CN103169788B - Chinese date leaf extractive and application thereof in preparation of medicament or health food for preventing and treating liver injury - Google Patents
Chinese date leaf extractive and application thereof in preparation of medicament or health food for preventing and treating liver injury Download PDFInfo
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Abstract
The invention discloses a Chinese date leaf extractive and an application thereof in preparation of a medicament or health food for preventing and treating liver injury. The way that macroporous resin is coupled with polyamide resin and then a fine Chinese date leaf extractive is prepared through a preparative liquid phase is firstly adopted; compared with the traditional separation and purification method, the way has the advantages that the separation efficiency is higher, the active component obtained through separation is higher in content, strong in biological activity and high in safety. Experiment researches show that the Chinese date leaf extractive can be used for remarkably reducing the activities of ALT (Alanine Transaminase) and AST (Aspartate Transaminase) in serum of a model mouse with liver injury caused by CCl4, D-GalN and ethanol and the content of MDA (Methyl Di Aldehyde) in liver tissues and enhancing the activity of SOD (Superoxide Dismutase) of the liver and has favorable preventing and treating effects. Therefore, Chinese date leaves and the Chinese date leaf extractive are expected to be developed into a new-generation safe and effective medicament or health food for preventing and treating liver injury, and meanwhile, an approach is also provided for turning the waste Chinese date leaf resource into the things of value and comprehensively utilizing a date tree resource.
Description
Technical field
The present invention relates to extractive technique and the application thereof of a Plant Extracts, be specifically related to the extractive technique of Folium Jujubae extract and the application in preparation control liver injury medicament or health product thereof.
Background technology
Liver is one of most important internal organs of body, and hepatic disease is comparatively common clinically disease.Therefore, hepatic disease is one of object of medical science primary study always.Hepatic injury is the total pathological state a kind of with conspicuous characteristics of multiple hepatic disease, and human health in serious threat.Various harmful factors such as medicine, virus, ethanol, biology etc. may cause liver function to have infringement in various degree, thereby make removing toxic substances, the excretory function of liver and stock with regeneration capacity and reduce, hepatic blood flow reduces, metabolic burden increases the weight of, thereby environment disturbance in occurring, hepatic necrosis and apoptosis, and then cause hepatic injury.The whole world has billions of people once or just standing the misery of hepatic injury.The long-term existence of hepatic injury tends to cause hepatic fibrosis, is and then brings out liver cirrhosis, liver failure, the important initiating agent of hepatocarcinoma even.Therefore preventing and treating hepatocyte injury is one of important step of liver disease clinically, is the basis that suppresses the Occurrence and development of diseases such as hepatic fibrosis, hepatic necrosis, liver cirrhosis and hepatocarcinoma.Therefore study hepatic injury control medicine and there is important clinical significance.Clinical conventional hepatic at present, or because expensive, or because using inconvenience, or thering is larger side effect, use is restricted.Therefore, further find the striving direction that remains current pharmacology worker for the active drug preventing and treat hepatic injury.
The dried leaves of Fructus Jujubae leaf system Rhamnaceae jujube Fructus Jujubae (Ziziphus jujuba Mill.).Book on Chinese herbal medicine Folium Jujubae can be used for treating the diseases such as fever of children, furuncle, Re Rush, rotten foot, burn and scald as medicinal.Modern study about Folium Jujubae is less, and except on a small quantity, as the application of tea-drinking development of raw materials, how less being utilized, abandon as waste resource.Have not yet to see the technology of preparing activity extract taking Folium Jujubae as raw material, also have no and be applied to the control medicine of hepatic injury or the report of health food taking Folium Jujubae as chief component component.
Summary of the invention
Goal of the invention: the object of the invention is in order to overcome the deficiencies in the prior art, on the basis of the original purposes of Folium Jujubae, develop its new clinical application, the application of Folium Jujubae in control hepatic injury is provided, experimental result shows, it has the effect of good control hepatic injury.Another object of the present invention is openly a kind of preparation method of Folium Jujubae extract.
