KR101790185B1 - Cosmetic composition containing extracting fraction of fermented beans as an active ingredient - Google Patents

Cosmetic composition containing extracting fraction of fermented beans as an active ingredient Download PDF

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KR101790185B1
KR101790185B1 KR1020100089363A KR20100089363A KR101790185B1 KR 101790185 B1 KR101790185 B1 KR 101790185B1 KR 1020100089363 A KR1020100089363 A KR 1020100089363A KR 20100089363 A KR20100089363 A KR 20100089363A KR 101790185 B1 KR101790185 B1 KR 101790185B1
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South Korea
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cosmetic composition
fermented
fermented soybean
skin
fraction
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KR1020100089363A
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KR20120027654A (en
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김동현
박준성
장희경
김덕희
김한곤
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(주)아모레퍼시픽
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Priority to PCT/KR2011/006657 priority patent/WO2012036414A2/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Abstract

본 발명은 발효콩 추출 분획물을 유효성분으로 함유하는 화장료 조성물에 관한 것으로, 보다 상세하게는 본 발명의 발효콩 추출 분획물을 함유함으로써 세포 증식 효과 및 세포 재생 효과가 우수한 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition comprising an extract fraction of a fermented soybean as an active ingredient, and more particularly to a cosmetic composition having a cell proliferation effect and a cell regeneration effect by containing the fermented soybean extract fraction of the present invention.

Description

발효콩 추출 분획물을 유효 성분으로 포함하는 화장료 조성물{Cosmetic composition containing extracting fraction of fermented beans as an active ingredient}[0001] The present invention relates to a cosmetic composition containing an extract fraction of fermented soybean as an active ingredient,

본 발명은 발효콩 추출 분획물을 유효성분으로 함유하는 화장료 조성물에 관한 것으로, 보다 상세하게는 본 발명의 발효콩 추출 분획물을 함유함으로써 세포 증식 효과 및 세포 재생 효과가 우수한 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition comprising an extract fraction of a fermented soybean as an active ingredient, and more particularly to a cosmetic composition having a cell proliferation effect and a cell regeneration effect by containing the fermented soybean extract fraction of the present invention.

청국장은 콩을 원료로 한 우리나라의 대표적인 발효식품으로서, 간장, 된장, 고추장 등과 함께 오늘날까지 상용되어 온 전통 장류 중의 하나이며, 곡류를 주식으로 하는 우리민족에게 결핍되기 쉬운 필수아미노산 및 지방산을 공급시켜 주는 식품으로서 중요한 역할을 담당하여 왔다. 청국장은 삶은 콩을 볏짚에 붙어있는 고초균(枯草菌)이라 부르는 바실러스 서브틸리스(Bacillus subtilis)를 이용하여 띄워 만든 것으로서, 발효과정 중에 고초균이 생산하는 효소에 의해서 그 특유의 맛과 냄새를 내는 동시에, 원료 콩의 당질과 단백질에서 유래된 레반형프룩탄(levan form fructan)과 폴리글루타메이트(polyglutamate)의 혼합물질인 점질물을 다량 생성하며, 이렇게 발효 제조된 청국장은 소화 작용뿐만 아니라 영양 면에서도 된장이나 고추장보다 단백질과 지방 함량이 높은 양질의 콩 발효식품이다. 청국장 발효는 바실러스 서브틸리스(Bacillus subtilis)에 의해 이루어지는 것으로 알려져 있으며(Lee et al.,J. Micorbiol. Biotechnol, 13, pp. 892-896, 2003), 상기의 바실러스 서브틸리스(Bacillus subtilis)는 장내부패균의 활동을 약화시키고 병원균에 대한 항균 작용뿐만 아니라, 암모니아, 인돌, 아민 등 발암촉진 물질을 감소시키는 작용을 한다. 특히, 바실러스 서브틸리스(Bacillus subtilis)는 비타민B2를 많이 분비함으로써 간의 해독 기능을 좋게 하여 술, 담배의 뛰어난 해독 효과를 보이며, 유기산의 생성을 통하여 장을 자극하는 등의 반응으로 소화활동 증진 및 면역력을 높여주는 것으로 알려져 있으나 지금까지 발효콩 추출 분획물을 이용하여 세포 재생 화장료 조성물로 개발한 어떠한 내용도 교시되거나 기재된 바 없다.Cheonggukjang is one of the traditional fermented foods of Korea, which is one of the traditional fermented foods that have been used to date with soy sauce, miso, and kochujang, and supplies essential amino acids and fatty acids, which are deficient to Korean people, The state has played an important role as food. Cheonggukjang is made by using Bacillus subtilis called Bacillus subtilis, which is attached to rice straw, and gives its unique taste and smell by the enzyme produced by Bacillus subtilis during fermentation , A large amount of glutinous rice, which is a mixture of levan form fructan and polyglutamate, derived from the saccharides of the raw soybean and protein, and the fermented chonggukjang produced by the fermentation produced not only the digestive action but also the soybean paste It is a high quality soybean fermented food with higher protein and fat content. Bacillus subtilis has been known to be produced by fermentation of Bacillus subtilis (Lee et al., J. Micorbiol. Biotechnol, 13, pp. 892-896, 2003) Weakens the activity of intestinal bacteria and acts not only on the antimicrobial action against pathogens but also on the carcinogenic substances such as ammonia, indole and amine. In particular, Bacillus subtilis secretes a large amount of vitamin B2, thereby enhancing the detoxifying function of the liver, thereby exhibiting excellent detoxification effects of alcohol and tobacco. In addition, by stimulating the intestines through the production of organic acids, It is known that it enhances the immunity. However, any content developed using the fermented soybean extract fraction as a cell regenerating cosmetic composition has not been taught or described.

