CN108219025A - A kind of extracting method of the high dendrobium candidum of recovery rate - Google Patents
A kind of extracting method of the high dendrobium candidum of recovery rate Download PDFInfo
- Publication number
- CN108219025A CN108219025A CN201810048760.8A CN201810048760A CN108219025A CN 108219025 A CN108219025 A CN 108219025A CN 201810048760 A CN201810048760 A CN 201810048760A CN 108219025 A CN108219025 A CN 108219025A
- Authority
- CN
- China
- Prior art keywords
- dendrobium candidum
- extraction
- recovery rate
- extracting method
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of extracting methods of the high dendrobium candidum of recovery rate, 1. just extract：Fresh stem of Dendrobium is taken, is crushed, crosses 10 20 mesh sieve, adds 68 times of amount water, stirring dissipates, 20 30min are decocted, are filtered while hot, filter residue repeats to decoct 23 times, merging filtrate, and 24 48h are freezed under the conditions of 25 20 DEG C of postposition of bottling, it is freeze-dried again, obtains dried extract, crushed, obtain A product；2. second extraction：A product are placed in heating and refluxing extraction in thermostat water bath, filter while hot, and filter residue repeats extraction 34 times, and merging filtrate, relative density is 1.1 1.2 thick paste when filtrate is condensed into 50 60 DEG C, and thick paste drying to constant weight is to get dry cream extract.When present invention process extracts dendrobium candidum, polysaccharide extract rate is up to 42%, promotes the utilization to Dendrobium officinale polysaccharide.
Description
Technical field
The present invention relates to a kind of extracting method of dendrobium candidum, the high dendrobium candidum of particularly a kind of polysaccharide extract rate carries
Take method.
Background technology
Dendrobium candidum nickname iron sheet orchid, bracketplant, Yunnan iron sheet, iron sheet bracketplant are orchid family Dendrobium herbaceos perennials,
Dendrobium candidum is the top grade of Shihu " medicinal materials, is known as the laudatory title of " gold in Chinese celestial grass-medicine ", is used as medicine with stem, contains polysaccharide, stone
The active ingredients such as dry measure used in former times alkali, alkaloid, with nourishing Yin and clearing heat, the beneficial stomach that promotes the production of body fluid, wet one's whistle improving eyesight and other effects, in antitumor, raising human body
Immunity, anti-aging, anti-oxidant, platelet aggregation-against, reduces blood glucose, treatment cataract etc. at treatment enterogastric diseases
There is good efficacy.
Modern pharmacology research shows that dendrobium candidum has humidification to non-specific and specific immunity, anti-swollen
Knurl, treatment that is anti-oxidant, reducing blood glucose and cataract etc., pharmacological action is notable.The drug action of dendrobium candidum is main
It is because it contains a variety of active ingredients, such as alkaloid, polysaccharide, amino acid, but phase not to the utmost the effect of various active constituents
Together.And dendrobium candidum clinically mainly by the way that dendrobium candidum medicinal material tanning decoction of medicinal ingredients is taken worked by application, each active constituent
The effect of do not applied targetedly, so the utilization of dendrobium candidum mainly also rests on the primary stage of low side, medicine
It also needs further to develop with value.Research shows that polysaccharide content of dendrobium candidum is higher, the antioxidation of polysaccharide is apparent, has good
Good potential Development volue.If recycled after its polysaccharide is extracted has important meaning to the further exploitation of dendrobium candidum
Justice.
There is hot-water process to the extracting method of Dendrobium officinale polysaccharide at present, it is enzyme-linked to close extraction method and supercritical ultrasonics technology etc..With top
Method has their own advantages, but generally speaking various methods also have certain limitation, and polysaccharide extract rate is low, generally 30% hereinafter, limitation
The utilization of Dendrobium officinale polysaccharide active constituent.
