CN104258106B - Preparation method of effective part of dendrobium officinale - Google Patents

Preparation method of effective part of dendrobium officinale Download PDF

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CN104258106B
CN104258106B CN201410542193.3A CN201410542193A CN104258106B CN 104258106 B CN104258106 B CN 104258106B CN 201410542193 A CN201410542193 A CN 201410542193A CN 104258106 B CN104258106 B CN 104258106B
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陈贤良
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Zhejiang Maple Kang group Co., Ltd.
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NINGBO FENGKANG BIOLOGICAL TECHNOLOGY Co Ltd
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Abstract

The invention discloses a preparation method of an effective part of dendrobium officinale. The method comprises steps as follows: firstly, extracting fat-soluble extracts represented by bibenzyl from the dendrobium officinale with a high-concentration alcohol-water system, refining the extracts with a macroporous resin method to obtain a total bibenzyl effective part with the bibenzyl content higher than 50%, and preparing dregs of the extracts into dendrobium officinale total polysaccharides with water extraction and alcohol precipitation methods; drying and evenly mixing the total bibenzyl effective part and the dendrobium officinale total polysaccharides obtained in two steps to obtain a dendrobium officinale total effective part extract. According to the method, the defect of the extremely low extraction rate of dendrobium officinale fat-soluble active ingredients represented by the bibenzyl with traditional methods such as a water decoction method, a tea making method and the like is overcome, the utilization efficiency of the effective part of the dendrobium officinale is improved substantially, pharmacological anti-oxidation animal experiments and anti-tumor cytotoxicity experiments prove that the activity of the effective part is significantly higher than that of traditional aqueous extracts, and the effective part has the characteristics of high extraction rate and high activity.

Description

A kind of preparation method of dendrobium candidum active component
Technical field
The present invention relates to pharmaceutical technology field, specifically a kind of preparation method of dendrobium candidum active component.
Background technology
Dendrobium candidum is orchid family Dendrobium herbaceos perennial, and its main medicinal part is fresh or is dried stem, mainly The ground such as Anhui, Zhejiang, Guangxi, Hunan, Yunnan, Guizhou are distributed in, are traditional rare traditional Chinese medicine.Dendrobium candidum has beneficial stomach life The effect of Tianjin, nourishing Yin and clearing heat, for impairment of yin body fluid deficiency, dry polydipsia, deficiency of stomach-Yin, deficiency of food is retched, and after being ill abnormal heat is not moved back, deficiency of Yin fire Prosperous, hectic fever due to yin labor heat, mesh is secretly failed to understand, the disease such as muscles and bones impotence.《Sheng Nong's herbal classic》Top grade is classified as, Taoism regimen is classical《Taoist Scriptures》 It is described as first of " Chinese nine big celestial grass ".
Water miscible polysaccharide, fat-soluble bibenzyl are mainly contained in dendrobium candidum, also containing a small amount of water miscible ammonia Base is sour, water miscible micro-, water miscible phenolic acid class, and micro partially fat-soluble stem of noble dendrobium alkaloid, fat-soluble Stem of noble dendrobium sequiterpene, partially fat-soluble fluorenes ketone compounds, partially fat-soluble lignanoid, water miscible phenols, partially fat-soluble Huang Ketone, fat-soluble steroidal etc., wherein bibenzyl have the valuable pharmacological active function such as antitumor, anti-oxidant.Traditional iron sheet stone Dry measure used in former times Related product, including various little complex health care products, make tea maple bucket, polysaccharide, its technique is water extraction or water extraction concentrated extract, this Planting traditional water extraction process obviously can not play the Bibenzyl compound of the valuable pharmacological active function such as antitumor, anti-oxidant Extract well with other liposoluble constituents, cause extensive Product Process, weak curative effect, serious waste of resources.
The content of the invention
It is an object of the invention to provide a kind of preparation method of dendrobium candidum active component, substantially increase total starches and The recovery rate of total bibenzyl.
For achieving the above object, the present invention provides following technical scheme：
A kind of preparation method of dendrobium candidum active component, including the extraction of fat-soluble total bibenzyl and water-soluble total starches Extract.
