KR101875188B1 - Functional cosmetic composition comprising tengcha - Google Patents

Abstract

The present invention relates to a functional cosmetic composition using Tengcha. More specifically, the cosmetic composition comprises 0.1-10 wt% of an extract of Tengcha as an active ingredient based on the total weight of the entire composition. According to the present invention, skin, hair and scalp of a user can be maintained healthily through antioxidative, moisturizing, antibacterial, elasticity enhancing, whitening, pore improving, and wrinkle alleviating functions of Tengcha.

Description

{FUNCTIONAL COSMETIC COMPOSITION COMPRISING TENGCHA}

TECHNICAL FIELD The present invention relates to a cosmetic composition, and more particularly, to a functional cosmetic composition using an isoquant, which has an action of improving the skin condition.

Clear, transparent, and white skin is the skin-oriented point that all women dream of. Skin is the largest organ of the body, always in direct contact with the external environment, and serves as a protective layer to protect the body from various stimuli or dry environment. It is a vital organ for the generation and destruction of new cells compared to the organ. It protects the body from physical abrasion, protects the inside of the body from moisture loss, protects it from ultraviolet rays generated from the sun, It is responsible for various physical factors, external environmental factors, infection, etc., which causes skin irritation such as skin contamination, drying, trouble and pigmentation.

In other words, it is the largest organ of the human body and plays an important role in various roles such as protection function, barrier function, temperature control function, excretion function and respiration function. As skin age, skin function is rapidly deteriorated, The thickness of the skin is thinned and the moisture content of the skin is lowered, and the skin becomes dry, causing a variety of skin lesions such as wrinkles, spots, freckles, skin elasticity reduction and pigmentation.

Accordingly, there is a growing demand for a functional cosmetic composition that imparts cosmetic effects such as whitening, wrinkle prevention, moisturizing, elasticity, and softening effect to the skin.

As an example of such a functional cosmetic composition, Korean Patent Registration No. 10-1274778 discloses a cosmetic composition prepared by using the extruded wood and herbal extract. Korean Patent Laid-Open No. 10-2013-0075962 discloses a cosmetic composition having a skin-soothing and ultraviolet-shielding function containing an outdoors extract.

However, since the cosmetic composition has different functions and effects depending on the individual taste and skin type, the demand for other types of functional cosmetic compositions other than the above-mentioned composition is increasing.

KR 10-1274778 B1 KR 10-2013-0075962 A

Accordingly, an object of the present invention is to provide a functional cosmetic composition having whitening, moisturizing, antibacterial, elasticity-enhancing, pore-improving and wrinkle-improving functions by using an extract of Ishikari.

Another object of the present invention is to improve the condition of the user's skin, scalp and hair through extracts of Isostar.

In order to achieve the above object, the functional cosmetic composition of the present invention using isoquinolone comprises 0.1 to 10% by weight of an isostatic extract as an active ingredient based on the total weight of the whole composition.

And 0.1 to 5% by weight of an equal-weight fermentation extract.

And 0.1 to 5% by weight of a cacao bean extract, 0.1 to 5% by weight of a waxy extract, 0.1 to 5% by weight of a perilla leaf extract, 0.1 to 5% by weight of a calamansi extract and 0.1 to 5% by weight of a cacao bean extract.

The composition can be used as a cosmetic or dermatological composition in the form of a lotion such as a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a cleansing oil, a powder cleansing, a cosmetic pack, a face scrub, Lip Gloss, Lip Gloss, Lip Balm, Lip Liner, Brow Pencil, Brow Color, Mascara, Eye Shadow, Eyeliner, Ball Touch, Highlight, Foundation, Base, Powder, Fact, BB Cream, CC Cream, CB Cream, Powder, Shampoo, Body Which is one of the following types of cosmetics: shampoo, rinse, scalp massage agent, hair treatment, body lotion, hand wash, body scrub, body oil, shower cologne, body cleanser, soap, perfume, skin itching relief, .

According to the present invention, the skin, hair, and scalp of a user can be maintained healthily through antioxidation, moisturizing, antibacterial, elasticity enhancement, whitening, pore improvement, and wrinkle improvement functions.

Hereinafter, the present invention will be described in detail.

The present invention is to provide a functional cosmetic composition which imparts functionality to a cosmetic composition by utilizing an extract of Ishida. Here, the function means whitening, moisturizing, anti-inflammation, elasticity enhancement, pore improvement, wrinkle improvement, and the like.

