KR20180102928A

KR20180102928A - A cosmetic composition for skin antiaging comprising an extract of Citrusfortunella microcarpa as an active ingredient

Info

Publication number: KR20180102928A
Application number: KR1020170029682A
Authority: KR
Inventors: 우의택; 최정환
Original assignee: 우의택; 주식회사 이앤코리아
Priority date: 2017-03-08
Filing date: 2017-03-08
Publication date: 2018-09-18

Abstract

The present invention relates to a cosmetic composition for skin antiaging comprising an extract of Citrofortunella microcarpa as an active ingredient. The extract of Citrofortunella microcarpa of the present invention has excellent skin antiaging and antioxidative effects, and thereby can be applied to a cosmetic product for skin antiaging.

Description

[0001] The present invention relates to an anti-aging cosmetic composition comprising an extract of Kalamansi as an active ingredient,

The present invention relates to an anti-aging cosmetic composition comprising an extract of Citrusfortunella microcarpa as an active ingredient.

As the quality of life of modern people has improved, interest in cosmetics has soared as people's interest in cosmetics has increased. Among them, products with excellent anti-aging effects are attracting attention.

Calamansi (Citrusfortunella microcarpa) is a citrus plant with the oriental cultivated in Southeast Asia such as Vietnam, the Philippines and Malaysia. It is also called Citrus microcarpa bunge, Kalamansi, Calamondin. Calaman City contains more than 20 times more vitamin C than lemon, and it has excellent antioxidative effect. It contains ingredients such as nobiletin and hesperidin to help recovery of the liver and relieve physical fatigue. It has effect. It is also known to have an effective function for alleviating lethargy and dizziness caused by a diet and burning body fat.

As described above, it is known that various functions are contained in the calamans, and a patent related to calamans discloses a method for stabilizing anthocyanin-containing natural extracts using calamans (Patent Document 1).

Accordingly, the inventors of the present invention have developed a cosmetic composition having an excellent anti-aging effect including the Kalamanshi extract by confirming that the anti-aging effect is significantly exerted when the extract of Kalamansi is contained as an active ingredient and contained in an appropriate ingredient ratio, .

Korean Patent No. 10-1543569 Korean Patent Publication No. 10-2016-0131246

It is an object of the present invention to provide an anti-aging cosmetic composition comprising an extract of Citrusfortunella microcarpa as an active ingredient.

The present invention provides an anti-aging cosmetic composition comprising an extract of Citrus fortunella microcarpa as an active ingredient.

The anti-aging cosmetic composition may contain 0.001 to 50% by weight of Kalamanshi extract based on the total weight of the cosmetic composition.

The Calamansi extract may be extracted with one or more solvents selected from the group consisting of water or an aqueous ethanol solution.

The aqueous ethanol solution may be an aqueous solution of 30 to 99% ethanol.

The anti-aging cosmetic composition may be in the form of a lotion, a skin lotion, a moisturizing lotion, a nutrition lotion, a skin toner, an essence, a foundation, a nutrition cream, a hand cream, a moisturizing cream, a massage cream, a mask pack, A body lotion, a body lotion, a body cream, a body cleanser, a cleansing lotion, a cleansing foam, a shampoo, a rinse or a conditioner.

The extract of Kalamansi according to the present invention has excellent antioxidative and anti-aging effects and thus can be applied as an anti-aging cosmetic.

FIG. 1 is a graph showing DPPH radical scavenging activity of extract of Kalamansi according to extraction solvent.
FIG. 2 shows the ABTS radical scavenging activity of the extract of Kalamansi according to the extraction solvent.
FIG. 3 is a graph showing the total polyphenol content of the extract of Kalamansi by the extraction solvent. FIG.
FIG. 4 is a graph showing the total flavonoid content of the extract of Kallamansi according to the extraction solvent. FIG.

The anti-aging cosmetic composition may contain 0.001 to 50% by weight of Kalamanshi extract based on the total weight of the cosmetic composition, but is not limited thereto, preferably 0.1 to 50% by weight.

When the amount of the Kalamanshi extract is less than 0.1% by weight, the anti-aging effect is difficult to be exerted. If it exceeds 10% by weight, large irritation to the skin may occur or the stability of the composition may be deteriorated .

According to a most preferred embodiment of the cosmetic composition for use in the present invention, the amount of Kalamansi extract contained in the composition of the present invention is 5% by weight.

The aqueous ethanol solution may be an aqueous solution of 30 to 99% ethanol.

