JPH05163135A - Beautifying cosmetic composition - Google Patents

Abstract

PURPOSE:To obtain a composition for beautifying cosmetic, having safety and high effects. CONSTITUTION:A beautifying cosmetic composition comprising at least one of an extract of Auricularia auricula-juade Quel. of the family Auriculariaceae, an extract of Wasabia japonica Matsum. of the family Cruciferae and an extract of Fogopyrum esculentum Moench of the family Polygonaceae as an active ingredient. The extract extremely inhibits melanism without showing cytotoxicity. Consequently, the beautifying cosmetic composition comprising at least one of these extracts and their purified substances has safety and high beautifying effects.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a whitening cosmetic composition having an excellent whitening effect, and more specifically, it is particularly effective in preventing or treating spots, freckles and the like, and has an excellent whitening effect. The present invention relates to a whitening cosmetic composition having both stability and high safety to the skin.

[0002]

2. Description of the Related Art Conventionally, hydroquinone and its glycoside, for the purpose of preventing or treating spots, freckles, etc.,
There has been proposed a whitening cosmetic composition containing substances such as kojic acid and its derivatives, ascorbic acid and its derivatives, thiol compounds, and various animal and plant extracts as a whitening component.

[0003]

However, various problems remain in these cosmetics, and there is a problem in terms of practicality. For example, hydroquinone as a whitening ingredient has a problem in safety, and hydroquinone glycosides, kojic acid and its derivatives, ascorbic acid and its derivatives, etc. have a high polarity for use as a blending ingredient of cosmetics. There is a problem that it is too much.

Further, thiol compounds such as glutathione and cysteine still have problems in stability after compounding, and other animal and plant extracts such as placenta extract,
Aloe extract and other formulations are known, but their whitening effect was not satisfactory.

[0005]

[Means for Solving the Problems] In order to find a substance having an excellent whitening effect, the present inventors have screened various food materials with a melanin production inhibition test using a B16 melanoma strain of a mouse as an index. went. As a result, Asteraceae (Auricularia a)
uricula-judae) extract, extract of Wasabia japonica,
Capsicum capsicum and Capricorn buckwheat (Fagopyrum esculentu)
It was found that the extract component of m) strongly suppresses melanin production and has extremely weak cytotoxicity, and is extremely effective as a whitening component of cosmetics.

The present invention has been completed based on the above findings, and provides a whitening cosmetic composition containing at least one of the above extracts as an active ingredient.

In the present invention, the whitening cosmetic composition means
A skin cosmetic composition effective for the prevention or treatment of spots, freckles, etc., a lotion as a quasi drug, an emulsion,
It includes external cosmetics such as creams, packs, medicated cosmetics such as soaps and lotions as pharmaceuticals, emulsions, creams, ointments and the like.

All of the food materials used in the present invention have been accepted by humanity as food materials over a long history and have no problem at least in terms of oral toxicity. For example, the mushrooms used in the present invention are
It is an edible mushroom belonging to the basidiomycete fungus family and is said to be edible in many countries around the world. In China, it is called a wood ear and is an essential ingredient for Chinese cuisine. However, it is completely unknown that this product is effective as a whitening agent.

The horseradish used in the present invention is not only used as a grated horseradish but also as a spicy sauce by grazing the rhizome.
As a local analgesic for rheumatism and neuralgia, it is also known to be used as a folk medicine in which grated horseradish is applied locally, but it is not known at all to be effective as a whitening agent.

The capsicum used in the present invention is used as a spicy substance after drying its fruit, and as peppercorn, its use as a skin stimulant and a pungency stomachic agent is described in the Japanese Pharmacopoeia, and it is also a folk medicine. It is used as a preventive and therapeutic agent for frost burning, but it is not known at all that it is effective as a whitening agent.

It is described that the buckwheat used in the present invention has its fruits edible and that rutin is purified from its dried foliage, but it is completely known that this is effective as a whitening agent. Has not been done.

