CN108295102A - A kind of moringa oleifera leaf extractive and extracting method with Lipid-lowering activities - Google Patents
A kind of moringa oleifera leaf extractive and extracting method with Lipid-lowering activities Download PDFInfo
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- CN108295102A CN108295102A CN201810311389.XA CN201810311389A CN108295102A CN 108295102 A CN108295102 A CN 108295102A CN 201810311389 A CN201810311389 A CN 201810311389A CN 108295102 A CN108295102 A CN 108295102A
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- Prior art keywords
- moringa
- leaf
- ethyl alcohol
- extractive
- lipid
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- 235000011347 Moringa oleifera Nutrition 0.000 title claims abstract description 174
- 244000179886 Moringa oleifera Species 0.000 title claims abstract description 156
- 230000000694 effects Effects 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 14
- JMFSHKGXVSAJFY-UHFFFAOYSA-N Saponaretin Natural products OCC(O)C1OC(Oc2c(O)cc(O)c3C(=O)C=C(Oc23)c4ccc(O)cc4)C(O)C1O JMFSHKGXVSAJFY-UHFFFAOYSA-N 0.000 claims abstract description 25
- MOZJVOCOKZLBQB-UHFFFAOYSA-N Vitexin Natural products OCC1OC(Oc2c(O)c(O)cc3C(=O)C=C(Oc23)c4ccc(O)cc4)C(O)C(O)C1O MOZJVOCOKZLBQB-UHFFFAOYSA-N 0.000 claims abstract description 25
- 229930003944 flavone Natural products 0.000 claims abstract description 17
- 235000011949 flavones Nutrition 0.000 claims abstract description 17
- SGEWCQFRYRRZDC-VPRICQMDSA-N vitexin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=C(O)C2=C1OC(C=1C=CC(O)=CC=1)=CC2=O SGEWCQFRYRRZDC-VPRICQMDSA-N 0.000 claims abstract description 15
- PZKISQRTNNHUGF-UHFFFAOYSA-N vitexine Natural products OC1C(O)C(O)C(CO)OC1OC1=C(O)C=C(O)C2=C1OC(C=1C=CC(O)=CC=1)=CC2=O PZKISQRTNNHUGF-UHFFFAOYSA-N 0.000 claims abstract description 15
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 claims abstract description 14
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 claims abstract description 14
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 claims abstract description 14
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 claims abstract description 14
- 229940074393 chlorogenic acid Drugs 0.000 claims abstract description 14
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 claims abstract description 14
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 claims abstract description 14
- 235000001368 chlorogenic acid Nutrition 0.000 claims abstract description 14
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 claims abstract description 14
- 150000002212 flavone derivatives Chemical class 0.000 claims abstract description 14
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- GXMWXESSGGEWEM-UHFFFAOYSA-N isoquercitrin Natural products OCC(O)C1OC(OC2C(Oc3cc(O)cc(O)c3C2=O)c4ccc(O)c(O)c4)C(O)C1O GXMWXESSGGEWEM-UHFFFAOYSA-N 0.000 claims abstract description 13
- OVSQVDMCBVZWGM-QSOFNFLRSA-N quercetin 3-O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C(C=2C=C(O)C(O)=CC=2)OC2=CC(O)=CC(O)=C2C1=O OVSQVDMCBVZWGM-QSOFNFLRSA-N 0.000 claims abstract description 13
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 12
- MQVRGDZCYDEQML-UHFFFAOYSA-N Astragalin Natural products C1=CC(OC)=CC=C1C1=C(OC2C(C(O)C(O)C(CO)O2)O)C(=O)C2=C(O)C=C(O)C=C2O1 MQVRGDZCYDEQML-UHFFFAOYSA-N 0.000 claims abstract description 11
- JPUKWEQWGBDDQB-QSOFNFLRSA-N kaempferol 3-O-beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C(C=2C=CC(O)=CC=2)OC2=CC(O)=CC(O)=C2C1=O JPUKWEQWGBDDQB-QSOFNFLRSA-N 0.000 claims abstract description 11
- MYXNWGACZJSMBT-VJXVFPJBSA-N isovitexin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=C(OC(=CC2=O)C=3C=CC(O)=CC=3)C2=C1O MYXNWGACZJSMBT-VJXVFPJBSA-N 0.000 claims abstract description 10
- OYJCWTROZCNWAA-UHFFFAOYSA-N isovitexin Natural products OCC1OC(C(O)C(O)C1O)c2c(O)cc3CC(=CC(=O)c3c2O)c4ccc(O)cc4 OYJCWTROZCNWAA-UHFFFAOYSA-N 0.000 claims abstract description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 109
- 235000019441 ethanol Nutrition 0.000 claims description 59
- 239000011347 resin Substances 0.000 claims description 38
- 229920005989 resin Polymers 0.000 claims description 38
- 239000007788 liquid Substances 0.000 claims description 32
- 239000000287 crude extract Substances 0.000 claims description 31
- 238000000605 extraction Methods 0.000 claims description 28
- 238000000746 purification Methods 0.000 claims description 25
- 239000000203 mixture Substances 0.000 claims description 24
- 238000010438 heat treatment Methods 0.000 claims description 23
- 239000000284 extract Substances 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 238000010992 reflux Methods 0.000 claims description 16
- 239000012488 sample solution Substances 0.000 claims description 13
- 238000011068 loading method Methods 0.000 claims description 11
- 239000000523 sample Substances 0.000 claims description 11
- 239000002904 solvent Substances 0.000 claims description 9
- 238000002791 soaking Methods 0.000 claims description 3
- 241000220215 Moringa Species 0.000 claims 18
- 238000011084 recovery Methods 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 42
- 238000001914 filtration Methods 0.000 description 14
- 239000003925 fat Substances 0.000 description 8
- 150000003626 triacylglycerols Chemical class 0.000 description 8
- 238000001035 drying Methods 0.000 description 7
- 239000003480 eluent Substances 0.000 description 7