In preparing the Kalamanshi extract, the Kalamanshi powder may be added in an amount of 10-30% by weight based on the total weight of the solvent.

When the extract is added in an amount of less than 10% by weight, the efficacy of the extract is lowered. When the extract is added in an amount of 30% by weight or more, viscosity of the liquid phase increases, resulting in problems in filtration.

The Kalamanshi powder was washed with purified water after removing the husks obtained in Vietnam and then completely dried at 60 ° C. The dried calamansi were prepared in powder form using a pulverizer.

In one embodiment of the present invention, 10-30% by weight of Kalamansi powder was shaken with distilled water, ethanol 30, 60, 80, 99% aqueous solution, and the filtrate was collected by filtration after 6 hours. The recovered extract was concentrated using a vacuum concentrator, and then subjected to freeze-drying to obtain a final extract.

The anti-aging cosmetic composition containing the extract of Kalamansi as an active ingredient according to the present invention can be used as a cosmetic composition for skin care, which comprises a fat substance, an organic solvent, a solubilizing agent, a thickener, a gelling agent, a softener, an antioxidant, a suspending agent, a stabilizer, A chelating agent, a preservative, a vitamin, a blocking agent, a wetting agent, an essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent, a lipid vesicle or a lipid vesicle, Such as any other ingredients commonly used in cosmetics, or adjuvants commonly used in the field of dermatology. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

The external form of the cosmetic composition contains a cosmetically or dermatologically acceptable medium or base. It may be in any form suitable for topical application, for example, as a solution, a gel, a solid, a paste anhydrous product, an emulsion obtained by dispersing the oil phase in water, a suspension, a microemulsion, a microcapsule, In the form of a non-ionic follicle dispersing agent, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. These compositions may be prepared according to conventional methods in the art. The composition according to the invention may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The anti-aging cosmetic composition containing the extract of Kalamansi as an active ingredient according to the present invention is not particularly limited in its formulation, and examples thereof include lotion, skin lotion, moisturizing lotion, nutrition lotion, skin toner, essence, foundation , Nutrition Cream, Hand Cream, Moisture Cream, Massage Cream, Mask Pack, All-in-One Cream, Bib Cream, Cream Cream, Soap, Body Lotion, Body Cream, Body Cleanser, Cleansing Lotion, Cleansing Foam, Shampoo, Rinse or Conditioner And the like.

In addition, the composition can be used as a formulation for absorbing the skin, but it is not limited to the composition of the absorbent gel, pad, patch, sponge and mask pack.

The cosmetic composition as described above may be applied to the skin, or may be applied to the inside of the skin using a micro needle or the like.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are intended to illustrate the contents of the present invention, but the scope of the present invention is not limited to the following examples. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art.

< Example 1 to 5> Kalamansi Extract preparation

Citrusfortunella microcarpa, native to the Philippines, was purchased, washed with purified water, and completely dried at 60 ° C. The dried Calamansi were crushed using a crusher and refrigerated until they were extracted.

Extraction was carried out under various solvent conditions in order to prepare the optimum extract for use in the experiment using Kalamanshi powder. The extraction solvent was distilled water, ethanol 30, 60, 80, 99%, and 10-30% by weight of Kalamanshi powder was shaken with respect to the total weight of each extraction solvent, and after 6 hours, the extract was collected by filtration. The extracted extract was concentrated by using a vacuum concentrator and then lyophilized to obtain a final extract. The distilled water extract was used in Example 1, the 30% ethanol extract was used in Example 2, the 60% ethanol extract was used in Example 3, the ethanol 80% Example 4, ethanol 99% extract was named as Example 5.

The extracts were stored in the refrigerator until used in the experiment. To minimize the effect on the stability of the physiologically active substance, the effective period of the extract was set to 3 days, .

< Example 6 to 10> Cream Cosmetics Composition manufacturing

The cream cosmetic composition of Examples 6 to 10 and the cream cosmetic composition of Comparative Example 1, to which Examples 1 to 5 were added, respectively, were prepared according to the following Table 1. The phase A of the water phase consisting of the raw materials 1 to 4 and the phase B of the oil phase composed of the raw materials 5 to 13 were uniformly heated to 80 DEG C respectively and then the phase B was slowly added to the phase A and emulsified at 3000 rpm for 7 minutes . The prepared emulsion was charged with C phase as a neutralizing agent composed of 14 to 15 raw materials and neutralized at 3200 rpm for 7 minutes. After the contents were cooled to 40 ° C, the remaining ingredients were added and stirred to prepare the final cream cosmetic composition.