As further scheme of the invention：The extraction step of fat-soluble total bibenzyl is：Dendrobium candidum is pressed into solid-liquid Than adding the 8-12 times of high concentration ethanol aqueous solution for measuring 65%-95%, 70-100 DEG C is heated to, refluxing extraction 2-3 time, each 2- 3h；Merge extract, reduced pressure concentration obtains fat-soluble extract；The extract is dissolved with ethanol in proper amount, macroporous resin column is then added to On, the ethanol water wash-out part of its 25%-75% is taken, it is concentrated to give total bibenzyl active component；The water-soluble total starches Extraction step be：The remaining dregs of a decoction of extraction step with fat-soluble total bibenzyl add 8-12 times to measure water as raw material, and 70-100 DEG C adds Circumfluence distillation 2-3 time, each 2-3h is concentrated into the volume of 1/10-1/12, adds the 4-6 times of ethanol for measuring 95% to carry out alcohol precipitation, Filter, drying and crushing obtains total starches；Total bibenzyl active component is uniformly mixed with total starches xeraphium, obtaining dendrobium candidum always has Effect extractive part.
As further scheme of the invention：The extraction step of fat-soluble total bibenzyl is：Dendrobium candidum is pressed into solid-liquid Than the ethanol waters for adding 12 times of amounts 80%, 92 DEG C are heated to, refluxing extraction 2 times, each 3h；Merge extract, reduce pressure dense Contract to obtain fat-soluble extract；The extract is dissolved with ethanol in proper amount, is then added in D101 macroporous resin columns, take its 40%-60% Ethanol water wash-out part, be concentrated to give total bibenzyl active component.
Compared with prior art, the invention has the beneficial effects as follows：The mode such as traditional water decoction is overcome, makes tea for bibenzyl For the extremely low shortcoming of the dendrobium candidum fat-soluble active ingredient recovery rate for representing, the profit of dendrobium candidum active ingredient greatly improved Tested with efficiency, and the anti-oxidant zoopery of Jing pharmacology and antitumor cell poison, it was demonstrated that its activity has than traditional water extract Significantly improve, have high extraction, highly active feature concurrently.
Description of the drawings
Fig. 1 is the HPLC-DAD chromatograms of dendrobium candidum fat-soluble extract, and wavelength is 280nm；
Fig. 2 is the HPLC-MS liquid chromatograms of dendrobium candidum fat-soluble extract, and wavelength is 280nm；
Fig. 3 be dendrobium candidum fat-soluble extract HPLC-MS liquid chromatograms in chromatographic peak 1 first mass spectrometric figure；
Fig. 4 be dendrobium candidum fat-soluble extract HPLC-MS liquid chromatograms in chromatographic peak 2 first mass spectrometric figure；
Fig. 5 be dendrobium candidum fat-soluble extract HPLC-MS liquid chromatograms in chromatographic peak 3 first mass spectrometric figure；
Fig. 6 be dendrobium candidum fat-soluble extract HPLC-MS liquid chromatograms in chromatographic peak 4 first mass spectrometric figure；
Fig. 7 be dendrobium candidum fat-soluble extract HPLC-MS liquid chromatograms in chromatographic peak 5 first mass spectrometric figure；
Fig. 8 be dendrobium candidum fat-soluble extract HPLC-MS liquid chromatograms in chromatographic peak 6 first mass spectrometric figure；
Fig. 9 be dendrobium candidum fat-soluble extract HPLC-MS liquid chromatograms in chromatographic peak 7 first mass spectrometric figure；
Figure 10 be dendrobium candidum fat-soluble extract HPLC-MS liquid chromatograms in chromatographic peak 8 first mass spectrometric figure.
Specific embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, Obviously, described embodiment is only a part of embodiment of the invention, rather than the embodiment of whole.Based in the present invention Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all Belong to the scope of protection of the invention.
In the embodiment of the present invention, a kind of preparation method of dendrobium candidum active component, including the extraction of fat-soluble total bibenzyl With the extraction of water-soluble total starches.The extraction step of fat-soluble total bibenzyl is：Dendrobium candidum is added into 8-12 by solid-to-liquid ratio The high concentration ethanol aqueous solution of 65%-95% times is measured, 70-100 DEG C is heated to, refluxing extraction 2-3 time, each 2-3h；Merging is carried Liquid is taken, reduced pressure concentration obtains fat-soluble extract；The extract is dissolved with ethanol in proper amount, is then added in macroporous resin column, take it The ethanol water wash-out part of 25%-75%, is concentrated to give total bibenzyl active component；The extraction step of the water-soluble total starches Suddenly it is：The remaining dregs of a decoction of extraction step with fat-soluble total bibenzyl add 8-12 times and measure water as raw material, and 70-100 DEG C is heated to reflux carrying Take 2-3 time, each 2-3h, be concentrated into the volume of 1/10-1/12, add the 4-6 times of ethanol for measuring 95% to carry out alcohol precipitation, filter, do It is dry to crush to obtain total starches；Total bibenzyl active component is uniformly mixed with total starches xeraphium, dendrobium candidum total effective parts are obtained Extract.