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 238000004113 cell culture Methods 0.000 description 6
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- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
- 231100000240 steatosis hepatitis Toxicity 0.000 description 4
- RYMZZMVNJRMUDD-UHFFFAOYSA-N SJ000286063 Natural products C12C(OC(=O)C(C)(C)CC)CC(C)C=C2C=CC(C)C1CCC1CC(O)CC(=O)O1 RYMZZMVNJRMUDD-UHFFFAOYSA-N 0.000 description 3
- 239000004480 active ingredient Substances 0.000 description 3
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- 235000013305 food Nutrition 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- RYMZZMVNJRMUDD-HGQWONQESA-N simvastatin Chemical compound C([C@H]1[C@@H](C)C=CC2=C[C@H](C)C[C@@H]([C@H]12)OC(=O)C(C)(C)CC)C[C@@H]1C[C@@H](O)CC(=O)O1 RYMZZMVNJRMUDD-HGQWONQESA-N 0.000 description 3
- 229960002855 simvastatin Drugs 0.000 description 3
- 239000012085 test solution Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 239000008802 xuezhikang Substances 0.000 description 3
- UFLHIIWVXFIJGU-ARJAWSKDSA-N (Z)-hex-3-en-1-ol Chemical compound CC\C=C/CCO UFLHIIWVXFIJGU-ARJAWSKDSA-N 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 2
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- NPGIHFRTRXVWOY-UHFFFAOYSA-N Oil red O Chemical compound Cc1ccc(C)c(c1)N=Nc1cc(C)c(cc1C)N=Nc1c(O)ccc2ccccc12 NPGIHFRTRXVWOY-UHFFFAOYSA-N 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 239000012980 RPMI-1640 medium Substances 0.000 description 2
- DLGHDBFBVVPDCH-UHFFFAOYSA-L [OH-].[Na+].[N+](=O)([O-])[O-].[Al+3].N(=O)[O-].[Na+] Chemical compound [OH-].[Na+].[N+](=O)([O-])[O-].[Al+3].N(=O)[O-].[Na+] DLGHDBFBVVPDCH-UHFFFAOYSA-L 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
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- 238000007598 dipping method Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 2
- 239000012894 fetal calf serum Substances 0.000 description 2
- 235000019253 formic acid Nutrition 0.000 description 2
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 2
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- -1 polyphenol compounds Chemical class 0.000 description 2
- 230000003449 preventive effect Effects 0.000 description 2
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 2
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 2
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 2
- 235000005493 rutin Nutrition 0.000 description 2
- 229960004555 rutoside Drugs 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 2
- 102100029077 3-hydroxy-3-methylglutaryl-coenzyme A reductase Human genes 0.000 description 1
- 101710158485 3-hydroxy-3-methylglutaryl-coenzyme A reductase Proteins 0.000 description 1
- 101710168309 CCAAT/enhancer-binding protein alpha Proteins 0.000 description 1
- 102100034808 CCAAT/enhancer-binding protein alpha Human genes 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
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- 102000004190 Enzymes Human genes 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 108010028554 LDL Cholesterol Proteins 0.000 description 1
- 208000001145 Metabolic Syndrome Diseases 0.000 description 1
- 241000220214 Moringaceae Species 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 102000000536 PPAR gamma Human genes 0.000 description 1
- 108010016731 PPAR gamma Proteins 0.000 description 1
- 238000012356 Product development Methods 0.000 description 1
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 1
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 1
- 235000018742 Vitex negundo var cannabifolia Nutrition 0.000 description 1
- 244000271612 Vitex negundo var. cannabifolia Species 0.000 description 1
- 201000000690 abdominal obesity-metabolic syndrome Diseases 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 230000004129 fatty acid metabolism Effects 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 229940074391 gallic acid Drugs 0.000 description 1
- 235000004515 gallic acid Nutrition 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000001000 lipidemic effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- LGZXYFMMLRYXLK-UHFFFAOYSA-N mercury(2+);sulfide Chemical compound [S-2].[Hg+2] LGZXYFMMLRYXLK-UHFFFAOYSA-N 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 235000012149 noodles Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 229940049964 oleate Drugs 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000000291 postprandial effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 235000005875 quercetin Nutrition 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000020795 whole food diet Nutrition 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Medical Informatics (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses a kind of moringa oleifera leaf extractives with Lipid-lowering activities, its total phenolics and general flavone total content reach 50% or more, isoquercitrin is no less than 6%, astragalin is no less than 1.5%, Wei Caining 2 is no less than 1.5%, chlorogenic acid is no less than 2%, and isovitexin is no less than 0.15%, and Vitexin is no less than 0.15%.The invention also discloses the extracting methods of the moringa oleifera leaf extractive.The recovery rate that moringa oleifera leaf extractive is made in the present invention is higher, and component content is stablized.
