CN113577001A

CN113577001A - Fresh dendrobium antioxidant composition, preparation method and application of antioxidant

Info

Publication number: CN113577001A
Application number: CN202111058889.5A
Authority: CN
Inventors: 冯金磊
Original assignee: Tianjin Jieangkang Biotechnology Development Co ltd
Current assignee: Tianjin Jieangkang Biotechnology Development Co ltd
Priority date: 2021-09-10
Filing date: 2021-09-10
Publication date: 2021-11-02
Anticipated expiration: 2041-09-10
Also published as: CN113577001B

Abstract

The invention relates to a fresh dendrobe antioxidant composition, an antioxidant preparation method and application, wherein the fresh dendrobe antioxidant composition comprises 20-40 parts of fresh dendrobe, 10-20 parts of kudzu root, 10-20 parts of coix seed, 2-5 parts of saffron crocus, 5-10 parts of ginseng and 5-10 parts of liquorice; taking fresh dendrobium officinale, smashing, mixing with other components of the fresh dendrobium antioxidant composition, decocting in water, extracting, taking filtrate, and concentrating to obtain antioxidant which can be used for preparing cosmetics. The invention has the beneficial effects that: the antioxidant extracted from the fresh dendrobium composition has high capability of removing OH free radicals.

Description

Fresh dendrobium antioxidant composition, preparation method and application of antioxidant

Technical Field

The invention belongs to the field of biological preparations, and particularly relates to a fresh dendrobe antioxidant composition, an antioxidant preparation method and application.

Background

As people age, various organs and cells of the body gradually age, the skin serving as a first barrier of the human body is the first thing, and particularly, the skin care of beauty-conscious women is important. Indeed, aging is a cellular oxidation process and thus it is necessary to combat the entire process, antioxidant being any substance that is present at low concentrations to effectively inhibit the oxidation of free radicals, either by acting directly on the free radicals or by indirectly consuming substances that readily generate free radicals, to prevent further reactions from occurring. The human body inevitably generates free radicals and also naturally generates antioxidant substances resisting the free radicals so as to counteract the oxidative attack of the free radicals on human cells. Research proves that the antioxidant system of a human body is a system which is comparable to the immune system and has perfect and complex functions, the stronger the antioxidant capacity of the body is, the healthier the body is, and when the antioxidant system of the body has problems, a formula capable of resisting oxidation is required to solve the problems.

The Chinese herbal medicine as the treasure of China is from nature, and the above problems can be solved by taking the theory of traditional Chinese medicine as guidance. Dendrobium officinale (Dendrobium huoshanense) is a famous and precious Chinese traditional medicine, and has the effects of nourishing yin, clearing heat, benefiting stomach, promoting fluid production, moistening lung, relieving cough and the like according to records of compendium of materia medica. Can greatly improve the level of SOD (main substance for delaying senility) in human bodies, and is very suitable for people who often stay up all night, excessively use brain, smoke and wine, and have weak and hypodynamia. The dendrobium officinale has the effects of improving eyesight, harmonizing yin and yang, tonifying yang and kidney, and beautifying and maintaining beauty, so that the effects of health care and life prolonging are achieved. The dendrobium officinale has strict requirements on the growth environment, is attached to cliff and tall trees, has more blossoms and few fruits, has extremely low seed germination rate under natural conditions, grows slowly and has higher requirements on temperature and humidity. In recent years, health care products and cosmetics developed by taking dendrobium officinale as a raw material are increasingly beautiful.

Disclosure of Invention

In order to solve the technical problems, the invention provides a fresh dendrobium antioxidant composition, an antioxidant preparation method and application.

The technical scheme adopted by the invention is as follows: a fresh herba Dendrobii antioxidant composition comprises 20-40 parts of fresh herba Dendrobii, 10-20 parts of radix Puerariae, 10-20 parts of Coicis semen, 2-5 parts of stigma croci Sativi, 5-10 parts of Ginseng radix, and 5-10 parts of Glycyrrhrizae radix.

A method for preparing antioxidant comprises collecting fresh herba Dendrobii, crushing, mixing with other components of fresh herba Dendrobii antioxidant composition, decocting in water, collecting filtrate, and concentrating to obtain antioxidant.