Item Raw material name Example 6 Example 7 Example 8 Example 9 Example 10 Comparative Example 1 A phase

1. Disodium di 0.02 0.02 0.02 0.02 0.02 0.02 2. Methylparaben 0.20 0.20 0.20 0.20 0.20 0.20 3. Carbomer 1% aqueous solution 12.00 12.00 12.00 12.00 12.00 12.00 4. Purified water Balance Balance Balance Balance Balance Balance B phase

5. Tocopheryl acetate 0.10 0.10 0.10 0.10 0.10 0.10 6. Cetearyl alcohol 1.60 1.60 1.60 1.60 1.60 1.60 7. Methylglucose Diolate 0.30 0.30 0.30 0.30 0.30 0.30 8. Pigment-20 Methylglucossexquartearate 1.00 1.00 1.00 1.00 1.00 1.00 9. Polysorbate 60 1.20 1.20 1.20 1.20 1.20 1.20 10. Glyceryl Stearate / Phage-100 Stearate 1.50 1.50 1.50 1.50 1.50 1.50 11. Stearic acid 1.00 1.00 1.00 1.00 1.00 1.00 12. Polydecenes 4.00 4.00 4.00 4.00 4.00 4.00 13. Jojoba oil 5.00 5.00 5.00 5.00 5.00 5.00 C phase
14. Triethanolamine 0.14 0.14 0.14 0.14 0.14 0.14 15. Purified water 2.00 2.00 2.00 2.00 2.00 2.00 D phase

16. Phenoxyethanol 0.50 0.50 0.50 0.50 0.50 0.50 17. Spices a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount 18. Example 1 5 - - - - - 19. Example 2 - 5 - - - - 20. Example 3 - - 5 - - - 21. Example 4 - - - 5 - - 22. Example 5 - - - - 5 -

< Example 11 to 15> Lotion flow Cosmetics Composition manufacturing

The lotion-type cosmetic compositions of Examples 11 to 15 and the lotion-type cosmetic compositions of Comparative Example 2, to which Examples 1 to 5 were respectively added, were prepared according to the following Table 2. The phase A of the water phase consisting of raw materials 1 to 4 and the phase B of the oil phase composed of the raw materials 5 to 14 were uniformly heated to 80 DEG C respectively and then the phase B was slowly fed into the phase A and emulsified for 7 minutes at 3000 rpm . The prepared emulsion was charged with C phase, which is a neutralizing agent composed of 15 to 16 raw materials, and neutralized at 3200 rpm for 7 minutes. After the contents were cooled to 40 캜, the remaining ingredients were added and stirred to prepare a final lotion-type cosmetic composition.

Item Raw material name Example 11 Example 12 Example 13 Example 14 Example 15 Comparative Example 2 A phase

1. Disodium di 0.02 0.02 0.02 0.02 0.02 0.02 2. 2% aqueous solution of acrylate / seed 10-30 alkyl acrylate crosspolymer 5.00 5.00 5.00 5.00 5.00 5.00 3. Carbomer 1% aqueous solution 18.00 18.00 18.00 18.00 18.00 18.00 4. Purified water Balance Balance Balance Balance Balance Balance B phase

5. Methylglucose Diolate 0.40 0.40 0.40 0.40 0.40 0.40 6. Polysorbate 60 1.40 1.40 1.40 1.40 1.40 1.40 7. Chin type glyceryl stearate 0.60 0.60 0.60 0.60 0.60 0.60 8. Cetearyl alcohol 0.80 0.80 0.80 0.80 0.80 0.80 9. Stearic acid 0.30 0.30 0.30 0.30 0.30 0.30 10. Polydecen 8.00 8.00 8.00 8.00 8.00 8.00 11. Jojoba oil 3.00 3.00 3.00 3.00 3.00 3.00 12. Cyclopentasiloxane 3.00 3.00 3.00 3.00 3.00 3.00 13. Dimethicone 0.30 0.30 0.30 0.30 0.30 0.30 14. Butylated hydroxytoluene 0.02 0.02 0.02 0.02 0.02 0.02 C phase
15. Triethanolamine 0.29 0.29 0.29 0.29 0.29 0.29 16. Purified water 3.00 3.00 3.00 3.00 3.00 3.00 D phase

