CN103300450A - Preparation method of raw dendrobium officinale pulp beverage - Google Patents
Preparation method of raw dendrobium officinale pulp beverage Download PDFInfo
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Abstract
The invention discloses a preparation method of a raw dendrobium officinale pulp beverage. The preparation method comprises the following steps of: (1), baking a fresh dendrobium officinale branch to be purple, adding water to the baked fresh dendrobium officinale branch for pulping, so as to obtain a primary serous fluid; (2), carrying out insulation treatment on the primary serous fluid, boiling and filtering the primary serous fluid, so as to obtain filter residue; (3) adding water to the filter residue for pulping, boiling and filtering the filter residue again to obtain the filter residue, repeating the sub-steps for a plurality of times, adding water to the final filter residue for the pulping, so as to obtain secondary serous fluid; (4), adding a cellulolytic enzyme into the secondary serous fluid for carrying out enzymolysis, so as to obtain an enzymolysis solution; (5) boiling and filtering the enzymolysis solution, to obtain the filter residue; (6) adding water into the filter residue for the pulping, boiling and filtering the filter residue again, to obtain the filter residue, and repeating the sub-steps for a plurality of times; (7) mixing all the filter solutions, boiling and sterilizing the mixed filter solution, so as to obtain the raw dendrobium officinale pulp beverage. The preparation method provided by the invention has the advantages that dendrobium officinale is baked, the filter residue is subjected to enzymolysis treatment, the extraction rate of functionality polyose and flavone is improved, the content of dietary fiber in the beverage is increased, and the prepared beverage color is bright and stable.
Description
Technical field
The present invention relates to the health food development technical field, be specifically related to a kind of preparation method of dendrobium candidum primary pulp drink.
Background technology
Dendrobium candidum contains dendrobium polysaccharide, flavones, protein, 17 seed amino acids and inorganic elements etc., it is sweet in flavor and cold in property, has reinforcing stomach reg fluid, nourishing Yin and clearing heat function, be described as first of " Chinese nine immortals grass ", in diseases such as treatment enterogastric diseases, cataract, arthritis, Buerger's disease and chronic pharyngitis good curative effect arranged.
The candidum tissue culturing seedling is in the high speed stage of growth with relevant health-product market, and development space is huge.According to relevant statistics, present annual dendrobium candidum market demand is more than 1,000,000 kilograms, surpasses hundred million yuan of hundreds ofs with dendrobium candidum as medicine, the health products output value of raw material.These medicines and health food seem a lot, but formulation is mostly fairly simple, and the preparation method take to the roughing of fresh goods or Tiepi Fengdou dry product as main, product is single, utilization rate of active components is lower.
Be the processing method that the Chinese patent literature of CN1307901A discloses a kind of iron-sheet dendrobe fresh product such as publication number, place Ozone Water to soak 20-40 minute fresh dendrobium candidum of gathering, then use the clear water rinsing, dry after rinsing is complete, make pulvis by the method that vacuum refrigeration is pulverized.Contain more crude fibre in the dendrobium candidum powder that described processing method obtains, edible residue is more after soaking, and mouthfeel is not good enough.
Be that the Chinese patent literature of CN1754564A discloses and a kind of the fresh dendrobium candidum young stem and leaf of gathering added honey is made honey dendrobium candidum extract processed through cleaning, fry in shallow oil juice, concentration operation method such as notification number; It is raw material that the method has adopted the dendrobium candidum young stem and leaf, but polysaccharide accumulation is not enough in the young stem and leaf, not only make in the honey dendrobium candidum extract processed of acquisition most important functional component (being polysaccharide) content less, and this valuable ingredient of traditional Chinese medicine of dendrobium candidum is caused the waste of raw material.
Publication number is the preparation method that the Chinese patent literature of CN100497396A discloses a kind of dendrobium candidum polysaccharide extractive, comprises degreasing, extraction, concentrated, alcohol precipitation, drying, and finally obtaining molecular weight is 1～300,000, and polyoses content is 25%～50%(weight).This preparation method is not used to flavones, protein, amino acid and the trace element that is rich in the dendrobium candidum, and raw material availability is also lower.
