KR20200056603A - Cosmetic composition including as effective ingredient an extract of citrusfortunella microcarpa - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a calamansi extract as an active ingredient. The cosmetic composition according to the present invention contains the calamansi extract as an active ingredient, wherein calamansi is extracted with water or an aqueous ethanol solution after drying or freezing calamansi in order to maximize the antioxidant efficacy of the calamansi, thereby being usefully applied to cosmetic products with excellent anti-inflammatory effects and skin whitening effects while antioxidant efficacy is maximized.

Description

Cosmetic composition containing Calamanci extract as an active ingredient {COSMETIC COMPOSITION INCLUDING AS EFFECTIVE INGREDIENT AN EXTRACT OF CITRUSFORTUNELLA MICROCARPA}

The present invention relates to a cosmetic composition comprising a callamansi extract as an active ingredient.

Calamansi ((Citrusfortunella microcarpa), belonging to the dicotyledonous tree family, is a fruit native to Southeast Asia. In Calamansi, it contains more vitamin C than lemon, so it has an excellent antioxidant effect, and nobiletin ), Hesperidin (hesperidin) and other ingredients are included to help the liver recovery while reducing the fatigue of the body in addition to the effects of dietary helplessness and dizziness, etc., it is known to have an effective function to burn body fat .

Recently, as the efficacy of such calamansi antioxidant and diet is known, further studies on it have been actively conducted.

On the other hand, as the quality of life of modern people has improved and the interest in people's appearance has increased, the demand for cosmetics has skyrocketed, and among them, interest in functional cosmetics with excellent efficacy such as antioxidant, anti-inflammatory and skin whitening is concentrated. have.

Therefore, among the efficacy of Calamansi, a lot of research has been conducted in the field of cosmetics in relation to the antioxidant effect.

Patent Document 1 proposes a technique for confirming the efficacy associated with the skin, including an extract for calamanci.

More specifically, the technique disclosed in Patent Document 1, based on the total weight of the total composition, 0.1% to 10% by weight of the differential extract, 0.1% to 5% by weight of the differential fermentation extract, 0.1% to 5% by weight of the wasola extract, Contains 0.1% to 5% by weight of the extract of the evergreen leaves, 0.1% to 5% by weight of the Kalamansi extract, and 0.1% to 5% by weight of the cacao bean extract as an active ingredient. After adding water and sugar in a weight ratio of 1: 1 to 10: 0.1 to 0.2, and inoculating fermenting microorganism Saccharomyces cerevisiae ATCC 18824 in an amount of 100,000 cfu / L to 10,000,000 cfu / L It discloses a cosmetic composition for whitening and wrinkle improvement using a differential, characterized in that it is to be fermented for 12 to 60 hours at 20 ℃ to 40 ℃.

However, in Patent Literature 1, rather than maximizing the unique efficacy of Calamansi, it is introduced as a synergistic component for the antioxidant efficacy of the tea extract, and the original effect of Calamansi is not sufficiently exhibited, so there is a problem in that the antioxidant effect is insufficient.

Moreover, the differential extract in Patent Document 1 is a fermented extract that can be produced through a process of fermenting the fermenting microorganism Saccharomyces cerevisiae ATCC 18824, which is time or cost It has a problem that is difficult to mass-produce from the side.

Patent Document 1: Korean Registered Patent Publication No. 10-1875188

The present invention is to solve the problems of the prior art, and is intended to provide an excellent anti-inflammatory and skin whitening effect cosmetic composition while exerting the anti-oxidative effect of Calamanci.

The present invention, in order to solve the above-mentioned problems, provides a cosmetic composition comprising a callamansi extract as an active ingredient.

More specifically, the present invention includes a callamansi (Citrusfortunella microcarpa) extract as an active ingredient, and the callamansi (Citrusfortunella microcarpa) extract is dried or frozen for a 24 hour time as an extraction solution. It provides a cosmetic composition characterized in that it was extracted twice during.