Description
Technical field
The present invention relates to moringa oleifera leaf extractive more particularly to a kind of moringa oleifera leaf extractive with Lipid-lowering activities, the present invention
Further relate to the extracting method of the moringa oleifera leaf extractive.
Background technology
Moringa belongs to Moringaceae, Moringa, originates from the sub- Himalaya mountain zone of Indian province, is that one kind containing fully nutrient
Wholefood.Root, stem, leaf and the seed of Moringa contain abundant nutritional ingredient, the active constituent content highest in middle period.It is peppery
Wood is also rich in the polyphenol compounds such as a variety of medicinal ingredients, including phenolic acid, flavones and other trace elements.India and Africa
Country is usually used in treating the diseases such as diabetes, hypertension, cardiovascular disease, obesity.Moringa China Guangdong, Yunnan, Hainan and
There is introducing and planting in Sichuan etc..Moringa in 2012 is approved as new resource food, various Moringa product development fire by ministry of Health of China
Heat.So far, has the Moringas food such as leaf of Moringa noodles, horseradish tree chawing tablet, but the relevant health products of leaf of Moringa on the market
It does not appear on the market also with drug.One of reason for that is that, compared to for food, the standard of medicine of health products is more stringent, to
By leaf of Moringa research and development at health products and drug, content measuring standard need to be established, formulates the relevant health products standard of leaf of Moringa.
Invention content
One of the objects of the present invention is to provide a kind of moringa oleifera leaf extractives with Lipid-lowering activities.
Above-mentioned purpose of the present invention is achieved through the following technical solutions：A kind of moringa oleifera leaf extractive with Lipid-lowering activities,
Total phenolics and general flavone total content reach 50% or more, and isoquercitrin is no less than 6%, and astragalin is no less than 1.5%, and dimension is adopted
Rather -2 no less than 1.5%, chlorogenic acid is no less than 2%, and isovitexin is no less than 0.15%, and Vitexin is no less than 0.15%.
Moringa oleifera leaf extractive provided by the invention includes mainly total phenolics and general flavone, and the 6 kinds of active ingredients determined have
There is effect for reducing blood fat.Wherein, chlorogenic acid can effectively prevent fat and associated metabolic syndrome caused by diet, can pass through inhibition
The activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase adjusts cholesterol metabolic.Wei Caining -2 has collaboration reducing blood lipid and hypoglycemic
Effect.Vitexin -2 "-O- rhamnosides can significantly reduce low density fat sour (LDL-C) and the serum of triglycerides (TG) contains
Amount.Vitexin can reduce the expression of C/EBP α and PPAR γ in 3T3-L1 cells, to inhibit triglycerides in cell
Accumulation.Vitexin and isovitexin can reduce the postprandial blood sugar of mouse.Isoquercitrin can improve mouse liver fatty acid metabolism
It is disorderly.Therefore, moringa oleifera leaf extractive provided by the invention has preferable effect for reducing blood fat, can be used for preparing prevention fatty liver
Health food and drug.
It extracts acquisition by the following method：
(1) it crushes：Crush leaf of Moringa；
(2) it impregnates：Leaf of Moringa after smashing is impregnated using ethyl alcohol, obtains the mixture of leaf of Moringa and ethyl alcohol；
(3) it extracts：Heating and refluxing extraction is carried out to the mixture of the leaf of Moringa and ethyl alcohol, obtains leaf of Moringa crude extract；
(4) it purifies：The leaf of Moringa crude extract is purified through macroreticular resin HPD-100, obtains purification liquid；
(5) dry：The purifying Moringa leaf extract is concentrated under reduced pressure, is dry, obtains moringa oleifera leaf extractive.
In the step (2), a concentration of the 30~90% of ethyl alcohol, the solid-liquid ratio of leaf of Moringa and ethyl alcohol is 6~12:1；It impregnates
Time is 10-12h, preferably 12h.
Step (3) the heating and refluxing extraction temperature is 60~100 DEG C, extracts 2 times, each 3h, then merges 2 extractions
Liquid is concentrated to give leaf of Moringa crude extract.
In the step (4), add water by leaf of Moringa crude extract adjust leaf of Moringa concentration to 0.1g/mL and adjust pH be 3, make
For sample solution loading, sample solution volume is 2 times of macroreticular resin HPD-100 column volumes.
In the step (4), to be washed using ionized water, water volume is 10 times of macroreticular resin HPD-100 column volumes,
Then it is eluted using 70% ethyl alcohol as eluting solvent, eluting solvent dosage is 5 times of macroreticular resin HPD-100 column volumes.
The second object of the present invention is to provide a kind of extracting method of the moringa oleifera leaf extractive with Lipid-lowering activities, the party
Method crushes leaf of Moringa, and heating and refluxing extraction after solvent soaking is added, and gained leaf of Moringa crude extract is obtained by macroporous resin purification
To moringa oleifera leaf extractive, solve the problems, such as that moringa oleifera leaf extractive recovery rate is not high, and improve the stabilization of active constituent content
Property.