The functional cosmetic composition according to the present invention is characterized in that it contains an equal amount of extract in an amount of 0.1 to 10% by weight based on the total weight of the composition.

Here, the tengcha (Ampelopsis grossedentata) is a kind of wild stem plant of grape and beetle, which is known as moyeam. Most of the above-mentioned islands grow in Hongsam, Mt. Monsan, 800 ~ 1500m high, and germinate in spring and fade in autumn. Stems and leaves have no hairs. Stem diameter is about 0.2 ~ 0.4cm. The biggest annual output is early summer The next is autumn. This is a rare and rare treasure missing from the main river gem. It contains 17 kinds of amino acids such as leucine, isoserine and methionine and 14 kinds of trace elements such as potassium, calcium, zinc and selenium. Dihydromyricetin (DMY) , anthocyan (OPC), and flavone (Flavone) are very high. More specifically, the above equilibrium has the highest flavonoid content among the currently found plants with a flavon content of 7.5-8.3% (minimum content ≥6%), crude protein content is 12.8-13.8%, and Dihydromyricetin content is about 25% It reaches.

In the present invention, the stem and the leaves of such isomorphs are collected and extracted and used. When the extract of this isomalt is included in the cosmetic composition, excellent whitening, moisturizing, antibacterial, elasticity enhancement, Because.

In the present invention, the extraction of the equipotential may be performed by hot water extraction, cold extraction, warming extraction, reflux cooling extraction, ultrasonic extraction, or the like. In addition, the extraction is carried out using a solvent, and water, ethanol, or a mixed solvent thereof may be used as the solvent.

More specifically, the extraction is carried out by adding a dry weight of 3 to 20 times by weight of a solvent to the dried powder of the stem, the leaves or the mixture of the isomorphs and drying the solution at 20 to 100 ° C, more preferably at an extraction temperature of 40 to 90 ° C For 30 minutes to 5 days, more preferably for 1 to 24 hours. It also includes, if necessary, concentrating, lyophilizing and pulverizing. Here, the lyophilization follows a known method in the field to which this technique belongs, and thus a detailed description thereof will be omitted.

The dry powder refers to a product obtained by picking up the stem and leaves of the isola and drying it through a method such as a hair dryer, shade, hot air drying, etc., and then, It is sufficient if it is crushed to 50 ~ 300mesh as an example. In addition, it may be crushed and dried, followed by shredding.

In the present invention, it is preferable that the extract of the present invention contains 0.1 to 10% by weight, based on the total weight of the cosmetic composition. If the content is too small, the antibacterial activity, antioxidant activity, whitening, If the amount is too much, the increase of the effect of the increase of the content is very weak and the stability of the formulation can not be secured.

In addition, the cosmetic composition of the present invention may further comprise 0.1 to 5% by weight of an isosteric fermentation extract. When the fermented extract of the present invention is further contained, the functional properties of the cosmetic composition, antioxidation, antibacterial activity, whitening, Because.

In this case, the fermentation extract of the present invention is prepared by adding water and saccharide to the above-described extract of the order tea at a weight ratio of 1: 1 to 10: 0.1 to 0.2, and inoculating the fermentation microorganism with the amount of 100,000 to 10,000,000 cfu / L Followed by fermentation at 20 to 40 ° C for 12 to 60 hours. The fermented fermentation broth is centrifuged to separate only the supernatant. The supernatant may be dried and used. When the extract is dried, it is extracted with an extraction solvent of 1 to 20 times its dry weight, and is then immersed at 4 to 30 ° C for 3 to 20 days to extract an active ingredient. The filtrate is concentrated under reduced pressure And lyophilization. The extraction by the extraction solvent may be performed using any extraction solvent conventionally used in the art, but extraction is performed using at least one solvent selected from the group consisting of water and ethanol.

At this time, it is preferable to use Saccharomyces genus as the fermenting microorganism, but Saccharomyces cerevisiae ATCC 18824 is more preferably used. As the sugar, , Oligosaccharides, and the like.

In addition, the cosmetic composition of the present invention may further comprise 0.1-5% by weight of the extract of Wakoshi and 0.1-5% by weight of P. forest leaf extract, based on the total weight of the whole composition.