이에, 본 발명자들은 피부에 안전하면서도 세포 증식 및 세포 재생 효과가 우수하고, 제품으로서의 안정성도 우수한 화장료를 제조하기 위하여 연구하던 중, 발효콩 추출 분획물이 세포 증식 및 세포 재생 효과가 매우 우수한 것을 발견하고 본 발명을 완성하게 되었다.Accordingly, the inventors of the present invention have found that a fermented soybean extract fraction is excellent in cell proliferation and cell regeneration effects, while studying to produce cosmetics which are safe for skin, excellent in cell proliferation and cell regeneration effect, and excellent in stability as a product Thereby completing the present invention.

따라서, 본 발명은 발효콩 추출 분획물을 유효성분으로 함유하여 세포 증식 및 세포 재생 효능이 우수한 화장료 조성물을 제공하는 것을 목적으로 한다.Accordingly, it is an object of the present invention to provide a cosmetic composition containing the fermented soybean extract fraction as an active ingredient and having excellent cell proliferation and cell regeneration efficacy.

상기한 목적을 달성하기 위하여, 본 발명은 발효콩 추출 분획물을 유효성분으로 함유하는 화장료 조성물을 제공한다.In order to achieve the above object, the present invention provides a cosmetic composition comprising an extract fraction of fermented soybean as an active ingredient.

본 발명에 따라 발효콩 추출 분획물을 유효성분으로 함유하는 화장료 조성물은 피부 세포 증식 및 세포 재생 효과가 우수하여 상처 회복을 위한 피부 재생 능력을 향상시킬 수 있고, 피부 재생 능력 감퇴로 인한 피부 탄력 감소 및 피부 노화의 심화를 방지함으로써 우수한 피부 탄력 개선 효과 및 피부 노화 방지 효과를 나타낸다. The cosmetic composition containing the fermented soybean extract fraction as an active ingredient according to the present invention is excellent in skin cell proliferation and cell regeneration effect and can improve skin regeneration ability for wound healing, Thereby preventing the deepening of skin aging and thereby exhibiting excellent skin elasticity improvement effect and skin aging prevention effect.

도 1은 발효콩 추출 분획물, 발효콩 추출물의 세포 독성 평가를 측정한 그래프이다.
도 2는 발효콩 추출 분획물의 인간 섬유 아세포에서의 세포 재생 효과를 나타낸 그래프이다.
도 3은 발효콩 추출 분획물의 HaCaT 세포에서의 세포 재생 효과를 나타낸 그래프이다.FIG. 1 is a graph showing cytotoxicity evaluation of fermented soybean extract fractions and fermented soybean extract. FIG.
2 is a graph showing the cell regeneration effect of the fermented soybean extract fraction in human fibroblasts.
3 is a graph showing cell regeneration effect of HaCaT cells in fermented soybean extract fractions.

본 발명은 발효콩 추출 분획물을 유효성분으로 함유하는 화장료 조성물을 제공한다.The present invention provides a cosmetic composition comprising a fermented soybean extract fraction as an active ingredient.

이하, 본 발명을 보다 구체적으로 설명한다.
Hereinafter, the present invention will be described more specifically.

