CN107019208A - A kind of Chinese medicine ferment and preparation method thereof - Google Patents
A kind of Chinese medicine ferment and preparation method thereof Download PDFInfo
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- CN107019208A CN107019208A CN201610728345.8A CN201610728345A CN107019208A CN 107019208 A CN107019208 A CN 107019208A CN 201610728345 A CN201610728345 A CN 201610728345A CN 107019208 A CN107019208 A CN 107019208A
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Abstract
The present invention relates to a kind of Chinese medicine ferment, it is made up of the component including following parts by weight：100~300 parts of Chinese medicine extract, 10~30 parts of bacterium solution, 10~20 parts of carbon source auxiliary material, 50~100 parts of water；The Chinese medicine extract is prepared from the following raw materials in parts by weight：30~50 parts of mulberry leaf, 30~60 parts of fig, 10~20 parts of American Ginseng, 50~80 parts of wilsonii, 20~50 parts of Gymnadenia conopsea, 10~15 parts of gynostemma pentaphylla, 10~15 parts of agate card, 10~15 parts of ganoderma lucidum, 10~15 parts of the red sage root, 15~25 parts of the Radix Astragali.The invention further relates to the preparation method of this Chinese medicine ferment.Stay in grade of the present invention, remains the activity and function of the active ingredient of Chinese medicine extract to greatest extent, can improve microcirculation, neutralizes free radical unnecessary in human body, anti-oxidant with antifatigue, anti-aging, effect of physical fitness.
Description
Technical field
The present invention relates to field of health care food, and in particular to a kind of Chinese medicine ferment and preparation method thereof.
Background technology
Ferment is, for raw material, to be fermented through multiple beneficial bacterium with one or more fresh vegetables, fruit, mushroom, Chinese herbal medicine etc.
Obtained from functional microbial fermented product.Ferment not only saves nutriment intrinsic in fermentation raw material, and production
Raw new bioactive ingredients and biology enzyme, with very strong ultra-oxygen anion free radical, hydroxy radical, DPPH radicals scavengings
Ability, can discharge soda acid in toxin, balanced body, and then repair cell and adjustment constitution.Ferment can be generally divided into acidifying also
Former ferment, transferase, hydrolyzable ferment, disengaging ferment, opposite sexization ferment and the major class of NOS six.
With the development and the raising of living standards of the people of life science, natural medicinal plant product is gradually by people's
Favor, the demand to natural medicinal plant gradually develops to efficient, hypotoxicity direction, therefore, it is necessary to research and develop more with day
Right medicinal plant meets the market demand for the novel microbial ferment product of raw material.
The content of the invention
The first object of the present invention is to provide a kind of Chinese medicine ferment with excellent health-care efficacy.
It is a further object of the present invention to provide the preparation method of the Chinese medicine ferment.
In order to reach the purpose of the present invention, present invention employs following technical scheme：
A kind of Chinese medicine ferment, it is characterised in that：It is made up of the component including following parts by weight：
The Chinese medicine extract is prepared from the following raw materials in parts by weight：
It is preferred that, the Chinese medicine ferment can also be made up of the component including following parts by weight：
It is preferred that, the Chinese medicine extract can also be prepared from the following raw materials in parts by weight：
Contain 1~100 parts by weight water and 1~10 parts by weight strain in the bacterium solution.
Lu Shi Zygosaccharomyceses containing 1~20 parts by weight in the strain, the monascus of 1~25 parts by weight and 1~
The lactobacillus acidophilus of 30 parts by weight.
The carbon source auxiliary material is by 30~50 parts by weight lactose, 20~60 parts by weight honey and 10~50 parts by weight sea
Mosanom is constituted.