The above extracts of Wakoshi and P. forest leaf extracts have excellent antioxidation, moisturizing and skin-soothing properties.

The wort extract may be prepared by extracting dried leaves, leaves or mixed powders thereof with hot water extraction, cold extraction, warm-up extraction, reflux cooling or the like using water, ethanol or a mixed solvent thereof, Extraction, and ultrasonic extraction.

Here, the dried dried powder can be dried in a known manner to such an extent that the useful components thereof are not destroyed. For example, the dried dried powder can be dried by a method of natural drying in a shade, It is enough if it is crushed to such an extent that it can be extracted. In addition, the drying and crushing processes may be carried out in reverse order or repeatedly, if necessary, and may be omitted.

If the content of the dried persimmon extract is less than 0.1% by weight, the effect thereof is insignificant. If the content of the persimmon extract is too large, the increase in the effect due to the increase of the content is insignificant and the stability on the formulation can not be secured. By weight based on the total weight of the composition.

In the same manner as the extraction method of the above-mentioned extract of the order tree, the extracts of the perennial apricot can be obtained by hot water extraction, cold extraction, warm-up extraction, reflux cooling extraction, ultrasound extraction using water, ethanol or a mixed solvent thereof It is extracted.

At this time, the dried powder of the perennial apricot leaf is dried by a known method within a range that useful components of the perennial apricot leaves are not destroyed, dried by a natural drying method, for example, in a shade field, It is sufficient if the useful components of the leaf are crushed to such an extent that it can be extracted sufficiently. In addition, the drying and crushing processes may be carried out in reverse order or repeatedly, if necessary, and may be omitted.

If the content of the extract of the abovementioned timber is less than 0.1% by weight, the effect thereof is insignificant. If the content of the extract is too much, the increase in the effect of the content of the extract is insignificant and the stability of the formulation can not be secured. By weight based on the total weight of the composition.

In addition, the cosmetic composition of the present invention may further comprise 0.1 to 5% by weight of a Kalamanshi extract and 0.1 to 5% by weight of a cacao bean extract.

The further inclusion of the above-mentioned Kalamanshi extract and cacao bean extract gives a superior antioxidative and whitening effect.

The Calamansi extract is prepared by hot water extraction, cold extraction, warming extraction, reflux cooling extraction and ultrasonic extraction using water, ethanol or a mixed solvent thereof in the same manner as in the extraction of the above-mentioned equine tea extract .

At this time, the dry calamans are dried in a known manner to the extent that the useful components of calamans are not destroyed, dried by, for example, natural drying in a shade, Are sufficiently shredded so that they can be extracted sufficiently. In addition, the drying and crushing processes may be carried out in reverse order or repeatedly, if necessary, and may be omitted. In addition, the dry calamans may be used by purchasing commercial products.

When the content is less than 0.1% by weight, the effect thereof is insignificant. When the content is excessive, the increase in the effect of the extract is insignificant, and the stability of the formulation Can not be obtained. Therefore, it is preferable that the amount of the surfactant is in the range of 0.1 to 5% by weight based on the total amount of the composition.

The cacao bean extract is obtained by subjecting cacao bean powder to hot water extraction, cold extraction, warming extraction, reflux cooling extraction, and ultrasonic extraction using water, ethanol or a mixed solvent thereof, in the same manner as in the extraction of the above-mentioned regular-order extract. In this case, the cacao beans are naturally fermented with fleshy seeds and then dried to 5 to 7% moisture, and commercially available products can be purchased and ground to have a particle size of 1 to 5 mm.

The extract of cacao bean exhibits excellent wrinkle improvement and moisturizing effect. If the content is less than 0.1% by weight, the effect thereof is insignificant. If the content is excessive, the increase in the effect of the cacao bean extract is insignificant. , It is preferable that the amount is in the range of 0.1 to 5% by weight based on the total amount of the composition.

In addition, the cosmetic composition of the present invention may contain any conventional ingredients other than the above-mentioned effective ingredients, including conventional additives such as diluents, excipients, buffers, dispersants, surfactants, colorants, perfumes or sweeteners can do. In addition, the cosmetic composition may further include a functional cosmetic ingredient, a moisturizer, and other additives that impart a cosmetic function to the skin, and may further include at least one cosmetically acceptable carrier incorporated in a general skin cosmetic composition . Examples of the carrier component include animal oils, vegetable oils, waxes, paraffin, silicone, lactose, silica, talc, water, ethanol, benzyl alcohol, glycerol aliphatic ester and aliphatic alcohol sulfuric acid. Of course.