일반적인 추출법에 의해 추출한 뒤 여과 및 농축하여 제조되는 발효콩 추출물과는 달리, 본 발명에 따른 발효콩 추출 분획물은 농축된 상태의 발효콩 추출물을 유기용매, 예를 들어 에탄올로 한번 더 추출하여 여과한 후, 막 필터(membrane filter)를 이용한 원심분리를 통해 분리되어 나온 여액을 감압 농축하여 수득할 수 있다.Unlike the fermented soybean extract prepared by extracting by the general extraction method, followed by filtration and concentration, the fermented soybean extract fraction according to the present invention is obtained by once extracting the concentrated fermented soybean extract with an organic solvent, for example, ethanol, The filtrate separated by centrifugation using a membrane filter can be obtained by concentration under reduced pressure.

본 발명의 발효콩 추출 분획물은 3kDa~10kDa의 분자량을 갖는 것을 특징으로 한다. 3kDa 미만의 분자량에서는 세포 독성을 나타내기 때문에 바람직하지 않다.The fermented soybean extract fraction of the present invention is characterized by having a molecular weight of 3 kDa to 10 kDa. And molecular weights below 3 kDa are undesirable because they exhibit cytotoxicity.

본 발명의 화장료 조성물은 발효콩 추출 분획물을 조성물 총 중량에 대하여 0.0001~30중량%, 보다 바람직하게는 0.1~10중량%의 양으로 함유한다. 그 함량이 0.0001중량% 미만이면 유효성분에 의한 세포 증식 및 세포 재생 효과 등을 얻을 수 없고, 30중량% 초과이면 함량 증가에 비해 효과의 증가가 크지 않기 때문에 바람직하지 않다.The cosmetic composition of the present invention contains the fermented soybean extract fraction in an amount of 0.0001 to 30% by weight, more preferably 0.1 to 10% by weight based on the total weight of the composition. If the content is less than 0.0001% by weight, cell proliferation and cell regeneration effect by the active ingredient can not be obtained. If the content is more than 30% by weight, the increase of the effect is not so large compared to the increase of the content.

본 발명에 의한 발효콩 추출 분획물은 그 사용에 특별히 제한되지 않으며, 예를 들면, 화장료 조성물 이외에도 약제 조성물 등에 사용될 수 있다.The fermented soybean extract fraction according to the present invention is not particularly limited in its use, and can be used, for example, in pharmaceutical compositions in addition to cosmetic compositions.

본 발명의 화장료 조성물의 제형은 특별히 제한되지 않으며, 목적하는 바에 따라 적절히 선택할 수 있다. 예를 들어, 본 발명의 화장료 조성물은 유연화장수, 영양화장수, 마사지크림, 에센스, 영양크림, 팩, 피부외용연고 등의 제형으로 제조될 수 있다.The formulation of the cosmetic composition of the present invention is not particularly limited and can be appropriately selected according to the purpose. For example, the cosmetic composition of the present invention can be manufactured into a formulation such as a softening longevity, a nutritional lotion, a massage cream, an essence, a nutritional cream, a pack, and an external ointment for skin.

이하, 본 발명을 실시예 및 시험예를 들어 보다 상세히 설명하겠지만, 본 발명이 이들 예로만 한정되는 것은 아니다.
Hereinafter, the present invention will be described in more detail with reference to examples and test examples, but the present invention is not limited to these examples.

[제조예 1] 발효 청국장 추출물의 제조[Preparation Example 1] Preparation of fermented Chungkukjang extract

발효 청국장 1 kg에 물 5 ℓ를 넣고, 3회 환류 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 분리하고, 분리된 여액을 감압 농축하여 발효 청국장 추출물 350g을 얻었다.
5 kg of water was added to 1 kg of fermented Chongkookjang, refluxed three times, and then immersed at 15 ° C for 1 day. Thereafter, the residue and filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to obtain 350 g of a fermented Chungkookjang extract.

[제조예 2] 발효 청국장 추출 분획물의 제조[Preparation Example 2] Preparation of fermented chongkukjang extract fraction

상기 제조예 1의 발효 청국장 추출물 100g을 EtOH 3ℓ에 넣고, 3회 환류 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 분리하고, 분리된 여액을 UF(Ultra-Filtration) 키트에 넣은 뒤 원심분리를 통해 3kDa 이상의 분자량을 가지는 샘플로 분리한 뒤 분리된 여액을 감압 농축하여 발효 청국장 추출 분획물 33g을 얻었다.
100 g of the fermented Chungkukjang extract of Preparation Example 1 was added to 3 L of EtOH, refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was put into a UF (Ultra-Filtration) kit and centrifuged to separate into samples having a molecular weight of 3 kDa or more. And concentrated to obtain 33 g of a fermented chongkukjang extract fraction.