Publication number is the preparation method that the Chinese patent literature of CN102008654A discloses a kind of dendrobium officinale fresh juice stock solution, the method makes hydrolyzed pectin with large molecule hydrolyzed pectin by the separation of squeezing the juice, vacuum outgas, adding pectase and cellulase fresh dendrobium candidum washing and sterilizing, to reduce viscosity.But pectase is processed and also can with polysaccharide hydrolysis, be made the dendrobium officinale fresh juice stock solution of acquisition lose due effect.
Summary of the invention
The invention provides a kind of preparation method of dendrobium candidum primary pulp drink, be rich in the functional components such as polysaccharide, flavones in the dendrobium candidum primary pulp drink that utilizes this preparation method to obtain, and bright-colored stable, and the utilization rate of dendrobium candidum is up to more than 70%.
A kind of preparation method of dendrobium candidum primary pulp drink comprises:
(1) gets the bright branch of dendrobium candidum, be baked to and be purple, add the water making beating, make just slurries;
(2) with boiling after the first slurries insulation processing, filter, get filter residue;
(3) filter residue is added again the water making beating, boil, filter, get filter residue, after the repeated several times, filter residue adds the water making beating and makes the secondary slurries;
(4) add cellulase in the secondary slurries and carry out enzymolysis, obtain enzymolysis liquid;
(5) enzymolysis liquid is boiled, filters, get filter residue,
(6) filter residue is added again the water making beating, boil, filter, get filter residue, repeated several times;
(7) merge all filtrates, boil sterilization, make the dendrobium candidum primary pulp drink.
In the step (1), add water making beating before, the bright branch of the dendrobium candidum after the baking is cut into chunks length 0.1～1.0cm; 0.2cm more preferably.Shorter dendrobium candidum stem section more is conducive to the stripping of functional polysaccharide and flavones.
As preferably, baking temperature is 85～95 ℃, and stoving time is 10～30min.As further preferred, baking temperature is 95 ℃, and stoving time is 15min.High-temperature baking can not only be removed herbaceous taste, plays the effect that completes; And in the bake process, the anthocyanin in the dendrobium candidum and functional polysaccharide, flavones reaction make the color of primary pulp drink more bright-coloured and stable.
After baking is finished, add clean clear water making beating, make functional polysaccharide, flavones and anthocyanin stripping in the dendrobium candidum.When adding the water making beating, material-water ratio is preferably 1:5～7.
For making more anthocyanin stripping, in the step (2), the first slurries that obtain are incubated a period of time at a certain temperature, heat-retaining condition is preferably: 50～55 ℃ of temperature, time 30～120min; More preferably: temperature 50 C, time 30min.Under this heat-retaining condition, substantially stripping of anthocyanin.
In the step (3), the water that adds 8～10 times of amounts of dendrobium candidum raw material in the first slurries after insulation, filter after boiling 3～8min, get the filter residue after the filtration, the water making beating that adds 3～5 times of amounts of filter residue, obtain the secondary slurries, as without special appointment, dendrobium candidum raw material of the present invention refers to bright of dendrobium candidum.
Slurries are boiled not only play the effect of sterilization but also greatly improved functional polysaccharide, flavones extraction efficiency.As preferably, in the step (3), the number of times of described repetition is 3 times at least, repeatedly adds the water making beating and boils the recovery rate that can further improve functional polysaccharide, flavones.
Through after repeatedly adding water making beating and boiling, most of free functional polysaccharide, substantially stripping of flavones, recovery rate can't improve again.But because the main component of filter residue is cellulose, also fetter some functional polysaccharide, flavones between the cellulosic molecule, and cellulose is water insoluble and common organic solvents, so that this part bound functional polysaccharide, flavones also can't stripping.
