CN107496885A - A kind of preparation method for the dendrobium candidum Chinese medicine preparation for treating osteoporosis - Google Patents
A kind of preparation method for the dendrobium candidum Chinese medicine preparation for treating osteoporosis Download PDFInfo
- Publication number
- CN107496885A CN107496885A CN201710909895.4A CN201710909895A CN107496885A CN 107496885 A CN107496885 A CN 107496885A CN 201710909895 A CN201710909895 A CN 201710909895A CN 107496885 A CN107496885 A CN 107496885A
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- China
- Prior art keywords
- parts
- dendrobium candidum
- extract
- chinese medicine
- root
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Abstract
A kind of preparation method for the dendrobium candidum Chinese medicine preparation for treating osteoporosis, including dendrobium candidum is squeezed the juice to obtain squeezeding juice, is heated to reflux after being diluted with water, then concentrated dendrobium candidum extract solution；Tortoiseshell, small lycopod, sheep hinge and the root of herbaceous peony are taken, adds ethanol, refluxing extraction, then be condensed into medicinal extract；Ginger and radix glycyrrhizae are taken, steam distillation obtains the dregs of a decoction, volatile oil and the aqueous solution；The dregs of a decoction and root of bidentate achyranthes etc. are mixed, and added water to cook, filtrate is obtained after filtering；The aqueous solution is merged with filtrate, is condensed into clear cream；Dendrobium candidum extract solution, medicinal extract are merged with clear cream, dry extract is obtained after vacuum drying；By magnesium stearate, talcum powder, drug extract powder, volatile oil merges with beta cyclodextrin, mixes, pelletizing, be whole grain, tabletted, then producing Chinese medicine preparation after carrying out film or sweet tablet.The dendrobium candidum Chinese medicine preparation for the treatment of osteoporosis produced by the present invention effectively limit bone can convert, and maintain the balance of bone information and bon e formation, improve the function of osteocyte, increase bone amount.
Description
Technical field
The present invention relates to a kind of Chinese medicine preparation, more particularly to a kind of system for the dendrobium candidum Chinese medicine preparation for treating osteoporosis
Preparation Method, belong to medicinal chemistry art.
Background technology
In theory of traditional Chinese medical science, viscera material function reflects the biological phenomena of people, the wherein turbid resistance of hemostasis phlegm of kidney deficiency folder
Network is basic pathology in aging.The performance of aging includes the phenomenon of form aging, internal organ aging and function aging；Aging
Mechanism is said including congenital heredity, the day after tomorrow takes good care of oneself and main void says three ends, wherein thinking that kidney deficiency is the master of aging in terms of main empty theory
Cause.Kidney qi prosperity and decline plays a crucial role in human body prosperity and decline, and the main Physiological Function of kidney is store essential substances, and main bone gives birth to marrow.
The kidney generating marrow and dominating bone refers to that kidney essense has and promotes bone growth and development, grows the effect of marrow, spinal cord and brains.Kidney is hidden
Essence, essence can give birth to marrow, and marrow is occupied in bone, and bone relies marrow to supplement nutrition.Kidney essense is sufficient, then marrow biochemistry is active, and marrow fills, and bone obtains marrow taste
Support and develop normally, it is strong.Marrow, spinal cord and brains have essence in kidney institute metaplasia, pass through brain on spinal cord, brain is marrow
Sea, formed by marrow is poly-.So existence and the correlation function of bone, marrow, brain, all inseparable with kidney essense.
Strong muscle zhuanggu is one of content of daily health caring, and the elderly's relatively concern.With social population
Aging, osteoporosis become increasingly common, are classified as one of three big diseases of old people by WHO, osteoporosis should be adhered to
Put prevention first, prevent and treat the principle combined.《Ancient Chinese medical classics essential ideas》Described in " kidney storing essence, the raw marrow of essence, marrow life bone, essence foot then marrow foot, marrow foot
Then bone is strong ".If kidney-jing deficiency is few, medullization source deficiency, sclerotin will occurs and lose foster, initiation osteoporosis.Osteoporosis is a kind of
Multi-pathogenesis disease, its basic pathology mechanism are that the coupling of bone information and bon e formation during Bone m etabolism defect occurs.At present
Built again through knowing in body circulation in a variety of hormones, Bone m etabolism that some local modulation factors etc. may all influence Bone m etabolism and bone
Journey.