Preferably, the water extraction is carried out twice, the ratio of the first water extraction to the liquid is 1:20-25, the extraction time is 2-3h, the ratio of the second water extraction to the liquid is 1:10-15, and the extraction time is 0.5-1 h.

Preferably, the filtrate is concentrated to 1kg crude drug/L concentrated solution, and is stored in lyophilized powder form after freeze drying.

Antioxidant preparation method can be used for preparing antioxidant for cosmetic.

The invention has the advantages and positive effects that: the antioxidant extracted from the fresh dendrobium composition has high capability of removing OH free radicals, has good antioxidation effect when being used in cosmetics, does not stimulate the skin, and has warm-keeping function.

Drawings

FIG. 1 shows the DPPH.radical scavenging ratio of each component;

FIG. 2 shows the component pairs, OH radical scavenging rate.

Detailed Description

The invention relates to a fresh dendrobium antioxidant composition, and a preparation method and application of an antioxidant. The fresh dendrobium antioxidant composition comprises 20-40 parts of fresh dendrobium, 10-20 parts of kudzu root, 10-20 parts of coix seed, 2-5 parts of saffron, 5-10 parts of ginseng and 5-10 parts of liquorice.

Dendrobe: is a rare Chinese medicinal material in China, and has the effects of nourishing yin, clearing heat, benefiting stomach, promoting fluid production, moistening lung, relieving cough and the like according to records of compendium of materia medica. Can greatly improve the level of SOD (main substance for delaying senility) in human bodies, and is very suitable for people who often stay up all night, excessively use brain, smoke and wine, and have weak and hypodynamia. The dendrobium officinale has the effects of improving eyesight, harmonizing yin and yang, tonifying yang and kidney, and beautifying and maintaining beauty, so that the effects of health care and life prolonging are achieved. In recent years, numerous scholars have studied the effective components and effects of dendrobium officinale, and the dendrobium officinale mainly contains polysaccharides, amino acids, alkaloids and various inorganic elements and has the effects of resisting aging, resisting tumors, resisting oxidation, reducing blood sugar and the like. The polysaccharide is the main effective component of Dendrobium officinale, has effects of scavenging free radicals, resisting oxidation, regulating immunity, preventing and treating sub-health, reducing disease, inhibiting tumor, and delaying aging.

Fresh dendrobium: the dendrobium is a traditional fresh medicine, and the traditional Chinese medicine considers that the dendrobium has the characteristic of 'good living person', and gives an explanation on 'the fresh product'. Modern researches also show that fresh dendrobium has more remarkable pharmacological activity than dry dendrobium, so that 2020 edition Chinese pharmacopoeia introduces dry dendrobium and fresh dendrobium separately. At present, researches on chemical components and pharmacological activity of dendrobium mostly focus on dried dendrobium, and systematic and deep researches on differences of fresh dendrobium and dried dendrobium in chemical components and efficacy activity are lacked, so that market application and product development of fresh dendrobium are limited. The fresh dendrobium officinale strips can be directly used, so that the original effective components can be kept to the maximum extent; the dried product needs to be processed to a certain extent, which can affect the integrity of the original effective components of the dendrobium officinale more or less.

Kudzu root: the kudzu root contains starch as main component, flavone compound in 12%, amino acids and trace elements essential for human body. As for the health care value of breast enlargement and beauty, the isoflavone in the kudzuvine root is a phytoestrogen which is most similar to the estrogen secreted by the human body on the molecular structure at present, so the isoflavone can be combined with estrogen receptors on the surfaces of various systems and organ tissue cells of the body to play the effect. Compared with other medicines for regulating female endocrine, the pueraria isoflavone has the unique characteristics of preemptive combination with an estrogen receptor in vivo and mild action. Most of the common health-care cosmetic products such as the kudzu root powder and the like on the market are processed and are mixed with other components, so that the health-care cosmetic products can be properly used on the premise of ensuring the quality.

Saffron: according to the record of Ben Cao gang mu, Xie can activate blood, dominate heart qi and melancholy, and treat palpitation. Saffron has the functions of dredging meridian passage, eliminating blood stasis, eliminating stagnation, eliminating swelling, relieving pain, cooling blood, etc. Especially for people who are often in a 'stagnated state', if the people keep taking the medicine for a long time, the emotion of the people can be smooth, and the immunity of the human body can be comprehensively improved. Because the saffron can treat diseases such as hemophthisis, asthenia, irregular menstruation and the like, the saffron is beneficial to conditioning in the female body, the interior is healthy, and the face is naturally full of red light, so the saffron is always a hot door for health care and skin care of the female.