Technical scheme: in order to realize above object, the technical scheme that the present invention takes is:
A kind of Folium Jujubae extract, it is to prepare by following steps:
(1) get after Folium Jujubae is chosen, cleans, is dried and put into extraction pot, add 50%～95% ethanol of 6～12 times of amounts, reflux 1～3 time, filtration, merge extractive liquid,, be concentrated into without after alcohol taste, and the ethanol extract that obtains Folium Jujubae is for subsequent use;
(2) get the Folium Jujubae ethanol extract that step (1) obtains, add 1～5 times of water gaging, upper macroporous resin column, first water and concentration are 10% ethanol elution respectively, the ethanol elution that is 60%～95% by concentration again, collect 60%～95% ethanol elution, concentrated, drying under reduced pressure obtains Folium Jujubae active site crude extract, for subsequent use;
(3) get polyamide column in the Folium Jujubae active site crude extract dry method that step (2) obtains, first distinguish water and 20% ethanol elution, be 70%～95% ethanol elution by concentration again, and collect 70%～95% ethanol elution, concentrated, obtain the refining extract of Folium Jujubae active site through drying under reduced pressure;
(4) get step (3) and obtain Folium Jujubae active site extract and adopt preparation liquid phase further enrichment to obtain refining Fructus Jujubae extract, after testing, total triterpene and general flavone content >80%.
As preferred version, above-described Folium Jujubae extract, in step (1), extraction conditions is to add the ethanol that 10 times of amount concentration are 80%, is heated to 80 DEG C and continues to reflux 2 hours.The method can ensure to extract and between solution and medical material, reach enough large concentration difference, increase the dissolution rate of target component, because concentration of alcohol is too low, triterpenic acid active component yield in Folium Jujubae is too low, the flavonoid glycoside composition rate of transform in the too high Folium Jujubae of concentration of alcohol is too low, and the impurity rate of transform such as fatty acid, chlorophyll increases.Therefore the present invention, according to the character of active component, determines concentration and the consumption of ethanol, and the extract target component dissolution rate obtaining is high, and activity is stronger.
As preferred version, above-described Folium Jujubae extract, the macroporous adsorbent resin described in step (2) is nonpolar macroporous adsorption resin.As more preferably scheme, wherein the nonpolar macroporous adsorption resin described in step (2) is D101 type macroporous adsorbent resin, and collecting concentration is 90% ethanol elution; In step (3), collecting concentration is 90% ethanol elution.The method that the present invention combines with modern times extraction separation means and Pharmacological Activity Screening is determined the best control of Folium Jujubae hepatic injury activity extract, show through lot of experiments, by nonpolar macroporous adsorbing coupled polyamide column chromatography, adopt the ethanol elution of variable concentrations, be conducive to flavonoid in Folium Jujubae and the enrichment of triterpenes active component, remove saccharide, tannin, the impurity such as chlorophyll, obtain high activity component ratio and active stronger control hepatic injury activity extract, detect triterpene acids and flavones ingredient portion content in this active site through HPLC-ELSD and HPLC-DAD and can reach 70%.
As preferred version, above-described Folium Jujubae extract, the particle diameter of the polyamide described in step (3) is 60-80 order.
As preferred version, above-described Folium Jujubae extract, the chromatographic condition of the preparation liquid phase enrichment described in step (4) is: chromatographic column is the anti-phase C18 post of Agilent, mobile phase is methanol-0.5% phosphate aqueous solution gradient elution, and the condition of gradient elution is: 0 to 35 minute, the content of methanol rose to 45% by 5%, the content of 35 to 60 minutes methanol rises to 83% by 45%, detection wavelength is 285nm, and flow velocity is 5ml/min, and detector is diode display detector.
The application in preparation control liver injury medicament or health product of Folium Jujubae and extract thereof.
The present invention shows through lot of experiments; Folium Jujubae and extract thereof can effectively reduce liver injury model animal serum ALT, AST activity and Liver MDA; strengthen liver SOD activity; show that Folium Jujubae has obvious liver protection, can be applicable to medicine or the health food of preparation control hepatic injury.
The application of Folium Jujubae extract of the present invention in medicine or the health food of preparation control hepatic injury, makes Fructus Jujubae extract and pharmaceutically acceptable carrier the medicine of tablet, injection, capsule, granule, pill, micropill, powder, drop pill, decoction, syrup, mixture, soft extract or extractum dosage form.
When Folium Jujubae extract provided by the invention is made capsule Folium Jujubae extract and carrier lactose or corn starch mix homogeneously, granulate, the then encapsulated capsule of making.