Total starches recovery rate is determined using standards of pharmacopoeia method alcohol precipitation-phenol-sulphuric acid colorimetry；With EN as control Product, using ultraviolet spectrophotometry total bibenzyl recovery rate is determined under 280nm.
1st, the content of total bibenzyl in fat-soluble extract is detected using following two modes
(1) Bibenzyl compound in HPLC-PAD methods detection fat-soluble extract
Method：4ml extracts 10000r centrifugation 10min are taken out, supernatant is redissolved with methyl alcohol after being evaporated and is then transferred to automatically In sample injection bottle, detected by HPLC-PAD.
Instrument：Waters 2695-2996Photodiode Array Detector high performance liquid chromatographs.
Chromatographic condition：COSMOSIL Packed ODS chromatographic columns (250mm × 4.6mm)；25 DEG C of column temperature；Detection wavelength 280nm；Mobile phase is：The formic acid of methyl alcohol -0.1% (20:80)；Flow velocity is 0.8ml/min.
As a result：See accompanying drawing 1 and table 1.Chromatogram peak list and PAD UV scanning results in Fig. 1, and fat-soluble extract With the ultraviolet data of EN reference substance：
Conclusion：With reference to bibenzyl UV characteristic absorption peaks in 280nm, 220nm or so, illustrate in the fat-soluble extract containing big The compound with bibenzyl UV characteristic absorption peaks of amount.
Table 1PAD UV scanning results
Retention time Peak area Peak area percent Ultraviolet maximum absorption band/nm
1 2.519 59033 0.34 294
2 2.863 1443223 8.37 288
3 3.164 372468 2.16 278(shoulder peak)
4 3.518 1688226 9.79 271
5 3.862 883337 5.12 257
6 4.067 129686 0.75 275(shoulder peak)
7 4.249 2179418 12.64 280
8 4.699 952448 5.52 295
9 5.247 4531132 26.28 287
10 5.636 2886638 16.74 284
11 7.143 157225 0.91 300
12 7.825 87928 0.51 276,220
13 8.068 54018 0.31 280,218
14 8.716 190555 1.11 285
15 9.474 742223 4.31 276
16 11.72 78187 0.45 293
17 12.622 106621 0.62 300
18 13.871 78581 0.46 259,206
19 14.967 24184 0.14 283,253
20 15.333 109394 0.63 305,210
21 17.1 44359 0.26 285,223
Continued 1PAD UV scanning result
Retention time Peak area Peak area percent Ultraviolet maximum absorption band/nm
22 17.483 82995 0.48 293,234
23 18.755 165076 0.96 291,248
24 21.6 80513 0.47 281,228
25 23.357 112784 0.65 283,222
Total position 278,220
EN 280,218
(2) Bibenzyl compound in HPLC-MS methods detection fat-soluble extract
Method：4ml extracts 10000r centrifugation 10min are taken out, supernatant is redissolved with methyl alcohol after being evaporated and is then transferred to automatically In sample injection bottle, detected by HPLC-MS.
Instrument：Liquid chromatogram-G6200 series of time of flight the GC-MSs of Aglient 1290.
Chromatographic condition：Methanol-water (30:70)；Flow velocity is 0.8ml/min.
As a result：As shown in figs. 2-10.
Conclusion：In HPLC-MS liquid chromatograms, there is the characteristic molecular of bibenzyl series in the first mass spectrometric figure of main chromatographic peak Amount, such as mass-to-charge ratio m/z be 304 (Fig. 3), 288 (Fig. 4), 342 (Fig. 5), 198 (Fig. 6), 278 (Fig. 7-Fig. 8), 378 (Fig. 9), 420 (Figure 10) etc..It can be seen that containing a large amount of Bibenzyl compounds in the extract.