The solvent used when extracting the active ingredient from these raw materials such as plants is not particularly limited as long as the active ingredient can be effectively extracted, but water or acetone, methanol, It is preferable to use an organic solvent miscible with water such as lower alcohols such as ethanol and propanol, a mixed solution thereof, ethyl acetate or the like.

These extracts can be obtained by directly extracting commercially available fungus, horseradish, capsicum, buckwheat, etc. with a solvent, but preferably, in order to enhance extraction efficiency, these raw materials are finely divided. It is desirable to cut and then extract with a solvent. Extraction is 1 g of finely chopped raw material
To 5 to 100 ml, preferably 10 to 20 ml
It is desirable to use the above solvent at room temperature for 1 to 1 month, preferably 2 to 5 days, in 2 to 4 times. Further, in order to increase the concentration of the active ingredient in the extract, the obtained extract may be further subjected to such means as concentration, liquid-liquid partitioning, adsorption chromatography, normal phase or reverse phase chromatography, if desired. It is possible.

The whitening cosmetic composition of the present invention comprises the extract obtained as described above and components generally used in cosmetics such as oily components, surfactants, ultraviolet absorbers, lower alcohols, Preservatives, bactericides, colorants, powders, fragrances, water-soluble polymers, buffers and the like are prepared according to the dosage form, and appropriately mixed within a range that does not impair the effects of the present invention.

The amount of the above-mentioned extract compounded in the whitening cosmetic composition of the present invention is preferably determined in consideration of the melanin production inhibitory action, the skin keratin permeability, etc. It is 0.01 to 50% by weight, preferably 0.1 to 20% by weight.

[0016]

It is not yet known how the above-mentioned extract, which is the active ingredient of the present invention, acts to have a whitening effect. However, in view of the fact that the above extract strongly suppresses melanin production without showing cytotoxicity in a melanin production inhibition test using a mouse B16 melanoma strain, in any step from tyrosine to melanin production,
It is expected to inhibit this reaction.

Next, the present invention will be described in more detail by way of examples, but the scope of the present invention is not limited to these.

[0018]

【Example】

Example 1 Preparation of a fungus extract: 10 g of dried black fungus (purchased from a Japanese grocery store) was cut into small pieces, and 200 ml of ethanol was added thereto at room temperature.
Extracted for 48 hours. The extract was filtered off and the residue was re-extracted in the same manner by adding 200 ml of ethanol. The extracts were collected and concentrated under reduced pressure, the solvent was distilled off, 5 ml of water and 10 ml of ethyl acetate were added, and the mixture was transferred to a separatory funnel, shaken vigorously, and left standing to separate into two layers. The ethyl acetate layer was collected. This was dried over anhydrous sodium sulfate and then concentrated under reduced pressure to obtain 260 mg of an extract.

[0019] grated in Example 2 adjusted wasabi rhizome (purchased from Seika quotient) of wood ear extract sliding unit of 20g, ethanol extract as in Example 1, under reduced pressure followed by concentration of ethyl acetate redissolution, the extract 140mg Obtained.

Example 3 Preparation of capsicum extract 20 g of red chili pepper (purchased from a grocery store) was finely chopped, extracted with ethanol in the same manner as in Example 1, transferred to ethyl acetate and concentrated under reduced pressure to obtain 170 mg of extract. It was

Example 4 Preparation of buckwheat extract 10 g of buckwheat seeds (buckwheat rice, purchased from a grocery store) from which half-boiled shells were removed were finely crushed using a coffee mill, and 100 ml of 50% ethanol was added. At room temperature, 4
Extracted for 8 hours. The extract is filtered off and the residue is 50%
100 ml of ethanol was added and re-extracted in the same manner. The extracts were collected, concentrated under reduced pressure, and the solvent was distilled off.
In the same manner as in Example 1, the extract was dissolved in ethyl acetate and then concentrated under reduced pressure to extract 310
mg was obtained.