Invention content
The purpose of the present invention, is to provide a kind of extraction process of dendrobium candidum, and present invention process carries dendrobium candidum
When taking, polysaccharide extract rate is high, promotes the utilization to Dendrobium officinale polysaccharide.
The invention is realized in this way：1. a kind of extracting method of the high dendrobium candidum of recovery rate is just extracted：Take fresh stone
Dry measure used in former times stem crushes, and crosses 10-20 mesh sieve, adds 6-8 times to measure water, and stirring dissipates, and decocts 20-30min, filters while hot, and filter residue repeats to decoct
2-3 times, merging filtrate, bottle -25--20 DEG C of postposition under the conditions of freeze 24-48h, then be freeze-dried, obtain dried extract,
It crushes, obtains A product；
2. second extraction：A product are placed in heating and refluxing extraction in thermostat water bath, filter while hot, and filter residue repeats extraction 3-4 times, close
And filtrate, relative density is the thick paste of 1.1-1.2 when filtrate is condensed into 50-60 DEG C, and thick paste drying to constant weight is extracted to get dry cream
Object.
In the extracting method of the high dendrobium candidum of aforementioned recovery rate, 1. step is；Fresh stem of Dendrobium is taken, is crushed, crosses 10-
20 mesh sieve, and add 7 times of amount water, and stirring dissipates, decocts 25min, filter while hot, and filter residue repeats to decoct 3 times, merging filtrate, after bottling
36h is freezed under the conditions of putting -25--20 DEG C, then is freeze-dried, obtains dried extract, crushes, obtains A product；
In the extracting method of the high dendrobium candidum of aforementioned recovery rate, step 2. in, during heating and refluxing extraction, Extracting temperature 80
℃。
In the extracting method of the high dendrobium candidum of aforementioned recovery rate, step 2. in, during heating and refluxing extraction, extraction time
For 90-120min.
In the extracting method of the high dendrobium candidum of aforementioned recovery rate, step 2. in, during heating and refluxing extraction, solid-liquid ratio is
1:35-45。
In the extracting method of the high dendrobium candidum of aforementioned recovery rate, the solid-liquid ratio is 1:40.
Applicant has carried out a large amount of experimental study to the present invention, and part Experiment is as follows：
Experimental example single factor exploration
When applicant is to second extraction, the parameter of heating and refluxing extraction has carried out a large amount of research, is specifically shown in Table 1.
It is obtained by table：
1. influence of the Extracting temperature to Dendrobium officinale polysaccharide recovery rate:Polysaccharide extract rate highest at 80 DEG C, under hereafter gradual
Drop.Temperature is excessively high saccharide portion to be caused to decompose, therefore optimum extraction temperature is 80 DEG C.
2. influence of the extraction time to Dendrobium officinale polysaccharide recovery rate:Polysaccharide extract rate is gradual with extraction time extension
It improves, reaches highest in 90-120min, the optimum extraction time is 90min.
3. influence of the solid-liquid ratio to Dendrobium officinale polysaccharide recovery rate:Polysaccharide extract rate is with increasing for solvent dosage and gradual
It improves, solid-liquid ratio is 1:Polysaccharide extract rate highest when 40.Therefore best solid-liquid ratio is 1:40.
4. influence of the extraction time to Dendrobium officinale polysaccharide recovery rate:Polysaccharide extract rate increases and gradual with extraction time
It improves, recovery rate highest when extracting 4 times.Therefore optimum extraction number is 4 times.
2 purity of polysaccharide of embodiment is investigated
1 material
3 parts of the dendrobium candidum that weight is identical is taken, extract 1, extract 2 and extract 3 is made as follows respectively.
Extract 1：It is extracted by embodiment 1.
Extract 2：Iron sheet stone adds water to cook 4 times, each 35min, and decoction liquor merges, and filtering, extracting solution is concentrated into 50-60
DEG C when relative density be 1.1-1.2 thick paste, thick paste drying to constant weight at 50-60 DEG C to get.