17. Phenoxyethanol 0.50 0.50 0.50 0.50 0.50 0.50 18. Spices a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount 19. Example 1 5 - - - - - 20. Example 2 - 5 - - - - 21. Example 3 - - 5 - - - 22. Example 4 - - - 5 - - 23. Example 5 - - - - 5 -

< Example 16-20> Mask packs Cosmetics Composition manufacturing

The mask pack cosmetic compositions of Examples 16 to 20 and the mask pack cosmetic compositions of Comparative Example 2, to which Examples 1 to 5 were respectively added, were prepared according to the following Table 3. A phase composed of raw materials 1 to 6, B phase composed of raw materials 7 to 9, and C phase composed of raw materials 10 to 13 were uniformly heated to 80 ° C., and then B phase and C phase were slowly added to phase A And emulsified at 3000 rpm for 7 minutes. The prepared emulsion was neutralized with 3200 rpm for 7 minutes by adding D phase, which is a neutralizing agent composed of 14 to 15 raw materials. After the contents were cooled to 40 ° C, the remaining ingredients were added and stirred to prepare a final mask pack cosmetic composition.

Item Raw material name Example 16 Example 17 Example 18 Example 19 Example 20 Comparative Example 1 A phase

1. Glycerin 5 5 5 5 5 5 2. IP-glucan 0.2 0.2 0.2 0.2 0.2 0.2 3. D-Panthenol 0.1 0.1 0.1 0.1 0.1 0.1 4. Methylparaben 0.15 0.15 0.15 0.15 0.15 0.15 5. Disodium di 0.02 0.02 0.02 0.02 0.02 0.02 6. Purified water Balance Balance Balance Balance Balance Balance B phase

7. Zanthan gum 0.32 0.32 0.32 0.32 0.32 0.32 8. Butch Renkliak 5.2 5.2 5.2 5.2 5.2 5.2 9. Carbomer 1% aqueous solution 0.08 0.08 0.08 0.08 0.08 0.08 C phase

10. Polysorbate 80 0.2 0.2 0.2 0.2 0.2 0.2 11. Phenoxyethanol 0.2 0.2 0.2 0.2 0.2 0.2 12. Spices a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount 13. Tocopheryl acetate 0.02 0.02 0.02 0.02 0.02 0.02 D phase 14. Triethanolamine 0.1 0.1 0.1 0.1 0.1 0.1 15. Purified water 2 2 2 2 2 2 E phase

16. Safflower extract, ethanol dissolved in ethanol 0.02 0.02 0.02 0.02 0.02 0.02 17. Hatchery extract 0.03 0.03 0.03 0.03 0.03 0.03 18. Rosemary extract 0.05 0.05 0.05 0.05 0.05 0.05 19. Example 1 5 - - - - - 20. Example 2 - 5 - - - - 21. Example 3 - - 5 - - - 22. Example 4 - - - 5 - - 23. Example 5 - - - - 5 -

< Experimental Example 1> Antioxidant activity test

For the antioxidant activity experiments, the extracts of Examples 1 to 5 were adjusted to a concentration of 10,000 mg / L using distilled water and ethanol. To increase the accuracy of the measurement results, concentrations of 10, 100, 1000 and 5000 mg / And the antioxidative capacity of each concentration was evaluated.

<1-1> DPPH Measurement of radical scavenging activity

To measure the DPPH radical scavenging activity, 0.2 mM DPPH (2,2-Diphenyl-1-picryl-hydrazyl) solution was prepared using methanol and then mixed with each sample prepared at each concentration. The mixture was incubated at 37 ° C for 30 minutes, and the absorbance at 515 nm was analyzed using a UV-Vis spectrophotometer. As a control, the DPPH solution without the sample was used in the equation for calculating the result by analyzing the absorbance under the same conditions. The equation for confirming the DPPH radical scavenging activity using the absorbance value is as follows.

DPPH radical scavenging activity (%) = [(Abs _DPPH Abs _Sample ) / Abs _DPPH ] 占 100

As a result, as shown in Table 4 and FIG. 1, DPPH radical scavenging activity was higher in the order of 60% ethanol extract, 30% ethanol extract, 80% ethanol extract, 99% ethanol extract and distilled water extract.