When Folium Jujubae extract provided by the invention is made to tablet, Folium Jujubae extract and lactose or corn starch, while needs, add magnesium stearate lubricant, mix homogeneously, then tabletting is made tablet.
When Folium Jujubae extract granulation agent provided by the invention, Folium Jujubae extract and diluent lactose or corn starch mix homogeneously, granulate, dry, granulation agent.
When Folium Jujubae extract provided by the invention is made injection, getting Folium Jujubae extract adds physiological saline solution then to add activated carbon, stir, 80 DEG C are heated 30 minutes, filter, regulate pH value, be filtered to clear and bright with sintered glass funnel or other filter, fill, makes injection for 30 minutes 100 to 115 DEG C of sterilizings.
When Folium Jujubae extract provided by the invention is made other dosage form, can prepare by pharmacy conventional method.
Beneficial effect: the present invention carries out new purposes exploitation on the basis of the original application of Folium Jujubae, prepares its activity extract simultaneously, compares and has the following advantages with existing prior art:
(1) the present invention, mainly to produce the garbage Folium Jujubae producing in Fructus Jujubae process as raw material, prepares its activity extract, develops its new purposes, realization is turned waste into wealth, what promoted jujube tree can value, has realized the comprehensive utilization of jujube resources, has good economic benefits.
(2) Folium Jujubae control hepatic injury activity extract provided by the invention, experimental result shows significantly to reduce CCl
4cause that liver injury model mice, ethanol cause liver injury model mice, D-Gal causes liver injury model mice serum ALT, AST activity and Liver MDA; strengthen liver SOD activity; show that this activity extract is not only to chemical liver injury, drink and cause hepatic injury and have obvious liver protection for a long time, hepatic injury due to viral hepatitis is also had to protective effect simultaneously.Therefore Folium Jujubae and extract thereof are expected to exploitation to become a new generation safe and effective, for preventing and treating medicine or the health product of polytype hepatic injury, simultaneously also for Folium Jujubae waste resource is turned waste into wealth and jujube resources comprehensive utilization provides approach.
(3) preparation method provided by the invention is workable, especially in separation process, the present invention adopts macroporous resin and polyamide coupling first, and the further enriching and purifying of employing preparation liquid phase, extraction separation efficiency is high, separates the Folium Jujubae extract active component content obtaining high, and antihepatitis activity is stronger, untoward reaction is lower, and consuming little energy in preparation technology, production cost is low, environmentally safe.
Detailed description of the invention
Further illustrate the present invention below in conjunction with specific embodiment, should understand these embodiment is only not used in and limits the scope of the invention for the present invention is described, after having read the present invention, those skilled in the art all fall within the application's claims limited range to the amendment of the various equivalent form of values of the present invention.
Embodiment 1
Folium Jujubae control hepatic injury activity extract, it prepares by the following method:
(1) get after Folium Jujubae is chosen, cleans, is dried and take 10kg, put into extraction pot, add 80% ethanol of 10 times of amounts, reflux 3 times, each 2h, filtration, merge extractive liquid,, be concentrated into without after alcohol taste, and the ethanol extract (2L) that obtains Folium Jujubae is for subsequent use;
(2) get the Folium Jujubae ethanol extract that step (1) obtains, add 2 times of water gagings, after mixing, adding activated diameter is 50 centimetres, and in the D101 macroporous adsorption resin chromatography post of high 1.5 meters, preadsorption is after 12 hours, press respectively the speed eluting of 2BV/h with water, 10% ethanol and 90% ethanol, elution volume is 50L, collects 90% ethanol elution, is concentrated into without alcohol taste, obtain Folium Jujubae active site crude extract 1L, for subsequent use;
(3) getting activated diameter in the Folium Jujubae active site crude extract dry method that step (2) obtains is 20 centimetres, the polyamide column (60～80 order) of high 1.5 meters, first use respectively water and 20% ethanol elution of 20L, be 90% ethanol elution by concentration again, and collect 90% ethanol elution, concentrated, obtain the refining extract 610g of Folium Jujubae active site through drying under reduced pressure;
(4) get step (3) and obtain Folium Jujubae active site extract and adopt preparation liquid phase further enrichment to obtain refining Fructus Jujubae extract, after testing, total triterpene and general flavone content >80%.Preparative liquid chromatography condition is: chromatographic column is the anti-phase C18 post of Agilent, mobile phase is methanol-0.5% phosphate aqueous solution gradient elution, the condition of gradient elution is: 0 to 35 minute, the content of methanol rises to 45% by 5%, the content of 35 to 60 minutes methanol rises to 83% by 45%, detection wavelength is 285nm, and flow velocity is 5ml/min, and detector is diode display detector.