In addition, the cosmetic composition of the present invention can be prepared in any formulations conventionally produced in the art, and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, surfactant- But are not limited to, one type of formulation selected from the group consisting of oil, powder foundation, emulsion foundation, wax foundation, spray, and cosmetic pack. More specifically, the present invention relates to a cosmetic lotion composition comprising: a softening lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a cleansing oil, Lip Liner, Brow Pencil, Brow Colorizer, Mascara, Eye Shadow, Eyeliner, Ball Touch, Highlight, Foundation, Base, Powder, Fact, BB Cream, CC Cream, CB Cream, Powder, Shampoo, Manufacture in one formulation of body shampoo, rinse, scalp massager, hair treatment, body lotion, hand wash, body scrub, body oil, shower cologne, detergent, soap, perfume, skin itching relief fluid, skin itching relief cream .

In addition, the formulation examples are the same as those of the conventional cosmetic composition, except that the extract of the present invention is added.

Hereinafter, the present invention will be described in more detail with reference to the following experimental examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example  1: Preparation of equine tea extract

Stems and leaves of Ishikari were collected at a weight ratio of 1: 1, washed thoroughly, and shaken to a moisture content of 15%, which was ground to about 100 mesh. Next, 20 parts by weight of distilled water was added thereto, and the mixture was extracted at 70 to 90 DEG C for 24 hours. The mixture was filtered through a filter paper, concentrated under reduced pressure using a rotary evaporator, and then lyophilized to obtain a powdery extract.

Example  2: Preparation of equine tea extract

Stems and leaves of Ishikari were collected at a weight ratio of 1: 1, washed thoroughly, and shaken to a moisture content of 15%, which was ground to about 100 mesh. Next, 10% by weight of 80% ethanol was added thereto, and the mixture was stirred at 20 캜 for 24 hours for extraction. The mixture was filtered through a filter paper, concentrated under reduced pressure using a rotary evaporator, and then lyophilized to obtain a powdery extract.

Example  3: Preparation of complex extract

The isochrone extract of Example 1 and the equimolar fermentation extract were mixed at a weight ratio of 1: 0.5.

Water and sugar were added to the extract of Example 1 at a weight ratio of 1: 9: 0.1, then Saccharomyces cerevisiae ATCC 18824 was inoculated at 100,000 to 10,000,000 cfu / L, and cultured at 32 ° C for 24 hours And fermented. After the fermentation, the fermentation broth was centrifuged to remove bacteria, ethanol was added thereto to a final concentration of 70% (v / v), refluxed three times for 5 hours, cooled, and filtered. This was concentrated under reduced pressure and lyophilized.

Example 4: Preparation of complex extract

The isochrone extract of Example 2 and the equimolar fermentation extract were mixed at a weight ratio of 1: 0.5.

The fermented extract was prepared by the same method as in Example 3, except that the extract of Example 2 was used.

Example  5: Preparation of complex extract

The extract of Example 3 was mixed with the extract of Wassong, P. forest Leaf, Calamansi and Cacao beans at a weight ratio of 1.5: 0.25: 0.25: 0.25: 0.25.

The wort extract was cut to a size of about 3 cm x 3 cm x 0.5 cm and then shaded to make a water content of 10% or less. About 15 times by weight of water was added thereto, and then heated at 80 to 90 ° C for 15 hours. And extracted. The mixture was filtered, concentrated under reduced pressure using a vacuum concentrator, and lyophilized.

The leaf of Leucospermum japonica L. was shaded with a moisture content of less than 10%, and about 15 times by weight of water was added thereto, followed by hot water extraction at 80 to 90 ° C for 15 hours. The mixture was filtered, concentrated under reduced pressure using a vacuum concentrator, and lyophilized.

About 15 times by weight of water was added to the dried Kallamanshi (commercial name: Hongsilil Company) having a moisture content of 10% or less, and then the Kallamanshi extract was subjected to hot water extraction at a temperature of 80 to 90 ° C for 15 hours. The mixture was filtered, concentrated under reduced pressure using a vacuum concentrator, and lyophilized.