Example 5 Melanin production inhibition test The melanin production inhibition test was carried out as follows using mouse melanoma cells. First, 1 × 10 ⁵ B16 melanoma cells were seeded in a petri dish having a diameter of 60 mm containing 5 ml of Eagle's minimal nutrient medium containing 10% (v / v) fetal bovine serum, and 5% (v / v) carbon dioxide gas was added. The cells were cultured at 37 ° C. for 24 hours in a carbon dioxide gas incubator adjusted to 1. Next, 50 μl of a sample dissolved in ethanol on this petri dish
Was added. After further culturing for 5 days under the same conditions, cells were collected by trypsin treatment, the degree of whitening was visually evaluated, and at the same time, the change in cell mass volume was visually evaluated and used as an index of cytotoxicity.

By this measuring method, inhibition of melanin production was measured at various concentrations for the fungus extract of Example 1, the horseradish extract of Example 2, the pepper extract of Example 3 and the buckwheat extract of Example 4. did. The results are shown in Table 1.
All of the extracts of the present invention showed a ++ (high degree of whitening) melanin production inhibitory effect sufficient for practical use within the range where the cell mass volume was not reduced. This indicates that the extract of the present invention is a useful whitening ingredient having both an excellent whitening effect and high safety.

[0024]

[Table 1]

Example 6 Production of Whitening Cream [Formulation] A Extract of Example 1 0.05 g Purified water 5.50 g B 3-Succinyloxyglycyrrhetinic acid dibasic sodium 0.05 g C Squalane 10.00 g Octyldodecyl myristate 8.00 g Microcrystalline wax 4.00 g Behenyl alcohol 3.00 g Lipophilic glyceryl monostearate 2.50 g Polyoxyethylene sorbitan monostearate (20 EO) 2.50 g D 1,3-butylene glycol 10.00 g Paraoxy Methyl benzoate 0.10 g Purified water 54.00 g E Fragrance 0.30 g

[Production Method] B was added to D heated to 80 to 85 ° C., and C which was heated and dissolved at 80 to 85 ° C. was added thereto with stirring with a homomixer to uniformly emulsify. It was gradually cooled to about 50 ° C. at room temperature and E and A were added. The whitening cream was manufactured by further cooling to room temperature while continuing stirring. The whitening cream thus produced had an excellent whitening effect.

Example 7 Production of Whitening Cream Instead of [Formulation] A in Example 6, Then, a whitening cream was produced in the same manner as in Example 6.

Example 8 Production of Carmine Lotion

[Production Method] About 60 g of D was added to A and uniformly dispersed with a homomixer to prepare a powder dispersion. A solution of B and C were added to this, the rest of D was added, and the mixture was uniformly dispersed with a homomixer to produce a carmine lotion. The carmine lotion produced in this manner had a hot whitening effect and an excellent whitening effect.

Example 9 Production of whitening ointment

[Manufacturing Method] Macrogol 4000 and Macrogol 400 were heated to 65 ° C. in a water bath to dissolve, and uniformly mixed to prepare a Macrogol ointment base. The solution of B was kneaded with this to produce a whitening ointment. The whitening ointment thus produced had a hot whitening effect and was excellent in whitening effect.

[0031]

EFFECTS OF THE INVENTION Extracts of the genus Asteraceae, the extract of Brassicaceae, the horseradish, the extracts of Capsicum angustifolia and the buckwheat buckwheat significantly suppress melanin production without showing cytotoxicity. Therefore, the whitening cosmetic composition of the present invention containing at least one of these extracts or purified products thereof is safe and has a high whitening effect.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁵ Identification number Reference number within the agency FI Technical indication location A61K 35/84 ADA A 7180-4C (72) Inventor Junsei Asami 1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka Prefecture 1-1-1 Suntory Ltd. Basic Research Center

Claims (1)

[Claims] 1. A whitening product comprising at least one of an extract of Chrysomelidae, an extract of Brassicaceae, a horseradish, an extract of Capsicum, and a buckwheat buckwheat as an active ingredient. Cosmetic composition.