[시험예 1] 세포 독성 평가(MTT assay)[Test Example 1] Cytotoxicity evaluation (MTT assay)

시험 물질의 세포 독성을 평가하기 위해 HaCaT 10000 세포수/웰을 96-웰 플레이트에 심었다. 24시간 배양 후 FBS가 들어있지 않은 배지에서 24시간 배양하였다. 배양 후 배지를 제거하고 PBS를 100㎕씩 넣어준 후에 UVB 40mJ을 조사하였다. 이때 UVB를 조사하지 않는 실험군도 만든다. 물질을 농도별로 처리하여 48시간 동안 배양 후, MTT 용액(solution)(0.5mg/mL)을 100㎕씩 넣어주고 3시간 동안 37℃의 배양기에서 배양하였다. 상기 용액을 제거하면 포르마잔(formazan)이 형성되는데 DMSO를 100㎕ 넣어 주고 녹인 후, 570nm에서의 흡광도를 측정하여 세포독성을 확인하였다. 그 결과는 도 1에 나타내었다.To evaluate the cytotoxicity of the test material, HaCaT 10000 cells / well were planted in 96-well plates. After 24 hours of culture, the cells were cultured in a medium containing no FBS for 24 hours. After the incubation, the medium was removed, 100 μl of PBS was added, and UVB was irradiated at 40 mJ. At this time, an experiment group that does not irradiate UVB is also made. Subsequently, MTT solution (0.5 mg / mL) was added to each well and incubated for 3 hours at 37 ° C. When the solution was removed, formazan was formed. DMSO (100 μl) was added and dissolved, and the absorbance at 570 nm was measured to confirm cytotoxicity. The results are shown in Fig.

도 1로부터 알 수 있듯이, 본 발명에 따른 발효콩 추출 분획물은 세포 독성이 적으면서 세포 증식 효능이 우수함을 확인할 수 있었다. 반면, 발효콩 추출물은 발효콩 추출 분획물과 비교하여 세포 독성이 크며, 농도에 비례하여 세포 독성도 증가함을 알 수 있었다.
As can be seen from FIG. 1, the fermented soybean extract fraction according to the present invention was found to be excellent in cell proliferation efficiency with low cytotoxicity. On the other hand, fermented soybean extracts showed higher cytotoxicity compared to fermented soybean extract fractions and increased cytotoxicity in proportion to concentration.

[시험예 2] 세포 재생 평가(Cell Proliferation assay) [Test Example 2] Cell proliferation assay

시험 물질의 세포 재생 효과를 알아보기 위해 HaCaT 10000세포수/웰, 정상 인간 섬유아세포(normal human fibroblast) 6000 세포수/웰로　96-웰 플레이트에 심었다. 물질을 처리하기 전에 배지를 FBS가 첨가되지 않은 배지로 교환하고 1시간 동안 배양하였다. 이후 배지를 제거하고 FBS가 1% 함유된 배지에 상기 제조예 2의 발효 청국장 추출 분획물을 0.1, 1, 5, 10, 50, 100ppm의 농도별로 녹인 후 처리하였다. 이때 10% FBS가 함유된 배지는 양성대조군으로 사용하였다. 상기 제조예 2의 발효 청국장 추출 분획물 처리 후 48시간 동안 배양 후, WST-1을 100㎕/웰로 처리하였다.　WST-1을 처리하고 3시간 동안 37℃ 5% CO2 배양기에서 반응시켰다. 3시간 동안 배양 후 마이크로플레이트 리더기(microplate reader)를 이용하여 450nm 에서 흡광도를 측정하여 세포 재생　효과를 확인하였다. 그 결과는 도 2~3에 나타내었다. To investigate the cell regeneration effect of the test substance, HaCaT 10000 cells / well and normal human fibroblast 6000 cells / well were seeded in a 96-well plate. Before processing the material, the medium was exchanged with the medium without FBS and incubated for 1 hour. Then, the medium was removed and the fermented chongkukjang extract fraction of Preparation Example 2 was dissolved in the concentrations of 0.1, 1, 5, 10, 50, and 100 ppm in a medium containing 1% FBS. The medium containing 10% FBS was used as a positive control. After culturing for 48 hours after treatment with the fermented Chongkukjang extract fraction of Preparation Example 2, WST-1 was treated at 100 쨉 l / well. WST-1 was treated and reacted in a 37 ° C 5% CO 2 incubator for 3 hours. After incubation for 3 hours, the absorbance was measured at 450 nm using a microplate reader to confirm cell regeneration effect. The results are shown in Figs.