Osteoporosis shows as solid content reduction, bone micro-structure changes so that fracture risk increase, is old in threatening
A kind of common metabolic osteopathy of year people health, and a kind of metabolic disease characterized by bone tissue fibre structure is degenerated,
It is the state that bone unit volume bone amount reduces.Though " osteoporosis " is not referred in Basic Theories of Chinese Medicine clearly, in the successive dynasties
Cure document on the description of the related disease feature such as " bone withers ", " bone is withered ", " heumatism " such as aching pain in waist and back, height shorten, it is bow-backed and
The change of fracture, it is very much like with the clinical symptoms and sign of modern medicine osteoporosis, wherein qualitative, fixed with " bone withers "
Position is more accurate.Theory of traditional Chinese medical science thinks that the main pathogenesis that osteoporosis occurs is deficiency of the kidney, insufficiency of the spleen and blood stasis.Kidney for it is congenital it
This, main store essential substances, main bone gives birth to marrow, kidney deficiency marrow sky then dysequilibrium, therefore, the growing of bone, it is powerful it is weak with kidney essense prosperity and decline
There is close relationship.Then marrow biochemistry is active for kidney essense abundance, and bone obtains nourishing strong, and otherwise deficiency of kidney-essence then give birth to by marrow
Change weary source, bone, which loses, supports, and bone mineral content declines, and bone density reduces, and osteoporosis occurs.
The method of preventing and treating senile osteoporosis is more but ineffective at present.If controversies in hormone replacement in the elderly is exhausted in women
Effective use of osteoporosis after, make the HRT attention of Male Osteoporosis, mainly by ovary point
The estrogen secreted can be changed by the ERs of Gegenbaur's cell and osteoclast come limit bone, to maintain bone information and bone shape
Into balance, and directly act on the acceptor of Gegenbaur's cell, improve the function of Gegenbaur's cell, increase bone amount.But due to hormone
Some side effects can be derived so unsuitable long-term use of.
The content of the invention
It is an object of the invention to provide a kind of preparation method for the dendrobium candidum Chinese medicine preparation for treating osteoporosis.
In order to achieve the above objects and other related objects, technical scheme provided by the invention is：One kind treats osteoporosis
Dendrobium candidum Chinese medicine preparation preparation method, the composition of raw materials of dendrobium candidum Chinese medicine preparation of the treatment osteoporosis includes
The component of following parts by weight：
Preparation method comprises the following steps：
The first step：Squeezed the juice after fresh dendrobium candidum is mixed with water, then with 200 mesh filtered through gauze, obtained filtrate is micro-
Boiling preserves under the conditions of being placed in 4 DEG C after 3~5 minutes, obtains squeezeding juice；
Second step：It is 50~200mg/mL that the squeezeding juice is diluted with water into solid content, then heating and refluxing extraction 2~
5 times, in heating and refluxing extraction, each micro-boiling 0.5~1.5 hour, merge extract solution, next concentrate institute with Rotary Evaporators
State extract solution and obtain concentrate, the water of 1.5~3 times of weight is added in the most backward concentrate, is stood overnight, after next day filtering
Obtained filtrate is dendrobium candidum extract solution；
3rd step：Tortoiseshell, small lycopod, sheep hinge and the root of herbaceous peony are taken, the volume fraction for adding 3~15 times of volumes is 50~95%
Ethanol, refluxing extraction 1~6 time, 0.5~3 hour every time, merge ethanol extract, the filtrate obtained after filtering is condensed into phase again
To the medicinal extract that density is 1.25~1.35, relative density measures when 60 DEG C；
4th step：Ginger and radix glycyrrhizae are taken, adds the water of 5~20 times of volumes to soak 0.5~24 hour, steam distillation 0.5~
12 hours, condensate liquid and the dregs of a decoction are obtained, the condensate liquid obtains volatile oil and the aqueous solution after water-oil separating；
5th step：The dregs of a decoction obtained by 4th step are mixed with the root of bidentate achyranthes, subprostrate sophora, the fruit of medicinal cornel, barrenwort, shrubby woodfordia root and flower, and to its
The middle decocting for adding 5~20 times of volumes is boiled 1~6 time, and 1~3 hour every time, filtrate is obtained after collecting decoction filtering；
6th step：The aqueous solution collected by 4th step is merged with the filtrate obtained by the 5th step, being condensed into relative density is
1.25~1.35 clear cream, relative density measure when 60 DEG C；
7th step：Dendrobium candidum extract solution, medicinal extract are merged with clear cream, dry extract is obtained after vacuum drying, by the dry leaching
Cream crushes, and it is drug extract powder to cross after 60~100 mesh sieves obtained screenings；By magnesium stearate, talcum powder, the drug extract
The volatile oil that powder, the 4th step obtain merges with beta-schardinger dextrin, mixed, pelletizing, whole grain, tabletted, then carries out film or sugar bag
The dendrobium candidum Chinese medicine preparation for the treatment of osteoporosis is produced after clothing.