In addition, the present invention, the cosmetic composition provides a cosmetic composition characterized in that it comprises a 0.01% to 20% by weight of the Kalamansi extract relative to the total weight of the cosmetic composition.

In addition, the present invention provides the cosmetic composition, characterized in that the extraction solution is water or an aqueous ethanol solution.

In addition, the present invention, the ethanol aqueous solution provides a cosmetic composition characterized in that the ethanol 30% to 99% aqueous solution.

In addition, the present invention provides the cosmetic composition, wherein the cosmetic composition is used for anti-aging or anti-inflammatory use.

In addition, the present invention, the cosmetic composition provides a cosmetic composition characterized in that it is used for skin whitening purposes.

In addition, the present invention, the cosmetic composition is a lotion, skin lotion, moisture lotion, nutrition lotion, skin toner, essence, foundation, nutrition cream, hand cream, moisture cream, massage cream, mask pack, all-in-one cream, BB cream, CC It provides a cosmetic composition characterized in that it is formulated with at least one selected from the group consisting of cream, soap, body lotion, body cream, body cleanser, cleansing lotion, cleansing foam, shampoo, conditioner or conditioner.

In addition, the present invention, the cosmetic composition is a fat substance, an organic solvent, a solubilizer, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizing agent, a foaming agent, a fragrance, a surfactant, water, and an ionic type Or further comprising at least one adjuvant selected from nonionic emulsifiers, fillers, metal ion blockers and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles It provides a cosmetic composition characterized in that.

The cosmetic composition according to the present invention contains the extract of Calamansi as an active ingredient, and dried or frozen the Calamansi to extract the water or ethanol solution to maximize the antioxidant efficacy of the Calamansi, In addition to maximizing the antioxidant efficacy, the anti-inflammatory effect and the skin whitening effect can be usefully applied to excellent cosmetics at the same time.

The accompanying drawings are for explaining the contents of the present invention in more detail to those skilled in the art, and the technical spirit of the present invention is not limited thereto.
1 is a graph showing the results of measuring the total phenol content in Experimental Example 1 according to the present invention as a graph.
Figure 2 is a graph showing the results of the DPPH radical scavenging activity of the Calamansi juice in Experimental Example 2 according to the present invention.
3 is a graph showing the results of DPPH radical scavenging activity of water and ethanol extracts of frozen or dried calamansi in Experimental Example 2 according to the present invention.
4 is a graph showing a result of measuring the nitrite scavenging ability of the calamansi juice in Experimental Example 3 according to the present invention as a graph.
5 is a graph showing the results of measuring nitrite scavenging activity of water and ethanol extracts of frozen or dried calamansi in Experimental Example 3 according to the present invention.
6 is a graph showing the results of measuring the superoxide radical scavenging activity of the calamansi juice in Experimental Example 4 according to the present invention.
7 is a graph showing the results of superoxide radical scavenging activity of water and ethanol extracts of frozen or dried calabash in Experimental Example 4 according to the present invention.
8 is a graph showing the results of cell viability measurement (cytotoxicity evaluation) of frozen and dried calamansi water and ethanol extract in Experimental Example 5 according to the present invention.
9 is a graph showing the results of the evaluation of the inhibition of nitrogen monoxide (NO) production (anti-inflammatory effect evaluation) of water and ethanol extracts of frozen or dried calamansi in Experimental Example 6 according to the present invention.
10 is a graph showing the results of L-DOPA oxidation inhibition (evaluation of skin whitening effect) of water and ethanol extracts of frozen or dried calamansi in Experimental Example 7 according to the present invention.

Hereinafter, the cosmetic composition according to the present invention will be described in detail, but the scope of the cosmetic composition is not limited by the following description.

The present invention relates to a cosmetic composition.

More specifically, the present invention relates to a cosmetic composition comprising Calamansi (Citrusfortunella microcarpa) extract as an active ingredient.

The Kalamansi (Citrusfortunella microcarpa) extract is not particularly limited, but is preferably dried or frozen Kalamansi.

