CN106860814B

CN106860814B - Health food composition for improving female frequent micturition and urinary incontinence and preparation method and application thereof

Info

Publication number: CN106860814B
Application number: CN201710062636.2A
Authority: CN
Inventors: 郎伟君
Original assignee: Harbin Quick Good Pharmaceutical Co Ltd
Current assignee: Letai Pharmaceutical Co.,Ltd.
Priority date: 2017-01-22
Filing date: 2017-01-22
Publication date: 2020-02-18
Anticipated expiration: 2037-01-22
Also published as: CN106860814A

Abstract

The invention discloses a health-care food composition for improving female frequent micturition and urinary incontinence, a preparation method and application thereof, and belongs to the technical field of health-care foods. The composition of the invention mainly comprises the following raw material components in parts by weight: 0.4-5 parts of pumpkin seed oil, 0.4-4 parts of pumpkin seed water extract, 15-30 parts of Chinese yam, 0.6-3 parts of sharpleaf galangal fruit water extract, 0.4-5 parts of pumpkin seed oil, 0.4-5 parts of pumpkin seed water extract, 0.5-3 parts of Chinese angelica water extract and 0.5-2 parts of oyster. The invention adopts a unique extraction method, has high utilization rate of effective components, small waste, good effect and no toxic or side effect.

Description

Health food composition for improving female frequent micturition and urinary incontinence and preparation method and application thereof

Technical Field

The invention relates to a health-care food composition, in particular to a health-care food composition for improving female frequent micturition and urinary incontinence, a preparation method and application thereof, belonging to the technical field of health-care food.

Background

The aging of the population is one of the major challenges facing the international society at present, and all countries bring the aging of the population into the basic national policy. At present, the elderly in the United states aged 65 and older account for about 12.5% of the national population, and will reach 20.7% in 2050, with the population aged 85 and older reaching 1800 and more than ten thousand, which is nearly 6 times as large as that in 1995. In 2005-2030, the aged population of the European Union over 65 years will increase by 52.3%. According to the national institute of statistics of economic and social development bulletin 2015, 22200 million people in 60 years old and above account for 16.1 percent of the total population, 14386 million people in 65 years old and above account for 10.5 percent of the total population (21242 million people in 60 years old and above, 15.5 percent of the total population, 13755 million people in 65 years old and above account for 10.1 percent of the total population in 2014). The population number of the old people is increased by 4.7 percent, and the patients suffering from the urinary system will continuously rise along with the aging of the population and the increase of the average life of the people. The clinical manifestations of the disease are frequent micturition, urgency, nocturia, incontinence and the like, and the western medicine considers that women are mainly caused by overactive bladder. The diseases not only cause physiological pain and psychological troubles for patients, but also bring much trouble to children and even influence the normal life of the whole family.

The production and excretion of urine plays an extremely critical role in maintaining the water metabolism balance of the body. The urinary bladder is the organ of the human body that stores and discharges urine, but both the production and excretion of urine must depend on the action of kidney qi. The function of steaming kidney qi is normal, the function of promoting and regulating kidney yin and kidney yang is coordinated, and urine can be normally produced and excreted only when the bladder is properly opened and closed. When the kidney is gasified normally, the bladder is opened and closed normally, turbid liquid is discharged, and if the kidney is gasified frequently, the opening and closing of the bladder are affected, so that the opening and closing of the bladder are not favorable, and symptoms such as oliguria, edema and the like are seen; or the bladder is not restricted, and polyuria and enuresis are caused. The abundance or insufficiency of kidney qi directly affects the qi transformation and opening and closing of the urinary bladder. Resulting in the bladder being open or closed unfavorably or the bladder being restricted unwarranbly, and with symptoms of polyuria, enuresis, etc.

Urinary incontinence is the involuntary outflow of urine without conscious control of the micturition function, is a common symptom of dysuria disorders and essentially represents a dysfunction of the bladder in storing urine, and is not an independent disease but a result of some disorders affecting the function of the bladder and urethra. 30% of the elderly suffer from varying degrees of urinary incontinence and have significant gender differences, with women having a higher prevalence than men. The prevalence rate of stress urinary incontinence of the elderly women is 29.0 percent, which is obviously higher than the prevalence rate of the elderly men 11.9 percent. Along with the aging of society, frequent micturition and urinary incontinence become social problems, particularly, frequent micturition and urinary incontinence limit the action range of patients, the life quality of the patients is obviously reduced, and western medicines have poor treatment effect, high recurrence rate and great side effect and bring heavy burden to the patients.

The function of human organs is gradually reduced along with the increase of age, which causes various discomfort and inconvenience, and the frequent nocturia of the old is one of the outstanding cases. The traditional Chinese medicine considers that the kidney is the congenital foundation, mainly holds water and opens into two yin. The senile patients with kidney yang deficiency and decline of the fire from the gate of life belonging to yin at night, which worsens the deficiency of yang qi and fails to control the bladder, resulting in increased nocturia.