Embodiment 2
Folium Jujubae control hepatic injury activity extract, it prepares by the following method:
(1) get after Folium Jujubae is chosen, cleans, is dried and take 10kg, put into extraction pot, add 70% ethanol of 10 times of amounts, reflux 3 times, each 2h, filtration, merge extractive liquid,, be concentrated into without after alcohol taste, and the ethanol extract (2.5L) that obtains Folium Jujubae is for subsequent use;
(2) get the Folium Jujubae ethanol extract that step (1) obtains, add 1.5 times of water gagings, after mixing, adding activated diameter is 50 centimetres, and in the AB-8 macroporous adsorption resin chromatography post of high 1.5 meters, preadsorption is after 12 hours, press respectively the speed eluting of 2BV/h with water, 10% ethanol and 85% ethanol, elution volume is 50L, collects 85% ethanol elution, is concentrated into without alcohol taste, obtain Folium Jujubae active site crude extract 1L, for subsequent use;
(3) getting activated diameter in the Folium Jujubae active site crude extract dry method that step (2) obtains is 20 centimetres, the polyamide column (60～80 order) of high 1.5 meters, first use respectively water and 20% ethanol elution of 20L, be 80% ethanol elution by concentration again, and collect 80% ethanol elution, concentrated, obtain the refining extract 570g of Folium Jujubae active site through drying under reduced pressure;
(4) get step (3) and obtain Folium Jujubae active site extract and adopt preparation liquid phase further enrichment to obtain refining Fructus Jujubae extract, after testing, total triterpene and general flavone content >80%.Preparative liquid chromatography condition is: chromatographic column is the anti-phase C18 post of Agilent, mobile phase is methanol-0.5% phosphate aqueous solution gradient elution, the condition of gradient elution is: 0 to 35 minute, the content of methanol rises to 45% by 5%, the content of 35 to 60 minutes methanol rises to 83% by 45%, detection wavelength is 285nm, and flow velocity is 5ml/min, and detector is diode display detector.
Embodiment 3
Folium Jujubae control hepatic injury activity extract, it prepares by the following method:
(1) get after Folium Jujubae is chosen, cleans, is dried and take 10kg, put into extraction pot, add 90% ethanol of 8 times of amounts, reflux 2 times, each 2h, filtration, merge extractive liquid,, be concentrated into without after alcohol taste, and the ethanol extract (1.6L) that obtains Folium Jujubae is for subsequent use;
(2) get the Folium Jujubae ethanol extract that step (1) obtains, add 3 times of water gagings, after mixing, adding activated diameter is 50 centimetres, and in the HP-20 macroporous adsorption resin chromatography post of high 1.5 meters, preadsorption is after 12 hours, press respectively the speed eluting of 2BV/h with water, 10% ethanol and 95% ethanol, elution volume is 50L, collects 95% ethanol elution, is concentrated into without alcohol taste, obtain Folium Jujubae active site crude extract 1L, for subsequent use;
(3) getting activated diameter in the Folium Jujubae active site crude extract dry method that step (2) obtains is 20 centimetres, the polyamide column (100 order) of high 1.5 meters, first use respectively water and 20% ethanol elution of 20L, be 95% ethanol elution by concentration again, and collect 80% ethanol elution, concentrated, obtain the refining extract 6050g of Folium Jujubae active site through drying under reduced pressure;
(4) get step (3) and obtain Folium Jujubae active site extract and adopt preparation liquid phase further enrichment to obtain refining Fructus Jujubae extract, after testing, total triterpene and general flavone content >81%.Preparative liquid chromatography condition is: chromatographic column is the anti-phase C18 post of Agilent, mobile phase is methanol-0.5% phosphate aqueous solution gradient elution, the condition of gradient elution is: 0 to 35 minute, the content of methanol rises to 45% by 5%, the content of 35 to 60 minutes methanol rises to 83% by 45%, detection wavelength is 285nm, and flow velocity is 5ml/min, and detector is diode display detector.