If the kalamanshi is used as it is without a separate drying or freezing process, it may be difficult to extract the components necessary for exerting efficacy according to the present invention in the kalamanshi extraction process.

The drying process is not particularly limited, but, for example, by purchasing a Calamansi (Citrusfortunella microcarpa), and then removing the skin, washed with purified water, it can be carried out through a process of complete drying at 60 ℃.

In addition, the freezing process is not particularly limited, but, for example, by purchasing the Calamansi (Citrusfortunella microcarpa), and then removing the shell, washed with purified water, and then frozen at -10 ℃ can be performed through the process of freezing. have.

The kalamansi extract may be extracted twice for 24 hours as an extraction solution, and in this case, it is preferable to use an aqueous solution of water or ethanol.

When using an ethanol aqueous solution as the extraction solution, it may be 30% to 99%, 40% to 80%, 50% to 80% or 60% to 80%, and preferably 70% ethanol aqueous solution is used. Do.

When the extraction process and the extraction solution are made or used differently from the above, it may not be able to function as an original function due to its own change in the components of Calamansi, and may not be extracted as an extract.

The cosmetic composition may include the Kalamansi extract in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition.

In this case, the content range of the Kalamansi extract may be, for example, 0.01% to 20% by weight, 0.1% to 15% by weight, or 1% to 10% by weight, and When included, the efficacy of the extract is insufficient, or the viscosity of the liquid increases during the extraction process, which may cause problems when applied as a finished product formulation, such as a problem in filtration.

As described above, the cosmetic composition according to the present invention can be used for anti-aging or anti-inflammatory purposes at the same time as acting as an antioxidant by including the Kalamansi extract as an active ingredient.

Specific experiments and experimental results for use in the anti-aging or anti-inflammatory use were described in experimental examples described later.

In addition, the cosmetic composition according to the present invention can be used for skin whitening purposes.

Likewise, specific experiments and experimental results for use in the skin whitening application were described in experimental examples described later.

The appearance of the cosmetic composition contains a cosmetic or dermatologically acceptable medium or base. These are all formulations suitable for topical application, e.g. solutions, gels, solids, dough anhydrous products, emulsions, suspensions, microemulsions, microcapsules, microgranules, or ionic (liposomes) obtained by dispersing an oil phase in an aqueous phase, and It can be provided in the form of a non-ionic vesicle dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. Therefore, the cosmetic composition is a lotion, skin lotion, moisture lotion, nutrition lotion, skin toner, essence, foundation, nutrition cream, hand cream, moisture cream, massage cream, mask pack, all-in-one cream, BB cream, sea cream, soap, It may be formulated as one or more selected from the group consisting of body lotion, body cream, body cleanser, cleansing lotion, cleansing foam, shampoo, conditioner or conditioner.

In addition, these compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, as a formulation that can be applied to the skin, it can be prepared and used as an absorbent gel, pad, patch, sponge, and mask pack composition, but is not limited thereto.

In addition, the cosmetic composition is a fat substance, organic solvent, solubilizer, thickening agent and gelling agent, softening agent, antioxidant, suspending agent, stabilizer, foaming agent, fragrance, surfactant, water, ionic or nonionic type Emulsifiers, fillers, metal ion blockers and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles may further include one or more adjuvants.

In addition, the cosmetic composition according to the present invention is not particularly limited in the form to be applied, for example, may be applied in a form applied to the skin, or may be applied in a form absorbed into the skin using a microneedle or the like.

Hereinafter, the present invention will be described in more detail through specific experimental examples. However, these experimental examples are for illustrative purposes only, and the scope of the present invention is not limited to these experimental examples.

[Production Example] Preparation of Calamanci Extract

The Kalamanshi used in this example was purchased and sold after being frozen and dried without using it. Frozen or dried calabash was purchased and used commercially available frozen or dried calabash.