A kind of preparation method of Chinese medicine ferment, it is characterised in that including the step of it is as follows：
A. Chinese medicine extract
(1) mulberry leaf, fig, American Ginseng, wilsonii, Gymnadenia conopsea, gynostemma pentaphylla, agate card, spirit are weighed by formulation weight number
Sesame, the red sage root and the Radix Astragali, are crushed, with water according to 1:5~200 weight is than mixing, stirring immersion, ultrasonic extraction, and the time is 10~
40min, ultrasonic power is 200~500W, and temperature is 50~80 DEG C, and boiling is heated to the mixture after extraction, and filtering is obtained
First filtrate and filter residue；
(2) by filter residue and water according to 1:5~200 weight is than mixing, and ultrasonic extraction, the time is 10~40min, and power is
200~500W, temperature is 50~80 DEG C, and boiling is heated to the mixture after extraction, and filtering obtains the second filtrate and filter residue；
(3) the first filtrate and the second filtrate are merged, centrifuges, obtain Chinese medicine extract；
B. ferment for the first time
The honey and sodium alginate in carbon source auxiliary material are weighed by formulation weight number, adds in Chinese medicine extract and mixes, so
Afterwards by formula consumption access Lu Shi Zygosaccharomyceses and monascus, in 25~35 DEG C of temperature, 250~300L/min of oxygen-supplying amount, infiltration
Fermented 5~10 days under conditions of 0.07~0.08Mpa of pressure, pH value 3~4, obtain first time zymotic fluid；
C. second of fermentation
Lactose and lactobacillus acidophilus are added by formula consumption in first time zymotic fluid, in 30~40 DEG C of temperature, oxygen-supplying amount
Fermented 5~10 days under conditions of 0.5~1.5L/min, 0.07~0.08Mpa of osmotic pressure, pH value 2~4, obtain second of fermentation
Liquid；
D. ferment maturation
Temperature is constant at 38~40 DEG C, continues to ferment 100~180 days, obtains total liquid that ferments.
To finally ferment total liquid ultrasonic cryogenic concentration, add 1~20 parts by weight vinegar and glucose is seasoned, filter,
Obtain enzyme stoste；Or as needed can be with low temperature drying into powdered.
It is worth noting that, the parts by weight that the strain and water of the present invention are related to are set when being using bacterium solution as an entirety
, the parts by weight that Lu Shi Zygosaccharomyceses, monascus and lactobacillus acidophilus of the invention is related to are to be used as an entirety using strain
When set, those skilled in the art can also add other components on the basis of formula of the present invention.
The component and the source of strain that the present invention is used are commercially available, the experimental method of unreceipted actual conditions of the invention,
Generally carried out according to normal condition, or condition recommended by the manufacturer is carried out.
Compared with prior art, the beneficial effects of the invention are as follows：Stay in grade, remains Chinese medical extract to greatest extent
The activity and function of active ingredient, can improve microcirculation, free radical unnecessary in human body be neutralized, with antifatigue, antioxygen
Change, anti-aging, effect of physical fitness.
Specific embodiment
In order to preferably illustrate present invention, illustrated below with some preferably specific embodiments.But these have
Body embodiment is simply to illustrate that present disclosure, rather than present invention is limited.
Principle explanation
Mulberry leaf, sweet-bitter flavor is cold in nature, not only containing decortication sterone, middle knee sterone, lupenone, mulberry glycosides and micro rue
Glycosides, but also contain folic acid, fumaric acid, copper, zinc and phytoestrogen etc.；With help, anti-aging, stable vegetative nerve,
Reduce and eliminate and produce the effects such as stagnating lipofuscin (senile plaque expelling) in human body skin or internal organ, and the human body longevity can be extended
Life.
Fig is a kind of high nutrition, high medicinal novel dietotherapy health fruit, suffered from diarrhoea with stomach invigorating, gut purge, treatment,
The effects such as hemorrhoid, improving eyesight are promoted the production of body fluid is controlled in subdhing swelling and detoxicating, treatment pharynx, and its fruit contains protein 1.0%, carbohydrate 12.6%, crude fibre
1.9%, in drying fruit sugar content be up in 75% or so, fresh fruit sugar amount in 15~20%, pulp containing a large amount of vitamin As,
Vitamin D, organic acid, trace element and amino acid.