The invention researches the pathogenesis of female frequent micturition and urinary incontinence on the basis of treatment based on syndrome differentiation, carefully selects raw materials, and adopts a unique squeezing and extracting method to prepare the health-care food for improving the female frequent micturition and the urinary incontinence. The health food composition comprises 8 kinds of pumpkin seed oil, pumpkin seed water extract, Chinese yam, sharpleaf galangal fruit water extract, pumpkin seed oil, pumpkin seed water extract, Chinese angelica water extract and oyster. The cucurbita pepo seeds in the prescription have the effects of clearing heat and promoting urination, relieving restlessness and quenching thirst, moistening lung and arresting cough, and reducing swelling and dissipating stagnation, and can be used for treating edema and abdominal distension, polydipsia, sore toxicity, nephritis, ascites due to cirrhosis and other symptoms. Pumpkin seeds are used as main components, and other 7-flavor components such as pumpkin seeds and the like are used as auxiliary components; the pumpkin seeds are sweet in taste and warm in nature, enter spleen and stomach channels, tonify middle-jiao and qi, diminish inflammation and relieve pain; the yam is sweet in flavor, mild and nontoxic. Meridian tropism entered spleen, lung and kidney meridians; has the effects of warming spleen, stopping diarrhea, controlling saliva, warming kidney, reducing urination and stopping emission. Sharpleaf galangal fruit is commonly used for deficiency-cold of spleen and stomach, vomiting, diarrhea, cold pain in abdomen, excessive saliva in mouth, enuresis due to kidney deficiency, frequent micturition, spermatorrhea and whitish and turbid urine. Mu Li is indicated for frequent urination and stranguria . The medicines are combined to play the effects of tonifying qi and kidney, dredging collaterals and contracting spring and improving the female frequent micturition and urinary incontinence. In addition, the invention also adopts a cold pressing method to process the raw materials, and the pressed cake is further processed by adopting a water extraction mode to obtain effective components, so that the raw materials are fully utilized; fructus Alpinae Oxyphyllae, radix Angelicae sinensis, and Concha Ostreae can be added during extraction process, and has effects of warming spleen, relieving diarrhea, controlling saliva, warming kidney, reducing urination, and stopping nocturnal emission. The angelica contains abundant zinc element, and the oyster contains various amino acids, vitamins and other substances. The invention firstly tries to combine pumpkin seed oil and water extract and simultaneously add Chinese angelica, oyster and other substances to enhance the curative effect, has no toxic or side effect, ensures higher utilization rate of effective components, fully utilizes the performance of raw materials, and has small waste and good effect. The inventor finally determines the following technical scheme of the invention through repeated experiments, summarization and improvement, and obtains more ideal treatment effect.

One possible technical solution is: a method for preparing health food for improving female frequent micturition and urinary incontinence is provided. The method is characterized in that: the raw materials comprise 0.4-5 parts of pumpkin seed oil, 15-30 parts of Chinese yam and 0.6-3 parts of fructus alpiniae oxyphyllae water extract. The treatment method comprises the following steps: the yam is crushed after steam sterilization, pumpkin seeds and pumpkin seeds are directly squeezed to obtain pumpkin seed oil, and the fructus alpiniae oxyphyllae active substances are extracted by a water extraction method.

The disadvantages are as follows: 1. the direct squeezing method has the advantages of simple process operation, simple and complicated production equipment, flexible production scale and suitability for the production of various vegetable oils, but the squeezing method has the defects of low oil yield, large labor force and low production efficiency. 2. The single component is applied or 1-2 raw materials are combined, and the variety is single.

Disclosure of Invention

The invention aims to provide a health-care food composition capable of improving female frequent micturition and urinary incontinence and a preparation method and application thereof.

In order to achieve the purpose, the invention adopts the following technical means:

the invention provides a health-care food composition for improving female frequent micturition and urinary incontinence, which is prepared from the following raw material medicines in parts by weight: 0.4-5 parts of pumpkin seed oil, 0.4-4 parts of pumpkin seed water extract, 15-30 parts of Chinese yam, 0.6-3 parts of sharpleaf galangal fruit water extract, 0.4-5 parts of pumpkin seed oil, 0.4-5 parts of pumpkin seed water extract, 0.5-3 parts of Chinese angelica water extract and 0.5-2 parts of oyster; preferably, the traditional Chinese medicine is prepared from the following raw material medicines in parts by weight: 2.5 parts of pumpkin seeds, 2.5 parts of pumpkin seed water extract, 15 parts of Chinese yam, 1.5 parts of sharpleaf galangal fruit water extract, 2.5 parts of pumpkin seed oil, 2.5 parts of pumpkin seed water extract, 1.5 parts of Chinese angelica water extract and 1 part of oyster.