The cacao bean extract was prepared by adding 15 parts by weight of water to cacao beans (seller: Sandare Village) and then subjected to hot water extraction at a temperature of 80 to 90 ° C for 15 hours. The mixture was filtered, concentrated under reduced pressure using a vacuum concentrator, and lyophilized.

Example  6: Preparation of complex extract

The extract of Example 4 and the wanshong extract, acacia leaf extract, Kalamanshi extract and cacao bean extract were mixed at a weight ratio of 1.5: 0.25: 0.25: 0.25: 0.25.

The above extracts of Wakoshi, Liliaceae, Calamansi and Cacao beans were prepared in the same manner as in Example 5.

Experimental Example  1: Antioxidant effect measurement

Free radical scavenging test and an active oxygen scavenging test were conducted to confirm the antioxidative effect of the extracts of Examples 1 to 6 above.

Free radical scavenging test

Using the fact that the absorbance of stable DPPH exhibits the maximum absorbance at 540 nm, it can be said that as the free radical DPPH is erased by the sample and becomes transparent color from purple, that the absorbance decreases at the above 540 nm wavelength as the free radical scavenging rate is increased And proceeded by the following test method.

First, 1 ml of a 0.1 mM DPPH (2,2-diphenyl-1-picryl-hydrazyl radical, Sigma) solution was diluted with a suitable concentration of methanol, and the mixture was incubated at 37 ° C for 15 minutes. Absorbance was measured at a wavelength of 540 nm using a reader (UVT-06685, Thermo max, USA).

In the free radical scavenging test, 1 ml of DPPH and 1 ml of methanol were added to the control, and 1 ml of methanol and 1 ml of the sample were added to obtain the respective color correction values for the sample and the control.

The free radical scavenging ratio was calculated using the following equation (1), and the results are shown in Table 1 below. In Table 1, SC ₅₀ represents the concentration of the sample required to eliminate 50% of the free radicals. The smaller the value, the higher the antioxidant activity.

(1)

Free radical scavenging rate (%) = 100 - {(absorbance of sample / absorbance of control) x 100}

Reactive oxygen scavenging test

The measurement of the absorbance change of nitroblue tetrazolium (NBT) by reactive oxygen species by the enzyme reaction of xanthine / xanthine oxidase (Sigma) It is possible to know the scavenging ability. 0.1 ml of sodium carbonate (Na ₂ CO ₃ ), 0.1 ml of xanthine (Sigma), 0.1 ml of EDTA (ethylenediamine tetraacetic acid), 0.1 ml of BSA (bovine serum albumin, Sigma), 0.1 ml of NBT and 0.1 ml of a sample were placed, (Type 37600 Mixer, Mini mix, USA). The mixture was incubated at 25 ° C for 10 minutes, 0.1 ml of xanthine oxidase was added, and the mixture was incubated at 25 ° C for 20 minutes. ₂ ) was added to stop the reaction, and the absorbance was measured at a wavelength of 540 nm using a microplate reader (UVT-06685, Thermo max, USA).

The control group in the active oxygen scavenging activity test was prepared by adding the third distilled water instead of the sample and measuring by the same method. Third distilled water was added instead of the xanthine oxidase solution to obtain the respective color correction values for the extracted sample and the control group Respectively.

The active oxygen scavenging rate was calculated numerically using the following equation (2), and the results are shown in Table 1. In Table 1, the IC ₅₀ is a sample concentration required to eliminate 50% of active oxygen, and a smaller value means that the antioxidant activity is stronger.

(2)

(%) = 100 - {(absorbance of sample / absorbance of control) x 100}

Results of Experimental Example 1 division Free radical scavenging rate
SC ₅₀ (mg / ml) Active oxygen scavenging rate
IC ₅₀ (mg / ml) Example 1 0.142 0.081 Example 2 0.131 0.072 Example 3 0.113 0.057 Example 4 0.101 0.053 Example 5 0.095 0.052 Example 6 0.089 0.048

As can be seen from the above Table 1, the extract of the present invention showed excellent antioxidative effect.

Experimental Example  2: Antimicrobial activity measurement

To confirm the antimicrobial effect of the extracts of Examples 1 to 6, an antibacterial test was conducted by a paper disk method. Staphylococcus aureus KCTC 6910, Gram-negative bacteria Pseudomonas aeruginosa KCTC 1637, E. coli KCTC 1039, Candida albicans KCTC 7965 and yeast were used as test strains. A total of five strains of Aspergillus niger KCTC 6910 were used.