American Ginseng (Panax quinquefolius) is Araliaceae Panax herbaceos perennial, alias American ginseng, production
In America ＆ Canada, panaxoside, a small amount of volatile oil, carbohydrate and amino acid, with regulation central nervous system are planted containing more than ten
System, antifatigue, enhancing organism adaptation ability, anti-arrhythmia cordis, resist myocardial ischemia, strengthen myocardial contractive power, promotion adrenal gland skin
The effects such as matter hormone secretion, enhancing immunity of organism.
Wilsonii is studies on plants of Acanthopanax Miq. in Araliaceae, also known as slender acanthopanax ginseng, tiger nail, pricker etc., with regulation endocrine, anti-
Fatigue, anti-aging, anti-aging, antitumor, the effects such as improving sleep quality.
Gymnadenia conopsea, also known as palm ginseng, negative and positive ginseng, are orchid family herbaceos perennials, originate in the ground such as Tibet, Qinghai, growth is slow
Slowly seem very precious, be described as " magical not old grass " and " desert ginseng ".It has benefiting qi and nourishing blood, kidney tonifying moistening lung, life
Tianjin is quenched the thirst, and in effect for calming the nerves with body-building and tonifying yang, can effectively alleviate neurasthenia；Brain circlulation, brain tonic can be increased to promote
Memory, it is antifatigue promotion gonad function, improve immunity, help anti-ageing anti-cancer.
Gynostemma pentaphylla also known as black seven leaves courage, are cucurbitaceae genus gynostemma, have significant inhibitory action to a variety of cancer cells,
Not only can with reducing blood lipid, blood pressure, blood glucose, and can with antifatigue, antiulcer, while there is calmness, it is hypnosis, unobstructed stool, flat
Breathe heavily cough-relieving, the hemorrhoid that disappear, fat-reducing, treatment asthenic symptoms, the formation of suppression gall stone and preventing and treating glucocorticoid side effect and extension cell longevity
The effects such as life.
Agate card has enhancing energy, improves fertility, improves sexual function, treatment climacteric metancholia, rheumatism, depression
And the effects such as anemia.
Ganoderma lucidum has antitumor and immunological regulation, hypoglycemic, liver protection, anti-aging, anti-inflammatory and antalgic, cardioprotection and anti-freezing
Blood is acted on
The red sage root is promoting blood circulation and removing blood stasis, regulating qi-flowing for relieving pain Chinese patent drug, and there is cardiac stimulant to strengthen myocardial contractive power, improve heart work(for it
Can, increase myocardial blood flow, expand peripheral vascular, blood flow increase, antithrombus formation improves plasmin activity；Extend, blood coagulation
Time, suppress platelet aggregation, improve microcirculation, the reparation of promotion organization and palingenesis：The reparation and regeneration of promotion organization,
Liver protection improves liver microcirculation and has inhibitory action to external staphylococcus, Escherichia coli, denaturation bacillus.
The Radix Astragali be it is most basic in Chinese medicine be used to improve lung and the medicinal material of respiratory system immunocompetence, with tonifying Qi and enhancement
The effects such as spleen function and promotion metabolic capability；Also there is reduction blood viscosity, reduce thrombosis, reduce blood pressure, protect
Heart, bidirectional modulation blood glucose, Green Tea Extract damage, anti-aging, anti anoxia, antitumor, enhancing immunity of organisms effect, can be used to
Treat the diseases such as heart disease, hypertension, diabetes.The Radix Astragali can also expand blood vessel, improve skin blood circulation and nutrition condition, therefore right
The long disunion person of chronic ulcer is effective.It can also eliminate the albuminuria of nephritis patient, protect liver, prevent hepatic glycogen from reducing.