Further, the present invention provides a preparation method for the above health food composition, comprising the steps of:

(1) sterilization

Sterilizing rhizoma Dioscoreae, drying, pulverizing, and sieving to obtain material A;

sterilizing oysters, drying, crushing and sieving to obtain a material B for later use;

(2) cold pressing for extraction

Peeling pumpkin seeds, taking the seeds, and squeezing the pumpkin seeds to obtain pumpkin seed oil, namely the material C and a squeezed pumpkin seed cake for later use;

husking pumpkin seeds, taking out seeds, and squeezing the pumpkin seeds to obtain pumpkin seed oil, namely the material D and a squeezed pumpkin seed cake for later use;

(3) water extraction

Extracting the pressed pumpkin seed cake, the angelica and the fructus alpiniae oxyphyllae by a water extraction method respectively, filtering, concentrating and drying to obtain water extracts of pumpkin seeds, angelica and fructus alpiniae oxyphyllae which are respectively materials E-H for later use;

(4) mixing

Weighing eight materials A-H according to the formula, mixing uniformly, and granulating to obtain the final product.

Wherein, preferably, the sterilization treatment in the step (1) is steam sterilization treatment; and the sieving is to sieve the mixture by a sieve of 80-100 meshes.

Preferably, the pumpkin seed kernel oil pressing in the step (2) is cold pressing by using an oil press, the temperature of a chamber of the oil press is adjusted to be 70 ℃, the pressure is adjusted to be 3.5Mpa, the rotating speed is adjusted to be 36r/min, and the process is repeated for 3-4 times.

Preferably, the oil pressing of the pumpkin seeds in the step (2) is cold pressing by using an oil press, the temperature of a chamber of the oil press is adjusted to be 80 ℃, the pressure is adjusted to be 3.6Mpa, and the rotating speed is adjusted to be 36r/min, and the operation is repeated for 3-4 times.

Wherein, preferably, the weight ratio of the feed liquid extracted by the water in the step (3) is 1: 10-1: 20.

the health-care food composition can be used for preparing health-care food for improving female frequent micturition and urinary incontinence.

The functional effects of the components in the formula of the invention are as follows:

pumpkin seeds: pumpkin (cucurbita moschata Duch.) is also called guava, cucurbita sativa, etc., and is an annual herb plant of the cucurbitaceae family. The pumpkin has high nutritive value, contains various functional components besides various nutrient components such as free amino acids, carotene, VB, VC, mannitol and the like, and has more researches on pumpkin at home and relatively late research on pumpkin seeds. The pumpkin seeds are mature seeds of pumpkins, and contain rich unsaturated fatty acid, vitamins, bioflavonoids, pumpkin polysaccharide, mineral substances and the like, and the functional components have wide and important biological functions and have good research and application values. The pumpkin seed oil is oil extracted from fully mature pumpkin seeds, is natural and dark green, is fresh and non-greasy, has a nutlet taste, is rich in nutrient components, can provide required nutrient substances for a human body, and is one of high-content nutrient oils. The pumpkin seed oil is obtained by squeezing or extracting pumpkin seeds, and contains oleic acid, linoleic acid, linolenic acid, palmitic acid, stearic acid, arachidic acid, vaccenic acid, erucic acid and other unsaturated fatty acids, wherein the total unsaturated fatty acids account for 74.9% of the total fatty acids, and the content of the oleic acid and the linoleic acid accounts for 35% -50% of the dry weight of the pumpkin seeds. Unsaturated fatty acids contain polyunsaturated fatty acids (PUFA) as the main component; the pumpkin seed oil also contains abundant vitamins such as fat-soluble VE and a certain amount of phylloquinone-vitamin K; the pumpkin seed oil is rich in mineral substances, wherein zinc, magnesium, iron, selenium and the like have special biological effects. Particularly, phytosterol contained therein can treat urinary system disease symptoms of BPH patients, and in conventional medicine, the seeds thereof are used as anthelmintic, emollient, diuretic and tonic, and a tea made from the seeds thereof can treat prostatic hypertrophy. Therefore, the pumpkin seeds have good development prospect in the field of health-care food.