Therefore in the step of the present invention (4), in the secondary slurries, add cellulase, utilize cellulase that cellulose is carried out enzymolysis.As preferably, the addition of described cellulase is 5-15U/g dendrobium candidum raw material, more preferably 10U/g dendrobium candidum raw material.1U refers at 50 ℃, pH5.0, and under the condition of reaction 30min, per minute degraded CMC(carboxymethyl cellulose) the required enzyme amount of generation 1 μ g reduced sugar (with glucose meter).
As preferably, enzymolysis time is 10～40min, and enzymolysis pH is 4.8～5.2, and hydrolysis temperature is 45～55 ℃.As further preferred, enzymolysis time is 30min, and enzymolysis pH is 5.0, and hydrolysis temperature is 50 ℃.Be equipped with stirring during enzymolysis, to increase the contact area of filter residue and cellulase, improve enzymolysis efficiency.Behind the enzymolysis, the functional polysaccharide, the flavones that are not only fettered by cellulose are released, and have also obtained many cellulosic water-soluble enzymolysis things.
After the stirring enzymolysis is finished enzymolysis liquid being boiled 3～8min also filters.Get the filter residue after the filtration, add the water making beating of 3～5 times of amounts of filter residue.As preferably, in the step (6), the number of times of described repetition is 3 times at least, repeatedly adds the water making beating and boils the recovery rate that can further improve functional polysaccharide, flavones.
As preferably, in the boiling part of step (3) and step (6), in slurries, add an amount of dried peppermint leaf.The dendrobium candidum primary pulp drink that so obtains not only has the fragrance of dendrobium candidum, also has the refrigerant mouthfeel of peppermint, and more being of value to promotes the production of body fluid quenches one's thirst, the moistening lung health.
The present invention also provides the dendrobium candidum that is made by described preparation method primary pulp drink.In this dendrobium candidum primary pulp drink, anthocyanin content＞0.3 μ g/mL, flavones content＞0.4 μ g/mL, functional polysaccharide content＞1.5mg/mL.
Compared with prior art, beneficial effect of the present invention is:
(1) carries out again the extraction of functional component after the present invention toasts dendrobium candidum, be conducive to functional polysaccharide, flavones stripping, and make the dendrobium candidum primary pulp drink that makes bright-colored, stable;
(2) the present invention utilizes cellulase that cellulose is carried out enzymolysis, increased on the one hand the recovery rate to compositions such as functional polysaccharides in the dendrobium candidum, make on the other hand the cellulose Partial digestion, be dissolved in the soluble dietary fiber that becomes body health benefits in the beverage, also can too far consequently do not cause to the functional polysaccharide degraded simultaneously the reduction of health-care efficacy;
(3) preparation method of the present invention is simple to operate, and energy-conserving and environment-protective, cost are low; Functional component rich content in the dendrobium candidum primary pulp drink that makes has good health-care efficacy.
Description of drawings
Fig. 1 is the preparation method's of dendrobium candidum primary pulp drink of the present invention flow chart.
The specific embodiment
The preparation flow of dendrobium candidum primary pulp drink of the present invention is described in further detail preparation method of the present invention below in conjunction with the drawings and specific embodiments as shown in Figure 1.
Embodiment 1
(1) with fresh dendrobium candidum defoliation, clean, dry, behind 85 ℃ of lower baking 40min, be cut into the stem section that length is 0.1cm; Getting material-water ratio is dendrobium candidum stem section and the clean clear water of 1:6, and making beating obtains just slurries;
(2) with first slurries insulation 60min under 55 ℃; The water that adds 8 times of amounts of dendrobium candidum raw material after the insulation filters after boiling 3min, and filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 4 times of amounts, making beating obtains the secondary slurries;
(3) add cellulase in the secondary slurries, addition is 5U/g dendrobium candidum raw material; At about 5.0, the 48 ℃ of lower enzymolysis 20min of pH, obtain enzymolysis liquid;
(4) filter after enzymolysis liquid is boiled 3min, filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 3 times of amounts, pull an oar, filter, get filtrate;
(5) merge all filtrate, boil, make the dendrobium candidum primary pulp drink; And the bright branch utilization rate of calculating dendrobium candidum; Computing formula is:
In the formula, M is the bright branch of dendrobium candidum moisture for the bright branch weight of the front dendrobium candidum of baking, x, m
1Be final filter residue dry weight.