Extract 3：Dendrobium candidum adds 7 times of amount ethyl alcohol to extract 3 times, each 2h, and extracting solution merges, filtering, extracting solution concentration
To relative density at 50-60 DEG C be 1.1-1.2 thick paste, thick paste drying to constant weight at 50-60 DEG C to get.
Instrument and reagent
Dextrose standard sample [D mono- (+) glucose]：KAYON companies；Methanol (chromatographically pure)：Close europeanized learn of Tianjin section is tried
Agent Co., Ltd；Remaining reagent is that analysis is pure；Phenol：Shanghai Ling Feng chemical reagent Co., Ltd；Sulfuric acid：Shanghai Ling Feng chemistry
Reagent Co., Ltd.
2 methods
The preparation of 2.1 test solutions
2.1.1 the preparation of test solution 1：
Extract 1 is taken, is crushed, No. three sieves is crossed, takes powder 1.5g after filtering, volatilize solvent, be transferred in 250 mL measuring bottles, add
Water is shaken up to scale, is filtered, and precision measures 2 mL of subsequent filtrate, puts in 15 mL centrifuge tubes, and precision adds in 95% ethyl alcohol, shakes up,
Refrigeration 1 hour is taken out, and is centrifuged 20 minutes, discards supernatant liquid, and precipitation heating water dissolution is transferred in 25 mL measuring bottles, lets cool,
Scale is added water to, is shaken up to get test solution 1.
2.1.2 the preparation of test solution 2
Extract 2 is taken, is crushed, No. three sieves is crossed, takes powder 1.5g after filtering, volatilize solvent, be transferred in 250 mL measuring bottles, add
Water is shaken up to scale, is filtered, and precision measures 2 mL of subsequent filtrate, puts in 15 mL centrifuge tubes, and precision adds in 95% ethyl alcohol, shakes up,
Refrigeration 1 hour is taken out, and is centrifuged 20 minutes, discards supernatant liquid, and precipitation heating water dissolution is transferred in 25 mL measuring bottles, lets cool,
Scale is added water to, is shaken up to get test solution 2.
2.1.3 the preparation of test solution
Extract 3 is taken, is crushed, No. three sieves is crossed, takes powder 1.5g after filtering, volatilize solvent, be transferred in 250 mL measuring bottles, add water
It to scale, shakes up, filters, precision measures 2 mL of subsequent filtrate, puts in 15 mL centrifuge tubes, and precision adds in 95% ethyl alcohol, shakes up, cold
It hides 1 hour, takes out, centrifuge 20 minutes, discard supernatant liquid, precipitation heating water dissolution is transferred in 25 mL measuring bottles, lets cool, add
Water is shaken up to scale to get test solution 3.
2.2 the preparation of reference substance solution
The DEXTROSE ANHYDROUS that precision weighs 105 DEG C of dryings to constant weights is appropriate, adds water that 99 mg/L solution are made, spare.
It is prepared by 2.3 standard curves
Precision measures 0.2,0.4,0.6,0.8,1.0 m L of reference substance solution and puts in 10m L tool plug test tubes, adds water to
1.0mL, precision add in 5% phenol solution 1mL (I is now prepared before use), shake up, then accurate addition 5 m L of sulfuric acid, shake up, put
It is heated 20 minutes in boiling water bath, takes out, be put into ice-water bath and cool down 5 minutes, using corresponding reagent as blank, in 488 nm wavelength
Place measures absorbance, and using absorbency Y as ordinate, concentration X (g/L) is abscissa, draws standard curve, and regression equation is：Y
=11.772X -0.1492, r=0.9995.The result shows that polysaccharide linear relationship in the range of 0.02-0.10 g/L is good
It is good.
2.4 measuring method
It is accurate respectively to measure 3 each 0.5 mL of test solution 1, test solution 2 and test solution, put 10 mL tool plug test tubes
In, 1mL is added water to, by the method under the preparation of " 2.3 " standard curve, from " precision adds in 5% phenol solution 1m L ",
Measure absorbance in accordance with the law, from standard curve read test solution in DEXTROSE ANHYDROUS content, calculate to get.