Embodiment 4
The experimentation of Folium Jujubae extract anti-liver injury
One, experiment material and medicine
1. medicine and reagent
AST, ALT, MDA, SOD test kit and Coomassie brilliant blue protein reagent box are all purchased from Nanjing and build up Bioengineering Research Institute; Carbon tetrachloride (CCl
4, analytical pure, Shanghai Ling Feng chemical reagent company limited), when use, be mixed with 0.1% peanut oil solution with Oleum Arachidis hypogaeae semen; Galactosaminide (D-GalN, Sigma company), distilled water dissolved dilution becomes 70mg/mL solution before use; Bifendate Tablet (Jiangsu Pengyao Pharmaceutical Co., Ltd.), faces the used time to be ground into fine powder and to add normal saline and make suspension.
2. laboratory animal
Clean level male ICR mouse, body weight 18～22g, is provided by Zhejiang Province's Experimental Animal Center, quality certification SCXK(Zhejiang) 2008-0033.
3. experimental apparatus
Enzyme linked immunological instrument (Bio-Tek company of the U.S.); UV-2000 type ultraviolet spectrophotometer (Beijing Lai Baitaike Instrument Ltd.); BT125 type electronic balance (Sai Duolisi scientific instrument company limited); KQ-250E type ultrasonic cleaner (Kunshan He Chuan ultrasonic instrument company limited); AnkeGL-16GII type centrifuge (Anting Scientific Instrument Factory, Shanghai).
4. tested medicine and processing method
Get respectively the Folium Jujubae extract that embodiment 1～3 prepares, thin up is made desired concn; Positive drug is bifendate, before administration, dissolves and is mixed with desired concn with distilled water.
Two, experimental technique
1. couple CCl
4cause the impact of acute liver
Get 60 mices, adaptability was fed after 1 week, was divided at random 6 groups by body weight, 10 every group, was respectively Normal group, CCl
4to liver injury model group, Folium Jujubae extract low dose group (0.25g/kg/d), middle dosage group (0.5g/kg/d) and high dose group (1.0g/kg/d) that embodiment 2 prepares, bifendate group (150mg/kg/d).Matched group and model group give commensurability normal saline, the continuous gavage 10d of administration group, and 1h after last administration, except normal group ip0.9%NaCl10mL/kg, all the other respectively organize equal ip0.1%CCl
4peanut oil solution 10mL/kg.After 16h, all mices adopt the blood sampling of eye socket rear vein beard, separation of serum, the activity of mensuration serum alt and AST.After getting blood, put to death mice, get immediately part liver, be prepared into 10% liver homogenate with normal saline, measure MDA content and SOD vigor according to test kit explanation.
2. D-GalN is caused to the impact of acute liver
Get 60 mices, adaptability was fed after 1 week, be divided at random 6 groups by body weight, every group 10, be respectively Normal group, D-GalN causes liver injury model group, Folium Jujubae extract low dose group (0.25g/kg/d), middle dosage group (0.5g/kg/d) and high dose group (1.0g/kg/d) that embodiment 1 prepares, bifendate group (150mg/kg/d).Matched group and model group give commensurability normal saline, the continuous gavage 10d of administration group, and 1h after last administration, except normal group ip0.9%NaCl10mL/kg, all the other respectively organize equal ipD-GalN700mg/kg.After 16h, all mices adopt the blood sampling of eye socket rear vein beard, separation of serum, the activity of mensuration serum alt and AST.After getting blood, put to death mice, get immediately part liver, be prepared into 10% liver homogenate with normal saline, measure MDA content and SOD vigor according to test kit explanation.