Preferably technical scheme is：The composition of raw materials of the dendrobium candidum Chinese medicine preparation of the treatment osteoporosis includes following
The component of parts by weight：
Preferably technical scheme is：Preparation method comprises the following steps：
The first step：Squeezed the juice after fresh dendrobium candidum is mixed with water, then with 200 mesh filtered through gauze, obtained filtrate is micro-
Boiling preserves under the conditions of being placed in 4 DEG C after 4 minutes, obtains squeezeding juice；
Second step：It is 160mg/mL that the squeezeding juice is diluted with water into solid content, then heating and refluxing extraction 3 times,
During heating and refluxing extraction, each micro-boiling 1.1 hours, merge extract solution, next concentrating the extract solution with Rotary Evaporators obtains
To concentrate, the water of 2.6 times of weight is most added in the concentrate backward, is stood overnight, the filtrate obtained after next day filtering is
Dendrobium candidum extract solution；
3rd step：Tortoiseshell, small lycopod, sheep hinge and the root of herbaceous peony are taken, the ethanol that the volume fraction for adding 12 times of volumes is 75%, is returned
Stream extraction 4 times, 2.2 hours every time, merge ethanol extract, it is 1.32 that the filtrate obtained after filtering is condensed into relative density again
Medicinal extract, relative density measure when 60 DEG C；
4th step：Ginger and radix glycyrrhizae are taken, adds the water of 16 times of volumes to soak 13 hours, steam distillation 8 hours, is condensed
Liquid and the dregs of a decoction, the condensate liquid obtain volatile oil and the aqueous solution after water-oil separating；
5th step：The dregs of a decoction obtained by 4th step are mixed with the root of bidentate achyranthes, subprostrate sophora, the fruit of medicinal cornel, barrenwort, shrubby woodfordia root and flower, and to its
The middle decocting for adding 16 times of volumes is boiled 5 times, and 2 hours every time, filtrate is obtained after collecting decoction filtering；
6th step：The aqueous solution collected by 4th step is merged with the filtrate obtained by the 5th step, being condensed into relative density is
1.3 clear cream, relative density measure when 60 DEG C；
7th step：Dendrobium candidum extract solution, medicinal extract are merged with clear cream, dry extract is obtained after vacuum drying, by the dry leaching
Cream crushes, and it is drug extract powder to cross after 80 mesh sieves obtained screenings；By magnesium stearate, talcum powder, the drug extract powder,
The volatile oil that four steps obtain merges with beta-schardinger dextrin, mixed, pelletizing, whole grain, tabletted, then carries out after film or sweet tablet i.e.
The dendrobium candidum Chinese medicine preparation of osteoporosis must be treated.
Because above-mentioned technical proposal is used, the present invention this have the advantage that compared with prior art：
(1) the dendrobium candidum Chinese medicine preparation for the treatment of osteoporosis produced by the present invention effectively limit bone can convert, and maintain
The balance of bone information and bon e formation, the function of osteocyte is improved, increase bone amount.
(2) Chinese medicine preparation of the invention can be long-term use of.
(3) method of the invention can fully extract the active polysaccharide in dendrobium candidum, anti-using it to greatest extent
The activity of oxide group.
Embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this implementation
Content disclosed by example understands other advantages and effect of the present invention easily.
Embodiment 1：A kind of preparation method for the dendrobium candidum Chinese medicine preparation for treating osteoporosis
A kind of dendrobium candidum Chinese medicine preparation for treating osteoporosis, by weight, 26 parts of dendrobium candidum extract solution, tortoiseshell
12 parts, 12 parts of small lycopod, 9 parts of sheep hinge, 8 parts of the root of herbaceous peony, 7 parts of the root of bidentate achyranthes, 5 parts of subprostrate sophora, 12 parts of the fruit of medicinal cornel, 13 parts of barrenwort, shrimp-roe
Spend 10 parts, 7 parts of ginger, 12 parts of radix glycyrrhizae.
The formulation of the dendrobium candidum Chinese medicine preparation of the treatment osteoporosis is tablet.
Preparation method comprises the following steps：
(1) fresh dendrobium candidum is weighed, cleans, cut section, into juice extractor, by several times plus appropriate amount of water is distilled, squeezes the juice, with 200
Mesh filter-cloth filtering, after filtrate is heated to boiling, 4 DEG C of preservations are placed in after being maintained at slight boiling condition lower 3 minutes, obtain squeezeding juice.
(2) squeezeding juice for taking step (1) to obtain, it is 50mg/mL to be diluted with water to solid content, heating and refluxing extraction 2 times,
During heating and refluxing extraction, each micro-boiling 0.5 hour (i.e. squeezeding juice carries out refluxing extraction under slight boiling condition), merge extract solution,
Concentrate is obtained with Rotary Evaporators concentrated extracting solution, the water of 1.5 times of weight is added in the most backward concentrate, was placed
At night, the filtrate obtained after next day filtering is dendrobium candidum extract solution.