The two of the object of the invention are achieved through the following technical solutions：A kind of moringa oleifera leaf extractive with Lipid-lowering activities
Extracting method includes the following steps：
(1) it crushes：Crush leaf of Moringa；
(2) it impregnates：Leaf of Moringa after smashing is impregnated using ethyl alcohol, obtains the mixture of leaf of Moringa and ethyl alcohol；
(3) it extracts：Heating and refluxing extraction is carried out to the mixture of the leaf of Moringa and ethyl alcohol, obtains leaf of Moringa crude extract；
(4) it purifies：The leaf of Moringa crude extract obtains purification liquid by macroreticular resin HPD-100 purifying；
(5) dry：The purification liquid is concentrated under reduced pressure, is dry, obtains moringa oleifera leaf extractive.
In the step (2), a concentration of the 30~90% of ethyl alcohol, the solid-liquid ratio of leaf of Moringa and ethyl alcohol is 6~12:1, it impregnates
Time is 10-12h, preferably 12h.
Step (3) the heating and refluxing extraction temperature is 60~100 DEG C, extracts 2 times, each 3h, then merges 2 extractions
Liquid is concentrated to give leaf of Moringa crude extract.
In the step (4), add water that leaf of Moringa crude extract is adjusted leaf of Moringa concentration to 0.1g/mL, and it is 3 to adjust pH,
As sample solution loading, sample solution volume is 2 times of macroreticular resin HPD-100 column volumes.
In the step (4), to be washed using ionized water, water volume is 10 times of macroreticular resin HPD-100 column volumes,
Then it is eluted using 70% ethyl alcohol as eluting solvent, eluting solvent dosage is 5 times of macroreticular resin HPD-100 column volumes.
The present invention has the following advantages：
1. the Moringa extract recovery rate prepared by the extracting method provided is higher, total phenolics and general flavone total content reach
50% or more.
2. present invention optimization prepares the extraction and purification process of moringa oleifera leaf extractive.In order to more retain active ingredient, send out
A person of good sense has found HPD-100 resins to general flavone adsorbance and the equal highest of desorption quantity by screening to Multiple Type macroreticular resin,
Thus leaf of Moringa crude extract is purified using HPD-100, the Moringa extract component content obtained is high and stablizes,
In, total phenolics and general flavone total content reach 50% or more, and isoquercitrin is no less than 6%, and 1.5% is no less than containing astragalin,
It is no less than 1.5% containing Wei Caining -2,2% is no less than containing chlorogenic acid, is no less than 0.15% containing isovitexin, Vitexin is no less than
0.15%.Active ingredient type with effect for reducing blood fat is more, and content is high, thus has extraordinary lipid-lowering effect.
Description of the drawings
Fig. 1 is the oil red O stain figure of cellular control unit, model group cell and moringa oleifera leaf extractive group cell；
A control groups；B model groups：500 μm of olL of enuatrol-1；C moringa oleifera leaf extractive groups：200μg·mL-1。
Fig. 2 is the UPLC chromatograms for mixing contrast solution.
Fig. 3 is the UPLC chromatograms of test solution.
In Fig. 2 and Fig. 3：1. chlorogenic acid；2. Wei Caining -2；3. Vitexin；4. isovitexin；5. isoquercitrin；6. purple cloud
English glycosides.
Fig. 4 is to obtain the UPLC chromatograms of moringa oleifera leaf extractive using HPD-100 purifying resins.
Fig. 5 is to obtain the UPLC chromatograms of moringa oleifera leaf extractive using AB-8 purifying resins.
Specific implementation mode
Technical scheme of the present invention is further described by the following examples.
Embodiment 1
(1) it crushes：500g drying leaf of Moringa is weighed, leaf of Moringa is crushed to 16 mesh.
(2) it impregnates：Leaf of Moringa after crushing is impregnated using 70% ethyl alcohol of 4000mL, 12h is impregnated, obtains leaf of Moringa and second
The mixture of alcohol.
(3) it extracts：The mixture of leaf of Moringa and ethyl alcohol is fitted into the extractor that heating temperature is 90 DEG C and is heated back
Stream extraction 3h, filtering obtain first time extracting solution.The dregs of a decoction continue plus 70% ethyl alcohol heating and refluxing extraction 3h of 4000mL, and filtering obtains
Second of extracting solution merges No. 2 extracting solutions, is concentrated into 1L, obtains leaf of Moringa crude extract.
(4) it purifies：HPD-100 macroreticular resins fill column, column volume 500mL.Water is added to adjust leaf of Moringa leaf of Moringa crude extract
Concentration adjusts pH to 3 to 0.1g/mL, and loading, applied sample amount 1000ml are carried out as sample solution.Using 5000mL ionized waters into
It after row washing, is eluted with 70% ethyl alcohol of 2500mL, collects eluent, obtain purification liquid.
(5) dry：Freeze-drying is to get moringa oleifera leaf extractive after purification liquid is concentrated under reduced pressure.
Using sodium nitrite-aluminum nitrate-sodium-hydroxide method colour developing, measured using rutin as reference substance total in moringa oleifera leaf extractive
Flavones content；Using forint-phenol colorimetric method, total phenol acid content therein is measured by reference substance of gallic acid.Concrete operations are such as
Under：
Precision weighs moringa oleifera leaf extractive about 20mg, and test solution is made with 50% methanol.In addition it is accurately weighed take it is different
Quercitin, astragalin, Wei Caining -2, chlorogenic acid, Vitexin and isovitexin reference substance are appropriate, and mixing is made with 50% methanol
Reference substance solution, 22.2ug/mL containing isoquercitrin, astragalin 19.92ug/mL, 16.0ug/mL Wei Caining -2, chlorogenic acid
10.0ug/mL, Vitexin 2.42ug/mL, isovitexin 2.58ug/mL.Using phenomen Kinetex chromatographic columns (100 ×
4.6mm, 2.6 μm)；Mobile phase is -0.5% formic acid solution of acetonitrile, gradient elution：0~35min, acetonitrile:0.5% formic acid solution
=10.5%~23.5%:89.5%~76.5%；Detection wavelength is 334nm；Volume flow 0.35mL/min；30 DEG C of column temperature；
8 μ L of sample size.Mixed reference substance solution (Fig. 2) and test solution (Fig. 3) sample introduction are taken respectively, measure the content of 6 kinds of ingredients.