2nd, the optimization of orthogonal test of fat-soluble extract extraction conditions
Select to affect larger concentration of alcohol, three factors of extraction time and extraction time to carry out just bibenzyl recovery rate Hand over design experiment, with bibenzyl recovery rate as index, preferably optimal technological parameter, it is determined that with ethanol as solvent extraction bibenzyl most Good extraction process.Factor level arranges such as table 2.
Concretely comprise the following steps：Dendrobium candidum 1g is weighed, circumfluence method extraction is carried out, fixed heating-up temperature is 92 DEG C, concentration of alcohol For 80%, ultraviolet spectrophotometry method determines total bibenzyl content.Orthogonal experiments and analysis in table 3- table 4.
Orthogonal experiment results show that the influence degree of each factor is followed successively by B>C>A, i.e. extraction time>Extraction time>Ethanol Concentration, optimum extraction process is B3C3A3, i.e., 80% ethanol water, refluxing extraction 3 times, each 3h.
The orthogonal test factor level table of table 2
The bibenzyl recovery rate result of the orthogonal test of table 3
The variance analysis of the orthogonal test of table 4
Checking test：
Weigh dendrobium candidum 1g, add the ethanol water of 12ml80%, soaked overnight, 92 DEG C of heating and refluxing extractions 2 times, Let cool, in being transferred to 25ml volumetric flasks, with a small amount of moisture time washing container, washing lotion is incorporated in same volumetric flask, adds water to scale, Shake up, take 4ml centrifugations 15min therein, rotating speed is 10000r/min, and precision measures supernatant and dilutes 20 times, surveys at 280nm A is obtained, then C is read from regression equation, calculate bibenzyl recovery rate.As a result such as table 5：The optimum extraction rate of Bibenzyl compound For 3.25%, on the basis of recovery rate does not have significant difference, for energy saving, second extracting method can be selected, 92 DEG C of extractions of ethanol water of the 80% of i.e. 12 times amounts, extract 2 times, each 3h, extraction rate reached to 3.11%.
Bibenzyl recovery rate in the checking test of table 5
3rd, control group one：The recovery rate of total starches and total bibenzyl in the method that tradition brews up
1) repeatedly make tea experiment：Dendrobium candidum 6g is weighed, boiling water 300ml is added, is soaked once every 2h, last time is soaked Bubble is overnight.As a result as shown in table 6, it is 1.50% that total starches add with total recovery rate, and it is 1.72% that total bibenzyl adds with total recovery rate.
2) single long-time heat preservation bottle/thermos-cooker immersion test：Dendrobium candidum 3g is weighed, in being put into vacuum flask/thermos-cooker, Boiling water 150ml, sealed thermal insulating immersion 10h are added, the recovery rate of total starches is 1.19%, the recovery rate of total bibenzyl is 1.37%.
The total starches of more than 6 experiment of making tea of table and the recovery rate of total bibenzyl
4th, control group two：In traditional extraction process by water in dendrobium candidum total bibenzyl recovery rate.
The total starches and the recovery rate of total bibenzyl of the traditional extraction process by water of table 7
Dendrobium candidum 1g is weighed, plus 100 DEG C of the water of 12 times of amounts is extracted 2 times, each 2h.As a result it is as shown in table 7：Total starches Average recoveries are 5.98%, and the average recoveries of total bibenzyl are 1.74%.
In sum：Three kinds of different extracting modes of contrast, traditional makes tea in method, repeatedly brews adduction recovery rate and one Secondary long-time brews recovery rate quite, but the latter, due to a taking dose height, possible drug effect is more preferable；Traditional extraction process by water with Brew that mode is the same, it is relatively not high for the recovery rate of liposoluble constituent；The present invention is by the alcohol extracting of high concentration, hence it is evident that improve The recovery rate of Bibenzyl compound.
Embodiment 1
The preparation of total bibenzyl active component
It is dried dendrobium candidum 1kg, adds the ethanol of 12L80%, soaked overnight, 92 DEG C of heating and refluxing extractions 2 times, every time 3h, merges extract, and recycling design extremely obtains 98g medicinal extract and is dried without alcohol taste, as fat-soluble extract；With the 95% of 30ml Ethanol dissolving, then be put on D101 type macroreticular resins, take 40%-60% wash-outs position, reduced pressure concentration is dried, and obtaining total bibenzyl has Effect position 46g, it is 60.5% that ultraviolet spectrophotometry measures the bibenzyl content in active component.