One liter of each strain cultivated on a plate medium was inoculated into 10 ml of the liquid medium and incubated for 6 hours. After incubation for 6 hours, the bacterial cells per plate medium 10 < ⁷ > CFU / ml and homogenously smoothed with a sterilized smear bar. Then, the sterilized paper disk (6 mm, Satorius, Germany) was placed on a solid plate medium, and the sample dissolved in the solvent was absorbed to be 0.05 ml / disk and cultured. Staphylococcus aureus, Phseudomonas aeruginosa, Escherichia coli were cultured at 37 ° C, Candida yeasts (Chandida albicans) and Aspergillus niger (fungi) were cultured at 27 ° C for 24 hours and 120 hours respectively And the diameter of the transparent zone around the disk was measured. As a positive control, methylparaben, which is known to have a strong antibacterial activity, was used, and the results are shown in Table 2. At this time, the transparent zone around the disc is an indicator of the degree of inhibition of growth of the strain, and the larger the diameter, the higher the antibacterial effect against the strain.

Experimental Example 2 Results Strain Diameter (mm) Staphylococcus
Example 1 13 Example 2 14 Example 3 17 Example 4 18 Example 5 17 Example 6 18 Methyl paraben 11 P. aeruginosa
Example 1 13 Example 2 15 Example 3 15 Example 4 16 Example 5 15 Example 6 16 Methyl paraben 12 Escherichia coli
Example 1 19 Example 2 21 Example 3 22 Example 4 24 Example 5 22 Example 6 24 Methyl paraben 18 Candida yeast
Example 1 14 Example 2 15 Example 3 16 Example 4 17 Example 5 16 Example 6 17 Methyl paraben 13 Black germ
Example 1 26 Example 2 27 Example 3 29 Example 4 30 Example 5 29 Example 6 30 Methyl paraben 24

As can be seen from the above Table 2, the extract according to the present invention showed stronger antibacterial activity than methylparaben, which is known as a strong antibacterial agent.

Experimental Example  3: Tyrosinase  Active inhibition measure

In order to test the whitening effect of the extract according to the present invention, the inhibition of the function of the enzyme called tyrosinase was evaluated. Tyrosinase is an enzyme that helps the production of melanin by stimulating the oxidation process of tyrosine in vivo. Melanin is a black polymer, which melanin is formed in the body by oxidation as described above. When melanin is formed in the upper part of the skin, the skin turns black and produces spots and freckles. The test method is as follows.

0.9 ml of a sample, 1.0 ml of 0.1 M phosphate buffer (pH 6.8) and 1.0 ml of a 1.5 mm L-tyrosine solution were added, and the mixture was maintained at 37 캜 for 10 minutes. After incubation at 37 ° C for 10 minutes, the absorbance was measured at 475 nm using a UV-vis spectrophotometer (Smartspec Plus, Biorad, USA) to determine the inhibition rate against tyrosinase Respectively.

As a control group for the tyrosinase activity inhibition test, a buffer solution was added in place of the sample, and the same colorimetric values were obtained by adding the buffer solution instead of tyrosinase to each of the samples and the control group. As a positive control used to determine the effect, arbutin (Arbutinsynthetic, Sigma), which is known to have excellent whitening effect, was used in several studies. The tyrosinase inhibition rate was numerically calculated using the following equation (3), and the results are shown in Table 3 below. In Table 3 below, IC ₅₀ is the concentration of the sample required to inhibit tyrosinase activity by 50%, and the lower the value, the higher the inhibition rate.

(3)

Tyrosinase inhibition rate (%) = 100 - {(absorbance of sample / absorbance of control group) x 100}

Experimental Example 3 Results division Tyrosinase inhibition rate (IC ₅₀ , ug / ml) Example 1 38 Example 2 35 Example 3 30 Example 4 30 Example 5 28 Example 6 27 Arbutin 30

As can be seen in the above Table 3, the extract according to the present invention showed a whitening effect almost similar to arbutin, which is known as a powerful whitening agent.