도 2~3으로부터 본 발명에 따른 발효콩 추출 분획물은 두 가지 세포 실험군에 대해 1% FBS 대비 유의적인 세포 재생 효능을 확인할 수 있었다.
2 to 3, the fermentation soybean extract fraction according to the present invention was able to confirm significant cell regeneration efficiency relative to 1% FBS in the two cell test groups.

[시험예 3] 피부 탄력 개선 효과[Test Example 3] Skin elasticity improvement effect

하기 표 1의 조성을 갖는 실시예 1~2 및 비교예 1~2의 화장수 제형을 제조하였다.The cosmetic formulations of Examples 1 and 2 and Comparative Examples 1 and 2 having the compositions shown in the following Table 1 were prepared.

성분명Ingredients 실시예 1Example 1 실시예 2Example 2 비교예 1Comparative Example 1 비교예 2Comparative Example 2 발효 청국장 추출 분획물
(상기 제조예 2)Fermented chongkukjang extract fraction
(Preparation Example 2) 0.50.5 1010 -- -- 발효 청국장 추출물
(상기 제조예 1)Fermented Chungkukjang extract
(Preparation Example 1) -- -- 0.50.5 -- 세테아릴글루코사이드Cetearyl glucoside 1.51.5 1.51.5 1.51.5 1.51.5 세틸옥타노에이트Cetyl octanoate 3.03.0 3.03.0 3.03.0 3.03.0 메틸파라벤Methyl paraben 0.150.15 0.150.15 0.150.15 0.150.15 에칠렌디아민테트라초산디나트륨Disodium ethylenediaminetetraacetate 0.020.02 0.020.02 0.020.02 0.020.02 정제수Purified water to 100to 100 to 100to 100 to 100to 100 to 100to 100

상기 실시예 1~2 및 비교예 1~2에서 제조된 화장수에 대하여 피부 탄력 향상 효과를 비교하기 위하여, 30∼40대 여성 80명을 각각 20명씩 4개군으로 나누어 측정하였다. 실험 방법은 다음과 같다.In order to compare skin elasticity improvement effects of the lotion prepared in Examples 1 and 2 and Comparative Examples 1 and 2, 80 women in their 30s and 40s were divided into 4 groups each consisting of 20 individuals. The experimental method is as follows.

먼저, 각 군에 실시예 1~2 및 비교예 1~2에서 제조된 화장수를 주고 매일 1회 12주간 눈가에 일정량을 도포하게 하였다. 12주 뒤 피부탄력을 측정할 수 있는 큐토미터(cutometer; SEM474, Courage+Khazaka electronic GmbH. Germany)를 이용하여 각 군의 피부 탄력을 측정하고 그 평균을 구하였다. 그 실험 결과는 하기 표 2에 나타내었다.First, the skin lotion prepared in Examples 1 and 2 and Comparative Examples 1 and 2 was applied to each group, and a predetermined amount was applied to the eyeballs once a day for 12 weeks. After 12 weeks, the skin elasticity of each group was measured using a cutometer (SEM474, Courage + Khazaka electronic GmbH, Germany) capable of measuring the elasticity of the skin, and the skin elasticity was averaged. The experimental results are shown in Table 2 below.

구분division 피부탄력향상도(%)Skin elasticity improvement (%) 실시예 1Example 1 26.826.8 실시예 2Example 2 33.633.6 비교예 1Comparative Example 1 1.11.1 비교예 2Comparative Example 2 0.40.4

상기 표 2에서 알 수 있듯이, 본 발명에 따른 발효콩 추출 분획물을 함유하는 화장료 조성물(실시예 1~2)은 피부 탄력을 향상시켰으며, 함량이 증가함에 따라 피부 탄력 향상도가 높아졌다. 반면, 일반적인 발효콩 추출물을 함유하는 화장료 조성물(비교예 1) 및 발효콩 추출물을 함유하지 않는 화장료 조성물(비교예 2)는 상기 실시예 1~2와 비교하여 피부 탄력 향상 효과가 낮음을 확인할 수 있었다.
As can be seen from Table 2, the cosmetic composition containing the fermented soybean extract fractions according to the present invention (Examples 1 and 2) improved skin elasticity and increased skin elasticity with increasing content. On the other hand, the cosmetic composition (Comparative Example 1) containing a general fermented soybean extract and the cosmetic composition containing no fermented soybean extract (Comparative Example 2) showed less skin elasticity-improving effect than the above-mentioned Examples 1 and 2 there was.