2.5 precision test
Precision draws 6 parts, every part of 0.6 m L of glucose control product solution, and absorbance is measured according to the method under " 2.4 " item.It surveys
Determine as a result, RSD is respectively 1.60% and 1.62%, precision is good.
2.6 repetitive test
6 parts of test solutions 1, test solution 2 and test solution 3 are prepared as stated above, are inhaled according to measuring as stated above
Luminosity.Measurement result, RSD are respectively 2.70% and 2.71%, and repeatability is good.
2.7 stability test
Take same test solution 1, test solution 2 and test solution 3 respectively, place at room temperature, respectively at 10min,
After 30min, 1 h, 2 h, 4 h, 6 h as stated above, test solution 2 and test solution 3 stablize in 6 hours.
2.8 sample recovery rates are tested
6 parts, every part of about 1.65g of 1 powder of extract is weighed, it is accurately weighed.Accurate addition glucose control product solution is (a concentration of
0.099 g/L) 2 mL, test solution 1 is prepared as stated above, is measured as stated above, is calculated polyoses content, is as a result put down
The equal rate of recovery is 94.9%, RSD 2.7%.
6 parts, every part of about 1.65g of 2 powder of extract is weighed, it is accurately weighed.Accurate addition glucose control product solution is (dense
Spend for 0.099 g/L) 2 mL, test solution 2 is prepared as stated above, is measured as stated above, calculates polyoses content, knot
Fruit average recovery rate is 93.9%, RSD 2.6%.
6 parts, every part of about 1.65g of 3 powder of extract is weighed, it is accurately weighed.Accurate addition glucose control product solution is (dense
Spend for 0.099 g/L) 2 mL, test solution 3 is prepared as stated above, is measured as stated above, calculates polyoses content, knot
Fruit average recovery rate is 93.8%, RSD 2.6%.
2.9 samples measure
By the preparation method and determination condition of above-mentioned test solution 1, test solution 2 and test solution 3, measure 6 parts and carry
Take the content of polysaccharide in 1 sample of object and 6 parts of 2 samples of dendrobium candidum, record the results are shown in Table 1 as a result, results are averaged.
As seen from table, present invention process to dendrobium candidum into during extraction, polysaccharide containing 889.23g in every gram of extract, polysaccharide
Purity is 88.9%, and the purity of polysaccharide in extract 2 and extract 3 is respectively 64.4% and 64.0%.
Compared with prior art, when present invention process extracts dendrobium candidum, recovery rate is high, and purity of polysaccharide is up to
88.9%。
Specific embodiment
Embodiment 1.
A kind of extracting method of the high dendrobium candidum of recovery rate, including：
1. it just extracts：Fresh stem of Dendrobium is taken, is crushed, crosses 10-20 mesh sieve, adds 7 times of amount water, stirring dissipates, and decocts 25min, while hot
Filtering, filter residue repeat to decoct 3 times, merging filtrate, and 36h is freezed, then be freeze-dried under the conditions of -25--20 DEG C of postposition of bottling,
Dried extract is obtained, crushes, obtains A product；
2. second extraction：A product are placed in heating and refluxing extraction in thermostat water bath, filter while hot, and filter residue repeats extraction 4 times, merge
Filtrate, relative density is the thick paste of 1.1-1.2 when filtrate is condensed into 50-60 DEG C, and thick paste drying to constant weight is extracted to get dry cream
Object.
Step 2. in, during heating and refluxing extraction, Extracting temperature is 80 DEG C；Extraction time is 90min；Solid-liquid ratio is 1:40.
Embodiment 2.