Gained moringa oleifera leaf extractive total phenolics and general flavone total content reach 50% or more, containing isoquercitrin 9.15%,
Containing astragalin 1.68%, the Wei Caining -2 contained is 2.02%, contains chlorogenic acid 2.03%, contains isovitexin 0.97%, Vitex negundo var cannabifolia
Element 0.62%.
Embodiment 2
(1) it crushes：500g drying leaf of Moringa is weighed, leaf of Moringa is crushed to 16 mesh.
(2) it impregnates：Leaf of Moringa after crushing is impregnated using 90% ethyl alcohol of 3000mL, 12h is impregnated, obtains leaf of Moringa and second
The mixture of alcohol.
(3) it extracts：The mixture of leaf of Moringa and ethyl alcohol is fitted into the extractor that heating temperature is 60 DEG C and is heated back
Stream extraction 3h, filtering obtain first time extracting solution.The dregs of a decoction continue plus 90% ethyl alcohol heating and refluxing extraction 3h of 3000mL, and filtering obtains
Second of extracting solution merges No. 2 extracting solutions, is concentrated into 1L, obtains leaf of Moringa crude extract.
(4) it purifies：HPD-100 macroreticular resins fill column, column volume 500mL.Water is added to adjust leaf of Moringa leaf of Moringa crude extract
Concentration adjusts pH to 3 to 0.1g/mL, and loading, applied sample amount 1000ml are carried out as sample solution.Using 5000mL ionized waters into
It after row washing, is eluted with 70% ethyl alcohol of 2500mL, collects eluent, obtain purification liquid.
(5) dry：Freeze-drying is to get moringa oleifera leaf extractive after purification liquid is concentrated under reduced pressure.
Gained moringa oleifera leaf extractive total phenolics are measured using 1 identical test method of embodiment and general flavone total content reaches
50% or more, containing isoquercitrin 6.76%, astragalin 3.61%, Wei Caining -2 is 2.44%, and chlorogenic acid 2.25% is different male
Jing Su 0.89%, Vitexin 0.59%.
Embodiment 3
(1) it crushes：500g drying leaf of Moringa is weighed, leaf of Moringa is crushed to 16 mesh.
(2) it impregnates：Leaf of Moringa after crushing is impregnated using 30% ethyl alcohol of 6000mL, 12h is impregnated, obtains leaf of Moringa and second
The mixture of alcohol.
(3) it extracts：The mixture of leaf of Moringa and ethyl alcohol is fitted into the extractor that heating temperature is 100 DEG C and is heated back
Stream extraction 3h, filtering obtain first time extracting solution.The dregs of a decoction continue plus 30% ethyl alcohol heating and refluxing extraction 3h of 6000mL, and filtering obtains
Second of extracting solution merges No. 2 extracting solutions, is concentrated into 1L, obtains leaf of Moringa crude extract.
(4) it purifies：HPD-100 macroreticular resins fill column, column volume 500mL.Water is added to adjust leaf of Moringa leaf of Moringa crude extract
Concentration adjusts pH to 3 to 0.1g/mL, and loading, applied sample amount 1000ml are carried out as sample solution.Using 5000mL ionized waters into
Row washing, is eluted with 70% ethyl alcohol of 2500mL, is collected eluent, is obtained purification liquid.
(5) dry：Freeze-drying is to get moringa oleifera leaf extractive after purification liquid is concentrated under reduced pressure.
Gained moringa oleifera leaf extractive total phenolics are measured using 1 identical test method of embodiment and general flavone total content reaches
50% or more, containing isoquercitrin 6.13%, astragalin 3.10%, Wei Caining -2 is 2.41%, and chlorogenic acid 2.01% is different male
Jing Su 0.20%, Vitexin 0.15%.
Embodiment 4
(1) it crushes：500g drying leaf of Moringa is weighed, leaf of Moringa is crushed to 16 mesh.
(2) it impregnates：Leaf of Moringa after crushing is impregnated using 50% ethyl alcohol of 5000mL, 12h is impregnated, obtains leaf of Moringa and second
The mixture of alcohol.
(3) it extracts：The mixture of leaf of Moringa and ethyl alcohol is fitted into the extractor that heating temperature is 80 DEG C and is heated back
Stream extraction 3h, filtering obtain first time extracting solution.The dregs of a decoction continue plus 50% ethyl alcohol heating and refluxing extraction 3h of 5000mL, and filtering obtains
Second of extracting solution merges No. 2 extracting solutions, is concentrated into 1L, obtains leaf of Moringa crude extract.