10 times (frozen) or dried (50 times) water or 70% ethanol was added to 100 g of the frozen or dried calamanshi, and extracted twice for 24 hours, and then the extract was filtered with filter paper. The filtrate was removed from water or ethanol using a rotary vacuum evaporator (EYELA A-1000S, Tokyo Rikakikai Co., Tokyo, Japan) on a 37-40 ° C. water bath, and freeze-dried to prepare a Kalamansi extract. It was used as.

The extraction yield for each extraction solvent for each sample of Calamanci is shown in [Table 1] below.

sample extract yield(%) Dry calabash Water extract 42 70% ethanol extract 50 Frozen Calamansi Water extract 8 70% ethanol extract 10

[Experimental Example 1] Total phenol content measurement

The content of the phenolic compound was measured using the Folin-Denis method using the principle of showing blue color by reacting with the phenolic substance phosphomolybdic acid. 50 μL of sample solution at a concentration of 0.5 mg / mL and 50 μL of Folin-Ciocalteu's phenol reagent (Sigma-Aldrich Co., St. Louis, MO, USA) were mixed for 3 minutes at room temperature, followed by dark reaction, followed by 10% sodium carbonate (Na ₂ CO ₃ , Duksan Pure Chemical Co., Ltd.) solution was added, 750 μL of solution was left in the dark for 1 hour, and the absorbance of the supernatant was measured at 765 nm. At this time, the content of the phenolic compound was calculated using mg gallic acid per 1 g of the sample after preparing a standard calibration curve using gallic acid (Sigma-Aldrich Co.) as a standard.

The results of measuring the total phenol content are shown in [Table 2] below, and are shown in FIG. 1 as a graph.

division Total phenol contents (GAE mg / g DW) Calamansi Juice 11.1 ± 1.81 Frozen Calamansi Water extract 29.55 ± 4.33 Ethanol extract 15.33 ± 2.70 Dry calabash Water extract 39.30 ± 6.36 Ethanol extract 28.10 ± 3.55

[Experiment 2] DPPH radical scavenging activity

DPPH radical (1,1-diphenyl-2-picrylhydrazyl) scavenging activity was evaluated using the Blois method. 0.2mM DPPH solution was added 1: 1 to the sample prepared at a certain concentration, followed by vortex mixing and dark reaction at room temperature for 30 minutes, and then measuring the absorbance at 517 nm using a spectrophotometer to determine the decrease in absorbance by reduction of DPPH. At this time, the IC50 value (mg / mL) represents the concentration of the sample that reduces the value of the control group containing the solvent used to dissolve the sample instead of the sample by 50%, and is compared using ascorbic acid (Sigma-Aldrich Co.) as a positive control. Did. DPPH radical scavenging ability was calculated by the following equation.

DPPH scavenging activity (%) = {1- (absorbance of sample / absorbance of control)} × 100

The results of DPPH radical scavenging activity of the calamansi juice are shown in [Table 3] below, and shown in FIG. 2 as a graph.

density(%) Inhibition rate (mean ± standard deviation) 0.025 5 ± 5.1 0.05 12 ± 0.6 0.1 34 ± 0.8 0.5 68 ± 1.3 One 96 ± 1.4 2.5 101 ± 2.2 ※ unit : %

In addition, the results of DPPH radical scavenging activity (inhibition rate) of the frozen or dried calamansi water and ethanol extracts are shown in [Table 4] below, and shown in FIG. 3 as a graph.

density
(mg / mL) Frozen Calamansi Dry calabash Water extract 70% ethanol extract Water extract 70% ethanol extract 0.05 14.26 ± 0.79 46.18 ± 0.39 16.75 ± 1.44 16.75 ± 1.55 0.125 16.16 ± 2.57 51.55 ± 0.15 15.35 ± 0.92 15.35 ± 0.44 0.25 31.93 ± 1.66 59.83 ± 2.23 32.33 ± 0.39 32.33 ± 0.87 0.5 45.80 ± 0.22 83.83 ± 7.74 41.60 ± 4.42 41.60 ± 2.32 One 60.49 ± 0.30 88.12 ± 5.49 59.81 ± 1.21 57.53 ± 1.86 2.5 104.16 ± 1.49 100.97 ± 1.26 114.44 ± 8.40 114.44 ± 0.48 5 148.29 ± 2.58 105.76 ± 1.11 179.05 ± 27.43 179.05 ± 0.58 ※ unit : %