Ginseng: contains more than 100 effective components, mainly including ginsenoside, small amount of volatile oil, various amino acids and ginseng polypeptide, glucose, fructose, pectin, vitamins B1, B2, nicotinic acid, pantothenic acid, etc. Wherein ginsenoside is the most active substance, and has antiaging and antiinfectious effects. The ginseng exudate has no any adverse stimulation to the skin, can effectively resist cholesterol, help metabolism, promote skin blood circulation, enhance skin nutrition, and prevent skin dehydration, sclerosis and wrinkling, thereby keeping moisture in skin stratum corneum and enabling the skin to be tender, smooth and elastic. Ginseng also contains enzyme (tyrosinase) which can strongly inhibit melanin pigmentation, and can whiten and remove macula.

Coix seed: the five-cereal diet is the best staple food for losing weight, has the highest cellulose, low fat and low calorie in five cereals, has a miraculous effect on people with lower half body edema. More importantly, the pearl barley extract is rich in protein, can help to eliminate spots, has obvious curative effect on acne on the face and rough skin, can prevent alopecia and even has face slimming effect, can achieve sun protection and ultraviolet protection effect by adding the pearl barley extract into cosmetics, and has the functions of naturally whitening skin, improving skin metabolism and moisturizing. It can be used for enhancing moisture retention barrier of skin and preventing pigmentation.

Licorice root: has effects in invigorating spleen, invigorating qi, clearing away heat and toxic materials, expelling phlegm, relieving cough, and relieving spasm and pain. The liquorice has the most cooperation spirit, is one of the most widely applied medicines in the traditional Chinese medicine, can coordinate the medicines, has mild medicine property and silently exerts the effect of the liquorice. At present, a plurality of face cleaning and skin care products contain liquorice, and the liquorice is adopted as the soul component in a common herbal formula, so that the whitening effect is remarkable, the face cleaning and skin care products can be used for soothing damaged and sensitive skin, and meanwhile, the self-repairing capability of the skin can be improved, and the skin presents the texture of healthy skin from inside to outside and touches the skin.

The scheme adopts the fresh dendrobium, the kudzu-vine root, the coix seed, the saffron, the ginseng and the liquorice to be combined into the formula. Dendrobe is sweet and has the effects of tonifying stomach, promoting the production of body fluid, nourishing yin and clearing heat, and has the effects of nourishing yin, reducing fever and harmonizing yin and yang as a monarch drug in the formula. Radix puerariae is sweet, pungent and cool, has the effects of relieving muscles and fever, promoting the production of body fluid to quench thirst, promoting eruption, invigorating yang to arrest diarrhea, clearing and activating the channels and collaterals, is a Chinese and ministerial medicine in the formula, and has the effects of promoting qi circulation and invigorating yang to assist the dendrobium to nourish yin without stagnation. Ginseng is sweet, slightly bitter and warm. The traditional Chinese medicine composition has the effects of greatly tonifying primordial qi, recovering pulse, relieving depletion, tonifying spleen and lung, promoting the production of body fluid and nourishing blood, and soothing nerves and improving intelligence, is an adjuvant drug in the formula, and has the emphasis on tonifying qi and yang and promoting qi circulation without dispersing. Coix seed, semen coicis, sweet and bland in taste, cool in nature, promote diuresis and eliminate dampness, invigorate spleen and check diarrhea, remove arthralgia, expel pus, detoxify and dissipate stagnation, and is an adjuvant drug in the formula for eliminating dampness and expelling toxin. Saffron, sweet in flavor, neutral in nature, has the effects of promoting blood circulation to remove blood stasis, cooling blood and removing toxicity, and relieving depression and tranquilizing mind. Is an adjuvant drug in the formula and regulates qi and blood of the organism. The liquorice is sweet and neutral, and has the effects of tonifying spleen and qi, clearing away heat and toxic materials and harmonizing the drugs in the recipe. The medicines have the effects of regulating qi, blood, yin and yang and improving the functions of the human body; the components cooperate with each other, and when being used for cosmetics, the cosmetic has a better antioxidation effect and an outstanding nourishing function.