The bright branch utilization rate of dendrobium candidum is 77% in the present embodiment.
Embodiment 2
(1) with fresh dendrobium candidum defoliation, clean, dry, behind 95 ℃ of lower baking 15min, be cut into the stem section that length is 0.2cm; Getting material-water ratio is dendrobium candidum stem section and the clean clear water of 1:5, and making beating obtains just slurries;
(2) with first slurries insulation 30min under 50 ℃; The water that adds 10 times of amounts of dendrobium candidum raw material after the insulation filters after boiling 5min, and filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 3 times of amounts, making beating obtains the secondary slurries;
(3) add cellulase in the secondary slurries, addition is 10U/g dendrobium candidum raw material; At about 5.0, the 50 ℃ of lower enzymolysis 30min of pH, obtain enzymolysis liquid;
(4) filter after enzymolysis liquid is boiled 5min, filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 4 times of amounts, pull an oar, filter, get filtrate;
(5) merge all filtrate, sterilization makes the dendrobium candidum primary pulp drink; And utilize the method for embodiment 1 that the bright branch utilization rate of the present embodiment is calculated, be 77%.
Embodiment 3
(1) with fresh dendrobium candidum defoliation, clean, dry, behind 90 ℃ of lower baking 30min, be cut into the stem section that length is 0.8cm; Getting material-water ratio is dendrobium candidum stem section and the clean clear water of 1:7, and making beating obtains slurries;
(2) with first slurries insulation 120min under 50 ℃; The water that adds 9 times of amounts of dendrobium candidum raw material after the insulation filters after boiling 5min, and filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 5 times of amounts, making beating obtains the secondary slurries;
(3) add cellulase in the secondary slurries, addition is 15U/g dendrobium candidum raw material; Be about 5.0,52 ℃ of lower enzymolysis 10min at pH, obtain enzymolysis liquid;
(4) filter after enzymolysis liquid is boiled 5min, filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 5 times of amounts, pull an oar, filter, get filtrate;
(5) merge all filtrate, sterilization makes the dendrobium candidum primary pulp drink; And utilize the method for embodiment 1 that the bright branch utilization rate of the present embodiment is calculated, be 72%.
Embodiment 4
(1) with fresh dendrobium candidum defoliation, clean, dry, behind 85 ℃ of lower baking 40min, be cut into the stem section that length is 1cm; Getting material-water ratio is dendrobium candidum stem section and the clean clear water of 1:7, and making beating obtains slurries;
(2) with first slurries insulation 100min under 55 ℃; The water that adds 10 times of amounts of dendrobium candidum raw material after the insulation filters after boiling 8min, and filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 5 times of amounts, making beating obtains the secondary slurries;
(3) add cellulase in the secondary slurries, addition is 15U/g dendrobium candidum raw material; Be about 5.0,50 ℃ of lower enzymolysis 40min at pH, obtain enzymolysis liquid;
(4) filter after enzymolysis liquid is boiled 8min, filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 5 times of amounts, pull an oar, filter, get filtrate;
(5) merge all filtrate, sterilization makes the dendrobium candidum primary pulp drink; And utilize the method for embodiment 1 that the bright branch utilization rate of the present embodiment is calculated, be 71%.
Embodiment 5
(1) with fresh dendrobium candidum defoliation, clean, dry, behind 95 ℃ of lower baking 20min, be cut into the stem section that length is 0.2cm; Getting material-water ratio is dendrobium candidum stem section and the clean clear water of 1:6, and making beating obtains just slurries;
(2) with first slurries insulation 120min under 55 ℃; The water that adds 8 times of amounts of dendrobium candidum raw material after the insulation filters after boiling 3min, and filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 4 times of amounts, making beating obtains the secondary slurries;
(3) add cellulase in the secondary slurries, addition is 10U/g dendrobium candidum raw material; Be about 5.0,48 ℃ of lower enzymolysis 10min at pH, obtain enzymolysis liquid;
(4) filter after enzymolysis liquid is boiled 3min, filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 3 times of amounts, pull an oar, filter, get filtrate;
(5) merge all filtrate, sterilization makes the dendrobium candidum primary pulp drink; And utilize the method for embodiment 1 that the bright branch utilization rate of the present embodiment is calculated, be 75%.