[Experimental Example 3] Measurement of nitrite scavenging activity

The nitrite scavenging ability was measured by Gray and Dugan's method. After adding 2.5, 5, and 10 mg / mL of each extract to 1 mL of 1 mM NaNO ₂ solution, adjust the pH of the reaction solution to 1.2 with 0.1N HCl (pH 1.2), make the total amount to 10 mL, and react at 37 ° C for 1 hour. Ordered. Griess × reagent (1% sulfanilic acid: 1% naphthylamine = 1: 1) was prepared with 5 mL of 2% acetic acid solution and 30% acetic acid. 0.4 mL was added to 1 mL of each reaction solution to which the prepared Griess reagent was reacted and mixed well. After the mixture was left at room temperature for 15 minutes, the absorbance was measured at 520 nm to measure the amount of nitrite remaining. The blank test was conducted in the same manner by adding 0.4 mL of distilled water instead of the Griess reagent. The nitrite scavenging action was expressed as a percentage when the sample was added and when the sample was not added.

The results of measuring the nitrite scavenging ability of the calamansi juice are shown in [Table 5] below, and shown in FIG. 4 as a graph.

density(%) Inhibition rate (mean ± standard deviation) 0.5 4.84 ± 0.88 One 8.41 ± 0.53 2.5 18.65 ± 1.61 5 31.80 ± 0.90 10 49.03 ± 0.04 20 94.59 ± 0.01 ※ unit : %

In addition, the results of measuring nitrite scavenging activity (lower rate) of the frozen or dried calamansi water and ethanol extracts are shown in [Table 6] below, and shown in FIG. 5 as a graph.

density
(mg / mL) Frozen Calamansi Dry calabash Water extract 70% ethanol extract Water extract 70% ethanol extract 0.25 - 5.07 ± 0.42 - 7.33 ± 1.70 0.5 0.00 ± 0.98 4.54 ± 0.64 14.63 ± 0.38 12.17 ± 0.34 One 1.94 ± 0.63 9.57 ± 0.26 17.09 ± 0.26 15.82 ± 0.23 2.5 8.54 ± 0.42 24.65 ± 0.87 24.46 ± 0.08 26.77 ± 0.11 5 20.72 ± 1.32 33.27 ± 0.64 36.60 ± 1.02 40.00 ± 0.11 12.5 33.27 ± 0.64 62.34 ± 0.08 65.10 ± 0.42 70.81 ± 0.76 25 66.69 ± 0.00 85.48 ± 0.30 89.68 ± 1.93 92.33 ± 0.34 ※ unit : %

[Experimental Example 4] Measurement of superoxide radical scavenging activity

In general, superoxide radicals are generated by xanthine oxidase in vitro. Superoxide radical scavenging activity was measured using the NBT (nitro-blue tetrazolium) reduction method (Parejo et al., 2002). That is, 0.5 mL of 0.1 mM phosphate buffer (pH 8.0) containing 0.8 mM xanthine and 0.1 mM phosphate buffer (pH 8.0) containing 0.48 mM NBT were treated with extracts at respective concentrations to make a mixture, and the mixture was 37 ° C. After reacting for 20 minutes, 2.0 mL of 69 mM SDS was added to terminate the reaction, and absorbance was measured at 560 nm. The concentration of the extract that reduces the inhibitory ability against NBT reduction by 50% according to the amount of sample added by concentration (IC50, 50% inhibition concentration) is shown.