Concentration (mg / L) Distilled water Ethanol 30% Ethanol 60% Ethanol 80% Ethanol 99% 10000 mg / L 34.5 ± 1.8 64.5 ± 0.4 77.7 ± 4.7 62.2 ± 1.9 39.1 ± 4.2

<1-2> ABTS Measurement of radical scavenging activity

To measure the ABTS radical scavenging activity, 7 mM ABTS (2,2-Azino-bis- (3-ethylbenzothiazoline-6-sulfonic acid)) was prepared by using a third distilled water and 2.45 mM potassium persulfate ). This was stored for 12 hours in a dark room at room temperature, and the absorbance was adjusted to 1.0 at a wavelength of 734 nm using UV-Vis spectrophotometer before reaction with each sample. The prepared ABTS solution and sample were mixed and reacted at room temperature for 7 minutes. The absorbance of the mixed solution was analyzed under the above conditions and the result was calculated. The formula for calculating the radical scavenging activity of ABTS is as follows.

ABTS radical scavenging activity (%) = [(Abs _ABTS Abs _Sample ) / Abs _ABTS ] x 100

As a result, as shown in Table 5 and FIG. 2, ABTS radical scavenging activity was higher in the order of 30% ethanol extract, 60% ethanol extract, 80% ethanol extract, 99% ethanol extract and distilled water extract.

Concentration (mg / L) Distilled water Ethanol 30% Ethanol 60% Ethanol 80% Ethanol 99% 10000 mg / L 50.4 ± 1.3 57.5 ± 1.7 54.7 ± 0.8 52.2 ± 0.7 33.2 ± 0.6

< Experimental Example 2> Total polyphenol and total flavonoid content measurement

To measure the total polyphenol content, 1 ml of methanol and 5 ml of distilled water were added to 1 ml of each concentration extract and mixed. Then, 0.5 ml of 1 N-folin-ciocalteu's phenol reagent was added, and the mixture was allowed to stand for 5 minutes. 1 ml of 5% Na ₂ CO ₃ was added to a stationary sample, and the reaction was allowed to proceed for 1 hour in a dark place. The absorbance at 725 nm was analyzed using a UV-Vis spectrophotometer. A standard curve was prepared using Gallic acid in the same manner as above, and the total polyphenol content was confirmed by comparing the absorbance of each sample. The results obtained are shown as GAE (gallic acid equivalent) per gram of sample.

To determine the total flavonoid content, 0.1 ml of 10% Al (NO ₃ ) ₃ and 1 M KCH ₃ CO ₂ were added to 0.5 ml of a mixture of methanol extract and methanol at a ratio of 1: 9 . 4.3 ml of methanol was added thereto, and the mixture was reacted at room temperature for 40 minutes. The absorbance of the reacted sample was measured at a wavelength of 415 nm using a UV-Vis spectrophotometer. The total flavonoid content was verified against the standard curve plotted in the same manner using quercetin. The results obtained are shown as QE (quercetin equivalent) per gram of sample.

The accuracy of the results was improved by calculating the dilution factor for each concentration.

As a result, total polyphenol and total flavonoid contents were as high as 60% ethanol extract, 80% ethanol extract, 30% ethanol extract, 99% ethanol extract and distilled water extract as shown in Table 6 and FIGS.

Total polyphenol (mg GAE / g) Total flavonoid (mg QE / g) Distilled water 60.83 + - 0.75 40.94 + 0.25 Ethanol 30% 65.49 + - 1.06 44.32 + - 1.75 Ethanol 60% 67.35 + 1.13 46.35 ± 1.88 Ethanol 80% 62.83 + - 2.13 43.96 + 0.36 Ethanol 99% 48.61 + 1.88 33.86 ± 1.21

Claims

An anti-aging cosmetic composition comprising an extract of Citrusfortunella microcarpa as an active ingredient.

The anti-aging cosmetic composition according to claim 1, wherein the anti-aging cosmetic composition comprises 0.001 to 50% by weight of a Kalamanshi extract based on the total weight of the cosmetic composition.

The cosmetic composition for anti-aging according to claim 1, wherein the calamansi extract is extracted with at least one solvent selected from the group consisting of water or an aqueous ethanol solution.

The anti-aging cosmetic composition according to claim 3, wherein the aqueous ethanol solution is an aqueous solution of 30 to 99% ethanol.

The anti-aging cosmetic composition according to claim 1, wherein the anti-aging cosmetic composition is at least one selected from the group consisting of lotion, skin lotion, moisturizing lotion, nutritional lotion, skin toner, essence, foundation, nutritional cream, hand cream, Wherein the cosmetic composition is formulated into any one selected from the group consisting of sunflower cream, soap, body lotion, body cream, body cleanser, cleansing lotion, cleansing foam, shampoo, rinse or conditioner.