(3) tortoiseshell, small lycopod, sheep hinge and the root of herbaceous peony are taken, the ethanol that the volume fraction for adding 3 times of volumes is 50%, backflow carries
Taking 1 time, 0.5 hour every time, merge ethanol extract, the filtrate being filtrated to get is condensed into the medicinal extract that relative density is 1.25 again,
Relative density is measured when 60 DEG C, and ethanol is reclaimed in concentration process.
(4) ginger and radix glycyrrhizae are taken, adds the water of 5 times of volumes to soak 0.5 hour, steam distillation 0.5 hour, obtains condensate liquid
And the dregs of a decoction, the condensate liquid obtain volatile oil and the aqueous solution after water-oil separating.
(5) dregs of a decoction obtained by step (4) are mixed with the root of bidentate achyranthes, subprostrate sophora, the fruit of medicinal cornel, barrenwort and shrubby woodfordia root and flower, and thereto
The decocting for adding 5 times of volumes is boiled 1 time, 1 hour every time, collecting decoction, filtrate is obtained after filtering.
(6) aqueous solution that step (4) is collected is merged with the filtrate obtained by step (5), is condensed into relative density as 1.25
Clear cream, what relative density was surveyed when 60 DEG C.
(7) medicinal extract that clear cream, the step (3) obtained dendrobium candidum extract solution, step (6) obtains merges, vacuum drying
Dry extract is obtained afterwards, the dry extract is crushed, and it is drug extract powder to cross after 60 mesh sieves obtained screenings；Take magnesium stearate, cunning
Volatile oil, the beta-schardinger dextrin that stone flour and said medicine extract powder, step (4) obtain merge, mixed, pelletizing, whole grain, are pressed into
Piece, then film or sweet tablet are carried out, examine, packing, produce tablet.The addition of magnesium stearate is Chinese medicine preparation gross mass
2%.The addition of talcum powder is the 1% of Chinese medicine preparation gross mass.The addition of beta-schardinger dextrin is Chinese medicine preparation gross mass
3%.
Embodiment 2：A kind of preparation method for the dendrobium candidum Chinese medicine preparation for treating osteoporosis
A kind of dendrobium candidum Chinese medicine preparation for treating osteoporosis, by weight, the Chinese medicine preparation includes：Iron sheet stone
42 parts of dry measure used in former times extract solution, 23 parts of tortoiseshell, 21 parts of small lycopod, 16 parts of sheep hinge, 20 parts of the root of herbaceous peony, 18 parts of the root of bidentate achyranthes, 13 parts of subprostrate sophora, the fruit of medicinal cornel
20 parts, 25 parts of barrenwort, 14 parts of shrubby woodfordia root and flower, 14 parts of ginger, 21 parts of radix glycyrrhizae.
The formulation of the dendrobium candidum Chinese medicine preparation of the treatment osteoporosis is tablet.
Preparation method comprises the following steps：
(1) fresh dendrobium candidum is weighed, cleans, cut section, into juice extractor, by several times plus appropriate amount of water is distilled, squeezes the juice, with 200
Mesh filter-cloth filtering, after filtrate is heated to boiling, 4 DEG C of preservations are placed in after being maintained at slight boiling condition lower 4 minutes, obtain squeezeding juice.
(2) squeezeding juice for taking step (1) to obtain, it is 160mg/mL to be diluted with water to solid content, heating and refluxing extraction 3 times,
In heating and refluxing extraction, each micro-boiling 1.1 hours (i.e. squeezeding juice carries out refluxing extraction under slight boiling condition), merge extraction
Liquid, concentrate is obtained with Rotary Evaporators concentrated extracting solution, the water of 2.6 times of weight is added in the most backward concentrate, is placed
Overnight, the filtrate obtained after next day filtering is dendrobium candidum extract solution.
(3) tortoiseshell, small lycopod, sheep hinge and the root of herbaceous peony are taken, the ethanol that the volume fraction for adding 12 times of volumes is 75%, backflow carries
Taking 4 times, 2.2 hours every time, merge ethanol extract, the filtrate being filtrated to get is condensed into the medicinal extract that relative density is 1.32 again,
Relative density is measured when 60 DEG C, and ethanol is reclaimed in concentration process.
(4) ginger and radix glycyrrhizae are taken, adds the water of 16 times of volumes to soak 13 hours, steam distillation 8 hours, obtain condensate liquid and
The dregs of a decoction, the condensate liquid obtain volatile oil and the aqueous solution after water-oil separating.