Embodiment 6
(1) with fresh dendrobium candidum defoliation, clean, dry, behind 90 ℃ of lower baking 15min, be cut into the stem section that length is 0.1cm; Getting material-water ratio is dendrobium candidum stem section and the clean clear water of 1:5, and making beating obtains just slurries;
(2) with first slurries insulation 120min under 55 ℃; The water that adds 8 times of amounts of dendrobium candidum raw material after the insulation filters after boiling 3min, and filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 4 times of amounts, making beating obtains the secondary slurries;
(3) add cellulase in the secondary slurries, addition is 15U/g dendrobium candidum raw material; Be about 5.0,52 ℃ of lower enzymolysis 10min at pH, obtain enzymolysis liquid;
(4) filter after enzymolysis liquid is boiled 3min, filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 3 times of amounts, pull an oar, filter, get filtrate;
(5) merge all filtrate, sterilization makes the dendrobium candidum primary pulp drink; And utilize the method for embodiment 1 that the bright branch utilization rate of the present embodiment is calculated, be 78%.
Embodiment 7
(1) with fresh dendrobium candidum defoliation, clean, dry, behind 85 ℃ of lower baking 30min, be cut into the stem section that length is 0.5cm; Getting material-water ratio is dendrobium candidum stem section and the clean clear water of 1:7, and making beating obtains just slurries;
(2) with first slurries insulation 30min under 52 ℃; The water that adds 9 times of amounts of dendrobium candidum raw material after the insulation filters after boiling 3min, and filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 5 times of amounts, making beating obtains the secondary slurries;
(3) add cellulase in the secondary slurries, addition is 5U/g dendrobium candidum raw material; Be about 5.0,50 ℃ of lower enzymolysis 20min at pH, obtain enzymolysis liquid;
(4) filter after enzymolysis liquid is boiled 3min, filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 3 times of amounts, pull an oar, filter, get filtrate;
(5) merge all filtrate, sterilization makes the dendrobium candidum primary pulp drink; And utilize the method for embodiment 1 that the bright branch utilization rate of the present embodiment is calculated, be 72%.
Embodiment 8
(1) with fresh dendrobium candidum defoliation, clean, dry, behind 85 ℃ of lower baking 15min, be cut into the stem section that length is 0.3cm; Getting material-water ratio is dendrobium candidum stem section and the clean clear water of 1:6, and making beating obtains just slurries;
(2) with first slurries insulation 30min under 50 ℃; The water that adds 9 times of amounts of dendrobium candidum raw material after the insulation filters after boiling 5min, and filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 4 times of amounts, making beating obtains the secondary slurries;
(3) add cellulase in the secondary slurries, addition is 10U/g dendrobium candidum raw material; Be about 5.0,50 ℃ of lower enzymolysis 30min at pH, obtain enzymolysis liquid;
(4) filter after enzymolysis liquid is boiled 5min, filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 5 times of amounts, pull an oar, filter, get filtrate;
(5) merge all filtrate, sterilization makes the dendrobium candidum primary pulp drink; And utilize the method for embodiment 1 that the bright branch utilization rate of the present embodiment is calculated, be 75%.