(4) it purifies：HPD-100 macroreticular resins are taken to fill column, column volume 500mL.Obtained leaf of Moringa crude extract is added into water tune
Whole leaf of Moringa concentration adjusts pH to 3 to 0.1g/mL, and loading, applied sample amount 1000ml are carried out as sample solution.Using 5000mL
It after ionized water is washed, is eluted with 70% ethyl alcohol of 2500mL, collects eluent, obtain purification liquid.
(5) dry：Freeze-drying is to get moringa oleifera leaf extractive after purification liquid is concentrated under reduced pressure.
Gained moringa oleifera leaf extractive is measured using 1 identical test method of embodiment and contains isoquercitrin 7.84%, purple cloud
English glycosides 2.78%, Wei Caining -2 are 1.50%, chlorogenic acid 3.42%, isovitexin 0.17%, Vitexin 0.15%.
Embodiment 5
(1) it crushes：500g drying leaf of Moringa is weighed, leaf of Moringa is crushed to 16 mesh.
(2) it impregnates：Leaf of Moringa after crushing is impregnated using 60% ethyl alcohol of 5000mL, 12h is impregnated, obtains leaf of Moringa and second
The mixture of alcohol.
(3) it extracts：The mixture of leaf of Moringa and ethyl alcohol is fitted into the extractor that heating temperature is 80 DEG C and is heated back
Stream extraction 3h, filtering obtain first time extracting solution.The dregs of a decoction continue plus 90% ethyl alcohol heating and refluxing extraction 3h of 5000mL, and filtering obtains
Second of extracting solution merges No. 2 extracting solutions, is concentrated into 1L, obtains leaf of Moringa crude extract.(4) it purifies：HPD-100 macroreticular resins
Fill column, column volume 500mL.Add water to adjust leaf of Moringa concentration to 0.1g/mL obtained leaf of Moringa crude extract, and adjust pH to 3,
Loading, applied sample amount 1000ml are carried out as sample solution.After being washed using 5000mL ionized waters, with 70% ethyl alcohol of 2500mL
It is eluted, collects eluent, obtain purification liquid.
(5) dry：Freeze-drying is to get moringa oleifera leaf extractive after purification liquid is concentrated under reduced pressure.
Gained moringa oleifera leaf extractive total phenolics are measured using 1 identical test method of embodiment and general flavone total content reaches
50% or more, containing isoquercitrin 9.52%, astragalin 1.56%, Wei Caining -2 is 1.96%, and chlorogenic acid 4.81% is different male
Jing Su 0.25%, Vitexin 0.16%.
Embodiment 6
(1) it crushes：500g drying leaf of Moringa is weighed, leaf of Moringa is crushed to 16 mesh.
(2) it impregnates：Leaf of Moringa after being crushed using the alcohol solution dipping of 6000mL 60%, is impregnated 12h, obtains Moringa
The mixture of leaf and ethyl alcohol.
(3) it extracts：The mixture of leaf of Moringa and ethyl alcohol is fitted into the extractor that heating temperature is 90 DEG C and is heated back
Stream extraction 3h, filtering obtain first time extracting solution.The dregs of a decoction continue plus 60% ethyl alcohol heating and refluxing extraction 3h of 3000mL, and filtering obtains
Second of extracting solution merges No. 2 extracting solutions, is concentrated into 1L, obtains leaf of Moringa crude extract.
(4) it purifies：HPD-100 macroreticular resins fill column, column volume 500mL.Water is added to adjust obtained leaf of Moringa crude extract
Leaf of Moringa concentration adjusts pH to 3 to 0.1g/mL, and loading, applied sample amount 1000ml are carried out as sample solution.Using 5000mL from
It after sub- water is washed, is eluted with 70% ethyl alcohol of 2500mL, collects eluent, obtain purification liquid.
(5) dry：Freeze-drying is to get moringa oleifera leaf extractive after purification liquid is concentrated under reduced pressure.
Gained moringa oleifera leaf extractive total phenolics are measured using 1 identical test method of embodiment and general flavone total content reaches
50% or more, containing isoquercitrin 8.20%, astragalin 3.67%, Wei Caining -2 is 1.88%, and chlorogenic acid 3.13% is different male
Jing Su 0.32%, Vitexin 0.21%.
Experimental example 1
(1) it crushes：500g drying leaf of Moringa is weighed, leaf of Moringa is crushed to 16 mesh.
(2) it impregnates：Leaf of Moringa after being crushed using the alcohol solution dipping of 6000mL 60%, is impregnated 12h, obtains Moringa
The mixture of leaf and ethyl alcohol.
(3) it extracts：The mixture of leaf of Moringa and ethyl alcohol is fitted into the extractor that heating temperature is 90 DEG C and is heated back
Stream extraction 3h, filtering obtain first time extracting solution.The dregs of a decoction continue plus 60% ethyl alcohol heating and refluxing extraction 3h of 3000mL, and filtering obtains
Second of extracting solution merges No. 2 extracting solutions, is concentrated into 1L, obtains leaf of Moringa crude extract.
(4) it purifies：Each model hole resin in table 1 is respectively adopted and fills column, column volume 500mL.Obtained leaf of Moringa is slightly carried
Liquid adds water to adjust leaf of Moringa concentration to 0.1g/mL, and adjusts pH to 3, and loading, applied sample amount 1000ml are carried out as sample solution.It adopts
It after being washed with 5000mL ionized waters, is eluted with 70% ethyl alcohol of 2500mL, collects eluent, obtain purification liquid.