Pumpkin seeds: cucurbita pepo L, also known as Cucurbita aurantia willd, Cucurbita melopepo L. The eating of zucchini has been described by ancient people since the time of the first century, and it has been known since then in China and other countries. The medical scientists in the past consistently acknowledged that it has six functions: nourishing and caring skin, nourishing heart and tranquilizing mind, invigorating stomach and moistening lung, resisting bacteria and strengthening body constitution, loosening bowel to relieve constipation, and relieving chest stuffiness. In addition, it can be used for adjuvant treatment of edema, abdominal distention, polydipsia, skin sore, nephritis, and ascites due to liver cirrhosis. In the conventional medicine, the seeds thereof are used as anthelmintic, emollient, diuretic and tonic, and a tea prepared from the seeds thereof can treat prostatic hypertrophy. There are currently examples of the use of zucchini seeds for the treatment of urological disorders associated with Benign Prostatic Hypertrophy (BPH). The principal ingredients of the cucurbita pepo seeds are phytosterols, and the active compounds are delta 5, delta 7 and delta 8-sterols, wherein the delta 7-sterol is a key active ingredient with high content in the cucurbita pepo seeds and is considered to be used for treating benign prostatic hyperplasia, a small amount of delta 5 and delta 8-sterols also exist in the cucurbita pepo seeds and play a role, and high content of carotenoid, polyunsaturated fatty acid and fat-soluble vitamin also have certain active effects, so that the cucurbita pepo seeds can treat urinary system disease symptoms of BPH patients, and have good development prospects in the field of health foods. At the end of the 19 th century, pumpkin seed oil was used for the treatment of urinary tract disorders. Animal studies have shown that water extracts of cucurbita pepo seeds may improve bladder and urethral function.

Chinese yam: is dried rhizome of Dioscorea opposita (DfcKSCorea opposita Thunb.) belonging to Dioscoreaceae. Digging after withering of the stem and leaf in winter, cutting off the root head, cleaning, removing the outer skin and fibrous root, and drying, which is called Maoshan tablet; or removing outer skin, cutting into thick pieces, and drying to obtain SHANYAO tablet; optionally selecting dry rhizoma Dioscoreae, soaking in water until there is no dry center, sealing, cutting, rubbing with wood plate into cylinder, sun drying, and polishing, and is called "Dioscorea Opposita". Nature and taste: sweet and neutral. Meridian tropism: it enters spleen, lung and kidney meridians. The efficacy is as follows: tonify spleen and stomach, promote the production of body fluid and nourish lung, tonify kidney and astringe essence. Can be used for treating spleen deficiency, anorexia, chronic diarrhea, lung deficiency, cough, asthma, spermatorrhea, leukorrhagia, frequent micturition, and diabetes due to deficiency heat. The bran-parched rhizoma Dioscoreae has effects of invigorating spleen and invigorating stomach. Can be used for treating spleen deficiency, anorexia, diarrhea, loose stool, and leukorrhagia.

Fructus alpiniae oxyphyllae: fructus Alpinae Oxyphyllae is fruit of Alpinia oxyphylla Miq. Collected in summer and autumn when the fruit turns red from green, dried in the sun or at low temperature. The seeds are in irregular oblate shape, slightly blunt edges, the diameter is about 3mm, the surface is gray brown or gray yellow, and the seeds are externally coated with light brown membranous aril; hard, white endosperm. Has special fragrance, pungent and slightly bitter taste. Nature and taste: pungent and warm in flavor. Meridian tropism: it enters spleen and kidney meridians. The efficacy is as follows: warm kidney, secure essence and reduce urination, warm spleen, check diarrhea and check saliva. Can be used for treating renal deficiency, enuresis, frequent micturition, nocturnal emission, diarrhea due to spleen cold, abdominal psychroalgia, and excessive saliva. Modern pharmacological research shows that fructus alpiniae oxyphyllae contains volatile oil, fructus alpiniae oxyphyllae ketone, vitamin B1, B2, C, E, various amino acids and the like.

Chinese angelica: angelica sinensis is one of the most commonly used Chinese herbs, and is derived from dried roots of Angelica sinensis (Oliv.) Diels, a plant of Umbelliferae. It is warm in nature, sweet and pungent in flavor. It enters liver, heart and spleen meridians; has effects in replenishing blood, promoting blood circulation, regulating menstruation, relieving pain, and loosening bowel to relieve constipation; can be used for treating blood deficiency, sallow complexion, giddiness, palpitation, menoxenia, amenorrhea, dysmenorrhea, asthenia cold, abdominal pain, rheumatalgia, traumatic injury, superficial infection, pyocutaneous disease, intestinal dryness, and constipation. Except the prescription of the traditional Chinese medicine, the Chinese patent medicine containing angelica can reach more than 80. Meanwhile, angelica is also a raw material for health food prescribed by the ministry of health of china, and is often eaten as a tonic in daily life.