A kind of extracting method of the high dendrobium candidum of recovery rate, including：
1. it just extracts：Fresh stem of Dendrobium is taken, is crushed, crosses 10-20 mesh sieve, adds 8 times of amount water, stirring dissipates, and decocts 30min, while hot
Filtering, filter residue repeat to decoct 3 times, merging filtrate, and 48h is freezed, then be freeze-dried under the conditions of -25--20 DEG C of postposition of bottling,
Dried extract is obtained, crushes, obtains A product；
2. second extraction：A product are placed in heating and refluxing extraction in thermostat water bath, filter while hot, and filter residue repeats extraction 4 times, merge
Filtrate, relative density is the thick paste of 1.1-1.2 when filtrate is condensed into 50-60 DEG C, and thick paste drying to constant weight is extracted to get dry cream
Object.
Step 2. in, during heating and refluxing extraction, Extracting temperature is 80 DEG C；Extraction time is 120min；Solid-liquid ratio is 1:35.
Embodiment 3.
A kind of extracting method of the high dendrobium candidum of recovery rate, including：
1. it just extracts：Fresh stem of Dendrobium is taken, is crushed, crosses 10-20 mesh sieve, adds 6 times of amount water, stirring dissipates, and decocts 20min, while hot
Filtering, filter residue repeat to decoct 2 times, merging filtrate, are freezed for 24 hours, then be freeze-dried under the conditions of -25--20 DEG C of postposition of bottling,
Dried extract is obtained, crushes, obtains A product；
2. second extraction：A product are placed in heating and refluxing extraction in thermostat water bath, filter while hot, and filter residue repeats extraction 3 times, merge
Filtrate, relative density is the thick paste of 1.1-1.2 when filtrate is condensed into 50-60 DEG C, and thick paste drying to constant weight is extracted to get dry cream
Object.
Step 2. in, during heating and refluxing extraction, Extracting temperature is 80 DEG C；Extraction time is 90min；Solid-liquid ratio is 1:45.
Claims (5)
1. a kind of extracting method of the high dendrobium candidum of recovery rate, it is characterised in that：
1. it just extracts：Fresh stem of Dendrobium is taken, is crushed, crosses 10-20 mesh sieve, adds 6-8 times to measure water, stirring dissipates, and decocts 20-30min,
It filters while hot, filter residue repeats to decoct 2-3 times, merging filtrate, and 24-48h is freezed, then carry out under the conditions of -25--20 DEG C of postposition of bottling
Freeze-drying, obtains dried extract, crushes, obtains A product；
2. second extraction：A product are placed in heating and refluxing extraction in thermostat water bath, filter while hot, and filter residue repeats extraction 3-4 times, close
And filtrate, relative density is the thick paste of 1.1-1.2 when filtrate is condensed into 50-60 DEG C, and thick paste drying to constant weight is extracted to get dry cream
Object.
2. the extracting method of the high dendrobium candidum of recovery rate as described in claim 1, it is characterised in that：1. step is；It takes new
Fresh stem of Dendrobium crushes, and crosses 10-20 mesh sieve, adds 7 times of amount water, and stirring dissipates, decocts 25min, filter while hot, and filter residue repeats to decoct 3
It is secondary, merging filtrate, bottle -25--20 DEG C of postposition under the conditions of freeze 36h, then be freeze-dried, obtain dried extract, crush,
Obtain A product；
The extracting method of the high dendrobium candidum of recovery rate as described in claim 1, it is characterised in that：Step 2. in, heat back
During stream extraction, Extracting temperature is 80 DEG C.
3. the extracting method of the high dendrobium candidum of recovery rate as described in claim 1, it is characterised in that：Step 2. in, heating
During refluxing extraction, extraction time 90-120min.
4. the extracting method of the high dendrobium candidum of recovery rate as described in claim 1, it is characterised in that：Step 2. in, heating
During refluxing extraction, solid-liquid ratio 1:35-45.
