CN107549790A - A kind of preparation method for ferment of losing weight - Google Patents
A kind of preparation method for ferment of losing weight Download PDFInfo
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Abstract
The invention discloses a kind of preparation method for ferment of losing weight, belong to healthy food field.Present invention water intaking, peptone, sodium chloride, agar, are well mixed, and high-temperature sterilization makes solid medium；Saccharomycete inoculation medium inclined-plane is obtained into slant strains；Water intaking, peptone, sodium chloride, agar, it is well mixed, high-temperature sterilization makes liquid medium；Slant strains are gone in nutrient solution, culture is prepared into yeast fermentation broth.Ping Guo ﹑ Fan Qie ﹑ citrus You Guo ﹑ grapes, lemon is taken to add NaCl solution immersion, the raw material cleaning after immersion, then raw material is cut into slices drying, take drying object；Drying object is placed into immersion in Chinese herbal medicine fluid and filters to obtain filter residue；Lv Zha ﹑ NaCO3 Rong Ye ﹑ alginic acids are stirred, room temperature is cooled to after well mixed heating, obtain mixing filter residue, then add bacterium solution into mixing filter residue and carry out the zymotic fluid that ferments to obtain；Zymotic fluid is subjected to vacuum freeze drying, produces fat-reducing ferment.The beneficial effects of the invention are as follows：Degreasing effect is more preferable, and nutrition is higher.
Description
Technical field
The invention belongs to healthy food technical field, and in particular to a kind of preparation method for ferment of losing weight.
Background technology
Ferment is also known as enzyme, is the active macromolecules caused by organism with catalytic action.It is each in organism
Kind biochemical reactions, will nearly all be carried out under the catalytic action of ferment.Modern society's dog-eat-dog, rhythm of life are accelerated,
Environmental pollution, age increase, and can all cause the ferment in human body to reduce.When internal ferment declines, various diseases just occur
Shape.Some developed countries and the way to keep in good health of area edible ferment prevailing, not only to it is healthy, while sexy, seedling can be kept
The stature of bar.
Ferment mainly includes probiotics and enzyme.Probiotics includes saccharomycete, lactic acid bacteria, aspergillus etc..Small part probiotics
(Ru Yueshi lactobacillus) is fat-reducing probiotics, after the intake of Yue Shi lactobacillus Yue Shi lactobacillus, is colonized in enteron aisle.The effect of fat-reducing
Mechanism is：1st, sugar decomposition is citric acid, and coacetylase is converted into the presence of citrate lyase, directly results in sugar and is converted into fat
Fat accumulation is internal.Yue Shi lactobacillus reduces citrate lyase secretion, blocks accumulation fat in the formation and acceleration bodies of fat
Oxidative metabolism；2nd, liver and gall secretion cholate directly participates in the metabolism of fat, and highly-water-soluble cholate forms fat after being combined with cholesterol
Accumulation is internal.Yue Shi lactobacillus promotes the secretion of cholate hydrolytic enzyme, and cholate is lost water solubility turns into low aqueous solubility cholate, and
Precipitation is combined to form with cholesterol to excrete, and is blocked the formation of fat and is reduced the content of cholesterolemia.Main enzyme has egg
White enzyme, lipase, superoxide dismutase, amylase etc..Lipase also has the function that fat-reducing, and it is usually day that it, which hydrolyzes substrate,
Right grease, hydrolysis position are the ester bonds that aliphatic acid is connected with glycerine in grease.Losing weight the presence of probiotics and lipase can be with
Develop the ferment product with exclusive weight losing function.There are thousands of kinds of ferment products on domestic and international market at present, but single-minded have
The fat-reducing ferment product of effect is blank.And some product fat-reducing effects at present on the market are not fully up to expectations, so, production
A kind of good fat-reducing ferment of effect is extremely urgent.
The content of the invention
The technical problems to be solved by the invention：For currently for some product fat-reducing effects at present on the market not to the utmost
Such as people's will, or even there are certain harm and rebound effect problem to human body, there is provided a kind of preparation method for ferment of losing weight.