The results of measuring the superoxide radical scavenging ability of the calamansi juice are shown in [Table 7] below, and shown in FIG. 6 as a graph.

density(%) Erasing capacity (mean ± standard deviation) 0.01 7.73 ± 0.51 0.05 14.17 ± 2.65 0.1 20.42 ± 0.11 0.5 50.70 ± 0.60 One 74.93 ± 0.62 5 85.63 ± 1.37 10 104.00 ± 0.17 ※ unit : %

In addition, the results of measuring nitrite scavenging ability (lower rate) of the frozen or dried calamansi water and ethanol extracts are shown in [Table 8] below, and shown in FIG. 7 as a graph.

density
(mg / mL) Frozen Calamansi Dry calabash Water extract 70% ethanol extract Water extract 70% ethanol extract 0.5 - 13.42 ± 0.35 4.90 ± 1.29 2.94 ± 0.55 One 2.13 ± 0.65 16.39 ± 0.47 8.86 ± 0.96 6.34 ± 0.61 2.5 12.83 ± 0.45 22.05 ± 1.14 20.73 ± 0.05 8.70 ± 0.49 5 30.23 ± 0.28 34.64 ± 0.66 40.81 ± 0.13 12.64 ± 0.29 12.5 63.77 ± 0.61 48.37 ± 1.98 72.96 ± 0.3 21.60 ± 0.80 25 101.96 ± 0.74 80.03 ± 1.27 98.52 ± 1.66 61.81 ± 0.89 50 112.92 ± 2.62 92.14 ± 0.58 90.76 ± 4.32 98.38 ± 0.04 ※ unit : %

[Experimental Example 5] Cell viability measurement (cytotoxicity evaluation)

As an experiment for measuring cell viability, a water soluble tetrazolium salt-1 (WST-1) assay method was used. RAW264.7 cells were dispensed in a 96-well plate at a concentration of 1.5 × 10 ⁵ cell / well, and after 24 hours of culture, the existing medium was removed, and 1 ug / ml LPS and samples (0.1, 0.5, 1, 2.5, 5 mg / ml) Concentration) and cultured for 24 hours by replacing the medium with the same treatment. After cell culture, the WST-1 solution was incubated at 37 ° C for 30 minutes by adding 10% of the medium per well, and the absorbance at 450 nm was measured using a Microplate reader.

The results of cell viability measurements (cytotoxicity evaluation, viability) of the frozen or dried calamanci water and ethanol extracts are shown in [Table 9] below, and shown in FIG. 8 as a graph.

division Concentrate (mg / mL) Experimental group 0.1 0.5 One 2.5 5 DW 94.6 92.4 80.9 54.7 47.7 DE 88.0 86.1 81.0 59.5 46.4 FW 92.4 84.0 80.5 64.1 52.8 FE 88.1 88.2 70.6 51.6 26.8 ※ Control 100 standard
※ Measured value is rounded off to the second decimal place
※ unit : %

[Experimental Example 6] Nitrogen monoxide (NO) production inhibition evaluation (anti-inflammatory effect evaluation)

RAW264.7 cells were dispensed into a 96-well plate at a concentration of 1.5 × 10 ⁵ cell / well to remove the existing medium after 24 hours of incubation, and 0.05 based on 1mg / ml, the final concentration of the sample showing a survival rate of 80% or more. , 0.1, 0.25, 0.5, 1mg / ml was replaced with a medium containing the concentration and cultured for 24 hours. The amount of NO generated was measured in the form of NO ₂ ^- present in the cell culture using a griess reagent. After mixing the cell culture supernatant 100uL and the griess reagent 100uL for 10 minutes at room temperature, the absorbance was measured at 540nm, and the NO concentration of the culture was compared with NaNO ₂ standard.