Above-mentioned component is under the excellent compatibility that the present invention is recorded, and drug effect obtains significant Synergistic, can be in maximum limit
Degree remains the activity of active ingredient, is effectively improved microcirculation and removes the unnecessary free radical of human body, antifatigue, anti-aging,
Physical fitness.
Six kinds of traditional Chinese medicinal components of the above press mulberry leaf：Fig：American Ginseng：Wilsonii：Gymnadenia conopsea：Gynostemma pentaphylla=4:5:1:6:3:
Synergistic after 1 combination, compared to addition mulberry leaf, fig, American Ginseng, wilsonii, Gymnadenia conopsea and gynostemma pentaphylla in ferment merely
Effect it is more preferable.
Embodiment 1
A kind of Chinese medicine ferment, is made up of the component including following parts by weight：
The Chinese medicine extract is prepared from the following raw materials in parts by weight：
Contain 50 parts by weight water and 10 parts by weight strains in the bacterium solution.
Lu Shi Zygosaccharomyceses containing 20 parts by weight, the monascus of 25 parts by weight and 30 parts by weight in the strain
Lactobacillus acidophilus.
The carbon source auxiliary material is made up of 30 parts by weight lactose, 20 parts by weight honey and 50 parts by weight sodium alginates.
The preparation method of the Chinese medicine ferment, including the step of it is as follows：
A. Chinese medicine extract
(1) mulberry leaf, fig, American Ginseng, wilsonii, Gymnadenia conopsea, gynostemma pentaphylla, agate card, spirit are weighed by formulation weight number
Sesame, the red sage root and the Radix Astragali, are crushed to 50 mesh, are then well mixed respectively, with water according to 1:100 weight is soaked than mixing, stirring,
Ultrasonic extraction, the time is 10min, and ultrasonic power is 500W, and temperature is 60 DEG C, and boiling is heated to the mixture after extraction,
Filtering, obtains the first filtrate and filter residue；
(2) by filter residue and water according to 1:100 weight is than mixing, ultrasonic extraction, and the time is 10min, and power is 500W, temperature
Spend for 60 DEG C, boiling is heated to the mixture after extraction, filtering obtains the second filtrate and filter residue；
(3) the first filtrate and the second filtrate are merged, centrifuges, obtain Chinese medicine extract；
B. ferment for the first time
The honey and sodium alginate in carbon source auxiliary material are weighed by formulation weight number, adds in Chinese medicine extract and mixes, so
It is 25 DEG C, oxygen-supplying amount 250L/min, osmotic pressure in temperature afterwards by formula consumption access Lu Shi Zygosaccharomyceses and monascus bacterium solution
Fermented 8 days under conditions of 0.08Mpa, pH value 3~4, obtain first time zymotic fluid；
C. second of fermentation
Lactose and lactobacillus acidophilus are added by formula consumption in first time zymotic fluid, in 30 DEG C of temperature, oxygen-supplying amount 0.5L/
Fermented 6 days under conditions of min, osmotic pressure 0.07Mpa, pH value 2~4, obtain second of zymotic fluid；
D. ferment maturation
The temperature of second of zymotic fluid is constant at 40 DEG C, and fermentation maturation 100 days obtains total liquid that ferments, finally will fermentation
Total liquid ultrasonic cryogenic concentration, adds 15 parts by weight vinegar and glucose is seasoned, filter, obtain enzyme stoste.
Embodiment 2
A kind of Chinese medicine ferment, is made up of the component including following parts by weight：
The Chinese medicine extract is prepared from the following raw materials in parts by weight：
Contain 60 parts by weight water and 8 parts by weight strains in the bacterium solution.
Lu Shi Zygosaccharomyceses containing 10 parts by weight, the monascus of 20 parts by weight and 20 parts by weight in the strain
Lactobacillus acidophilus.
The carbon source auxiliary material is made up of 50 parts by weight lactose, 60 parts by weight honey and 10 parts by weight sodium alginates.