Oyster shell: the Concha Ostreae is the shell of Ostrealgas Thun-berg, Ostreasuris Crassostrea (Ostreasuraria), or Ostrealileenhanensis Crosse (Ostrealileenhanensis), which is distributed along the sea in China, and can be harvested all the year round, unprocessed or calcined, and smashed when used. It is salty, astringent and slightly cold in flavor, enters liver and kidney meridians, and has the actions of pacifying liver and subduing yang, softening and resolving hard mass, astringing and arresting discharge, and all the medical books of the past generation have detailed records on the functions and indications of oysters. Mu Li is first listed in Shen nong Ben Cao Jing (Shen nong's herbal), which is listed as the top grade in the book. Wherein the oyster is recorded with salty and flat taste, and mainly treats typhoid fever, chills and fever, warm malaria and spilt, fright anger anger, and can be used for treating spasm and rat fistula, leukorrhagia of women with red and white color, strengthening bone joints, killing pathogenic qi and prolonging the life. Until the Han Dynasty, Shang Zhong Jing written in Shang Han Lun and jin Kui Yao L ü e, oyster was widely used to form prescriptions for treating diseases. The number of prescriptions related to the compatibility of oyster in the two books is more than 11. The works show the important position of the oysters in the clinical application of the traditional Chinese medicine to different degrees. Recent studies show that: the oyster contains 18 amino acids, glycogen, B vitamins, taurine, calcium, phosphorus, iron, zinc and other nutrients, and can improve the immunity of the organism after being eaten frequently, and the taurine contained in the oyster can reduce the blood fat and the blood pressure. Oyster is a clinical commonly used Chinese medicine in the record of Chinese pharmacopoeia of the calendar edition.

The raw material medicaments used by the invention all meet the quality standard requirements of health-care food.

Compared with the prior art, the invention has the beneficial effects that:

the cucurbita pepo seeds in the prescription have the effects of clearing heat and promoting urination, relieving restlessness and quenching thirst, moistening lung and arresting cough, and reducing swelling and dissipating stagnation, and can be used for treating edema and abdominal distension, polydipsia, sore toxicity, nephritis, ascites due to cirrhosis and other symptoms. Pumpkin seeds are used as main components, and other 7-flavor components such as pumpkin seeds and the like are used as auxiliary components; the pumpkin seeds are sweet in taste and warm in nature, enter spleen and stomach channels, tonify middle-jiao and qi, diminish inflammation and relieve pain; the yam is sweet in flavor, mild and nontoxic. Meridian tropism entered spleen, lung and kidney meridians; has the effects of warming spleen, stopping diarrhea, controlling saliva, warming kidney, reducing urination and stopping emission. Sharpleaf galangal fruit is commonly used for deficiency-cold of spleen and stomach, vomiting, diarrhea, cold pain in abdomen, excessive saliva in mouth, enuresis due to kidney deficiency, frequent micturition, spermatorrhea and whitish and turbid urine. Mu Li is indicated for frequent urination and stranguria . The medicines are combined to play the effects of tonifying qi and kidney, dredging collaterals and contracting spring and improving the female frequent micturition and urinary incontinence. In addition, the invention also adopts a cold pressing method to process the raw materials, and the pressed cake is further processed by adopting a water extraction mode to obtain effective components, so that the raw materials are fully utilized; fructus Alpinae Oxyphyllae, radix Angelicae sinensis, and Concha Ostreae can be added during extraction process, and has effects of warming spleen, relieving diarrhea, controlling saliva, warming kidney, reducing urination, and stopping nocturnal emission. The angelica contains abundant zinc element, and the oyster contains various amino acids, vitamins and other substances. The invention firstly tries to combine pumpkin seed oil and water extract and simultaneously add Chinese angelica, oyster and other substances to enhance the curative effect, has no toxic or side effect, ensures higher utilization rate of effective components, fully utilizes the performance of raw materials, and has small waste and good effect. And obtains more ideal treatment effect.

Detailed description of the invention

The invention will be further described with reference to specific comparative examples and examples, and the advantages and features of the invention will become more apparent as the description proceeds. These examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.

Comparative example 1

(1) Cold pressing for extraction

Peeling semen Cucurbitae, collecting seed kernel, cold pressing semen Cucurbitae with oil press, adjusting the temperature of oil press chamber to 70 deg.C, pressure to 3.5Mpa, and rotation speed to 36r/min, repeating for 4 times to obtain semen Cucurbitae oil, material A, and pressed semen Cucurbitae cake;

(2) water extraction

Extracting the pressed pumpkin seed cake by adopting a water extraction method, wherein the extraction temperature is 100 ℃, the extraction time is 1 hour, and the weight ratio of the material liquid to the material liquid is 1:10, filtering, concentrating and drying to obtain the pumpkin seed water extract B for later use;

(3) mixing

Weighing 5g of the material A and 5g of the material B according to the formula ratio, uniformly mixing, and granulating to obtain the feed.