Experimental Example  4: Ella Stays  Active inhibition measure

The elastase inhibition activity test was conducted to test the skin wrinkle inhibition and improving effect of the extract according to the present invention. Elastin is a constituent of the matrix layer in the dermis of the skin. As the skin ages, elastin breaks down and the matrix layer in the dermis breaks down and wrinkles occur. (Ala) 3 p-nitroaniline (N-succinyl- (Ala)), which is an elastase substrate, is used as a method for measuring the activity of Elastase, an enzyme that decomposes elastin, 3 p-nitroaniline, Sigma) to determine the change in color caused by the decomposition of p-nitroaniline, by measuring the absorbance at a wavelength of 405 nm. The buffer solution was pH 8.0, 0.267 M Trizma-HCl (Sigma), the substrate solution was 8.8 mm N-succinyl- (Ala) 3 p-nitroaniline and the enzyme solution was 10 ug / ml (Elastase, Sigma, USA ). ≪ / RTI > 20 μl of the buffer solution and 100 μl of the sample solution diluted in the third distilled water for each concentration were mixed with each other. Then, 20 μl of the enzyme solution was added and reacted in a constant temperature water bath at 25 ° C for 15 minutes to obtain the amount of p- (UVT-06685, Thermo max, USA) at a wavelength of 405 nm. At this time, the control group for measuring the activity of the enzyme was measured by the same method with the third distilled water instead of the sample, and the third corrected distilled water was added instead of the enzyme solution to set the case of obtaining the color correction value for each. PMSF (phenylmethylsulfonyl fluoride; Sigma, USA), which is a specific inhibitor of Elastase, was set as a control group in order to determine the effect of inhibiting the Elastase activity of the sample used in the test, and the Elastase inhibition rate And the results are shown in Table 4. < tb >< TABLE > In Table 4 below, the IC ₅₀ is the concentration of the sample required to inhibit the enzyme activity by 50%, and the lower the value, the higher the inhibition rate.

(4)

Elastase activity inhibition rate (%) = 100 - {(absorbance of sample / absorbance of control group) x 100}

Experimental Example 4 Results division Elastase activity inhibition rate (IC50, mg / ml) Example 1 3.14 Example 2 3.10 Example 3 2.95 Example 4 2.88 Example 5 2.79 Example 6 2.69 PMSF 0.07

As can be seen in Table 4, the extract of the present invention showed excellent wrinkle-reducing effect.

Manufacturing example  1 to 4: Production of softening longevity

The softening longevity containing Examples 1 to 4 of the present invention was prepared in a conventional manner at the following proportions.

Component Ratio (% by weight) ingredient Production Example 1 Production Example 2 Production Example 3 Production Example 4 Example 1 5 - - - Example 2 - 5 - - Example 3 - - 5 - Example 4 - - - 5 glycerin 5 5 5 5 1,3-butylene glycol 3 3 3 3 REG1500 One One One One Allantoin 0.1 0.1 0.1 0.1 DL-Panthenol 0.3 0.3 0.3 0.3 Sodium hyaruronate 5 5 5 5 ethanol 10 10 10 10 Oxydodeces-16 0.2 0.2 0.2 0.2 Solisolbait 20 0.2 0.2 0.2 0.2 Unicide Yu 13 0.01 0.01 0.01 0.01 Distilled water Balance Balance Balance Balance

Manufacturing example  5 to 8: Preparation of cream

Creams containing Examples 1-4 of the present invention were prepared in a conventional manner at the following proportions.

Component Ratio (% by weight) ingredient Production Example 5 Production Example 6 Production Example 7 Production Example 8 Example 1 10 - - - Example 2 - 10 - - Example 3 - - 10 - Example 4 - - - 10 Chin type monostearate
glycerin 1.5 1.5 1.5 1.5 Three themal alcohol 1.5 1.5 1.5 1.5 Stearic acid One One One One Polysorbate 60 4.5 4.5 4.5 4.5 Sorbitastarate 0.6 0.6 0.6 0.6 Isostearyl isostere
It 5 5 5 5 Squalane 5 5 5 5 Mineral oil 36 36 36 36 Dimethicone 0.5 0.5 0.5 0.5 Hydroxyethylcellulose 0.12 0.12 0.12 0.12 glycerin 6.0 6.0 6.0 6.0 Trimethylamine 0.7 0.7 0.7 0.7 Unicide Yu 13 0.02 0.02 0.02 0.02 Distilled water Balance Balance Balance Balance

Experimental Example  5: Stability of formulation

For the formulation stability test of the cosmetic composition of the present invention, the cosmetic composition formulated in the above Preparation Examples was placed in an opaque glazed container and kept for 12 weeks in a constant temperature bath kept at 45 DEG C, The degree of discoloration was measured after keeping for 12 weeks in a constantly kept, fully shaded refrigerator. The degree of discoloration of the product is classified into the following six grades (0: no change, 1: very little separation (discoloration), 2: little separation (discoloration), 3: (Discoloration)).