The preparation method of the Chinese medicine ferment, including the step of it is as follows：
A. Chinese medicine extract
(1) mulberry leaf, fig, American Ginseng, wilsonii, Gymnadenia conopsea, gynostemma pentaphylla, agate card, spirit are weighed by formulation weight number
Sesame, the red sage root and the Radix Astragali, are crushed to 90 mesh, are then well mixed respectively, with water according to 1:200 weight is soaked than mixing, stirring,
Ultrasonic extraction, the time is 40min, and power is 200W, and temperature is 50 DEG C, and boiling is heated to the mixture after extraction, and filtering is obtained
First filtrate and filter residue；
(2) by filter residue and water according to 1:200 weight is than mixing, ultrasonic extraction, and the time is 40min, and power is 200W, temperature
Spend for 50 DEG C, boiling is heated to the mixture after extraction, filtering obtains the second filtrate and filter residue；
(3) the first filtrate and the second filtrate are merged, centrifuges, obtain Chinese medicine extract；
B. ferment for the first time
The honey and sodium alginate in carbon source auxiliary material are weighed by formulation weight number, adds in Chinese medicine extract and mixes, so
Afterwards by formula consumption access Lu Shi Zygosaccharomyceses bacterium solution and monascus bacterium solution, in 35 DEG C of temperature, oxygen-supplying amount 300L/min, osmotic pressure
Fermented 8 days under conditions of 0.07Mpa, pH value 3~4, obtain first time zymotic fluid；
C. second of fermentation
Lactose and lactobacillus acidophilus bacterium solution are added by formula consumption in first time zymotic fluid, in 40 DEG C of temperature, oxygen-supplying amount
Fermented 10 days under conditions of 1.5L/min, osmotic pressure 0.08Mpa, pH value 2~4, obtain second of zymotic fluid；
D. ferment maturation
Second of zymotic fluid is constant at 40 DEG C in temperature, ferment 120 days, obtain total liquid that ferments, will finally ferment total liquid
Ultrasonic cryogenic is concentrated, and adds 20 parts by weight vinegar and glucose is seasoned, filter, obtain enzyme stoste.
Embodiment 3
A kind of Chinese medicine ferment, is made up of the component including following parts by weight：
The Chinese medicine extract can also be prepared from the following raw materials in parts by weight：
The Chinese medicine extract, bacterium solution, carbon source auxiliary material, the ratio of weight and number of water are 10：1：1：4.
Contain 30 parts by weight water and 5 parts by weight strains in the bacterium solution.
Lu Shi Zygosaccharomyceses containing 15 parts by weight, the monascus of 10 parts by weight and 10 parts by weight in the strain
Lactobacillus acidophilus.
The carbon source auxiliary material is made up of 40 parts by weight lactose, 30 parts by weight honey and 30 parts by weight sodium alginates.
The preparation method of the Chinese medicine ferment, including the step of it is as follows：
A. Chinese medicine extract
(1) mulberry leaf, fig, American Ginseng, wilsonii, Gymnadenia conopsea, gynostemma pentaphylla, agate card, spirit are weighed by formulation weight number
Sesame, the red sage root and the Radix Astragali, are crushed to 70 mesh, are then well mixed respectively, with water according to 1:150 weight is soaked than mixing, stirring,
Ultrasonic extraction, the time is 30min, and power is 300W, and temperature is 60 DEG C, and boiling is heated to the mixture after extraction, and filtering is obtained
First filtrate and filter residue；
(2) by filter residue and water according to 1:150 weight is than mixing, ultrasonic extraction, and the time is 30min, and power is 300W, temperature
Spend for 60 DEG C, boiling is heated to the mixture after extraction, filtering obtains the second filtrate and filter residue；
(3) the first filtrate and the second filtrate are merged, centrifuges, obtain Chinese medicine extract；
B. ferment for the first time
The honey and sodium alginate in carbon source auxiliary material are weighed by formulation weight number, adds in Chinese medicine extract and mixes, so
Afterwards by formula consumption access Lu Shi Zygosaccharomyceses and monascus, in 30 DEG C of temperature, oxygen-supplying amount 300L/min, osmotic pressure
Fermented 5 days under conditions of 0.075Mpa, pH value 3~4, obtain first time zymotic fluid；
C. second of fermentation
Lactose and lactobacillus acidophilus bacterium solution are added by formula consumption in first time zymotic fluid, in 35 DEG C of temperature, oxygen-supplying amount
Fermented 5 days under conditions of 1L/min, osmotic pressure 0.075Mpa, pH value 2~4, obtain second of zymotic fluid；
D. ferment maturation
Second of zymotic fluid is mixed in temperature constant at 38 DEG C, fermentation maturation 180 days obtains total liquid that ferments, finally will hair
The total liquid ultrasonic cryogenic concentration of ferment, adds 20 parts by weight vinegar and glucose is seasoned, filter, obtain enzyme stoste.