3. ethanol is caused to the impact of mouse liver injury
Get 60 mices, adaptability was fed after 1 week, be divided at random 6 groups by body weight, every group 10, be respectively Normal group, ethanol causes liver injury model group, Folium Jujubae extract low dose group (0.25g/kg/d), middle dosage group (0.5g/kg/d) and high dose group (1.0g/kg/d) that embodiment 3 prepares, bifendate group (150mg/kg/d).Except Normal group (with isopyknic distilled water gavage), all the other each groups all give 50% ethanol by 7mL/kg/ gavage, and 2 times/d, midfeather 6h, continuously 15d.From modeling the 1st day, every day gastric infusion 1 time.Normal group and model control group gavage isopyknic distilled water, and all the other are respectively organized gavage respectively and give corresponding Folium Jujubae extract and bifendate.After last 1 gavage 6h, all mices adopt the blood sampling of eye socket rear vein beard, separation of serum, the activity of mensuration serum alt and AST.After getting blood, put to death mice, get immediately part liver, be prepared into 10% liver homogenate with normal saline, measure MDA content and SOD vigor according to test kit explanation.
4. statistical procedures
All experimental datas all adopt SPSS11.5 statistical processing software to carry out statistical procedures, result with
represent, between group, relatively adopt variance analysis.
Three, experimental result
1. Folium Jujubae extract is to CCl
4cause the impact of acute liver
CCl
4liver injury model group and normal group comparison, mice serum ALT, AST and hepatic tissue MDA level obviously raise (P<0.01), active obviously reduce (P<0.01) of SOD; The each dosage group of Folium Jujubae extract, bifendate group and the model group that Folium Jujubae, embodiment 2 prepare makes hepatic injury mice serum ALT, AST reduce (P<0.01) more to some extent; Folium Jujubae extract low dose group has the hepatic tissue of reduction MDA level, the trend of rising hepatic tissue SOD level, but there was no significant difference (P>0.05); Folium Jujubae extracts object height, middle dosage group and bifendate group and model group makes hepatic injury murine liver tissue MDA level reduce (P<0.05) more to some extent, SOD level rising (P<0.05).Specific experiment the results are shown in Table 1.
Table 1 Folium Jujubae extract is to CCl
4cause the impact (mean ± SD, n=10) of acute hepatic injury mice Serum ALT, AST and liver SOD, MDA
Note: with model group comparison,
##p<0.01,
#p<0.05.
2. Folium Jujubae extract causes the impact of acute liver on D-GalN
D-GalN liver injury model group and normal group comparison, mice serum ALT, AST and hepatic tissue MDA level obviously raise (P<0.01), active obviously reduce (P<0.01) of SOD; Folium Jujubae, the each dosage group of Folium Jujubae extract, bifendate group and the model group that embodiment 1 prepares makes hepatic injury mice serum ALT, AST reduce (P<0.01) more to some extent; The each dosage group of Folium Jujubae extract and bifendate group and model group make hepatic injury murine liver tissue MDA level reduce (P<0.05) more to some extent, SOD level rising (P<0.05).Specific experiment the results are shown in Table 2.
Table 2 Folium Jujubae extract causes the impact (mean ± SD, n=10) of acute hepatic injury mice Serum ALT, AST and liver SOD, MDA on D-GalN
Note: with model group comparison,
##p<0.01,
#p<0.05.
3. Folium Jujubae extract causes the impact of acute liver on ethanol
Ethanol causes liver injury model group and normal group comparison, and mice serum ALT, AST and hepatic tissue MDA level obviously raise (P<0.01), active obviously reduce (P<0.01) of SOD; Folium Jujubae, the each dosage group of Folium Jujubae extract, bifendate group and the model group that embodiment 3 prepares makes hepatic injury mice serum ALT, AST reduce (P<0.05) more to some extent; The each dosage group of Folium Jujubae extract and bifendate group and model group make hepatic injury murine liver tissue MDA level reduce (P<0.05) more to some extent, SOD level rising (P<0.05).Specific experiment the results are shown in Table 3.
Table 3 Folium Jujubae extract causes the impact (mean ± SD, n=10) of acute hepatic injury mice Serum ALT, AST and liver SOD, MDA on ethanol
Note: with model group comparison,
##p<0.01,
#p<0.05.