Using sodium nitrite-aluminum nitrate-sodium-hydroxide method colour developing, measured using rutin as reference substance total in moringa oleifera leaf extractive
Flavones content.As it is in table 1 the results show that HPD-100 macroreticular resins to the adsorbance of general flavone, than elution amount, adsorption rate and
Desorption efficiency is above AB-8 macroreticular resins.As it can be seen that being extracted to leaf of Moringa using HPD-100 macroreticular resins, the extraction of acquisition
Object general flavone content higher.
The Moringa leaf extract of HPD-100 macroreticular resins and AB-8 macroporous resin purifications is taken to carry out UPLC analyses, tool respectively
Body method is referring to embodiment 1.By being shown in Table result shown in 2, Fig. 4-5 as it can be seen that the leaf of Moringa of HPD-100 macroporous resin purifications extracts
Wei Caining -2 contents are 2.44% in liquid, and 62.7% or so is improved than AB-8 macroporous resin purification.
The macroporous absorbent resin Dynamic Adsorption the selection result of 1 two kinds of different models of table
Table 2 purifies to obtain the percentage composition of Wei Caining -2 in moringa oleifera leaf extractive using two kinds of different resins
| Resin model | Retention time/min | Percentage composition/% |
| HPD-100 | 6.994 | 2.44 |
| AB-8 | 6.845 | 1.50 |
Experimental example 2：The external Lipid-lowering activities of moringa oleifera leaf extractive are tested
Using 500 μm of olL-1Sodium oleate solution establishes HepG2 cellular fat Accumulation Model groups.Take appropriate embodiment 1 peppery
The wooden leaf extract adds culture solution to be configured to 200 μ g/mL solution, and HepG2 cell culture is added, establishes moringa oleifera leaf extractive group.It takes
Culture solution culture HepG2 cells, establish control group.It takes Effects of Xuezhikang that culture solution is added to be configured to 150 μ g/mL solution, establishes Effects of Xuezhikang
Group.It takes Simvastatin that culture solution is added to be configured to 100 μ g/mL solution, establishes Simvastatin group.Culture solution is by DMEM cell culture
Liquid, RPMI-1640 cell culture fluids, fetal calf serum and PBS solution configure.Wherein, DMEM cell culture fluids (article number：
C11995500BT), RPMI-1640 cell culture fluids (article number：C11875500BT), fetal calf serum (article number：10270-
106) it is U.S.'s Gibco Products；PBS solution (article number：SH30256.01) it is Hyclone Products.
Compared by oil red O stain observation, the results are shown in Figure 1, and the Chinese red fat drips of moringa oleifera leaf extractive group compare mould
The color of type group wants shallow quantity less, illustrates the intracellular triglycerides of moringa oleifera leaf extractive group (TG) cumulant compared with model group
It is low.
After each group cell culture for 24 hours, cell suspending liquid is prepared, lytic cell measures each group with triglycerides enzyme reagent kit
Intracellular TG contents, as a result such as table 1.The TG contents of moringa oleifera leaf extractive group, the Effects of Xuezhikang group of positive control and Simvastatin group
There is decline compared with model group, with significant difference compared with model group.The HepG2 cellular fats of fatty acid-induced are accumulated
There is prodigious similitude, experimental result to show that moringa oleifera leaf extractive can be reduced effectively carefully for model and the pathological characteristics of fatty liver
The content of triglyceride of intracellular has certain preventive and therapeutic effect to fatty liver.
1 moringa oleifera leaf extractive of table TG intracellular to HepG2 influence (N=6)
Compared with the control group,###p<0.001；Compared with model group, * * p<0.05
The moringa oleifera leaf extractive of Example 2-6 carries out above-mentioned experiment, shows that moringa oleifera leaf extractive can be reduced effectively carefully
The content of triglyceride of intracellular has certain preventive and therapeutic effect to fatty liver.
The present invention can be summarized with others without prejudice to the concrete form of the spirit or essential characteristics of the present invention.The present invention's
The embodiment above can only all be considered the description of the invention rather than limit, therefore every substantial technological according to the present invention
To any subtle modifications, equivalent variations and modifications made by above example, belong in the range of technical solution of the present invention.
Claims (9)
1. a kind of moringa oleifera leaf extractive with Lipid-lowering activities, which is characterized in that total phenolics and general flavone total content reach 50%
More than, isoquercitrin is no less than 6%, and astragalin is no less than 1.5%, and Wei Caining -2 is no less than 1.5%, and chlorogenic acid is no less than
2%, isovitexin is no less than 0.15%, and Vitexin is no less than 0.15%.
2. a kind of moringa oleifera leaf extractive with Lipid-lowering activities according to claim 1, which is characterized in that it passes through following
Method extraction obtains：
(1) it crushes：Crush leaf of Moringa；
(2) it impregnates：Leaf of Moringa after smashing is impregnated using ethyl alcohol, obtains the mixture of leaf of Moringa and ethyl alcohol；
(3) it extracts：Heating and refluxing extraction is carried out to the mixture of the leaf of Moringa and ethyl alcohol, obtains leaf of Moringa crude extract；
(4) it purifies：The leaf of Moringa crude extract obtains purification liquid by macroreticular resin HPD-100 purifying；
(5) dry：The purification liquid is concentrated under reduced pressure, is dry, obtains moringa oleifera leaf extractive.