Detect data
The index for the enzyme stoste that the present invention is obtained is as follows：
Fragrance pure taste,
Sugar≤6 g/l
10~15 g/l of total acid
PH (25 DEG C) 2.0~4.0
Protein (g/100mL) >=2.5
Soluble solid (g/100mL) >=2.5
Lead (in terms of Pb) (mg/L)≤0.2
Copper (in terms of Cu) (mg/L)≤5.0
Total plate count (cuf/ml)≤100
Escherichia coli≤3
Mould≤10
Salmonella must not detect
Shigella must not detect
Staphylococcus aureus must not detect.
Experiment proves one
In order to verify the security of ferment prepared by the present invention, animal acute toxicity test is carried out：
Sample：Enzyme stoste prepared by embodiment 1-3.
Experimental animal：Small white mouse, weight average is in 40g or so, male and female random selection.
Method and result：Take small white mouse 40, be divided into 4 groups, every group 10, wherein the 1st, 2,3 groups be used as test group, the 4th
Group is as a control group, hungry 15 hours or so, is mixed into the zymogen of embodiment 1-3 preparations by every each 20ml in feed
Liquid, three times a day, continuously feeds 5 days, surveys body weight.
As a result show that small white mouse activity is normal, non-toxic reaction, no death, i.e., without acute toxicity, and test group is with compareing
Group compares, and the small white mouse of test group is full of vitality, increased weight, i.e. embodiment 1-3 prepare enzyme stoste have it is antifatigue and
Effect of physical fitness.
Experiment proves one
In order to verify the security of ferment prepared by the present invention, long-term toxicity test for animals has been carried out：
Sample：Enzyme stoste prepared by embodiment 1-3.
Experimental animal：Big white mouse, weight average is in 130g or so, male and female random selection.
Method and result：Take big white mouse 40, be divided into 4 groups, every group 10, wherein the 1st, 2,3 groups be used as test group, the 4th
Group diet is as a control group, hungry 15 hours or so, and embodiment 1-3 preparations are mixed into by every each 20ml in feed
Enzyme stoste, once a day, continuously feeds 90 days, surveys body weight.
As a result show that big white mouse activity is normal, non-toxic reaction, no death, i.e., without long property toxicity, and test group is with compareing
Group compares, and the big white mouse of test group is full of vitality, increased weight, i.e. embodiment 1-3 prepare enzyme stoste have it is antifatigue and
Effect of physical fitness.
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (5)
1. a kind of Chinese medicine ferment, it is characterised in that：It is made up of the component including following parts by weight：
The Chinese medicine extract is prepared from the following raw materials in parts by weight：
2. a kind of Chinese medicine ferment according to claim 1, it is characterised in that：Contain 1~100 parts by weight in the bacterium solution
Water and 1~10 parts by weight strain.
3. a kind of Chinese medicine ferment according to claim 2, it is characterised in that：Contain 1~20 parts by weight in the strain
Lu Shi Zygosaccharomyceses, the lactobacillus acidophilus of the monascus of 1~25 parts by weight and 1~30 parts by weight.