Embodiment 2
The preparation of dendrobium candidum total effective parts
The Chinese medicine slag of Example 1 by the water that water extraction and alcohol precipitation method prepares total starches, i.e. 12L be heated to 100 DEG C extract 2 times, often Secondary 2h；Extract is concentrated into into 1/12 volume 2L, adds the ethanol of 10L 95%, precipitate polysaccharides to filter, be dried, obtain total starches 42g, takes above-mentioned total bibenzyl 23g, merges with total starches 21g, total effective parts 44g is obtained, equivalent to the amount of crude drug 500g.
Embodiment 3
Traditional water extract, total effective parts, the activity research of total bibenzyl active component
3.1 anti-oxidant zooperies
Animal：Kunming mouse, is provided by Zhejiang University's Experimental Animal Center.18 months mouse ages, 45～55g of body weight, totally 40 Only, male and female half and half.
Male and female mouse is divided at random respectively 4 groups：Normal aged mouse control group；Traditional water extract group；Total effectively portion Hyte；Total bibenzyl active component group.
Daily gavage 2 times, each 50mg, continuous 30d.2h after last dose, sacrificed by decapitation mouse takes blood, separated plasma And red blood cell；Hepatic tissue, nephridial tissue and ventricular homogenate are separately taken, by thio barbital method Plasma MDA (MDA) is determined, pressed EistnerFF methods determine the activity of erythrocyte superoxide dismutase (SOD), and experimental result is as shown in table 8.
Impact (* P of the dendrobium candidum extract of table 8 to Antioxidation Effect<0.01)
Conclusion：Traditional water extract is compared, total effective parts and total bibenzyl active component can substantially increase antioxidation Drug effect.
The poison experiment of 3.2 antitumor cells
Suppression of the observation candidate compound to human lung carcinoma cell 95-D and the in-vitro multiplication ability of Proliferation of Human Ovarian Cell A2780 Effect.
Method：Sulforhodamine B (SRB) method detects that candidate compound increases to the 95-D and A2780 in exponential phase Grow the inhibitory action of ability.
Experiment material：Human lung carcinoma cell line 95-D and HOC are used in tested extract, tetrazolium bromide SRB (Sigma), experiment Cell A2780.95-D and A2780 are incubated at respectively RPMI-1640 and DMEM (GIBCO, Grand Island, NY, U.S.A) contains The hyclone of 10% inactivation, Glu (GIBCO) 2nmol/L, penicillin 100IU/mL and the μ g/mL of streptomysin 100.
Concrete steps：(1) cell line is adopted：Human lung carcinoma cell 95-D and Proliferation of Human Ovarian Cell A2780；(2) logarithm is given birth to After long-term cell dissociation, single cell suspension is blown and beaten into, be inoculated in 96 well culture plates, 5x103Cells/well, per hole culture medium 100 μ L, in 37 DEG C, 5% CO2Overnight incubation in incubator；(3) after cell attachment, the tested extract for adding debita spissitudo exists 3 days are further cultured in incubator；(4) trichloroacetic acid for adding 10% fixes 1h；(5) washed with distilled water, after being dried, added per hole The SRB solution of 100 μ L4mg/mL, room temperature is dyeed 20 minutes, is washed with 1% acetic acid, is dried；(6) add 100 μ L's per hole Trishydroxymethylaminomethane (Tris) solution of 10mM dissolves SRB；(7) ELIASA detects each hole OD values, and Detection wavelength is 515nm；Record result；Inhibiting rate is calculated by following equation：Inhibiting rate (%)=(OD control-OD administrations)/OD controls × 100%, and calculate IC50.If the IC of tested material50It is less to show that activity is better.
Experimental result is as shown in table 9：
The dendrobium candidum extract Cyto toxic experiment showed (IC of table 950, μ g/ml)
Interpretation of result：Tested extract has different journeys to human lung carcinoma cell 95-D and Proliferation of Human Ovarian Cell A2780 propagation The inhibitory action of degree, wherein total effective parts and the more traditional water extract drug effect of total bibenzyl active component are significantly improved.