Comparative example 2

(1) Cold pressing for extraction

peeling Cucurbita pepo seeds, taking out seeds, cold pressing the Cucurbita pepo seeds by using an oil press, adjusting the temperature of a barrel of the oil press to 80 ℃, the pressure to 3.6Mpa and the rotating speed to 36r/min, and repeating for 4 times to obtain Cucurbita pepo seed oil, namely a material B and a pressed Cucurbita pepo seed cake for later use;

(2) water extraction

Extracting the pressed pumpkin seed cake and the pressed pumpkin seed cake by a water extraction method respectively at the extraction temperature of 100 ℃ for 1 hour, wherein the weight ratio of the material liquid to the liquid is 1:10, filtering, concentrating and drying to respectively obtain water extracts of pumpkin seeds and pumpkin seeds, namely C, D for later use;

(3) mixing

Weighing 2.5g of the material A, 2.5g of the material B, 2.5g of the material C and 2.5g of the material D according to the formula ratio, uniformly mixing, and granulating to obtain the pellet.

Comparative example 3

(1) Cold pressing for extraction

(2) water extraction

Extracting the pressed pumpkin seed cake, the pressed pumpkin seed cake and the fructus alpiniae oxyphyllae by a water extraction method respectively, wherein the extraction temperature is 100 ℃, the extraction time is 1 hour, and the weight ratio of material liquid is 1: 20, filtering, concentrating and drying to respectively obtain water extracts of pumpkin seeds, pumpkin seeds and sharpleaf galangal fruit, namely the materials C-E for later use;

(3) mixing

Weighing 2.5g of the material A, 2.5g of the material B, 2.5g of the material C, 2.5g of the material D and 1.5g of the material E according to the formula ratio, uniformly mixing, and granulating to obtain the composite material.

Comparative example 4

(1) Sterilization

Sterilizing rhizoma Dioscoreae with steam, drying, pulverizing, and sieving with 100 mesh sieve to obtain material A;

(2) cold pressing for extraction

Peeling semen Cucurbitae, collecting seed kernel, cold pressing semen Cucurbitae with oil press, adjusting the temperature of oil press chamber to 70 deg.C, pressure to 3.5Mpa, and rotation speed to 36r/min, repeating for 4 times to obtain semen Cucurbitae oil, material B, and pressed semen Cucurbitae cake;

peeling Cucurbita pepo seeds, taking out seeds, cold pressing the Cucurbita pepo seeds by using an oil press, adjusting the temperature of a barrel of the oil press to 80 ℃, the pressure to 3.6Mpa and the rotating speed to 36r/min, and repeating for 4 times to obtain Cucurbita pepo seed oil, namely a material C and a pressed Cucurbita pepo seed cake for later use;

(3) water extraction

Extracting the pressed pumpkin seed cakes, the pressed pumpkin seed cakes and the bitter cardamom respectively by a water extraction method at the extraction temperature of 100 ℃ for 1 hour and with the material liquid weight ratio of 1:10, filtering, concentrating and drying to respectively obtain water extracts of the pumpkin seeds, the pumpkin seeds and the bitter cardamom, namely the materials D-F for later use;

(4) mixing

Weighing 15g of the material A, 2.5g of the material B, 2.5g of the material C, 2.5g of the material D, 2.5g of the material E and 1.5g of the material F according to the formula ratio, uniformly mixing, and granulating to obtain the composite material.

Comparative example 5

(1) Sterilization

(2) cold pressing for extraction

(3) water extraction

Extracting the pressed pumpkin seed cakes, the angelica and the sharpleaf galangal fruit by a water extraction method respectively at the extraction temperature of 100 ℃ for 1 hour in a material liquid weight ratio of 1:10, filtering, concentrating and drying to obtain water extracts of pumpkin seeds, angelica and sharpleaf galangal fruit which are respectively materials D-G for later use;

(4) mixing

Weighing 15G of the material A, 2.5G of the material B, 2.5G of the material C, 2.5G of the material D, 2.5G of the material E, 1.5G of the material F and 1.5G of the material G according to the formula ratio, uniformly mixing, and granulating to obtain the composite material.

Example 1

(1) Sterilization

sterilizing oysters by steam, drying, crushing, and sieving by a 100-mesh sieve to obtain a material B for later use;

(2) cold pressing for extraction

Peeling semen Cucurbitae, collecting seed kernel, cold pressing semen Cucurbitae with oil press, adjusting the temperature of oil press chamber to 70 deg.C, pressure to 3.5Mpa, and rotation speed to 36r/min, repeating for 4 times to obtain semen Cucurbitae oil, material C, and pressed semen Cucurbitae cake;

peeling Cucurbita pepo seeds, taking out seeds, cold pressing the Cucurbita pepo seeds by using an oil press, adjusting the temperature of a barrel of the oil press to 80 ℃, the pressure to 3.6Mpa and the rotating speed to 36r/min, and repeating for 4 times to obtain Cucurbita pepo seed oil, namely a material D and a pressed Cucurbita pepo seed cake for later use;

(3) water extraction

Extracting the pressed pumpkin seed cakes, the angelica and the sharpleaf galangal fruit by a water extraction method respectively at the extraction temperature of 100 ℃ for 1 hour and the weight ratio of material liquid of 1:10, filtering, concentrating and drying to obtain water extracts of pumpkin seeds, angelica and sharpleaf galangal fruit which are respectively materials E-H for later use;

(4) mixing

Weighing 20G of the material A, 2G of the material B, 1.25G of the material C, 1.25G of the material D, 1.25G of the material E, 1.25G of the material F, 1G of the material G and 1G of the material H according to the formula ratio, uniformly mixing, and granulating to obtain the composite material.