3. a kind of moringa oleifera leaf extractive with Lipid-lowering activities according to claim 1, which is characterized in that the step
(2) in, concentration of alcohol is 30~90%, and the solid-liquid ratio of leaf of Moringa and ethyl alcohol is 6~12:1, soaking time 10-12h.
4. a kind of moringa oleifera leaf extractive with Lipid-lowering activities according to claim 1, which is characterized in that the step
(3) heating and refluxing extraction temperature is 60~100 DEG C, extracts 2 times, each 3h, then merges No. 2 extracting solutions, be concentrated to give Moringa
Leaf crude extract.
5. a kind of moringa oleifera leaf extractive with Lipid-lowering activities according to claim 1, which is characterized in that the step
(4) in, add water that leaf of Moringa crude extract is adjusted leaf of Moringa concentration to 0.1g/mL, and it is 3 to adjust pH, as sample solution loading, on
Sample liquid volume is 2 times of macroreticular resin HPD-100 column volumes；It is washed using ionized water, water volume is macroreticular resin HPD-
Then 10 times of 100 column volumes are eluted using 70% ethyl alcohol as eluting solvent, eluting solvent dosage is macroreticular resin HPD-
5 times of 100 column volumes.
6. a kind of extracting method of the moringa oleifera leaf extractive with Lipid-lowering activities, which is characterized in that include the following steps：
(1) it crushes：Crush leaf of Moringa；
(2) it impregnates：Leaf of Moringa after smashing is impregnated using ethyl alcohol, obtains the mixture of leaf of Moringa and ethyl alcohol；
(3) it extracts：Heating and refluxing extraction is carried out to the mixture of the leaf of Moringa and ethyl alcohol, obtains leaf of Moringa crude extract；
(4) it purifies：The leaf of Moringa crude extract obtains purification liquid by macroreticular resin HPD-100 purifying；
(5) dry：The purification liquid is concentrated under reduced pressure, is dry, obtains moringa oleifera leaf extractive.
7. a kind of moringa oleifera leaf extractive with Lipid-lowering activities according to claim 6, which is characterized in that the step
(2) in, concentration of alcohol is 30~90%, and the solid-liquid ratio of leaf of Moringa and ethyl alcohol is 6~12:1, soaking time 10-12h.
8. a kind of moringa oleifera leaf extractive with Lipid-lowering activities according to claim 6, which is characterized in that the step
(3) heating and refluxing extraction temperature is 60~100 DEG C, extracts 2 times, each 3h, then merges No. 2 extracting solutions, be concentrated to give Moringa
Leaf crude extract.
9. a kind of moringa oleifera leaf extractive with Lipid-lowering activities according to claim 6, which is characterized in that the step
(4) in, add water that leaf of Moringa crude extract is adjusted leaf of Moringa concentration to 0.1g/mL, and it is 3 to adjust pH, as sample solution loading, on
Sample liquid volume is 2 times of macroreticular resin HPD-100 column volumes；It is washed using ionized water, water volume is macroreticular resin HPD-
Then 10 times of 100 column volumes are eluted using 70% ethyl alcohol as eluting solvent, eluting solvent dosage is macroreticular resin HPD-
5 times of 100 column volumes.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109602769A (en) * | 2018-12-29 | 2019-04-12 | 南昌大学第附属医院 | Moringa oleifera leaf extractive and its preparation method and application |
| CN109806285A (en) * | 2019-03-13 | 2019-05-28 | 华南理工大学 | One kind having active moringa oleifera leaf extractive of anti-trioxypurine and the preparation method and application thereof |
| CN110037305A (en) * | 2019-05-28 | 2019-07-23 | 云南熊牌生物科技有限公司 | A kind of preparation method of moringa oleifera leaf extractive |
| CN110596263A (en) * | 2019-08-23 | 2019-12-20 | 广州泽力医药科技有限公司 | Establishing method of moringa oleifera extract fingerprint and fingerprint thereof |
| CN114209721A (en) * | 2021-11-12 | 2022-03-22 | 华南理工大学 | Moringa oleifera leaf polyphenol-polysaccharide composition capable of reducing blood sugar and controlling lipid as well as preparation method and application of moringa oleifera leaf polyphenol-polysaccharide composition |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109602769A (en) * | 2018-12-29 | 2019-04-12 | 南昌大学第附属医院 | Moringa oleifera leaf extractive and its preparation method and application |
| CN109806285A (en) * | 2019-03-13 | 2019-05-28 | 华南理工大学 | One kind having active moringa oleifera leaf extractive of anti-trioxypurine and the preparation method and application thereof |
| CN109806285B (en) * | 2019-03-13 | 2021-08-10 | 华南理工大学 | Moringa oleifera leaf extract with uric acid reducing activity and preparation method and application thereof |
| CN110037305A (en) * | 2019-05-28 | 2019-07-23 | 云南熊牌生物科技有限公司 | A kind of preparation method of moringa oleifera leaf extractive |
| CN110596263A (en) * | 2019-08-23 | 2019-12-20 | 广州泽力医药科技有限公司 | Establishing method of moringa oleifera extract fingerprint and fingerprint thereof |
| CN114209721A (en) * | 2021-11-12 | 2022-03-22 | 华南理工大学 | Moringa oleifera leaf polyphenol-polysaccharide composition capable of reducing blood sugar and controlling lipid as well as preparation method and application of moringa oleifera leaf polyphenol-polysaccharide composition |
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