In order to solve the above technical problems, the present invention is using technical scheme as described below：
A kind of preparation method for ferment of losing weight, it is characterised in that the preparation method comprises the following steps：
（1）According to the mass fraction, following raw material is taken, 2 ~ 3 parts of 2 ~ 3 parts of Ping Guo ﹑, 4 ~ 5 parts of Fan Qie ﹑ citrus You Guo ﹑, 5 ~ 6 parts of Ti ﹑ 2 ~
3 portions of lemons are put into NaCl solution, soak 2 ~ 3h, the raw material after must soaking, then water are poured into the raw material after immersion, cleaning,
Raw material is cut into slices again, is dried at 40 ~ 50 DEG C, takes drying object；
（2）Drying object is placed 10 ~ 12h is soaked in Chinese herbal medicine fluid, filtering, obtain filter residue；
（3）In mass ratio 1：3~4：2 ~ 3, by Lv Zha ﹑ NaCO3Rong Ye ﹑ alginic acids are stirred, be well mixed after heating 20 ~
30min, room temperature is cooled to, adds the bacterium solution of filter residue quality 15 ~ 18%, fermented, obtain fermenting mixture, zymotic fluid is entered
Row vacuum freeze drying, freeze-drying thing is collected, produces fat-reducing ferment.
The step（2）Middle Chinese herbal medicine fluid is the Fen ﹑ Chinese yams of 2 ~ 3 Fen ﹑ dried orange peels of green tea 2 ~ 3 Fen ﹑ light-coloured vinegars, 1 ~ 2 Fen ﹑ radix pseudostellariaes 2 ~ 3
8 ~ 10 parts of water compositions of the Fen ﹑ iron mountain of 2 ~ 3 Fen ﹑ malt, 2 ~ 3 Fen ﹑ hawthorn 2 ~ 32 ~ 3 Fen ﹑ matrimony vines of thin ginseng, 3 ~ 4 Fen ﹑.
The step（3）Middle bacterium solution is yeast fermentation broth, the preparation method of yeast fermentation broth：
A. count in parts by weight, take 8 ~ 10 parts of water, 20 ~ 30 parts of peptones, 10 ~ 15 parts of sodium chloride, 4 ~ 10 parts of agar, mixing is
It is even, high-temperature sterilization, make solid medium；
B. by resistance to high sugar yeast bacterium inoculation medium inclined-plane, 2 ~ 4d is cultivated at 27 ~ 29 DEG C of temperature, obtains slant strains, it is standby；
C. count in parts by weight, take 70 ~ 80 parts of water, 20 ~ 30 parts of peptones, 10 ~ 15 parts of sodium chloride, 4 ~ 10 parts of agar, mixing is
It is even, high-temperature sterilization, make liquid medium；
D. slant strains standby in step b are directly forwarded in nutrient solution, at 27 ~ 29 DEG C of temperature, cultivate 2 ~ 4d, prepare
Obtain yeast fermentation broth.
The step（3）Middle vacuum drying is temperature -15 ~ -10 DEG C, 1.3 ~ 13Pa of vacuum.
Compared with other method, advantageous effects are the present invention：The raw material of the present invention is all high fine fruit, let us
Rate of metabolism accelerates, so as to lose weight.Chinese herbal medicine by unnecessary moisture content in vivo and can accumulate in internal metabolism and give up
Thing excludes, and accelerates metabolism, and sodium carbonate is changed into small-molecule substance by macromolecular complex in filter residue, makes filter residue absorption more the same
Material, while alginic acid is adsorbed, wrapped up by alginic acid, increase satiety, the present invention can not only reach the effect of degreasing, simultaneously
It is also very high in the absence of certain side effect, or even nutritional ingredient.