Example 2

(1) Sterilization

(2) cold pressing for extraction

(3) water extraction

(4) mixing

Weighing 15G of material A, 0.5G of material B, 3.1G of material C, 3.1G of material D, 3.1G of material E, 3.1G of material F, 0.5G of material G and 0.6G of material H according to the formula ratio, uniformly mixing, and granulating to obtain the composite material.

Example 3

(1) Sterilization

(2) cold pressing for extraction

(3) water extraction

(4) mixing

Weighing 15G of the material A, 1G of the material B, 2.5G of the material C, 2.5G of the material D, 2.5G of the material E, 2.5G of the material F, 1.5G of the material G and 1.5G of the material H according to the formula ratio, uniformly mixing, and granulating to obtain the composite material.

Experimental example 1 pharmacodynamic test of the pharmaceutical composition of the present invention for improving female pollakiuria and urinary incontinence

1. Experimental Material

And (3) testing the sample: compositions prepared according to comparative examples 1-5 and examples 1-3. Control drug: atropine. Animal species: healthy female rabbits. Animal body weight: 1.3-1.5 kg.

2. Experimental methods

Before the experiment, the rabbits are fasted for 24 hours, and the drinking water is not limited. During the experiment, the bladder and the urethra are enabled to be stunned, and the bladder and the urethra are rapidly taken out; the bladder is cut off along the middle of the ventral aspect, the urethra is cut off by a spiral method, and the bladder is rinsed in Krebs solution for later use. The bladder was fixed in a culture dish containing Krebs solution, the mucosal layer was gently removed, and two longitudinal muscle strips were cut along the direction of the longitudinal muscle fibers at the two sides of the dorsal aspect of the bladder body with a fixed surgical blade. Suspending bladder muscle strips in a perfusion muscle trough filled with Krebs liquid, keeping the temperature of the solution at 37 ℃, continuously introducing mixed gas with the volume fraction of 95% oxygen and 5% carbon dioxide, inputting signals into a two-channel physiological recorder through a muscle tension transducer, recording isometric contraction activities of the muscle strips, replacing fresh Krebs liquid once every 20 minutes, and respectively adding the compositions of comparative examples 1-5 and examples 1-3 and atropine when the spontaneous activities of the muscle strips are stable after about 30 minutes, wherein the administration dose of the composition is 3g/kg, and the administration dose of the atropine is 0.1 mg/kg. Each group treated 8 rabbits and the results are shown in table 1.

TABLE 1 Effect of the compositions of comparative examples 1-5 and examples 1-3 and the control on the contractile activity of isolated bladder smooth muscle strips in rabbits

Note: and represents comparison P < 0.01 after and before drug addition.

3. Conclusion

As can be seen from Table 1, the compositions of comparative examples 1-5 and examples 1-3 all significantly reduced the frequency and amplitude of contraction of the bladder smooth muscle, and the effect thereof showed a more and more significant trend from comparative examples 1-5 to examples 1-3, with the effect of example 3 being most significant. The drug effect of the combination of pumpkin seeds and pumpkin seeds is higher than that of a single component, and the bitter cardamon, the Chinese yam, the Chinese angelica and the oyster shell all play a role in promoting the drug effect of the composition.

Experimental example 2 clinical study of the pharmaceutical composition of the present invention on female frequent micturition, urgency of urination, and urinary incontinence

Frequent micturition and urgent micturition are common diseases, the incidence rate of the diseases is gradually increased with the increase of the age, the pathogenesis is more complex, and an effective treatment method is lacked at present. The frequency and urgency of urination can reach 20-30 times/day without increasing the total amount of urine. The patient feels urgent micturition but does not feel pain, and the daily life of the patient is seriously affected.

1. Clinical data and methods

1.1 clinical data and grouping

60 female patients with frequent micturition and urgent micturition with the disease course of more than 1 month are selected from 2016 to 2016 and 9, and are randomly divided into 2 groups of 30 persons, namely a control group and an example 3 group.

1.2 inclusion criteria

Gender is female, age is 35-55 years, and night urination frequency is more than 3 on average.