Embodiment 9
(1) with fresh dendrobium candidum defoliation, clean, dry, behind 95 ℃ of lower baking 20min, be cut into the stem section that length is 0.2cm; Getting material-water ratio is dendrobium candidum stem section and the clean clear water of 1:7, and making beating obtains just slurries;
(2) with first slurries insulation 60min under 50 ℃; The water that adds 10 times of amounts of dendrobium candidum raw material after the insulation filters after boiling 8min, and filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 5 times of amounts, making beating obtains the secondary slurries;
(3) add cellulase in the secondary slurries, addition is 10U/g dendrobium candidum raw material; Be about 5.0,50 ℃ of lower enzymolysis 30min at pH, obtain enzymolysis liquid;
(4) filter after enzymolysis liquid is boiled 8min, filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 5 times of amounts, pull an oar, filter, get filtrate;
(5) merge all filtrate, sterilization makes the dendrobium candidum primary pulp drink; And utilize the method for embodiment 1 that the bright branch utilization rate of the present embodiment is calculated, be 76%.
Embodiment 10
(1) with fresh dendrobium candidum defoliation, clean, dry, behind 95 ℃ of lower baking 30min, be cut into the stem section that length is 1cm; Getting material-water ratio is dendrobium candidum stem section and the clean clear water of 1:7, and making beating obtains just slurries;
(2) with first slurries insulation 60min under 50 ℃; The water that adds 9 times of amounts of dendrobium candidum raw material after the insulation filters after boiling 5min, and filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 3 times of amounts, making beating obtains the secondary slurries;
(3) add cellulase in the secondary slurries, addition is 5U/g dendrobium candidum raw material; Be about 5.0,55 ℃ of lower enzymolysis 40min at pH, obtain enzymolysis liquid;
(4) filter after enzymolysis liquid is boiled 5min, filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 4 times of amounts, pull an oar, filter, get filtrate;
(5) merge all filtrate, sterilization makes the dendrobium candidum primary pulp drink; And utilize the method for embodiment 1 that the bright branch utilization rate of the present embodiment is calculated, be 70%.
Embodiment 11
(1) with fresh dendrobium candidum defoliation, clean, dry, behind 85 ℃ of lower baking 15min, be cut into the stem section that length is 0.8cm; Getting material-water ratio is dendrobium candidum stem section and the clean clear water of 1:6, and making beating obtains just slurries;
(2) with first slurries insulation 120min under 50 ℃; The water that adds 10 times of amounts of dendrobium candidum raw material after the insulation filters after boiling 8min, and filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 5 times of amounts, making beating obtains the secondary slurries;
(3) add cellulase in the secondary slurries, addition is 10U/g dendrobium candidum raw material; Be about 5.0,45 ℃ of lower enzymolysis 40min at pH, obtain enzymolysis liquid;
(4) filter after enzymolysis liquid is boiled 8min, filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 5 times of amounts, pull an oar, filter, get filtrate;
(5) merge all filtrate, sterilization makes the dendrobium candidum primary pulp drink; And utilize the method for embodiment 1 that the bright branch utilization rate of the present embodiment is calculated, be 74%.
Embodiment 12
(1) with fresh dendrobium candidum defoliation, clean, dry, behind 85 ℃ of lower baking 20min, be cut into the stem section that length is 0.2cm; Getting material-water ratio is dendrobium candidum stem section and the clean clear water of 1:7, and making beating obtains just slurries;
(2) with first slurries insulation 30min under 55 ℃; The water that adds 8 times of amounts of dendrobium candidum raw material after the insulation filters after boiling 3min, and filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 3 times of amounts, making beating obtains the secondary slurries;
(3) add cellulase in the secondary slurries, addition is 15U/g dendrobium candidum raw material; Be about 5.0,50 ℃ of lower enzymolysis 20min at pH, obtain enzymolysis liquid;
(4) filter after enzymolysis liquid is boiled 5min, filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 4 times of amounts, pull an oar, filter, get filtrate;
(5) merge all filtrate, sterilization makes the dendrobium candidum primary pulp drink; And utilize the method for embodiment 1 that the bright branch utilization rate of the present embodiment is calculated, be 75%.
