, it is characterized in that it comprise the treatment significant quantity be the dendrobium candidum polysaccharide extractive and the pharmaceutically acceptable carrier of raw material with the Herba Dendrobii, the molecular weight of wherein said dendrobium candidum polysaccharide extractive is at 1-30 ten thousand, and polysaccharide content is not less than 25% (weight) in described Herba Dendrobii extract.
11, pharmaceutical composition according to claim 10, the formulation that it is characterized in that described pharmaceutical composition are oral solid formulation, oral semi-solid preparation, oral liquid or injection; Wherein said oral solid formulation is granule, tablet, pill, pill, hard capsule, soft capsule, solid dispersion, powder, fine granule, pellet, microcapsule, microspheres agent; Described injection is injection liquid, freeze-drying preparation for injection, sterile packaged preparation for injection, sodium chloride injection, glucose injection; Described oral liquid is an oral liquid.
12, pharmaceutical composition according to claim 11 is characterized in that described oral solid formulation is a granule.
13, pharmaceutical composition according to claim 11 is characterized in that described injection is injection liquid or injectable lyophilised powder.
14, a kind of preparation of drug combination method that contains dendrobium candidum polysaccharide extractive is characterized in that the Dendrobium officinale polysaccharide of dendrobium candidum polysaccharide extractive according to claim 1 or its purifying and pharmaceutically acceptable carrier be mixed with and forms.
15, a kind of preparation of drug combination method that contains dendrobium candidum polysaccharide extractive is characterized in that being mixed with according to the Dendrobium officinale polysaccharide of the dendrobium candidum polysaccharide extractive of each described method preparation among the claim 3-9 or its purifying and pharmaceutically acceptable carrier and forms.
16, the application of dendrobium candidum polysaccharide extractive according to claim 1 and 2 in the medicine of the disease that preparation assisting therapy hypoimmunity causes.
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