[시험예 4] 피부 주름 개선 효과[Test Example 4] Effect of improving skin wrinkles

상기 실시예 1~2 및 비교예 1~2의 피부 주름 개선 효과를 확인하기 위하여 다음과 같이 실험을 실시하였다.In order to confirm the skin wrinkle improving effect of Examples 1 and 2 and Comparative Examples 1 and 2, the following experiment was conducted.

30대 여성 80명을 각각 실시예 1~2 및 비교예 1~2군으로 20명씩 4개군으로 나누어 매일 1회 8주간 도포한 후, 실리콘을 이용하여 레플리카를 떠서 주름의 상태를 비지오미터(visiometer)로 측정하여 화상분석하고, 그 결과를 하기 표 3에 나타내었다. 표 3의 결과는 도포 8주 후의 각각의 파라미터(parameter) 값에서 도포 전 파라미터 값을 뺀 것의 평균이다.80 women in their 30s were divided into 4 groups of 20 persons each in Examples 1 and 2 and Comparative Examples 1 and 2, and applied once a day for 8 weeks. Then, the replicas were removed using silicone to measure the state of the wrinkles with a visiometer ), And the results are shown in Table 3 below. The results in Table 3 are an average of values obtained by subtracting the pre-application parameter values from the respective parameter values after 8 weeks of application.

사용 8주 후 임상 결과Clinical results after 8 weeks of use R1R1 R2R2 R3R3 R4R4 R5R5 실시예 1Example 1 -0.18-0.18 -0.14-0.14 -0.08-0.08 -0.06-0.06 -0.05-0.05 실시예 2Example 2 -0.27-0.27 -0.20-0.20 -0.10-0.10 -0.08-0.08 -0.06-0.06 비교예 1Comparative Example 1 -0.08-0.08 -0.07-0.07 -0.03-0.03 -0.02-0.02 -0.03-0.03 비교예 2Comparative Example 2 0.030.03 0.050.05 0.020.02 -0.01-0.01 0.010.01 R1: 주름 등고선의 최고치와 최저치의 차이값
R2: 주름 등고선을 임의로 5칸씩 나눈 후 그 중 R1값 들의 평균
R3: 5개씩 나눈 R1값 중 최고치
R4: 주름 등고선의 baseline에서 각 각의 꼭대기와 계곡의 값을 뺀 평균값
R5: 주름 등고선의 baseline에서 각 각의 주름 윤곽을 뺀 값의 차이 값R1: Difference between the maximum value and the minimum value of the wrinkle contour line
R2: The wrinkle contour line is arbitrarily divided into 5 squares, and the average of R1 values
R3: Maximum value of R1 divided by 5
R4: Average value obtained by subtracting the value of the apex and valley of each angle from the baseline of the wrinkle contour line
R5: Difference between the baseline of the wrinkle contour line minus the outline of each wrinkle

상기 표 3에서 알 수 있듯이, 본 발명에 따른 발효콩 추출 분획물을 함유하는 화장료 조성물(실시예 1~2)은 피부 주름을 개선시켰으며, 함량이 증가함에 따라 피부 주름 개선 효과가 높아졌다. 반면, 일반적인 발효콩 추출물을 함유하는 화장료 조성물(비교예 1) 및 발효콩 추출물을 함유하지 않는 화장료 조성물(비교예 2)는 상기 실시예 1~2와 비교하여 피부 주름 개선 효과가 낮음을 확인할 수 있었다.As can be seen from the above Table 3, the cosmetic composition containing the fermented soybean extract fraction according to the present invention (Examples 1 and 2) improved skin wrinkles, and skin wrinkles were improved with an increase in content. On the other hand, the cosmetic composition (Comparative Example 1) containing a general fermented soybean extract and the cosmetic composition (Comparative Example 2) containing no fermented soybean extract showed less effect of improving skin wrinkles as compared with Examples 1 and 2 there was.

하기에 상기 조성물의 제형예들을 설명하나, 본 발명은 이를 한정하고자 함이 아니라 단지 구체적으로 설명하고자 함이다. Formulation examples of the composition will be described below, but the present invention is not intended to be limited thereto but is specifically described.