5. the extracting method of the high dendrobium candidum of recovery rate as claimed in claim 5, it is characterised in that：The solid-liquid ratio is
1:40。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810048760.8A CN108219025A (en) | 2018-01-18 | 2018-01-18 | A kind of extracting method of the high dendrobium candidum of recovery rate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810048760.8A CN108219025A (en) | 2018-01-18 | 2018-01-18 | A kind of extracting method of the high dendrobium candidum of recovery rate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108219025A true CN108219025A (en) | 2018-06-29 |
Family
ID=62667879
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810048760.8A Withdrawn CN108219025A (en) | 2018-01-18 | 2018-01-18 | A kind of extracting method of the high dendrobium candidum of recovery rate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108219025A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108794650A (en) * | 2018-08-20 | 2018-11-13 | 云南和瑾科技有限公司 | A kind of dendrobium polysaccharide extracting method |
| CN111264736A (en) * | 2020-03-13 | 2020-06-12 | 云南植虫药生物科技有限公司 | Instant solid beverage containing rhizoma polygonati and dendrobium |
| CN111825779A (en) * | 2020-08-21 | 2020-10-27 | 武汉理工大学 | Extraction method of dendrobium officinale polysaccharide |
| CN113577001A (en) * | 2021-09-10 | 2021-11-02 | 天津捷昂康生物科技发展有限公司 | Fresh dendrobium antioxidant composition, preparation method and application of antioxidant |
| CN114469832A (en) * | 2022-03-28 | 2022-05-13 | 上海家化联合股份有限公司 | Dendrobium officinale extract and application thereof |
| CN114605565A (en) * | 2022-02-17 | 2022-06-10 | 合肥卡迪尔生物科技有限公司 | Dendrobium officinale polysaccharide and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101015649A (en) * | 2006-11-23 | 2007-08-15 | 天津市轩宏医药技术有限公司 | Dendrobium candidum polysaccharide extractive, medicine composition thereof and its preparation and use |
| CN104448013A (en) * | 2014-10-27 | 2015-03-25 | 霍山县天下泽雨生物科技发展有限公司 | Process for extracting dendrobium polysaccharide and dendrobine from dendrobium officinale |
| CN104892783A (en) * | 2015-05-11 | 2015-09-09 | 南昌大学 | Purification method of dendrobium officinale polysaccharide |
| CN105504081A (en) * | 2015-12-15 | 2016-04-20 | 云南民族大学 | Method for increasing leaching rate of polysaccharide components in dendrobium candidum |
| CN107251728A (en) * | 2017-05-09 | 2017-10-17 | 贵州济生农业科技有限公司 | A kind of extracting method of dendrobium candidum |
-
2018
- 2018-01-18 CN CN201810048760.8A patent/CN108219025A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101015649A (en) * | 2006-11-23 | 2007-08-15 | 天津市轩宏医药技术有限公司 | Dendrobium candidum polysaccharide extractive, medicine composition thereof and its preparation and use |
| CN104448013A (en) * | 2014-10-27 | 2015-03-25 | 霍山县天下泽雨生物科技发展有限公司 | Process for extracting dendrobium polysaccharide and dendrobine from dendrobium officinale |
| CN104892783A (en) * | 2015-05-11 | 2015-09-09 | 南昌大学 | Purification method of dendrobium officinale polysaccharide |
| CN105504081A (en) * | 2015-12-15 | 2016-04-20 | 云南民族大学 | Method for increasing leaching rate of polysaccharide components in dendrobium candidum |
| CN107251728A (en) * | 2017-05-09 | 2017-10-17 | 贵州济生农业科技有限公司 | A kind of extracting method of dendrobium candidum |
Non-Patent Citations (1)
| Title |
|---|
| 王建明等: "铁皮石斛多糖的提取工艺优化及其抗氧化活性研究", 《西北农林科技大学学报（自然科学版）》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108794650A (en) * | 2018-08-20 | 2018-11-13 | 云南和瑾科技有限公司 | A kind of dendrobium polysaccharide extracting method |
| CN111264736A (en) * | 2020-03-13 | 2020-06-12 | 云南植虫药生物科技有限公司 | Instant solid beverage containing rhizoma polygonati and dendrobium |