(5) dregs of a decoction obtained by step (4) are mixed with the root of bidentate achyranthes, subprostrate sophora, the fruit of medicinal cornel, barrenwort and shrubby woodfordia root and flower, and thereto
The decocting for adding 16 times of volumes is boiled 5 times, 2 hours every time, collecting decoction, filtrate is obtained after filtering.
(6) aqueous solution that step (4) is collected is merged with the filtrate obtained by step (5), it is 1.3 to be condensed into relative density
Clear cream, what relative density was surveyed when 60 DEG C.
(7) medicinal extract that clear cream, the step (3) obtained dendrobium candidum extract solution, step (6) obtains merges, vacuum drying
Dry extract is obtained afterwards, the dry extract is crushed, and it is drug extract powder to cross after 80 mesh sieves obtained screenings；Take magnesium stearate, cunning
Volatile oil, the beta-schardinger dextrin that stone flour and said medicine extract powder, step (4) obtain merge, mixed, pelletizing, whole grain, are pressed into
Piece, then film or sweet tablet are carried out, examine, packing, produce tablet.The addition of magnesium stearate is Chinese medicine preparation gross mass
1%.The addition of talcum powder is the 0.5% of Chinese medicine preparation gross mass.The addition of beta-schardinger dextrin is Chinese medicine preparation gross mass
2%.
Embodiment 3：A kind of preparation method for the dendrobium candidum Chinese medicine preparation for treating osteoporosis
A kind of dendrobium candidum Chinese medicine preparation for treating osteoporosis, by weight, the Chinese medicine preparation includes：Iron sheet stone
50 parts of dry measure used in former times extract solution, 26 parts of tortoiseshell, 25 parts of small lycopod, 19 parts of sheep hinge, 24 parts of the root of herbaceous peony, 15 parts of the root of bidentate achyranthes, 18 parts of subprostrate sophora, the fruit of medicinal cornel
26 parts, 30 parts of barrenwort, 18 parts of shrubby woodfordia root and flower, 18 parts of ginger, 24 parts of radix glycyrrhizae.
The formulation of the dendrobium candidum Chinese medicine preparation of the treatment osteoporosis is tablet.
Preparation method comprises the following steps：
(1) fresh dendrobium candidum is weighed, cleans, cut section, into juice extractor, by several times plus appropriate amount of water is distilled, squeezes the juice, with 200
Mesh filter-cloth filtering, after filtrate is heated to boiling, 4 DEG C of preservations are placed in after being maintained at slight boiling condition lower 5 minutes, obtain squeezeding juice.
(2) squeezeding juice for taking step (1) to obtain, it is 200mg/mL to be diluted with water to solid content, heating and refluxing extraction 5 times,
In heating and refluxing extraction, each micro-boiling 1.5 hours (i.e. squeezeding juice carries out refluxing extraction under slight boiling condition), merge extraction
Liquid, concentrate is obtained with Rotary Evaporators concentrated extracting solution, the water of 3 times of weight is added in the most backward concentrate, was placed
At night, the filtrate obtained after next day filtering is dendrobium candidum extract solution.
(3) tortoiseshell, small lycopod, sheep hinge and the root of herbaceous peony are taken, the ethanol that the volume fraction for adding 15 times of volumes is 95%, backflow carries
Take 6 times, 3 hours every time, merge ethanol extract, the filtrate being filtrated to get is condensed into the medicinal extract that relative density is 1.35, phase again
Density is measured in 60 DEG C when, ethanol is reclaimed in concentration process.
(4) ginger and radix glycyrrhizae are taken, adds the water of 20 times of volumes to soak 24 hours, steam distillation 12 hours, obtains condensate liquid
And the dregs of a decoction, the condensate liquid obtain volatile oil and the aqueous solution after water-oil separating.
(5) dregs of a decoction obtained by step (4) are mixed with the root of bidentate achyranthes, subprostrate sophora, the fruit of medicinal cornel, barrenwort and shrubby woodfordia root and flower, and thereto
The decocting for adding 20 times of volumes is boiled 6 times, 3 hours every time, collecting decoction, filtrate is obtained after filtering.
(6) aqueous solution that step (4) is collected is merged with the filtrate obtained by step (5), is condensed into relative density as 1.35
Clear cream, what relative density was surveyed when 60 DEG C.