Embodiment
Chinese herbal medicine fluid preparation method：
A. by the Fen ﹑ hawthorn 2 ~ 3 of 2 ~ 3 Fen ﹑ malt of green tea 2 ~ 3 Fen ﹑ light-coloured vinegars, 1 ~ 2 Fen ﹑ radix pseudostellariaes, 2 ~ 3 Fen ﹑ dried orange peels, 2 ~ 3 Fen ﹑ Chinese yams 2 ~ 3
2 ~ 3 parts of 3 ~ 4 parts of ﹑ matrimony vines of thin ginseng are put into container on part ﹑ iron mountain, are poured into 8 ~ 10 parts of water and are soaked 2 ~ 3h；
B. immersion medicinal material being placed on container, heating is secondary, for the first time 60 ~ 80 DEG C 45 ~ 60min of ﹑, second 30 ~ 40 DEG C ﹑ 25 ~
30min, room temperature is cooled to, filters, obtain filtrate, i.e. Chinese herbal medicine fluid.
The preparation method of yeast fermentation broth：
A. count in parts by weight, take 8 ~ 10 parts of water, 20 ~ 30 parts of peptones, 10 ~ 15 parts of sodium chloride, 4 ~ 10 parts of agar, mixing is
It is even, high-temperature sterilization, make solid medium；
B. by resistance to high sugar yeast bacterium inoculation medium inclined-plane, 2 ~ 4d is cultivated at 27 ~ 29 DEG C of temperature, obtains slant strains, it is standby；
C. count in parts by weight, take 70 ~ 80 parts of water, 20 ~ 30 parts of peptones, 10 ~ 15 parts of sodium chloride, 4 ~ 10 parts of agar, mixing is
It is even, high-temperature sterilization, make liquid medium；
D. slant strains standby in step b are directly forwarded in nutrient solution, at 27 ~ 29 DEG C of temperature, cultivate 2 ~ 4d, prepare
Obtain yeast fermentation broth.
The preparation method of present invention fat-reducing ferment, comprises the following steps：
（1）According to the mass fraction, following raw material is taken, 2 ~ 3 parts of 2 ~ 3 parts of Ping Guo ﹑, 4 ~ 5 parts of Fan Qie ﹑ citrus You Guo ﹑, 5 ~ 6 parts of Ti ﹑ 2 ~
3 portions of lemons are put into NaCl solution, soak 2 ~ 3h, the raw material after must soaking, then water are poured into the raw material after immersion, cleaning,
Raw material is cut into slices again, is dried at 40 ~ 50 DEG C, takes drying object；
（2）Drying object is placed 10 ~ 12h is soaked in Chinese herbal medicine fluid, filtering, obtain filter residue；
（3）In mass ratio 1：3~4：2 ~ 3, by Lv Zha ﹑ NaCO3Rong Ye ﹑ alginic acids are stirred, be well mixed after heating 20 ~
30min, room temperature is cooled to, adds the bacterium solution of filter residue quality 15 ~ 18%, fermented, obtain fermenting mixture, zymotic fluid is entered
Row vacuum freeze drying, freeze-drying thing is collected, produces fat-reducing ferment.
Example 1
Chinese herbal medicine fluid preparation method：
A. by the thin ginseng 2 in 2 Fen ﹑ iron mountains of green tea 2 Fen ﹑ light-coloured vinegars, 1 Fen ﹑ radix pseudostellariaes, 2 Fen ﹑ dried orange peels, 2 Fen ﹑ Chinese yams, 2 Fen ﹑ malt, 2 Fen ﹑ hawthorn
3 parts of part ﹑ matrimony vines are put into container, pour into 8 parts of water immersion 2h；
B. being placed on container immersion medicinal material, heating is secondary, for the first time 60 DEG C ﹑ 45min, second of 30 DEG C ﹑ 25min, cooling
To room temperature, filtering, filtrate, i.e. Chinese herbal medicine fluid are obtained.