| CN111825779A (en) * | 2020-08-21 | 2020-10-27 | 武汉理工大学 | Extraction method of dendrobium officinale polysaccharide |
| CN113577001A (en) * | 2021-09-10 | 2021-11-02 | 天津捷昂康生物科技发展有限公司 | Fresh dendrobium antioxidant composition, preparation method and application of antioxidant |
| CN113577001B (en) * | 2021-09-10 | 2024-04-19 | 天津捷昂康生物科技发展有限公司 | Fresh dendrobium anti-oxidation composition, preparation method and application of anti-oxidation compound |
| CN114605565A (en) * | 2022-02-17 | 2022-06-10 | 合肥卡迪尔生物科技有限公司 | Dendrobium officinale polysaccharide and preparation method and application thereof |
| CN114605565B (en) * | 2022-02-17 | 2023-10-20 | 合肥卡迪尔生物科技有限公司 | Dendrobium candidum polysaccharide and preparation method and application thereof |
| CN114469832A (en) * | 2022-03-28 | 2022-05-13 | 上海家化联合股份有限公司 | Dendrobium officinale extract and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108219025A (en) | A kind of extracting method of the high dendrobium candidum of recovery rate | |
| CN108084291A (en) | The method that polysaccharide is extracted in dendrobium candidum | |
| CN102716367A (en) | Compound dendrobium candidum buccal tablet and preparation method of compound dendrobium candidum buccal tablet | |
| CN108783155A (en) | A kind of sealwort, mulberry leaf, radix polygonati officinalis compatibility beverage and preparation method thereof | |
| CN110025650A (en) | The content assaying method of gypenosides in the preparation method and Herb Gynostemmae Pentaphylli extract of a kind of Herb Gynostemmae Pentaphylli extract | |
| CN107251728A (en) | A kind of extracting method of dendrobium candidum | |
| CN104146256A (en) | Dendrobium stem health-care oral liquid capable of nourishing stomach and preparation method thereof | |
| CN106214845A (en) | A kind of pharmaceutical composition with health care and preparation method thereof | |
| CN108079198A (en) | A kind of extraction process of dendrobium candidum | |
| CN1907481B (en) | Medicinal composition for treating summer heat-dampness rheum and preparation method thereof | |
| CN113777183A (en) | Method for constructing characteristic spectrum of glossy privet fruit medicinal material and processed product thereof and method for detecting content of multi-index components | |
| CN112516219B (en) | Preparation process of traditional Chinese medicine granules for treating systemic lupus erythematosus | |
| CN108456258B (en) | Preparation method of dendrobium officinale selenium polysaccharide | |
| CN102266503A (en) | Sugar-free anemopyretic cold granules and preparation method thereof | |
| CN110658264A (en) | Quality detection method of nine-process sealwort | |
| CN108114255A (en) | A kind of traditional Chinese medicinal ointment grows preparation process | |
| CN109394806B (en) | Processing method of ginseng and polygonatum odoratum | |
| CN108132324A (en) | The TLC Identification of ginseng component in a kind of cantharides capsule | |
| CN112461948A (en) | Method for determining polysaccharide components and monosaccharide compositions in saw palmetto fruits | |
| CN100531774C (en) | A Chinese medicinal composition for treating cough and preparation method thereof | |
| CN102153666B (en) | Sipunculidae polysaccharide and preparation method thereof and application thereof to anti-fatigue function | |
| CN111558008A (en) | Lotus-atractylodes-rhizome fat-reducing navel patch, preparation method and application | |
| CN108314746B (en) | Dendrobium officinale selenium polysaccharide | |
| CN108310119A (en) | A kind of Herba Cistanches chewable tablets and preparation method thereof | |
| CN103446284A (en) | Traditional Chinese medicine compound granules for treating infantile asthma and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180629 |