(7) medicinal extract that clear cream, the step (3) obtained dendrobium candidum extract solution, step (6) obtains merges, vacuum drying
Dry extract is obtained afterwards, the dry extract is crushed, and it is drug extract powder to cross after 100 mesh sieves obtained screenings；Take magnesium stearate, cunning
Volatile oil, the beta-schardinger dextrin that stone flour and said medicine extract powder, step (4) obtain merge, mixed, pelletizing, whole grain, are pressed into
Piece, then film or sweet tablet are carried out, examine, packing, produce tablet.The addition of magnesium stearate is Chinese medicine preparation gross mass
3%.The addition of talcum powder is the 2% of Chinese medicine preparation gross mass.The addition of beta-schardinger dextrin is Chinese medicine preparation gross mass
1%.
The test of pesticide effectiveness is carried out to the Chinese medicine preparation of the present invention based on dendrobium candidum, it is specific as follows：
1st, animal
Kunming mice, SPF levels, 18-22g, female.
2nd, medicine
The Chinese medicine preparation based on dendrobium candidum that embodiment 1~3 prepares.
3rd, positive drug
Estradiol valerate tablet, lot number：27A2, Bayer HealthCare Co.
4th, test method：
Mouse peritoneal injection chloraldurate (dosage 200mg/kg) is anaesthetized, and abdomen position is fixed, and cuts off dorsal midline diamond wool,
It is local that iodine is gone with iodine tincture disinfection and 75% alcohol 1/3rd under trunk, 1cm stringer otch is about near backbone, point
After skin, make two otch at each 1~2cm in both sides, after separating muscle layer, pull out faint yellow cellulite, one can be found
The thin-line-shaped irregular tissue in yellowish pink is ovary；Under to connect thick tubular tissue be cornua uteri, cut off ovary, tissue pushed back
Abdominal cavity, sterilized after suturing muscle layer and skin layer otch, postoperative muscle injection novocillin 120,000 unit/only, conventinal breeding,
For experiment.
Art the latter moon (allows animal to recover state, and eliminates hormone retaining in vivo), be randomly divided into castration model group,
1~3 group of estradiol group, embodiment, totally 5 groups, every group 12, separately take and be only used as sham-operation group with batch mouse 12.Estradiol is administered
Dosage：0.17mg/kg；Chinese medicine preparation dosage based on dendrobium candidum is 0.2mg/kg.Each group gives relative medicine by body weight, false
Operation group and castration model group give isometric distilled water, 1 time a day, continuous 1 month.
Internal organs processing and histopathological examination：After experiment terminates, cervical dislocation puts to death mouse.Uterus is taken, is being divided
Analyse and calculate organ coefficient (organ weights/body weight × ‰), more each group difference on balance after precise weighing.Uterus after weighing
Fixed immediately with 10% formalin, check pathological section is done after HE dyeing.Lesion is divided into level Four by light and heavy degree：Nothing (-),
Gently (+), in (++), again (+++), be calculated as 0,1,2,3 point respectively, pathological observation project be epithelial lesion, body of gland change, inflammation it is anti-
Should, fibroplasia and blood vessel hyperplasia phenomenon etc. 5.
5th, experimental result
(1) bone trabecula pathological examination results
Sham-operation group bone trabecula is sturdy, full, morphosis is complete, and coloring is deeper, and marshalling simultaneously connects networking, gap
Smaller, trabecular bone density, area are normal, and ossis is relatively small, and it is more that intracavitary makes erythrocyte number.Castration model group bone trabecula
Attenuate, cortex of bone is thinning, and morphosis is poor, there is distortion and fracture, gap increase, and bone density, area are reduced, and intracavitary is made red thin
Born of the same parents' quantity is reduced, and fat vacuole is more, in typical osteoporosis lesion, illustrates modeling success.Compared with model group, estradiol group
Bone trabecula is slightly thick, and arrangement is still neat and connects networking, and subregion bone trabecula gap slightly increases, and trabecular bone density, area are without bright
Aobvious to reduce, hematopoietic cells number is more in pulp cavity；Chinese medicine preparation group trabecular connectivity of the present invention is preferable, and cortex of bone is without bright
Aobvious thinning, bone trabecula is without substantially attenuating, and subregion bone trabecula gap slightly increases, and area is without significantly reducing, and hematopoiesis is thin in pulp cavity
Born of the same parents' quantity is more.