The preparation method of yeast fermentation broth：
A. count in parts by weight, take 8 parts of water, 20 parts of peptones, 10 parts of sodium chloride, 4 parts of agar, be well mixed, high-temperature sterilization, system
Make solid medium；
B. by resistance to high sugar yeast bacterium inoculation medium inclined-plane, 2d is cultivated at 27 DEG C of temperature, obtains slant strains, it is standby；
C. count in parts by weight, take 70 parts of water, 20 parts of peptones, 10 parts of sodium chloride, 4 parts of agar, be well mixed, high-temperature sterilization,
Make liquid medium；
D. slant strains standby in step b are directly forwarded in nutrient solution, at 27 DEG C of temperature, cultivate 2d, be prepared
Yeast fermentation broth.
The preparation method of present invention fat-reducing ferment, comprises the following steps：
（1）According to the mass fraction, following raw material is taken, 2 portions of 2 portions of Ping Guo ﹑, 4 portions of Fan Qie ﹑ citrus You Guo 5 portions of ﹑, 2 portions of Ti ﹑ lemons are put
Enter in NaCl solution, soak 2h, the raw material after must soaking, then water is poured into the raw material after immersion, cleaning, then raw material is cut into
Piece, dried at 40 DEG C, take drying object；
（2）Drying object is placed in Chinese herbal medicine fluid and soaks 10h, filters, obtains filter residue；
（3）In mass ratio 1：3：2, by Lv Zha ﹑ NaCO3Rong Ye ﹑ alginic acids are stirred, and 20min is heated after well mixed, are cooled down
To room temperature, the bacterium solution of filter residue quality 15% is added, is fermented, obtains fermenting mixture, zymotic fluid progress vacuum refrigeration is done
It is dry, freeze-drying thing is collected, produces fat-reducing ferment.
Example 2
Chinese herbal medicine fluid preparation method：
A. by the Fen ﹑ hawthorn 2.5 of 2.5 Fen ﹑ malt of green tea 2.5 Fen ﹑ light-coloured vinegars, 1.5 Fen ﹑ radix pseudostellariaes, 2.5 Fen ﹑ dried orange peels, 2.5 Fen ﹑ Chinese yams 2.5
2.5 parts of 3.5 parts of ﹑ matrimony vines of thin ginseng are put into container on part ﹑ iron mountain, pour into 9 parts of water immersion 2.5h；
B. immersion medicinal material is placed on container, heating is secondary, for the first time 70 DEG C ﹑ 52min, second of 35 DEG C ﹑ 27min, is cooled to
Room temperature, filtering, obtains filtrate, i.e. Chinese herbal medicine fluid.
The preparation method of yeast fermentation broth：
A. count in parts by weight, take 9 parts of water, 25 parts of peptones, 13 parts of sodium chloride, 7 parts of agar, be well mixed, high-temperature sterilization, system
Make solid medium；
B. by resistance to high sugar yeast bacterium inoculation medium inclined-plane, 3d is cultivated at 28 DEG C of temperature, obtains slant strains, it is standby；
C. count in parts by weight, take 75 parts of water, 25 parts of peptones, 13 parts of sodium chloride, 7 parts of agar, be well mixed, high-temperature sterilization,
Make liquid medium；
D. slant strains standby in step b are directly forwarded in nutrient solution, at 28 DEG C of temperature, cultivate 3d, be prepared
Yeast fermentation broth.
The preparation method of present invention fat-reducing ferment, comprises the following steps：
（1）According to the mass fraction, following raw material is taken, 2 portions of 2 portions of Ping Guo ﹑, 5 portions of Fan Qie ﹑ citrus You Guo 6 portions of ﹑, 2 portions of Ti ﹑ lemons are put
Enter in NaCl solution, soak 2h, the raw material after must soaking, then water is poured into the raw material after immersion, cleaning, then raw material is cut into
Piece, dried at 45 DEG C, take drying object；
（2）Drying object is placed in Chinese herbal medicine fluid and soaks 11h, filters, obtains filter residue；
（3）In mass ratio 1：3：2.5, by Lv Zha ﹑ NaCO3Rong Ye ﹑ alginic acids are stirred, and 25min is heated after well mixed, cold
But to room temperature, the bacterium solution of filter residue quality 16% is added, is fermented, obtains fermenting mixture, zymotic fluid progress vacuum refrigeration is done
It is dry, freeze-drying thing is collected, produces fat-reducing ferment.