4. a kind of Chinese medicine ferment according to claim 3, it is characterised in that：The carbon source auxiliary material is by 30~50 parts by weight
Lactose, 20~60 parts by weight honey and 10~50 parts by weight sodium alginates composition.
5. a kind of preparation method of Chinese medicine ferment according to claim 3, it is characterised in that including the step of it is as follows：
A. Chinese medicine extract
(1) mulberry leaf, fig, American Ginseng, wilsonii, Gymnadenia conopsea, gynostemma pentaphylla, agate card, ganoderma lucidum, pellet are weighed by formulation weight number
Ginseng and the Radix Astragali, are crushed, with water according to 1:5~200 weight is soaked than mixing, stirring, ultrasonic extraction, and the time is 10~40min,
Ultrasonic power is 200~500W, and temperature is 50~80 DEG C, and boiling is heated to the mixture after extraction, and filtering obtains the first filter
Liquid and filter residue；
(2) by filter residue and water according to 1:5~200 weight is than mixing, and ultrasonic extraction, the time is 10~40min, and power is 200
~500W, temperature is 50~80 DEG C, and boiling is heated to the mixture after extraction, and filtering obtains the second filtrate and filter residue；
(3) the first filtrate and the second filtrate are merged, centrifuges, obtain Chinese medicine extract；
B. ferment for the first time
The honey and sodium alginate in carbon source auxiliary material are weighed by formulation weight number, adds in Chinese medicine extract and mixes, then press
Consumption access Lu Shi Zygosaccharomyceses and monascus are formulated, in 25~35 DEG C of temperature, 250~300L/min of oxygen-supplying amount, osmotic pressure
Fermented 5~10 days under conditions of 0.07~0.08Mpa, pH value 3~4, obtain first time zymotic fluid；
C. second of fermentation
Lactose and lactobacillus acidophilus are added by formula consumption in first time zymotic fluid, in 30~40 DEG C of temperature, oxygen-supplying amount 0.5~
Fermented 5~10 days under conditions of 1.5L/min, 0.07~0.08Mpa of osmotic pressure, pH value 2~4, obtain second of zymotic fluid；
D. ferment maturation
Temperature is constant at 38~40 DEG C, continues to ferment 100~180 days, obtains total liquid that ferments.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107549790A (en) * | 2017-09-06 | 2018-01-09 | 戴琪 | A kind of preparation method for ferment of losing weight |
| CN111631401A (en) * | 2020-06-09 | 2020-09-08 | 承德医学院 | Traditional Chinese medicine enzyme and preparation method thereof |
| CN111671855A (en) * | 2020-06-23 | 2020-09-18 | 西藏大学 | Preparation method and application of genkwa-ginseng Tibetan medicine composition |
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| CN105249062A (en) * | 2015-09-08 | 2016-01-20 | 青海华牛生物科技有限公司 | Plateau plant enzyme drink and preparation method therefor |
| CN105595349A (en) * | 2015-09-08 | 2016-05-25 | 青海华牛生物科技有限公司 | Plateau plant solid ferment food |
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| CN102670917A (en) * | 2012-01-20 | 2012-09-19 | 华子昂 | Composite enzyme and its preparation method |
| CN103844266A (en) * | 2014-03-25 | 2014-06-11 | 容瑜 | Natural conditioning and nourishing ferment and manufacturing method thereof |
| CN105249062A (en) * | 2015-09-08 | 2016-01-20 | 青海华牛生物科技有限公司 | Plateau plant enzyme drink and preparation method therefor |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107549790A (en) * | 2017-09-06 | 2018-01-09 | 戴琪 | A kind of preparation method for ferment of losing weight |
| CN111631401A (en) * | 2020-06-09 | 2020-09-08 | 承德医学院 | Traditional Chinese medicine enzyme and preparation method thereof |
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Application publication date: 20170808 |