The results of the evaluation of nitrogen monoxide (NO) production inhibition (NO production) of the frozen or dried calamansi water and ethanol extracts are shown in [Table 10] below, and shown in FIG. 9 as a graph.

division Concentrate (mg / mL) Experimental group 0.05 0.1 0.25 0.5 One DW 25.3 26.3 25.3 20.7 10.7 DE 25.0 27.2 25.9 22.7 15.1 FW 25.1 25.8 24.8 20.8 10.5 FE 24.5 24.9 23.5 21.2 13.0 ※ The measured value is 26.8 when treated with LPS as a control.
※ Measured value is rounded off to the second decimal place
※ Unit: uM

[Experimental Example 7] L-DOPA oxidation inhibitory effect (skin whitening effect evaluation)

0.2 ml of 10 mM L-DOPA and 0.5 ml of 0.175 M sodium phosphate buffer (pH 6.8) and 0.2 ml of mushroom tyrosinase (110 U / ml) were added to a mixture of 0.1 ml of sample solution, and reacted at 37 ° C for 10 minutes to generate DOPA generated in the reaction solution. Chrome was measured at a wavelength of 475 nm. The tyrosinase inhibitory activity was expressed as the rate of decrease in absorbance between the sample solution and the addition solution.

The L-DOPA oxidation inhibitory effect (skin whitening effect evaluation) results of the frozen or dried calamansi water and ethanol extracts are shown in [Table 11] below, and are shown in FIG. 10 as a graph.

division Concentrate (mg / mL) Experimental group 0.1 0.5 One 2 5 DW 0.4 8.4 17.4 45.7 82.8 DE 0 0.4 9.4 38.0 79.8 FW 0 2.0 6.2 36.3 80.5 FE 1.7 7.2 19.3 44.9 84.5 ※ Measured value is rounded off to the second decimal place
※ unit : %

As can be seen from the above [Table 1] to [Table 11] and FIGS. 1 to 10, the cosmetic composition comprising the Calamansi extract according to the present invention as an active ingredient, includes the Calamansi extract as an active ingredient However, in order to maximize the antioxidant efficacy of the calamansi, the calamansi was dried or frozen and extracted with an aqueous or ethanol aqueous solution, and in addition to maximizing the antioxidant efficacy, anti-inflammatory effect and skin whitening effect are also excellent in cosmetics. It can be usefully applied.

The foregoing description of the present application is for illustration, and a person having ordinary knowledge in the technical field to which the present application belongs will understand that it is possible to easily change to other specific forms without changing the technical spirit or essential characteristics of the present application. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present application is indicated by the claims, which will be described later, rather than the detailed description, and all changes or modifications derived from the meaning and scope of the claims and equivalent concepts should be interpreted to be included in the scope of the present application.

Claims (8)

Calamansi (Citrusfortunella microcarpa) extract as an active ingredient,
The Calamansi (Citrusfortunella microcarpa) extract is a cosmetic composition characterized in that the dried or frozen Calamansi (Citrusfortunella microcarpa) is extracted twice with an extraction solution for 24 hours.
According to claim 1, wherein the cosmetic composition is a cosmetic composition characterized in that it comprises a Kalamansi extract in an amount of 0.01 to 20% by weight relative to the total weight of the cosmetic composition.
The cosmetic composition according to claim 1, wherein the extraction solution is an aqueous solution of water or ethanol.
The cosmetic composition according to claim 3, wherein the aqueous ethanol solution is an aqueous 30% to 99% ethanol solution.
The cosmetic composition according to claim 1, wherein the cosmetic composition is used for anti-aging or anti-inflammatory purposes.
The cosmetic composition according to claim 1, wherein the cosmetic composition is used for skin whitening.
The method of claim 1, wherein the cosmetic composition is a lotion, skin lotion, moisture lotion, nutrition lotion, skin toner, essence, foundation, nutrition cream, hand cream, moisture cream, massage cream, mask pack, all-in-one cream, BB cream, CC A cosmetic composition characterized by being formulated with at least one selected from the group consisting of cream, soap, body lotion, body cream, body cleanser, cleansing lotion, cleansing foam, shampoo, conditioner or conditioner.
The method of claim 1, wherein the cosmetic composition is a fat substance, an organic solvent, a solubilizer, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizing agent, a foaming agent, a fragrance, a surfactant, water, and an ionic type Or nonionic emulsifiers, fillers, metal ion blockers and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, one or more adjuvants selected from lipid vesicles Cosmetic composition, characterized in that.

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