[제형예 1] 유연화장수[Formulation Example 1] Flexible longevity

성분명Ingredients 함량(중량%)Content (% by weight) 발효콩 추출 분획물Fermented soybean extract fraction 0.50.5 부틸렌글리콜Butylene glycol 2.02.0 프로필렌글리콜Propylene glycol 2.02.0 카르복시비닐폴리머Carboxyvinyl polymer 0.10.1 피이지-12 노닐페닐에테르Phage-12 nonylphenyl ether 0.20.2 폴리솔베이트 80Polysorbate 80 0.40.4 에탄올ethanol 10.010.0 트리에탄올아민Triethanolamine 0.10.1 방부제, 색소 및 향료Preservatives, coloring and flavoring 적량Suitable amount 정제수Purified water to 100to 100

[제형예 2] 영양화장수[Formulation Example 2] Nutritional lotion

성분명Ingredients 함량(중량%)Content (% by weight) 발효콩 추출 분획물Fermented soybean extract fraction 0.50.5 밀납Wax 4.04.0 폴리솔베이트 60Polysorbate 60 1.51.5 솔비탄세스퀴올레이트Sorbitan sesquioleate 1.51.5 유동파라핀Liquid paraffin 0.50.5 카프릴릭/카프릭트리글리세라이드Caprylic / capric triglyceride 5.05.0 글리세린glycerin 3.03.0 부틸렌글리콜Butylene glycol 3.03.0 프로필렌글리콜Propylene glycol 3.03.0 카르복시비닐폴리머Carboxyvinyl polymer 0.10.1 트리에탄올아민Triethanolamine 0.20.2 방부제, 색소 및 향료Preservatives, coloring and flavoring 적량Suitable amount 정제수Purified water to 100to 100

[제형예 3] 영양크림[Formulation Example 3] Nourishing cream

성분명Ingredients 함량(중량%)Content (% by weight) 발효콩 추출 분획물Fermented soybean extract fraction 0.50.5 밀납Wax 10.010.0 폴리솔베이트 60Polysorbate 60 1.51.5 피이지 60 경화피마자유Piggy 60 hardened castor oil 2.02.0 솔비탄세스퀴올레이트Sorbitan sesquioleate 0.50.5 유동파라핀Liquid paraffin 10.010.0 스쿠알란Squalane 5.05.0 카프릴릭/카프릭트리글리세라이드Caprylic / capric triglyceride 5.05.0 글리세린glycerin 5.05.0 부틸렌글리콜Butylene glycol 3.03.0 프로필렌글리콜Propylene glycol 3.03.0 트리에탄올아민Triethanolamine 0.20.2 방부제, 색소 및 향료Preservatives, coloring and flavoring 적량Suitable amount 정제수Purified water to 100to 100

[제형예 4] 마사지크림[Formulation Example 4] Massage cream

성분명Ingredients 함량(중량%)Content (% by weight) 발효콩 추출 분획물Fermented soybean extract fraction 0.50.5 밀납Wax 10.010.0 폴리솔베이트 60Polysorbate 60 1.51.5 피이지 60 경화피마자유Piggy 60 hardened castor oil 2.02.0 솔비탄세스퀴올레이트Sorbitan sesquioleate 0.80.8 유동파라핀Liquid paraffin 40.040.0 스쿠알란Squalane 5.05.0 카프릴릭/카프릭트리글리세라이드Caprylic / capric triglyceride 4.04.0 글리세린glycerin 5.05.0 부틸렌글리콜Butylene glycol 3.03.0 프로필렌글리콜Propylene glycol 3.03.0 트리에탄올아민Triethanolamine 0.20.2 방부제, 색소 및 향료Preservatives, coloring and flavoring 적량Suitable amount 정제수Purified water to 100to 100

[제형예 5] 팩[Formulation Example 5] Pack

성분명Ingredients 함량(중량%)Content (% by weight) 발효콩 추출 분획물Fermented soybean extract fraction 0.50.5 폴리비닐알콜Polyvinyl alcohol 13.013.0 소듐카르복시메틸셀룰로오스Sodium carboxymethylcellulose 0.20.2 글리세린glycerin 5.05.0 알란토인Allantoin 0.10.1 에탄올ethanol 6.06.0 핑지-12 노닐페닐에테르Fingi-12 nonylphenyl ether 0.30.3 폴리솔베이트 60Polysorbate 60 0.30.3 방부제, 색소 및 향료Preservatives, coloring and flavoring 적량Suitable amount 정제수Purified water to 100to 100

Claims (8)