This experiment adopts CCl
4, 3 kinds of Experimental Mice liver injury models research Folium Jujubaes of D-GalN and ethanol and extract thereof hepatoprotective effect, wherein CCl
4it is the toxic agent of classical chemical liver injury animal model.The pathological change of the pathological change of D-GalN liver injury model and human virus's hepatitis is comparatively approaching, its intoxicating mechanism be in metabolic process with body in phosphoric acid uridine a large amount of in conjunction with forming stable uridine derivatives, cause uridnine in body to exhaust, carry out the material biosynthesis blocks such as biosynthetic RNA, protein and sugar be former thereby make to rely on phosphoric acid uridine, cause hepatic injury even dead.In addition, D-GalN also can cause lipid peroxidation by producing free radical, causes hepatocyte injury.It is the hepar damnification disease of simulation due to long-term heavy drinking that ethanol causes liver injury model.Above experimental result shows, Folium Jujubae provided by the invention and Folium Jujubae extract thereof can significantly reduce CCl
4, liver injury model mice serum ALT, AST activity and Liver MDA due to D-GalN and ethanol; strengthen liver SOD activity; and contrast and experiment shows, the Folium Jujubae extract after the present invention is refining has than the better liver protection of the Folium Jujubae of equivalent.Therefore Folium Jujubae and extract thereof be expected to exploitation become a new generation safe and effective, for preventing and treating medicine or the health product of hepatic injury, and compared with existing medicine, Fructus Jujubae extract is safer, has no adverse reaction.Simultaneously also for Folium Jujubae waste resource is turned waste into wealth and jujube resources comprehensive utilization provides approach.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (2)
1. a Folium Jujubae extract, is characterized in that, prepares by following steps:
(1) get after Folium Jujubae is chosen, cleans, is dried and put into extraction pot, the concentration that adds 6～12 times of amounts is 50%～95% ethanol, is heated to 80 DEG C and refluxes 1～3 time, each continuing refluxed 2 hours, and filtration, merge extractive liquid,, be concentrated into without after alcohol taste, obtain the ethanol extract of Folium Jujubae, for subsequent use;
(2) get the Folium Jujubae ethanol extract that step (1) obtains, add 1～5 times of water gaging, upper D101 type nonpolarity macroporous adsorptive resins chromatographic column, first water and concentration are 10% ethanol elution respectively, the ethanol elution that is 60%～95% by concentration again, collect 60%～95% ethanol elution, concentrated, drying under reduced pressure obtains Folium Jujubae active site crude extract, for subsequent use;
(3) get polyamide column in the Folium Jujubae active site crude extract dry method that step (2) obtains, first distinguish water and 20% ethanol elution, be 70%～95% ethanol elution by concentration again, and collect 70%～95% ethanol elution, concentrated, obtain Folium Jujubae active site extract through drying under reduced pressure;
(4) getting step (3) obtains Folium Jujubae active site extract and adopts preparation liquid phase further enrichment to obtain refining Folium Jujubae extract, after testing, total triterpene and general flavone content are greater than 80%, the chromatographic condition of described preparation liquid phase is: chromatographic column is the anti-phase C18 post of Agilent, mobile phase is methanol-0.5% phosphate aqueous solution, gradient elution, the condition of gradient elution is: 0 to 35 minute, the content of methanol rises to 45% by 5%, the content of 35 to 60 minutes methanol rises to 83% by 45%, detection wavelength is 285nm, flow velocity is 5ml/min, detector is diode array detector.
2. the application of Folium Jujubae extract claimed in claim 1 in preparation control liver injury medicament or health product.
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| CN105232670A (en) * | 2015-11-11 | 2016-01-13 | 王书汉 | Extraction method of effective components of jujube tree leaves and obtained product |
| CN105560438B (en) * | 2015-12-01 | 2019-10-25 | 西北大学 | A method of it extracted from Mel Jujubae, purify phenolic compound |
| CN105687367B (en) * | 2016-01-25 | 2019-11-26 | 西北大学 | A kind of jujube leaf standardized extract and its preparation and application |
| CN105884842B (en) * | 2016-01-28 | 2021-03-12 | 塔里木大学 | Preparation of quercetin-3-O-alpha-L-arabinose- (1 → 2) -alpha-L-rhamnoside from red date leaves and application thereof |
| CN110101731B (en) * | 2019-06-14 | 2021-09-14 | 南京中医药大学 | Chrysanthemum stem and leaf active extract with function of preventing and treating eye diseases and preparation method and application thereof |
| CN114736106B (en) * | 2022-04-28 | 2023-06-09 | 江西中医药大学 | Lignan dimer compound, and preparation method and application thereof |
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| KR100937781B1 (en) * | 2008-09-18 | 2010-01-20 | 화성용 | Preventing liver injury and improving liver function effects of ena-actimineral resources |
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