Embodiment 13
(1) with fresh dendrobium candidum defoliation, clean, dry, behind 95 ℃ of lower baking 15min, be cut into the stem section that length is 1cm; Getting material-water ratio is dendrobium candidum stem section and the clean clear water of 1:6, and making beating obtains just slurries;
(2) with first slurries insulation 60min under 50 ℃; The water that adds 10 times of amounts of dendrobium candidum raw material after the insulation filters after boiling 8min, and filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 5 times of amounts, making beating obtains the secondary slurries;
(3) add cellulase in the secondary slurries, addition is 10U/g dendrobium candidum raw material; Be about 5.0,55 ℃ of lower enzymolysis 30min at pH, obtain enzymolysis liquid;
(4) filter after enzymolysis liquid is boiled 5min, filtrate is preserved; To filter residue repetition " adding water to pull an oar-boil-filter " three times, get the water that filter residue adds 4 times of amounts, pull an oar, filter, get filtrate;
(5) merge all filtrate, sterilization makes the dendrobium candidum primary pulp drink; And utilize the method for embodiment 1 that the bright branch utilization rate of the present embodiment is calculated, be 71%.
Content to functional polysaccharide and flavones in the dendrobium candidum primary pulp drink of embodiment 1～13 acquisition is measured, and measurement result is as shown in table 1.
The content of polysaccharide and flavones in each embodiment dendrobium candidum primary pulp drink of table 1
The dendrobium candidum primary pulp drink color that above embodiment makes is all bright-coloured stable, the content of comprehensive function polysaccharide and flavones, and the dendrobium candidum primary pulp drink that embodiment 2 obtains is best.
Comparative Examples 1
Utilize respectively the method identical with embodiment 1～13 to prepare the dendrobium candidum primary pulp drink, but fresh dendrobium candidum directly add water without baking and pull an oar in the step (1).Content to functional polysaccharide, flavones in the dendrobium candidum primary pulp drink that obtains detects, and testing result is as shown in table 2.
Table 2 baking dendrobium candidum stem section is on the impact of extraction effect
Claims (10)
1. the preparation method of a dendrobium candidum primary pulp drink comprises:
(1) gets the bright branch of dendrobium candidum, be baked to and be purple, add the water making beating, make just slurries;
(2) with boiling after the first slurries insulation processing, filter, get filter residue;
(3) filter residue is added again the water making beating, boil, filter, get filter residue, after the repeated several times, filter residue adds the water making beating and makes the secondary slurries;
(4) add cellulase in the secondary slurries and carry out enzymolysis, obtain enzymolysis liquid;
(5) enzymolysis liquid is boiled, filters, get filter residue,
(6) filter residue is added again the water making beating, boil, filter, get filter residue, repeated several times;
(7) merge all filtrates, boil sterilization, make the dendrobium candidum primary pulp drink.
2. preparation method as claimed in claim 1 is characterized in that, in the step (1), add water making beating before, the bright branch of the dendrobium candidum after the baking is cut into chunks length 0.1～1.0cm.
3. preparation method as claimed in claim 1 is characterized in that, in the step (1), baking temperature is 85～95 ℃, and stoving time is 10～30min.
4. preparation method as claimed in claim 1 is characterized in that, in the step (2), the temperature that insulation is processed is 50～55 ℃, time 30～120min.
5. preparation method as claimed in claim 1 is characterized in that, in the step (4), the addition of cellulase is 5-15U/g dendrobium candidum raw material.
6. preparation method as claimed in claim 1 is characterized in that, in the step (4), enzymolysis time is 10～40min, and enzymatic hydrolysis system pH is 4.8～5.2, and hydrolysis temperature is 45～55 ℃.
7. preparation method as claimed in claim 1 is characterized in that, in the step (3), the number of times of described repetition is 3 times at least.
8. preparation method as claimed in claim 1 is characterized in that, in the step (6), the number of times of described repetition is 3 times at least.
9. the dendrobium candidum primary pulp drink that makes such as the arbitrary described preparation method of claim 1～8.
10. dendrobium candidum primary pulp drink as claimed in claim 9 is characterized in that, anthocyanin content＞0.3 μ g/mL, flavones content＞0.4 μ g/mL, functional polysaccharide content＞1.5mg/mL.
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