발효콩 추출 분획물을 유효성분으로 함유하는 피부 탄력 및 주름 개선용 화장료 조성물로서,
상기 발효콩 추출 분획물은 발효 청국장의 수(水) 추출물을 환류 조건 하에서 에탄올로 재추출한 다음, 한외여과(Ultra-Filtration)에 의해 원심분리한 뒤 감압 농축하여 제조한 분획물이며, 3 내지 10 kDa의 분자량을 갖는 것을 특징으로 하는, 피부 탄력 및 주름 개선용 화장료 조성물.A cosmetic composition for improving skin elasticity and wrinkles containing an extract fraction of fermented soybean as an active ingredient,
The fermented soybean extract fraction is a fraction prepared by re-extracting the water extract of fermented Chungkukjang with ethanol under refluxing condition, centrifuging it by ultra-filtration and concentrating it under reduced pressure, and having a concentration of 3 to 10 kDa A cosmetic composition for improving skin elasticity and wrinkles, which has a molecular weight. 제 1항에 있어서, 상기 발효콩 추출 분획물의 함량은 조성물 총 중량에 대하여 0.0001 내지 30 중량%임을 특징으로 하는, 피부 탄력 및 주름 개선용 화장료 조성물.The cosmetic composition for improving skin elasticity and wrinkles according to claim 1, wherein the content of the fermented soybean extract fraction is 0.0001 to 30% by weight based on the total weight of the composition. 제 1항에 있어서, 상기 발효 청국장의 수(水) 추출물은 발효 청국장을 환류 조건 하에서 물로 추출하여, 여과포 여과와 원심분리를 통해 잔사와 여액을 분리하고, 분리된 여액을 감압 농축하여 수득한 것임을 특징으로 하는, 피부 탄력 및 주름 개선용 화장료 조성물.The method according to claim 1, wherein the water extract of the fermented chongkukjang is obtained by extracting fermented chongkukjang with water under reflux conditions, separating the residue and the filtrate by filtration and centrifugation through a filter cloth, and concentrating the separated filtrate under reduced pressure Wherein the skin elasticity and wrinkle are improved. 제 1항, 제 2항, 또는 제 3항에 있어서, 상기 조성물은 유연화장수, 영양화장수, 마사지크림, 에센스, 영양크림, 팩, 피부외용연고로 제형화되는 피부 탄력 및 주름 개선용 화장료 조성물.The cosmetic composition according to claim 1, 2, or 3, wherein the composition is formulated into an ointment for softening, a nutritional lotion, a massage cream, an essence, a nutritional cream, a pack, and an external ointment for skin. 발효콩 추출 분획물을 유효성분으로 함유하는 피부 세포 재생용 화장료 조성물로서,
상기 발효콩 추출 분획물은 발효 청국장의 수(水) 추출물을 환류 조건 하에서 에탄올로 재추출한 다음, 한외여과(Ultra-Filtration)에 의해 원심분리한 뒤 감압 농축하여 제조한 분획물이며, 3 내지 10 kDa의 분자량을 갖는 것을 특징으로 하는, 피부 세포 재생용 화장료 조성물.A cosmetic composition for skin cell regeneration comprising an extract fraction of fermented soybean as an active ingredient,
The fermented soybean extract fraction is a fraction prepared by re-extracting the water extract of fermented Chungkukjang with ethanol under refluxing condition, centrifuging it by ultra-filtration and concentrating it under reduced pressure, and having a concentration of 3 to 10 kDa Wherein the cosmetic composition has a molecular weight. 제 5항에 있어서, 상기 발효콩 추출 분획물의 함량은 조성물 총 중량에 대하여 0.0001 내지 30 중량%임을 특징으로 하는, 피부 세포 재생용 화장료 조성물.6. The cosmetic composition for skin cell regeneration according to claim 5, wherein the content of the fermented soybean extract fraction is 0.0001 to 30% by weight based on the total weight of the composition. 제 5항에 있어서, 상기 발효 청국장의 수(水) 추출물은 발효 청국장을 환류 조건 하에서 물로 추출하여, 여과포 여과와 원심분리를 통해 잔사와 여액을 분리하고, 분리된 여액을 감압 농축하여 수득한 것임을 특징으로 하는, 피부 세포 재생용 화장료 조성물.[Claim 6] The method according to claim 5, wherein the water extract of the fermented chungkukjang is obtained by extracting the fermented chongkukjang with water under reflux conditions, separating the residue and the filtrate through filtration and centrifugation of the filter cloth and concentrating the separated filtrate under reduced pressure Wherein said skin cell regeneration composition is a cosmetic composition for skin cell regeneration. 제 5항, 제 6항, 또는 제 7항에 있어서, 상기 조성물은 유연화장수, 영양화장수, 마사지크림, 에센스, 영양크림, 팩, 피부외용연고로 제형화되는 피부 세포 재생용 화장료 조성물.The cosmetic composition for skin cell regeneration according to claim 5, 6, or 7, wherein the composition is formulated into softening water, nutritional lotion, massage cream, essence, nutritional cream, pack, ointment for skin.
KR1020100089363A 2010-09-13 2010-09-13 Cosmetic composition containing extracting fraction of fermented beans as an active ingredient KR101790185B1 (en)

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