Example 3
Chinese herbal medicine fluid preparation method：
A. by the thin ginseng 3 in 3 Fen ﹑ iron mountains of green tea 3 Fen ﹑ light-coloured vinegars, 2 Fen ﹑ radix pseudostellariaes, 3 Fen ﹑ dried orange peels, 3 Fen ﹑ Chinese yams, 3 Fen ﹑ malt, 3 Fen ﹑ hawthorn
4 parts of part ﹑ matrimony vines are put into container, pour into 10 parts of water immersion 3h；
B. immersion medicinal material is placed on container, heating is secondary, for the first time 80 DEG C ﹑ 60min, second of 40 DEG C ﹑ 30min, is cooled to
Room temperature, filtering, obtains filtrate, i.e. Chinese herbal medicine fluid.
The preparation method of yeast fermentation broth：
A. count in parts by weight, take 10 parts of water, 30 parts of peptones, 15 parts of sodium chloride, 10 parts of agar, be well mixed, high-temperature sterilization,
Make solid medium；
B. by resistance to high sugar yeast bacterium inoculation medium inclined-plane, 4d is cultivated at 29 DEG C of temperature, obtains slant strains, it is standby；
C. count in parts by weight, take 80 parts of water, 30 parts of peptones, 15 parts of sodium chloride, 10 parts of agar, be well mixed, high-temperature sterilization,
Make liquid medium；
D. slant strains standby in step b are directly forwarded in nutrient solution, at 29 DEG C of temperature, cultivate 4d, be prepared
Yeast fermentation broth.
The preparation method of present invention fat-reducing ferment, comprises the following steps：
（1）According to the mass fraction, following raw material is taken, 3 portions of 3 portions of Ping Guo ﹑, 5 portions of Fan Qie ﹑ citrus You Guo 6 portions of ﹑, 3 portions of Ti ﹑ lemons are put
Enter in NaCl solution, soak 3h, the raw material after must soaking, then water is poured into the raw material after immersion, cleaning, then raw material is cut into
Piece, dried at 50 DEG C, take drying object；
（2）Drying object is placed in Chinese herbal medicine fluid and soaks 12h, filters, obtains filter residue；
（3）In mass ratio 1：4：3, by Lv Zha ﹑ NaCO3Rong Ye ﹑ alginic acids are stirred, and 30min is heated after well mixed, are cooled down
To room temperature, the bacterium solution of filter residue quality 18% is added, is fermented, obtains fermenting mixture, zymotic fluid progress vacuum refrigeration is done
It is dry, freeze-drying thing is collected, produces fat-reducing ferment.
Reference examples：The fat-reducing ferment of company of Yichang City production
Fat-reducing ferment obtained by above-described embodiment and the fat-reducing ferment of reference examples are detected, specific detection is as follows：
Obesity crowd is selected to be randomly divided into 3 experimental groups and 1 by the body weight of the experimenter, body fat weight as study subject
Control group, experimental group recommend method to take the given the test agent of embodiment 1 ~ 3 by human body, and control group takes the subtracting on the market of equivalent
Fertile ferment, continuous 60 days, normal diet, work and rest during experiment.The body weight of human subject is determined after off-test, body fat contains
Amount.Subject's health status is unaffected before and after experiment, and internal trace element meets standard.Table 1 is result of the test statistical form.
Table 1：
| Test event | Example 1 | Example 2 | Example 3 | Reference examples |
| Body weight before experiment（kg） | 132 | 130 | 135 | 131~135 |
| Body weight after experiment（kg） | 110 | 112 | 108 | 120~123 |
| Body fat rate before experiment（%） | 32 | 30 | 33 | 31~33 |
| Body fat rate after experiment（%） | 20 | 23 | 22 | 30~31 |
In summary, fat-reducing ferment effect of the invention is more preferable, is worth of widely use.