Embodiment 4
The preparation of total effective parts, total bibenzyl effective parts formulation
4.1 total effective parts make tablet
The gained total effective parts 25g of embodiment 1 and starch 15g is mixed, plus 10% starch slurry 5g makes softwood, adds hard Fatty acid magnesium 0.25g, dried starch 4g are pressed into 120 after mixing, and obtain final product.Per piece 208mg containing total effective parts.
4.2 total effective parts make capsule
Add 10% starch slurry to make softwood the full component extract 10g of the gained of embodiment 1, after being pelletized with 14 mesh sieves, put 60 DEG C～70 DEG C of dryings after 12 mesh sieve whole grains, in being inserted in No. 1 Capsules.Grain 500 is filled altogether, and every capsule contains total effective parts 20mg。
4.3 total bibenzyl active components make capsule
By the total bibenzyl active component 10g of the gained of embodiment 2,12 mesh sieves are crossed after 60 DEG C～70 DEG C dryings, be inserted in No. 1 it is hollow In capsule.Grain 200 is filled altogether, and every capsule contains total bibenzyl active component 50mg.
Instant invention overcomes traditional water decoction, the mode such as make tea for the dendrobium candidum liposoluble active with bibenzyl as representative into The shortcoming for dividing recovery rate extremely low, greatly improved the utilization ratio of dendrobium candidum active ingredient, and the anti-oxidant animal of Jing pharmacology Test and antitumor cell poison experiment, it was demonstrated that its activity, with significantly improving, has high extraction, high activity concurrently than traditional water extract The characteristics of.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie In the case of spirit or essential attributes without departing substantially from the present invention, the present invention can be in other specific forms realized.Therefore, no matter From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power Profit is required rather than described above is limited, it is intended that all in the implication and scope of the equivalency of claim by falling Change is included in the present invention.
Moreover, it will be appreciated that although this specification is been described by according to embodiment, not each embodiment is only wrapped Containing an independent technical scheme, this narrating mode of specification is only that for clarity those skilled in the art should Using specification as an entirety, the technical scheme in each embodiment can also Jing it is appropriately combined, form those skilled in the art Understandable other embodiment.

Claims (2)

1. a kind of preparation method of dendrobium candidum active component, it is characterised in that extraction including fat-soluble total bibenzyl and water-soluble The extraction of property total starches；The extraction step of fat-soluble total bibenzyl is：Dendrobium candidum is added into 8-12 times by solid-to-liquid ratio to measure The high concentration ethanol aqueous solution of 65%-95%, is heated to 70-100 DEG C, refluxing extraction 2-3 time, each 2-3h；Merge extract, Reduced pressure concentration obtains fat-soluble extract；The extract is dissolved with ethanol in proper amount, is then added in macroporous resin column, take its 25%- 75% ethanol water wash-out part, is concentrated to give total bibenzyl active component；The extraction step of the water-soluble total starches is： The remaining dregs of a decoction of extraction step with fat-soluble total bibenzyl add 8-12 times and measure water, 70-100 DEG C of heating and refluxing extraction 2-3 as raw material Secondary, each 2-3h is concentrated into the volume of 1/10-1/12, adds the 4-6 times of ethanol for measuring 95% to carry out alcohol precipitation, filters, drying and crushing Obtain total starches；Total bibenzyl active component is uniformly mixed with total starches xeraphium, dendrobium candidum total effective extractive part is obtained.
2. the preparation method of dendrobium candidum active component according to claim 1, it is characterised in that described fat-soluble total The extraction step of benzyl is：Dendrobium candidum is added into the ethanol water of 12 times of amounts 80% by solid-to-liquid ratio, 92 DEG C are heated to, backflow is carried Take 2 times, each 3h；Merge extract, reduced pressure concentration obtains fat-soluble extract；The extract is dissolved with ethanol in proper amount, is then added to In D101 macroporous resin columns, the ethanol water wash-out part of its 40%-60% is taken, be concentrated to give total bibenzyl active component.
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CN104719897A (en) * 2015-03-26 2015-06-24 宁波枫康生物科技有限公司 Formula of composite health dendrobium officinale food capable of enhancing immunity and preparation method of composite health dendrobium officinale food
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CN105085695A (en) * 2015-06-12 2015-11-25 浙江仙人草保健品有限公司 Preparation method for dendrobium candidum extract powder
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