Embodiment 4：A kind of preparation method for the dendrobium candidum Chinese medicine preparation for treating osteoporosis
A kind of preparation method for the dendrobium candidum Chinese medicine preparation for treating osteoporosis, the iron sheet stone of the treatment osteoporosis
The composition of raw materials of dry measure used in former times Chinese medicine preparation includes the component of following parts by weight：
Preparation method comprises the following steps：
The first step：Squeezed the juice after fresh dendrobium candidum is mixed with water, then with 200 mesh filtered through gauze, obtained filtrate is micro-
Boiling preserves under the conditions of being placed in 4 DEG C after 3 minutes, obtains squeezeding juice；
Second step：It is 200mg/mL that the squeezeding juice is diluted with water into solid content, then heating and refluxing extraction 2 times,
During heating and refluxing extraction, each micro-boiling 1.5 hours, merge extract solution, next concentrating the extract solution with Rotary Evaporators obtains
To concentrate, the water of 3 times of weight is most added in the concentrate backward, is stood overnight, the filtrate obtained after next day filtering is iron
Skin dendrobium extract；
3rd step：Tortoiseshell, small lycopod, sheep hinge and the root of herbaceous peony are taken, the ethanol that the volume fraction for adding 8 times of volumes is 60%, is returned
Stream extraction 3 times, 1.5 hours every time, merge ethanol extract, it is 1.35 that the filtrate obtained after filtering is condensed into relative density again
Medicinal extract, relative density measure when 60 DEG C；
4th step：Ginger and radix glycyrrhizae are taken, adds the water of 7 times of volumes to soak 15 hours, steam distillation 2 hours, is condensed
Liquid and the dregs of a decoction, the condensate liquid obtain volatile oil and the aqueous solution after water-oil separating；
5th step：The dregs of a decoction obtained by 4th step are mixed with the root of bidentate achyranthes, subprostrate sophora, the fruit of medicinal cornel, barrenwort, shrubby woodfordia root and flower, and to its
The middle decocting for adding 13 times of volumes is boiled 5 times, and 1.5 hours every time, filtrate is obtained after collecting decoction filtering；
6th step：The aqueous solution collected by 4th step is merged with the filtrate obtained by the 5th step, being condensed into relative density is
1.25 clear cream, relative density measure when 60 DEG C；
7th step：Dendrobium candidum extract solution, medicinal extract are merged with clear cream, dry extract is obtained after vacuum drying, by the dry leaching
Cream crushes, and it is drug extract powder to cross after 100 mesh sieves obtained screenings；By magnesium stearate, talcum powder, the drug extract powder,
The volatile oil that 4th step obtains merges with beta-schardinger dextrin, mixed, pelletizing, whole grain, tabletted, then after carrying out film or sweet tablet
Produce the dendrobium candidum Chinese medicine preparation for the treatment of osteoporosis.The addition of magnesium stearate is the 3% of Chinese medicine preparation gross mass.Talcum
The addition of powder is the 2% of Chinese medicine preparation gross mass.The addition of beta-schardinger dextrin is the 1% of Chinese medicine preparation gross mass.
As described above is only to explain the preferred embodiments of the invention, is not intended to tool to do any shape to the present invention
Limitation in formula, therefore all have any modification or change for making the relevant present invention under identical spirit, it should all wrap
Include the invention is intended to the category protected.
Claims (3)
- A kind of 1. preparation method for the dendrobium candidum Chinese medicine preparation for treating osteoporosis, it is characterised in that：The treatment sclerotin is dredged The composition of raw materials of the dendrobium candidum Chinese medicine preparation of pine includes the component of following parts by weight：26~50 parts of dendrobium candidum extract solution；12~26 parts of tortoiseshell；Small 12~25 parts of lycopod；9~19 parts of sheep hinge；8~24 parts of the root of herbaceous peony；7~15 parts of the root of bidentate achyranthes；5~18 parts of subprostrate sophora；12~26 parts of the fruit of medicinal cornel；13~30 parts of barrenwort；10~18 parts of shrubby woodfordia root and flower；7~18 parts of ginger；12~24 parts of radix glycyrrhizae；Preparation method comprises the following steps：The first step：Squeezed the juice after fresh dendrobium candidum is mixed with water, then with 200 mesh filtered through gauze, obtained filtrate micro-boiling 3~ Preserved under the conditions of 4 DEG C are placed in after 5 minutes, obtain squeezeding juice；Second step：It is 50~200mg/mL that the squeezeding juice is diluted with water into solid content, then heating and refluxing extraction 2 ~ 5 times, In heating and refluxing extraction, each micro-boiling 0.5~1.5 hour, merge extract solution, next carried described in Rotary Evaporators concentrate Take liquid to obtain concentrate, the water of 1.5~3 times of weight is added in the most backward concentrate, is stood overnight, is obtained after next day filtering Filtrate be dendrobium candidum extract solution；3rd step：Tortoiseshell, small lycopod, sheep hinge and the root of herbaceous peony are taken, the ethanol that the volume fraction for adding 3~15 times of volumes is 50~95%, Refluxing extraction 1~6 time, 0.5~3 hour every time, merge ethanol extract, the filtrate obtained after filtering is condensed into relative density again For 1.25~1.35 medicinal extract, relative density measures when 60 DEG C；4th step：Ginger and radix glycyrrhizae are taken, adds the water of 5~20 times of volumes to soak 0.5~24 hour, steam distillation 0.5~12 is small When, condensate liquid and the dregs of a decoction are obtained, the condensate liquid obtains volatile oil and the aqueous solution after water-oil separating；5th step：The dregs of a decoction obtained by 4th step are mixed with the root of bidentate achyranthes, subprostrate sophora, the fruit of medicinal cornel, barrenwort, shrubby woodfordia root and flower, and added thereto The decocting for entering 5~20 times of volumes is boiled 1~6 time, and 1~3 hour every time, filtrate is obtained after collecting decoction filtering；6th step：The aqueous solution collected by 4th step is merged with the filtrate obtained by the 5th step, is condensed into relative density as 1.25 ~1.35 clear cream, relative density measure when 60 DEG C；7th step：Dendrobium candidum extract solution, medicinal extract are merged with clear cream, dry extract is obtained after vacuum drying, by the dry extract Broken, it is drug extract powder to cross after 60~100 mesh sieves obtained screenings；By magnesium stearate, talcum powder, the drug extract powder, The volatile oil that 4th step obtains merges with beta-schardinger dextrin, mixed, pelletizing, whole grain, tabletted, then after carrying out film or sweet tablet Produce the dendrobium candidum Chinese medicine preparation for the treatment of osteoporosis.