Claims (4)
1. a kind of preparation method for ferment of losing weight, it is characterised in that the preparation method comprises the following steps：
（1）According to the mass fraction, following raw material is taken, 2 ~ 3 parts of 2 ~ 3 parts of Ping Guo ﹑, 4 ~ 5 parts of Fan Qie ﹑ citrus You Guo ﹑, 5 ~ 6 parts of Ti ﹑ 2 ~
3 portions of lemons are put into NaCl solution, soak 2 ~ 3h, the raw material after must soaking, then water are poured into the raw material after immersion, cleaning,
Raw material is cut into slices again, is dried at 40 ~ 50 DEG C, takes drying object；
（2）Drying object is placed 10 ~ 12h is soaked in Chinese herbal medicine fluid, filtering, obtain filter residue；
（3）In mass ratio 1：3~4：2 ~ 3, by Lv Zha ﹑ NaCO3Rong Ye ﹑ alginic acids are stirred, be well mixed after heating 20 ~
30min, room temperature is cooled to, adds the bacterium solution of filter residue quality 15 ~ 18%, fermented, obtain fermenting mixture, zymotic fluid is entered
Row vacuum freeze drying, freeze-drying thing is collected, produces fat-reducing ferment.
2. the preparation method for ferment of losing weight according to claim 1, it is characterised in that the step（2）Middle Chinese herbal medicine fluid is
Include the raw material of following parts by weight, the Fen ﹑ wheats of 2 ~ 3 Fen ﹑ Chinese yams of green tea 2 ~ 3 Fen ﹑ light-coloured vinegars, 1 ~ 2 Fen ﹑ radix pseudostellariaes, 2 ~ 3 Fen ﹑ dried orange peels 2 ~ 3
Thin ginseng 2 ~ 3 Fen ﹑ matrimony vines, 3 ~ 4 Fen ﹑, 8 ~ 10 parts of water in the Fen ﹑ iron mountain of 2 ~ 3 Fen ﹑ hawthorn of bud 2 ~ 3.
3. the preparation method for ferment of losing weight according to claim 1, it is characterised in that the step（3）Middle bacterium solution is yeast
Zymotic fluid, the preparation method of yeast fermentation broth：
A. count in parts by weight, take 8 ~ 10 parts of water, 20 ~ 30 parts of peptones, 10 ~ 15 parts of sodium chloride, 4 ~ 10 parts of agar, mixing is
It is even, high-temperature sterilization, make solid medium；
B. by resistance to high sugar yeast bacterium inoculation medium inclined-plane, 2 ~ 4d is cultivated at 27 ~ 29 DEG C of temperature, obtains slant strains, it is standby；
C. count in parts by weight, take 70 ~ 80 parts of water, 20 ~ 30 parts of peptones, 10 ~ 15 parts of sodium chloride, 4 ~ 10 parts of agar, mixing is
It is even, high-temperature sterilization, make liquid medium；
D. slant strains standby in step b are directly forwarded in nutrient solution, at 27 ~ 29 DEG C of temperature, cultivate 2 ~ 4d, prepare
Obtain yeast fermentation broth.
4. the preparation method for ferment of losing weight according to claim 1, it is characterised in that the step（3）Middle vacuum drying is
Temperature -15 ~ -10 DEG C, 1.3 ~ 13Pa of vacuum.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108783152A (en) * | 2018-05-30 | 2018-11-13 | 广州雅惠生物科技有限公司 | Composite enzyme beverage with effect of weight reducing and preparation method thereof |
| CN108936624A (en) * | 2018-07-30 | 2018-12-07 | 山东晨彰生物科技有限公司 | A kind of ferment and preparation method thereof with weight losing function |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108783152A (en) * | 2018-05-30 | 2018-11-13 | 广州雅惠生物科技有限公司 | Composite enzyme beverage with effect of weight reducing and preparation method thereof |
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