1.3 diagnostic criteria

The traditional Chinese medicine symptoms comprise intolerance of cold, cold limbs, frequent micturition, qi deficiency, weakness, soreness of waist and knees, pale tongue with white fur, and patients with bladder and urethra organic lesions and frequent micturition and urgent micturition caused by tumor, renal failure and the like.

1.4 methods of treatment

The control group adopts conventional western medicine treatment, and takes racanisodamine tablet (5 mg/tablet, 2 tablets once, 3 times a day); the composition of example 3 was dosed to the group of example 3 (25 g/dose, 3 times a day).

1.5 Observation index

According to the usual work and rest of the patient, the patient can relieve the urine from getting up to sleep to the next morning, and the urine frequency, the urine volume and the continuous recording are carried out for 7 days. And (6) averaging. The observation is carried out for 1 time respectively before taking medicine, 3 months after taking medicine and after the treatment course is finished.

1.6 Effect assessment

The symptom is completely disappeared to be cured, the symptom is obviously relieved to be effective, the symptom which is relieved but not reach 50 percent is effective, and the symptom which is not relieved or even aggravated is ineffective.

2. Results

TABLE 2 index of frequent nocturia in control group

	Before treatment	The treatment period is 3 months	The treatment period is 6 months
				Number of times of urination	4.6±1.2	3.1±1.0^△	2.1±0.8*
Night urine volume (ml)	648.1±113.5	520.1±112.1^△	390±105.4*

Note:^△p < 0.05 compared to pre-treatment, P < 0.05 compared to 3 months of treatment.

TABLE 3 example 3 index changes of group of frequent nocturia

	Before treatment	The treatment period is 3 months	The treatment period is 6 months
				Number of times of urination	4.8±1.4	2.6±1.3^△	2.0±0.8*
Night urine volume (ml)	650.3±124.1	510.2±134.1^△	378.3±135.1*

TABLE 4 comparison of the therapeutic effects of the example 3 and control groups

	Number of examples	Show effect	Is effective	Total effective
					Control group	30	10(33.3％)	11(36.6％)	21(69.9％)
EXAMPLE 6 group	30	12(40％)*	13(43.3％)*	25(83.3％)*

Note: p < 0.05 compared to control.

The results show that the control group and the administration group have improved nocturia frequency after taking the medicine, and the average nocturia frequency and the nocturia volume are obviously reduced, which shows that the health-care food composition has certain improvement effect on female frequent micturition and urgent micturition.

Claims

1. A health food composition for improving female frequent micturition and urinary incontinence is characterized by comprising the following raw material components in parts by weight: 0.4-5 parts of pumpkin seed oil, 0.4-4 parts of pumpkin seed water extract, 15-30 parts of Chinese yam, 0.6-3 parts of sharpleaf galangal fruit water extract, 0.4-5 parts of pumpkin seed oil, 0.4-5 parts of pumpkin seed water extract, 0.5-3 parts of Chinese angelica water extract and 0.5-2 parts of oyster.

2. The health food composition according to claim 1, which is prepared from the following raw materials in parts by weight: 2.5 parts of pumpkin seeds, 2.5 parts of pumpkin seed water extract, 15 parts of Chinese yam, 1.5 parts of sharpleaf galangal fruit water extract, 2.5 parts of pumpkin seed oil, 2.5 parts of pumpkin seed water extract, 1.5 parts of Chinese angelica water extract and 1 part of oyster.

3. A process for preparing a health food composition according to claim 1 or 2, comprising the steps of:

(1) sterilization

(2) cold pressing for extraction

(3) water extraction

(4) mixing

Weighing eight materials A-H according to the parts by weight of the materials in the claim 1 or 2, uniformly mixing, and granulating to obtain the compound feed.

4. The production method according to claim 3, wherein the sterilization treatment in the step (1) is a steam sterilization treatment; and the sieving is to sieve the mixture by a sieve of 80-100 meshes.

5. The preparation method according to claim 3, wherein the squeezing of the pumpkin seeds in the step (2) is cold pressing by using an oil press, and the temperature of a chamber of the oil press is adjusted to 70 ℃, the pressure is adjusted to 3.5Mpa, and the rotation speed is adjusted to 36r/min, and the steps are repeated for 3-4 times.

6. The preparation method according to claim 3, wherein the oil pressing of the pumpkin seeds in the step (2) is cold pressing by using an oil press, and the temperature of a chamber of the oil press is adjusted to 80 ℃, the pressure is adjusted to 3.6MPa, and the rotation speed is adjusted to 36r/min, and the steps are repeated for 3-4 times.

7. The preparation method according to claim 3, wherein the water extraction in the step (3) is performed at an extraction temperature of 100 ℃, for an extraction time of 1 hour, and in a feed-liquid weight ratio of 1: 10-1: 20.

8. use of the health food composition of claim 1 or 2 for preparing a health food for improving female pollakiuria and urinary incontinence.