- 2. the preparation method of the dendrobium candidum Chinese medicine preparation for the treatment of osteoporosis according to claim 1, it is characterised in that： The composition of raw materials of the dendrobium candidum Chinese medicine preparation of the treatment osteoporosis includes the component of following parts by weight：26~50 parts of dendrobium candidum extract solution；12~26 parts of tortoiseshell；Small 12~25 parts of lycopod；9~19 parts of sheep hinge；8~24 parts of the root of herbaceous peony；7~15 parts of the root of bidentate achyranthes；5~18 parts of subprostrate sophora；12~26 parts of the fruit of medicinal cornel；13~30 parts of barrenwort；10~18 parts of shrubby woodfordia root and flower；7~18 parts of ginger；12~24 parts of radix glycyrrhizae.
- 3. the preparation method of the dendrobium candidum Chinese medicine preparation for the treatment of osteoporosis according to claim 1, it is characterised in that： Preparation method comprises the following steps：The first step：Squeezed the juice after fresh dendrobium candidum is mixed with water, then with 200 mesh filtered through gauze, obtained filtrate micro-boiling 4 is divided Preserved under the conditions of 4 DEG C are placed in after clock, obtain squeezeding juice；Second step：It is 160mg/mL that the squeezeding juice is diluted with water into solid content, then heating and refluxing extraction 3 times, is being heated During refluxing extraction, each micro-boiling 1.1 hours, merge extract solution, next obtained with the Rotary Evaporators concentration extract solution dense Contracting liquid, the water of 2.6 times of weight is most added in the concentrate backward, is stood overnight, the filtrate obtained after next day filtering is iron sheet Dendrobium extract；3rd step：Tortoiseshell, small lycopod, sheep hinge and the root of herbaceous peony are taken, the ethanol that the volume fraction for adding 12 times of volumes is 75%, backflow carries Take 4 times, 2.2 hours every time, merge ethanol extract, the filtrate obtained after filtering is condensed into the leaching that relative density is 1.32 again Cream, relative density measure when 60 DEG C；4th step：Take ginger and radix glycyrrhizae, add the water of 16 times of volumes to soak 13 hours, steam distillation 8 hours, obtain condensate liquid and The dregs of a decoction, the condensate liquid obtain volatile oil and the aqueous solution after water-oil separating；5th step：The dregs of a decoction obtained by 4th step are mixed with the root of bidentate achyranthes, subprostrate sophora, the fruit of medicinal cornel, barrenwort, shrubby woodfordia root and flower, and added thereto The decocting for entering 16 times of volumes is boiled 5 times, and 2 hours every time, filtrate is obtained after collecting decoction filtering；6th step：The aqueous solution collected by 4th step is merged with the filtrate obtained by the 5th step, it is 1.3 to be condensed into relative density Clear cream, relative density measure when 60 DEG C；7th step：Dendrobium candidum extract solution, medicinal extract are merged with clear cream, dry extract is obtained after vacuum drying, by the dry extract Broken, it is drug extract powder to cross after 80 mesh sieves obtained screenings；By magnesium stearate, talcum powder, the drug extract powder, the 4th step Obtained volatile oil merges with beta-schardinger dextrin, mixed, pelletizing, whole grain, tabletted, then produce after film or sweet tablet controlling Treat the dendrobium candidum Chinese medicine preparation of osteoporosis.
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Application publication date: 20171222 |