The active ingredients of the composition are extracted to obtain the antioxidant, and in order to ensure the antioxidant capacity of the antioxidant, fresh dendrobium officinale is selected in the scheme. The preparation method of antioxidant comprises taking fresh herba Dendrobii, crushing, and taking out; putting the treated herba Dendrobii into an extraction device, simultaneously adding other Chinese medicinal decoction pieces at the same time, and decocting with distilled water for 2 times, wherein the ratio of the first material to the second material is 1:20-25, the extraction time is 2-3h, the ratio of the second material to the second material is 1:10-15, and the extraction time is 0.5-1 h; filtering the extractive solution (100 mesh), and mixing filtrates; vacuum concentrating the filtrate at below 60 deg.C, and concentrating to obtain concentrated solution containing 1kg crude drug/L to obtain antioxidant; can be stored in lyophilized powder form after freeze drying. The obtained antioxidant has good free radical scavenging ability, and can be used for preparing cosmetics.

The present invention will be described with reference to specific examples, wherein the experimental methods without specific descriptions of the operation steps are performed according to the corresponding commercial specifications, and the instruments, reagents, and consumables used in the examples can be purchased from commercial companies without specific descriptions.

Example 1: preparation of antioxidants

The formula is as follows: 20-40 parts of fresh dendrobium, 10-20 parts of kudzu root, 10-20 parts of coix seed, 2-5 parts of saffron, 5-10 parts of ginseng and 5-10 parts of liquorice.

Selecting fresh dendrobium officinale, smashing and taking out for later use. Putting the treated herba Dendrobii into an extraction device, simultaneously adding other Chinese medicinal decoction pieces, and decocting with distilled water for 2 times, wherein the ratio of the material to the liquid at the 1 st time is 1:20, the extraction time is 2h, the ratio of the material to the liquid at the 2 nd time is 1:15, and the extraction time is 1 h. The extract was filtered (100 mesh) and the filtrates combined. The filtrate was concentrated under reduced pressure in vacuo at a temperature below 60 ℃. Concentrating to obtain concentrated solution containing 1kg crude drug/L, and freeze drying to obtain lyophilized powder.

Comparative example 1:

selecting fresh dendrobium officinale, smashing and taking out for later use. Putting the treated herba Dendrobii into an extraction device, adding distilled water, decocting with water for 2h at a ratio of 1:20 for the 1 st time, and 1:15 for the 2 nd time for 1h, and extracting for 2 times. The extract was filtered (100 mesh) and the filtrates combined. The filtrate was concentrated under reduced pressure in vacuo at a temperature below 60 ℃. Concentrating to obtain 1kg crude drug/L concentrated solution as fresh herba Dendrobii extract, and freeze drying to obtain lyophilized powder.

Comparative example 2:

selecting dry dendrobium officinale in the same producing area, and preparing the dry dendrobium officinale extract according to the preparation method in the comparative example 1.

Example 2: DPPH free radical scavenging experiment

0.1g of raw material freeze-dried powder is precisely weighed for example 1, comparative example 1 and comparative example 2 respectively, and dissolved by adding methanol in a dark environment to prepare a DPPH methanol solution with the concentration of 0.1 mmol/L. Firstly, precisely measuring 1mL of methanol and 1mL of double distilled water, fully and uniformly mixing, placing a mixed solution in a cuvette, taking the mixed solution as a blank solution at a wavelength of 517nm on an ultraviolet spectrophotometer, and carrying out zero setting; precisely measuring 1.0mL of 0.1mmol/LDPPH methanol solution, precisely measuring 1.0mL of sample solutions with different concentrations, adding the sample solutions, immediately and fully mixing, reacting for 30min in a dark place At room temperature, measuring and recording the absorbance value under the same condition, and marking as At; meanwhile, an Ac tube is arranged, 1.0mL of 0.1mmol/LDPPH methanol solution and 1.0mL of double distilled water are precisely measured and mixed, the mixture is fully and uniformly mixed, the positive control medicament L-ascorbic acid is treated by the same method, and the absorbance value is measured and recorded as Ac. Each concentration solution of each sample was subjected to 3 sets of parallel tests, and the calculated mean clearance was used as the final test result, which is shown in fig. 1 and table 1.