As a result, it was confirmed that all of the compositions of Production Examples were grade 0, in which discoloration and separation did not occur.

Experimental Example  6: Moisturizing, elasticity improvement, antibacterial (acne relief), whitening, pore shrinkage evaluation

The above-mentioned Production Examples 1 to 8 were applied to only the left side of the face for 12 weeks, and then moisturizing, elasticity improvement, antibacterial (acne, , Pore shrinkage effect, and overall satisfaction were evaluated on the 9-point scale. (The right face is used as it is.) (It is evaluated as 4.5 points for the moisturization, elasticity, anti-inflammation, whitening, and pore shrinkage satisfaction on the right face.

Experimental Example 6 Results division Moisturizing elasticity Antibacterial Whitening Pore shrinkage satisfaction Production Example 1 5.6 5.8 7.0 5.6 6.2 6.5 Production Example 2 6.0 6.0 7.2 6.0 6.5 6.8 Production Example 3 6.1 6.2 7.5 6.3 6.7 7.0 Production Example 4 6.3 6.5 7.6 6.5 6.9 7.1 Production Example 5 7.1 7.1 8.5 7.2 7.1 7.5 Production Example 6 7.5 7.3 8.9 7.5 7.5 7.8 Production Example 7 7.6 7.5 8.9 7.8 7.6 8.0 Production Example 8 7.8 7.6 8.9 7.9 7.9 8.2

As can be seen in Table 7, all of the preparations were found to have high satisfaction with moisturizing, elasticity, anti-inflammation, whitening, and pore contraction. In addition, it was confirmed that the evaluation of the cream type in which the content of the extract was higher than the skin type was higher.

Experimental Example  7: Wrinkle improvement effect evaluation

The above-mentioned Production Examples 5 to 8 were applied to 20 womens (groups of 5 or more) in their 30s or older who are undergoing skin aging for 12 weeks for wrinkles on one side of the eye, and then replica of the eye wrinkles was imaged by image analysis The depth (μm) of the wrinkles was measured using a skin wrinkle measuring device (SV-600, C + K, Germany). The other side of the eye used the cosmetics that were used in the past.

As a result, after 12 weeks, the wrinkle depth reduction ratio of Production Example 5 was 4.0%, Production Example 6 was 5.1%, Production Example 7 was 5.5%, and Production Example 8 was 5.8%.

As can be seen from the above results, it was confirmed that the cosmetic composition of the present invention was effective in improving wrinkles.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the scope of the present invention is not limited to the disclosed exemplary embodiments. It will also be appreciated that many modifications and variations will be apparent to those skilled in the art without departing from the scope of the present invention.

Claims (4)

With respect to the total composition weight,
0.1 to 5% by weight of an extract of Orthodox tea extract, 0.1 to 5% by weight of an extract of Fermented tea extract, 0.1 to 5% 5% by weight as an active ingredient,
The fermented extract of Saccharomyces cerevisiae (ATCC 18824), which is a fermented microorganism, is added to the extract of the orderly fermentation extract at a weight ratio of 1: 1 to 10: 0.1-0.2 to 100,000-10,000,000 cfu / L And then fermented at 20 to 40 DEG C for 12 to 60 hours. The cosmetic composition for improving whitening and wrinkle according to claim 1,
delete delete The method according to claim 1,
The composition may further comprise one or more ingredients selected from the group consisting of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a cleansing oil, a powder cleansing, a cosmetic pack, a face scrub, BB cream, CC cream, CB cream, Powder, Shampoo, Body shampoo, Body lotion, Lip liner, Lip liner, Brow pencil, Brow roller, Mascara, Eye shadow, Eyeliner, Ball touch, Highlight, Foundation, Which is one kind of formulation selected from rinses, scalp massagers, hair treatments, body lotions, handwashes, body scrubs, body oils, shower cologins, body cleansers, soaps, perfumes, skin itching relieving liquids and skin itching relief creams Wherein the cosmetic composition is a cosmetic composition for improving whitening and wrinkling.