TABLE 1 component vs DPPH & free radical scavenging ratio

Concentration (mg/ml)	Dried dendrobe (%)	Fresh dendrobium (%)	Composition (%)	L-ascorbic acid (%)
					0.5	26.36	37.68	47.76	77.76
1	29.45	40.22	53.54	80.93
					1.5	35.78	45.16	61.24	86.35
2	38.89	47.85	64.79	88.57
					2.5	42.37	52.36	68.89	91.63

As can be seen from fig. 1 and the data in table 1, the composition has a stronger DPPH radical scavenging ability than the dry dendrobium extract and the fresh dendrobium extract alone, and the scavenging effect is gradually enhanced as the sample concentration is increased within a certain range. The clearance rate of the composition sample is superior to that of the dendrobium used alone, and the clearance rate range is 47.76-68.89%; the experimental result shows that the antioxidant extract of the composition has certain advantages in the aspect of the capability of removing DPPH free radicals.

Example 3: OH free radical scavenging experiments

OH free radical is a free radical with high activity and strong toxicity generated by organisms and has great damage effect on biomacromolecules, cells and the like, and the removal capability of the OH free radical is one of the common important indexes for evaluating and measuring the strength of the antioxidant capability of antioxidant substances.

Precisely measuring 1.0mL7.5mmol/L FeSO4, 1.0mL7.5mmol/L phenanthroline, 2.0mLPH7.4 phosphate buffer solution and 1.0mL8.8mmol/L H2O2 for example 1, comparative example 1 and comparative example 2 respectively, immediately mixing, fully mixing, adding 1.0mL of each sample solution with different concentrations and 4.0mL of double distilled water, mixing uniformly, and reacting at 37 ℃ for 30 min; a control sample of L-ascorbic acid was prepared in the same manner, and the absorbance was measured and recorded using an ultraviolet spectrophotometer at 536 nm.

TABLE 2 component pairs OH radical scavenging ratio

Concentration (mg/ml)	Dried dendrobe (%)	Fresh dendrobium (%)	Composition (%)	L-ascorbic acid (%)
					1	5.65	8.23	16.45	21.36
2	6.93	11.21	21.93	28.88
					3	7.54	14.73	27.55	36.53
4	8.55	17.47	32.87	42.95
					5	9.76	21.34	38.65	48.64

As shown in table 2 and fig. 2, the removing ability of each sample is gradually enhanced with the increase of the concentration, and the removing ability of the composition is better than that of the extracts of the dry dendrobium and the fresh dendrobium. Within the same concentration range, the removal rate of OH radicals from the composition samples ranged from 16.45 to 38.56%. Thus, the results of this test show that the compositions have certain advantages in their ability to scavenge the OH radicals.

As shown in the data of example 2 and example 3, the antioxidant capacity of the composition is still lower than that of L-ascorbic acid with antioxidant function, but the composition is a traditional Chinese medicine extract, the preparation process is simpler and is not chemically synthesized; when the extract is used in cosmetics, allergy is not easy to occur, and the traditional Chinese medicine extract is more compatible especially for users with sensitive skin.

The embodiments of the present invention have been described in detail, but the description is only for the preferred embodiments of the present invention and should not be construed as limiting the scope of the present invention. All equivalent changes and modifications made within the scope of the present invention shall fall within the scope of the present invention.

Claims

1. A fresh dendrobium antioxidant composition is characterized in that: comprises 20-40 parts of fresh dendrobium, 10-20 parts of kudzu root, 10-20 parts of coix seed, 2-5 parts of saffron, 5-10 parts of ginseng and 5-10 parts of liquorice.

2. A preparation method of an antioxidant is characterized by comprising the following steps: taking fresh dendrobium officinale, smashing, mixing with other components of the fresh dendrobium antioxidant composition in claim 1, decocting and extracting with water, taking filtrate, and concentrating to obtain antioxidant.

3. The method for producing an antioxidant according to claim 2, wherein: extracting with water twice at a ratio of 1:20-25 for 2-3 hr for the first time and at a ratio of 1:10-15 for 0.5-1 hr for the second time.

4. The method for producing an antioxidant according to claim 2 or 3, wherein: concentrating the filtrate to 1kg crude drug/L concentrated solution, freeze drying, and storing in lyophilized powder form.

5. Use of the antioxidant prepared by the process for the preparation of an antioxidant according to any one of claims 2 to 4 in cosmetics.